RchiOBHm_Chr5g0027071

Heat stress transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0008306
Physical Location & Seq
Reverse (-)
21026544 .. 21030345
3802 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30662

Sequence Viewer

Length: 1479 bp
ATGGAAGACGCGGCGACGATGATCAACTCGGCGACGAGCATCCCGCCGTTTCTGAACAAGACCTACGACATGGTCGACGACCCCTCCACCGACTACTTGGTCTCATGGACCGACGCCAACAACAGTTTCGTCGTTTGGAACGACGCCCAGTTCGCCCGGGACCTGTTGCCCAAGTTCTTCAAGCACAACAACTTCTCCAGCTTCGTCCGCCAGCTCAATACTTATGGTTTTAGAAAAGTTCATCCAGACCGATGGGAATTCGCAAATGAAGGGTTTCTAAGAGGTCAGAAACATCTTTTGAAGACTGTCAATAGGCGAAAGCCAGTTAATGTACAGAGTCATCAACAATCAGCACCAGTACCAGCACCTAAAGTAAAGAGCTCCCAAGTTGCTGCATGCGTAGAGGTGGGAAAACTTGGACTGGAGGAGGAGGTTGAAAGGCTTAAAAGGGACAAGAACACTCTTATGCAGGAACTTATTAGTTTGAGGCAGGAGCAGCAAGCAACAGATAACCAATTACAGAATGTTGGTCAACGTGTGCAGGGAATGGAACAGCGGCAGAAGCAAATGATGTCATTTCTTGCAAAGGCTATGCATAGTCCTGGCTTTTTATCCCAGCTTGTACAGCATCAGAATGAGAGCAACAGGCGCATCACTGGAAGCAACAAGAAAAGGAGACTTCCGAGGCAAGAAGACGAAATTTTGGTGGGGAAGCTTAGCAGTAAAACTCTGGATGGACAAATTGTGAAATACCAGCCTTCAATGAATGAAGCAGCAAAAGCAATGCTACGGCAGATTATGAAGATGAATATATCTCCTAGGCGGGAACCGTCAATGAACAATCCTGATGCTTTTCTGATTGACAATGTTCCTTCTTCCAATGTATTAGACAGTAGGAACTGTAGCCGGAATTTGGGAGTCACCCTTTCAGAGGTTTCACCGACTTATATGACAGCAGAATCTGGATTTCCTGTCACTTGTCATTCTAGAGCTACTTCTCAGGTCCAGCCTTCCCCTTCTGTGGCTACTGACTGTGTTACAGCAACTCAAATTTCAGAAGAGAACATCTACAATCTTCAAGAAGATACAGTTTTGCCTGAACTAACTGCAATGCAAGGTATTGCCCCACCAAGCACTGTGGAGATCCCTAATGCAAACTTTATGCCCTCTGAGACGGTGAATACAGAGTACATGGATGTGTCAGTTTTGGATCAGGCAATGCCTACAAAAACTGAGGTCTTTTCTCCTGAGATCAATCCTGATGCAGATGTCTCGCAAGATGGAGAGCCTAACCTTCCGGGAATTAATGATGTCTTCTGGGATAAGTTTTTTGACACAGATGAAATTGATTTGAGTTCAGTTGTTGGGGTGACCAAGGATCAGGAGATGCAGTCAGGGAAGGGAAATGGATGGGACAAGATTCAACATATAAGTCCAATTGCTCCTCAGCAAACAGGGCTTGAGGGCATGATTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSF_DNA-bind

Protein Analysis

492

Amino Acids

55.08

Weight (kDa)

5.3

Isoelectric Point (pI)

