MD10G1197700.v1.1

Heat stress transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008306
Physical Location & Seq
Forward (+)
29592455 .. 29596135
3681 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1197700.v1.1.491

Sequence Viewer

Length: 1527 bp
ATGCAAACAACCATGGAGAGAGTCGAAGACGCGGCGTCGGTGTTCAACTCCGCCAACACTCCTCCTCCGCCGTTTCTGAACAAAACCTACGACATGGTGGATGACCCCTCCACCGACGCCGTCGTCTCCTGGAGCAAAAGCAACAACAGCTTCGTCGTCTGGAATGTCCCCGAGTTCTCCAGGGACCTCCTGCCCAAGTATTTCAAGCACAACAACTTCTCTAGCTTCGTCAGGCAGTTGAATACTTATGGTTTTAGAAAGGTTGATCCAGACCGATGGGAATTTGCAAATGAAGGGTTTCTAAGAGGCCAAAAACACCTTTTGAAGACCGTAAGCAGGCGAAAACCAGCTCATGTACAGAATCATCAACAACAACCACCTCAAGTGCAGAGCTCTCAGGTTGGGGCATGTGTAGAGGTGGGAAATCCTGGGCTAGAGGAAGAGGTCGAAAGACTTAAAAGAGATAAGAACAGTCTTATGCAGGAACTTGTTAGGCTGAGGCAGCAACAGCAAGCAACAGATAATCAGTTGCATAATGTTGGGCAACGTATGCAGGGAATGGAGCAACGGCAGCAGCAAATGATGTCTTTCCTGGCAAAGGCTATGCATAGTCCCGGATTTTTATCCCAGCTTGTACAGCATCAAAATGAAAACAATAGGCTCATCACTGGAAGCAATAAGAAAAGGAGACTCCCAAGGCAAGACGATGAAATTTTGGTTGGGAACCTTGGCAGCAAAGTTCTTGATGGACAAATGGTGAAGTACCAGCCTTCAATGAATGAAGCAGCAAAAGCCATGCTGCGGCAGATCTTGAAGATGAATACATCTCCTAAGCTGGAACCATCAATGATCAATCCTGATGCTTTCCTAATTGATAATGTTCCTTCCAATGCAACAGATACTAGGAACACCTCTAGTCGGATTTTGGGGGTGACCCTTTCAGAGGTTCCACCAATTTCTGCAGAGTGCGATATGCCCAACGAGTCTAGATTTCCCGTCAGTTGTCATTCTACAGGCATTTCTGAGGTCCAGTCTTCCCCATTCGCGGTTTCCAACTCTGTTAAAGAAACTCAAGTTTTGGAAGAGAACATGCTTAATTTTCAACAAGATCCAGTTATCCCTGAACTAACTCGAATGCAAGGTATTGTTCCACAAAGCAATGTGGAAATCCCCAATGCAAACTTCATCAACTCAGAGACTGGGAATGCAGGATACATGGGCATGTCAACAGGTTTGGATGGCAGACTGCCTACAGATATTGACCCGTTTTCTCCTGAGCCTGATGCAGATGCGTTGCCAGATGGGGTCTCTAGCCTTCCAAGCATTAATGATATCTTCTGGGAACAATTTCTTACAGCGAGCCCACTGCCTGGTGACACAGACGAAATTAGTTTGAGTTCTGATGATGGAGTGACCGTGAATCAGGACTTGCAGTTGGGGAAGGATAATGGATGGGACAAGAGTCAACATATGAATCACATCACTGAACAAATGCAGCTTCTCGCATCAGGGAGCAGAATTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSF_DNA-bind

Protein Analysis

509

Amino Acids

56.77

Weight (kDa)

5.15

Isoelectric Point (pI)

56.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 25 - 114 8.1e-30 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 122
AccB7I CCANNNNNTGG 1 cut(s) 1370
