MD10G1210800.v1.1

Transglycosylase SLT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0012112
Physical Location & Seq
Reverse (-)
31014633 .. 31019150
4518 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1210800.v1.1.491

Sequence Viewer

Length: 1293 bp
ATGGCTGTCAGCTATAAATATTGGAATGACTGTGTTGATCCTCATGACCTGGAAGAAATGTGGAGTAGTCCTTATGTGGCTGCAGAATGGTTAGATGCTGGAGAGACCAAAGGCCAGAAAGTTCACATCTCGCGCGATCCCGATGGAAATGCGTATTTGACACAGATCGAGATGAAGGCTGTGGCTGAGATCATCATCTGCCGTCATTTTGGTTCACATATAGATCCTGATATGATTTGTGCTATTTCTGAACTTGCAAGTGATAGACAGCCCCTGGCTACGCAGTATGATAAAAAATCCAAGTCCACCTCTGTAGGGATCATGCAACTTTTTCCAAAGACCGTTGACTGGCTAGCTAGTGATTTGGGTTACCATGCATATGATATTCAAGGGAATCACGATCTTCTGTTCAAGCCTTTTGTAAATGTATATTTTGGTGCTGCGTATCTTAAATGGTTAGCAAAGTTTGATAACAAAGAAAGAGGTGAAGAATTCATAGTTAGGGCATATAAAGGTGGTACCAAGAAGGCAACTCACAAATCAACTTTGCAGTATTGGCAACGGTATCTTTCGGTCAAGGAAAGTCTTCCATCCAGAAAATGTTTTGAAGGTCATCGATCTCCAATCCATGGTTCTGCTTCTAATGCCCATCATGCTGCGCCGACTGCACCCTCACACAACACAGGTCATCATGTTTATTGGGATGCCAGAGCTTCCCCAGAAGACATGGCAGAAATGTGGAATCATCATGACGTCTCTCAAGAGTGGACTAGGACTGGAGAGAAAGAAGGAAAAGTACGATTCTCCATTGACGAAAAGAAAAGACCATATCTTTCTCGTGCAGAACTGAAGGCAGTTGCAGATATTATACTCACAAAGCACTTCAGTACAAAGCCAATGAAAGCTTCAGTTCTTTGTGCTCTTGCAGAGGTTGTTAGCTTGCGTTTCCTGAATGGTGTCGGACCACGTGCTGGAATAATGGGAATCGACTATTCTACGGCTTTATGGCTTTACAACGAGATGGGCTTTAGAGCATATAAACCTAATCAAGCCGATGATATTTGGAAGCCATTTTTCTCGATGTACTTTGGTGCAGCCTATTTAGTATGGTTAGTGGAATATGAAGGGAGGTTAAGAACTCCGGAGTTTGTTGTTCAGGCTTATATCTTGGGGCCAAAAAATGTGAATCTTCAGGATTCAGGTCCACAGTGGCTCAAGTTTGAACAAGCCTTGAGCCACTATGGAGACTCGAAGAAGTGTATTGAGGAGCACAGGAGCTGCTCCATCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SLT

Protein Analysis

431

Amino Acids

49.09

Weight (kDa)

7.03

Isoelectric Point (pI)

