Rorug04G0071200

Protein of unknown function (DUF1336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0007793
Physical Location & Seq
Forward (+)
10931847 .. 10989393
57547 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0071200.1

Sequence Viewer

Length: 669 bp
ATGAAGATCAGCTTGAAGAATTACTGTGAGAGCTGGAGGATGAATGTAGAGCTGCACAACATTAGAGAGTTCCAAGTGGTGCCTGAAGAGTGCATAGGATACGTTGGAAAATATGTTACTTCGACTCAGTACAAGGTGGACTCAGAAAGGGCTATTGAGGAGTCCATTGTGTATCTAAGCACTAGCTGTAATTTGAAGAATGATGGTAGAGATGTATGGATTTTTGACATTGATGATACACTGCTCTCTACTGTGCCTTACTACAAGGGACATCATTTCGGGGGTGAGAAGTTGAACCTGACTGCTTTGGAGGAGTGGATGAGCCATGGCAAGGCACCTGCTTTGGATCACTCACTCAAACTCTTCAATGAGATCAAAGCCAGAGGGCTGCAGATCATTCTGGTTTCTTCAAGGAGAGAGCACCTTAGATCAGCCACAATAAACAACCTTCTCCATGTTGGCTTCTATGGATGGACAAGTCTCATTTTAAGGGATCCTGAGGATGAAGTGAAGGGGGTGCAAGATTTCAAAGCTGATGTGAGAAAACAATTGATGGGTGGTGGATACAGAGTTTGGGGCATATTGGGAGACCAATATAGCAGCATTGAGGGACTTCCAAGAGCCAAAAGGACATTCAAACTTCCCAACCCATTGTACTATGTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

222

Amino Acids

25.54

Weight (kDa)

7.05

Isoelectric Point (pI)

