Rorug04G0071100

Protein of unknown function (DUF1336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0007793
Physical Location & Seq
Forward (+)
10930302 .. 10930996
695 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0071100.1

Sequence Viewer

Length: 246 bp
ATGGTCATAGGCACTGTCAAGAAGGGTAAGCCTGATCTGATGAAGAAGGTCCTGCCAGCTGTCATTTGGAGGCAGCGCAAGCCATGGCACCAAAAGGATGGTGTCTTCATGTACTTCGAAGATAATGCTGGTGTTATTGTTAATCCTAAGGGAGAAATGAAAGGATCTGCAATTACTGGTCTTATTGGGAAGGAGTGTGCTGATCTGTGGCCTAGGATTGCCAGTGCTGCAAATGCTATCGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

81

Amino Acids

8.92

Weight (kDa)

9.78

Isoelectric Point (pI)

16.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L14 PF00238 1 - 81 5.2e-23 Ribosomal protein L14p/L23e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 87
AclWI GGATC 1 cut(s) 172
AfaI GTAC 1 cut(s) 113
AluBI AGCT 1 cut(s) 59
AluI AGCT 1 cut(s) 59
AlwI GGATC 1 cut(s) 172
AoxI GGCC 1 cut(s) 209
ApeKI GCWGC 2 cut(s) 73, 227
AspA2I CCTAGG 1 cut(s) 212
AspLEI GCGC 1 cut(s) 78
AspS9I GGNCC 1 cut(s) 49
AsuII TTCGAA 1 cut(s) 117
AvaII GGWCC 1 cut(s) 49
AvrII CCTAGG 1 cut(s) 212
AxyI CCTNAGG 1 cut(s) 147
BanI GGYRCC 1 cut(s) 87
BbsI GAAGAC 1 cut(s) 97
BbvI GCAGC 2 cut(s) 85, 214
BccI CCATC 1 cut(s) 92
BcgI CGANNNNNNTGC 2 cut(s) 107, 141
BfaI CTAG 1 cut(s) 213
BisI GCNGC 2 cut(s) 74, 228
BlnI CCTAGG 1 cut(s) 212
BlsI GCNGC 2 cut(s) 75, 229
Bme18I GGWCC 1 cut(s) 49
BmgT120I GGNCC 1 cut(s) 49
BmiI GGNNCC 1 cut(s) 89
BpiI GAAGAC 1 cut(s) 97
Bpu14I TTCGAA 1 cut(s) 117
BsaJI CCNNGG 2 cut(s) 83, 212
Bse1I ACTGG 2 cut(s) 181, 222
Bse21I CCTNAGG 1 cut(s) 147
BseDI CCNNGG 2 cut(s) 83, 212
BseGI GGATG 1 cut(s) 103
BseNI ACTGG 2 cut(s) 181, 222
BseXI GCAGC 2 cut(s) 85, 214
BshFI GGCC 1 cut(s) 211
BshNI GGYRCC 1 cut(s) 87
BsnI GGCC 1 cut(s) 211
Bsp119I TTCGAA 1 cut(s) 117
Bsp143I GATC 3 cut(s) 34, 164, 202
Bsp19I CCATGG 1 cut(s) 83
BspANI GGCC 1 cut(s) 211
BspLI GGNNCC 1 cut(s) 89
BspPI GGATC 1 cut(s) 172
BspT104I TTCGAA 1 cut(s) 117
BspT107I GGYRCC 1 cut(s) 87
BsrI ACTGG 2 cut(s) 181, 222
BssECI CCNNGG 2 cut(s) 83, 212
BssMI GATC 3 cut(s) 34, 164, 202
BssT1I CCWWGG 2 cut(s) 83, 212
Bst4CI ACNGT 1 cut(s) 16
BstBI TTCGAA 1 cut(s) 117
BstC8I GCNNGC 2 cut(s) 57, 80
BstDEI CTNAG 1 cut(s) 147
BstDSI CCRYGG 1 cut(s) 83
BstF5I GGATG 1 cut(s) 103
BstHHI GCGC 1 cut(s) 78
BstKTI GATC 3 cut(s) 37, 167, 205
BstMBI GATC 3 cut(s) 34, 164, 202
BstMWI GCNNNNNNNGC 3 cut(s) 79, 227, 233
BstV1I GCAGC 2 cut(s) 85, 214
BstV2I GAAGAC 1 cut(s) 97
BstX2I RGATCY 1 cut(s) 164
BstXI CCANNNNNNTGG 1 cut(s) 98
BstYI RGATCY 1 cut(s) 164
Bsu36I CCTNAGG 1 cut(s) 147
BsuRI GGCC 1 cut(s) 211
BtgI CCRYGG 1 cut(s) 83