62.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 17 - 106 5.2e-29 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 98
AccI GTMKAC 1 cut(s) 75
AccII CGCG 1 cut(s) 11
AciI CCGC 5 cut(s) 11, 44, 208, 556, 823
AclWI GGATC 3 cut(s) 1138, 1218, 1386
AcsI RAATTY 4 cut(s) 257, 699, 910, 1050
AcyI GRCGYC 2 cut(s) 114, 144
AfaI GTAC 4 cut(s) 333, 360, 624, 1190
AfiI CCNNNNNNNGG 3 cut(s) 913, 931, 1021
AflIII ACRYGT 1 cut(s) 535
AgsI TTSAA 6 cut(s) 181, 301, 437, 762, 1079, 1424
AjnI CCWGG 1 cut(s) 601
AjuI GAANNNNNNNTTGG 2 cut(s) 110, 142
AluBI AGCT 6 cut(s) 201, 214, 381, 619, 715, 992
AluI AGCT 6 cut(s) 201, 214, 381, 619, 715, 992
Alw21I GWGCWC 1 cut(s) 383
Alw26I GTCTC 4 cut(s) 106, 670, 1166, 1276
AlwI GGATC 3 cut(s) 1138, 1218, 1386
AlwNI CAGNNNCTG 1 cut(s) 962
Ama87I CYCGRG 1 cut(s) 156
ApeKI GCWGC 3 cut(s) 392, 496, 773
ApoI RAATTY 4 cut(s) 257, 699, 910, 1050
ArsI GACNNNNNNTTYG 2 cut(s) 134, 166
AseI ATTAAT 1 cut(s) 1305
Asp700I GAANNNNTTC 1 cut(s) 273
AspA2I CCTAGG 1 cut(s) 818
AspLEI GCGC 1 cut(s) 651
AspS9I GGNCC 3 cut(s) 108, 160, 1003
AsuC2I CCSGG 3 cut(s) 157, 158, 1299
AsuHPI GGTGA 4 cut(s) 913, 930, 1189, 1381
AvaI CYCGRG 1 cut(s) 156
AvaII GGWCC 3 cut(s) 108, 160, 1003
AvrII CCTAGG 1 cut(s) 818
BanII GRGCYC 1 cut(s) 383
BbsI GAAGAC 4 cut(s) 12, 308, 699, 1306
Bbv12I GWGCWC 1 cut(s) 383
BbvCI CCTCAGC 1 cut(s) 1446
BbvI GCAGC 3 cut(s) 379, 508, 785
BccI CCATC 4 cut(s) 246, 728, 1274, 1404
BceAI ACGGC 2 cut(s) 31, 806
BcgI CGANNNNNNTGC 2 cut(s) 1254, 1288
BciT130I CCWGG 1 cut(s) 603
BclI TGATCA 1 cut(s) 21
BcnI CCSGG 3 cut(s) 157, 158, 1299
BcoDI GTCTC 4 cut(s) 106, 670, 1166, 1276
BfaI CTAG 2 cut(s) 819, 987
BfmI CTRYAG 1 cut(s) 901
BisI GCNGC 5 cut(s) 12, 393, 497, 557, 774
BlnI CCTAGG 1 cut(s) 818
BlpI GCTNAGC 1 cut(s) 716
BlsI GCNGC 5 cut(s) 13, 394, 498, 558, 775
Bme1390I CCNGG 4 cut(s) 157, 158, 603, 1299
Bme18I GGWCC 3 cut(s) 108, 160, 1003
BmeT110I CYCGRG 1 cut(s) 156
BmgT120I GGNCC 3 cut(s) 108, 160, 1003
BmiI GGNNCC 2 cut(s) 161, 828
BmrFI CCNGG 4 cut(s) 157, 158, 603, 1299
BmrI ACTGGG 1 cut(s) 142
BmsI GCATC 6 cut(s) 48, 637, 660, 838, 1252, 1377
BmuI ACTGGG 1 cut(s) 142
BpiI GAAGAC 4 cut(s) 12, 308, 699, 1306
BpmI CTGGAG 2 cut(s) 181, 443
Bpu10I CCTNAGC 1 cut(s) 1446
Bpu1102I GCTNAGC 1 cut(s) 716
BpuMI CCSGG 3 cut(s) 157, 158, 1299
BsaHI GRCGYC 2 cut(s) 114, 144
BsaI GGTCTC 1 cut(s) 106
