MD10G1195500.v1.1

Glycosyl transferases group 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008696
Physical Location & Seq
Reverse (-)
29396250 .. 29400464
4215 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1195500.v1.1.491

Sequence Viewer

Length: 1362 bp
ATGCAGTATCATGCTCTTTCGCTTGCCCGGCAGGCGTCTCTGGAGGTAGATATTGTTGCATATGGAGGTTCCGAACCCCATTCTGCTGTGCTGGAGCATCAGTCCATTCACATACACGAAATGCAGCAGTGGCCGACATTTTCGCGTGGCCTTCCCAGTATACTTAAACCCTTCGTGCTATTGATCAAGGCAGTGACTCAATTTTTAATGCTCCTGTGGTTTCTTTGCGTTAAGATACCCGCTCCCGATGTATTTCTGGTTCAGAATCCACCTTCTGTTCCGACCTTAGTGGCTGTAAAATGGGCAAGCTGGTTTAGGAAATCTGCCTTTATTGTTGACTGGCATAATTTTGGATATACTTTACTGGCATTGTCTCTTGGAAGAAGTAGTCAATTTGTGGCAATATACCGTTGGTTTGAGAGGCATTTTGGGAAGATGGCAGATGGTTCCCTATGTGTAACGAGGGCAATGCAACATGAATTGGCTCAAAATTGGGGGATTAAAGCCACAGTTCTATATGATCAGCCTCCTGAGTTTTTCCAACCAGCTTCACTCGAAGAAAAACATAAGTTGTTCTGCAGATTGGATAAAATTCTAAGGCAGCCTCTTGGTGTTCAAGATTGCACCAGCACTGGGGAAGAGAATACAGACATGAATGAGACTTTGTTTACCACTTTCGTTGGTACTGACGTTACTTCAAAGCCAAACAGGCCGGCACTCATAGTTAGCAGTACAAGCTGGACTCCTGATGAAGATTTTGAAATTCTCTTGGAAGCAGCTGTCATGTATGATAGGCGTGTTGCTGCAATTTTAAATGAAGATGATTCTACTAAAGAAGAGGTTATTTGGAAGGAAATCCATAGTGGGAAGCAATACTTGTACCCTAGGCTGTTATTTGTCATTACTGGTAAAGGGCCTGAAAAACAAAAATATGAAGACAAAATTAGCCGATTACACCTCAAACGCGTGGCATTTCGTACTATGTGGTTGTCTGCTGAGGACTATCCATTGCTTCTTGGATCAGCAGATCTTGGTGTATGCTTGCACACGTCCTCATCAGGGTTGGATCTTCCCATGAAGGTCGTGGACATGTTTGGTTGTGGACTGCCAGTTTGTGCTGTTTCCTACTCATGCATCAAGGAACTAGTGCAAGTTGAGAAAAATGGCCTTCTCTTTTCATCGTCTTCAGAGCTAGCTGATGAACTTTTGATGCTGTTCAAGGGTTTTCCAGATGCATGTGATTCGTTGCAGGTTCTCAGGAATGGTGCATTGGAAATGGCTTCATCTGGGAGGTGGGCTACCGAGTGGGAAGAGCATGCCAAAGCATTAATACTAGAGGTTATCTCTAAAAACTCAGATTGA

Protein Analysis

454

Amino Acids

51.13

Weight (kDa)

5.67

Isoelectric Point (pI)

