Rroxscaffold_1G00051930

Glycosyl transferases group 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0008696
Physical Location & Seq
Reverse (-)
72708037 .. 72713911
5875 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00051930.1

Sequence Viewer

Length: 1470 bp
ATGGCCAGAAAAGGAGAGAAGAGAGGGAGGGCTTGTGTGGTGGTATTAGGTGACATTGGGCGCAGCCCTCGTATGCAATACCATGCTCTTTCGCTTGCCCGTCAGGCATCTCTGGAAGTAGATATTGTTGCATATGGAGGTTCCGAACCGCATTCTGCTGTGTTGGAGCATCAATCTATTCACATACACAAAATGCCGCAGTGGCCAGCATTCCCCCGTGGTCTACCCAGCATACTTAAACCCTTTGTGCTTCTGCTGAAGCCAGTGGTTCAATTTATTGTGCTCCTTTGGTATCTTTGCGTTAAGATATCTGCTCCTGATGTTTTTCTAGTTCAGAATCCACCTTCTGTTCCAACCTTAGTCGCTGTAAAATGGGCAAGCTGGCTTAGGCAATCTGCATTTATAGTTGATTGGCATAATTTTGGATATACTTTACTGGCATTGTCTCTTGGAAGAAGTAGTCGATTTGTGGCAATATACCGTTGGGTAGAGTGGCAATTTGGGAGGATGGCAGATGGTTCACTATGTGTAACGAGGGCAATGCAACATGAATTGGCTCAAAATTGGGGAATTAAAGCAACAGTTCTATATGATCAGCCTCCTGAGTTTTTCCATCCCGCTTCACTTGAAGAACAGCATAAGTTGTTCTATAGATTAGATAAAAATCTGAGGCAGCCACTCGGTGTTGAAGATTGCACAAGCACTGGAATAGGGGAAGTGGTGAATAAGGACATGCAGGAGACTCTGTTTACCACCCTGGTTGGCGCTGACATTGCTTCGAAGCCAAACCGGCCGGCACTGATAGTGAGCAGTACAAGCTGGACTCCTGATGAAGATTTTGGCATTCTCCTGGAAGCAGCTGTCATGTATGACAGGCGCGTTGCTGCTATTTTAAATGAAGACGATTCTAAGAAAGAAGAGGTTCTTTGGAAGGAAATGCAAAGTGGGAAGCAATACTTGTATCCAAGACTGTTATTTGTCATTACAGGTAAAGGGCCTGAAAAAGAAAAATATGAAGAAAAGATTAGAAGATTAAAACTCAAACGGGTGGCATTTCGTACTGCGTGGCTATCAGCTGAAGACTATCCACTGCTTCTTGGATCAGCAGATCTTGGTGTGTGCTTACACACTTCCTCATCAGGGTTGGATCTTCCCATGAAGGTGGTGGACATGTTTGGTTGTGGACTGCCGGTTTGCGCTGTTTCCTACTCATGCATCAAGGAACTAGTGCAAGTGGAGAAAAATGGTCTTCTATTTTCATCATCTTCAGAGCTAGCTGATGAACTTTTGATGCTGTTTAAGGGTTTTCCAGATGCTTGTGATTCATTGAAGGTTCTGAGGAATGGTGCACTGGACATGTCTTCGTCTGTGAAGTGGGCCACTGAGTGGGAAGAGCATGCTAAAGGATTAATCTTAGAGGCAAGTTTACTCTCTGCATCCAAATCAGTCCTGCTTATCATCTATATGTAA

Protein Analysis

489

Amino Acids

54.86

Weight (kDa)

7.15

Isoelectric Point (pI)

