Rorug05G0083900

Belongs to the phosphoglycerate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0008242
Physical Location & Seq
Forward (+)
7238733 .. 7241682
2950 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0083900.1

Sequence Viewer

Length: 2637 bp
ATGTTGATGCTCTGCACAGCCTCACCAAACCAGTCATGGGTCTCTCCTTCTTCTTCTTACCATGACATAAAGCCAAAGCCTTTGAAGCCCTGCTTCATCTTCTTCAAACCCAATTCAACCACCTCTCTGTTATCTCTGAATTTCTCTAATTTTCGCGCCAAATTTCGTGTCTCGGCGACCACCCTCGCCGGAACCACTGACCGGAAAACTGCGGACAAGAATGTGAAGATTGGCGAACTCTGTGAATTGGGTAATCTGAAAGAGGCGGTGGAAATGGTGTGCGGGTCTGAAAGCTCCGATCTTGAGTTGGTGACTTACTGCTCCGTGTTGGAGCTCTGTGCCGGAACTAAGTCTCTGAAAGATGGGAAGAGGGTTCACTCTGTTCTTTGTGACCATGGAGTAGATGTTGATGGGTATGTGGGTGCAAAACTTGTGTTCATGTATGTGCATTGTGGGGATTTAAGAGAGGCGAGGCGGGTTTTCGATGAATTATCGAATGCGAAAGTGTTTCTGTGGAACCTTTTAATCAATGAGTATGCGAAGATTCGTAATTTTAGAGAAGGTGTGTACTTATTTGGGAAGATGCAGGAGTTGGGTATTCATCCGAATTCGTATACGTTTTCTTGTATTTTGAAGTGTTTTGCTGCTCTTGGGTGTGTTAAGGAAGGTGAGTGGGTTCATGGGTATGTGTGTAAACTGGGTTTTAGCTCTGATAATACTGTGGTGAATTCTCTGATGGCTTTCTATTTCAAGAATAGAAGAGTTGAAAGTGCACGGAAGGTGTTTGATGAATTGCGTGACAGAGATGTGATATCGTGGAATTCGATGATTAGTGCGTATGTGTTGAATGGTGCTGCGGAGAAGGGAGTTGAGGTTTTCAGGCAGATGTTTTGTTCTGGGATTGATGTGGACTTGGCTACGATTATCAATGTTCTAATGGCGTGTTCGGACTGTGGAGATCTGGCATTGGGTAGGGTGGTTCATGCTTATGCGATAAAAGCTGGTTTCGACATGGATGTTATGTTGTGTAATAATGTTCTAGATATGTATTCAAAGTGTGGCGATTTGAGTGATGCAGTTCGAGTGTTTGAGAAGATGGGTCAAAGAAGTGTTGTGTCGTGGACTTCGATGATAGCAGGTTATGTTCGTGAAGGTCTTTCGGACAAGGCAATCGAGTTGTTTTGTGAAATGGAAGAGAATGGTGTTAGCCCAGATGGTTATACAATCACAAGCATACTTCATGCTTGTGCTTGTAGTGGCTCGTTGGAAAAAGGCAAGGATATACACAAGTATATTAGGGAACATGGCATGGACTCGAGTTTGTTTGTCTGTAATTCGCTTATGGATATGTATGCAAAATGTGGAAGCATGGAAGACGCTGACTCAGTTTTTTCTCATATGCTTAGAAAGGACATTGTGTCATGGAATACCATGATCGGAGGTTACTCCAAAAACTGTCTTCCAAATGAAGCTCTTGAACTCTTTTCTGATATGCAACAGAAATTTAAACCTGACAGCATGACAATTGCTAGTGTCCTTCCCGCTTGTGCAAGCCTAGCAGCTCTAGACAGAGGTCAAGAGATCCATGGTTATTTACTGAGAAATGGATACTTCTTGGATCGATATGTTGCCAATGCACTTGTTGACATGTATGTCAAGTGCGGGGTACTAGTTCTTGCTCGGGTACTGTTTGATATGATTCCGGTGAAGGATCTGATCTCTTGGACTGTGATAATTGCTGGTTATGGGATGCATGGATTTGGAAGGGAAGCTATTGCTGCGTTTAATGAGATGAAAAAGGCTGGAATTGAACCGGACAGCATTTCCTTCATCTCTATACTTTATGCTTGCAGTCATTCTGGATTAGTTCAGGAAGGATGGAGATTCTTTGATATTATGAGAAATGATTACAGAATCGAGCCCATGTTGGAGCATTATGCTTGTATGGTAGATCTTCTTGCTCGTACTGGGAATCTAACCAAGGCATATAAATTTATTAACATGATGCCAATTGAGCCAGATGCCACAGTCTGGGGTTCCTTGCTCTGTGGTTGTAGGATTCACCATGATGTGAAACTAGCAGAGAAAGTTGCAGAGCATGTCTTTGAACTAGAGCCAGAAAACACTGGCTATTATATACTTCTAGCAAATATATATGCAGAGGCAGAAAAGTGGGAAGAGGTGAAGAAGTTGAGGGAGAGGATTGGTCGGCGAGGCTTGAAAAAGAATCCGGGCTGTAGTTGGATAGAAATCAAGGGAAAAGTTTATATCTTTGTTGCGGGAGACACTTCACACCCAGAGGCCATGAAGATAGAGTCCCTAGTGAACAAGTTTAGATTGAGGATGAAGGAAGATGGCTATAATCCTAAAATGCAGTACGCTTTGATTAATGCAGATGAGGTGGAAAAAGAGGTGGCTCTCTGTGGGCATAGTGAGAAGTTAGCCATAGCATTTGGGATACTGAACTTGCCACCCGGAAAAACCATACGGGTGACCAAGAATCTACGAGTCTGCGGTGACTGCCATGAGATGGCCAAGTTCATATCCAAGACATCTAGGAGGGAAATTGTTTTGAGAGATTCTAATCGCTTTCATCATATGAAGGATGGAAATTGTTCTTGTAGGGGCTTCTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002237 GO:0003674 GO:0003824 GO:0004618 GO:0004672 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0005911 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006464 GO:0006468 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009266 GO:0009314 GO:0009408 GO:0009416 GO:0009506 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009743 GO:0009746 GO:0009749 GO:0009987 GO:0010033 GO:0016020 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016774 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019538 GO:0019637 GO:0019693 GO:0019752 GO:0030054 GO:0031090 GO:0032787 GO:0034284 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0036211 GO:0042221 GO:0042866 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044267 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0048046 GO:0050896 GO:0051186 GO:0051188 GO:0051704 GO:0051707 GO:0055044 GO:0055086 GO:0071704 GO:0071944 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:0098588 GO:0098805 GO:0140096 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00010 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
PGK

Protein Analysis

878

Amino Acids

98.61

Weight (kDa)

6.76

Isoelectric Point (pI)

32.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 169 - 215 2.1e-10 PPR repeat family
PPR_2 PF13041 268 - 308 5.4e-06 PPR repeat family
PPR PF01535 271 - 301 6.5e-06 PPR repeat
PPR_2 PF13041 370 - 417 9e-15 PPR repeat family
PPR PF01535 372 - 402 5.7e-10 PPR repeat
PPR_3 PF13812 393 - 452 5.7e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 470 - 517 6e-10 PPR repeat family
PPR PF01535 473 - 501 7.1e-08 PPR repeat
PPR_2 PF13041 571 - 618 2e-09 PPR repeat family
PPR PF01535 573 - 603 6.1e-06 PPR repeat