43.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acid_phosphat_B PF03767 5 - 221 1.2e-69 HAD superfamily, subfamily IIIB (Acid phosphatase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 346
Acc36I ACCTGC 1 cut(s) 346
AccB1I GGYRCC 2 cut(s) 79, 334
AclWI GGATC 3 cut(s) 354, 488, 501
AcuI CTGAAG 1 cut(s) 105
AfaI GTAC 2 cut(s) 131, 656
AfiI CCNNNNNNNGG 1 cut(s) 331
AgsI TTSAA 7 cut(s) 16, 196, 295, 367, 411, 529, 637
AjuI GAANNNNNNNTTGG 2 cut(s) 617, 649
AluBI AGCT 5 cut(s) 12, 33, 52, 186, 533
AluI AGCT 5 cut(s) 12, 33, 52, 186, 533
Alw21I GWGCWC 1 cut(s) 423
Alw26I GTCTC 2 cut(s) 485, 582
AlwI GGATC 3 cut(s) 354, 488, 501
ApeKI GCWGC 3 cut(s) 52, 388, 600
AsuHPI GGTGA 1 cut(s) 296
AxyI CCTNAGG 1 cut(s) 498
BamHI GGATCC 1 cut(s) 493
BanI GGYRCC 2 cut(s) 79, 334
Bbv12I GWGCWC 1 cut(s) 423
BbvI GCAGC 3 cut(s) 39, 375, 612
BccI CCATC 3 cut(s) 197, 465, 547
BciVI GTATCC 2 cut(s) 92, 557
BcoDI GTCTC 2 cut(s) 485, 582
BfaI CTAG 1 cut(s) 183
BfmI CTRYAG 1 cut(s) 389
BfuAI ACCTGC 1 cut(s) 346
BfuI GTATCC 2 cut(s) 92, 557
BisI GCNGC 3 cut(s) 53, 389, 601
BlsI GCNGC 3 cut(s) 54, 390, 602
BmiI GGNNCC 3 cut(s) 81, 336, 495
BpmI CTGGAG 1 cut(s) 55
BsaI GGTCTC 1 cut(s) 582
BsaJI CCNNGG 1 cut(s) 325
Bsc4I CCNNNNNNNGG 1 cut(s) 331
Bse21I CCTNAGG 1 cut(s) 498
BseDI CCNNGG 1 cut(s) 325
BseGI GGATG 4 cut(s) 45, 324, 476, 508
BseLI CCNNNNNNNGG 1 cut(s) 331
BseMII CTCAG 3 cut(s) 140, 156, 489
BseRI GAGGAG 2 cut(s) 173, 326
BseXI GCAGC 3 cut(s) 39, 375, 612
BsgI GTGCAG 1 cut(s) 38
BshNI GGYRCC 2 cut(s) 79, 334
BsiHKAI GWGCWC 1 cut(s) 423
BslFI GGGAC 2 cut(s) 282, 624
BslI CCNNNNNNNGG 1 cut(s) 331
BsmAI GTCTC 2 cut(s) 485, 582
BsmFI GGGAC 2 cut(s) 282, 624
Bso31I GGTCTC 1 cut(s) 582
Bsp1286I GDGCHC 1 cut(s) 423
Bsp143I GATC 6 cut(s) 6, 346, 372, 393, 428, 493
Bsp19I CCATGG 1 cut(s) 325
BspCNI CTCAG 3 cut(s) 139, 155, 490
BspLI GGNNCC 3 cut(s) 81, 336, 495
BspMAI CTGCAG 1 cut(s) 393
BspMI ACCTGC 1 cut(s) 346
BspPI GGATC 3 cut(s) 354, 488, 501
BspT107I GGYRCC 2 cut(s) 79, 334
BspTNI GGTCTC 1 cut(s) 582
BssECI CCNNGG 1 cut(s) 325
BssMI GATC 6 cut(s) 6, 346, 372, 393, 428, 493
BssT1I CCWWGG 1 cut(s) 325
Bst4CI ACNGT 2 cut(s) 26, 253
Bst6I CTCTTC 2 cut(s) 81, 368
BstDEI CTNAG 5 cut(s) 126, 142, 176, 425, 498
BstDSI CCRYGG 1 cut(s) 325
BstF5I GGATG 4 cut(s) 45, 324, 476, 508
BstKTI GATC 6 cut(s) 9, 349, 375, 396, 431, 496
BstMAI GTCTC 2 cut(s) 485, 582
BstMBI GATC 6 cut(s) 6, 346, 372, 393, 428, 493
BstSFI CTRYAG 1 cut(s) 389
BstV1I GCAGC 3 cut(s) 39, 375, 612
BstX2I RGATCY 1 cut(s) 493
BstYI RGATCY 1 cut(s) 493
Bsu36I CCTNAGG 1 cut(s) 498
BsuI GTATCC 2 cut(s) 92, 557
BtgI CCRYGG 1 cut(s) 325
BtsCI GGATG 4 cut(s) 45, 324, 476, 508
BtsI GCAGTG 1 cut(s) 239
BtsIMutI CAGTG 1 cut(s) 239
BveI ACCTGC 1 cut(s) 346
Csp6I GTAC 2 cut(s) 130, 655
CviAII CATG 2 cut(s) 326, 455
CviQI GTAC 2 cut(s) 130, 655
