pycom05g21160

Belongs to the phosphoglycerate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0008242
Physical Location & Seq
Forward (+)
23837849 .. 23838883
1035 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g21160.1

Sequence Viewer

Length: 681 bp
ATGCAGAAGGAACTTGACTACCTTGTCGGTGCCGTAGCAAATCCTAAGAGGCCATTTACTGCCATTGTTGGCGGTGCAAAGGTGTCATCCAAGATTGGAGTGATAGAATCCTTGTTGGAGAAGGTTAACATTCTCTTACTCGGTGGAGGAATGATCTTTACCTTCTACAAGGCCCAAGGGCATTCAGTTGGATCTTCCCTTGTGGAGGAAGACAAGCTTGTTCTTGCAAAGTCACTTCTTGAGAAGGCCAAATCTAAGGGGGTTTCTATTCTCCTCCCAACTGATGTGGTTATTGCTGACAAGTTTGCTGCTGATGCAAACAGCAAGGTTGTGCCAGCTTCTGCTATTCCAGATGGGTGGATGGGATTGGATATTGGACCAGACTCGATCAAAATTTTCAGTGAAGCTCTGGATACCACTCAAACTGTTATCTGGAACGGACCCATGGGTGTTTTTGAGTTTGAGAAGTTTGCTGCTGGGACTGAGGCAATAGCTAAGAAGCTTGCAGAGCTGAGCGGCAAGGGGGTGACAACAATCATCGGAGGTGGTGACTCAGTTGCCGCTGTTGAGAAGGCTGGGCTTGCTGAGAAGATGAGCCACATCTCAACTGGAGGCGGTGCAAGCTTAGAGCTCCTCGAAGGCAAAACACTCCCCGGAGTCCTTGCTCTTGACGATGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002237 GO:0003674 GO:0003824 GO:0004618 GO:0004672 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0005911 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006464 GO:0006468 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009266 GO:0009314 GO:0009408 GO:0009416 GO:0009506 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009743 GO:0009746 GO:0009749 GO:0009987 GO:0010033 GO:0016020 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016774 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019538 GO:0019637 GO:0019693 GO:0019752 GO:0030054 GO:0031090 GO:0032787 GO:0034284 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0036211 GO:0042221 GO:0042866 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044267 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0048046 GO:0050896 GO:0051186 GO:0051188 GO:0051704 GO:0051707 GO:0055044 GO:0055086 GO:0071704 GO:0071944 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:0098588 GO:0098805 GO:0140096 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00010 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
PGK

Protein Analysis

227

Amino Acids

23.3

Weight (kDa)

5.17

Isoelectric Point (pI)

