MD10G1210600.v1.1

Belongs to the phosphoglycerate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008242
Physical Location & Seq
Forward (+)
31007478 .. 31010767
3290 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1210600.v1.1.491

Sequence Viewer

Length: 1137 bp
ATGACAACTCCAACATCACTGACGATACTAGAATCCGCGCCGCCGTGCCCACCATCAAGTACTTGCTCGGCCATGGCGCCAAAGTTATCCTTGCTTCTCACCTGTGGGATGGATTTGTGTTGCAAGGGACGTCCCAAGGGAGTCACTCCCAAGTACAGTTTGAAGCCTCTCGTGCCCAGACTTTCTGAGCTCCTTGGACTCGAGGTTAAGATTGCCAATGACTGTATTGGTGAGGAAGTCGAGAAATTGGTTGCTCAACTTCCAGAGGGAGGAGTTTTGCTCCTTGAGAATGTTAGGTTCTACAAGGAGGAAGAGAAGAATGACCCTGAATTCGCCAAGAAGCTTGCTTCACTTGCAGATGTTTATGTGAATGATGCTTTTGGTACTGCTCATAGGGCTCATGCATCAACAGAGGGATTGGCTAAGTACCTGAAGCCTTCCGTAGCTGGATTCCTTATGCAGAAGGAACTCGACTATCTTGTCGGTGCCGTGTCAAATCCTAAGAGGCCATTTGCTGCTATTGTTGGCGGTTCAAAAGTGTCAACCAAGATTGGAGTGATAGAATCCTTGTTGGCGAAGGTTAACGTTCTCTTGCTTGGTGGAGGAATGATCTTTACTTTCTACAAGGCCCAAGGCCATTCAGTTGGATCTTCCCTTGTGGAGGAGGACAAGCTTGATCTTGCAAAGTCACTTCTTGAGAAGGCCAAGTCCAAGGGGGTTTCTATTCTCCTCCCAACTGATGTGGTTATTGCTGACAAGTTTGCTGCTGATGCAAACAGCAAGGTTGTGCCAGCGTCTGCTATTCCAGATGGTTGGATGGGATTGGATATTGGACCAGACTCGATCAAAACTTTCAGTGAAGCTCTCGATACCACTCAAACTATTATCTGGAACGGACCTATGGGTGTTTTCGAATTTGAGAAGTTTGCTGCTGGGACTGAGGCAATAGCTAAGAAGCTTGCAGAGCTGAGCGGCAAGGGCGTGACAACAATAATCGGAGGTGGTGACTCAGTCGCCGCCGTTGAGAAGGCTGGGCTTGCTGAGAAGATGAGCCACATCTCCACCGGAGGCGGTGCAAGCTTAGAGCTCCTCGAAGGGAAAACACTACCCGGAGTCCTTGCTCTCGACGATGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002237 GO:0003674 GO:0003824 GO:0004618 GO:0004672 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0005911 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006464 GO:0006468 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009266 GO:0009314 GO:0009408 GO:0009416 GO:0009506 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009743 GO:0009746 GO:0009749 GO:0009987 GO:0010033 GO:0016020 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016774 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019538 GO:0019637 GO:0019693 GO:0019752 GO:0030054 GO:0031090 GO:0032787 GO:0034284 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0036211 GO:0042221 GO:0042866 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044267 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0048046 GO:0050896 GO:0051186 GO:0051188 GO:0051704 GO:0051707 GO:0055044 GO:0055086 GO:0071704 GO:0071944 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:0098588 GO:0098805 GO:0140096 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00010 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
PGK

Protein Analysis

379

Amino Acids

39.55

Weight (kDa)

5.48

Isoelectric Point (pI)