E_motif PF20431 688 - 750 1.8e-20 E motif
DYW_deaminase PF14432 786 - 878 2.1e-38 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1652
Acc36I ACCTGC 1 cut(s) 1127
AccB7I CCANNNNNTGG 2 cut(s) 2031, 2530
AccI GTMKAC 1 cut(s) 614
AccII CGCG 1 cut(s) 156
AciI CCGC 9 cut(s) 212, 266, 282, 475, 857, 1542, 1662, 2279, 2514
AclWI GGATC 3 cut(s) 1576, 1626, 1719
AcoI YGGCCR 1 cut(s) 2532
AcsI RAATTY 7 cut(s) 139, 161, 607, 727, 820, 1502, 1991
AfaI GTAC 5 cut(s) 569, 1668, 1686, 1966, 2378
AfiI CCNNNNNNNGG 4 cut(s) 37, 201, 2031, 2530
AflIII ACRYGT 1 cut(s) 1647
AhdI GACNNNNNGTC 1 cut(s) 1417
AhlI ACTAGT 1 cut(s) 1669
AluBI AGCT 7 cut(s) 294, 334, 708, 1001, 1472, 1562, 1772
AluI AGCT 7 cut(s) 294, 334, 708, 1001, 1472, 1562, 1772
Alw21I GWGCWC 2 cut(s) 336, 775
Alw26I GTCTC 4 cut(s) 46, 175, 357, 2277
Alw44I GTGCAC 1 cut(s) 771
AlwI GGATC 3 cut(s) 1576, 1626, 1719
Ama87I CYCGRG 2 cut(s) 1315, 1680
AoxI GGCC 2 cut(s) 2301, 2532
ApaLI GTGCAC 1 cut(s) 771
ApeKI GCWGC 4 cut(s) 644, 854, 1559, 1778
ApoI RAATTY 7 cut(s) 139, 161, 607, 727, 820, 1502, 1991
ArsI GACNNNNNNTTYG 2 cut(s) 153, 185
AseI ATTAAT 1 cut(s) 2388
Asp700I GAANNNNTTC 1 cut(s) 2614
AspLEI GCGC 1 cut(s) 158
AsuC2I CCSGG 2 cut(s) 2232, 2475
AsuHPI GGTGA 9 cut(s) 15, 322, 680, 736, 1717, 2054, 2194, 2503, 2528
AvaI CYCGRG 2 cut(s) 1315, 1680
BaeGI GKGCMC 1 cut(s) 775
BalI TGGCCA 1 cut(s) 2534
BanII GRGCYC 2 cut(s) 336, 1923
BbsI GAAGAC 2 cut(s) 1380, 1451
Bbv12I GWGCWC 2 cut(s) 336, 775
BbvI GCAGC 4 cut(s) 631, 841, 1571, 1765
BccI CCATC 9 cut(s) 356, 404, 730, 1090, 1208, 1872, 2348, 2524, 2600
BciVI GTATCC 2 cut(s) 1601, 2451
BcnI CCSGG 2 cut(s) 2232, 2475
BcoDI GTCTC 4 cut(s) 46, 175, 357, 2277
BcuI ACTAGT 1 cut(s) 1669
BfmI CTRYAG 1 cut(s) 2236
BfuAI ACCTGC 1 cut(s) 1127
BfuI GTATCC 2 cut(s) 1601, 2451
BglII AGATCT 2 cut(s) 958, 1951
BisI GCNGC 4 cut(s) 645, 855, 1560, 1779
BlsI GCNGC 4 cut(s) 646, 856, 1561, 1780
Bme1390I CCNGG 2 cut(s) 2232, 2475
BmeRI GACNNNNNGTC 1 cut(s) 1417
BmeT110I CYCGRG 2 cut(s) 1315, 1680
BmiI GGNNCC 3 cut(s) 193, 518, 2038
BmrFI CCNGG 2 cut(s) 2232, 2475
BmrI ACTGGG 2 cut(s) 707, 1977
BmsI GCATC 5 cut(s) 573, 1063, 1740, 1995, 2011
BmuI ACTGGG 2 cut(s) 707, 1977
BoxI GACNNNNGTC 1 cut(s) 1572
BpiI GAAGAC 2 cut(s) 1380, 1451
BpuEI CTTGAG 1 cut(s) 323
BpuMI CCSGG 2 cut(s) 2232, 2475
Bsa29I ATCGAT 1 cut(s) 1621
