Rh5BG173000

Belongs to the phosphoglycerate kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0008242
Physical Location & Seq
Forward (+)
18073819 .. 18075960
2142 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG173000.1

Sequence Viewer

Length: 1206 bp
ATGGCGACCAAGAGGAGTGTGAGTACTTTGAAGGAGGCTGATTTGAAGGGCAAGAGGGTTTTCGTTAGGGTTGATCTCAATGTTCCTTTGGATGACGACTGTAAGATCACCGATGACACTAGAGTCCGAGCTGCTGTCCCCACCATCAAGTACTTGCTCGGATATGGTGCCAAAGTCATCTTGGCTTCTCACTTGGGACGTCCAAAGGGTGTCACTCCCAAGTACAGTTTGAAGCCTCTTGTGCCAAGACTGTCTGAGCTCCTTGGAGTGGAGGTTAAAATCGCTAATGATTGCGTCGGTGCGGAAGTTGAAAAGTTGGTTGCTGAGCTTCCAGAGGGAGGAGTTTTGCTCCTTGAGAATGTCAGGTTCTACAAGGAGGAAGAGAAGAATGATCCCGAGTTCGCCAAGAAGCTTGCTTCACTTGCAGATGTCTATGTTAATGATGCTTTTGGCACTGCTCACAGGGCTCATGCATCCACCGAAGGAGTGGCGAAGTACTTGAAGCCTTCTGTTGCTGGATACCTTATGCAGAAGGAACTTGACTATCTTGTTGGTGCCGTGGCAAATCCCAAGAGACCATTTGCTGCTATTGTTGGTGGTTCAAAGGTGTCATCCAAGATTGGAGTGATAGAGTCATTATTGGCGAAGGTCAACATTCTAGTGTTGGGTGGAGGAATGATCTTTACCTTTTATAAGGCTCAAGGGTATTCAGTTGGATCTTCCCTTGTTGAGGAAGACAAGCTAGATCTTGCAAAATCACTTATGGAGAAGGCAAAGGCCAAGGGAGTTTCTATTCTGCTCCCAACTGATGTGGTTATTGCTGATAAGTTTGCTGCTGATGCCAACAGCAAGGTGGTGCCAGCGTCTGCCATTCCAGATGGTTGGATGGGTTTGGATATCGGACCAGATTCCATCAAGACTTTCAATGAAGCTCTGGACACCACTAAGACTGTTATATGGAATGGACCTATGGGTGTGTTCGAGTTTGAAAAGTTTGCTGCTGGCACCGAGGCGATTGCTAAGAAGCTTGCAGAGCTAAGTGGCAAGGGAGTTACAACTATCATTGGAGGTGGTGACTCTGTAGCTGCTGTGGAGAAGGTTGGCCTTGCTGACAAAATGAGCCACATTTCAACCGGAGGCGGTGCAAGCTTGGAGCTTCTTGAAGGGAAAACCCTCCCTGGAGTCCTTGCTCTTGACGACGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002237 GO:0003674 GO:0003824 GO:0004618 GO:0004672 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0005911 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006464 GO:0006468 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009266 GO:0009314 GO:0009408 GO:0009416 GO:0009506 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009743 GO:0009746 GO:0009749 GO:0009987 GO:0010033 GO:0016020 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016774 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019538 GO:0019637 GO:0019693 GO:0019752 GO:0030054 GO:0031090 GO:0032787 GO:0034284 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0036211 GO:0042221 GO:0042866 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044267 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0048046 GO:0050896 GO:0051186 GO:0051188 GO:0051704 GO:0051707 GO:0055044 GO:0055086 GO:0071704 GO:0071944 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:0098588 GO:0098805 GO:0140096 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00010 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
PGK

Protein Analysis

401

Amino Acids

42.36

Weight (kDa)

6.49

Isoelectric Point (pI)

