MD10G1004000.v1.1

Protein of unknown function (DUF1336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0007793
Physical Location & Seq
Forward (+)
713509 .. 716657
3149 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1004000.v1.1.491

Sequence Viewer

Length: 948 bp
ATGGACAGCACTGGTGAAAAGAAGGAGCTTGAATGGATAGAGAGAGTGAGATCAGAAGGAGCTGTTCCACTTCTTGATCCAGACAATTGTCGAAATGGTTGGGCTTCTCCACCTGGGGACAAGTTCTTAGTTAGAGGTCCTGACTACTTGTCAACCAAAGTTAAAGTTCCTGCTGGTGAATATCTTCTAAAGCCTCTTGGTTTCGATTGGATTAGAAGTTCGACAAAGATTGGGGAGGTCTTAAAACATCCAAACAGTCGTGTCAAGAAGGCTATTGAGGAGGAGTTCCCGACTGGTGATAAGCCTTTCGTTTGGGCTTTCAATCTTCAAGTTCCGAATAAGGATAATTACAGTGCTGTGGCGTATTTTACAACCAGAGAGCCTATTCCTGAGGGGTCTTTGATGGACCAGTTCTTGAAGGGTGATGATGGGTTTAGGAATTCAAGGCTTAAATTGATTGCCAACATTGTCAAGGGCCCTTGGATCGTGAGGAAAGCAGTTGGGGAGCAGGCCATATGCATAATTGGGCGTGCCCTTTCTTGTTATTACTGTGCATCAGATAGTTTCTTAGAAGTAGACGTGGATATTGGGTCTTCCATGGTTGCAAGTGCAATAGTTCATCTGGCATTTGGTTATATCACAACTCTGACTGTTGACCTTGCATTTCTCATCGAGAGCCAAACTGAATCAGAGCTTCCAGAACAACTTTTAGGTGCTGTAAGATTCTCTGAACTAGACCCTGCGGTGGCAAGGCCAACTGAACCATATCTTGGTAGGAGTACTGGCAGTTTGCAGTCTAATCTGCCTATACGGTTATGGAAGTCAATTGGACAAGGATTTTCCCACATTCTTCATCCAGGTGACCATGAAAATGGGTCTAGCTCGGGGTCAGCACATGTTAATGGGATTGGAGATAATGAAAACAGCTTAAAAAATGGACCAGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

316

Amino Acids

34.52

Weight (kDa)

5.49

Isoelectric Point (pI)