29.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGK PF00162 43 - 367 4.3e-142 Phosphoglycerate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 479
AatII GACGTC 1 cut(s) 133
AccB1I GGYRCC 2 cut(s) 76, 485
AccBSI CCGCTC 1 cut(s) 972
AccII CGCG 1 cut(s) 38
AciI CCGC 6 cut(s) 36, 41, 528, 972, 1017, 1071
AclI AACGTT 1 cut(s) 585
AclWI GGATC 1 cut(s) 655
AcoI YGGCCR 1 cut(s) 69
AcsI RAATTY 2 cut(s) 329, 914
AcuI CTGAAG 1 cut(s) 452
AcyI GRCGYC 2 cut(s) 77, 130
AfaI GTAC 4 cut(s) 61, 155, 385, 428
AfiI CCNNNNNNNGG 2 cut(s) 269, 661
AgsI TTSAA 2 cut(s) 163, 534
Alw21I GWGCWC 2 cut(s) 192, 1089
AlwI GGATC 1 cut(s) 655
AlwNI CAGNNNCTG 1 cut(s) 797
Ama87I CYCGRG 1 cut(s) 200
AoxI GGCC 5 cut(s) 69, 506, 627, 634, 702
ApeKI GCWGC 3 cut(s) 515, 764, 929
ApoI RAATTY 2 cut(s) 329, 914
ArsI GACNNNNNNTTYG 2 cut(s) 314, 346
AspLEI GCGC 2 cut(s) 40, 79
AspS9I GGNCC 3 cut(s) 628, 833, 896
AsuC2I CCSGG 1 cut(s) 1110
AsuHPI GGTGA 3 cut(s) 91, 242, 1016
AsuII TTCGAA 1 cut(s) 912
AvaI CYCGRG 1 cut(s) 200
AvaII GGWCC 2 cut(s) 833, 896
BaeGI GKGCMC 2 cut(s) 50, 177
BanI GGYRCC 2 cut(s) 76, 485
BanII GRGCYC 3 cut(s) 192, 400, 1089
BauI CACGAG 1 cut(s) 170
Bbv12I GWGCWC 2 cut(s) 192, 1089
BbvI GCAGC 3 cut(s) 502, 751, 916
BccI CCATC 4 cut(s) 61, 103, 803, 811
BceAI ACGGC 3 cut(s) 28, 473, 1004
BcnI CCSGG 1 cut(s) 1110
BfaI CTAG 1 cut(s) 29
BfoI RGCGCY 1 cut(s) 80
BisI GCNGC 6 cut(s) 41, 516, 765, 930, 973, 1017
BlpI GCTNAGC 1 cut(s) 968
BlsI GCNGC 6 cut(s) 42, 517, 766, 931, 974, 1018
BmcAI AGTACT 1 cut(s) 61
Bme1390I CCNGG 1 cut(s) 1110
Bme18I GGWCC 2 cut(s) 833, 896
BmeT110I CYCGRG 1 cut(s) 200
BmgT120I GGNCC 3 cut(s) 628, 833, 896
BmiI GGNNCC 2 cut(s) 78, 487
BmrFI CCNGG 1 cut(s) 1110
BmsI GCATC 4 cut(s) 364, 413, 760, 1120
BplI GAGNNNNNCTC 2 cut(s) 264, 296
Bpu1102I GCTNAGC 1 cut(s) 968
Bpu14I TTCGAA 1 cut(s) 912
BpuEI CTTGAG 2 cut(s) 305, 716
BpuMI CCSGG 1 cut(s) 1110
BsaHI GRCGYC 2 cut(s) 77, 130
BsaJI CCNNGG 5 cut(s) 72, 135, 193, 631, 711
BsaWI WCCGGW 1 cut(s) 1064
Bsc4I CCNNNNNNNGG 2 cut(s) 269, 661
BseDI CCNNGG 5 cut(s) 72, 135, 193, 631, 711
