MD10G1211200.v1.1

Programmed cell death protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0006175
Physical Location & Seq
Reverse (-)
31044973 .. 31047967
2995 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1211200.v1.1.491

Sequence Viewer

Length: 1242 bp
ATGGCAGGCAGTGTGAAAGGTGACGGTACGGAGGAGTTTAAAGGCCTTAGAATCACATCACTGGACGATGACAGCGACGAAGAAGAAGAACAGAAAGAGCCAGATGTTGCTATGGATGACGATGACGAAGAAGAGGAAGACGAAGAAGCAAGAAATTCTGTGATTTTAGGTTTTGTGAAAAAGCCTAAGAAATCCCGCTCTCTTCTTCGCCATTTCTTTCCCTCCAAGGCTGGAGGCGTTCCCGCTTGGCTGGATCCAGAAAACTTGCCGTCCGGAAGATCTTGCTGCTGCGACATATGCGGTGAACCTTTGCAATTTGTGCTTCAGGTATATGCGCCAGAGGAGAAGGAATCTGCATTTCACCGAACATTGTTTGTGTTTATGTGTACGTCAATGGCGTGTCTTCGTCGTGATCAACATGAACAATGGAAATGCCAGCGGGACGAGCCCTCTAGAAGCGTGAAGGTTTTCCGCTGCCAATTACCTCGCGATAATCCTTTCTACTCAAGTGAGCCTCCGAAGAAGGATAGTACGGAGGATCCTCTTATGGCTGGAGCTGCACTATGTAATTGGTGTGGTACCTGGAAGGGGAGTAAACGCTGTACTGGTTGCAGAAAAACACTTTATTGCTCAGAGAAACATTGGGTCAAGCATTCTCGGTCTGGTCATGAACATGATTGTCAACGATTGAGGATTCAGCTGGCCGACAGTAGCTCTGCTAACAGTGAAACCACTTCTGCTGTAGTTCTAAAAGTTGCAAGTAATAATCTATGGCCAGAGTACAAAATTATACAGGAGCCTGAAAGTTCATATTACACGGAGATGCCTGATGAGAGTGTGGGCACTAATTCCTTGGTCTCTAGTAACCGAATGGATGATGGGCTAATGTCGATAGCAGACAATTTTACGGGTGATGGTGATAGAAAGAGCTGGGCTTCATTCCACGTGCGCATAGCCCTGGCCCCTGAACAAATACTAAGGTATTGTAGGAGTTCCGGTGCTAAACCCTTATGGCCTGTGTCAAGTGGCCGGCCATCCACGGCTGATATTCCTAAATGTAGTTATTGTAGTGGCCCAATGTGTTTTGAATTTCAGATTATGCCTCAGTTGCTATTTTACTTCAACGTGAAGAACGACGTGGACTCTCTTGATTGGGCTACAATCGCCGTGTATACATGTGAAGCCTCTTGTGATGCAAGTGTGGTTTACAAAGAAGAATTCGCTTGGGTTCAACTTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PDCD2_C zf-MYND

Protein Analysis

414

Amino Acids

46.6

Weight (kDa)

5.0

Isoelectric Point (pI)