38.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 34 - 246 1.6e-78 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 770
AccI GTMKAC 1 cut(s) 576
AciI CCGC 1 cut(s) 743
AclWI GGATC 2 cut(s) 71, 491
AcsI RAATTY 1 cut(s) 439
AfaI GTAC 1 cut(s) 781
AfiI CCNNNNNNNGG 2 cut(s) 745, 770
AflIII ACRYGT 1 cut(s) 895
AgsI TTSAA 5 cut(s) 32, 322, 329, 418, 444
AhdI GACNNNNNGTC 1 cut(s) 148
AjiI CACGTC 1 cut(s) 580
AjnI CCWGG 3 cut(s) 112, 856, 940
AjuI GAANNNNNNNTTGG 2 cut(s) 753, 785
AloI GAACNNNNNNTCC 2 cut(s) 202, 234
AluBI AGCT 5 cut(s) 28, 62, 694, 882, 927
AluI AGCT 5 cut(s) 28, 62, 694, 882, 927
AlwI GGATC 2 cut(s) 71, 491
Ama87I CYCGRG 1 cut(s) 883
AoxI GGCC 3 cut(s) 475, 510, 752
ApaI GGGCCC 1 cut(s) 479
ApoI RAATTY 1 cut(s) 439
Asp700I GAANNNNTTC 1 cut(s) 183
AspS9I GGNCC 5 cut(s) 137, 406, 475, 476, 938
AsuHPI GGTGA 5 cut(s) 26, 188, 308, 434, 872
AvaI CYCGRG 1 cut(s) 883
AvaII GGWCC 3 cut(s) 137, 406, 938
AxyI CCTNAGG 1 cut(s) 390
BaeGI GKGCMC 2 cut(s) 479, 535
BanII GRGCYC 1 cut(s) 479
BbsI GAAGAC 1 cut(s) 585
BccI CCATC 2 cut(s) 397, 422
BciT130I CCWGG 3 cut(s) 114, 858, 942
BfaI CTAG 2 cut(s) 734, 879
BmcAI AGTACT 1 cut(s) 781
Bme1390I CCNGG 3 cut(s) 114, 858, 942
Bme18I GGWCC 3 cut(s) 137, 406, 938
BmeRI GACNNNNNGTC 1 cut(s) 148
BmeT110I CYCGRG 1 cut(s) 883
BmgBI CACGTC 1 cut(s) 580
BmgT120I GGNCC 5 cut(s) 137, 406, 475, 476, 938
BmiI GGNNCC 1 cut(s) 477
BmrFI CCNGG 3 cut(s) 114, 858, 942
BmsI GCATC 1 cut(s) 563
BoxI GACNNNNGTC 1 cut(s) 87
BpiI GAAGAC 1 cut(s) 585
BsaJI CCNNGG 3 cut(s) 113, 479, 597
Bsc4I CCNNNNNNNGG 2 cut(s) 745, 770
Bse1I ACTGG 4 cut(s) 16, 298, 409, 787
Bse21I CCTNAGG 1 cut(s) 390
BseBI CCWGG 3 cut(s) 114, 858, 942
BseDI CCNNGG 3 cut(s) 113, 479, 597
BseGI GGATG 2 cut(s) 247, 853
BseLI CCNNNNNNNGG 2 cut(s) 745, 770
BseMII CTCAG 1 cut(s) 381
BseNI ACTGG 4 cut(s) 16, 298, 409, 787
BseRI GAGGAG 2 cut(s) 293, 296
BseSI GKGCMC 2 cut(s) 479, 535
BshFI GGCC 3 cut(s) 477, 512, 754
BsiHKCI CYCGRG 1 cut(s) 883
BslFI GGGAC 1 cut(s) 131
BslI CCNNNNNNNGG 2 cut(s) 745, 770
BsmFI GGGAC 1 cut(s) 131
BsnI GGCC 3 cut(s) 477, 512, 754
BsoBI CYCGRG 1 cut(s) 883
Bsp120I GGGCCC 1 cut(s) 475
Bsp1286I GDGCHC 2 cut(s) 479, 535
Bsp143I GATC 3 cut(s) 50, 76, 483
Bsp19I CCATGG 1 cut(s) 597
BspACI CCGC 1 cut(s) 743
BspANI GGCC 3 cut(s) 477, 512, 754
BspCNI CTCAG 1 cut(s) 382
BspLI GGNNCC 1 cut(s) 477
BspPI GGATC 2 cut(s) 71, 491
BsrI ACTGG 4 cut(s) 16, 298, 409, 787
BssECI CCNNGG 3 cut(s) 113, 479, 597
BssMI GATC 3 cut(s) 50, 76, 483