BsaJI CCNNGG 4 cut(s) 156, 683, 818, 1374
BsaXI ACNNNNNCTCC 6 cut(s) 68, 98, 179, 209, 1376, 1406
Bsc4I CCNNNNNNNGG 3 cut(s) 913, 931, 1021
Bse1I ACTGG 5 cut(s) 148, 323, 356, 426, 661
Bse3DI GCAATG 3 cut(s) 789, 1116, 1224
BseBI CCWGG 1 cut(s) 603
BseDI CCNNGG 4 cut(s) 156, 683, 818, 1374
BseGI GGATG 5 cut(s) 39, 241, 739, 1201, 1415
BseLI CCNNNNNNNGG 3 cut(s) 913, 931, 1021
BseMI GCAATG 3 cut(s) 789, 1116, 1224
BseMII CTCAG 5 cut(s) 1013, 1161, 1224, 1239, 1460
BseNI ACTGG 5 cut(s) 148, 323, 356, 426, 661
BseRI GAGGAG 3 cut(s) 440, 443, 1434
BseXI GCAGC 3 cut(s) 379, 508, 785
BseYI CCCAGC 1 cut(s) 615
BsgI GTGCAG 1 cut(s) 560
Bsh1236I CGCG 1 cut(s) 11
BsiHKAI GWGCWC 1 cut(s) 383
BsiHKCI CYCGRG 1 cut(s) 156
BsiSI CCGG 3 cut(s) 157, 907, 1298
BslFI GGGAC 3 cut(s) 173, 464, 1427
BslI CCNNNNNNNGG 3 cut(s) 913, 931, 1021
BsmAI GTCTC 4 cut(s) 106, 670, 1166, 1276
BsmBI CGTCTC 1 cut(s) 1166
BsmFI GGGAC 3 cut(s) 173, 464, 1427
Bso31I GGTCTC 1 cut(s) 106
BsoBI CYCGRG 1 cut(s) 156
Bsp1286I GDGCHC 1 cut(s) 383
Bsp1407I TGTACA 2 cut(s) 331, 622
Bsp143I GATC 5 cut(s) 21, 1143, 1210, 1251, 1378
Bsp1720I GCTNAGC 1 cut(s) 716
BspACI CCGC 5 cut(s) 11, 44, 208, 556, 823
BspCNI CTCAG 5 cut(s) 1012, 1162, 1225, 1240, 1459
BspFNI CGCG 1 cut(s) 11
BspLI GGNNCC 2 cut(s) 161, 828
BspPI GGATC 3 cut(s) 1138, 1218, 1386
BspTNI GGTCTC 1 cut(s) 106
BsrDI GCAATG 3 cut(s) 789, 1116, 1224
BsrGI TGTACA 2 cut(s) 331, 622
BsrI ACTGG 5 cut(s) 148, 323, 356, 426, 661
BssECI CCNNGG 4 cut(s) 156, 683, 818, 1374
BssMI GATC 5 cut(s) 21, 1143, 1210, 1251, 1378
BssNI GRCGYC 2 cut(s) 114, 144
BssT1I CCWWGG 2 cut(s) 818, 1374
Bst2UI CCWGG 1 cut(s) 603
Bst4CI ACNGT 9 cut(s) 125, 307, 831, 893, 902, 1034, 1090, 1138, 1177
Bst6I CTCTTC 1 cut(s) 1053
BstACI GRCGYC 2 cut(s) 114, 144
BstAUI TGTACA 2 cut(s) 331, 622
BstC8I GCNNGC 3 cut(s) 212, 397, 501
BstDEI CTNAG 7 cut(s) 278, 716, 999, 1170, 1233, 1248, 1446
BstEII GGTNACC 1 cut(s) 1369
BstENI CCTNNNNNAGG 1 cut(s) 929
BstF5I GGATG 5 cut(s) 39, 241, 739, 1201, 1415
BstFNI CGCG 1 cut(s) 11
BstHHI GCGC 1 cut(s) 651
BstKTI GATC 5 cut(s) 24, 1146, 1213, 1254, 1381
BstMAI GTCTC 4 cut(s) 106, 670, 1166, 1276
BstMBI GATC 5 cut(s) 21, 1143, 1210, 1251, 1378
BstMWI GCNNNNNNNGC 8 cut(s) 152, 207, 496, 562, 625, 648, 779, 1456
BstNI CCWGG 1 cut(s) 603
BstNSI RCATGY 1 cut(s) 399
BstPI GGTNACC 1 cut(s) 1369