40.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_trans_4_4 PF13579 11 - 169 2.9e-09 Glycosyl transferase 4-like domain
Glyco_transf_4 PF13439 11 - 169 1e-07 Glycosyltransferase Family 4
Glycos_transf_1 PF00534 293 - 401 3.3e-09 Glycosyl transferases group 1
Glyco_trans_1_4 PF13692 297 - 406 6e-10 Glycosyl transferases group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1240
AccBSI CCGCTC 1 cut(s) 242
AccI GTMKAC 1 cut(s) 160
AccII CGCG 2 cut(s) 145, 966
AciI CCGC 1 cut(s) 240
AclWI GGATC 2 cut(s) 1027, 1074
AcoI YGGCCR 1 cut(s) 131
AcsI RAATTY 2 cut(s) 591, 762
AcuI CTGAAG 1 cut(s) 1170
AcyI GRCGYC 1 cut(s) 35
AfaI GTAC 4 cut(s) 685, 733, 881, 979
AfiI CCNNNNNNNGG 2 cut(s) 633, 1059
AflIII ACRYGT 3 cut(s) 964, 1047, 1089
AgsI TTSAA 4 cut(s) 617, 699, 761, 1219
AhlI ACTAGT 1 cut(s) 1144
AjiI CACGTC 1 cut(s) 1050
AjuI GAANNNNNNNTTGG 2 cut(s) 1253, 1285
AluBI AGCT 6 cut(s) 309, 548, 738, 779, 1192, 1196
AluI AGCT 6 cut(s) 309, 548, 738, 779, 1192, 1196
Alw26I GTCTC 3 cut(s) 42, 378, 653
AlwI GGATC 2 cut(s) 1027, 1074
AoxI GGCC 5 cut(s) 131, 148, 710, 914, 1165
ApeKI GCWGC 4 cut(s) 124, 601, 776, 803
ApoI RAATTY 2 cut(s) 591, 762
ArsI GACNNNNNNTTYG 2 cut(s) 1095, 1127
AseI ATTAAT 1 cut(s) 1328
AspA2I CCTAGG 1 cut(s) 884
AspS9I GGNCC 1 cut(s) 914
AsuC2I CCSGG 1 cut(s) 28
AsuNHI GCTAGC 1 cut(s) 1192
AvrII CCTAGG 1 cut(s) 884
BaeI ACNNNNGTAYC 2 cut(s) 675, 708
BbsI GAAGAC 2 cut(s) 942, 1176
BbvCI CCTCAGC 1 cut(s) 996
BbvI GCAGC 4 cut(s) 136, 613, 788, 790
BccI CCATC 2 cut(s) 430, 437
BcgI CGANNNNNNTGC 4 cut(s) 451, 485, 1224, 1258
BclI TGATCA 2 cut(s) 183, 520
BcnI CCSGG 1 cut(s) 28
BcoDI GTCTC 3 cut(s) 42, 378, 653
BcuI ACTAGT 1 cut(s) 1144
BfaI CTAG 4 cut(s) 885, 1145, 1193, 1334
BfmI CTRYAG 1 cut(s) 577
BfuAI ACCTGC 1 cut(s) 1240
BglI GCCNNNNNGGC 2 cut(s) 32, 709
BglII AGATCT 1 cut(s) 1027
BisI GCNGC 4 cut(s) 125, 602, 777, 804
BlnI CCTAGG 1 cut(s) 884
BlsI GCNGC 4 cut(s) 126, 603, 778, 805
Bme1390I CCNGG 1 cut(s) 28
BmgBI CACGTC 1 cut(s) 1050
BmgT120I GGNCC 1 cut(s) 914
BmiI GGNNCC 2 cut(s) 70, 448
BmrFI CCNGG 1 cut(s) 28
BmrI ACTGGG 2 cut(s) 150, 642
BmsI GCATC 4 cut(s) 106, 1143, 1200, 1222
BmtI GCTAGC 1 cut(s) 1196
BmuI ACTGGG 2 cut(s) 150, 642
BpiI GAAGAC 2 cut(s) 942, 1176
BplI GAGNNNNNCTC 2 cut(s) 1328, 1360
BpmI CTGGAG 2 cut(s) 62, 113
Bpu10I CCTNAGC 1 cut(s) 996
BpuMI CCSGG 1 cut(s) 28
BsaHI GRCGYC 1 cut(s) 35
BsaJI CCNNGG 1 cut(s) 884
BsaXI ACNNNNNCTCC 2 cut(s) 86, 116
Bsc4I CCNNNNNNNGG 2 cut(s) 633, 1059
Bse118I RCCGGY 1 cut(s) 712
