Rw4G011270

Protein of unknown function (DUF1336)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0007793
Physical Location & Seq
Forward (+)
26765894 .. 26769113
3220 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G011270.1

Sequence Viewer

Length: 954 bp
ATGGCCAACACCGGAGCCGAAAGGGAACCTGAATGGATACAAAGAGTGAGATCAGAGGGAGCTGTTCCGCTTGTTGATGCAGACACAGGTCAAAATGGCTGGGCTTCTCCACCTGGACACAAGTTCATGGTCAGAGGCCCGGATTACTTGTCGACCAAGGTTAAAATTCCTGCTGGTGCTTGTCTTCTAAAGCCTCTTGGTTTCGATTGGATTAAAAGCTCTACAAAGATTACAGAGATCTTAAAACATCCTAACAGTCGTGTCAGGAAGGCTATTGAGGATGAGTTTCGGTCAGGTAATAAGCCTTTTGTTTGGGCTTTCAATCTTCAAGTTCCTAGCAAGGATAACTATAGTGCGGTGGCATATTTTACAACCACAGAGCCTATCCCTGAGGGGTCTTTGATGGACCAGTTTCTGAAAGGTGATGATGGCTTTAGGAATTCGAGGCTTAAGTTGATTGCCAACATTGTCAAGGGCCCTTGGATTGTAAGAAAAGCAGTTGGGGAGCAGGCCATATGCATAATTGGTCGTGCCCTTTCTTGTAGCTATAGTGTATCAGACACTTTCCTAGAAGTAGATGTAGATATTGGATCTTCCATGGTTGCAAGTGCAATAGTTCATCTTGCATTTGGTTATATCACAACATTGACTGTAGACCTTGCCTTTCTGATTGAGGCTCAAACTGAGTCAGAGCTTCCAGAACAGCTTTTAGGTGCTGTAAGATTCTCTGACCTAGACCCTGCTGTGGCTCGGCCTGCTGAGCCATCATCTGATAGGAGTCTTGGCACTTTGCAGTCTTCTCTGCCTACAAGACTATGGAAATCACTTGGGCAGGGTTTCTCCCATATTCTTCATCCAGGTCCCCAGGAAAATGGCTCTAGCTCAGGCTCACCACATGCTAATGGAACTGTAGATAATAAAGAAGGCAGTTTTGAAGAGATTAACAAAGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1336

Protein Analysis

317

Amino Acids

34.43

Weight (kDa)

5.75

Isoelectric Point (pI)