BsaBI GATNNNNATC 2 cut(s) 2249, 2583
BsaI GGTCTC 1 cut(s) 46
BsaJI CCNNGG 3 cut(s) 394, 1585, 1980
BsaWI WCCGGW 3 cut(s) 201, 1702, 1813
BsaXI ACNNNNNCTCC 4 cut(s) 390, 420, 858, 888
Bsc4I CCNNNNNNNGG 4 cut(s) 37, 201, 2031, 2530
Bse1I ACTGG 4 cut(s) 31, 702, 1972, 2131
Bse8I GATNNNNATC 2 cut(s) 2249, 2583
BseCI ATCGAT 1 cut(s) 1621
BseDI CCNNGG 3 cut(s) 394, 1585, 1980
BseGI GGATG 6 cut(s) 601, 1021, 1755, 1883, 2349, 2611
BseJI GATNNNNATC 2 cut(s) 2249, 2583
BseLI CCNNNNNNNGG 4 cut(s) 37, 201, 2031, 2530
BseMII CTCAG 2 cut(s) 1398, 1589
BseNI ACTGG 4 cut(s) 31, 702, 1972, 2131
BseSI GKGCMC 1 cut(s) 775
BseXI GCAGC 4 cut(s) 631, 841, 1571, 1765
Bsh1236I CGCG 1 cut(s) 156
BshFI GGCC 2 cut(s) 2303, 2534
BshVI ATCGAT 1 cut(s) 1621
BsiHKAI GWGCWC 2 cut(s) 336, 775
BsiHKCI CYCGRG 2 cut(s) 1315, 1680
BsiSI CCGG 7 cut(s) 189, 202, 342, 1703, 1814, 2231, 2475
BslFI GGGAC 1 cut(s) 2302
BslI CCNNNNNNNGG 4 cut(s) 37, 201, 2031, 2530
BsmAI GTCTC 4 cut(s) 46, 175, 357, 2277
BsmFI GGGAC 1 cut(s) 2302
BsmI GAATGC 1 cut(s) 502
BsnI GGCC 2 cut(s) 2303, 2534
Bso31I GGTCTC 1 cut(s) 46
BsoBI CYCGRG 2 cut(s) 1315, 1680
Bsp1286I GDGCHC 3 cut(s) 336, 775, 1923
Bsp143I GATC 8 cut(s) 298, 958, 1434, 1581, 1618, 1711, 1716, 1951
Bsp19I CCATGG 2 cut(s) 394, 1585
BspACI CCGC 9 cut(s) 212, 266, 282, 475, 857, 1542, 1662, 2279, 2514
BspANI GGCC 2 cut(s) 2303, 2534
BspCNI CTCAG 2 cut(s) 1397, 1590
BspDI ATCGAT 1 cut(s) 1621
BspFNI CGCG 1 cut(s) 156
BspLI GGNNCC 3 cut(s) 193, 518, 2038
BspMI ACCTGC 1 cut(s) 1127
BspPI GGATC 3 cut(s) 1576, 1626, 1719
BspTNI GGTCTC 1 cut(s) 46
BsrI ACTGG 4 cut(s) 31, 702, 1972, 2131
BssECI CCNNGG 3 cut(s) 394, 1585, 1980
BssMI GATC 8 cut(s) 298, 958, 1434, 1581, 1618, 1711, 1716, 1951
BssNAI GTATAC 1 cut(s) 615
BssT1I CCWWGG 3 cut(s) 394, 1585, 1980
Bst1107I GTATAC 1 cut(s) 615
Bst4CI ACNGT 6 cut(s) 721, 953, 1457, 1689, 1729, 2029
Bst6I CTCTTC 4 cut(s) 362, 754, 1188, 2172
BstC8I GCNNGC 2 cut(s) 1552, 1849
BstDEI CTNAG 4 cut(s) 348, 1384, 1403, 1598
BstDSI CCRYGG 2 cut(s) 394, 1585
BstEII GGTNACC 1 cut(s) 2491
BstF5I GGATG 6 cut(s) 601, 1021, 1755, 1883, 2349, 2611
BstFNI CGCG 1 cut(s) 156
BstHHI GCGC 1 cut(s) 158
BstKTI GATC 8 cut(s) 301, 961, 1437, 1584, 1621, 1714, 1719, 1954
BstMAI GTCTC 4 cut(s) 46, 175, 357, 2277
BstMBI GATC 8 cut(s) 298, 958, 1434, 1581, 1618, 1711, 1716, 1951