BseGI GGATG 2 cut(s) 114, 822
BseLI CCNNNNNNNGG 2 cut(s) 269, 661
BseMII CTCAG 5 cut(s) 177, 930, 959, 1023, 1032
BseRI GAGGAG 4 cut(s) 285, 677, 719, 1079
BseSI GKGCMC 2 cut(s) 50, 177
BseXI GCAGC 3 cut(s) 502, 751, 916
BseYI CCCAGC 2 cut(s) 932, 1031
Bsh1236I CGCG 1 cut(s) 38
BshFI GGCC 5 cut(s) 71, 508, 629, 636, 704
BshNI GGYRCC 2 cut(s) 76, 485
BsiHKAI GWGCWC 2 cut(s) 192, 1089
BsiHKCI CYCGRG 1 cut(s) 200
BsiSI CCGG 2 cut(s) 1065, 1110
BslFI GGGAC 3 cut(s) 117, 141, 949
BslI CCNNNNNNNGG 2 cut(s) 269, 661
BsmFI GGGAC 3 cut(s) 117, 141, 949
BsnI GGCC 5 cut(s) 71, 508, 629, 636, 704
BsoBI CYCGRG 1 cut(s) 200
Bsp119I TTCGAA 1 cut(s) 912
Bsp1286I GDGCHC 5 cut(s) 50, 177, 192, 400, 1089
Bsp143I GATC 4 cut(s) 609, 647, 676, 843
Bsp1720I GCTNAGC 1 cut(s) 968
Bsp19I CCATGG 1 cut(s) 72
BspACI CCGC 6 cut(s) 36, 41, 528, 972, 1017, 1071
BspANI GGCC 5 cut(s) 71, 508, 629, 636, 704
BspCNI CTCAG 5 cut(s) 178, 931, 960, 1022, 1033
BspFNI CGCG 1 cut(s) 38
BspLI GGNNCC 2 cut(s) 78, 487
BspPI GGATC 1 cut(s) 655
BspT104I TTCGAA 1 cut(s) 912
BspT107I GGYRCC 2 cut(s) 76, 485
BsrBI CCGCTC 1 cut(s) 972
BssECI CCNNGG 5 cut(s) 72, 135, 193, 631, 711
BssMI GATC 4 cut(s) 609, 647, 676, 843
BssNI GRCGYC 2 cut(s) 77, 130
BssSI CACGAG 1 cut(s) 170
BssT1I CCWWGG 5 cut(s) 72, 135, 193, 631, 711
Bst2BI CACGAG 1 cut(s) 170
Bst4CI ACNGT 2 cut(s) 158, 224
Bst6I CTCTTC 1 cut(s) 306
BstACI GRCGYC 2 cut(s) 77, 130
BstBI TTCGAA 1 cut(s) 912
BstC8I GCNNGC 5 cut(s) 345, 792, 960, 1038, 1078
BstDEI CTNAG 9 cut(s) 186, 423, 501, 939, 951, 968, 1009, 1041, 1081
BstDSI CCRYGG 1 cut(s) 72
BstENI CCTNNNNNAGG 1 cut(s) 659
BstF5I GGATG 2 cut(s) 114, 822
BstFNI CGCG 1 cut(s) 38
BstH2I RGCGCY 1 cut(s) 80
BstHHI GCGC 2 cut(s) 40, 79
BstKTI GATC 4 cut(s) 612, 650, 679, 846
BstMBI GATC 4 cut(s) 609, 647, 676, 843
BstMWI GCNNNNNNNGC 8 cut(s) 172, 353, 395, 770, 964, 978, 1037, 1077
BstSCI CCNGG 1 cut(s) 1108
BstSLI GKGCMC 2 cut(s) 50, 177
BstUI CGCG 1 cut(s) 38
BstV1I GCAGC 3 cut(s) 502, 751, 916
BstX2I RGATCY 1 cut(s) 647
BstXI CCANNNNNNTGG 2 cut(s) 644, 813
BstYI RGATCY 1 cut(s) 647
