Rw5G017370

Heat stress transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0008306
Physical Location & Seq
Reverse (-)
22118537 .. 22121585
3049 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G017370.1

Sequence Viewer

Length: 723 bp
ATGGAAGACGCGGCGGCGATGATCAACTCGGCGACGAGCATCCCGCCGTTTCTGAACAAGACCTACGACATGGTCGACGACCCCTCCACCGACTACTTGGTCTCATGGACCGACGCCAACAACAGCTTCGTCGTTTGGAACGACGCCCAGTTCGCCAGGGACCTTTTGCCCAAGTTCTTCAAGCACAACAACTTCTCCAGCTTCGTCCGCCAGCTCAATACTTACGGTTTTAGAAAAGTTCATCCAGACCGATGGGTTCGCAATGAATTTCAGAGAGAACATCTAAACTTCAAAGAACAATCCCAAGTTGCTCTGAGGGTGAAACTTGGACTGGAGGAGGAGGTTGAAAGGCTTAAAAGGGACAAGAACACTCTTATGCAGGAACTTATTAGTTTGAGGCAGGAGCAGCAAGCAACAGATAAGCAATTACAGAATGTTGGTCAACGTGTGCAGGGAATGGAACAGCGGCAAAAGCAAATGATGTCATTTCTTGCAAAGGCTATGCATACAATGCAACGATTTCTCGGATCTTGTGATTTTGCTTGGAATAATGATGTCTTCTGGGATAAGTTTTTTGACACAGATGAAATTGATTTGAGTTCAGTTGTTGGGGTGACCAAGGATCAGGAGATGCAGTCAGGGAAGGGAAATGGATGGGACAAGATTCAACATATAAGTCCAATTGCTCCTCAGCAAACAGGGCTTGAGGGCATGATTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSF_DNA-bind

Protein Analysis

240

Amino Acids

27.92

Weight (kDa)

5.59

Isoelectric Point (pI)