44.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR2_C PF07059 34 - 246 2.9e-79 Protein ENHANCED DISEASE RESISTANCE 2, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 152, 654
AciI CCGC 2 cut(s) 68, 356
AclWI GGATC 1 cut(s) 598
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 165, 439
AfiI CCNNNNNNNGG 1 cut(s) 745
AflII CTTAAG 1 cut(s) 449
AgsI TTSAA 3 cut(s) 322, 329, 935
AjnI CCWGG 3 cut(s) 112, 856, 864
AluBI AGCT 6 cut(s) 62, 219, 546, 694, 706, 882
AluI AGCT 6 cut(s) 62, 219, 546, 694, 706, 882
AlwI GGATC 1 cut(s) 598
AlwNI CAGNNNCTG 1 cut(s) 415
AoxI GGCC 5 cut(s) 3, 136, 475, 510, 752
ApaI GGGCCC 1 cut(s) 479
ApoI RAATTY 2 cut(s) 165, 439
AspS9I GGNCC 5 cut(s) 137, 406, 475, 476, 860
AsuC2I CCSGG 1 cut(s) 140
AsuHPI GGTGA 2 cut(s) 434, 882
AvaII GGWCC 2 cut(s) 406, 860
AxyI CCTNAGG 1 cut(s) 390
BaeGI GKGCMC 2 cut(s) 479, 535
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 479
BbsI GAAGAC 2 cut(s) 176, 789
BccI CCATC 3 cut(s) 397, 422, 772
BciT130I CCWGG 3 cut(s) 114, 858, 866
BciVI GTATCC 1 cut(s) 30
BcnI CCSGG 1 cut(s) 140
BfaI CTAG 4 cut(s) 336, 569, 734, 879
BfmI CTRYAG 4 cut(s) 349, 547, 651, 909
BfrI CTTAAG 1 cut(s) 449
BfuI GTATCC 1 cut(s) 30
BglII AGATCT 1 cut(s) 237
BlpI GCTNAGC 1 cut(s) 759
Bme1390I CCNGG 4 cut(s) 114, 140, 858, 866
Bme18I GGWCC 2 cut(s) 406, 860
BmgT120I GGNCC 5 cut(s) 137, 406, 475, 476, 860
BmiI GGNNCC 4 cut(s) 16, 27, 477, 862
BmrFI CCNGG 4 cut(s) 114, 140, 858, 866
BmsI GCATC 1 cut(s) 67
BoxI GACNNNNGTC 1 cut(s) 87
BpiI GAAGAC 2 cut(s) 176, 789
Bpu10I CCTNAGC 1 cut(s) 883
Bpu1102I GCTNAGC 1 cut(s) 759
BpuMI CCSGG 1 cut(s) 140
BsaJI CCNNGG 4 cut(s) 156, 479, 597, 864
BsaWI WCCGGW 1 cut(s) 11
Bsc4I CCNNNNNNNGG 1 cut(s) 745
Bse1I ACTGG 1 cut(s) 409
Bse21I CCTNAGG 1 cut(s) 390
BseBI CCWGG 3 cut(s) 114, 858, 866
BseDI CCNNGG 4 cut(s) 156, 479, 597, 864
BseGI GGATG 3 cut(s) 247, 286, 853
BseLI CCNNNNNNNGG 1 cut(s) 745
BseMII CTCAG 4 cut(s) 381, 675, 750, 897
BseNI ACTGG 1 cut(s) 409
BseSI GKGCMC 2 cut(s) 479, 535
BseYI CCCAGC 1 cut(s) 99
BshFI GGCC 5 cut(s) 5, 138, 477, 512, 754
BsiSI CCGG 2 cut(s) 12, 140
BslFI GGGAC 1 cut(s) 846
BslI CCNNNNNNNGG 1 cut(s) 745
BsmFI GGGAC 1 cut(s) 846
BsnI GGCC 5 cut(s) 5, 138, 477, 512, 754
Bsp120I GGGCCC 1 cut(s) 475
Bsp1286I GDGCHC 2 cut(s) 479, 535
Bsp143I GATC 3 cut(s) 50, 237, 590
Bsp1720I GCTNAGC 1 cut(s) 759
Bsp19I CCATGG 1 cut(s) 597
BspACI CCGC 2 cut(s) 68, 356
BspANI GGCC 5 cut(s) 5, 138, 477, 512, 754
BspCNI CTCAG 4 cut(s) 382, 676, 751, 896
BspLI GGNNCC 4 cut(s) 16, 27, 477, 862
BspPI GGATC 1 cut(s) 598
BspTI CTTAAG 1 cut(s) 449
BsrI ACTGG 1 cut(s) 409
BssECI CCNNGG 4 cut(s) 156, 479, 597, 864
