Rh5DG174700

Belongs to the phosphoglycerate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0008242
Physical Location & Seq
Forward (+)
18712228 .. 18715662
3435 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG174700.1

Sequence Viewer

Length: 846 bp
ATGTCGTGTTTACAGGGACGTCCAAAGGGTGTCACTCCCAAGTACAGTTTGAAGCCTCTTGTGCCAAGACTGTCTGAGCTCCTTGGAGTGGAGGTTAAAATCGCTAATGATTGCGTCGGTGTGGAAGTTGAAAAGTTGGTTGCTGAGCTTCCAGAGGGAGGAGTTTTACTCCTTGAGAATGTCAGGTTCTACAAGGAGGAAGAGAAGAATGATCCCGAGTTTGCCAAGAAGCTTGCTTCACTTGCAGATGTCTATGTTAATGATGCTTTTGGCACTGCTCACAGGGCTCATGCATCCACAGAAGGAGTGGCGAAGTACTTGAAGCCTTCTGTTGCTGGATACCTTATGCAGAAGGAACTTGACTATCTTGTTGGTGCTGTGGCAAATCCCAAGAGACCATTTGCTGCTATTGTTGGTGGTTCGAAGGTGTCATCCAAGATTGGAGTCATAGAGTCCTTATTGGCGAAGGTTAACATTCTAGTGTTGGGTGGAGGAATGATCTTTACCTTTTATAAGGCTCAAGGGTATTCAGTTGGATCTTCCCTTGTTGAGGAAGACAAGCTAGATCTTGCAAAATCACTTATGGAGAAGGCAAAGGCCAAGGGAGTTTCTATTCTGCTCCCAACTGATGTGGTTATTGCTGATAAGTTTGCTGCTGATGCCAACAGCAAGGTGGTGCCAGCGTCTGCCATTCCAGATGGTTGGATGGGTTTGGATATCGGACCAGATTCCATCAAGACTTTCAATGAAGCTCTGGACACCACTAAGACTGTTATATGGAATGGACCTATGGGTGTGTTTGAGTTTGAAAAGTTTGCTGCTGGCACCGAGGTAAATAAAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002237 GO:0003674 GO:0003824 GO:0004618 GO:0004672 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0005911 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006464 GO:0006468 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009266 GO:0009314 GO:0009408 GO:0009416 GO:0009506 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009743 GO:0009746 GO:0009749 GO:0009987 GO:0010033 GO:0016020 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016774 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019538 GO:0019637 GO:0019693 GO:0019752 GO:0030054 GO:0031090 GO:0032787 GO:0034284 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0036211 GO:0042221 GO:0042866 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044267 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0048046 GO:0050896 GO:0051186 GO:0051188 GO:0051704 GO:0051707 GO:0055044 GO:0055086 GO:0071704 GO:0071944 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:0098588 GO:0098805 GO:0140096 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00010 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
PGK

Protein Analysis

281

Amino Acids

30.03

Weight (kDa)

5.88

Isoelectric Point (pI)