58.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PDCD2_C PF04194 309 - 411 2.3e-32 Programmed cell death protein 2, C-terminal putative domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 950
Acc65I GGTACC 1 cut(s) 578
AccB1I GGYRCC 1 cut(s) 578
AccBSI CCGCTC 1 cut(s) 198
AccI GTMKAC 1 cut(s) 1172
AccII CGCG 1 cut(s) 489
AccIII TCCGGA 1 cut(s) 272
AciI CCGC 5 cut(s) 196, 243, 300, 439, 472
AclWI GGATC 4 cut(s) 248, 261, 533, 546
AcoI YGGCCR 4 cut(s) 702, 773, 1027, 1031
AcsI RAATTY 3 cut(s) 154, 1088, 1217
AcuI CTGAAG 1 cut(s) 308
AcvI CACGTG 1 cut(s) 946
AfaI GTAC 6 cut(s) 28, 388, 532, 580, 604, 782
AfiI CCNNNNNNNGG 1 cut(s) 588
AflIII ACRYGT 1 cut(s) 1175
AgsI TTSAA 3 cut(s) 1088, 1123, 1232
AjiI CACGTC 1 cut(s) 1138
AjnI CCWGG 2 cut(s) 581, 957
AluBI AGCT 4 cut(s) 557, 700, 714, 930
AluI AGCT 4 cut(s) 557, 700, 714, 930
Alw26I GTCTC 1 cut(s) 862
AlwI GGATC 4 cut(s) 248, 261, 533, 546
Aor13HI TCCGGA 1 cut(s) 272
AoxI GGCC 8 cut(s) 43, 702, 773, 960, 1013, 1027, 1031, 1072
ApeKI GCWGC 4 cut(s) 285, 288, 474, 557
ApoI RAATTY 3 cut(s) 154, 1088, 1217
Asp700I GAANNNNTTC 1 cut(s) 467
Asp718I GGTACC 1 cut(s) 578
AspLEI GCGC 2 cut(s) 337, 951
AspS9I GGNCC 2 cut(s) 961, 1073
AsuHPI GGTGA 5 cut(s) 32, 314, 353, 923, 929
BaeGI GKGCMC 1 cut(s) 845
BalI TGGCCA 1 cut(s) 775
BamHI GGATCC 2 cut(s) 253, 538
BanI GGYRCC 1 cut(s) 578
BanII GRGCYC 1 cut(s) 450
BbrPI CACGTG 1 cut(s) 946
BbsI GAAGAC 2 cut(s) 144, 395
BbvI GCAGC 4 cut(s) 272, 275, 461, 544
BccI CCATC 3 cut(s) 872, 908, 1042
BceAI ACGGC 3 cut(s) 253, 1056, 1151
BciT130I CCWGG 2 cut(s) 583, 959
BclI TGATCA 1 cut(s) 412
BcoDI GTCTC 1 cut(s) 862
BfaI CTAG 2 cut(s) 453, 861
BfmI CTRYAG 1 cut(s) 741
BglII AGATCT 1 cut(s) 278
BisI GCNGC 4 cut(s) 286, 289, 475, 558
BlsI GCNGC 4 cut(s) 287, 290, 476, 559
Bme1390I CCNGG 2 cut(s) 583, 959
BmgBI CACGTC 1 cut(s) 1138
BmgT120I GGNCC 2 cut(s) 961, 1073
BmiI GGNNCC 5 cut(s) 255, 540, 580, 798, 963
BmrFI CCNGG 2 cut(s) 583, 959
BmsI GCATC 2 cut(s) 813, 1183
BpiI GAAGAC 2 cut(s) 144, 395
BpmI CTGGAG 2 cut(s) 252, 573
BpuEI CTTGAG 1 cut(s) 490
BsaAI YACGTR 1 cut(s) 946
BsaI GGTCTC 1 cut(s) 862
BsaJI CCNNGG 4 cut(s) 225, 852, 957, 1038
BsaWI WCCGGW 2 cut(s) 272, 995
BsaXI ACNNNNNCTCC 2 cut(s) 982, 1012