24.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGK PF00162 10 - 390 7.2e-171 Phosphoglycerate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 693
AasI GACNNNNNNGTC 1 cut(s) 122
AatII GACGTC 1 cut(s) 202
AccB1I GGYRCC 4 cut(s) 167, 554, 856, 1004
AciI CCGC 2 cut(s) 302, 1140
AclWI GGATC 2 cut(s) 386, 724
AcyI GRCGYC 1 cut(s) 199
AfaI GTAC 4 cut(s) 25, 152, 224, 497
AfiI CCNNNNNNNGG 3 cut(s) 268, 338, 730
AjnI CCWGG 1 cut(s) 1177
Alw21I GWGCWC 1 cut(s) 261
Alw26I GTCTC 1 cut(s) 568
AlwI GGATC 2 cut(s) 386, 724
AlwNI CAGNNNCTG 1 cut(s) 866
Ama87I CYCGRG 1 cut(s) 395
AoxI GGCC 2 cut(s) 777, 1102
ApeKI GCWGC 5 cut(s) 131, 584, 833, 998, 1085
AspS9I GGNCC 2 cut(s) 902, 965
AsuHPI GGTGA 2 cut(s) 100, 1085
AvaI CYCGRG 1 cut(s) 395
AvaII GGWCC 2 cut(s) 902, 965
BanI GGYRCC 4 cut(s) 167, 554, 856, 1004
BanII GRGCYC 2 cut(s) 261, 469
BbsI GAAGAC 1 cut(s) 741
Bbv12I GWGCWC 1 cut(s) 261
BbvI GCAGC 5 cut(s) 118, 571, 820, 985, 1072
BccI CCATC 4 cut(s) 152, 872, 880, 920
BceAI ACGGC 1 cut(s) 542
BcgI CGANNNNNNTGC 2 cut(s) 998, 1032
BciT130I CCWGG 1 cut(s) 1179
BciVI GTATCC 1 cut(s) 512
BcoDI GTCTC 1 cut(s) 568
BfaI CTAG 3 cut(s) 120, 659, 743
BfmI CTRYAG 1 cut(s) 1080
BfuI GTATCC 1 cut(s) 512
BglII AGATCT 1 cut(s) 745
BisI GCNGC 5 cut(s) 132, 585, 834, 999, 1086
BlpI GCTNAGC 1 cut(s) 324
BlsI GCNGC 5 cut(s) 133, 586, 835, 1000, 1087
BmcAI AGTACT 3 cut(s) 25, 152, 497
Bme1390I CCNGG 1 cut(s) 1179
Bme18I GGWCC 2 cut(s) 902, 965
BmeT110I CYCGRG 1 cut(s) 395
BmgT120I GGNCC 2 cut(s) 902, 965
BmiI GGNNCC 4 cut(s) 169, 556, 858, 1006
BmrFI CCNGG 1 cut(s) 1179
BmsI GCATC 3 cut(s) 433, 482, 829
BpiI GAAGAC 1 cut(s) 741
BplI GAGNNNNNCTC 2 cut(s) 333, 365
BpmI CTGGAG 1 cut(s) 1200
Bpu1102I GCTNAGC 1 cut(s) 324
BpuEI CTTGAG 2 cut(s) 374, 684
BsaHI GRCGYC 1 cut(s) 199
BsaI GGTCTC 1 cut(s) 568
BsaJI CCNNGG 5 cut(s) 262, 558, 780, 1008, 1177
BsaWI WCCGGW 1 cut(s) 1133
BsaXI ACNNNNNCTCC 4 cut(s) 7, 37, 258, 288
Bsc4I CCNNNNNNNGG 3 cut(s) 268, 338, 730
BseBI CCWGG 1 cut(s) 1179
BseDI CCNNGG 5 cut(s) 262, 558, 780, 1008, 1177
BseGI GGATG 4 cut(s) 97, 473, 611, 891
BseLI CCNNNNNNNGG 3 cut(s) 268, 338, 730
BseMII CTCAG 2 cut(s) 246, 315
BseRI GAGGAG 2 cut(s) 28, 354
BseXI GCAGC 5 cut(s) 118, 571, 820, 985, 1072
BshFI GGCC 2 cut(s) 779, 1104