BstSCI CCNGG 4 cut(s) 155, 156, 601, 1297
BstSFI CTRYAG 1 cut(s) 901
BstUI CGCG 1 cut(s) 11
BstV1I GCAGC 3 cut(s) 379, 508, 785
BstV2I GAAGAC 4 cut(s) 12, 308, 699, 1306
BstX2I RGATCY 1 cut(s) 1143
BstXI CCANNNNNNTGG 1 cut(s) 252
BstYI RGATCY 1 cut(s) 1143
BtsCI GGATG 5 cut(s) 39, 241, 739, 1201, 1415
BtsIMutI CAGTG 2 cut(s) 654, 1134
Cac8I GCNNGC 3 cut(s) 212, 397, 501
CaiI CAGNNNCTG 1 cut(s) 962
CfoI GCGC 1 cut(s) 651
Cfr13I GGNCC 3 cut(s) 108, 160, 1003
Cfr9I CCCGGG 1 cut(s) 156
CseI GACGC 3 cut(s) 17, 122, 152
Csp6I GTAC 4 cut(s) 332, 359, 623, 1189
CspCI CAANNNNNGTGG 2 cut(s) 1119, 1154
CviAII CATG 5 cut(s) 70, 105, 396, 1192, 1468
CviQI GTAC 4 cut(s) 332, 359, 623, 1189
DdeI CTNAG 7 cut(s) 278, 716, 999, 1170, 1233, 1248, 1446
DpnI GATC 5 cut(s) 23, 1145, 1212, 1253, 1380
DpnII GATC 5 cut(s) 21, 1143, 1210, 1251, 1378
DrdI GACNNNNNNGTC 1 cut(s) 98
DseDI GACNNNNNNGTC 1 cut(s) 98
Eam1104I CTCTTC 1 cut(s) 1053
EarI CTCTTC 1 cut(s) 1053
EciI GGCGGA 1 cut(s) 197
Ecl136II GAGCTC 1 cut(s) 381
Eco130I CCWWGG 2 cut(s) 818, 1374
Eco24I GRGCYC 1 cut(s) 383
Eco31I GGTCTC 1 cut(s) 106
Eco47I GGWCC 3 cut(s) 108, 160, 1003
Eco53kI GAGCTC 1 cut(s) 381
Eco88I CYCGRG 1 cut(s) 156
Eco91I GGTNACC 1 cut(s) 1369
EcoICRI GAGCTC 1 cut(s) 381
EcoNI CCTNNNNNAGG 1 cut(s) 929
EcoO109I RGGNCCY 1 cut(s) 160
EcoO65I GGTNACC 1 cut(s) 1369
EcoRI GAATTC 1 cut(s) 257
EcoRII CCWGG 1 cut(s) 601
EcoT14I CCWWGG 2 cut(s) 818, 1374
EcoT22I ATGCAT 1 cut(s) 597
EcoT38I GRGCYC 1 cut(s) 383
ErhI CCWWGG 2 cut(s) 818, 1374
Esp3I CGTCTC 1 cut(s) 1166
FaeI CATG 5 cut(s) 73, 108, 399, 1195, 1471
FaqI GGGAC 3 cut(s) 173, 464, 1427
FatI CATG 5 cut(s) 69, 104, 395, 1191, 1467
FauI CCCGC 2 cut(s) 51, 816
FbaI TGATCA 1 cut(s) 21
FblI GTMKAC 1 cut(s) 75
Fnu4HI GCNGC 5 cut(s) 12, 393, 497, 557, 774
FokI GGATG 5 cut(s) 26, 228, 746, 1208, 1422
FriOI GRGCYC 1 cut(s) 383
Fsp4HI GCNGC 5 cut(s) 12, 393, 497, 557, 774
FspBI CTAG 2 cut(s) 819, 987
GlaI GCGC 1 cut(s) 650
GluI GCNGC 5 cut(s) 12, 393, 497, 557, 774
GsaI CCCAGC 1 cut(s) 619
GsuI CTGGAG 2 cut(s) 181, 443
HapII CCGG 3 cut(s) 157, 907, 1298
HgaI GACGC 3 cut(s) 17, 122, 152
HhaI GCGC 1 cut(s) 651
Hin1I GRCGYC 2 cut(s) 114, 144
Hin1II CATG 5 cut(s) 73, 108, 399, 1195, 1471
Hin6I GCGC 1 cut(s) 649
HinP1I GCGC 1 cut(s) 649
HincII GTYRAC 2 cut(s) 76, 533
HindII GTYRAC 2 cut(s) 76, 533
HindIII AAGCTT 1 cut(s) 713