AccII CGCG 2 cut(s) 32, 1046
AciI CCGC 5 cut(s) 32, 51, 68, 802, 1046
AclWI GGATC 2 cut(s) 260, 1103
AcsI RAATTY 2 cut(s) 281, 711
AcyI GRCGYC 2 cut(s) 35, 117
AfaI GTAC 3 cut(s) 357, 636, 764
AfiI CCNNNNNNNGG 7 cut(s) 336, 598, 801, 918, 943, 1045, 1370
AgsI TTSAA 7 cut(s) 46, 205, 241, 325, 774, 814, 1103
AhdI GACNNNNNGTC 1 cut(s) 34
AjnI CCWGG 5 cut(s) 128, 179, 427, 591, 1369
AjuI GAANNNNNNNTTGG 4 cut(s) 702, 734, 1166, 1198
AluBI AGCT 7 cut(s) 150, 225, 350, 393, 631, 835, 1498
AluI AGCT 7 cut(s) 150, 225, 350, 393, 631, 835, 1498
Alw21I GWGCWC 1 cut(s) 395
Alw26I GTCTC 4 cut(s) 130, 682, 1190, 1312
AlwI GGATC 2 cut(s) 260, 1103
AlwNI CAGNNNCTG 1 cut(s) 1199
Ama87I CYCGRG 1 cut(s) 170
AoxI GGCC 1 cut(s) 307
ApeKI GCWGC 7 cut(s) 502, 571, 574, 732, 785, 799, 1495
ApoI RAATTY 2 cut(s) 281, 711
AseI ATTAAT 1 cut(s) 1326
Asp700I GAANNNNTTC 2 cut(s) 297, 1347
AspS9I GGNCC 2 cut(s) 184, 1027
AsuC2I CCSGG 1 cut(s) 615
AsuHPI GGTGA 3 cut(s) 769, 943, 1385
AvaI CYCGRG 1 cut(s) 170
AvaII GGWCC 2 cut(s) 184, 1027
BanII GRGCYC 2 cut(s) 395, 1364
BbsI GAAGAC 3 cut(s) 33, 332, 1026
Bbv12I GWGCWC 1 cut(s) 395
BbvCI CCTCAGC 1 cut(s) 497
BbvI GCAGC 7 cut(s) 514, 583, 586, 744, 786, 797, 1507
BccI CCATC 7 cut(s) 270, 740, 850, 1232, 1295, 1400, 1446
BceAI ACGGC 3 cut(s) 55, 104, 584
BcgI CGANNNNNNTGC 2 cut(s) 1348, 1382
BciT130I CCWGG 5 cut(s) 130, 181, 429, 593, 1371
BciVI GTATCC 1 cut(s) 1205
BclI TGATCA 1 cut(s) 849
BcnI CCSGG 1 cut(s) 615
BcoDI GTCTC 4 cut(s) 130, 682, 1190, 1312
BfaI CTAG 6 cut(s) 222, 434, 903, 915, 987, 1311
BfmI CTRYAG 3 cut(s) 960, 1011, 1251
BfuI GTATCC 1 cut(s) 1205
BglII AGATCT 1 cut(s) 807
BisI GCNGC 9 cut(s) 33, 503, 572, 575, 733, 786, 800, 803, 1496
BlsI GCNGC 9 cut(s) 34, 504, 573, 576, 734, 787, 801, 804, 1497
Bme1390I CCNGG 6 cut(s) 130, 181, 429, 593, 615, 1371
Bme18I GGWCC 2 cut(s) 184, 1027
BmeRI GACNNNNNGTC 1 cut(s) 34
BmeT110I CYCGRG 1 cut(s) 170
BmgT120I GGNCC 2 cut(s) 184, 1027
BmiI GGNNCC 4 cut(s) 185, 725, 840, 948
BmrFI CCNGG 6 cut(s) 130, 181, 429, 593, 615, 1371
BmrI ACTGGG 1 cut(s) 1209