Bsc4I CCNNNNNNNGG 1 cut(s) 588
Bse118I RCCGGY 1 cut(s) 1029
Bse1I ACTGG 2 cut(s) 66, 610
BseAI TCCGGA 1 cut(s) 272
BseBI CCWGG 2 cut(s) 583, 959
BseDI CCNNGG 4 cut(s) 225, 852, 957, 1038
BseGI GGATG 3 cut(s) 121, 880, 1034
BseLI CCNNNNNNNGG 1 cut(s) 588
BseMII CTCAG 2 cut(s) 645, 1118
BseNI ACTGG 2 cut(s) 66, 610
BseRI GAGGAG 2 cut(s) 47, 356
BseSI GKGCMC 1 cut(s) 845
BseXI GCAGC 4 cut(s) 272, 275, 461, 544
BseYI CCCAGC 1 cut(s) 930
BsgI GTGCAG 1 cut(s) 543
Bsh1236I CGCG 1 cut(s) 489
BshFI GGCC 8 cut(s) 45, 704, 775, 962, 1015, 1029, 1033, 1074
BshNI GGYRCC 1 cut(s) 578
BsiSI CCGG 3 cut(s) 273, 996, 1030
BslFI GGGAC 1 cut(s) 455
BslI CCNNNNNNNGG 1 cut(s) 588
BsmAI GTCTC 1 cut(s) 862
BsmFI GGGAC 1 cut(s) 455
BsmI GAATGC 1 cut(s) 652
BsnI GGCC 8 cut(s) 45, 704, 775, 962, 1015, 1029, 1033, 1074
Bso31I GGTCTC 1 cut(s) 862
Bsp1286I GDGCHC 2 cut(s) 450, 845
Bsp13I TCCGGA 1 cut(s) 272
Bsp143I GATC 4 cut(s) 253, 278, 412, 538
Bsp68I TCGCGA 1 cut(s) 489
BspACI CCGC 5 cut(s) 196, 243, 300, 439, 472
BspANI GGCC 8 cut(s) 45, 704, 775, 962, 1015, 1029, 1033, 1074
BspCNI CTCAG 2 cut(s) 644, 1117
BspEI TCCGGA 1 cut(s) 272
BspFNI CGCG 1 cut(s) 489
BspHI TCATGA 1 cut(s) 667
BspLI GGNNCC 5 cut(s) 255, 540, 580, 798, 963
BspPI GGATC 4 cut(s) 248, 261, 533, 546
BspT107I GGYRCC 1 cut(s) 578
BspTNI GGTCTC 1 cut(s) 862
BsrBI CCGCTC 1 cut(s) 198
BsrFI RCCGGY 1 cut(s) 1029
BsrI ACTGG 2 cut(s) 66, 610
BssAI RCCGGY 1 cut(s) 1029
BssECI CCNNGG 4 cut(s) 225, 852, 957, 1038
BssMI GATC 4 cut(s) 253, 278, 412, 538
BssNAI GTATAC 1 cut(s) 1173
BssT1I CCWWGG 2 cut(s) 225, 852
Bst1107I GTATAC 1 cut(s) 1173
Bst2UI CCWGG 2 cut(s) 583, 959
Bst4CI ACNGT 3 cut(s) 26, 710, 725
Bst6I CTCTTC 2 cut(s) 126, 207
BstAPI GCANNNNNTGC 1 cut(s) 319
BstBAI YACGTR 1 cut(s) 946
BstC8I GCNNGC 4 cut(s) 7, 437, 702, 1031
BstDEI CTNAG 6 cut(s) 47, 186, 631, 977, 1104, 1239
BstDSI CCRYGG 1 cut(s) 1038
BstF5I GGATG 3 cut(s) 121, 880, 1034
BstFNI CGCG 1 cut(s) 489
BstHHI GCGC 2 cut(s) 337, 951
BstKTI GATC 4 cut(s) 256, 281, 415, 541
BstMAI GTCTC 1 cut(s) 862
BstMBI GATC 4 cut(s) 253, 278, 412, 538
BstMWI GCNNNNNNNGC 6 cut(s) 297, 319, 445, 557, 1108, 1163
BstNI CCWGG 2 cut(s) 583, 959
BstNSI RCATGY 1 cut(s) 1179
BstSCI CCNGG 2 cut(s) 581, 957