30.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGK PF00162 5 - 277 2.9e-117 Phosphoglycerate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 513
AatII GACGTC 1 cut(s) 22
AccB1I GGYRCC 2 cut(s) 676, 824
AclWI GGATC 2 cut(s) 206, 544
AcyI GRCGYC 1 cut(s) 19
AfaI GTAC 2 cut(s) 44, 317
AfiI CCNNNNNNNGG 3 cut(s) 88, 158, 550
AgsI TTSAA 5 cut(s) 52, 131, 322, 745, 809
AluBI AGCT 6 cut(s) 79, 148, 232, 562, 752, 843
AluI AGCT 6 cut(s) 79, 148, 232, 562, 752, 843
Alw21I GWGCWC 1 cut(s) 81
Alw26I GTCTC 1 cut(s) 388
AlwI GGATC 2 cut(s) 206, 544
AlwNI CAGNNNCTG 1 cut(s) 686
Ama87I CYCGRG 1 cut(s) 215
AoxI GGCC 1 cut(s) 597
ApeKI GCWGC 3 cut(s) 404, 653, 818
AspS9I GGNCC 2 cut(s) 722, 785
AsuII TTCGAA 1 cut(s) 422
AvaI CYCGRG 1 cut(s) 215
AvaII GGWCC 2 cut(s) 722, 785
BanI GGYRCC 2 cut(s) 676, 824
BanII GRGCYC 2 cut(s) 81, 289
BbsI GAAGAC 1 cut(s) 561
Bbv12I GWGCWC 1 cut(s) 81
BbvI GCAGC 3 cut(s) 391, 640, 805
BccI CCATC 3 cut(s) 692, 700, 740
BciVI GTATCC 1 cut(s) 332
BcoDI GTCTC 1 cut(s) 388
BfaI CTAG 2 cut(s) 479, 563
BfuI GTATCC 1 cut(s) 332
BglII AGATCT 1 cut(s) 565
BisI GCNGC 3 cut(s) 405, 654, 819
BlpI GCTNAGC 1 cut(s) 144
BlsI GCNGC 3 cut(s) 406, 655, 820
BmcAI AGTACT 1 cut(s) 317
Bme18I GGWCC 2 cut(s) 722, 785
BmeT110I CYCGRG 1 cut(s) 215
BmgT120I GGNCC 2 cut(s) 722, 785
BmiI GGNNCC 2 cut(s) 678, 826
BmsI GCATC 3 cut(s) 253, 302, 649
BpiI GAAGAC 1 cut(s) 561
BplI GAGNNNNNCTC 2 cut(s) 153, 185
Bpu1102I GCTNAGC 1 cut(s) 144
Bpu14I TTCGAA 1 cut(s) 422
BpuEI CTTGAG 2 cut(s) 194, 504
BsaHI GRCGYC 1 cut(s) 19
BsaI GGTCTC 1 cut(s) 388
BsaJI CCNNGG 3 cut(s) 82, 600, 828
BsaXI ACNNNNNCTCC 2 cut(s) 78, 108
Bsc4I CCNNNNNNNGG 3 cut(s) 88, 158, 550
BseDI CCNNGG 3 cut(s) 82, 600, 828
BseGI GGATG 3 cut(s) 293, 431, 711
BseLI CCNNNNNNNGG 3 cut(s) 88, 158, 550
BseMII CTCAG 2 cut(s) 66, 135
BseRI GAGGAG 1 cut(s) 174
BseXI GCAGC 3 cut(s) 391, 640, 805
BshFI GGCC 1 cut(s) 599
BshNI GGYRCC 2 cut(s) 676, 824
BsiHKAI GWGCWC 1 cut(s) 81
BsiHKCI CYCGRG 1 cut(s) 215
BslFI GGGAC 1 cut(s) 30
BslI CCNNNNNNNGG 3 cut(s) 88, 158, 550
BsmAI GTCTC 1 cut(s) 388
BsmFI GGGAC 1 cut(s) 30
BsnI GGCC 1 cut(s) 599
Bso31I GGTCTC 1 cut(s) 388
BsoBI CYCGRG 1 cut(s) 215
Bsp119I TTCGAA 1 cut(s) 422
Bsp1286I GDGCHC 2 cut(s) 81, 289
Bsp143I GATC 4 cut(s) 211, 498, 536, 565
Bsp1720I GCTNAGC 1 cut(s) 144
BspANI GGCC 1 cut(s) 599
BspCNI CTCAG 2 cut(s) 67, 136
BspLI GGNNCC 2 cut(s) 678, 826
BspPI GGATC 2 cut(s) 206, 544
BspT104I TTCGAA 1 cut(s) 422
BspT107I GGYRCC 2 cut(s) 676, 824
BspTNI GGTCTC 1 cut(s) 388
BssECI CCNNGG 3 cut(s) 82, 600, 828