44.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_trans_4_4 PF13579 34 - 193 5.1e-09 Glycosyl transferase 4-like domain
Glyco_transf_4 PF13439 35 - 193 2.5e-07 Glycosyltransferase Family 4
Glyco_trans_1_4 PF13692 307 - 433 8.7e-11 Glycosyl transferases group 1
Glycos_transf_1 PF00534 309 - 429 1e-09 Glycosyl transferases group 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1386
AccI GTMKAC 1 cut(s) 223
AccII CGCG 1 cut(s) 879
AciI CCGC 3 cut(s) 149, 197, 618
AclWI GGATC 2 cut(s) 1108, 1155
AcoI YGGCCR 3 cut(s) 3, 203, 791
AcuI CTGAAG 3 cut(s) 278, 1098, 1251
AdeI CACNNNGTG 3 cut(s) 527, 683, 1386
AfaI GTAC 2 cut(s) 814, 1060
AfiI CCNNNNNNNGG 2 cut(s) 1140, 1386
AflIII ACRYGT 2 cut(s) 1170, 1356
AgsI TTSAA 4 cut(s) 272, 629, 689, 1330
AhlI ACTAGT 1 cut(s) 1225
AjnI CCWGG 2 cut(s) 756, 849
AluBI AGCT 6 cut(s) 381, 819, 860, 1076, 1273, 1277
AluI AGCT 6 cut(s) 381, 819, 860, 1076, 1273, 1277
Alw21I GWGCWC 2 cut(s) 285, 1351
Alw26I GTCTC 2 cut(s) 450, 734
Alw44I GTGCAC 1 cut(s) 1347
AlwI GGATC 2 cut(s) 1108, 1155
AoxI GGCC 5 cut(s) 3, 203, 791, 995, 1377
ApaLI GTGCAC 1 cut(s) 1347
ApeKI GCWGC 4 cut(s) 63, 673, 857, 884
ArsI GACNNNNNNTTYG 2 cut(s) 1176, 1208
AseI ATTAAT 1 cut(s) 1409
Asp700I GAANNNNTTC 1 cut(s) 921
AspLEI GCGC 4 cut(s) 63, 767, 879, 1199
AspS9I GGNCC 2 cut(s) 995, 1377
AsuHPI GGTGA 2 cut(s) 62, 733
AsuII TTCGAA 1 cut(s) 779
AsuNHI GCTAGC 1 cut(s) 1273
BaeGI GKGCMC 1 cut(s) 1351
BalI TGGCCA 2 cut(s) 5, 205
BbsI GAAGAC 4 cut(s) 906, 1086, 1241, 1353
Bbv12I GWGCWC 2 cut(s) 285, 1351
BbvI GCAGC 4 cut(s) 75, 685, 869, 871
BccI CCATC 3 cut(s) 502, 509, 621
BcgI CGANNNNNNTGC 2 cut(s) 523, 557
BciT130I CCWGG 2 cut(s) 758, 851
BciVI GTATCC 1 cut(s) 972
BclI TGATCA 1 cut(s) 592
BcoDI GTCTC 2 cut(s) 450, 734
BcuI ACTAGT 1 cut(s) 1225
BfaI CTAG 3 cut(s) 329, 1226, 1274
BfmI CTRYAG 1 cut(s) 649
BfoI RGCGCY 1 cut(s) 768
BfuI GTATCC 1 cut(s) 972
BglI GCCNNNNNGGC 3 cut(s) 104, 202, 790
BglII AGATCT 1 cut(s) 1108
BisI GCNGC 5 cut(s) 64, 197, 674, 858, 885
BlsI GCNGC 5 cut(s) 65, 198, 675, 859, 886
Bme1390I CCNGG 2 cut(s) 758, 851
BmgT120I GGNCC 2 cut(s) 995, 1377
BmiI GGNNCC 1 cut(s) 142
BmrFI CCNGG 2 cut(s) 758, 851
BmsI GCATC 6 cut(s) 116, 178, 1224, 1281, 1303, 1445
BmtI GCTAGC 1 cut(s) 1277
BpiI GAAGAC 4 cut(s) 906, 1086, 1241, 1353