34.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SLT PF01464 72 - 185 7.2e-11 Transglycosylase SLT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012112)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16290 AT1G16290
fragaria_vesca FvH4_3g14650
malus_domestica MD05G1234300.v1.1 MD10G1210800.v1.1
prunus_persica Prupe.4G131800_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0024141
rosa_laevigata RLG00000032772
rosa_multiflora Rmu_sc0004033.1_g000015
rosa_roxburghii Rroxscaffold_1G00055100 Rroxscaffold_1G00055680
rosa_rugosa Rorug05G0081800 Rorug05G0081800 Rorug05G0081900
rosa_samantha Rh5AG172500 Rh5BG169100 Rh5CG184800 Rh5DG171300
rosa_wichuraiana Rw5G015560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 756
Acc65I GGTACC 1 cut(s) 518
AccB1I GGYRCC 1 cut(s) 518
AccB7I CCANNNNNTGG 2 cut(s) 629, 971
AccII CGCG 2 cut(s) 133, 135
AccIII TCCGGA 1 cut(s) 1141
AclWI GGATC 4 cut(s) 32, 131, 218, 326
AcsI RAATTY 1 cut(s) 491
AcuI CTGAAG 4 cut(s) 868, 869, 891, 1175
AcvI CACGTG 1 cut(s) 968
AcyI GRCGYC 1 cut(s) 753
AfaI GTAC 4 cut(s) 520, 798, 889, 1085
AfiI CCNNNNNNNGG 4 cut(s) 315, 348, 629, 971
AgsI TTSAA 4 cut(s) 389, 412, 608, 1223
AjnI CCWGG 2 cut(s) 48, 273
AjuI GAANNNNNNNTTGG 4 cut(s) 616, 648, 889, 921
AluBI AGCT 6 cut(s) 12, 356, 713, 905, 939, 1278
AluI AGCT 6 cut(s) 12, 356, 713, 905, 939, 1278
Alw21I GWGCWC 2 cut(s) 922, 1272
Alw26I GTCTC 3 cut(s) 98, 760, 1239
AlwI GGATC 4 cut(s) 32, 131, 218, 326
AlwNI CAGNNNCTG 2 cut(s) 274, 1278
Aor13HI TCCGGA 1 cut(s) 1141
AoxI GGCC 2 cut(s) 112, 1172
ApeKI GCWGC 5 cut(s) 80, 440, 656, 1094, 1278
ApoI RAATTY 1 cut(s) 491
Asp700I GAANNNNTTC 1 cut(s) 585
Asp718I GGTACC 1 cut(s) 518
AspLEI GCGC 2 cut(s) 135, 661
AspS9I GGNCC 3 cut(s) 962, 1172, 1202
AsuHPI GGTGA 1 cut(s) 497
AsuNHI GCTAGC 1 cut(s) 352
AvaII GGWCC 2 cut(s) 962, 1202
BanI GGYRCC 1 cut(s) 518
BarI GAAGNNNNNNTAC 2 cut(s) 780, 812
BauI CACGAG 1 cut(s) 837
BbrPI CACGTG 1 cut(s) 968
BbsI GAAGAC 2 cut(s) 578, 729
Bbv12I GWGCWC 2 cut(s) 922, 1272
BbvI GCAGC 5 cut(s) 67, 427, 643, 1106, 1265
BccI CCATC 5 cut(s) 137, 598, 657, 1015, 1292
BceAI ACGGC 2 cut(s) 186, 1014
BcgI CGANNNNNNTGC 2 cut(s) 131, 165