DdeI CTNAG 5 cut(s) 126, 142, 176, 425, 498
DpnI GATC 6 cut(s) 8, 348, 374, 395, 430, 495
DpnII GATC 6 cut(s) 6, 346, 372, 393, 428, 493
Eam1104I CTCTTC 2 cut(s) 81, 368
EarI CTCTTC 2 cut(s) 81, 368
Eco130I CCWWGG 1 cut(s) 325
Eco31I GGTCTC 1 cut(s) 582
Eco57I CTGAAG 1 cut(s) 105
Eco81I CCTNAGG 1 cut(s) 498
EcoT14I CCWWGG 1 cut(s) 325
ErhI CCWWGG 1 cut(s) 325
FaeI CATG 2 cut(s) 329, 458
FaiI YATR 9 cut(s) 95, 114, 217, 327, 456, 468, 581, 597, 660
FalI AAGNNNNNCTT 1 cut(s) 28
FaqI GGGAC 2 cut(s) 282, 624
FatI CATG 2 cut(s) 325, 454
Fnu4HI GCNGC 3 cut(s) 53, 389, 601
FokI GGATG 4 cut(s) 52, 331, 483, 515
Fsp4HI GCNGC 3 cut(s) 53, 389, 601
FspBI CTAG 1 cut(s) 183
GluI GCNGC 3 cut(s) 53, 389, 601
GsuI CTGGAG 1 cut(s) 55
Hin1II CATG 2 cut(s) 329, 458
HinfI GANTC 3 cut(s) 124, 140, 161
HphI GGTGA 1 cut(s) 296
Hpy166II GTNNAC 1 cut(s) 139
Hpy188I TCNGA 1 cut(s) 145
Hpy188III TCNNGA 2 cut(s) 497, 666
Hpy8I GTNNAC 1 cut(s) 139
HpyAV CCTTC 2 cut(s) 458, 505
HpyCH4III ACNGT 2 cut(s) 26, 253
HpyCH4IV ACGT 1 cut(s) 102
HpyCH4V TGCA 4 cut(s) 55, 93, 391, 520
HpyF3I CTNAG 5 cut(s) 126, 142, 176, 425, 498
HpySE526I ACGT 1 cut(s) 102
Hsp92II CATG 2 cut(s) 329, 458
Kzo9I GATC 6 cut(s) 6, 346, 372, 393, 428, 493
LpnPI CCDG 7 cut(s) 19, 96, 311, 351, 386, 394, 510
Lsp1109I GCAGC 3 cut(s) 39, 375, 612
MaeI CTAG 1 cut(s) 183
MaeII ACGT 1 cut(s) 102
MaeIII GTNAC 1 cut(s) 115
MalI GATC 6 cut(s) 8, 348, 374, 395, 430, 495
MboI GATC 6 cut(s) 6, 346, 372, 393, 428, 493
MboII GAAGA 6 cut(s) 16, 28, 98, 208, 355, 399
MfeI CAATTG 1 cut(s) 548
MflI RGATCY 1 cut(s) 493
MhlI GDGCHC 1 cut(s) 423
MluCI AATT 3 cut(s) 19, 190, 548
MlyI GAGTC 3 cut(s) 118, 134, 170
MmeI TCCRAC 1 cut(s) 85
MnlI CCTC 6 cut(s) 30, 151, 304, 377, 493, 601
MseI TTAA 1 cut(s) 488
MunI CAATTG 1 cut(s) 548
NcoI CCATGG 1 cut(s) 325
NdeII GATC 6 cut(s) 6, 346, 372, 393, 428, 493
NlaIII CATG 2 cut(s) 329, 458
NlaIV GGNNCC 3 cut(s) 81, 336, 495
PaqCI CACCTGC 1 cut(s) 346
PkrI GCNGC 3 cut(s) 54, 390, 602
PleI GAGTC 3 cut(s) 118, 134, 169
PpsI GAGTC 3 cut(s) 118, 134, 169
PspN4I GGNNCC 3 cut(s) 81, 336, 495
PstI CTGCAG 1 cut(s) 393
PsuI RGATCY 1 cut(s) 493
RsaI GTAC 2 cut(s) 131, 656
RsaNI GTAC 2 cut(s) 130, 655
SaqAI TTAA 1 cut(s) 488
SatI GCNGC 3 cut(s) 53, 389, 601
Sau3AI GATC 6 cut(s) 6, 346, 372, 393, 428, 493
SchI GAGTC 3 cut(s) 118, 134, 170
SduI GDGCHC 1 cut(s) 423
SfcI CTRYAG 1 cut(s) 389
Sse9I AATT 3 cut(s) 19, 190, 548
SspMI CTAG 1 cut(s) 183
StyI CCWWGG 1 cut(s) 325
TaaI ACNGT 2 cut(s) 26, 253
TaiI ACGT 1 cut(s) 105
TaqI TCGA 1 cut(s) 122
TasI AATT 3 cut(s) 19, 190, 548
TatI WGTACW 2 cut(s) 129, 654
Tru1I TTAA 1 cut(s) 488
Tru9I TTAA 1 cut(s) 488
TscAI CASTG 1 cut(s) 246
TseI GCWGC 3 cut(s) 52, 388, 600
TspDTI ATGAA 3 cut(s) 17, 56, 519
TspRI CASTG 1 cut(s) 246
XspI CTAG 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.