pycom05g19750

Heat stress transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0008306
Physical Location & Seq
Forward (+)
22629050 .. 22631768
2719 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g19750.1

Sequence Viewer

Length: 1128 bp
ATGCAAACAACCATGGAGGGAATCGAAGACGCCGCGTCGGTCATCAACTCCGCCAACACTCCTCCTCCGTTTCTCAATAAAACCTACGATATGGTGGATGACCACTCCACCGACGCCGTCGTCTCCTGGAGCAACGGCAACAACAGCTTCGTCGTCTGGAACGTCCCCGAGTTCTCGAGGGACGTCCTGCCCAAGTATTTCAAGCACAACAACTTCTCTAGCTTCGTCAGGCAGTTGAATACTTATGGTTTTAGAAAGGTTGATCCAGACCGATGGGAATTTGCAAATGAAGGGTTTCTTAGAGGCCAGAAACACCTTTTGAAGACTGTAAGCAGGCGAAAACCAGCTCATGTACAGAGTCATCAACAACCACCACCTCAAGTGCAGAGCTCTCAGGTTGGGGCATGTGTAGAGGTTGTAAATCCTGGGCTGGAGGAAGAGGTTGAAAGACTTAAAAGAGATAAGAACACTCTTATGCAGGAACTTGTTAGGCTGAGGCAGCAACAGCAAGCAACAGATAATCAGTTGCATAATGTTGGGCAACGTATGCAGGGAATGGAGCAGCGGCAGCAGCAAATGATGTCATTTCTTGCAAAGGCTATGCATAGTCCCGGATTTTTATCACAGCTTGTACAGCATCAGAATGAAAACAATAGGCGCATCACTGGAAGCAATAAGAAAAGGAGACTCCCAAGGCAAGAAGATGAAATTTTAGTTGGGAACCTTGGCAGCAAAGTTCTTGATGGACAAATGGTGAAGTACCAGCCTTCAATGAATGAAGCAGCAAAAGCAATGCTGCGGCAGATCTTGAAGATGAATACATCTCCAAAGCTGGAACCATCATTGATCAATCCTGATGCTTTCCTAATTGATAATGTTCCTTCCAATGCAACAGATAATAGGAACACCTCTAGTCGGAACTTAGGGGTGACCCTTTCAGAGGTTCCACCAATTTCTGCAGAGTGTTATATGCCTGAAGAGTCTAGATTTCCTGTCAGTTGTCATTCTACAGCCATTTCTGATCGATCCAGTCTTCCCCTTATGCGGTTGCCAACTCTGTTAAAGAAACTCAAGTTTTGGAAGAGAACATGCATAATTTTCAACAAGATACAGCTATCCCTGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSF_DNA-bind

Protein Analysis

376

Amino Acids

42.79

Weight (kDa)

9.45

Isoelectric Point (pI)