HinfI GANTC 4 cut(s) 337, 918, 959, 1420
HpaII CCGG 3 cut(s) 157, 907, 1298
HphI GGTGA 4 cut(s) 913, 930, 1189, 1381
Hpy166II GTNNAC 2 cut(s) 76, 533
Hpy188I TCNGA 8 cut(s) 54, 288, 633, 684, 858, 931, 1057, 1171
Hpy188III TCNNGA 9 cut(s) 245, 731, 845, 963, 987, 1079, 1247, 1259, 1382
Hpy8I GTNNAC 2 cut(s) 76, 533
Hpy99I CGWCG 6 cut(s) 19, 37, 80, 116, 134, 146
HpyAV CCTTC 7 cut(s) 263, 768, 882, 1020, 1026, 1304, 1393
HpyCH4III ACNGT 9 cut(s) 125, 307, 831, 893, 902, 1034, 1090, 1138, 1177
HpyCH4IV ACGT 1 cut(s) 535
HpyF10VI GCNNNNNNNGC 8 cut(s) 152, 207, 496, 562, 625, 648, 779, 1456
HpyF3I CTNAG 7 cut(s) 278, 716, 999, 1170, 1233, 1248, 1446
HpySE526I ACGT 1 cut(s) 535
Hsp92I GRCGYC 2 cut(s) 114, 144
Hsp92II CATG 5 cut(s) 73, 108, 399, 1195, 1471
HspAI GCGC 1 cut(s) 649
Ksp22I TGATCA 1 cut(s) 21
Kzo9I GATC 5 cut(s) 21, 1143, 1210, 1251, 1378
LmnI GCTCC 3 cut(s) 386, 493, 1447
Lsp1109I GCAGC 3 cut(s) 379, 508, 785
LweI GCATC 6 cut(s) 48, 637, 660, 838, 1252, 1377
MaeI CTAG 2 cut(s) 819, 987
MaeII ACGT 1 cut(s) 535
MaeIII GTNAC 4 cut(s) 919, 973, 1036, 1369
MalI GATC 5 cut(s) 23, 1145, 1212, 1253, 1380
MboI GATC 5 cut(s) 21, 1143, 1210, 1251, 1378
MfeI CAATTG 1 cut(s) 1437
MflI RGATCY 1 cut(s) 1143
MhlI GDGCHC 1 cut(s) 383
MluCI AATT 9 cut(s) 257, 515, 699, 741, 910, 1050, 1302, 1344, 1437
MlyI GAGTC 2 cut(s) 346, 927
Mph1103I ATGCAT 1 cut(s) 597
MroXI GAANNNNTTC 1 cut(s) 273
MseI TTAA 3 cut(s) 327, 444, 1305
MslI CAYNNNNRTG 3 cut(s) 464, 633, 1196
MspA1I CMGCKG 1 cut(s) 556
MspI CCGG 3 cut(s) 157, 907, 1298
MspR9I CCNGG 4 cut(s) 157, 158, 603, 1299
MunI CAATTG 1 cut(s) 1437
MvaI CCWGG 1 cut(s) 603
MvnI CGCG 1 cut(s) 11
MwoI GCNNNNNNNGC 8 cut(s) 152, 207, 496, 562, 625, 648, 779, 1456
NciI CCSGG 3 cut(s) 157, 158, 1299
NdeII GATC 5 cut(s) 21, 1143, 1210, 1251, 1378
NlaIII CATG 5 cut(s) 73, 108, 399, 1195, 1471
NlaIV GGNNCC 2 cut(s) 161, 828
NmeAIII GCCGAG 1 cut(s) 8
NmuCI GTSAC 3 cut(s) 919, 973, 1369
NsiI ATGCAT 1 cut(s) 597
NspI RCATGY 1 cut(s) 399
PaeI GCATGC 1 cut(s) 399
PcsI WCGNNNNNNNCGW 2 cut(s) 72, 138
PdmI GAANNNNTTC 1 cut(s) 273
PfeI GAWTC 2 cut(s) 959, 1420
PflFI GACNNNGTC 1 cut(s) 71
PfoI TCCNGGA 1 cut(s) 1297
PkrI GCNGC 5 cut(s) 13, 394, 498, 558, 775
PleI GAGTC 2 cut(s) 345, 926
PpsI GAGTC 2 cut(s) 345, 926
PpuMI RGGWCCY 1 cut(s) 160
PshBI ATTAAT 1 cut(s) 1305
Psp124BI GAGCTC 1 cut(s) 383