BstSFI CTRYAG 1 cut(s) 741
BstSLI GKGCMC 1 cut(s) 845
BstUI CGCG 1 cut(s) 489
BstV1I GCAGC 4 cut(s) 272, 275, 461, 544
BstV2I GAAGAC 2 cut(s) 144, 395
BstX2I RGATCY 3 cut(s) 253, 278, 538
BstYI RGATCY 3 cut(s) 253, 278, 538
BstZ17I GTATAC 1 cut(s) 1173
BsuRI GGCC 8 cut(s) 45, 704, 775, 962, 1015, 1029, 1033, 1074
BtgI CCRYGG 1 cut(s) 1038
BtrI CACGTC 1 cut(s) 1138
BtsCI GGATG 3 cut(s) 121, 880, 1034
BtsI GCAGTG 1 cut(s) 16
BtsIMutI CAGTG 3 cut(s) 16, 59, 730
BtuMI TCGCGA 1 cut(s) 489
Cac8I GCNNGC 4 cut(s) 7, 437, 702, 1031
CciI TCATGA 1 cut(s) 667
CfoI GCGC 2 cut(s) 337, 951
Cfr10I RCCGGY 1 cut(s) 1029
Cfr13I GGNCC 2 cut(s) 961, 1073
Csp6I GTAC 6 cut(s) 27, 387, 531, 579, 603, 781
CviAII CATG 4 cut(s) 419, 668, 674, 1176
CviQI GTAC 6 cut(s) 27, 387, 531, 579, 603, 781
DdeI CTNAG 6 cut(s) 47, 186, 631, 977, 1104, 1239
DpnI GATC 4 cut(s) 255, 280, 414, 540
DpnII GATC 4 cut(s) 253, 278, 412, 538
DraI TTTAAA 1 cut(s) 40
EaeI YGGCCR 4 cut(s) 702, 773, 1027, 1031
Eam1104I CTCTTC 2 cut(s) 126, 207
EarI CTCTTC 2 cut(s) 126, 207
Eco130I CCWWGG 2 cut(s) 225, 852
Eco147I AGGCCT 1 cut(s) 45
Eco24I GRGCYC 1 cut(s) 450
Eco31I GGTCTC 1 cut(s) 862
Eco57I CTGAAG 1 cut(s) 308
Eco72I CACGTG 1 cut(s) 946
EcoRI GAATTC 1 cut(s) 1217
EcoRII CCWGG 2 cut(s) 581, 957
EcoT14I CCWWGG 2 cut(s) 225, 852
EcoT38I GRGCYC 1 cut(s) 450
ErhI CCWWGG 2 cut(s) 225, 852
FaeI CATG 4 cut(s) 422, 671, 677, 1179
FaqI GGGAC 1 cut(s) 455
FatI CATG 4 cut(s) 418, 667, 673, 1175
FauI CCCGC 3 cut(s) 203, 250, 432
FauNDI CATATG 1 cut(s) 296
FbaI TGATCA 1 cut(s) 412
FblI GTMKAC 1 cut(s) 1172
Fnu4HI GCNGC 4 cut(s) 286, 289, 475, 558
FokI GGATG 3 cut(s) 128, 887, 1021
FriOI GRGCYC 1 cut(s) 450
FseI GGCCGGCC 1 cut(s) 1033
Fsp4HI GCNGC 4 cut(s) 286, 289, 475, 558
FspAI RTGCGCAY 1 cut(s) 950
FspBI CTAG 2 cut(s) 453, 861
FspI TGCGCA 1 cut(s) 950
GlaI GCGC 2 cut(s) 336, 950
GluI GCNGC 4 cut(s) 286, 289, 475, 558
GsaI CCCAGC 1 cut(s) 934
GsuI CTGGAG 2 cut(s) 252, 573
HaeIII GGCC 8 cut(s) 45, 704, 775, 962, 1015, 1029, 1033, 1074
HapII CCGG 3 cut(s) 273, 996, 1030
HhaI GCGC 2 cut(s) 337, 951
Hin1II CATG 4 cut(s) 422, 671, 677, 1179
Hin6I GCGC 2 cut(s) 335, 949
HinP1I GCGC 2 cut(s) 335, 949
HincII GTYRAC 1 cut(s) 683
HindII GTYRAC 1 cut(s) 683