BstMWI GCNNNNNNNGC 6 cut(s) 85, 998, 1556, 1778, 2014, 2520
BstNSI RCATGY 2 cut(s) 1651, 2102
BstPAI GACNNNNGTC 1 cut(s) 1572
BstPI GGTNACC 1 cut(s) 2491
BstSCI CCNGG 2 cut(s) 2230, 2473
BstSFI CTRYAG 1 cut(s) 2236
BstSLI GKGCMC 1 cut(s) 775
BstUI CGCG 1 cut(s) 156
BstV1I GCAGC 4 cut(s) 631, 841, 1571, 1765
BstV2I GAAGAC 2 cut(s) 1380, 1451
BstX2I RGATCY 4 cut(s) 958, 1581, 1711, 1951
BstYI RGATCY 4 cut(s) 958, 1581, 1711, 1951
BstZ17I GTATAC 1 cut(s) 615
Bsu15I ATCGAT 1 cut(s) 1621
BsuI GTATCC 2 cut(s) 1601, 2451
BsuRI GGCC 2 cut(s) 2303, 2534
BsuTUI ATCGAT 1 cut(s) 1621
BtgI CCRYGG 2 cut(s) 394, 1585
BtsCI GGATG 6 cut(s) 601, 1021, 1755, 1883, 2349, 2611
BtsIMutI CAGTG 2 cut(s) 195, 2124
BveI ACCTGC 1 cut(s) 1127
Cac8I GCNNGC 2 cut(s) 1552, 1849
CfoI GCGC 1 cut(s) 158
ClaI ATCGAT 1 cut(s) 1621
CseI GACGC 1 cut(s) 1385
Csp6I GTAC 5 cut(s) 568, 1667, 1685, 1965, 2377
CviQI GTAC 5 cut(s) 568, 1667, 1685, 1965, 2377
DdeI CTNAG 4 cut(s) 348, 1384, 1403, 1598
DpnI GATC 8 cut(s) 300, 960, 1436, 1583, 1620, 1713, 1718, 1953
DpnII GATC 8 cut(s) 298, 958, 1434, 1581, 1618, 1711, 1716, 1951
DraI TTTAAA 1 cut(s) 1507
DrdI GACNNNNNNGTC 1 cut(s) 1652
DriI GACNNNNNGTC 1 cut(s) 1417
DseDI GACNNNNNNGTC 1 cut(s) 1652
EaeI YGGCCR 1 cut(s) 2532
Eam1104I CTCTTC 4 cut(s) 362, 754, 1188, 2172
Eam1105I GACNNNNNGTC 1 cut(s) 1417
EarI CTCTTC 4 cut(s) 362, 754, 1188, 2172
Ecl136II GAGCTC 1 cut(s) 334
Eco130I CCWWGG 3 cut(s) 394, 1585, 1980
Eco24I GRGCYC 2 cut(s) 336, 1923
Eco31I GGTCTC 1 cut(s) 46
Eco32I GATATC 1 cut(s) 813
Eco53kI GAGCTC 1 cut(s) 334
Eco88I CYCGRG 2 cut(s) 1315, 1680
Eco91I GGTNACC 1 cut(s) 2491
EcoICRI GAGCTC 1 cut(s) 334
EcoO65I GGTNACC 1 cut(s) 2491
EcoRI GAATTC 3 cut(s) 607, 727, 820
EcoRV GATATC 1 cut(s) 813
EcoT14I CCWWGG 3 cut(s) 394, 1585, 1980
EcoT22I ATGCAT 1 cut(s) 1755
EcoT38I GRGCYC 2 cut(s) 336, 1923
ErhI CCWWGG 3 cut(s) 394, 1585, 1980
FalI AAGNNNNNCTT 2 cut(s) 77, 109
FaqI GGGAC 1 cut(s) 2302
FauI CCCGC 5 cut(s) 275, 468, 1549, 1655, 2272
FauNDI CATATG 2 cut(s) 1398, 2598
FblI GTMKAC 1 cut(s) 614
Fnu4HI GCNGC 4 cut(s) 645, 855, 1560, 1779
FokI GGATG 6 cut(s) 588, 1028, 1762, 1890, 2356, 2618
FriOI GRGCYC 2 cut(s) 336, 1923
Fsp4HI GCNGC 4 cut(s) 645, 855, 1560, 1779
GlaI GCGC 1 cut(s) 157
GluI GCNGC 4 cut(s) 645, 855, 1560, 1779
HaeIII GGCC 2 cut(s) 2303, 2534
HapII CCGG 7 cut(s) 189, 202, 342, 1703, 1814, 2231, 2475