BsuRI GGCC 5 cut(s) 71, 508, 629, 636, 704
BtgI CCRYGG 1 cut(s) 72
BtsCI GGATG 2 cut(s) 114, 822
BtsIMutI CAGTG 2 cut(s) 17, 862
Cac8I GCNNGC 5 cut(s) 345, 792, 960, 1038, 1078
CaiI CAGNNNCTG 1 cut(s) 797
CfoI GCGC 2 cut(s) 40, 79
Cfr13I GGNCC 3 cut(s) 628, 833, 896
CseI GACGC 1 cut(s) 783
Csp6I GTAC 4 cut(s) 60, 154, 384, 427
CspCI CAANNNNNGTGG 2 cut(s) 723, 758
CviAII CATG 2 cut(s) 73, 401
CviQI GTAC 4 cut(s) 60, 154, 384, 427
DdeI CTNAG 9 cut(s) 186, 423, 501, 939, 951, 968, 1009, 1041, 1081
DinI GGCGCC 1 cut(s) 78
DpnI GATC 4 cut(s) 611, 649, 678, 845
DpnII GATC 4 cut(s) 609, 647, 676, 843
DrdI GACNNNNNNGTC 1 cut(s) 479
DseDI GACNNNNNNGTC 1 cut(s) 479
EaeI YGGCCR 1 cut(s) 69
Eam1104I CTCTTC 1 cut(s) 306
EarI CTCTTC 1 cut(s) 306
Ecl136II GAGCTC 2 cut(s) 190, 1087
Eco130I CCWWGG 5 cut(s) 72, 135, 193, 631, 711
Eco24I GRGCYC 3 cut(s) 192, 400, 1089
Eco47I GGWCC 2 cut(s) 833, 896
Eco53kI GAGCTC 2 cut(s) 190, 1087
Eco57I CTGAAG 1 cut(s) 452
Eco88I CYCGRG 1 cut(s) 200
EcoICRI GAGCTC 2 cut(s) 190, 1087
EcoNI CCTNNNNNAGG 1 cut(s) 659
EcoRI GAATTC 1 cut(s) 329
EcoT14I CCWWGG 5 cut(s) 72, 135, 193, 631, 711
EcoT22I ATGCAT 1 cut(s) 406
EcoT38I GRGCYC 3 cut(s) 192, 400, 1089
EgeI GGCGCC 1 cut(s) 78
EheI GGCGCC 1 cut(s) 78
ErhI CCWWGG 5 cut(s) 72, 135, 193, 631, 711
FaeI CATG 2 cut(s) 76, 404
FaiI YATR 6 cut(s) 74, 366, 393, 402, 458, 902
FalI AAGNNNNNCTT 2 cut(s) 74, 106
FaqI GGGAC 3 cut(s) 117, 141, 949
FatI CATG 2 cut(s) 72, 400
Fnu4HI GCNGC 6 cut(s) 41, 516, 765, 930, 973, 1017
FokI GGATG 2 cut(s) 121, 829
FriOI GRGCYC 3 cut(s) 192, 400, 1089
Fsp4HI GCNGC 6 cut(s) 41, 516, 765, 930, 973, 1017
FspBI CTAG 1 cut(s) 29
GlaI GCGC 2 cut(s) 39, 78
GluI GCNGC 6 cut(s) 41, 516, 765, 930, 973, 1017
GsaI CCCAGC 2 cut(s) 936, 1035
HaeII RGCGCY 1 cut(s) 80
HaeIII GGCC 5 cut(s) 71, 508, 629, 636, 704
HapII CCGG 2 cut(s) 1065, 1110
HgaI GACGC 1 cut(s) 783
HhaI GCGC 2 cut(s) 40, 79
Hin1I GRCGYC 2 cut(s) 77, 130
Hin1II CATG 2 cut(s) 76, 404
Hin6I GCGC 2 cut(s) 38, 77
HinP1I GCGC 2 cut(s) 38, 77
HincII GTYRAC 2 cut(s) 543, 583
HindII GTYRAC 2 cut(s) 543, 583
HindIII AAGCTT 4 cut(s) 341, 671, 956, 1078