42.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 17 - 100 2.9e-25 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 98
AccI GTMKAC 1 cut(s) 75
AccII CGCG 1 cut(s) 11
AciI CCGC 5 cut(s) 11, 14, 44, 208, 466
AclWI GGATC 2 cut(s) 535, 630
AcsI RAATTY 1 cut(s) 266
AcyI GRCGYC 2 cut(s) 114, 144
AflIII ACRYGT 1 cut(s) 445
AgsI TTSAA 4 cut(s) 181, 292, 347, 668
AjnI CCWGG 1 cut(s) 155
AjuI GAANNNNNNNTTGG 2 cut(s) 110, 142
AluBI AGCT 3 cut(s) 126, 201, 214
AluI AGCT 3 cut(s) 126, 201, 214
Alw26I GTCTC 1 cut(s) 106
AlwI GGATC 2 cut(s) 535, 630
ApeKI GCWGC 1 cut(s) 406
ApoI RAATTY 1 cut(s) 266
ArsI GACNNNNNNTTYG 2 cut(s) 134, 166
AspS9I GGNCC 2 cut(s) 108, 160
AsuHPI GGTGA 2 cut(s) 331, 625
AvaII GGWCC 2 cut(s) 108, 160
BbsI GAAGAC 2 cut(s) 12, 550
BbvCI CCTCAGC 1 cut(s) 690
BbvI GCAGC 1 cut(s) 418
BccI CCATC 2 cut(s) 246, 648
BceAI ACGGC 1 cut(s) 31
BciT130I CCWGG 1 cut(s) 157
BclI TGATCA 1 cut(s) 21
BcoDI GTCTC 1 cut(s) 106
BisI GCNGC 4 cut(s) 12, 15, 407, 467
BlsI GCNGC 4 cut(s) 13, 16, 408, 468
Bme1390I CCNGG 1 cut(s) 157
Bme18I GGWCC 2 cut(s) 108, 160
BmgT120I GGNCC 2 cut(s) 108, 160
BmiI GGNNCC 1 cut(s) 161
BmrFI CCNGG 1 cut(s) 157
BmrI ACTGGG 1 cut(s) 142
BmsI GCATC 2 cut(s) 48, 621
BmuI ACTGGG 1 cut(s) 142
BpiI GAAGAC 2 cut(s) 12, 550
BpmI CTGGAG 2 cut(s) 181, 353
Bpu10I CCTNAGC 1 cut(s) 690
BsaHI GRCGYC 2 cut(s) 114, 144
BsaI GGTCTC 1 cut(s) 106
BsaJI CCNNGG 2 cut(s) 156, 618
BsaXI ACNNNNNCTCC 6 cut(s) 68, 98, 179, 209, 620, 650
Bse1I ACTGG 2 cut(s) 148, 336
Bse3DI GCAATG 1 cut(s) 268
BseBI CCWGG 1 cut(s) 157
BseDI CCNNGG 2 cut(s) 156, 618
BseGI GGATG 3 cut(s) 39, 241, 659
BseMI GCAATG 1 cut(s) 268
BseMII CTCAG 2 cut(s) 305, 704
BseNI ACTGG 2 cut(s) 148, 336
BseRI GAGGAG 3 cut(s) 350, 353, 678
BseXI GCAGC 1 cut(s) 418
BsgI GTGCAG 1 cut(s) 470
Bsh1236I CGCG 1 cut(s) 11
BslFI GGGAC 3 cut(s) 173, 374, 671
BsmAI GTCTC 1 cut(s) 106
BsmFI GGGAC 3 cut(s) 173, 374, 671
Bso31I GGTCTC 1 cut(s) 106
Bsp143I GATC 3 cut(s) 21, 527, 622
BspACI CCGC 5 cut(s) 11, 14, 44, 208, 466
BspCNI CTCAG 2 cut(s) 306, 703
BspFNI CGCG 1 cut(s) 11
BspLI GGNNCC 1 cut(s) 161
BspPI GGATC 2 cut(s) 535, 630
BspTNI GGTCTC 1 cut(s) 106
BsrDI GCAATG 1 cut(s) 268
BsrI ACTGG 2 cut(s) 148, 336
BssECI CCNNGG 2 cut(s) 156, 618
BssMI GATC 3 cut(s) 21, 527, 622
BssNI GRCGYC 2 cut(s) 114, 144
BssT1I CCWWGG 1 cut(s) 618
Bst2UI CCWGG 1 cut(s) 157
Bst4CI ACNGT 1 cut(s) 227
BstACI GRCGYC 2 cut(s) 114, 144