BmsI GCATC 5 cut(s) 649, 850, 1273, 1279, 1514
BmuI ACTGGG 1 cut(s) 1209
BoxI GACNNNNGTC 1 cut(s) 1461
BpiI GAAGAC 3 cut(s) 33, 332, 1026
BpmI CTGGAG 2 cut(s) 151, 163
Bpu10I CCTNAGC 3 cut(s) 497, 831, 1275
BpuEI CTTGAG 2 cut(s) 366, 1056
BpuMI CCSGG 1 cut(s) 615
BsaBI GATNNNNATC 1 cut(s) 622
BsaHI GRCGYC 2 cut(s) 35, 117
BsaI GGTCTC 1 cut(s) 1312
BsaJI CCNNGG 5 cut(s) 12, 180, 428, 695, 727
BsaXI ACNNNNNCTCC 4 cut(s) 49, 79, 1401, 1431
Bsc4I CCNNNNNNNGG 7 cut(s) 336, 598, 801, 918, 943, 1045, 1370
Bse1I ACTGG 4 cut(s) 673, 1030, 1112, 1204
Bse3DI GCAATG 1 cut(s) 1165
Bse8I GATNNNNATC 1 cut(s) 622
BseBI CCWGG 5 cut(s) 130, 181, 429, 593, 1371
BseDI CCNNGG 5 cut(s) 12, 180, 428, 695, 727
BseGI GGATG 3 cut(s) 106, 1243, 1457
BseJI GATNNNNATC 1 cut(s) 622
BseLI CCNNNNNNNGG 7 cut(s) 336, 598, 801, 918, 943, 1045, 1370
BseMI GCAATG 1 cut(s) 1165
BseMII CTCAG 5 cut(s) 410, 488, 1014, 1206, 1266
BseNI ACTGG 4 cut(s) 673, 1030, 1112, 1204
BseRI GAGGAG 2 cut(s) 51, 54
BseXI GCAGC 7 cut(s) 514, 583, 586, 744, 786, 797, 1507
BseYI CCCAGC 1 cut(s) 627
BsgI GTGCAG 1 cut(s) 407
Bsh1236I CGCG 2 cut(s) 32, 1046
BshFI GGCC 1 cut(s) 309
BsiHKAI GWGCWC 1 cut(s) 395
BsiHKCI CYCGRG 1 cut(s) 170
BsiSI CCGG 1 cut(s) 615
BslFI GGGAC 4 cut(s) 152, 197, 597, 1469
BslI CCNNNNNNNGG 7 cut(s) 336, 598, 801, 918, 943, 1045, 1370
BsmAI GTCTC 4 cut(s) 130, 682, 1190, 1312
BsmBI CGTCTC 1 cut(s) 130
BsmFI GGGAC 4 cut(s) 152, 197, 597, 1469
BsmI GAATGC 2 cut(s) 1140, 1210
BsnI GGCC 1 cut(s) 309
Bso31I GGTCTC 1 cut(s) 1312
BsoBI CYCGRG 1 cut(s) 170
Bsp1286I GDGCHC 2 cut(s) 395, 1364
Bsp1407I TGTACA 2 cut(s) 355, 634
Bsp143I GATC 4 cut(s) 265, 807, 849, 1108
Bsp19I CCATGG 1 cut(s) 12
BspACI CCGC 5 cut(s) 32, 51, 68, 802, 1046
BspANI GGCC 1 cut(s) 309
BspCNI CTCAG 5 cut(s) 409, 489, 1015, 1205, 1267
BspFNI CGCG 2 cut(s) 32, 1046
BspLI GGNNCC 4 cut(s) 185, 725, 840, 948
BspMAI CTGCAG 1 cut(s) 964
BspPI GGATC 2 cut(s) 260, 1103
BspTNI GGTCTC 1 cut(s) 1312
BsrDI GCAATG 1 cut(s) 1165
BsrGI TGTACA 2 cut(s) 355, 634
BsrI ACTGG 4 cut(s) 673, 1030, 1112, 1204
BssECI CCNNGG 5 cut(s) 12, 180, 428, 695, 727