BtsCI GGATG 1 cut(s) 103
BtsIMutI CAGTG 2 cut(s) 12, 229
Cac8I GCNNGC 2 cut(s) 57, 80
CfoI GCGC 1 cut(s) 78
Cfr13I GGNCC 1 cut(s) 49
Csp6I GTAC 1 cut(s) 112
CviAII CATG 2 cut(s) 84, 109
CviJI RGCY 4 cut(s) 31, 59, 82, 211
CviKI_1 RGCY 4 cut(s) 31, 59, 82, 211
CviQI GTAC 1 cut(s) 112
DdeI CTNAG 1 cut(s) 147
DpnI GATC 3 cut(s) 36, 166, 204
DpnII GATC 3 cut(s) 34, 164, 202
Eco130I CCWWGG 2 cut(s) 83, 212
Eco47I GGWCC 1 cut(s) 49
Eco81I CCTNAGG 1 cut(s) 147
EcoO109I RGGNCCY 1 cut(s) 49
EcoT14I CCWWGG 2 cut(s) 83, 212
ErhI CCWWGG 2 cut(s) 83, 212
FaeI CATG 2 cut(s) 87, 112
FaiI YATR 3 cut(s) 8, 85, 110
FatI CATG 2 cut(s) 83, 108
Fnu4HI GCNGC 2 cut(s) 74, 228
FokI GGATG 1 cut(s) 110
Fsp4HI GCNGC 2 cut(s) 74, 228
FspBI CTAG 1 cut(s) 213
GlaI GCGC 1 cut(s) 77
GluI GCNGC 2 cut(s) 74, 228
HaeIII GGCC 1 cut(s) 211
HhaI GCGC 1 cut(s) 78
Hin1II CATG 2 cut(s) 87, 112
Hin6I GCGC 1 cut(s) 76
HinP1I GCGC 1 cut(s) 76
Hpy188I TCNGA 1 cut(s) 39
Hpy188III TCNNGA 1 cut(s) 19
HpyAV CCTTC 3 cut(s) 16, 40, 184
HpyCH4III ACNGT 1 cut(s) 16
HpyCH4V TGCA 2 cut(s) 170, 230
HpyF10VI GCNNNNNNNGC 3 cut(s) 79, 227, 233
HpyF3I CTNAG 1 cut(s) 147
Hsp92II CATG 2 cut(s) 87, 112
HspAI GCGC 1 cut(s) 76
Kzo9I GATC 3 cut(s) 34, 164, 202
LpnPI CCDG 6 cut(s) 45, 65, 69, 114, 162, 235
Lsp1109I GCAGC 2 cut(s) 85, 214
MaeI CTAG 1 cut(s) 213
MalI GATC 3 cut(s) 36, 166, 204
MboI GATC 3 cut(s) 34, 164, 202
MboII GAAGA 3 cut(s) 55, 97, 131
MflI RGATCY 1 cut(s) 164
MluCI AATT 1 cut(s) 171
MnlI CCTC 1 cut(s) 63
MseI TTAA 2 cut(s) 141, 244
MspA1I CMGCKG 1 cut(s) 59
MwoI GCNNNNNNNGC 3 cut(s) 79, 227, 233
NcoI CCATGG 1 cut(s) 83
NdeII GATC 3 cut(s) 34, 164, 202
NlaIII CATG 2 cut(s) 87, 112
NlaIV GGNNCC 1 cut(s) 89
NspV TTCGAA 1 cut(s) 117
PkrI GCNGC 2 cut(s) 75, 229
PpuMI RGGWCCY 1 cut(s) 49
Psp5II RGGWCCY 1 cut(s) 49
PspN4I GGNNCC 1 cut(s) 89
PspPI GGNCC 1 cut(s) 49
PspPPI RGGWCCY 1 cut(s) 49
PsuI RGATCY 1 cut(s) 164
PvuII CAGCTG 1 cut(s) 59
RsaI GTAC 1 cut(s) 113
RsaNI GTAC 1 cut(s) 112
SaqAI TTAA 2 cut(s) 141, 244
SatI GCNGC 2 cut(s) 74, 228
Sau3AI GATC 3 cut(s) 34, 164, 202
Sau96I GGNCC 1 cut(s) 49
SetI ASST 2 cut(s) 51, 61
SfuI TTCGAA 1 cut(s) 117
SinI GGWCC 1 cut(s) 49
Sse9I AATT 1 cut(s) 171
SspMI CTAG 1 cut(s) 213
StyI CCWWGG 2 cut(s) 83, 212
TaaI ACNGT 1 cut(s) 16
TaqI TCGA 1 cut(s) 117
TasI AATT 1 cut(s) 171
TatI WGTACW 1 cut(s) 111
Tru1I TTAA 2 cut(s) 141, 244
Tru9I TTAA 2 cut(s) 141, 244
TscAI CASTG 2 cut(s) 19, 229
TseI GCWGC 2 cut(s) 73, 227
TspDTI ATGAA 3 cut(s) 56, 97, 173
TspRI CASTG 2 cut(s) 19, 229
VpaK11BI GGWCC 1 cut(s) 49
XcmI CCANNNNNNNNNTGG 1 cut(s) 63
XmaJI CCTAGG 1 cut(s) 212
XspI CTAG 1 cut(s) 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.