Bpu10I CCTNAGC 1 cut(s) 386
Bpu14I TTCGAA 1 cut(s) 779
BsaBI GATNNNNATC 1 cut(s) 663
BsaJI CCNNGG 2 cut(s) 217, 756
BsaXI ACNNNNNCTCC 4 cut(s) 19, 49, 731, 761
Bsc4I CCNNNNNNNGG 2 cut(s) 1140, 1386
Bse118I RCCGGY 3 cut(s) 789, 793, 1189
Bse1I ACTGG 4 cut(s) 263, 441, 709, 1356
Bse3DI GCAATG 2 cut(s) 546, 771
Bse8I GATNNNNATC 1 cut(s) 663
BseBI CCWGG 2 cut(s) 758, 851
BseDI CCNNGG 2 cut(s) 217, 756
BseGI GGATG 3 cut(s) 513, 613, 1436
BseJI GATNNNNATC 1 cut(s) 663
BseLI CCNNNNNNNGG 2 cut(s) 1140, 1386
BseMI GCAATG 2 cut(s) 546, 771
BseMII CTCAG 4 cut(s) 594, 659, 1328, 1374
BseNI ACTGG 4 cut(s) 263, 441, 709, 1356
BseSI GKGCMC 1 cut(s) 1351
BseX3I CGGCCG 1 cut(s) 791
BseXI GCAGC 4 cut(s) 75, 685, 869, 871
BseYI CCCAGC 1 cut(s) 227
Bsh1236I CGCG 1 cut(s) 879
Bsh1285I CGRYCG 1 cut(s) 794
BshFI GGCC 5 cut(s) 5, 205, 793, 997, 1379
BsiEI CGRYCG 1 cut(s) 794
BsiHKAI GWGCWC 2 cut(s) 285, 1351
BsiSI CCGG 3 cut(s) 790, 794, 1190
BslI CCNNNNNNNGG 2 cut(s) 1140, 1386
BsmAI GTCTC 2 cut(s) 450, 734
BsmI GAATGC 3 cut(s) 151, 209, 843
BsnI GGCC 5 cut(s) 5, 205, 793, 997, 1379
Bsp119I TTCGAA 1 cut(s) 779
Bsp1286I GDGCHC 2 cut(s) 285, 1351
Bsp143I GATC 4 cut(s) 592, 1100, 1108, 1147
BspACI CCGC 3 cut(s) 149, 197, 618
BspANI GGCC 5 cut(s) 5, 205, 793, 997, 1379
BspCNI CTCAG 4 cut(s) 595, 660, 1329, 1375
BspFNI CGCG 1 cut(s) 879
BspLI GGNNCC 1 cut(s) 142
BspOI GCTAGC 1 cut(s) 1277
BspPI GGATC 2 cut(s) 1108, 1155
BspQI GCTCTTC 1 cut(s) 1386
BspT104I TTCGAA 1 cut(s) 779
BsrDI GCAATG 2 cut(s) 546, 771
BsrFI RCCGGY 3 cut(s) 789, 793, 1189
BsrI ACTGG 4 cut(s) 263, 441, 709, 1356
BssAI RCCGGY 3 cut(s) 789, 793, 1189
BssECI CCNNGG 2 cut(s) 217, 756
BssMI GATC 4 cut(s) 592, 1100, 1108, 1147
Bst2UI CCWGG 2 cut(s) 758, 851
Bst4CI ACNGT 3 cut(s) 482, 583, 972
Bst6I CTCTTC 3 cut(s) 14, 912, 1386
BstBI TTCGAA 1 cut(s) 779
BstC8I GCNNGC 7 cut(s) 96, 207, 379, 383, 795, 1275, 1398
BstDEI CTNAG 8 cut(s) 358, 386, 603, 668, 909, 1337, 1383, 1414
BstDSI CCRYGG 1 cut(s) 217
BstF5I GGATG 3 cut(s) 513, 613, 1436
BstFNI CGCG 1 cut(s) 879
BstH2I RGCGCY 1 cut(s) 768
BstHHI GCGC 4 cut(s) 63, 767, 879, 1199
BstKTI GATC 4 cut(s) 595, 1103, 1111, 1150
BstMAI GTCTC 2 cut(s) 450, 734
BstMBI GATC 4 cut(s) 592, 1100, 1108, 1147
BstMCI CGRYCG 1 cut(s) 794