61.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 24 - 113 7.7e-30 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 119
AatII GACGTC 1 cut(s) 186
AccII CGCG 1 cut(s) 35
AciI CCGC 5 cut(s) 33, 51, 565, 799, 1045
AclWI GGATC 2 cut(s) 257, 1020
AcsI RAATTY 2 cut(s) 278, 708
AcuI CTGAAG 1 cut(s) 996
AcyI GRCGYC 3 cut(s) 30, 114, 183
AfaI GTAC 3 cut(s) 354, 633, 761
AfiI CCNNNNNNNGG 3 cut(s) 915, 940, 1044
AgsI TTSAA 7 cut(s) 202, 238, 322, 446, 771, 811, 1102
AhdI GACNNNNNGTC 1 cut(s) 34
AjnI CCWGG 2 cut(s) 125, 424
AjuI GAANNNNNNNTTGG 2 cut(s) 699, 731
AluBI AGCT 7 cut(s) 147, 222, 347, 390, 628, 832, 1114
AluI AGCT 7 cut(s) 147, 222, 347, 390, 628, 832, 1114
Alw21I GWGCWC 1 cut(s) 392
Alw26I GTCTC 2 cut(s) 127, 679
AlwI GGATC 2 cut(s) 257, 1020
Ama87I CYCGRG 2 cut(s) 167, 175
AoxI GGCC 1 cut(s) 304
ApeKI GCWGC 7 cut(s) 499, 562, 568, 571, 729, 782, 796
ApoI RAATTY 2 cut(s) 278, 708
Asp700I GAANNNNTTC 1 cut(s) 294
AspLEI GCGC 1 cut(s) 660
AsuC2I CCSGG 1 cut(s) 612
AsuHPI GGTGA 2 cut(s) 766, 940
AvaI CYCGRG 2 cut(s) 167, 175
BanII GRGCYC 1 cut(s) 392
BbsI GAAGAC 3 cut(s) 33, 329, 1025
Bbv12I GWGCWC 1 cut(s) 392
BbvCI CCTCAGC 1 cut(s) 494
BbvI GCAGC 7 cut(s) 511, 574, 580, 583, 741, 783, 794
BccI CCATC 3 cut(s) 267, 737, 847
BceAI ACGGC 2 cut(s) 101, 151
BciT130I CCWGG 2 cut(s) 127, 426
BclI TGATCA 1 cut(s) 846
BcnI CCSGG 1 cut(s) 612
BcoDI GTCTC 2 cut(s) 127, 679
BfaI CTAG 3 cut(s) 219, 912, 984
BfmI CTRYAG 2 cut(s) 957, 1008
BglII AGATCT 1 cut(s) 804
Bme1390I CCNGG 3 cut(s) 127, 426, 612
BmeRI GACNNNNNGTC 1 cut(s) 34
BmeT110I CYCGRG 2 cut(s) 167, 175
BmiI GGNNCC 3 cut(s) 722, 837, 945
BmrFI CCNGG 3 cut(s) 127, 426, 612
BmsI GCATC 3 cut(s) 646, 669, 847
BpiI GAAGAC 3 cut(s) 33, 329, 1025
BpmI CTGGAG 2 cut(s) 148, 452
Bpu10I CCTNAGC 1 cut(s) 494
BpuEI CTTGAG 2 cut(s) 363, 1055
BpuMI CCSGG 1 cut(s) 612
Bsa29I ATCGAT 1 cut(s) 1024
BsaBI GATNNNNATC 1 cut(s) 619
BsaHI GRCGYC 3 cut(s) 30, 114, 183
BsaJI CCNNGG 4 cut(s) 12, 425, 692, 724
BsaXI ACNNNNNCTCC 2 cut(s) 49, 79
Bsc4I CCNNNNNNNGG 3 cut(s) 915, 940, 1044
Bse1I ACTGG 2 cut(s) 670, 1029
Bse3DI GCAATG 1 cut(s) 798
Bse8I GATNNNNATC 1 cut(s) 619
BseBI CCWGG 2 cut(s) 127, 426
BseCI ATCGAT 1 cut(s) 1024
BseDI CCNNGG 4 cut(s) 12, 425, 692, 724
BseGI GGATG 1 cut(s) 103
BseJI GATNNNNATC 1 cut(s) 619
BseLI CCNNNNNNNGG 3 cut(s) 915, 940, 1044
BseMI GCAATG 1 cut(s) 798
BseMII CTCAG 2 cut(s) 407, 485
BseNI ACTGG 2 cut(s) 670, 1029
BseRI GAGGAG 2 cut(s) 51, 54
BseXI GCAGC 7 cut(s) 511, 574, 580, 583, 741, 783, 794
BsgI GTGCAG 1 cut(s) 404