30.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGK PF00162 1 - 215 2.3e-88 Phosphoglycerate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 23
AccB1I GGYRCC 1 cut(s) 29
AccBSI CCGCTC 1 cut(s) 516
AciI CCGC 4 cut(s) 72, 516, 561, 615
AclWI GGATC 1 cut(s) 199
AcsI RAATTY 1 cut(s) 393
AfiI CCNNNNNNNGG 1 cut(s) 205
AluBI AGCT 8 cut(s) 217, 338, 407, 494, 502, 511, 624, 631
AluI AGCT 8 cut(s) 217, 338, 407, 494, 502, 511, 624, 631
Alw21I GWGCWC 1 cut(s) 633
AlwI GGATC 1 cut(s) 199
AlwNI CAGNNNCTG 1 cut(s) 341
AoxI GGCC 3 cut(s) 50, 171, 246
ApeKI GCWGC 2 cut(s) 308, 473
ApoI RAATTY 1 cut(s) 393
AspS9I GGNCC 3 cut(s) 172, 377, 440
AsuC2I CCSGG 1 cut(s) 654
AsuHPI GGTGA 2 cut(s) 538, 560
AvaII GGWCC 2 cut(s) 377, 440
BanI GGYRCC 1 cut(s) 29
BanII GRGCYC 1 cut(s) 633
BbsI GAAGAC 1 cut(s) 216
Bbv12I GWGCWC 1 cut(s) 633
BbvI GCAGC 2 cut(s) 295, 460
BccI CCATC 2 cut(s) 347, 355
BceAI ACGGC 1 cut(s) 17
BciVI GTATCC 1 cut(s) 406
BcnI CCSGG 1 cut(s) 654
BfuI GTATCC 1 cut(s) 406
BisI GCNGC 4 cut(s) 309, 474, 517, 561
BlpI GCTNAGC 1 cut(s) 512
BlsI GCNGC 4 cut(s) 310, 475, 518, 562
Bme1390I CCNGG 1 cut(s) 654
Bme18I GGWCC 2 cut(s) 377, 440
BmgT120I GGNCC 3 cut(s) 172, 377, 440
BmiI GGNNCC 2 cut(s) 31, 442
BmrFI CCNGG 1 cut(s) 654
BmsI GCATC 2 cut(s) 304, 664
BpiI GAAGAC 1 cut(s) 216
BpmI CTGGAG 1 cut(s) 630
Bpu1102I GCTNAGC 1 cut(s) 512
BpuEI CTTGAG 1 cut(s) 260
BpuMI CCSGG 1 cut(s) 654
BsaJI CCNNGG 3 cut(s) 175, 444, 652
Bsc4I CCNNNNNNNGG 1 cut(s) 205
Bse1I ACTGG 1 cut(s) 613
BseDI CCNNGG 3 cut(s) 175, 444, 652
BseGI GGATG 2 cut(s) 86, 366
BseLI CCNNNNNNNGG 1 cut(s) 205
BseMII CTCAG 4 cut(s) 474, 503, 567, 576
BseNI ACTGG 1 cut(s) 613
BseRI GAGGAG 2 cut(s) 263, 623
BseXI GCAGC 2 cut(s) 295, 460
BseYI CCCAGC 2 cut(s) 476, 575
BshFI GGCC 3 cut(s) 52, 173, 248
BshNI GGYRCC 1 cut(s) 29
BsiHKAI GWGCWC 1 cut(s) 633
BsiSI CCGG 1 cut(s) 654
BslFI GGGAC 1 cut(s) 493
BslI CCNNNNNNNGG 1 cut(s) 205
BsmFI GGGAC 1 cut(s) 493
BsmI GAATGC 1 cut(s) 181
BsnI GGCC 3 cut(s) 52, 173, 248
Bsp1286I GDGCHC 1 cut(s) 633
Bsp143I GATC 3 cut(s) 153, 191, 387
Bsp1720I GCTNAGC 1 cut(s) 512
Bsp19I CCATGG 1 cut(s) 444
BspACI CCGC 4 cut(s) 72, 516, 561, 615
BspANI GGCC 3 cut(s) 52, 173, 248
BspCNI CTCAG 4 cut(s) 475, 504, 566, 577
BspLI GGNNCC 2 cut(s) 31, 442
BspPI GGATC 1 cut(s) 199
BspT107I GGYRCC 1 cut(s) 29
BsrBI CCGCTC 1 cut(s) 516
BsrI ACTGG 1 cut(s) 613