BssMI GATC 3 cut(s) 50, 237, 590
BssT1I CCWWGG 3 cut(s) 156, 479, 597
Bst2UI CCWGG 3 cut(s) 114, 858, 866
Bst4CI ACNGT 3 cut(s) 257, 652, 910
Bst6I CTCTTC 1 cut(s) 930
BstAFI CTTAAG 1 cut(s) 449
BstC8I GCNNGC 2 cut(s) 510, 756
BstDEI CTNAG 4 cut(s) 390, 684, 759, 883
BstDSI CCRYGG 1 cut(s) 597
BstF5I GGATG 3 cut(s) 247, 286, 853
BstKTI GATC 3 cut(s) 53, 240, 593
BstMBI GATC 3 cut(s) 50, 237, 590
BstMWI GCNNNNNNNGC 2 cut(s) 755, 760
BstNI CCWGG 3 cut(s) 114, 858, 866
BstNSI RCATGY 1 cut(s) 899
BstPAI GACNNNNGTC 1 cut(s) 87
BstSCI CCNGG 4 cut(s) 112, 138, 856, 864
BstSFI CTRYAG 4 cut(s) 349, 547, 651, 909
BstSLI GKGCMC 2 cut(s) 479, 535
BstV2I GAAGAC 2 cut(s) 176, 789
BstX2I RGATCY 2 cut(s) 237, 590
BstXI CCANNNNNNTGG 1 cut(s) 872
BstYI RGATCY 2 cut(s) 237, 590
Bsu36I CCTNAGG 1 cut(s) 390
BsuI GTATCC 1 cut(s) 30
BsuRI GGCC 5 cut(s) 5, 138, 477, 512, 754
BtgI CCRYGG 1 cut(s) 597
BtsCI GGATG 3 cut(s) 247, 286, 853
Cac8I GCNNGC 2 cut(s) 510, 756
CaiI CAGNNNCTG 1 cut(s) 415
Cfr13I GGNCC 5 cut(s) 137, 406, 475, 476, 860
CviAII CATG 3 cut(s) 127, 598, 896
DdeI CTNAG 4 cut(s) 390, 684, 759, 883
DpnI GATC 3 cut(s) 52, 239, 592
DpnII GATC 3 cut(s) 50, 237, 590
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 930
EarI CTCTTC 1 cut(s) 930
Eco130I CCWWGG 3 cut(s) 156, 479, 597
Eco24I GRGCYC 1 cut(s) 479
Eco47I GGWCC 2 cut(s) 406, 860
Eco81I CCTNAGG 1 cut(s) 390
EcoO109I RGGNCCY 3 cut(s) 475, 476, 860
EcoRI GAATTC 1 cut(s) 439
EcoRII CCWGG 3 cut(s) 112, 856, 864
EcoT14I CCWWGG 3 cut(s) 156, 479, 597
EcoT22I ATGCAT 1 cut(s) 521
EcoT38I GRGCYC 1 cut(s) 479
ErhI CCWWGG 3 cut(s) 156, 479, 597
FaeI CATG 3 cut(s) 130, 601, 899
FaqI GGGAC 1 cut(s) 846
FatI CATG 3 cut(s) 126, 597, 895
FauNDI CATATG 1 cut(s) 515
FblI GTMKAC 2 cut(s) 152, 654
FokI GGATG 3 cut(s) 234, 293, 840
FriOI GRGCYC 1 cut(s) 479
FspBI CTAG 4 cut(s) 336, 569, 734, 879
GsaI CCCAGC 1 cut(s) 103
HaeIII GGCC 5 cut(s) 5, 138, 477, 512, 754
HapII CCGG 2 cut(s) 12, 140
Hin1II CATG 3 cut(s) 130, 601, 899
HincII GTYRAC 1 cut(s) 153
HindII GTYRAC 1 cut(s) 153
HinfI GANTC 3 cut(s) 686, 723, 778
HpaII CCGG 2 cut(s) 12, 140
HphI GGTGA 2 cut(s) 434, 882
Hpy166II GTNNAC 2 cut(s) 153, 655
Hpy188I TCNGA 8 cut(s) 55, 134, 417, 559, 669, 691, 730, 772
Hpy188III TCNNGA 2 cut(s) 265, 698
Hpy8I GTNNAC 2 cut(s) 153, 655
HpyAV CCTTC 2 cut(s) 262, 917
HpyCH4III ACNGT 3 cut(s) 257, 652, 910
HpyCH4V TGCA 6 cut(s) 80, 519, 605, 611, 626, 793
HpyF10VI GCNNNNNNNGC 2 cut(s) 755, 760
HpyF3I CTNAG 4 cut(s) 390, 684, 759, 883
Hsp92II CATG 3 cut(s) 130, 601, 899
Kzo9I GATC 3 cut(s) 50, 237, 590
LmnI GCTCC 3 cut(s) 14, 59, 505
LweI GCATC 1 cut(s) 67