Bsh1236I CGCG 1 cut(s) 35
BshFI GGCC 1 cut(s) 306
BshVI ATCGAT 1 cut(s) 1024
BsiHKAI GWGCWC 1 cut(s) 392
BsiHKCI CYCGRG 2 cut(s) 167, 175
BsiSI CCGG 1 cut(s) 612
BslFI GGGAC 3 cut(s) 149, 194, 594
BslI CCNNNNNNNGG 3 cut(s) 915, 940, 1044
BsmAI GTCTC 2 cut(s) 127, 679
BsmBI CGTCTC 1 cut(s) 127
BsmFI GGGAC 3 cut(s) 149, 194, 594
BsnI GGCC 1 cut(s) 306
BsoBI CYCGRG 2 cut(s) 167, 175
Bsp1286I GDGCHC 1 cut(s) 392
Bsp1407I TGTACA 2 cut(s) 352, 631
Bsp143I GATC 5 cut(s) 262, 804, 846, 1021, 1025
Bsp19I CCATGG 1 cut(s) 12
BspACI CCGC 5 cut(s) 33, 51, 565, 799, 1045
BspANI GGCC 1 cut(s) 306
BspCNI CTCAG 2 cut(s) 406, 486
BspDI ATCGAT 1 cut(s) 1024
BspFNI CGCG 1 cut(s) 35
BspLI GGNNCC 3 cut(s) 722, 837, 945
BspMAI CTGCAG 1 cut(s) 961
BspPI GGATC 2 cut(s) 257, 1020
BsrDI GCAATG 1 cut(s) 798
BsrGI TGTACA 2 cut(s) 352, 631
BsrI ACTGG 2 cut(s) 670, 1029
BssECI CCNNGG 4 cut(s) 12, 425, 692, 724
BssMI GATC 5 cut(s) 262, 804, 846, 1021, 1025
BssNI GRCGYC 3 cut(s) 30, 114, 183
BssT1I CCWWGG 3 cut(s) 12, 692, 724
Bst2UI CCWGG 2 cut(s) 127, 426
Bst4CI ACNGT 1 cut(s) 328
Bst6I CTCTTC 3 cut(s) 432, 972, 1076
BstACI GRCGYC 3 cut(s) 30, 114, 183
BstAPI GCANNNNNTGC 1 cut(s) 547
BstAUI TGTACA 2 cut(s) 352, 631
BstC8I GCNNGC 2 cut(s) 335, 510
BstDEI CTNAG 4 cut(s) 299, 393, 494, 922
BstDSI CCRYGG 1 cut(s) 12
BstEII GGTNACC 1 cut(s) 928
BstENI CCTNNNNNAGG 1 cut(s) 938
BstF5I GGATG 1 cut(s) 103
BstFNI CGCG 1 cut(s) 35
BstHHI GCGC 1 cut(s) 660
BstKTI GATC 5 cut(s) 265, 807, 849, 1024, 1028
BstMAI GTCTC 2 cut(s) 127, 679
BstMBI GATC 5 cut(s) 262, 804, 846, 1021, 1025
BstMWI GCNNNNNNNGC 8 cut(s) 144, 499, 505, 547, 568, 571, 634, 788
BstNI CCWGG 2 cut(s) 127, 426
BstNSI RCATGY 2 cut(s) 408, 1092
BstPI GGTNACC 1 cut(s) 928
BstSCI CCNGG 3 cut(s) 125, 424, 610
BstSFI CTRYAG 2 cut(s) 957, 1008
BstUI CGCG 1 cut(s) 35
BstV1I GCAGC 7 cut(s) 511, 574, 580, 583, 741, 783, 794
BstV2I GAAGAC 3 cut(s) 33, 329, 1025
BstX2I RGATCY 1 cut(s) 804
BstXI CCANNNNNNTGG 1 cut(s) 273
BstYI RGATCY 1 cut(s) 804
Bsu15I ATCGAT 1 cut(s) 1024
BsuRI GGCC 1 cut(s) 306
BsuTUI ATCGAT 1 cut(s) 1024
BtgI CCRYGG 1 cut(s) 12
BtsCI GGATG 1 cut(s) 103
BtsIMutI CAGTG 1 cut(s) 663
Cac8I GCNNGC 2 cut(s) 335, 510
CfoI GCGC 1 cut(s) 660
ClaI ATCGAT 1 cut(s) 1024
CseI GACGC 3 cut(s) 24, 38, 122
Csp6I GTAC 3 cut(s) 353, 632, 760
CviAII CATG 4 cut(s) 13, 350, 405, 1089
CviQI GTAC 3 cut(s) 353, 632, 760
DdeI CTNAG 4 cut(s) 299, 393, 494, 922
DpnI GATC 5 cut(s) 264, 806, 848, 1023, 1027
DpnII GATC 5 cut(s) 262, 804, 846, 1021, 1025
DrdI GACNNNNNNGTC 1 cut(s) 119
DriI GACNNNNNGTC 1 cut(s) 34