BssMI GATC 4 cut(s) 211, 498, 536, 565
BssNI GRCGYC 1 cut(s) 19
BssT1I CCWWGG 2 cut(s) 82, 600
Bst4CI ACNGT 3 cut(s) 47, 72, 772
Bst6I CTCTTC 1 cut(s) 195
BstACI GRCGYC 1 cut(s) 19
BstBI TTCGAA 1 cut(s) 422
BstC8I GCNNGC 3 cut(s) 234, 681, 823
BstDEI CTNAG 3 cut(s) 75, 144, 765
BstENI CCTNNNNNAGG 1 cut(s) 548
BstF5I GGATG 3 cut(s) 293, 431, 711
BstKTI GATC 4 cut(s) 214, 501, 539, 568
BstMAI GTCTC 1 cut(s) 388
BstMBI GATC 4 cut(s) 211, 498, 536, 565
BstMWI GCNNNNNNNGC 4 cut(s) 61, 242, 284, 659
BstV1I GCAGC 3 cut(s) 391, 640, 805
BstV2I GAAGAC 1 cut(s) 561
BstX2I RGATCY 2 cut(s) 536, 565
BstXI CCANNNNNNTGG 1 cut(s) 702
BstYI RGATCY 2 cut(s) 536, 565
BsuI GTATCC 1 cut(s) 332
BsuRI GGCC 1 cut(s) 599
BtsCI GGATG 3 cut(s) 293, 431, 711
BtsI GCAGTG 1 cut(s) 273
BtsIMutI CAGTG 1 cut(s) 273
Cac8I GCNNGC 3 cut(s) 234, 681, 823
CaiI CAGNNNCTG 1 cut(s) 686
Cfr13I GGNCC 2 cut(s) 722, 785
CseI GACGC 2 cut(s) 103, 672
Csp6I GTAC 2 cut(s) 43, 316
CspCI CAANNNNNGTGG 2 cut(s) 612, 647
CviAII CATG 1 cut(s) 290
CviQI GTAC 2 cut(s) 43, 316
DdeI CTNAG 3 cut(s) 75, 144, 765
DpnI GATC 4 cut(s) 213, 500, 538, 567
DpnII GATC 4 cut(s) 211, 498, 536, 565
Eam1104I CTCTTC 1 cut(s) 195
EarI CTCTTC 1 cut(s) 195
Ecl136II GAGCTC 1 cut(s) 79
Eco130I CCWWGG 2 cut(s) 82, 600
Eco24I GRGCYC 2 cut(s) 81, 289
Eco31I GGTCTC 1 cut(s) 388
Eco32I GATATC 1 cut(s) 718
Eco47I GGWCC 2 cut(s) 722, 785
Eco53kI GAGCTC 1 cut(s) 79
Eco88I CYCGRG 1 cut(s) 215
EcoICRI GAGCTC 1 cut(s) 79
EcoNI CCTNNNNNAGG 1 cut(s) 548
EcoRV GATATC 1 cut(s) 718
EcoT14I CCWWGG 2 cut(s) 82, 600
EcoT22I ATGCAT 1 cut(s) 295
EcoT38I GRGCYC 2 cut(s) 81, 289
ErhI CCWWGG 2 cut(s) 82, 600
FaeI CATG 1 cut(s) 293
FaiI YATR 9 cut(s) 255, 291, 347, 449, 513, 584, 776, 778, 791
FaqI GGGAC 1 cut(s) 30
FatI CATG 1 cut(s) 289
Fnu4HI GCNGC 3 cut(s) 405, 654, 819
FokI GGATG 3 cut(s) 280, 418, 718
FriOI GRGCYC 2 cut(s) 81, 289
Fsp4HI GCNGC 3 cut(s) 405, 654, 819
FspBI CTAG 2 cut(s) 479, 563
GluI GCNGC 3 cut(s) 405, 654, 819
HaeIII GGCC 1 cut(s) 599
HgaI GACGC 2 cut(s) 103, 672
Hin1I GRCGYC 1 cut(s) 19
Hin1II CATG 1 cut(s) 293
HincII GTYRAC 1 cut(s) 472
HindII GTYRAC 1 cut(s) 472
HindIII AAGCTT 1 cut(s) 230
HinfI GANTC 3 cut(s) 444, 452, 728
HpaI GTTAAC 1 cut(s) 472
Hpy166II GTNNAC 2 cut(s) 11, 472
Hpy188I TCNGA 2 cut(s) 76, 722
Hpy188III TCNNGA 5 cut(s) 152, 215, 695, 736, 755
Hpy8I GTNNAC 2 cut(s) 11, 472
Hpy99I CGWCG 1 cut(s) 119
HpyAV CCTTC 6 cut(s) 296, 336, 346, 418, 460, 583
HpyCH4III ACNGT 3 cut(s) 47, 72, 772
HpyCH4IV ACGT 1 cut(s) 19
HpyCH4V TGCA 4 cut(s) 245, 293, 349, 572
HpyF10VI GCNNNNNNNGC 4 cut(s) 61, 242, 284, 659