HgaI GACGC 1 cut(s) 1385
HhaI GCGC 1 cut(s) 158
Hin6I GCGC 1 cut(s) 156
HinP1I GCGC 1 cut(s) 156
HincII GTYRAC 1 cut(s) 1645
HindII GTYRAC 1 cut(s) 1645
HpaII CCGG 7 cut(s) 189, 202, 342, 1703, 1814, 2231, 2475
HphI GGTGA 9 cut(s) 15, 322, 680, 736, 1717, 2054, 2194, 2503, 2528
Hpy166II GTNNAC 9 cut(s) 376, 568, 615, 695, 773, 910, 1122, 1645, 2326
Hpy188III TCNNGA 9 cut(s) 302, 751, 1040, 1148, 1475, 1565, 1577, 1860, 1871
Hpy8I GTNNAC 9 cut(s) 376, 568, 615, 695, 773, 910, 1122, 1645, 2326
HpyCH4III ACNGT 6 cut(s) 721, 953, 1457, 1689, 1729, 2029
HpyCH4IV ACGT 1 cut(s) 617
HpyF10VI GCNNNNNNNGC 6 cut(s) 85, 998, 1556, 1778, 2014, 2520
HpyF3I CTNAG 4 cut(s) 348, 1384, 1403, 1598
HpySE526I ACGT 1 cut(s) 617
HspAI GCGC 1 cut(s) 156
Kzo9I GATC 8 cut(s) 298, 958, 1434, 1581, 1618, 1711, 1716, 1951
LmnI GCTCC 4 cut(s) 299, 326, 331, 1930
Lsp1109I GCAGC 4 cut(s) 631, 841, 1571, 1765
LweI GCATC 5 cut(s) 573, 1063, 1740, 1995, 2011
MaeII ACGT 1 cut(s) 617
MaeIII GTNAC 6 cut(s) 310, 389, 797, 1442, 2491, 2516
MalI GATC 8 cut(s) 300, 960, 1436, 1583, 1620, 1713, 1718, 1953
MboI GATC 8 cut(s) 298, 958, 1434, 1581, 1618, 1711, 1716, 1951
MfeI CAATTG 2 cut(s) 1524, 2010
MflI RGATCY 4 cut(s) 958, 1581, 1711, 1951
MhlI GDGCHC 3 cut(s) 336, 775, 1923
MlsI TGGCCA 1 cut(s) 2534
MluNI TGGCCA 1 cut(s) 2534
MlyI GAGTC 4 cut(s) 1307, 1376, 2324, 2517
MmeI TCCRAC 4 cut(s) 309, 1245, 1908, 2222
Mox20I TGGCCA 1 cut(s) 2534
Mph1103I ATGCAT 1 cut(s) 1755
MroXI GAANNNNTTC 1 cut(s) 2614
MscI TGGCCA 1 cut(s) 2534
MseI TTAA 7 cut(s) 461, 524, 660, 1506, 1785, 1998, 2388
MslI CAYNNNNRTG 6 cut(s) 443, 684, 987, 1245, 2067, 2489
Msp20I TGGCCA 1 cut(s) 2534
MspI CCGG 7 cut(s) 189, 202, 342, 1703, 1814, 2231, 2475
MspR9I CCNGG 2 cut(s) 2232, 2475
MunI CAATTG 2 cut(s) 1524, 2010
Mva1269I GAATGC 1 cut(s) 502
MvnI CGCG 1 cut(s) 156
MwoI GCNNNNNNNGC 6 cut(s) 85, 998, 1556, 1778, 2014, 2520
NciI CCSGG 2 cut(s) 2232, 2475
NcoI CCATGG 2 cut(s) 394, 1585
NdeI CATATG 2 cut(s) 1398, 2598
NdeII GATC 8 cut(s) 298, 958, 1434, 1581, 1618, 1711, 1716, 1951
NlaIV GGNNCC 3 cut(s) 193, 518, 2038
NmeAIII GCCGAG 1 cut(s) 152
NmuCI GTSAC 5 cut(s) 310, 389, 797, 2491, 2516
NsiI ATGCAT 1 cut(s) 1755
NspI RCATGY 2 cut(s) 1651, 2102
PaeR7I CTCGAG 1 cut(s) 1315
PciI ACATGT 1 cut(s) 1647
PcsI WCGNNNNNNNCGW 2 cut(s) 821, 1124
PctI GAATGC 1 cut(s) 502