HinfI GANTC 4 cut(s) 51, 350, 694, 1142
HpaII CCGG 3 cut(s) 273, 996, 1030
HphI GGTGA 5 cut(s) 32, 314, 353, 923, 929
Hpy166II GTNNAC 7 cut(s) 305, 387, 596, 683, 1141, 1173, 1207
Hpy188I TCNGA 3 cut(s) 519, 634, 1095
Hpy188III TCNNGA 7 cut(s) 257, 273, 410, 453, 488, 668, 1148
Hpy8I GTNNAC 7 cut(s) 305, 387, 596, 683, 1141, 1173, 1207
Hpy99I CGWCG 3 cut(s) 80, 411, 1139
HpyAV CCTTC 4 cut(s) 340, 457, 517, 580
HpyCH4III ACNGT 3 cut(s) 26, 710, 725
HpyCH4IV ACGT 4 cut(s) 389, 945, 1125, 1137
HpyCH4V TGCA 6 cut(s) 313, 356, 560, 612, 758, 1196
HpyF10VI GCNNNNNNNGC 6 cut(s) 297, 319, 445, 557, 1108, 1163
HpyF3I CTNAG 6 cut(s) 47, 186, 631, 977, 1104, 1239
HpySE526I ACGT 4 cut(s) 389, 945, 1125, 1137
Hsp92II CATG 4 cut(s) 422, 671, 677, 1179
HspAI GCGC 2 cut(s) 335, 949
Kpn2I TCCGGA 1 cut(s) 272
KpnI GGTACC 1 cut(s) 582
KroI GCCGGC 1 cut(s) 1029
KroNI GCCGGC 1 cut(s) 1031
Ksp22I TGATCA 1 cut(s) 412
Kzo9I GATC 4 cut(s) 253, 278, 412, 538
LmnI GCTCC 2 cut(s) 554, 796
Lsp1109I GCAGC 4 cut(s) 272, 275, 461, 544
LweI GCATC 2 cut(s) 813, 1183
MaeI CTAG 2 cut(s) 453, 861
MaeII ACGT 4 cut(s) 389, 945, 1125, 1137
MaeIII GTNAC 2 cut(s) 20, 863
MalI GATC 4 cut(s) 255, 280, 414, 540
MbiI CCGCTC 1 cut(s) 198
MboI GATC 4 cut(s) 253, 278, 412, 538
MflI RGATCY 3 cut(s) 253, 278, 538
MhlI GDGCHC 2 cut(s) 450, 845
MlsI TGGCCA 1 cut(s) 775
MluCI AATT 9 cut(s) 154, 314, 479, 568, 786, 847, 901, 1088, 1217
MluNI TGGCCA 1 cut(s) 775
MlyI GAGTC 1 cut(s) 1136
Mox20I TGGCCA 1 cut(s) 775
MroI TCCGGA 1 cut(s) 272
MroNI GCCGGC 1 cut(s) 1029
MroXI GAANNNNTTC 1 cut(s) 467
MscI TGGCCA 1 cut(s) 775
MseI TTAA 1 cut(s) 39
MslI CAYNNNNRTG 2 cut(s) 672, 821
Msp20I TGGCCA 1 cut(s) 775
MspA1I CMGCKG 3 cut(s) 439, 474, 700
MspI CCGG 3 cut(s) 273, 996, 1030
MspR9I CCNGG 2 cut(s) 583, 959
Mva1269I GAATGC 1 cut(s) 652
MvaI CCWGG 2 cut(s) 583, 959
MvnI CGCG 1 cut(s) 489
MwoI GCNNNNNNNGC 6 cut(s) 297, 319, 445, 557, 1108, 1163
NaeI GCCGGC 1 cut(s) 1031
NdeI CATATG 1 cut(s) 296
NdeII GATC 4 cut(s) 253, 278, 412, 538
NgoMIV GCCGGC 1 cut(s) 1029
NlaIII CATG 4 cut(s) 422, 671, 677, 1179
NlaIV GGNNCC 5 cut(s) 255, 540, 580, 798, 963
NmuCI GTSAC 1 cut(s) 20
NruI TCGCGA 1 cut(s) 489
NsbI TGCGCA 1 cut(s) 950
NspI RCATGY 1 cut(s) 1179