BshNI GGYRCC 4 cut(s) 167, 554, 856, 1004
BsiHKAI GWGCWC 1 cut(s) 261
BsiHKCI CYCGRG 1 cut(s) 395
BsiSI CCGG 1 cut(s) 1134
BslFI GGGAC 2 cut(s) 122, 210
BslI CCNNNNNNNGG 3 cut(s) 268, 338, 730
BsmAI GTCTC 1 cut(s) 568
BsmFI GGGAC 2 cut(s) 122, 210
BsnI GGCC 2 cut(s) 779, 1104
Bso31I GGTCTC 1 cut(s) 568
BsoBI CYCGRG 1 cut(s) 395
Bsp1286I GDGCHC 2 cut(s) 261, 469
Bsp143I GATC 6 cut(s) 73, 105, 391, 678, 716, 745
Bsp1720I GCTNAGC 1 cut(s) 324
BspACI CCGC 2 cut(s) 302, 1140
BspANI GGCC 2 cut(s) 779, 1104
BspCNI CTCAG 2 cut(s) 247, 316
BspLI GGNNCC 4 cut(s) 169, 556, 858, 1006
BspPI GGATC 2 cut(s) 386, 724
BspT107I GGYRCC 4 cut(s) 167, 554, 856, 1004
BspTNI GGTCTC 1 cut(s) 568
BssECI CCNNGG 5 cut(s) 262, 558, 780, 1008, 1177
BssMI GATC 6 cut(s) 73, 105, 391, 678, 716, 745
BssNI GRCGYC 1 cut(s) 199
BssT1I CCWWGG 2 cut(s) 262, 780
Bst2UI CCWGG 1 cut(s) 1179
Bst4CI ACNGT 4 cut(s) 101, 227, 252, 952
Bst6I CTCTTC 1 cut(s) 375
BstACI GRCGYC 1 cut(s) 199
BstC8I GCNNGC 5 cut(s) 414, 861, 1003, 1029, 1147
BstDEI CTNAG 5 cut(s) 255, 324, 945, 1020, 1037
BstDSI CCRYGG 1 cut(s) 558
BstENI CCTNNNNNAGG 1 cut(s) 728
BstF5I GGATG 4 cut(s) 97, 473, 611, 891
BstKTI GATC 6 cut(s) 76, 108, 394, 681, 719, 748
BstMAI GTCTC 1 cut(s) 568
BstMBI GATC 6 cut(s) 73, 105, 391, 678, 716, 745
BstMWI GCNNNNNNNGC 6 cut(s) 241, 422, 464, 839, 1033, 1146
BstNI CCWGG 1 cut(s) 1179
BstSCI CCNGG 1 cut(s) 1177
BstSFI CTRYAG 1 cut(s) 1080
BstV1I GCAGC 5 cut(s) 118, 571, 820, 985, 1072
BstV2I GAAGAC 1 cut(s) 741
BstX2I RGATCY 2 cut(s) 716, 745
BstXI CCANNNNNNTGG 1 cut(s) 882
BstYI RGATCY 2 cut(s) 716, 745
BsuI GTATCC 1 cut(s) 512
BsuRI GGCC 2 cut(s) 779, 1104
BtgI CCRYGG 1 cut(s) 558
BtsCI GGATG 4 cut(s) 97, 473, 611, 891
BtsI GCAGTG 1 cut(s) 453
BtsIMutI CAGTG 1 cut(s) 453
Cac8I GCNNGC 5 cut(s) 414, 861, 1003, 1029, 1147
CaiI CAGNNNCTG 1 cut(s) 866
Cfr13I GGNCC 2 cut(s) 902, 965
CseI GACGC 2 cut(s) 283, 852
Csp6I GTAC 4 cut(s) 24, 151, 223, 496
CspCI CAANNNNNGTGG 2 cut(s) 792, 827
CviAII CATG 1 cut(s) 470
CviQI GTAC 4 cut(s) 24, 151, 223, 496
DdeI CTNAG 5 cut(s) 255, 324, 945, 1020, 1037
DpnI GATC 6 cut(s) 75, 107, 393, 680, 718, 747
DpnII GATC 6 cut(s) 73, 105, 391, 678, 716, 745
DrdI GACNNNNNNGTC 1 cut(s) 122
DseDI GACNNNNNNGTC 1 cut(s) 122
Eam1104I CTCTTC 1 cut(s) 375