BssMI GATC 4 cut(s) 265, 807, 849, 1108
BssNI GRCGYC 2 cut(s) 35, 117
BssT1I CCWWGG 3 cut(s) 12, 695, 727
Bst2UI CCWGG 5 cut(s) 130, 181, 429, 593, 1371
Bst4CI ACNGT 3 cut(s) 331, 473, 1417
Bst6I CTCTTC 2 cut(s) 435, 1077
BstACI GRCGYC 2 cut(s) 35, 117
BstAPI GCANNNNNTGC 1 cut(s) 550
BstAUI TGTACA 2 cut(s) 355, 634
BstC8I GCNNGC 3 cut(s) 338, 513, 1360
BstDEI CTNAG 7 cut(s) 302, 396, 497, 831, 1023, 1192, 1275
BstDSI CCRYGG 1 cut(s) 12
BstEII GGTNACC 1 cut(s) 931
BstENI CCTNNNNNAGG 2 cut(s) 596, 941
BstF5I GGATG 3 cut(s) 106, 1243, 1457
BstFNI CGCG 2 cut(s) 32, 1046
BstKTI GATC 4 cut(s) 268, 810, 852, 1111
BstMAI GTCTC 4 cut(s) 130, 682, 1190, 1312
BstMBI GATC 4 cut(s) 265, 807, 849, 1108
BstMWI GCNNNNNNNGC 8 cut(s) 147, 502, 508, 550, 571, 637, 791, 1320
BstNI CCWGG 5 cut(s) 130, 181, 429, 593, 1371
BstNSI RCATGY 3 cut(s) 411, 1093, 1225
BstPAI GACNNNNGTC 1 cut(s) 1461
BstPI GGTNACC 1 cut(s) 931
BstSCI CCNGG 6 cut(s) 128, 179, 427, 591, 613, 1369
BstSFI CTRYAG 3 cut(s) 960, 1011, 1251
BstUI CGCG 2 cut(s) 32, 1046
BstV1I GCAGC 7 cut(s) 514, 583, 586, 744, 786, 797, 1507
BstV2I GAAGAC 3 cut(s) 33, 332, 1026
BstX2I RGATCY 2 cut(s) 807, 1108
BstXI CCANNNNNNTGG 1 cut(s) 276
BstYI RGATCY 2 cut(s) 807, 1108
BsuI GTATCC 1 cut(s) 1205
BsuRI GGCC 1 cut(s) 309
BtgI CCRYGG 1 cut(s) 12
BtsCI GGATG 3 cut(s) 106, 1243, 1457
BtsI GCAGTG 1 cut(s) 1364
BtsIMutI CAGTG 3 cut(s) 666, 1364, 1482
Cac8I GCNNGC 3 cut(s) 338, 513, 1360
CaiI CAGNNNCTG 1 cut(s) 1199
Cfr13I GGNCC 2 cut(s) 184, 1027
CseI GACGC 3 cut(s) 24, 38, 125
Csp6I GTAC 3 cut(s) 356, 635, 763
CviAII CATG 8 cut(s) 13, 94, 353, 408, 796, 1090, 1216, 1222
CviQI GTAC 3 cut(s) 356, 635, 763
DdeI CTNAG 7 cut(s) 302, 396, 497, 831, 1023, 1192, 1275
DpnI GATC 4 cut(s) 267, 809, 851, 1110
DpnII GATC 4 cut(s) 265, 807, 849, 1108
DrdI GACNNNNNNGTC 1 cut(s) 122
DriI GACNNNNNGTC 1 cut(s) 34
DseDI GACNNNNNNGTC 1 cut(s) 122
Eam1104I CTCTTC 2 cut(s) 435, 1077
Eam1105I GACNNNNNGTC 1 cut(s) 34
EarI CTCTTC 2 cut(s) 435, 1077
EciI GGCGGA 2 cut(s) 40, 57
Ecl136II GAGCTC 1 cut(s) 393
Eco130I CCWWGG 3 cut(s) 12, 695, 727