HinfI GANTC 8 cut(s) 32, 141, 198, 450, 563, 839, 1007, 1113
HpaI GTTAAC 1 cut(s) 583
HpaII CCGG 2 cut(s) 1065, 1110
HphI GGTGA 3 cut(s) 91, 242, 1016
Hpy166II GTNNAC 2 cut(s) 543, 583
Hpy188I TCNGA 2 cut(s) 187, 998
Hpy188III TCNNGA 7 cut(s) 241, 263, 695, 806, 866, 889, 1124
Hpy8I GTNNAC 2 cut(s) 543, 583
Hpy99I CGWCG 1 cut(s) 1130
HpyAV CCTTC 6 cut(s) 447, 457, 571, 694, 1021, 1088
HpyCH4III ACNGT 2 cut(s) 158, 224
HpyCH4IV ACGT 2 cut(s) 130, 585
HpyCH4V TGCA 8 cut(s) 123, 356, 404, 460, 683, 773, 962, 1076
HpyF10VI GCNNNNNNNGC 8 cut(s) 172, 353, 395, 770, 964, 978, 1037, 1077
HpyF3I CTNAG 9 cut(s) 186, 423, 501, 939, 951, 968, 1009, 1041, 1081
HpySE526I ACGT 2 cut(s) 130, 585
Hsp92I GRCGYC 2 cut(s) 77, 130
Hsp92II CATG 2 cut(s) 76, 404
HspAI GCGC 2 cut(s) 38, 77
KasI GGCGCC 1 cut(s) 76
KspAI GTTAAC 1 cut(s) 583
Kzo9I GATC 4 cut(s) 609, 647, 676, 843
LmnI GCTCC 3 cut(s) 195, 285, 1092
Lsp1109I GCAGC 3 cut(s) 502, 751, 916
LweI GCATC 4 cut(s) 364, 413, 760, 1120
MaeI CTAG 1 cut(s) 29
MaeII ACGT 2 cut(s) 130, 585
MaeIII GTNAC 4 cut(s) 142, 687, 982, 1004
MalI GATC 4 cut(s) 611, 649, 678, 845
MbiI CCGCTC 1 cut(s) 972
MboI GATC 4 cut(s) 609, 647, 676, 843
MboII GAAGA 4 cut(s) 323, 328, 642, 1057
MflI RGATCY 1 cut(s) 647
MhlI GDGCHC 5 cut(s) 50, 177, 192, 400, 1089
MluCI AATT 3 cut(s) 245, 329, 914
Mly113I GGCGCC 1 cut(s) 77
MlyI GAGTC 5 cut(s) 150, 192, 833, 1001, 1122
MmeI TCCRAC 3 cut(s) 35, 625, 794
Mph1103I ATGCAT 1 cut(s) 406
MseI TTAA 3 cut(s) 207, 582, 1135
MspI CCGG 2 cut(s) 1065, 1110
MspR9I CCNGG 1 cut(s) 1110
MvnI CGCG 1 cut(s) 38
MwoI GCNNNNNNNGC 8 cut(s) 172, 353, 395, 770, 964, 978, 1037, 1077
NarI GGCGCC 1 cut(s) 77
NciI CCSGG 1 cut(s) 1110
NcoI CCATGG 1 cut(s) 72
NdeII GATC 4 cut(s) 609, 647, 676, 843
NlaIII CATG 2 cut(s) 76, 404
NlaIV GGNNCC 2 cut(s) 78, 487
NmeAIII GCCGAG 1 cut(s) 47
NmuCI GTSAC 4 cut(s) 142, 687, 982, 1004
NsiI ATGCAT 1 cut(s) 406
NspV TTCGAA 1 cut(s) 912
PaeR7I CTCGAG 1 cut(s) 200
PfeI GAWTC 3 cut(s) 32, 450, 563
PflFI GACNNNGTC 1 cut(s) 1010
PkrI GCNGC 6 cut(s) 42, 517, 766, 931, 974, 1018