BciT130I CCWGG 2 cut(s) 50, 275
BcoDI GTCTC 3 cut(s) 98, 760, 1239
BfaI CTAG 3 cut(s) 353, 357, 771
BfmI CTRYAG 2 cut(s) 81, 312
BisI GCNGC 5 cut(s) 81, 441, 657, 1095, 1279
BlsI GCNGC 5 cut(s) 82, 442, 658, 1096, 1280
Bme1390I CCNGG 2 cut(s) 50, 275
Bme18I GGWCC 2 cut(s) 962, 1202
BmgT120I GGNCC 3 cut(s) 962, 1172, 1202
BmiI GGNNCC 2 cut(s) 520, 1173
BmrFI CCNGG 2 cut(s) 50, 275
BmsI GCATC 2 cut(s) 85, 694
BmtI GCTAGC 1 cut(s) 356
BpiI GAAGAC 2 cut(s) 578, 729
BpmI CTGGAG 2 cut(s) 120, 798
BpuEI CTTGAG 3 cut(s) 744, 1199, 1252
Bsa29I ATCGAT 1 cut(s) 616
BsaAI YACGTR 1 cut(s) 968
BsaBI GATNNNNATC 1 cut(s) 194
BsaHI GRCGYC 1 cut(s) 753
BsaI GGTCTC 1 cut(s) 98
BsaJI CCNNGG 2 cut(s) 273, 628
BsaWI WCCGGW 1 cut(s) 1141
Bsc4I CCNNNNNNNGG 4 cut(s) 315, 348, 629, 971
Bse1I ACTGG 2 cut(s) 353, 781
Bse8I GATNNNNATC 1 cut(s) 194
BseAI TCCGGA 1 cut(s) 1141
BseBI CCWGG 2 cut(s) 50, 275
BseCI ATCGAT 1 cut(s) 616
BseDI CCNNGG 2 cut(s) 273, 628
BseGI GGATG 2 cut(s) 590, 709
BseJI GATNNNNATC 1 cut(s) 194
BseLI CCNNNNNNNGG 4 cut(s) 315, 348, 629, 971
BseMII CTCAG 1 cut(s) 177
BseNI ACTGG 2 cut(s) 353, 781
BseRI GAGGAG 1 cut(s) 1280
BseXI GCAGC 5 cut(s) 67, 427, 643, 1106, 1265
BsgI GTGCAG 3 cut(s) 651, 861, 1113
Bsh1236I CGCG 2 cut(s) 133, 135
BshFI GGCC 2 cut(s) 114, 1174
BshNI GGYRCC 1 cut(s) 518
BshVI ATCGAT 1 cut(s) 616
BsiHKAI GWGCWC 2 cut(s) 922, 1272
BsiSI CCGG 1 cut(s) 1142
BslI CCNNNNNNNGG 4 cut(s) 315, 348, 629, 971
BsmAI GTCTC 3 cut(s) 98, 760, 1239
BsmBI CGTCTC 1 cut(s) 760
BsnI GGCC 2 cut(s) 114, 1174
Bso31I GGTCTC 1 cut(s) 98
Bsp1286I GDGCHC 2 cut(s) 922, 1272
Bsp13I TCCGGA 1 cut(s) 1141
Bsp143I GATC 8 cut(s) 37, 136, 165, 189, 223, 318, 400, 617
Bsp19I CCATGG 1 cut(s) 628
BspANI GGCC 2 cut(s) 114, 1174
BspCNI CTCAG 1 cut(s) 178
BspDI ATCGAT 1 cut(s) 616
BspEI TCCGGA 1 cut(s) 1141
BspFNI CGCG 2 cut(s) 133, 135
BspHI TCATGA 2 cut(s) 43, 748
BspLI GGNNCC 2 cut(s) 520, 1173
BspMAI CTGCAG 1 cut(s) 85
BspOI GCTAGC 1 cut(s) 356
BspPI GGATC 4 cut(s) 32, 131, 218, 326
BspT107I GGYRCC 1 cut(s) 518
BspTNI GGTCTC 1 cut(s) 98
BsrI ACTGG 2 cut(s) 353, 781
BssECI CCNNGG 2 cut(s) 273, 628
BssMI GATC 8 cut(s) 37, 136, 165, 189, 223, 318, 400, 617
