MD10G1217600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0012388
Physical Location & Seq
Forward (+)
31603302 .. 31605074
1773 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1217600.v1.1.491

Sequence Viewer

Length: 474 bp
ATGAAAGCCCAATTCAACCTGAACAGAAGGAAGAAGGAACCAAAAACCAGAGAAGGCAGAAGAGACGGAGCGACGAGCTGGAAGTCGAGAATGTCGATACTGTGGGAGAAGAGCGGGACATGGAGGTGGATAGTGAACAAGACCCGTGACTCGAAGCCCTTCTTCTTGGCCTTCGCCACCGTCTGCGGCGTCGTTCCGGGCGTTATTGGCTACTTCGTTATGCAGACGACCAACTCCGGAAACCAACAACTCGAGGCCGAGCTCCGCCGCAACGCCCGACCTGAATCCATGAGGATGGGGCAAGTGAATAAAGAAAGATTGGCAGAATTCCTTGGGGAGTTGCAGCGGAAAGAGAACACAAATGACCGCTATGTTGCTGCACTGAAAGGAGAGACATTGACTAGGAATCCATATGTGAGAATTCAACCAATTCCAAAGCCAAGCAACACCGAAGCTGAGAAGGAACAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

158

Amino Acids

18.23

Weight (kDa)

10.34

Isoelectric Point (pI)