PleI GAGTC 5 cut(s) 149, 192, 833, 1001, 1121
PluTI GGCGCC 1 cut(s) 80
PpsI GAGTC 5 cut(s) 149, 192, 833, 1001, 1121
Psp124BI GAGCTC 2 cut(s) 192, 1089
Psp1406I AACGTT 1 cut(s) 585
PspFI CCCAGC 2 cut(s) 932, 1031
PspN4I GGNNCC 2 cut(s) 78, 487
PspPI GGNCC 3 cut(s) 628, 833, 896
PspXI VCTCGAGB 1 cut(s) 200
PstNI CAGNNNCTG 1 cut(s) 797
PsuI RGATCY 1 cut(s) 647
PsyI GACNNNGTC 1 cut(s) 1010
RsaI GTAC 4 cut(s) 61, 155, 385, 428
RsaNI GTAC 4 cut(s) 60, 154, 384, 427
SacI GAGCTC 2 cut(s) 192, 1089
SaqAI TTAA 3 cut(s) 207, 582, 1135
SatI GCNGC 6 cut(s) 41, 516, 765, 930, 973, 1017
Sau3AI GATC 4 cut(s) 609, 647, 676, 843
Sau96I GGNCC 3 cut(s) 628, 833, 896
ScaI AGTACT 1 cut(s) 61
SchI GAGTC 5 cut(s) 150, 192, 833, 1001, 1122
ScrFI CCNGG 1 cut(s) 1110
SduI GDGCHC 5 cut(s) 50, 177, 192, 400, 1089
SfaNI GCATC 4 cut(s) 364, 413, 760, 1120
SfoI GGCGCC 1 cut(s) 78
Sfr274I CTCGAG 1 cut(s) 200
SfuI TTCGAA 1 cut(s) 912
SinI GGWCC 2 cut(s) 833, 896
SlaI CTCGAG 1 cut(s) 200
SmlI CTYRAG 3 cut(s) 200, 284, 695
SmoI CTYRAG 3 cut(s) 200, 284, 695
Sse9I AATT 3 cut(s) 245, 329, 914
SsiI CCGC 6 cut(s) 36, 41, 528, 972, 1017, 1071
SspDI GGCGCC 1 cut(s) 76
SspMI CTAG 1 cut(s) 29
SstI GAGCTC 2 cut(s) 192, 1089
StyD4I CCNGG 1 cut(s) 1108
StyI CCWWGG 5 cut(s) 72, 135, 193, 631, 711
TaaI ACNGT 2 cut(s) 158, 224
TaiI ACGT 2 cut(s) 133, 588
TaqI TCGA 8 cut(s) 201, 240, 471, 842, 867, 912, 1092, 1125
TasI AATT 3 cut(s) 245, 329, 914
TatI WGTACW 2 cut(s) 59, 153
TauI GCSGC 3 cut(s) 43, 975, 1019
TfiI GAWTC 3 cut(s) 32, 450, 563
Tru1I TTAA 3 cut(s) 207, 582, 1135
Tru9I TTAA 3 cut(s) 207, 582, 1135
TscAI CASTG 2 cut(s) 24, 862
TseFI GTSAC 4 cut(s) 142, 687, 982, 1004
TseI GCWGC 3 cut(s) 515, 764, 929
Tsp45I GTSAC 4 cut(s) 142, 687, 982, 1004
TspGWI ACGGA 2 cut(s) 430, 909
TspRI CASTG 2 cut(s) 24, 862
Tth111I GACNNNGTC 1 cut(s) 1010
VpaK11BI GGWCC 2 cut(s) 833, 896
XagI CCTNNNNNAGG 1 cut(s) 659
XapI RAATTY 2 cut(s) 329, 914
XhoI CTCGAG 1 cut(s) 200
XspI CTAG 1 cut(s) 29
ZraI GACGTC 1 cut(s) 131
ZrmI AGTACT 1 cut(s) 61
Zsp2I ATGCAT 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.