BstMWI GCNNNNNNNGC 5 cut(s) 104, 202, 773, 790, 816
BstNI CCWGG 2 cut(s) 758, 851
BstNSI RCATGY 4 cut(s) 736, 1174, 1360, 1400
BstSCI CCNGG 2 cut(s) 756, 849
BstSFI CTRYAG 1 cut(s) 649
BstSLI GKGCMC 1 cut(s) 1351
BstUI CGCG 1 cut(s) 879
BstV1I GCAGC 4 cut(s) 75, 685, 869, 871
BstV2I GAAGAC 4 cut(s) 906, 1086, 1241, 1353
BstX2I RGATCY 2 cut(s) 1108, 1147
BstXI CCANNNNNNTGG 1 cut(s) 1162
BstYI RGATCY 2 cut(s) 1108, 1147
BstZI CGGCCG 1 cut(s) 791
BsuI GTATCC 1 cut(s) 972
BsuRI GGCC 5 cut(s) 5, 205, 793, 997, 1379
BtgI CCRYGG 1 cut(s) 217
BtsCI GGATG 3 cut(s) 513, 613, 1436
BtsI GCAGTG 2 cut(s) 206, 1088
BtsIMutI CAGTG 7 cut(s) 206, 270, 702, 797, 1088, 1349, 1380
Cac8I GCNNGC 7 cut(s) 96, 207, 379, 383, 795, 1275, 1398
CfoI GCGC 4 cut(s) 63, 767, 879, 1199
Cfr10I RCCGGY 3 cut(s) 789, 793, 1189
Cfr13I GGNCC 2 cut(s) 995, 1377
Csp6I GTAC 2 cut(s) 813, 1059
CspCI CAANNNNNGTGG 2 cut(s) 742, 777
CviAII CATG 9 cut(s) 83, 548, 733, 865, 1156, 1171, 1212, 1357, 1397
CviQI GTAC 2 cut(s) 813, 1059
DdeI CTNAG 8 cut(s) 358, 386, 603, 668, 909, 1337, 1383, 1414
DpnI GATC 4 cut(s) 594, 1102, 1110, 1149
DpnII GATC 4 cut(s) 592, 1100, 1108, 1147
DraI TTTAAA 1 cut(s) 894
DraIII CACNNNGTG 3 cut(s) 527, 683, 1386
EaeI YGGCCR 3 cut(s) 3, 203, 791
EagI CGGCCG 1 cut(s) 791
Eam1104I CTCTTC 3 cut(s) 14, 912, 1386
EarI CTCTTC 3 cut(s) 14, 912, 1386
EclXI CGGCCG 1 cut(s) 791
Eco32I GATATC 1 cut(s) 309
Eco52I CGGCCG 1 cut(s) 791
Eco57I CTGAAG 3 cut(s) 278, 1098, 1251
EcoO109I RGGNCCY 1 cut(s) 995
EcoRII CCWGG 2 cut(s) 756, 849
EcoRV GATATC 1 cut(s) 309
EcoT22I ATGCAT 1 cut(s) 1217
FaeI CATG 9 cut(s) 86, 551, 736, 868, 1159, 1174, 1215, 1360, 1400
FalI AAGNNNNNCTT 4 cut(s) 909, 941, 941, 973
FatI CATG 9 cut(s) 82, 547, 732, 864, 1155, 1170, 1211, 1356, 1396
FauI CCCGC 1 cut(s) 625
FauNDI CATATG 1 cut(s) 133
FbaI TGATCA 1 cut(s) 592
FblI GTMKAC 1 cut(s) 223
Fnu4HI GCNGC 5 cut(s) 64, 197, 674, 858, 885
FokI GGATG 3 cut(s) 520, 600, 1423
Fsp4HI GCNGC 5 cut(s) 64, 197, 674, 858, 885
FspBI CTAG 3 cut(s) 329, 1226, 1274
GlaI GCGC 4 cut(s) 62, 766, 878, 1198
GluI GCNGC 5 cut(s) 64, 197, 674, 858, 885
GsaI CCCAGC 1 cut(s) 231
HaeII RGCGCY 1 cut(s) 768
HaeIII GGCC 5 cut(s) 5, 205, 793, 997, 1379
HapII CCGG 3 cut(s) 790, 794, 1190
HhaI GCGC 4 cut(s) 63, 767, 879, 1199