BssT1I CCWWGG 2 cut(s) 479, 597
Bst2UI CCWGG 3 cut(s) 114, 858, 942
Bst4CI ACNGT 5 cut(s) 257, 353, 551, 652, 813
BstC8I GCNNGC 2 cut(s) 510, 531
BstDEI CTNAG 3 cut(s) 127, 390, 568
BstDSI CCRYGG 1 cut(s) 597
BstEII GGTNACC 1 cut(s) 860
BstF5I GGATG 2 cut(s) 247, 853
BstKTI GATC 3 cut(s) 53, 79, 486
BstMBI GATC 3 cut(s) 50, 76, 483
BstNI CCWGG 3 cut(s) 114, 858, 942
BstNSI RCATGY 1 cut(s) 899
BstPAI GACNNNNGTC 1 cut(s) 87
BstPI GGTNACC 1 cut(s) 860
BstSCI CCNGG 3 cut(s) 112, 856, 940
BstSLI GKGCMC 2 cut(s) 479, 535
BstV2I GAAGAC 1 cut(s) 585
BstXI CCANNNNNNTGG 1 cut(s) 872
Bsu36I CCTNAGG 1 cut(s) 390
BsuRI GGCC 3 cut(s) 477, 512, 754
BtgI CCRYGG 1 cut(s) 597
BtrI CACGTC 1 cut(s) 580
BtsCI GGATG 2 cut(s) 247, 853
BtsIMutI CAGTG 2 cut(s) 9, 358
Cac8I GCNNGC 2 cut(s) 510, 531
Cfr13I GGNCC 5 cut(s) 137, 406, 475, 476, 938
Csp6I GTAC 1 cut(s) 780
CviAII CATG 3 cut(s) 598, 866, 896
CviQI GTAC 1 cut(s) 780
DdeI CTNAG 3 cut(s) 127, 390, 568
DpnI GATC 3 cut(s) 52, 78, 485
DpnII GATC 3 cut(s) 50, 76, 483
DriI GACNNNNNGTC 1 cut(s) 148
Eam1105I GACNNNNNGTC 1 cut(s) 148
Eco130I CCWWGG 2 cut(s) 479, 597
Eco24I GRGCYC 1 cut(s) 479
Eco47I GGWCC 3 cut(s) 137, 406, 938
Eco81I CCTNAGG 1 cut(s) 390
Eco88I CYCGRG 1 cut(s) 883
Eco91I GGTNACC 1 cut(s) 860
EcoO109I RGGNCCY 3 cut(s) 137, 475, 476
EcoO65I GGTNACC 1 cut(s) 860
EcoRI GAATTC 1 cut(s) 439
EcoRII CCWGG 3 cut(s) 112, 856, 940
EcoT14I CCWWGG 2 cut(s) 479, 597
EcoT22I ATGCAT 1 cut(s) 521
EcoT38I GRGCYC 1 cut(s) 479
ErhI CCWWGG 2 cut(s) 479, 597
FaeI CATG 3 cut(s) 601, 869, 899
FaqI GGGAC 1 cut(s) 131
FatI CATG 3 cut(s) 597, 865, 895
FauNDI CATATG 1 cut(s) 515
FblI GTMKAC 1 cut(s) 576
FokI GGATG 2 cut(s) 234, 840
FriOI GRGCYC 1 cut(s) 479
FspBI CTAG 2 cut(s) 734, 879
HaeIII GGCC 3 cut(s) 477, 512, 754
Hin1II CATG 3 cut(s) 601, 869, 899
HincII GTYRAC 2 cut(s) 153, 655
HindII GTYRAC 2 cut(s) 153, 655
HinfI GANTC 2 cut(s) 686, 723
HphI GGTGA 5 cut(s) 26, 188, 308, 434, 872
Hpy166II GTNNAC 3 cut(s) 153, 577, 655
Hpy188I TCNGA 6 cut(s) 55, 336, 559, 648, 691, 730
Hpy8I GTNNAC 3 cut(s) 153, 577, 655
HpyAV CCTTC 4 cut(s) 16, 50, 262, 412
HpyCH4III ACNGT 5 cut(s) 257, 353, 551, 652, 813
HpyCH4IV ACGT 1 cut(s) 579
HpyCH4V TGCA 6 cut(s) 519, 554, 605, 611, 662, 793
HpyF3I CTNAG 3 cut(s) 127, 390, 568
HpySE526I ACGT 1 cut(s) 579
Hsp92II CATG 3 cut(s) 601, 869, 899
Kzo9I GATC 3 cut(s) 50, 76, 483
LmnI GCTCC 3 cut(s) 25, 59, 505
LweI GCATC 1 cut(s) 563
MaeI CTAG 2 cut(s) 734, 879
MaeII ACGT 1 cut(s) 579