BssNI GRCGYC 1 cut(s) 753
BssSI CACGAG 1 cut(s) 837
BssT1I CCWWGG 1 cut(s) 628
Bst2BI CACGAG 1 cut(s) 837
Bst2UI CCWGG 2 cut(s) 50, 275
Bst4CI ACNGT 4 cut(s) 32, 343, 564, 1209
BstACI GRCGYC 1 cut(s) 753
BstBAI YACGTR 1 cut(s) 968
BstC8I GCNNGC 2 cut(s) 354, 941
BstDEI CTNAG 1 cut(s) 186
BstDSI CCRYGG 1 cut(s) 628
BstEII GGTNACC 1 cut(s) 368
BstF5I GGATG 2 cut(s) 590, 709
BstFNI CGCG 2 cut(s) 133, 135
BstHHI GCGC 2 cut(s) 135, 661
BstKTI GATC 8 cut(s) 40, 139, 168, 192, 226, 321, 403, 620
BstMAI GTCTC 3 cut(s) 98, 760, 1239
BstMBI GATC 8 cut(s) 37, 136, 165, 189, 223, 318, 400, 617
BstMWI GCNNNNNNNGC 4 cut(s) 556, 644, 653, 665
BstNI CCWGG 2 cut(s) 50, 275
BstPI GGTNACC 1 cut(s) 368
BstSCI CCNGG 2 cut(s) 48, 273
BstSFI CTRYAG 2 cut(s) 81, 312
BstUI CGCG 2 cut(s) 133, 135
BstV1I GCAGC 5 cut(s) 67, 427, 643, 1106, 1265
BstV2I GAAGAC 2 cut(s) 578, 729
BstX2I RGATCY 1 cut(s) 223
BstYI RGATCY 1 cut(s) 223
Bsu15I ATCGAT 1 cut(s) 616
BsuRI GGCC 2 cut(s) 114, 1174
BsuTUI ATCGAT 1 cut(s) 616
BtgI CCRYGG 1 cut(s) 628
BtsCI GGATG 2 cut(s) 590, 709
BtsIMutI CAGTG 1 cut(s) 1214
Cac8I GCNNGC 2 cut(s) 354, 941
CaiI CAGNNNCTG 2 cut(s) 274, 1278
CciI TCATGA 2 cut(s) 43, 748
CfoI GCGC 2 cut(s) 135, 661
Cfr13I GGNCC 3 cut(s) 962, 1172, 1202
ClaI ATCGAT 1 cut(s) 616
Csp6I GTAC 4 cut(s) 519, 797, 888, 1084
CviAII CATG 9 cut(s) 44, 322, 374, 629, 653, 692, 727, 749, 1288
CviQI GTAC 4 cut(s) 519, 797, 888, 1084
DdeI CTNAG 1 cut(s) 186
DpnI GATC 8 cut(s) 39, 138, 167, 191, 225, 320, 402, 619
DpnII GATC 8 cut(s) 37, 136, 165, 189, 223, 318, 400, 617
Eco130I CCWWGG 1 cut(s) 628
Eco31I GGTCTC 1 cut(s) 98
Eco47I GGWCC 2 cut(s) 962, 1202
Eco57I CTGAAG 4 cut(s) 868, 869, 891, 1175
Eco72I CACGTG 1 cut(s) 968
Eco91I GGTNACC 1 cut(s) 368
EcoO65I GGTNACC 1 cut(s) 368
EcoRI GAATTC 1 cut(s) 491
EcoRII CCWGG 2 cut(s) 48, 273
EcoT14I CCWWGG 1 cut(s) 628
EcoT22I ATGCAT 1 cut(s) 379
ErhI CCWWGG 1 cut(s) 628
Esp3I CGTCTC 1 cut(s) 760
FaeI CATG 9 cut(s) 47, 325, 377, 632, 656, 695, 730, 752, 1291
FatI CATG 9 cut(s) 43, 321, 373, 628, 652, 691, 726, 748, 1287
FauNDI CATATG 1 cut(s) 379
Fnu4HI GCNGC 5 cut(s) 81, 441, 657, 1095, 1279