Eco24I GRGCYC 2 cut(s) 395, 1364
Eco31I GGTCTC 1 cut(s) 1312
Eco32I GATATC 1 cut(s) 1333
Eco47I GGWCC 2 cut(s) 184, 1027
Eco53kI GAGCTC 1 cut(s) 393
Eco88I CYCGRG 1 cut(s) 170
Eco91I GGTNACC 1 cut(s) 931
EcoICRI GAGCTC 1 cut(s) 393
EcoNI CCTNNNNNAGG 2 cut(s) 596, 941
EcoO109I RGGNCCY 1 cut(s) 184
EcoO65I GGTNACC 1 cut(s) 931
EcoRII CCWGG 5 cut(s) 128, 179, 427, 591, 1369
EcoRV GATATC 1 cut(s) 1333
EcoT14I CCWWGG 3 cut(s) 12, 695, 727
EcoT22I ATGCAT 1 cut(s) 609
EcoT38I GRGCYC 2 cut(s) 395, 1364
ErhI CCWWGG 3 cut(s) 12, 695, 727
Esp3I CGTCTC 1 cut(s) 130
FaeI CATG 8 cut(s) 16, 97, 356, 411, 799, 1093, 1219, 1225
FaqI GGGAC 4 cut(s) 152, 197, 597, 1469
FatI CATG 8 cut(s) 12, 93, 352, 407, 795, 1089, 1215, 1221
FauNDI CATATG 1 cut(s) 1470
FbaI TGATCA 1 cut(s) 849
Fnu4HI GCNGC 9 cut(s) 33, 503, 572, 575, 733, 786, 800, 803, 1496
FokI GGATG 3 cut(s) 113, 1250, 1464
FriOI GRGCYC 2 cut(s) 395, 1364
Fsp4HI GCNGC 9 cut(s) 33, 503, 572, 575, 733, 786, 800, 803, 1496
FspBI CTAG 6 cut(s) 222, 434, 903, 915, 987, 1311
GluI GCNGC 9 cut(s) 33, 503, 572, 575, 733, 786, 800, 803, 1496
GsaI CCCAGC 1 cut(s) 631
GsuI CTGGAG 2 cut(s) 151, 163
HaeIII GGCC 1 cut(s) 309
HapII CCGG 1 cut(s) 615
HgaI GACGC 3 cut(s) 24, 38, 125
Hin1I GRCGYC 2 cut(s) 35, 117
Hin1II CATG 8 cut(s) 16, 97, 356, 411, 799, 1093, 1219, 1225
HincII GTYRAC 2 cut(s) 1227, 1466
HindII GTYRAC 2 cut(s) 1227, 1466
HinfI GANTC 7 cut(s) 21, 361, 690, 983, 1420, 1462, 1474
HpaII CCGG 1 cut(s) 615
HphI GGTGA 3 cut(s) 769, 943, 1385
Hpy166II GTNNAC 2 cut(s) 1227, 1466
Hpy188I TCNGA 6 cut(s) 78, 921, 943, 1024, 1195, 1402
Hpy188III TCNNGA 8 cut(s) 160, 269, 743, 811, 857, 987, 1274, 1424
Hpy8I GTNNAC 2 cut(s) 1227, 1466
Hpy99I CGWCG 4 cut(s) 40, 119, 125, 158
HpyAV CCTTC 5 cut(s) 287, 780, 894, 1325, 1435
HpyCH4III ACNGT 3 cut(s) 331, 473, 1417
HpyCH4IV ACGT 1 cut(s) 547
HpyF10VI GCNNNNNNNGC 8 cut(s) 147, 502, 508, 550, 571, 637, 791, 1320
HpyF3I CTNAG 7 cut(s) 302, 396, 497, 831, 1023, 1192, 1275
HpySE526I ACGT 1 cut(s) 547
Hsp92I GRCGYC 2 cut(s) 35, 117
Hsp92II CATG 8 cut(s) 16, 97, 356, 411, 799, 1093, 1219, 1225