Hin1II CATG 9 cut(s) 86, 551, 736, 868, 1159, 1174, 1215, 1360, 1400
Hin6I GCGC 4 cut(s) 61, 765, 877, 1197
HinP1I GCGC 4 cut(s) 61, 765, 877, 1197
HinfI GANTC 5 cut(s) 337, 742, 823, 905, 1322
HpaII CCGG 3 cut(s) 790, 794, 1190
HphI GGTGA 2 cut(s) 62, 733
Hpy166II GTNNAC 7 cut(s) 224, 521, 750, 1168, 1184, 1349, 1427
Hpy188I TCNGA 5 cut(s) 145, 336, 669, 1270, 1338
Hpy188III TCNNGA 5 cut(s) 113, 317, 602, 827, 1310
Hpy8I GTNNAC 7 cut(s) 224, 521, 750, 1168, 1184, 1349, 1427
HpyAV CCTTC 4 cut(s) 354, 925, 1153, 1324
HpyCH4III ACNGT 3 cut(s) 482, 583, 972
HpyF10VI GCNNNNNNNGC 5 cut(s) 104, 202, 773, 790, 816
HpyF3I CTNAG 8 cut(s) 358, 386, 603, 668, 909, 1337, 1383, 1414
Hsp92II CATG 9 cut(s) 86, 551, 736, 868, 1159, 1174, 1215, 1360, 1400
HspAI GCGC 4 cut(s) 61, 765, 877, 1197
KroI GCCGGC 1 cut(s) 793
KroNI GCCGGC 1 cut(s) 795
Ksp22I TGATCA 1 cut(s) 592
Kzo9I GATC 4 cut(s) 592, 1100, 1108, 1147
LguI GCTCTTC 1 cut(s) 1386
LmnI GCTCC 3 cut(s) 166, 288, 319
Lsp1109I GCAGC 4 cut(s) 75, 685, 869, 871
LweI GCATC 6 cut(s) 116, 178, 1224, 1281, 1303, 1445
MaeI CTAG 3 cut(s) 329, 1226, 1274
MaeIII GTNAC 2 cut(s) 50, 529
MalI GATC 4 cut(s) 594, 1102, 1110, 1149
MboI GATC 4 cut(s) 592, 1100, 1108, 1147
MflI RGATCY 2 cut(s) 1108, 1147
MhlI GDGCHC 2 cut(s) 285, 1351
MlsI TGGCCA 2 cut(s) 5, 205
MluCI AATT 6 cut(s) 272, 418, 497, 551, 562, 570
MluNI TGGCCA 2 cut(s) 5, 205
MlyI GAGTC 2 cut(s) 736, 817
MmeI TCCRAC 3 cut(s) 144, 377, 1125
Mox20I TGGCCA 2 cut(s) 5, 205
Mph1103I ATGCAT 1 cut(s) 1217
MroNI GCCGGC 1 cut(s) 793
MroXI GAANNNNTTC 1 cut(s) 921
MscI TGGCCA 2 cut(s) 5, 205
MseI TTAA 7 cut(s) 237, 303, 573, 893, 1034, 1299, 1409
MslI CAYNNNNRTG 2 cut(s) 1160, 1463
Msp20I TGGCCA 2 cut(s) 5, 205
MspA1I CMGCKG 2 cut(s) 860, 1076
MspI CCGG 3 cut(s) 790, 794, 1190
MspR9I CCNGG 2 cut(s) 758, 851
Mva1269I GAATGC 3 cut(s) 151, 209, 843
MvaI CCWGG 2 cut(s) 758, 851
MvnI CGCG 1 cut(s) 879
MwoI GCNNNNNNNGC 5 cut(s) 104, 202, 773, 790, 816
NaeI GCCGGC 1 cut(s) 795
NdeI CATATG 1 cut(s) 133
NdeII GATC 4 cut(s) 592, 1100, 1108, 1147
NgoMIV GCCGGC 1 cut(s) 793
NheI GCTAGC 1 cut(s) 1273
NlaIII CATG 9 cut(s) 86, 551, 736, 868, 1159, 1174, 1215, 1360, 1400
NlaIV GGNNCC 1 cut(s) 142
NmuCI GTSAC 1 cut(s) 50
NsiI ATGCAT 1 cut(s) 1217
NspI RCATGY 4 cut(s) 736, 1174, 1360, 1400
NspV TTCGAA 1 cut(s) 779