FokI GGATG 2 cut(s) 577, 716
Fsp4HI GCNGC 5 cut(s) 81, 441, 657, 1095, 1279
FspBI CTAG 3 cut(s) 353, 357, 771
GlaI GCGC 2 cut(s) 134, 660
GluI GCNGC 5 cut(s) 81, 441, 657, 1095, 1279
GsuI CTGGAG 2 cut(s) 120, 798
HaeIII GGCC 2 cut(s) 114, 1174
HapII CCGG 1 cut(s) 1142
HhaI GCGC 2 cut(s) 135, 661
Hin1I GRCGYC 1 cut(s) 753
Hin1II CATG 9 cut(s) 47, 325, 377, 632, 656, 695, 730, 752, 1291
Hin6I GCGC 2 cut(s) 133, 659
HinP1I GCGC 2 cut(s) 133, 659
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HindIII AAGCTT 1 cut(s) 903
HinfI GANTC 7 cut(s) 394, 742, 801, 984, 1186, 1196, 1247
HpaII CCGG 1 cut(s) 1142
HphI GGTGA 1 cut(s) 497
Hpy166II GTNNAC 6 cut(s) 124, 215, 306, 346, 768, 1205
Hpy188I TCNGA 2 cut(s) 250, 962
Hpy8I GTNNAC 6 cut(s) 124, 215, 306, 346, 768, 1205
HpyAV CCTTC 6 cut(s) 169, 520, 602, 782, 844, 1118
HpyCH4III ACNGT 4 cut(s) 32, 343, 564, 1209
HpyCH4IV ACGT 2 cut(s) 753, 967
HpyF10VI GCNNNNNNNGC 4 cut(s) 556, 644, 653, 665
HpyF3I CTNAG 1 cut(s) 186
HpySE526I ACGT 2 cut(s) 753, 967
Hsp92I GRCGYC 1 cut(s) 753
Hsp92II CATG 9 cut(s) 47, 325, 377, 632, 656, 695, 730, 752, 1291
HspAI GCGC 2 cut(s) 133, 659
Kpn2I TCCGGA 1 cut(s) 1141
KpnI GGTACC 1 cut(s) 522
Kzo9I GATC 8 cut(s) 37, 136, 165, 189, 223, 318, 400, 617
LmnI GCTCC 3 cut(s) 1267, 1275, 1286
Lsp1109I GCAGC 5 cut(s) 67, 427, 643, 1106, 1265
LweI GCATC 2 cut(s) 85, 694
MaeI CTAG 3 cut(s) 353, 357, 771
MaeII ACGT 2 cut(s) 753, 967
MaeIII GTNAC 1 cut(s) 368
MalI GATC 8 cut(s) 39, 138, 167, 191, 225, 320, 402, 619
MboI GATC 8 cut(s) 37, 136, 165, 189, 223, 318, 400, 617
MboII GAAGA 7 cut(s) 65, 395, 500, 578, 734, 1181, 1264
MflI RGATCY 1 cut(s) 223
MhlI GDGCHC 2 cut(s) 922, 1272
MluCI AATT 1 cut(s) 491
MlyI GAGTC 1 cut(s) 1241
MmeI TCCRAC 1 cut(s) 940
MnlI CCTC 7 cut(s) 51, 319, 476, 682, 922, 1122, 1258
Mph1103I ATGCAT 1 cut(s) 379
MroI TCCGGA 1 cut(s) 1141
MroXI GAANNNNTTC 1 cut(s) 585
MseI TTAA 2 cut(s) 450, 1133
MslI CAYNNNNRTG 1 cut(s) 378
MspI CCGG 1 cut(s) 1142
MspR9I CCNGG 2 cut(s) 50, 275
MvaI CCWGG 2 cut(s) 50, 275
MvnI CGCG 2 cut(s) 133, 135
MwoI GCNNNNNNNGC 4 cut(s) 556, 644, 653, 665
NcoI CCATGG 1 cut(s) 628
NdeI CATATG 1 cut(s) 379