40.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012388)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79390
fragaria_vesca FvH4_3g13970
malus_domestica MD05G1239400.v1.1 MD10G1217600.v1.1
prunus_persica Prupe.4G126700_v2.0.a1
pyrus_communis pycom05g21570 pycom10g18350
rosa_chinensis RchiOBHm_Chr5g0023201
rosa_laevigata RLG00000032708
rosa_multiflora Rmu_sc0007300.1_g000013
rosa_roxburghii Rroxscaffold_1G00056150
rosa_rugosa Rorug05G0077700 Rorug05G0077800.1 Rorug05G0077900
rosa_samantha Rh5AG164000 Rh5BG163400 Rh5CG178700 Rh5DG165300
rosa_wichuraiana Rw5G014800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 114
AccIII TCCGGA 1 cut(s) 236
AciI CCGC 6 cut(s) 114, 186, 265, 268, 346, 367
AcsI RAATTY 2 cut(s) 326, 420
AcyI GRCGYC 1 cut(s) 189
AgsI TTSAA 2 cut(s) 16, 425
AluBI AGCT 3 cut(s) 78, 262, 455
AluI AGCT 3 cut(s) 78, 262, 455
Alw21I GWGCWC 1 cut(s) 264
Alw26I GTCTC 2 cut(s) 57, 386
Ama87I CYCGRG 1 cut(s) 251
Aor13HI TCCGGA 1 cut(s) 236
AoxI GGCC 2 cut(s) 168, 255
ApeKI GCWGC 2 cut(s) 343, 377
ApoI RAATTY 2 cut(s) 326, 420
Asp700I GAANNNNTTC 1 cut(s) 158
AsuC2I CCSGG 1 cut(s) 198
AvaI CYCGRG 1 cut(s) 251
BanII GRGCYC 1 cut(s) 264
Bbv12I GWGCWC 1 cut(s) 264
BbvI GCAGC 2 cut(s) 355, 364
BccI CCATC 1 cut(s) 289
BcnI CCSGG 1 cut(s) 198
BcoDI GTCTC 2 cut(s) 57, 386
BfaI CTAG 1 cut(s) 402
BisI GCNGC 4 cut(s) 187, 268, 344, 378
BlsI GCNGC 4 cut(s) 188, 269, 345, 379
Bme1390I CCNGG 1 cut(s) 198
BmeT110I CYCGRG 1 cut(s) 251
BmiI GGNNCC 1 cut(s) 39
BmrFI CCNGG 1 cut(s) 198
BpuMI CCSGG 1 cut(s) 198
BsaHI GRCGYC 1 cut(s) 189
BsaJI CCNNGG 1 cut(s) 331
BsaWI WCCGGW 1 cut(s) 236
BseAI TCCGGA 1 cut(s) 236
BseDI CCNNGG 1 cut(s) 331
BseGI GGATG 1 cut(s) 300
BseMII CTCAG 1 cut(s) 447
BseXI GCAGC 2 cut(s) 355, 364
BsgI GTGCAG 1 cut(s) 363
BshFI GGCC 2 cut(s) 170, 257
BsiHKAI GWGCWC 1 cut(s) 264
BsiHKCI CYCGRG 1 cut(s) 251
BsiSI CCGG 2 cut(s) 197, 237
BslFI GGGAC 1 cut(s) 130
BsmAI GTCTC 2 cut(s) 57, 386
BsmBI CGTCTC 1 cut(s) 57
BsmFI GGGAC 1 cut(s) 130
BsnI GGCC 2 cut(s) 170, 257
BsoBI CYCGRG 1 cut(s) 251
Bsp1286I GDGCHC 1 cut(s) 264
Bsp13I TCCGGA 1 cut(s) 236
BspACI CCGC 6 cut(s) 114, 186, 265, 268, 346, 367
BspANI GGCC 2 cut(s) 170, 257
BspCNI CTCAG 1 cut(s) 448
BspEI TCCGGA 1 cut(s) 236
BspLI GGNNCC 1 cut(s) 39
BspQI GCTCTTC 1 cut(s) 104
BsrBI CCGCTC 1 cut(s) 114
BssECI CCNNGG 1 cut(s) 331
BssNI GRCGYC 1 cut(s) 189
BssT1I CCWWGG 1 cut(s) 331
Bst4CI ACNGT 2 cut(s) 102, 181
Bst6I CTCTTC 2 cut(s) 55, 104
BstACI GRCGYC 1 cut(s) 189
BstDEI CTNAG 1 cut(s) 456
BstF5I GGATG 1 cut(s) 300
BstMAI GTCTC 2 cut(s) 57, 386
BstMWI GCNNNNNNNGC 1 cut(s) 207
BstSCI CCNGG 1 cut(s) 196
BstV1I GCAGC 2 cut(s) 355, 364
BstXI CCANNNNNNTGG 1 cut(s) 295
BsuRI GGCC 2 cut(s) 170, 257
BtsCI GGATG 1 cut(s) 300
BtsIMutI CAGTG 1 cut(s) 380
CseI GACGC 1 cut(s) 178
CviAII CATG 2 cut(s) 120, 289
CviJI RGCY 9 cut(s) 8, 78, 157, 170, 210, 257, 262, 439, 455
CviKI_1 RGCY 9 cut(s) 8, 78, 157, 170, 210, 257, 262, 439, 455
DdeI CTNAG 1 cut(s) 456
Eam1104I CTCTTC 2 cut(s) 55, 104
EarI CTCTTC 2 cut(s) 55, 104
EciI GGCGGA 1 cut(s) 254
Ecl136II GAGCTC 1 cut(s) 262
Eco130I CCWWGG 1 cut(s) 331
Eco24I GRGCYC 1 cut(s) 264
Eco53kI GAGCTC 1 cut(s) 262
Eco88I CYCGRG 1 cut(s) 251
EcoICRI GAGCTC 1 cut(s) 262
EcoRI GAATTC 2 cut(s) 326, 420