Ksp22I TGATCA 1 cut(s) 849
Kzo9I GATC 4 cut(s) 265, 807, 849, 1108
LmnI GCTCC 3 cut(s) 132, 562, 1512
Lsp1109I GCAGC 7 cut(s) 514, 583, 586, 744, 786, 797, 1507
LweI GCATC 5 cut(s) 649, 850, 1273, 1279, 1514
MaeI CTAG 6 cut(s) 222, 434, 903, 915, 987, 1311
MaeII ACGT 1 cut(s) 547
MaeIII GTNAC 3 cut(s) 931, 1373, 1411
MalI GATC 4 cut(s) 267, 809, 851, 1110
MboI GATC 4 cut(s) 265, 807, 849, 1108
MboII GAAGA 7 cut(s) 38, 337, 452, 826, 1026, 1094, 1327
MflI RGATCY 2 cut(s) 807, 1108
MhlI GDGCHC 2 cut(s) 395, 1364
MluCI AATT 8 cut(s) 281, 711, 870, 954, 1096, 1346, 1386, 1518
MlyI GAGTC 4 cut(s) 30, 684, 992, 1471
MmeI TCCRAC 2 cut(s) 899, 1077
Mph1103I ATGCAT 1 cut(s) 609
MroXI GAANNNNTTC 2 cut(s) 297, 1347
MseI TTAA 4 cut(s) 456, 1062, 1095, 1326
MslI CAYNNNNRTG 2 cut(s) 645, 1220
MspI CCGG 1 cut(s) 615
MspR9I CCNGG 6 cut(s) 130, 181, 429, 593, 615, 1371
Mva1269I GAATGC 2 cut(s) 1140, 1210
MvaI CCWGG 5 cut(s) 130, 181, 429, 593, 1371
MvnI CGCG 2 cut(s) 32, 1046
MwoI GCNNNNNNNGC 8 cut(s) 147, 502, 508, 550, 571, 637, 791, 1320
NciI CCSGG 1 cut(s) 615
NcoI CCATGG 1 cut(s) 12
NdeI CATATG 1 cut(s) 1470
NdeII GATC 4 cut(s) 265, 807, 849, 1108
NlaIII CATG 8 cut(s) 16, 97, 356, 411, 799, 1093, 1219, 1225
NlaIV GGNNCC 4 cut(s) 185, 725, 840, 948
NmuCI GTSAC 3 cut(s) 931, 1373, 1411
NsiI ATGCAT 1 cut(s) 609
NspI RCATGY 3 cut(s) 411, 1093, 1225
PctI GAATGC 2 cut(s) 1140, 1210
PdmI GAANNNNTTC 2 cut(s) 297, 1347
PfeI GAWTC 3 cut(s) 361, 1420, 1474
PflFI GACNNNGTC 1 cut(s) 119
PflMI CCANNNNNTGG 1 cut(s) 1370
PfoI TCCNGGA 2 cut(s) 128, 613
PkrI GCNGC 9 cut(s) 34, 504, 573, 576, 734, 787, 801, 804, 1497
PleI GAGTC 4 cut(s) 29, 684, 991, 1470
PpsI GAGTC 4 cut(s) 29, 684, 991, 1470
PpuMI RGGWCCY 1 cut(s) 184
PshAI GACNNNNGTC 1 cut(s) 1461
PshBI ATTAAT 1 cut(s) 1326
Psp124BI GAGCTC 1 cut(s) 395
Psp5II RGGWCCY 1 cut(s) 184
Psp6I CCWGG 5 cut(s) 128, 179, 427, 591, 1369
PspEI GGTNACC 1 cut(s) 931
PspFI CCCAGC 1 cut(s) 627
PspGI CCWGG 5 cut(s) 128, 179, 427, 591, 1369
PspN4I GGNNCC 4 cut(s) 185, 725, 840, 948
PspPI GGNCC 2 cut(s) 184, 1027
PspPPI RGGWCCY 1 cut(s) 184