EarI CTCTTC 1 cut(s) 375
Ecl136II GAGCTC 1 cut(s) 259
Eco130I CCWWGG 2 cut(s) 262, 780
Eco24I GRGCYC 2 cut(s) 261, 469
Eco31I GGTCTC 1 cut(s) 568
Eco32I GATATC 1 cut(s) 898
Eco47I GGWCC 2 cut(s) 902, 965
Eco53kI GAGCTC 1 cut(s) 259
Eco88I CYCGRG 1 cut(s) 395
EcoICRI GAGCTC 1 cut(s) 259
EcoNI CCTNNNNNAGG 1 cut(s) 728
EcoRII CCWGG 1 cut(s) 1177
EcoRV GATATC 1 cut(s) 898
EcoT14I CCWWGG 2 cut(s) 262, 780
EcoT22I ATGCAT 1 cut(s) 475
EcoT38I GRGCYC 2 cut(s) 261, 469
ErhI CCWWGG 2 cut(s) 262, 780
FaeI CATG 1 cut(s) 473
FaiI YATR 9 cut(s) 165, 435, 471, 527, 693, 764, 956, 958, 971
FaqI GGGAC 2 cut(s) 122, 210
FatI CATG 1 cut(s) 469
Fnu4HI GCNGC 5 cut(s) 132, 585, 834, 999, 1086
FokI GGATG 4 cut(s) 104, 460, 598, 898
FriOI GRGCYC 2 cut(s) 261, 469
Fsp4HI GCNGC 5 cut(s) 132, 585, 834, 999, 1086
FspBI CTAG 3 cut(s) 120, 659, 743
GluI GCNGC 5 cut(s) 132, 585, 834, 999, 1086
GsuI CTGGAG 1 cut(s) 1200
HaeIII GGCC 2 cut(s) 779, 1104
HapII CCGG 1 cut(s) 1134
HgaI GACGC 2 cut(s) 283, 852
Hin1I GRCGYC 1 cut(s) 199
Hin1II CATG 1 cut(s) 473
HincII GTYRAC 1 cut(s) 652
HindII GTYRAC 1 cut(s) 652
HindIII AAGCTT 3 cut(s) 410, 1025, 1147
HinfI GANTC 5 cut(s) 123, 632, 908, 1076, 1182
HpaII CCGG 1 cut(s) 1134
HphI GGTGA 2 cut(s) 100, 1085
Hpy166II GTNNAC 1 cut(s) 652
Hpy188I TCNGA 4 cut(s) 128, 161, 256, 902
Hpy188III TCNNGA 7 cut(s) 332, 395, 875, 916, 935, 1160, 1193
Hpy8I GTNNAC 1 cut(s) 652
Hpy99I CGWCG 2 cut(s) 299, 1202
HpyAV CCTTC 9 cut(s) 25, 40, 476, 516, 526, 640, 763, 1090, 1157
HpyCH4III ACNGT 4 cut(s) 101, 227, 252, 952
HpyCH4IV ACGT 1 cut(s) 199
HpyCH4V TGCA 6 cut(s) 425, 473, 529, 752, 1031, 1145
HpyF10VI GCNNNNNNNGC 6 cut(s) 241, 422, 464, 839, 1033, 1146
HpyF3I CTNAG 5 cut(s) 255, 324, 945, 1020, 1037
HpySE526I ACGT 1 cut(s) 199
Hsp92I GRCGYC 1 cut(s) 199
Hsp92II CATG 1 cut(s) 473
Kzo9I GATC 6 cut(s) 73, 105, 391, 678, 716, 745
LmnI GCTCC 4 cut(s) 264, 354, 804, 1153
Lsp1109I GCAGC 5 cut(s) 118, 571, 820, 985, 1072
LweI GCATC 3 cut(s) 433, 482, 829
MaeI CTAG 3 cut(s) 120, 659, 743
MaeII ACGT 1 cut(s) 199
MaeIII GTNAC 3 cut(s) 211, 1051, 1073
MalI GATC 6 cut(s) 75, 107, 393, 680, 718, 747
MboI GATC 6 cut(s) 73, 105, 391, 678, 716, 745
MboII GAAGA 4 cut(s) 392, 397, 711, 746
MflI RGATCY 2 cut(s) 716, 745
MhlI GDGCHC 2 cut(s) 261, 469