BstAPI GCANNNNNTGC 1 cut(s) 511
BstC8I GCNNGC 2 cut(s) 212, 411
BstDEI CTNAG 2 cut(s) 314, 690
BstEII GGTNACC 1 cut(s) 613
BstF5I GGATG 3 cut(s) 39, 241, 659
BstFNI CGCG 1 cut(s) 11
BstKTI GATC 3 cut(s) 24, 530, 625
BstMAI GTCTC 1 cut(s) 106
BstMBI GATC 3 cut(s) 21, 527, 622
BstMWI GCNNNNNNNGC 6 cut(s) 152, 207, 406, 472, 511, 700
BstNI CCWGG 1 cut(s) 157
BstPI GGTNACC 1 cut(s) 613
BstSCI CCNGG 1 cut(s) 155
BstUI CGCG 1 cut(s) 11
BstV1I GCAGC 1 cut(s) 418
BstV2I GAAGAC 2 cut(s) 12, 550
BstX2I RGATCY 1 cut(s) 527
BstXI CCANNNNNNTGG 1 cut(s) 252
BstYI RGATCY 1 cut(s) 527
BtgZI GCGATG 1 cut(s) 32
BtsCI GGATG 3 cut(s) 39, 241, 659
Cac8I GCNNGC 2 cut(s) 212, 411
Cfr13I GGNCC 2 cut(s) 108, 160
CseI GACGC 3 cut(s) 17, 122, 152
CviAII CATG 3 cut(s) 70, 105, 712
CviJI RGCY 6 cut(s) 126, 201, 214, 352, 500, 703
CviKI_1 RGCY 6 cut(s) 126, 201, 214, 352, 500, 703
DdeI CTNAG 2 cut(s) 314, 690
DpnI GATC 3 cut(s) 23, 529, 624
DpnII GATC 3 cut(s) 21, 527, 622
DrdI GACNNNNNNGTC 1 cut(s) 98
DseDI GACNNNNNNGTC 1 cut(s) 98
EciI GGCGGA 1 cut(s) 197
Eco130I CCWWGG 1 cut(s) 618
Eco31I GGTCTC 1 cut(s) 106
Eco47I GGWCC 2 cut(s) 108, 160
Eco91I GGTNACC 1 cut(s) 613
EcoO109I RGGNCCY 1 cut(s) 160
EcoO65I GGTNACC 1 cut(s) 613
EcoRII CCWGG 1 cut(s) 155
EcoT14I CCWWGG 1 cut(s) 618
EcoT22I ATGCAT 1 cut(s) 507
ErhI CCWWGG 1 cut(s) 618
FaeI CATG 3 cut(s) 73, 108, 715
FaiI YATR 8 cut(s) 71, 106, 377, 503, 507, 672, 674, 713
FaqI GGGAC 3 cut(s) 173, 374, 671
FatI CATG 3 cut(s) 69, 104, 711
FauI CCCGC 1 cut(s) 51
FbaI TGATCA 1 cut(s) 21
FblI GTMKAC 1 cut(s) 75
Fnu4HI GCNGC 4 cut(s) 12, 15, 407, 467
FokI GGATG 3 cut(s) 26, 228, 666
Fsp4HI GCNGC 4 cut(s) 12, 15, 407, 467
GluI GCNGC 4 cut(s) 12, 15, 407, 467
GsuI CTGGAG 2 cut(s) 181, 353
HgaI GACGC 3 cut(s) 17, 122, 152
Hin1I GRCGYC 2 cut(s) 114, 144
Hin1II CATG 3 cut(s) 73, 108, 715
HincII GTYRAC 2 cut(s) 76, 443
HindII GTYRAC 2 cut(s) 76, 443
HinfI GANTC 1 cut(s) 664
HphI GGTGA 2 cut(s) 331, 625
Hpy166II GTNNAC 2 cut(s) 76, 443
Hpy188I TCNGA 4 cut(s) 54, 273, 315, 527
Hpy188III TCNNGA 2 cut(s) 245, 626
Hpy8I GTNNAC 2 cut(s) 76, 443
Hpy99I CGWCG 5 cut(s) 37, 80, 116, 134, 146
HpyAV CCTTC 1 cut(s) 637
HpyCH4III ACNGT 1 cut(s) 227
HpyCH4IV ACGT 1 cut(s) 445
HpyCH4V TGCA 6 cut(s) 379, 451, 494, 505, 514, 634
HpyF10VI GCNNNNNNNGC 6 cut(s) 152, 207, 406, 472, 511, 700
HpyF3I CTNAG 2 cut(s) 314, 690
HpySE526I ACGT 1 cut(s) 445
Hsp92I GRCGYC 2 cut(s) 114, 144