PaeI GCATGC 1 cut(s) 1400
PciI ACATGT 2 cut(s) 1170, 1356
PciSI GCTCTTC 1 cut(s) 1386
PctI GAATGC 3 cut(s) 151, 209, 843
PdiI GCCGGC 1 cut(s) 795
PdmI GAANNNNTTC 1 cut(s) 921
PfeI GAWTC 3 cut(s) 337, 905, 1322
PflMI CCANNNNNTGG 1 cut(s) 1386
PfoI TCCNGGA 1 cut(s) 849
PkrI GCNGC 5 cut(s) 65, 198, 675, 859, 886
PleI GAGTC 2 cut(s) 736, 817
PpsI GAGTC 2 cut(s) 736, 817
PscI ACATGT 2 cut(s) 1170, 1356
PshBI ATTAAT 1 cut(s) 1409
Psp6I CCWGG 2 cut(s) 756, 849
PspFI CCCAGC 1 cut(s) 227
PspGI CCWGG 2 cut(s) 756, 849
PspN4I GGNNCC 1 cut(s) 142
PspPI GGNCC 2 cut(s) 995, 1377
PsuI RGATCY 2 cut(s) 1108, 1147
PvuII CAGCTG 2 cut(s) 860, 1076
RsaI GTAC 2 cut(s) 814, 1060
RsaNI GTAC 2 cut(s) 813, 1059
RseI CAYNNNNRTG 2 cut(s) 1160, 1463
SapI GCTCTTC 1 cut(s) 1386
SaqAI TTAA 7 cut(s) 237, 303, 573, 893, 1034, 1299, 1409
SatI GCNGC 5 cut(s) 64, 197, 674, 858, 885
Sau3AI GATC 4 cut(s) 592, 1100, 1108, 1147
Sau96I GGNCC 2 cut(s) 995, 1377
SchI GAGTC 2 cut(s) 736, 817
ScrFI CCNGG 2 cut(s) 758, 851
SduI GDGCHC 2 cut(s) 285, 1351
SfaNI GCATC 6 cut(s) 116, 178, 1224, 1281, 1303, 1445
SfcI CTRYAG 1 cut(s) 649
SfuI TTCGAA 1 cut(s) 779
SmiMI CAYNNNNRTG 2 cut(s) 1160, 1463
SpeI ACTAGT 1 cut(s) 1225
SphI GCATGC 1 cut(s) 1400
Sse9I AATT 6 cut(s) 272, 418, 497, 551, 562, 570
SsiI CCGC 3 cut(s) 149, 197, 618
SspMI CTAG 3 cut(s) 329, 1226, 1274
StyD4I CCNGG 2 cut(s) 756, 849
TaaI ACNGT 3 cut(s) 482, 583, 972
TaqI TCGA 2 cut(s) 463, 779
TasI AATT 6 cut(s) 272, 418, 497, 551, 562, 570
TatI WGTACW 1 cut(s) 812
TauI GCSGC 1 cut(s) 199
TfiI GAWTC 3 cut(s) 337, 905, 1322
Tru1I TTAA 7 cut(s) 237, 303, 573, 893, 1034, 1299, 1409
Tru9I TTAA 7 cut(s) 237, 303, 573, 893, 1034, 1299, 1409
TscAI CASTG 7 cut(s) 206, 270, 709, 804, 1095, 1356, 1387
TseFI GTSAC 1 cut(s) 50
TseI GCWGC 4 cut(s) 63, 673, 857, 884
Tsp45I GTSAC 1 cut(s) 50
TspDTI ATGAA 8 cut(s) 564, 846, 912, 1029, 1172, 1248, 1296, 1314
TspRI CASTG 7 cut(s) 206, 270, 709, 804, 1095, 1356, 1387
Van91I CCANNNNNTGG 1 cut(s) 1386
VneI GTGCAC 1 cut(s) 1347
VspI ATTAAT 1 cut(s) 1409
XceI RCATGY 4 cut(s) 736, 1174, 1360, 1400
XcmI CCANNNNNNNNNTGG 1 cut(s) 1162
XmiI GTMKAC 1 cut(s) 223
XmnI GAANNNNTTC 1 cut(s) 921
XspI CTAG 3 cut(s) 329, 1226, 1274
Zsp2I ATGCAT 1 cut(s) 1217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.