PagI TCATGA 1 cut(s) 667
PceI AGGCCT 1 cut(s) 45
PciI ACATGT 1 cut(s) 1175
PcsI WCGNNNNNNNCGW 3 cut(s) 72, 395, 1131
PctI GAATGC 1 cut(s) 652
PdiI GCCGGC 1 cut(s) 1031
PdmI GAANNNNTTC 1 cut(s) 467
PfeI GAWTC 3 cut(s) 51, 350, 694
PkrI GCNGC 4 cut(s) 287, 290, 476, 559
PleI GAGTC 1 cut(s) 1136
PmaCI CACGTG 1 cut(s) 946
PmlI CACGTG 1 cut(s) 946
PpsI GAGTC 1 cut(s) 1136
Ppu21I YACGTR 1 cut(s) 946
PscI ACATGT 1 cut(s) 1175
Psp6I CCWGG 2 cut(s) 581, 957
PspCI CACGTG 1 cut(s) 946
PspFI CCCAGC 1 cut(s) 930
PspGI CCWGG 2 cut(s) 581, 957
PspN4I GGNNCC 5 cut(s) 255, 540, 580, 798, 963
PspPI GGNCC 2 cut(s) 961, 1073
PsuI RGATCY 3 cut(s) 253, 278, 538
PvuII CAGCTG 1 cut(s) 700
RigI GGCCGGCC 1 cut(s) 1033
RruI TCGCGA 1 cut(s) 489
RsaI GTAC 6 cut(s) 28, 388, 532, 580, 604, 782
RsaNI GTAC 6 cut(s) 27, 387, 531, 579, 603, 781
RseI CAYNNNNRTG 2 cut(s) 672, 821
SaqAI TTAA 1 cut(s) 39
SatI GCNGC 4 cut(s) 286, 289, 475, 558
Sau3AI GATC 4 cut(s) 253, 278, 412, 538
Sau96I GGNCC 2 cut(s) 961, 1073
SchI GAGTC 1 cut(s) 1136
ScrFI CCNGG 2 cut(s) 583, 959
SduI GDGCHC 2 cut(s) 450, 845
SfaNI GCATC 2 cut(s) 813, 1183
SfcI CTRYAG 1 cut(s) 741
SmiMI CAYNNNNRTG 2 cut(s) 672, 821
SmlI CTYRAG 1 cut(s) 505
SmoI CTYRAG 1 cut(s) 505
Sse9I AATT 9 cut(s) 154, 314, 479, 568, 786, 847, 901, 1088, 1217
SseBI AGGCCT 1 cut(s) 45
SsiI CCGC 5 cut(s) 196, 243, 300, 439, 472
SspMI CTAG 2 cut(s) 453, 861
StuI AGGCCT 1 cut(s) 45
StyD4I CCNGG 2 cut(s) 581, 957
StyI CCWWGG 2 cut(s) 225, 852
TaaI ACNGT 3 cut(s) 26, 710, 725
TaiI ACGT 4 cut(s) 392, 948, 1128, 1140
TaqI TCGA 1 cut(s) 890
TaqII GACCGA 1 cut(s) 648
TasI AATT 9 cut(s) 154, 314, 479, 568, 786, 847, 901, 1088, 1217
TatI WGTACW 2 cut(s) 602, 780
TfiI GAWTC 3 cut(s) 51, 350, 694
Tru1I TTAA 1 cut(s) 39
Tru9I TTAA 1 cut(s) 39
TscAI CASTG 3 cut(s) 16, 66, 730
TseFI GTSAC 1 cut(s) 20
TseI GCWGC 4 cut(s) 285, 288, 474, 557
Tsp45I GTSAC 1 cut(s) 20
TspDTI ATGAA 4 cut(s) 435, 684, 798, 927
TspGWI ACGGA 3 cut(s) 44, 548, 833
TspRI CASTG 3 cut(s) 16, 66, 730
XapI RAATTY 3 cut(s) 154, 1088, 1217
XbaI TCTAGA 1 cut(s) 452
XceI RCATGY 1 cut(s) 1179
XmiI GTMKAC 1 cut(s) 1172
XmnI GAANNNNTTC 1 cut(s) 467
XspI CTAG 2 cut(s) 453, 861
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.