Hsp92II CATG 3 cut(s) 73, 108, 715
Ksp22I TGATCA 1 cut(s) 21
Kzo9I GATC 3 cut(s) 21, 527, 622
LmnI GCTCC 2 cut(s) 403, 691
Lsp1109I GCAGC 1 cut(s) 418
LweI GCATC 2 cut(s) 48, 621
MaeII ACGT 1 cut(s) 445
MaeIII GTNAC 1 cut(s) 613
MalI GATC 3 cut(s) 23, 529, 624
MboI GATC 3 cut(s) 21, 527, 622
MboII GAAGA 3 cut(s) 17, 169, 550
MfeI CAATTG 1 cut(s) 681
MflI RGATCY 1 cut(s) 527
MluCI AATT 4 cut(s) 266, 425, 588, 681
MnlI CCTC 8 cut(s) 94, 309, 328, 331, 334, 390, 699, 700
Mph1103I ATGCAT 1 cut(s) 507
MseI TTAA 1 cut(s) 354
MslI CAYNNNNRTG 1 cut(s) 374
MspA1I CMGCKG 1 cut(s) 466
MspR9I CCNGG 1 cut(s) 157
MunI CAATTG 1 cut(s) 681
MvaI CCWGG 1 cut(s) 157
MvnI CGCG 1 cut(s) 11
MwoI GCNNNNNNNGC 6 cut(s) 152, 207, 406, 472, 511, 700
NdeII GATC 3 cut(s) 21, 527, 622
NlaIII CATG 3 cut(s) 73, 108, 715
NlaIV GGNNCC 1 cut(s) 161
NmeAIII GCCGAG 1 cut(s) 8
NmuCI GTSAC 1 cut(s) 613
NsiI ATGCAT 1 cut(s) 507
PcsI WCGNNNNNNNCGW 2 cut(s) 72, 138
PfeI GAWTC 1 cut(s) 664
PflFI GACNNNGTC 1 cut(s) 71
PkrI GCNGC 4 cut(s) 13, 16, 408, 468
PpuMI RGGWCCY 1 cut(s) 160
Psp5II RGGWCCY 1 cut(s) 160
Psp6I CCWGG 1 cut(s) 155
PspEI GGTNACC 1 cut(s) 613
PspGI CCWGG 1 cut(s) 155
PspN4I GGNNCC 1 cut(s) 161
PspPI GGNCC 2 cut(s) 108, 160
PspPPI RGGWCCY 1 cut(s) 160
PsrI GAACNNNNNNTAC 2 cut(s) 47, 79
PsuI RGATCY 1 cut(s) 527
PsyI GACNNNGTC 1 cut(s) 71
RseI CAYNNNNRTG 1 cut(s) 374
SalI GTCGAC 1 cut(s) 74
SaqAI TTAA 1 cut(s) 354
SatI GCNGC 4 cut(s) 12, 15, 407, 467
Sau3AI GATC 3 cut(s) 21, 527, 622
Sau96I GGNCC 2 cut(s) 108, 160
ScrFI CCNGG 1 cut(s) 157
SetI ASST 7 cut(s) 65, 128, 165, 203, 216, 345, 448
SfaNI GCATC 2 cut(s) 48, 621
SinI GGWCC 2 cut(s) 108, 160
SmiMI CAYNNNNRTG 1 cut(s) 374
SmlI CTYRAG 1 cut(s) 704
SmoI CTYRAG 1 cut(s) 704
Sse9I AATT 4 cut(s) 266, 425, 588, 681
SsiI CCGC 5 cut(s) 11, 14, 44, 208, 466
StyD4I CCNGG 1 cut(s) 155
StyI CCWWGG 1 cut(s) 618
TaaI ACNGT 1 cut(s) 227
TaiI ACGT 1 cut(s) 448
TaqI TCGA 1 cut(s) 75
TaqII GACCGA 2 cut(s) 125, 264
TasI AATT 4 cut(s) 266, 425, 588, 681
TauI GCSGC 3 cut(s) 14, 17, 469
TfiI GAWTC 1 cut(s) 664
Tru1I TTAA 1 cut(s) 354
Tru9I TTAA 1 cut(s) 354
TseFI GTSAC 1 cut(s) 613
TseI GCWGC 1 cut(s) 406
Tsp45I GTSAC 1 cut(s) 613
TspDTI ATGAA 3 cut(s) 230, 279, 600
Tth111I GACNNNGTC 1 cut(s) 71
VpaK11BI GGWCC 2 cut(s) 108, 160
XapI RAATTY 1 cut(s) 266
XcmI CCANNNNNNNNNTGG 1 cut(s) 94
XmiI GTMKAC 1 cut(s) 75
Zsp2I ATGCAT 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.