NdeII GATC 8 cut(s) 37, 136, 165, 189, 223, 318, 400, 617
NheI GCTAGC 1 cut(s) 352
NlaIII CATG 9 cut(s) 47, 325, 377, 632, 656, 695, 730, 752, 1291
NlaIV GGNNCC 2 cut(s) 520, 1173
NsiI ATGCAT 1 cut(s) 379
PagI TCATGA 2 cut(s) 43, 748
PdmI GAANNNNTTC 1 cut(s) 585
PfeI GAWTC 6 cut(s) 394, 742, 801, 984, 1186, 1196
PflMI CCANNNNNTGG 2 cut(s) 629, 971
PkrI GCNGC 5 cut(s) 82, 442, 658, 1096, 1280
PleI GAGTC 1 cut(s) 1241
PmaCI CACGTG 1 cut(s) 968
PmlI CACGTG 1 cut(s) 968
PpsI GAGTC 1 cut(s) 1241
Ppu21I YACGTR 1 cut(s) 968
Psp6I CCWGG 2 cut(s) 48, 273
PspCI CACGTG 1 cut(s) 968
PspEI GGTNACC 1 cut(s) 368
PspGI CCWGG 2 cut(s) 48, 273
PspN4I GGNNCC 2 cut(s) 520, 1173
PspPI GGNCC 3 cut(s) 962, 1172, 1202
PstI CTGCAG 1 cut(s) 85
PstNI CAGNNNCTG 2 cut(s) 274, 1278
PsuI RGATCY 1 cut(s) 223
RsaI GTAC 4 cut(s) 520, 798, 889, 1085
RsaNI GTAC 4 cut(s) 519, 797, 888, 1084
RseI CAYNNNNRTG 1 cut(s) 378
SaqAI TTAA 2 cut(s) 450, 1133
SatI GCNGC 5 cut(s) 81, 441, 657, 1095, 1279
Sau3AI GATC 8 cut(s) 37, 136, 165, 189, 223, 318, 400, 617
Sau96I GGNCC 3 cut(s) 962, 1172, 1202
SchI GAGTC 1 cut(s) 1241
ScrFI CCNGG 2 cut(s) 50, 275
SduI GDGCHC 2 cut(s) 922, 1272
SfaNI GCATC 2 cut(s) 85, 694
SfcI CTRYAG 2 cut(s) 81, 312
SinI GGWCC 2 cut(s) 962, 1202
SmiMI CAYNNNNRTG 1 cut(s) 378
SmlI CTYRAG 3 cut(s) 759, 1214, 1231
SmoI CTYRAG 3 cut(s) 759, 1214, 1231
Sse9I AATT 1 cut(s) 491
SspI AATATT 1 cut(s) 20
SspMI CTAG 3 cut(s) 353, 357, 771
StyD4I CCNGG 2 cut(s) 48, 273
StyI CCWWGG 1 cut(s) 628
TaaI ACNGT 4 cut(s) 32, 343, 564, 1209
TaiI ACGT 2 cut(s) 756, 970
TaqI TCGA 5 cut(s) 168, 616, 987, 1079, 1250
TaqII GACCGA 1 cut(s) 562
TasI AATT 1 cut(s) 491
TatI WGTACW 2 cut(s) 887, 1083
TfiI GAWTC 6 cut(s) 394, 742, 801, 984, 1186, 1196
Tru1I TTAA 2 cut(s) 450, 1133
Tru9I TTAA 2 cut(s) 450, 1133
TscAI CASTG 1 cut(s) 1214
TseI GCWGC 5 cut(s) 80, 440, 656, 1094, 1278
TspDTI ATGAA 4 cut(s) 188, 484, 914, 1137
TspRI CASTG 1 cut(s) 1214
Van91I CCANNNNNTGG 2 cut(s) 629, 971
VpaK11BI GGWCC 2 cut(s) 962, 1202
XapI RAATTY 1 cut(s) 491
XmnI GAANNNNTTC 1 cut(s) 585
XspI CTAG 3 cut(s) 353, 357, 771
ZraI GACGTC 1 cut(s) 754
Zsp2I ATGCAT 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.