MaeIII GTNAC 1 cut(s) 860
MalI GATC 3 cut(s) 52, 78, 485
MboI GATC 3 cut(s) 50, 76, 483
MboII GAAGA 4 cut(s) 176, 317, 585, 842
MfeI CAATTG 2 cut(s) 85, 825
MhlI GDGCHC 2 cut(s) 479, 535
MluCI AATT 6 cut(s) 85, 346, 439, 452, 522, 825
MnlI CCTC 7 cut(s) 128, 204, 229, 271, 274, 385, 483
Mph1103I ATGCAT 1 cut(s) 521
MroXI GAANNNNTTC 1 cut(s) 183
MseI TTAA 5 cut(s) 162, 242, 450, 900, 929
MslI CAYNNNNRTG 3 cut(s) 858, 870, 900
MspR9I CCNGG 3 cut(s) 114, 858, 942
MunI CAATTG 2 cut(s) 85, 825
MvaI CCWGG 3 cut(s) 114, 858, 942
NcoI CCATGG 1 cut(s) 597
NdeI CATATG 1 cut(s) 515
NdeII GATC 3 cut(s) 50, 76, 483
NlaIII CATG 3 cut(s) 601, 869, 899
NlaIV GGNNCC 1 cut(s) 477
NmuCI GTSAC 1 cut(s) 860
NsiI ATGCAT 1 cut(s) 521
NspI RCATGY 1 cut(s) 899
PciI ACATGT 1 cut(s) 895
PdmI GAANNNNTTC 1 cut(s) 183
PfeI GAWTC 2 cut(s) 686, 723
PflMI CCANNNNNTGG 1 cut(s) 770
PpuMI RGGWCCY 1 cut(s) 137
PscI ACATGT 1 cut(s) 895
PshAI GACNNNNGTC 1 cut(s) 87
Psp5II RGGWCCY 1 cut(s) 137
Psp6I CCWGG 3 cut(s) 112, 856, 940
PspEI GGTNACC 1 cut(s) 860
PspGI CCWGG 3 cut(s) 112, 856, 940
PspN4I GGNNCC 1 cut(s) 477
PspOMI GGGCCC 1 cut(s) 475
PspPI GGNCC 5 cut(s) 137, 406, 475, 476, 938
PspPPI RGGWCCY 1 cut(s) 137
RsaI GTAC 1 cut(s) 781
RsaNI GTAC 1 cut(s) 780
RseI CAYNNNNRTG 3 cut(s) 858, 870, 900
SaqAI TTAA 5 cut(s) 162, 242, 450, 900, 929
Sau3AI GATC 3 cut(s) 50, 76, 483
Sau96I GGNCC 5 cut(s) 137, 406, 475, 476, 938
ScaI AGTACT 1 cut(s) 781
ScrFI CCNGG 3 cut(s) 114, 858, 942
SduI GDGCHC 2 cut(s) 479, 535
SfaNI GCATC 1 cut(s) 563
SinI GGWCC 3 cut(s) 137, 406, 938
SmiMI CAYNNNNRTG 3 cut(s) 858, 870, 900
Sse9I AATT 6 cut(s) 85, 346, 439, 452, 522, 825
SsiI CCGC 1 cut(s) 743
SspMI CTAG 2 cut(s) 734, 879
StyD4I CCNGG 3 cut(s) 112, 856, 940
StyI CCWWGG 2 cut(s) 479, 597
TaaI ACNGT 5 cut(s) 257, 353, 551, 652, 813
TaiI ACGT 1 cut(s) 582
TaqI TCGA 4 cut(s) 91, 204, 221, 672
TasI AATT 6 cut(s) 85, 346, 439, 452, 522, 825
TatI WGTACW 1 cut(s) 779
TfiI GAWTC 2 cut(s) 686, 723
Tru1I TTAA 5 cut(s) 162, 242, 450, 900, 929
Tru9I TTAA 5 cut(s) 162, 242, 450, 900, 929
TscAI CASTG 2 cut(s) 16, 358
TseFI GTSAC 1 cut(s) 860
Tsp45I GTSAC 1 cut(s) 860
TspDTI ATGAA 4 cut(s) 608, 842, 882, 933
TspRI CASTG 2 cut(s) 16, 358
Van91I CCANNNNNTGG 1 cut(s) 770
VpaK11BI GGWCC 3 cut(s) 137, 406, 938
XapI RAATTY 1 cut(s) 439
XceI RCATGY 1 cut(s) 899
XmiI GTMKAC 1 cut(s) 576
XmnI GAANNNNTTC 1 cut(s) 183
XspI CTAG 2 cut(s) 734, 879
ZrmI AGTACT 1 cut(s) 781
Zsp2I ATGCAT 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.