HpyF3I CTNAG 3 cut(s) 75, 144, 765
HpySE526I ACGT 1 cut(s) 19
Hsp92I GRCGYC 1 cut(s) 19
Hsp92II CATG 1 cut(s) 293
KspAI GTTAAC 1 cut(s) 472
Kzo9I GATC 4 cut(s) 211, 498, 536, 565
LmnI GCTCC 2 cut(s) 84, 624
LpnPI CCDG 9 cut(s) 165, 169, 268, 321, 693, 708, 738, 740, 807
Lsp1109I GCAGC 3 cut(s) 391, 640, 805
LweI GCATC 3 cut(s) 253, 302, 649
MaeI CTAG 2 cut(s) 479, 563
MaeII ACGT 1 cut(s) 19
MaeIII GTNAC 1 cut(s) 31
MalI GATC 4 cut(s) 213, 500, 538, 567
MboI GATC 4 cut(s) 211, 498, 536, 565
MboII GAAGA 4 cut(s) 212, 217, 531, 566
MflI RGATCY 2 cut(s) 536, 565
MhlI GDGCHC 2 cut(s) 81, 289
MlyI GAGTC 2 cut(s) 453, 461
MmeI TCCRAC 2 cut(s) 514, 683
MnlI CCTC 8 cut(s) 66, 85, 148, 152, 190, 485, 544, 823
Mph1103I ATGCAT 1 cut(s) 295
MseI TTAA 3 cut(s) 96, 258, 471
MslI CAYNNNNRTG 1 cut(s) 479
MwoI GCNNNNNNNGC 4 cut(s) 61, 242, 284, 659
NdeII GATC 4 cut(s) 211, 498, 536, 565
NlaIII CATG 1 cut(s) 293
NlaIV GGNNCC 2 cut(s) 678, 826
NmuCI GTSAC 1 cut(s) 31
NsiI ATGCAT 1 cut(s) 295
NspV TTCGAA 1 cut(s) 422
PfeI GAWTC 1 cut(s) 728
PkrI GCNGC 3 cut(s) 406, 655, 820
PleI GAGTC 2 cut(s) 452, 460
PpsI GAGTC 2 cut(s) 452, 460
PsiI TTATAA 1 cut(s) 513
Psp124BI GAGCTC 1 cut(s) 81
PspN4I GGNNCC 2 cut(s) 678, 826
PspPI GGNCC 2 cut(s) 722, 785
PstNI CAGNNNCTG 1 cut(s) 686
PsuI RGATCY 2 cut(s) 536, 565
RsaI GTAC 2 cut(s) 44, 317
RsaNI GTAC 2 cut(s) 43, 316
RseI CAYNNNNRTG 1 cut(s) 479
SacI GAGCTC 1 cut(s) 81
SaqAI TTAA 3 cut(s) 96, 258, 471
SatI GCNGC 3 cut(s) 405, 654, 819
Sau3AI GATC 4 cut(s) 211, 498, 536, 565
Sau96I GGNCC 2 cut(s) 722, 785
ScaI AGTACT 1 cut(s) 317
SchI GAGTC 2 cut(s) 453, 461
SduI GDGCHC 2 cut(s) 81, 289
SfaNI GCATC 3 cut(s) 253, 302, 649
SfuI TTCGAA 1 cut(s) 422
SinI GGWCC 2 cut(s) 722, 785
SmiMI CAYNNNNRTG 1 cut(s) 479
SmlI CTYRAG 2 cut(s) 173, 519
SmoI CTYRAG 2 cut(s) 173, 519
SspMI CTAG 2 cut(s) 479, 563
SstI GAGCTC 1 cut(s) 81
StyI CCWWGG 2 cut(s) 82, 600
TaaI ACNGT 3 cut(s) 47, 72, 772
TaiI ACGT 1 cut(s) 22
TaqI TCGA 1 cut(s) 422
TatI WGTACW 2 cut(s) 42, 315
TfiI GAWTC 1 cut(s) 728
Tru1I TTAA 3 cut(s) 96, 258, 471
Tru9I TTAA 3 cut(s) 96, 258, 471
TscAI CASTG 1 cut(s) 280
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 3 cut(s) 404, 653, 818
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 1 cut(s) 762
TspRI CASTG 1 cut(s) 280
VpaK11BI GGWCC 2 cut(s) 722, 785
XagI CCTNNNNNAGG 1 cut(s) 548
XcmI CCANNNNNNNNNTGG 2 cut(s) 304, 670
XspI CTAG 2 cut(s) 479, 563
ZraI GACGTC 1 cut(s) 20
ZrmI AGTACT 1 cut(s) 317
Zsp2I ATGCAT 1 cut(s) 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.