DseDI GACNNNNNNGTC 1 cut(s) 119
Eam1104I CTCTTC 3 cut(s) 432, 972, 1076
Eam1105I GACNNNNNGTC 1 cut(s) 34
EarI CTCTTC 3 cut(s) 432, 972, 1076
EciI GGCGGA 1 cut(s) 40
Ecl136II GAGCTC 1 cut(s) 390
Eco130I CCWWGG 3 cut(s) 12, 692, 724
Eco24I GRGCYC 1 cut(s) 392
Eco53kI GAGCTC 1 cut(s) 390
Eco57I CTGAAG 1 cut(s) 996
Eco88I CYCGRG 2 cut(s) 167, 175
Eco91I GGTNACC 1 cut(s) 928
EcoICRI GAGCTC 1 cut(s) 390
EcoNI CCTNNNNNAGG 1 cut(s) 938
EcoO65I GGTNACC 1 cut(s) 928
EcoRII CCWGG 2 cut(s) 125, 424
EcoT14I CCWWGG 3 cut(s) 12, 692, 724
EcoT22I ATGCAT 2 cut(s) 606, 1094
EcoT38I GRGCYC 1 cut(s) 392
ErhI CCWWGG 3 cut(s) 12, 692, 724
Esp3I CGTCTC 1 cut(s) 127
FaeI CATG 4 cut(s) 16, 353, 408, 1092
FalI AAGNNNNNCTT 2 cut(s) 282, 314
FaqI GGGAC 3 cut(s) 149, 194, 594
FatI CATG 4 cut(s) 12, 349, 404, 1088
FbaI TGATCA 1 cut(s) 846
FokI GGATG 1 cut(s) 110
FriOI GRGCYC 1 cut(s) 392
FspBI CTAG 3 cut(s) 219, 912, 984
GlaI GCGC 1 cut(s) 659
GsuI CTGGAG 2 cut(s) 148, 452
HaeIII GGCC 1 cut(s) 306
HapII CCGG 1 cut(s) 612
HgaI GACGC 3 cut(s) 24, 38, 122
HhaI GCGC 1 cut(s) 660
Hin1I GRCGYC 3 cut(s) 30, 114, 183
Hin1II CATG 4 cut(s) 16, 353, 408, 1092
Hin6I GCGC 1 cut(s) 658
HinP1I GCGC 1 cut(s) 658
HinfI GANTC 4 cut(s) 21, 358, 687, 980
HpaII CCGG 1 cut(s) 612
HphI GGTGA 2 cut(s) 766, 940
Hpy188I TCNGA 4 cut(s) 642, 918, 940, 1021
Hpy188III TCNNGA 7 cut(s) 157, 175, 266, 740, 808, 854, 984
Hpy99I CGWCG 4 cut(s) 40, 116, 122, 155
HpyAV CCTTC 3 cut(s) 284, 777, 891
HpyCH4III ACNGT 1 cut(s) 328
HpyCH4IV ACGT 3 cut(s) 162, 183, 544
HpyF10VI GCNNNNNNNGC 8 cut(s) 144, 499, 505, 547, 568, 571, 634, 788
HpyF3I CTNAG 4 cut(s) 299, 393, 494, 922
HpySE526I ACGT 3 cut(s) 162, 183, 544
Hsp92I GRCGYC 3 cut(s) 30, 114, 183
Hsp92II CATG 4 cut(s) 16, 353, 408, 1092
HspAI GCGC 1 cut(s) 658
Ksp22I TGATCA 1 cut(s) 846
Kzo9I GATC 5 cut(s) 262, 804, 846, 1021, 1025
LmnI GCTCC 2 cut(s) 129, 559
Lsp1109I GCAGC 7 cut(s) 511, 574, 580, 583, 741, 783, 794
LweI GCATC 3 cut(s) 646, 669, 847
MaeI CTAG 3 cut(s) 219, 912, 984
MaeII ACGT 3 cut(s) 162, 183, 544
MaeIII GTNAC 1 cut(s) 928
MalI GATC 5 cut(s) 264, 806, 848, 1023, 1027
MboI GATC 5 cut(s) 262, 804, 846, 1021, 1025
MboII GAAGA 8 cut(s) 38, 334, 449, 713, 823, 989, 1025, 1093
MflI RGATCY 1 cut(s) 804
MhlI GDGCHC 1 cut(s) 392
MluCI AATT 5 cut(s) 278, 708, 867, 951, 1095
MlyI GAGTC 3 cut(s) 367, 681, 989
MmeI TCCRAC 1 cut(s) 896
Mph1103I ATGCAT 2 cut(s) 606, 1094
MroXI GAANNNNTTC 1 cut(s) 294
MseI TTAA 2 cut(s) 453, 1061
MslI CAYNNNNRTG 2 cut(s) 473, 642
MspA1I CMGCKG 1 cut(s) 565