Psp5II RGGWCCY 1 cut(s) 160
Psp6I CCWGG 1 cut(s) 601
PspEI GGTNACC 1 cut(s) 1369
PspFI CCCAGC 1 cut(s) 615
PspGI CCWGG 1 cut(s) 601
PspN4I GGNNCC 2 cut(s) 161, 828
PspPI GGNCC 3 cut(s) 108, 160, 1003
PspPPI RGGWCCY 1 cut(s) 160
PsrI GAACNNNNNNTAC 2 cut(s) 47, 79
PstNI CAGNNNCTG 1 cut(s) 962
PsuI RGATCY 1 cut(s) 1143
PsyI GACNNNGTC 1 cut(s) 71
RsaI GTAC 4 cut(s) 333, 360, 624, 1190
RsaNI GTAC 4 cut(s) 332, 359, 623, 1189
RseI CAYNNNNRTG 3 cut(s) 464, 633, 1196
SacI GAGCTC 1 cut(s) 383
SalI GTCGAC 1 cut(s) 74
SaqAI TTAA 3 cut(s) 327, 444, 1305
SatI GCNGC 5 cut(s) 12, 393, 497, 557, 774
Sau3AI GATC 5 cut(s) 21, 1143, 1210, 1251, 1378
Sau96I GGNCC 3 cut(s) 108, 160, 1003
SchI GAGTC 2 cut(s) 346, 927
ScrFI CCNGG 4 cut(s) 157, 158, 603, 1299
SduI GDGCHC 1 cut(s) 383
SfaNI GCATC 6 cut(s) 48, 637, 660, 838, 1252, 1377
SfcI CTRYAG 1 cut(s) 901
SinI GGWCC 3 cut(s) 108, 160, 1003
SmaI CCCGGG 1 cut(s) 158
SmiMI CAYNNNNRTG 3 cut(s) 464, 633, 1196
SmlI CTYRAG 1 cut(s) 1460
SmoI CTYRAG 1 cut(s) 1460
SphI GCATGC 1 cut(s) 399
Sse9I AATT 9 cut(s) 257, 515, 699, 741, 910, 1050, 1302, 1344, 1437
SsiI CCGC 5 cut(s) 11, 44, 208, 556, 823
SspMI CTAG 2 cut(s) 819, 987
SstI GAGCTC 1 cut(s) 383
StyD4I CCNGG 4 cut(s) 155, 156, 601, 1297
StyI CCWWGG 2 cut(s) 818, 1374
TaaI ACNGT 9 cut(s) 125, 307, 831, 893, 902, 1034, 1090, 1138, 1177
TaiI ACGT 1 cut(s) 538
TaqI TCGA 1 cut(s) 75
TaqII GACCGA 2 cut(s) 125, 264
TasI AATT 9 cut(s) 257, 515, 699, 741, 910, 1050, 1302, 1344, 1437
TatI WGTACW 3 cut(s) 331, 622, 1188
TauI GCSGC 2 cut(s) 14, 559
TfiI GAWTC 2 cut(s) 959, 1420
Tru1I TTAA 3 cut(s) 327, 444, 1305
Tru9I TTAA 3 cut(s) 327, 444, 1305
TscAI CASTG 2 cut(s) 661, 1141
TseFI GTSAC 3 cut(s) 919, 973, 1369
TseI GCWGC 3 cut(s) 392, 496, 773
Tsp45I GTSAC 3 cut(s) 919, 973, 1369
TspDTI ATGAA 8 cut(s) 230, 282, 779, 783, 815, 821, 851, 1356
TspMI CCCGGG 1 cut(s) 156
TspRI CASTG 2 cut(s) 661, 1141
Tth111I GACNNNGTC 1 cut(s) 71
VpaK11BI GGWCC 3 cut(s) 108, 160, 1003
VspI ATTAAT 1 cut(s) 1305
XagI CCTNNNNNAGG 1 cut(s) 929
XapI RAATTY 4 cut(s) 257, 699, 910, 1050
XbaI TCTAGA 1 cut(s) 986
XceI RCATGY 1 cut(s) 399
XcmI CCANNNNNNNNNTGG 1 cut(s) 94
XmaI CCCGGG 1 cut(s) 156
XmaJI CCTAGG 1 cut(s) 818
XmiI GTMKAC 1 cut(s) 75
XmnI GAANNNNTTC 1 cut(s) 273
XspI CTAG 2 cut(s) 819, 987
Zsp2I ATGCAT 1 cut(s) 597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.