Bse1I ACTGG 6 cut(s) 156, 344, 369, 637, 910, 1109
Bse3DI GCAATG 2 cut(s) 474, 1007
BseDI CCNNGG 1 cut(s) 884
BseLI CCNNNNNNNGG 2 cut(s) 633, 1059
BseMI GCAATG 2 cut(s) 474, 1007
BseMII CTCAG 3 cut(s) 522, 987, 1270
BseNI ACTGG 6 cut(s) 156, 344, 369, 637, 910, 1109
BseXI GCAGC 4 cut(s) 136, 613, 788, 790
Bsh1236I CGCG 2 cut(s) 145, 966
BshFI GGCC 5 cut(s) 133, 150, 712, 916, 1167
BsiSI CCGG 2 cut(s) 28, 713
BslI CCNNNNNNNGG 2 cut(s) 633, 1059
BsmAI GTCTC 3 cut(s) 42, 378, 653
BsmBI CGTCTC 1 cut(s) 42
BsnI GGCC 5 cut(s) 133, 150, 712, 916, 1167
Bsp143I GATC 5 cut(s) 183, 520, 1019, 1027, 1066
BspACI CCGC 1 cut(s) 240
BspANI GGCC 5 cut(s) 133, 150, 712, 916, 1167
BspCNI CTCAG 3 cut(s) 523, 988, 1269
BspFNI CGCG 2 cut(s) 145, 966
BspLI GGNNCC 2 cut(s) 70, 448
BspMAI CTGCAG 1 cut(s) 581
BspMI ACCTGC 1 cut(s) 1240
BspOI GCTAGC 1 cut(s) 1196
BspPI GGATC 2 cut(s) 1027, 1074
BspQI GCTCTTC 1 cut(s) 1305
BsrBI CCGCTC 1 cut(s) 242
BsrDI GCAATG 2 cut(s) 474, 1007
BsrFI RCCGGY 1 cut(s) 712
BsrI ACTGG 6 cut(s) 156, 344, 369, 637, 910, 1109
BssAI RCCGGY 1 cut(s) 712
BssECI CCNNGG 1 cut(s) 884
BssMI GATC 5 cut(s) 183, 520, 1019, 1027, 1066
BssNAI GTATAC 1 cut(s) 161
BssNI GRCGYC 1 cut(s) 35
BssT1I CCWWGG 1 cut(s) 884
Bst1107I GTATAC 1 cut(s) 161
Bst4CI ACNGT 2 cut(s) 410, 511
Bst6I CTCTTC 3 cut(s) 633, 831, 1305
BstACI GRCGYC 1 cut(s) 35
BstC8I GCNNGC 7 cut(s) 24, 33, 307, 714, 1043, 1194, 1317
BstDEI CTNAG 6 cut(s) 286, 531, 596, 996, 1256, 1354
BstFNI CGCG 2 cut(s) 145, 966
BstKTI GATC 5 cut(s) 186, 523, 1022, 1030, 1069
BstMAI GTCTC 3 cut(s) 42, 378, 653
BstMBI GATC 5 cut(s) 183, 520, 1019, 1027, 1066
BstMWI GCNNNNNNNGC 5 cut(s) 28, 32, 130, 709, 735
BstNSI RCATGY 3 cut(s) 1093, 1239, 1319
BstSCI CCNGG 1 cut(s) 26
BstSFI CTRYAG 1 cut(s) 577
BstUI CGCG 2 cut(s) 145, 966
BstV1I GCAGC 4 cut(s) 136, 613, 788, 790
BstV2I GAAGAC 2 cut(s) 942, 1176
BstX2I RGATCY 2 cut(s) 1027, 1066
BstYI RGATCY 2 cut(s) 1027, 1066
BstZ17I GTATAC 1 cut(s) 161
BsuRI GGCC 5 cut(s) 133, 150, 712, 916, 1167
BtrI CACGTC 1 cut(s) 1050
BtsI GCAGTG 2 cut(s) 134, 198
BtsIMutI CAGTG 3 cut(s) 134, 198, 630
BveI ACCTGC 1 cut(s) 1240
Cac8I GCNNGC 7 cut(s) 24, 33, 307, 714, 1043, 1194, 1317
Cfr10I RCCGGY 1 cut(s) 712
Cfr13I GGNCC 1 cut(s) 914
CseI GACGC 1 cut(s) 24
Csp6I GTAC 4 cut(s) 684, 732, 880, 978
CspCI CAANNNNNGTGG 2 cut(s) 661, 696
CviAII CATG 9 cut(s) 11, 476, 652, 784, 1075, 1090, 1131, 1236, 1316
CviQI GTAC 4 cut(s) 684, 732, 880, 978
DdeI CTNAG 6 cut(s) 286, 531, 596, 996, 1256, 1354