PsrI GAACNNNNNNTAC 2 cut(s) 71, 103
PstI CTGCAG 1 cut(s) 964
PstNI CAGNNNCTG 1 cut(s) 1199
PsuI RGATCY 2 cut(s) 807, 1108
PsyI GACNNNGTC 1 cut(s) 119
RsaI GTAC 3 cut(s) 357, 636, 764
RsaNI GTAC 3 cut(s) 356, 635, 763
RseI CAYNNNNRTG 2 cut(s) 645, 1220
SacI GAGCTC 1 cut(s) 395
SaqAI TTAA 4 cut(s) 456, 1062, 1095, 1326
SatI GCNGC 9 cut(s) 33, 503, 572, 575, 733, 786, 800, 803, 1496
Sau3AI GATC 4 cut(s) 265, 807, 849, 1108
Sau96I GGNCC 2 cut(s) 184, 1027
SchI GAGTC 4 cut(s) 30, 684, 992, 1471
ScrFI CCNGG 6 cut(s) 130, 181, 429, 593, 615, 1371
SduI GDGCHC 2 cut(s) 395, 1364
SfaNI GCATC 5 cut(s) 649, 850, 1273, 1279, 1514
SfcI CTRYAG 3 cut(s) 960, 1011, 1251
SinI GGWCC 2 cut(s) 184, 1027
SmiMI CAYNNNNRTG 2 cut(s) 645, 1220
SmlI CTYRAG 2 cut(s) 381, 1071
SmoI CTYRAG 2 cut(s) 381, 1071
Sse9I AATT 8 cut(s) 281, 711, 870, 954, 1096, 1346, 1386, 1518
SsiI CCGC 5 cut(s) 32, 51, 68, 802, 1046
SspMI CTAG 6 cut(s) 222, 434, 903, 915, 987, 1311
SstI GAGCTC 1 cut(s) 395
StyD4I CCNGG 6 cut(s) 128, 179, 427, 591, 613, 1369
StyI CCWWGG 3 cut(s) 12, 695, 727
TaaI ACNGT 3 cut(s) 331, 473, 1417
TaiI ACGT 1 cut(s) 550
TaqI TCGA 3 cut(s) 24, 447, 1132
TaqII GACCGA 1 cut(s) 288
TasI AATT 8 cut(s) 281, 711, 870, 954, 1096, 1346, 1386, 1518
TatI WGTACW 2 cut(s) 355, 634
TauI GCSGC 2 cut(s) 35, 805
TfiI GAWTC 3 cut(s) 361, 1420, 1474
Tru1I TTAA 4 cut(s) 456, 1062, 1095, 1326
Tru9I TTAA 4 cut(s) 456, 1062, 1095, 1326
TscAI CASTG 3 cut(s) 673, 1371, 1489
TseFI GTSAC 3 cut(s) 931, 1373, 1411
TseI GCWGC 7 cut(s) 502, 571, 574, 732, 785, 799, 1495
Tsp45I GTSAC 3 cut(s) 931, 1373, 1411
TspDTI ATGAA 8 cut(s) 306, 663, 723, 791, 795, 833, 1174, 1487
TspRI CASTG 3 cut(s) 673, 1371, 1489
Tth111I GACNNNGTC 1 cut(s) 119
Van91I CCANNNNNTGG 1 cut(s) 1370
VpaK11BI GGWCC 2 cut(s) 184, 1027
VspI ATTAAT 1 cut(s) 1326
XagI CCTNNNNNAGG 2 cut(s) 596, 941
XapI RAATTY 2 cut(s) 281, 711
XbaI TCTAGA 1 cut(s) 986
XceI RCATGY 3 cut(s) 411, 1093, 1225
XmnI GAANNNNTTC 2 cut(s) 297, 1347
XspI CTAG 6 cut(s) 222, 434, 903, 915, 987, 1311
Zsp2I ATGCAT 1 cut(s) 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.