PdmI GAANNNNTTC 1 cut(s) 2614
PfeI GAWTC 9 cut(s) 544, 1699, 1884, 1914, 1972, 2059, 2227, 2500, 2579
PflMI CCANNNNNTGG 2 cut(s) 2031, 2530
PkrI GCNGC 4 cut(s) 646, 856, 1561, 1780
PleI GAGTC 4 cut(s) 1307, 1376, 2323, 2516
PpsI GAGTC 4 cut(s) 1307, 1376, 2323, 2516
PscI ACATGT 1 cut(s) 1647
PshAI GACNNNNGTC 1 cut(s) 1572
PshBI ATTAAT 1 cut(s) 2388
Psp124BI GAGCTC 1 cut(s) 336
PspEI GGTNACC 1 cut(s) 2491
PspN4I GGNNCC 3 cut(s) 193, 518, 2038
PspXI VCTCGAGB 1 cut(s) 1315
PsuI RGATCY 4 cut(s) 958, 1581, 1711, 1951
RsaI GTAC 5 cut(s) 569, 1668, 1686, 1966, 2378
RsaNI GTAC 5 cut(s) 568, 1667, 1685, 1965, 2377
RseI CAYNNNNRTG 6 cut(s) 443, 684, 987, 1245, 2067, 2489
SacI GAGCTC 1 cut(s) 336
SaqAI TTAA 7 cut(s) 461, 524, 660, 1506, 1785, 1998, 2388
SatI GCNGC 4 cut(s) 645, 855, 1560, 1779
Sau3AI GATC 8 cut(s) 298, 958, 1434, 1581, 1618, 1711, 1716, 1951
SchI GAGTC 4 cut(s) 1307, 1376, 2324, 2517
ScrFI CCNGG 2 cut(s) 2232, 2475
SduI GDGCHC 3 cut(s) 336, 775, 1923
SfaNI GCATC 5 cut(s) 573, 1063, 1740, 1995, 2011
SfcI CTRYAG 1 cut(s) 2236
Sfr274I CTCGAG 1 cut(s) 1315
SlaI CTCGAG 1 cut(s) 1315
SmiMI CAYNNNNRTG 6 cut(s) 443, 684, 987, 1245, 2067, 2489
SmlI CTYRAG 2 cut(s) 302, 1315
SmoI CTYRAG 2 cut(s) 302, 1315
SpeI ACTAGT 1 cut(s) 1669
SsiI CCGC 9 cut(s) 212, 266, 282, 475, 857, 1542, 1662, 2279, 2514
SstI GAGCTC 1 cut(s) 336
StyD4I CCNGG 2 cut(s) 2230, 2473
StyI CCWWGG 3 cut(s) 394, 1585, 1980
TaaI ACNGT 6 cut(s) 721, 953, 1457, 1689, 1729, 2029
TaiI ACGT 1 cut(s) 620
TatI WGTACW 1 cut(s) 567
TfiI GAWTC 9 cut(s) 544, 1699, 1884, 1914, 1972, 2059, 2227, 2500, 2579
Tru1I TTAA 7 cut(s) 461, 524, 660, 1506, 1785, 1998, 2388
Tru9I TTAA 7 cut(s) 461, 524, 660, 1506, 1785, 1998, 2388
TscAI CASTG 2 cut(s) 202, 2131
TseFI GTSAC 5 cut(s) 310, 389, 797, 2491, 2516
TseI GCWGC 4 cut(s) 644, 854, 1559, 1778
Tsp45I GTSAC 5 cut(s) 310, 389, 797, 2491, 2516
TspGWI ACGGA 2 cut(s) 313, 790
TspRI CASTG 2 cut(s) 202, 2131
Van91I CCANNNNNTGG 2 cut(s) 2031, 2530
VneI GTGCAC 1 cut(s) 771
VspI ATTAAT 1 cut(s) 2388
XapI RAATTY 7 cut(s) 139, 161, 607, 727, 820, 1502, 1991
XbaI TCTAGA 2 cut(s) 1039, 1564
XceI RCATGY 2 cut(s) 1651, 2102
XcmI CCANNNNNNNNNTGG 1 cut(s) 33
XhoI CTCGAG 1 cut(s) 1315
XmiI GTMKAC 1 cut(s) 614
XmnI GAANNNNTTC 1 cut(s) 2614
Zsp2I ATGCAT 1 cut(s) 1755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.