MspI CCGG 1 cut(s) 612
MspR9I CCNGG 3 cut(s) 127, 426, 612
MvaI CCWGG 2 cut(s) 127, 426
MvnI CGCG 1 cut(s) 35
MwoI GCNNNNNNNGC 8 cut(s) 144, 499, 505, 547, 568, 571, 634, 788
NciI CCSGG 1 cut(s) 612
NcoI CCATGG 1 cut(s) 12
NdeII GATC 5 cut(s) 262, 804, 846, 1021, 1025
NlaIII CATG 4 cut(s) 16, 353, 408, 1092
NlaIV GGNNCC 3 cut(s) 722, 837, 945
NmuCI GTSAC 1 cut(s) 928
NsiI ATGCAT 2 cut(s) 606, 1094
NspI RCATGY 2 cut(s) 408, 1092
PaeR7I CTCGAG 1 cut(s) 175
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PdmI GAANNNNTTC 1 cut(s) 294
PfeI GAWTC 1 cut(s) 21
PflFI GACNNNGTC 1 cut(s) 116
PfoI TCCNGGA 2 cut(s) 125, 610
PleI GAGTC 3 cut(s) 366, 681, 988
PpsI GAGTC 3 cut(s) 366, 681, 988
Psp124BI GAGCTC 1 cut(s) 392
Psp6I CCWGG 2 cut(s) 125, 424
PspEI GGTNACC 1 cut(s) 928
PspGI CCWGG 2 cut(s) 125, 424
PspN4I GGNNCC 3 cut(s) 722, 837, 945
PstI CTGCAG 1 cut(s) 961
PsuI RGATCY 1 cut(s) 804
PsyI GACNNNGTC 1 cut(s) 116
RsaI GTAC 3 cut(s) 354, 633, 761
RsaNI GTAC 3 cut(s) 353, 632, 760
RseI CAYNNNNRTG 2 cut(s) 473, 642
SacI GAGCTC 1 cut(s) 392
SaqAI TTAA 2 cut(s) 453, 1061
Sau3AI GATC 5 cut(s) 262, 804, 846, 1021, 1025
SchI GAGTC 3 cut(s) 367, 681, 989
ScrFI CCNGG 3 cut(s) 127, 426, 612
SduI GDGCHC 1 cut(s) 392
SfaNI GCATC 3 cut(s) 646, 669, 847
SfcI CTRYAG 2 cut(s) 957, 1008
Sfr274I CTCGAG 1 cut(s) 175
SlaI CTCGAG 1 cut(s) 175
SmiMI CAYNNNNRTG 2 cut(s) 473, 642
SmlI CTYRAG 3 cut(s) 175, 378, 1070
SmoI CTYRAG 3 cut(s) 175, 378, 1070
Sse9I AATT 5 cut(s) 278, 708, 867, 951, 1095
SsiI CCGC 5 cut(s) 33, 51, 565, 799, 1045
SspMI CTAG 3 cut(s) 219, 912, 984
SstI GAGCTC 1 cut(s) 392
StyD4I CCNGG 3 cut(s) 125, 424, 610
StyI CCWWGG 3 cut(s) 12, 692, 724
TaaI ACNGT 1 cut(s) 328
TaiI ACGT 3 cut(s) 165, 186, 547
TaqI TCGA 3 cut(s) 24, 176, 1024
TaqII GACCGA 2 cut(s) 28, 285
TasI AATT 5 cut(s) 278, 708, 867, 951, 1095
TatI WGTACW 2 cut(s) 352, 631
TauI GCSGC 3 cut(s) 35, 568, 802
TfiI GAWTC 1 cut(s) 21
Tru1I TTAA 2 cut(s) 453, 1061
Tru9I TTAA 2 cut(s) 453, 1061
TscAI CASTG 1 cut(s) 670
TseFI GTSAC 1 cut(s) 928
TseI GCWGC 7 cut(s) 499, 562, 568, 571, 729, 782, 796
Tsp45I GTSAC 1 cut(s) 928
TspDTI ATGAA 6 cut(s) 303, 660, 720, 788, 792, 830
TspGWI ACGGA 1 cut(s) 57
TspRI CASTG 1 cut(s) 670
Tth111I GACNNNGTC 1 cut(s) 116
XagI CCTNNNNNAGG 1 cut(s) 938
XapI RAATTY 2 cut(s) 278, 708
XbaI TCTAGA 1 cut(s) 983
XceI RCATGY 2 cut(s) 408, 1092
XhoI CTCGAG 1 cut(s) 175
XmnI GAANNNNTTC 1 cut(s) 294
XspI CTAG 3 cut(s) 219, 912, 984
ZraI GACGTC 1 cut(s) 184
Zsp2I ATGCAT 2 cut(s) 606, 1094
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.