DpnI GATC 5 cut(s) 185, 522, 1021, 1029, 1068
DpnII GATC 5 cut(s) 183, 520, 1019, 1027, 1066
DraI TTTAAA 1 cut(s) 813
EaeI YGGCCR 1 cut(s) 131
Eam1104I CTCTTC 3 cut(s) 633, 831, 1305
EarI CTCTTC 3 cut(s) 633, 831, 1305
Eco130I CCWWGG 1 cut(s) 884
Eco57I CTGAAG 1 cut(s) 1170
EcoO109I RGGNCCY 1 cut(s) 914
EcoT14I CCWWGG 1 cut(s) 884
EcoT22I ATGCAT 2 cut(s) 1136, 1237
ErhI CCWWGG 1 cut(s) 884
Esp3I CGTCTC 1 cut(s) 42
FaeI CATG 9 cut(s) 14, 479, 655, 787, 1078, 1093, 1134, 1239, 1319
FalI AAGNNNNNCTT 2 cut(s) 860, 892
FatI CATG 9 cut(s) 10, 475, 651, 783, 1074, 1089, 1130, 1235, 1315
FauI CCCGC 1 cut(s) 247
FauNDI CATATG 1 cut(s) 61
FbaI TGATCA 2 cut(s) 183, 520
FblI GTMKAC 1 cut(s) 160
Fnu4HI GCNGC 4 cut(s) 125, 602, 777, 804
Fsp4HI GCNGC 4 cut(s) 125, 602, 777, 804
FspBI CTAG 4 cut(s) 885, 1145, 1193, 1334
GluI GCNGC 4 cut(s) 125, 602, 777, 804
GsuI CTGGAG 2 cut(s) 62, 113
HaeIII GGCC 5 cut(s) 133, 150, 712, 916, 1167
HapII CCGG 2 cut(s) 28, 713
HgaI GACGC 1 cut(s) 24
Hin1I GRCGYC 1 cut(s) 35
Hin1II CATG 9 cut(s) 14, 479, 655, 787, 1078, 1093, 1134, 1239, 1319
HincII GTYRAC 1 cut(s) 337
HindII GTYRAC 1 cut(s) 337
HinfI GANTC 5 cut(s) 196, 265, 742, 824, 1241
HpaII CCGG 2 cut(s) 28, 713
Hpy166II GTNNAC 5 cut(s) 161, 337, 669, 1087, 1103
Hpy188I TCNGA 5 cut(s) 73, 264, 282, 1189, 1357
Hpy188III TCNNGA 7 cut(s) 41, 245, 530, 617, 746, 1229, 1258
Hpy8I GTNNAC 5 cut(s) 161, 337, 669, 1087, 1103
HpyAV CCTTC 6 cut(s) 161, 181, 282, 844, 1072, 1178
HpyCH4III ACNGT 2 cut(s) 410, 511
HpyCH4IV ACGT 2 cut(s) 690, 1049
HpyF10VI GCNNNNNNNGC 5 cut(s) 28, 32, 130, 709, 735
HpyF3I CTNAG 6 cut(s) 286, 531, 596, 996, 1256, 1354
HpySE526I ACGT 2 cut(s) 690, 1049
Hsp92I GRCGYC 1 cut(s) 35
Hsp92II CATG 9 cut(s) 14, 479, 655, 787, 1078, 1093, 1134, 1239, 1319
KroI GCCGGC 1 cut(s) 712
KroNI GCCGGC 1 cut(s) 714
Ksp22I TGATCA 2 cut(s) 183, 520
Kzo9I GATC 5 cut(s) 183, 520, 1019, 1027, 1066
LguI GCTCTTC 1 cut(s) 1305
LmnI GCTCC 3 cut(s) 94, 216, 247
Lsp1109I GCAGC 4 cut(s) 136, 613, 788, 790
LweI GCATC 4 cut(s) 106, 1143, 1200, 1222
MaeI CTAG 4 cut(s) 885, 1145, 1193, 1334
MaeII ACGT 2 cut(s) 690, 1049
MaeIII GTNAC 3 cut(s) 193, 457, 691
MalI GATC 5 cut(s) 185, 522, 1021, 1029, 1068
MbiI CCGCTC 1 cut(s) 242
MboI GATC 5 cut(s) 183, 520, 1019, 1027, 1066
MflI RGATCY 2 cut(s) 1027, 1066
MluCI AATT 9 cut(s) 200, 346, 392, 479, 490, 591, 762, 807, 942
MluI ACGCGT 1 cut(s) 964
MlyI GAGTC 2 cut(s) 190, 736
MmeI TCCRAC 3 cut(s) 305, 565, 1044
Mph1103I ATGCAT 2 cut(s) 1136, 1237
MroNI GCCGGC 1 cut(s) 712