MaeI CTAG 4 cut(s) 336, 569, 734, 879
MalI GATC 3 cut(s) 52, 239, 592
MboI GATC 3 cut(s) 50, 237, 590
MboII GAAGA 6 cut(s) 176, 317, 585, 789, 842, 947
MflI RGATCY 2 cut(s) 237, 590
MhlI GDGCHC 2 cut(s) 479, 535
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 165, 439, 522
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 695, 787
MnlI CCTC 7 cut(s) 49, 128, 204, 271, 385, 438, 667
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 521
MscI TGGCCA 1 cut(s) 5
MseI TTAA 5 cut(s) 162, 213, 242, 450, 942
MslI CAYNNNNRTG 1 cut(s) 900
Msp20I TGGCCA 1 cut(s) 5
MspCI CTTAAG 1 cut(s) 449
MspI CCGG 2 cut(s) 12, 140
MspR9I CCNGG 4 cut(s) 114, 140, 858, 866
MvaI CCWGG 3 cut(s) 114, 858, 866
MwoI GCNNNNNNNGC 2 cut(s) 755, 760
NciI CCSGG 1 cut(s) 140
NcoI CCATGG 1 cut(s) 597
NdeI CATATG 1 cut(s) 515
NdeII GATC 3 cut(s) 50, 237, 590
NlaIII CATG 3 cut(s) 130, 601, 899
NlaIV GGNNCC 4 cut(s) 16, 27, 477, 862
NmeAIII GCCGAG 1 cut(s) 730
NsiI ATGCAT 1 cut(s) 521
NspI RCATGY 1 cut(s) 899
PfeI GAWTC 1 cut(s) 723
PleI GAGTC 2 cut(s) 694, 786
PpsI GAGTC 2 cut(s) 694, 786
PpuMI RGGWCCY 1 cut(s) 860
PshAI GACNNNNGTC 1 cut(s) 87
Psp5II RGGWCCY 1 cut(s) 860
Psp6I CCWGG 3 cut(s) 112, 856, 864
PspFI CCCAGC 1 cut(s) 99
PspGI CCWGG 3 cut(s) 112, 856, 864
PspN4I GGNNCC 4 cut(s) 16, 27, 477, 862
PspOMI GGGCCC 1 cut(s) 475
PspPI GGNCC 5 cut(s) 137, 406, 475, 476, 860
PspPPI RGGWCCY 1 cut(s) 860
PstNI CAGNNNCTG 1 cut(s) 415
PsuI RGATCY 2 cut(s) 237, 590
RseI CAYNNNNRTG 1 cut(s) 900
SalI GTCGAC 1 cut(s) 151
SaqAI TTAA 5 cut(s) 162, 213, 242, 450, 942
Sau3AI GATC 3 cut(s) 50, 237, 590
Sau96I GGNCC 5 cut(s) 137, 406, 475, 476, 860
SchI GAGTC 2 cut(s) 695, 787
ScrFI CCNGG 4 cut(s) 114, 140, 858, 866
SduI GDGCHC 2 cut(s) 479, 535
SfaNI GCATC 1 cut(s) 67
SfcI CTRYAG 4 cut(s) 349, 547, 651, 909
SinI GGWCC 2 cut(s) 406, 860
SmiMI CAYNNNNRTG 1 cut(s) 900
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
Sse9I AATT 3 cut(s) 165, 439, 522
SsiI CCGC 2 cut(s) 68, 356
SspMI CTAG 4 cut(s) 336, 569, 734, 879
StyD4I CCNGG 4 cut(s) 112, 138, 856, 864
StyI CCWWGG 3 cut(s) 156, 479, 597
TaaI ACNGT 3 cut(s) 257, 652, 910
TaqI TCGA 3 cut(s) 152, 204, 443
TaqII GACCGA 1 cut(s) 279
TasI AATT 3 cut(s) 165, 439, 522
TfiI GAWTC 1 cut(s) 723
Tru1I TTAA 5 cut(s) 162, 213, 242, 450, 942
Tru9I TTAA 5 cut(s) 162, 213, 242, 450, 942
TspDTI ATGAA 3 cut(s) 115, 608, 842
Vha464I CTTAAG 1 cut(s) 449
VpaK11BI GGWCC 2 cut(s) 406, 860
XapI RAATTY 2 cut(s) 165, 439
XceI RCATGY 1 cut(s) 899
XmiI GTMKAC 2 cut(s) 152, 654
XspI CTAG 4 cut(s) 336, 569, 734, 879
Zsp2I ATGCAT 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.