BssECI CCNNGG 3 cut(s) 175, 444, 652
BssMI GATC 3 cut(s) 153, 191, 387
BssT1I CCWWGG 2 cut(s) 175, 444
Bst4CI ACNGT 1 cut(s) 427
BstC8I GCNNGC 4 cut(s) 336, 504, 582, 622
BstDEI CTNAG 8 cut(s) 45, 255, 483, 495, 512, 553, 585, 625
BstDSI CCRYGG 1 cut(s) 444
BstENI CCTNNNNNAGG 1 cut(s) 203
BstF5I GGATG 2 cut(s) 86, 366
BstKTI GATC 3 cut(s) 156, 194, 390
BstMBI GATC 3 cut(s) 153, 191, 387
BstMWI GCNNNNNNNGC 4 cut(s) 314, 508, 581, 621
BstSCI CCNGG 1 cut(s) 652
BstV1I GCAGC 2 cut(s) 295, 460
BstV2I GAAGAC 1 cut(s) 216
BstX2I RGATCY 1 cut(s) 191
BstXI CCANNNNNNTGG 1 cut(s) 357
BstYI RGATCY 1 cut(s) 191
BsuI GTATCC 1 cut(s) 406
BsuRI GGCC 3 cut(s) 52, 173, 248
BtgI CCRYGG 1 cut(s) 444
BtsCI GGATG 2 cut(s) 86, 366
BtsIMutI CAGTG 1 cut(s) 406
Cac8I GCNNGC 4 cut(s) 336, 504, 582, 622
CaiI CAGNNNCTG 1 cut(s) 341
Cfr13I GGNCC 3 cut(s) 172, 377, 440
CspCI CAANNNNNGTGG 2 cut(s) 267, 302
CviAII CATG 1 cut(s) 445
DdeI CTNAG 8 cut(s) 45, 255, 483, 495, 512, 553, 585, 625
DpnI GATC 3 cut(s) 155, 193, 389
DpnII GATC 3 cut(s) 153, 191, 387
DrdI GACNNNNNNGTC 1 cut(s) 23
DseDI GACNNNNNNGTC 1 cut(s) 23
Ecl136II GAGCTC 1 cut(s) 631
Eco130I CCWWGG 2 cut(s) 175, 444
Eco24I GRGCYC 1 cut(s) 633
Eco47I GGWCC 2 cut(s) 377, 440
Eco53kI GAGCTC 1 cut(s) 631
EcoICRI GAGCTC 1 cut(s) 631
EcoNI CCTNNNNNAGG 1 cut(s) 203
EcoT14I CCWWGG 2 cut(s) 175, 444
EcoT38I GRGCYC 1 cut(s) 633
ErhI CCWWGG 2 cut(s) 175, 444
FaeI CATG 1 cut(s) 448
FaiI YATR 1 cut(s) 446
FalI AAGNNNNNCTT 2 cut(s) 201, 233
FaqI GGGAC 1 cut(s) 493
FatI CATG 1 cut(s) 444
Fnu4HI GCNGC 4 cut(s) 309, 474, 517, 561
FokI GGATG 2 cut(s) 73, 373
FriOI GRGCYC 1 cut(s) 633
Fsp4HI GCNGC 4 cut(s) 309, 474, 517, 561
GluI GCNGC 4 cut(s) 309, 474, 517, 561
GsaI CCCAGC 2 cut(s) 480, 579
GsuI CTGGAG 1 cut(s) 630
HaeIII GGCC 3 cut(s) 52, 173, 248
HapII CCGG 1 cut(s) 654
Hin1II CATG 1 cut(s) 448
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HindIII AAGCTT 3 cut(s) 215, 500, 622
HinfI GANTC 4 cut(s) 107, 383, 551, 657
HpaI GTTAAC 1 cut(s) 127
HpaII CCGG 1 cut(s) 654
HphI GGTGA 2 cut(s) 538, 560
Hpy166II GTNNAC 1 cut(s) 127
Hpy188I TCNGA 1 cut(s) 542
Hpy188III TCNNGA 5 cut(s) 239, 350, 410, 433, 668
Hpy8I GTNNAC 1 cut(s) 127
HpyAV CCTTC 5 cut(s) 115, 172, 238, 565, 632
HpyCH4III ACNGT 1 cut(s) 427
HpyCH4V TGCA 6 cut(s) 4, 77, 227, 317, 506, 620
HpyF10VI GCNNNNNNNGC 4 cut(s) 314, 508, 581, 621