EcoT14I CCWWGG 1 cut(s) 331
EcoT38I GRGCYC 1 cut(s) 264
ErhI CCWWGG 1 cut(s) 331
Esp3I CGTCTC 1 cut(s) 57
FaeI CATG 2 cut(s) 123, 292
FaiI YATR 6 cut(s) 121, 221, 290, 372, 412, 414
FalI AAGNNNNNCTT 2 cut(s) 146, 178
FaqI GGGAC 1 cut(s) 130
FatI CATG 2 cut(s) 119, 288
FauI CCCGC 1 cut(s) 107
FauNDI CATATG 1 cut(s) 412
Fnu4HI GCNGC 4 cut(s) 187, 268, 344, 378
FokI GGATG 1 cut(s) 307
FriOI GRGCYC 1 cut(s) 264
Fsp4HI GCNGC 4 cut(s) 187, 268, 344, 378
FspBI CTAG 1 cut(s) 402
GluI GCNGC 4 cut(s) 187, 268, 344, 378
HaeIII GGCC 2 cut(s) 170, 257
HapII CCGG 2 cut(s) 197, 237
HgaI GACGC 1 cut(s) 178
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 2 cut(s) 123, 292
HinfI GANTC 3 cut(s) 149, 284, 406
HpaII CCGG 2 cut(s) 197, 237
Hpy166II GTNNAC 1 cut(s) 136
Hpy188III TCNNGA 2 cut(s) 87, 237
Hpy8I GTNNAC 1 cut(s) 136
Hpy99I CGWCG 2 cut(s) 76, 194
HpyAV CCTTC 6 cut(s) 21, 28, 47, 169, 181, 454
HpyCH4III ACNGT 2 cut(s) 102, 181
HpyCH4V TGCA 3 cut(s) 223, 343, 380
HpyF10VI GCNNNNNNNGC 1 cut(s) 207
HpyF3I CTNAG 1 cut(s) 456
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 2 cut(s) 123, 292
Kpn2I TCCGGA 1 cut(s) 236
LguI GCTCTTC 1 cut(s) 104
LmnI GCTCC 2 cut(s) 68, 267
LpnPI CCDG 6 cut(s) 32, 61, 64, 210, 250, 294
Lsp1109I GCAGC 2 cut(s) 355, 364
MaeI CTAG 1 cut(s) 402
MaeIII GTNAC 1 cut(s) 146
MbiI CCGCTC 1 cut(s) 114
MboII GAAGA 4 cut(s) 43, 72, 121, 154
MhlI GDGCHC 1 cut(s) 264
MluCI AATT 4 cut(s) 11, 326, 420, 429
MlyI GAGTC 1 cut(s) 143
MnlI CCTC 3 cut(s) 117, 247, 285
MroI TCCGGA 1 cut(s) 236
MroXI GAANNNNTTC 1 cut(s) 158
MslI CAYNNNNRTG 2 cut(s) 124, 293
MspA1I CMGCKG 1 cut(s) 346
MspI CCGG 2 cut(s) 197, 237
MspR9I CCNGG 1 cut(s) 198
MwoI GCNNNNNNNGC 1 cut(s) 207
NciI CCSGG 1 cut(s) 198
NdeI CATATG 1 cut(s) 412
NlaIII CATG 2 cut(s) 123, 292
NlaIV GGNNCC 1 cut(s) 39
NmeAIII GCCGAG 1 cut(s) 283
NmuCI GTSAC 1 cut(s) 146
PaeR7I CTCGAG 1 cut(s) 251
PciSI GCTCTTC 1 cut(s) 104
PcsI WCGNNNNNNNCGW 2 cut(s) 92, 198
PdmI GAANNNNTTC 1 cut(s) 158
PfeI GAWTC 2 cut(s) 284, 406
PkrI GCNGC 4 cut(s) 188, 269, 345, 379
PleI GAGTC 1 cut(s) 143
PpsI GAGTC 1 cut(s) 143
Psp124BI GAGCTC 1 cut(s) 264
PspN4I GGNNCC 1 cut(s) 39
PspXI VCTCGAGB 1 cut(s) 251
RseI CAYNNNNRTG 2 cut(s) 124, 293
SacI GAGCTC 1 cut(s) 264
SapI GCTCTTC 1 cut(s) 104
SatI GCNGC 4 cut(s) 187, 268, 344, 378
SchI GAGTC 1 cut(s) 143
ScrFI CCNGG 1 cut(s) 198
SduI GDGCHC 1 cut(s) 264
SetI ASST 6 cut(s) 21, 80, 128, 264, 283, 457
Sfr274I CTCGAG 1 cut(s) 251
SlaI CTCGAG 1 cut(s) 251
SmiMI CAYNNNNRTG 2 cut(s) 124, 293
SmlI CTYRAG 1 cut(s) 251
SmoI CTYRAG 1 cut(s) 251
Sse9I AATT 4 cut(s) 11, 326, 420, 429
SsiI CCGC 6 cut(s) 114, 186, 265, 268, 346, 367
SspMI CTAG 1 cut(s) 402
SstI GAGCTC 1 cut(s) 264
StyD4I CCNGG 1 cut(s) 196
StyI CCWWGG 1 cut(s) 331
TaaI ACNGT 2 cut(s) 102, 181
TaqI TCGA 4 cut(s) 86, 95, 152, 252
TasI AATT 4 cut(s) 11, 326, 420, 429
TauI GCSGC 2 cut(s) 189, 270
TfiI GAWTC 2 cut(s) 284, 406
TscAI CASTG 1 cut(s) 387
TseFI GTSAC 1 cut(s) 146
TseI GCWGC 2 cut(s) 343, 377
Tsp45I GTSAC 1 cut(s) 146
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 81
TspRI CASTG 1 cut(s) 387
XapI RAATTY 2 cut(s) 326, 420
XhoI CTCGAG 1 cut(s) 251
XmnI GAANNNNTTC 1 cut(s) 158
XspI CTAG 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.