MseI TTAA 6 cut(s) 165, 206, 231, 501, 812, 1328
MspA1I CMGCKG 1 cut(s) 779
MspI CCGG 2 cut(s) 28, 713
MspR9I CCNGG 1 cut(s) 28
MvnI CGCG 2 cut(s) 145, 966
MwoI GCNNNNNNNGC 5 cut(s) 28, 32, 130, 709, 735
NaeI GCCGGC 1 cut(s) 714
NciI CCSGG 1 cut(s) 28
NdeI CATATG 1 cut(s) 61
NdeII GATC 5 cut(s) 183, 520, 1019, 1027, 1066
NgoMIV GCCGGC 1 cut(s) 712
NheI GCTAGC 1 cut(s) 1192
NlaIII CATG 9 cut(s) 14, 479, 655, 787, 1078, 1093, 1134, 1239, 1319
NlaIV GGNNCC 2 cut(s) 70, 448
NmuCI GTSAC 1 cut(s) 193
NsiI ATGCAT 2 cut(s) 1136, 1237
NspI RCATGY 3 cut(s) 1093, 1239, 1319
PaeI GCATGC 1 cut(s) 1319
PciI ACATGT 1 cut(s) 1089
PciSI GCTCTTC 1 cut(s) 1305
PdiI GCCGGC 1 cut(s) 714
PfeI GAWTC 3 cut(s) 265, 824, 1241
PkrI GCNGC 4 cut(s) 126, 603, 778, 805
PleI GAGTC 2 cut(s) 190, 736
PpsI GAGTC 2 cut(s) 190, 736
PscI ACATGT 1 cut(s) 1089
PshBI ATTAAT 1 cut(s) 1328
PspN4I GGNNCC 2 cut(s) 70, 448
PspPI GGNCC 1 cut(s) 914
PsrI GAACNNNNNNTAC 2 cut(s) 243, 275
PstI CTGCAG 1 cut(s) 581
PsuI RGATCY 2 cut(s) 1027, 1066
PvuII CAGCTG 1 cut(s) 779
RsaI GTAC 4 cut(s) 685, 733, 881, 979
RsaNI GTAC 4 cut(s) 684, 732, 880, 978
SapI GCTCTTC 1 cut(s) 1305
SaqAI TTAA 6 cut(s) 165, 206, 231, 501, 812, 1328
SatI GCNGC 4 cut(s) 125, 602, 777, 804
Sau3AI GATC 5 cut(s) 183, 520, 1019, 1027, 1066
Sau96I GGNCC 1 cut(s) 914
SchI GAGTC 2 cut(s) 190, 736
ScrFI CCNGG 1 cut(s) 28
SfaNI GCATC 4 cut(s) 106, 1143, 1200, 1222
SfcI CTRYAG 1 cut(s) 577
SpeI ACTAGT 1 cut(s) 1144
SphI GCATGC 1 cut(s) 1319
Sse9I AATT 9 cut(s) 200, 346, 392, 479, 490, 591, 762, 807, 942
SsiI CCGC 1 cut(s) 240
SspMI CTAG 4 cut(s) 885, 1145, 1193, 1334
StyD4I CCNGG 1 cut(s) 26
StyI CCWWGG 1 cut(s) 884
TaaI ACNGT 2 cut(s) 410, 511
TaiI ACGT 2 cut(s) 693, 1052
TaqI TCGA 1 cut(s) 555
TasI AATT 9 cut(s) 200, 346, 392, 479, 490, 591, 762, 807, 942
TatI WGTACW 1 cut(s) 731
TfiI GAWTC 3 cut(s) 265, 824, 1241
Tru1I TTAA 6 cut(s) 165, 206, 231, 501, 812, 1328
Tru9I TTAA 6 cut(s) 165, 206, 231, 501, 812, 1328
TscAI CASTG 3 cut(s) 134, 198, 637
TseFI GTSAC 1 cut(s) 193
TseI GCWGC 4 cut(s) 124, 601, 776, 803
Tsp45I GTSAC 1 cut(s) 193
TspDTI ATGAA 9 cut(s) 492, 668, 765, 831, 948, 1091, 1167, 1215, 1272
TspRI CASTG 3 cut(s) 134, 198, 637
VspI ATTAAT 1 cut(s) 1328
XapI RAATTY 2 cut(s) 591, 762
XceI RCATGY 3 cut(s) 1093, 1239, 1319
XcmI CCANNNNNNNNNTGG 1 cut(s) 1081
XmaJI CCTAGG 1 cut(s) 884
XmiI GTMKAC 1 cut(s) 160
XspI CTAG 4 cut(s) 885, 1145, 1193, 1334
Zsp2I ATGCAT 2 cut(s) 1136, 1237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.