HpyF3I CTNAG 8 cut(s) 45, 255, 483, 495, 512, 553, 585, 625
Hsp92II CATG 1 cut(s) 448
KspAI GTTAAC 1 cut(s) 127
Kzo9I GATC 3 cut(s) 153, 191, 387
LmnI GCTCC 1 cut(s) 636
LpnPI CCDG 9 cut(s) 348, 363, 393, 395, 418, 462, 561, 594, 667
Lsp1109I GCAGC 2 cut(s) 295, 460
LweI GCATC 2 cut(s) 304, 664
MaeIII GTNAC 3 cut(s) 231, 526, 548
MalI GATC 3 cut(s) 155, 193, 389
MbiI CCGCTC 1 cut(s) 516
MboI GATC 3 cut(s) 153, 191, 387
MboII GAAGA 3 cut(s) 186, 221, 601
MflI RGATCY 1 cut(s) 191
MhlI GDGCHC 1 cut(s) 633
MluCI AATT 1 cut(s) 393
MlyI GAGTC 3 cut(s) 377, 545, 666
MmeI TCCRAC 2 cut(s) 96, 169
MnlI CCTC 8 cut(s) 42, 140, 199, 284, 478, 536, 605, 644
MseI TTAA 2 cut(s) 126, 679
MspA1I CMGCKG 1 cut(s) 563
MspI CCGG 1 cut(s) 654
MspR9I CCNGG 1 cut(s) 654
Mva1269I GAATGC 1 cut(s) 181
MwoI GCNNNNNNNGC 4 cut(s) 314, 508, 581, 621
NciI CCSGG 1 cut(s) 654
NcoI CCATGG 1 cut(s) 444
NdeII GATC 3 cut(s) 153, 191, 387
NlaIII CATG 1 cut(s) 448
NlaIV GGNNCC 2 cut(s) 31, 442
NmuCI GTSAC 3 cut(s) 231, 526, 548
PctI GAATGC 1 cut(s) 181
PfeI GAWTC 1 cut(s) 107
PkrI GCNGC 4 cut(s) 310, 475, 518, 562
PleI GAGTC 3 cut(s) 377, 545, 665
PpsI GAGTC 3 cut(s) 377, 545, 665
Psp124BI GAGCTC 1 cut(s) 633
PspFI CCCAGC 2 cut(s) 476, 575
PspN4I GGNNCC 2 cut(s) 31, 442
PspPI GGNCC 3 cut(s) 172, 377, 440
PstNI CAGNNNCTG 1 cut(s) 341
PsuI RGATCY 1 cut(s) 191
SacI GAGCTC 1 cut(s) 633
SaqAI TTAA 2 cut(s) 126, 679
SatI GCNGC 4 cut(s) 309, 474, 517, 561
Sau3AI GATC 3 cut(s) 153, 191, 387
Sau96I GGNCC 3 cut(s) 172, 377, 440
SchI GAGTC 3 cut(s) 377, 545, 666
ScrFI CCNGG 1 cut(s) 654
SduI GDGCHC 1 cut(s) 633
SfaNI GCATC 2 cut(s) 304, 664
SinI GGWCC 2 cut(s) 377, 440
SmlI CTYRAG 1 cut(s) 239
SmoI CTYRAG 1 cut(s) 239
Sse9I AATT 1 cut(s) 393
SsiI CCGC 4 cut(s) 72, 516, 561, 615
SstI GAGCTC 1 cut(s) 633
StyD4I CCNGG 1 cut(s) 652
StyI CCWWGG 2 cut(s) 175, 444
TaaI ACNGT 1 cut(s) 427
TaqI TCGA 2 cut(s) 386, 636
TasI AATT 1 cut(s) 393
TauI GCSGC 2 cut(s) 519, 563
TfiI GAWTC 1 cut(s) 107
Tru1I TTAA 2 cut(s) 126, 679
Tru9I TTAA 2 cut(s) 126, 679
TscAI CASTG 1 cut(s) 406
TseFI GTSAC 3 cut(s) 231, 526, 548
TseI GCWGC 2 cut(s) 308, 473
Tsp45I GTSAC 3 cut(s) 231, 526, 548
TspGWI ACGGA 1 cut(s) 453
TspRI CASTG 1 cut(s) 406
VpaK11BI GGWCC 2 cut(s) 377, 440
XagI CCTNNNNNAGG 1 cut(s) 203
XapI RAATTY 1 cut(s) 393
XcmI CCANNNNNNNNNTGG 1 cut(s) 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.