MlyI GAGTC 4 cut(s) 132, 641, 1070, 1191
MmeI TCCRAC 2 cut(s) 694, 863
Mph1103I ATGCAT 1 cut(s) 475
MseI TTAA 3 cut(s) 276, 438, 1204
MslI CAYNNNNRTG 1 cut(s) 659
MspI CCGG 1 cut(s) 1134
MspR9I CCNGG 1 cut(s) 1179
MvaI CCWGG 1 cut(s) 1179
MwoI GCNNNNNNNGC 6 cut(s) 241, 422, 464, 839, 1033, 1146
NdeII GATC 6 cut(s) 73, 105, 391, 678, 716, 745
NlaIII CATG 1 cut(s) 473
NlaIV GGNNCC 4 cut(s) 169, 556, 858, 1006
NmuCI GTSAC 2 cut(s) 211, 1073
NsiI ATGCAT 1 cut(s) 475
PfeI GAWTC 1 cut(s) 908
PkrI GCNGC 5 cut(s) 133, 586, 835, 1000, 1087
PleI GAGTC 4 cut(s) 131, 640, 1070, 1190
PpsI GAGTC 4 cut(s) 131, 640, 1070, 1190
PsiI TTATAA 1 cut(s) 693
Psp124BI GAGCTC 1 cut(s) 261
Psp6I CCWGG 1 cut(s) 1177
PspGI CCWGG 1 cut(s) 1177
PspN4I GGNNCC 4 cut(s) 169, 556, 858, 1006
PspPI GGNCC 2 cut(s) 902, 965
PstNI CAGNNNCTG 1 cut(s) 866
PsuI RGATCY 2 cut(s) 716, 745
RsaI GTAC 4 cut(s) 25, 152, 224, 497
RsaNI GTAC 4 cut(s) 24, 151, 223, 496
RseI CAYNNNNRTG 1 cut(s) 659
SacI GAGCTC 1 cut(s) 261
SaqAI TTAA 3 cut(s) 276, 438, 1204
SatI GCNGC 5 cut(s) 132, 585, 834, 999, 1086
Sau3AI GATC 6 cut(s) 73, 105, 391, 678, 716, 745
Sau96I GGNCC 2 cut(s) 902, 965
ScaI AGTACT 3 cut(s) 25, 152, 497
SchI GAGTC 4 cut(s) 132, 641, 1070, 1191
ScrFI CCNGG 1 cut(s) 1179
SduI GDGCHC 2 cut(s) 261, 469
SfaNI GCATC 3 cut(s) 433, 482, 829
SfcI CTRYAG 1 cut(s) 1080
SinI GGWCC 2 cut(s) 902, 965
SmiMI CAYNNNNRTG 1 cut(s) 659
SmlI CTYRAG 2 cut(s) 353, 699
SmoI CTYRAG 2 cut(s) 353, 699
SsiI CCGC 2 cut(s) 302, 1140
SspMI CTAG 3 cut(s) 120, 659, 743
SstI GAGCTC 1 cut(s) 261
StyD4I CCNGG 1 cut(s) 1177
StyI CCWWGG 2 cut(s) 262, 780
TaaI ACNGT 4 cut(s) 101, 227, 252, 952
TaiI ACGT 1 cut(s) 202
TaqI TCGA 1 cut(s) 981
TatI WGTACW 4 cut(s) 23, 150, 222, 495
TfiI GAWTC 1 cut(s) 908
Tru1I TTAA 3 cut(s) 276, 438, 1204
Tru9I TTAA 3 cut(s) 276, 438, 1204
TscAI CASTG 1 cut(s) 460
TseFI GTSAC 2 cut(s) 211, 1073
TseI GCWGC 5 cut(s) 131, 584, 833, 998, 1085
Tsp45I GTSAC 2 cut(s) 211, 1073
TspDTI ATGAA 1 cut(s) 942
TspRI CASTG 1 cut(s) 460
VpaK11BI GGWCC 2 cut(s) 902, 965
XagI CCTNNNNNAGG 1 cut(s) 728
XcmI CCANNNNNNNNNTGG 3 cut(s) 178, 484, 850
XspI CTAG 3 cut(s) 120, 659, 743
ZraI GACGTC 1 cut(s) 200
ZrmI AGTACT 3 cut(s) 25, 152, 497
Zsp2I ATGCAT 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.