pycom10g18350

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0012388
Physical Location & Seq
Forward (+)
21654548 .. 21656058
1511 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g18350.1

Sequence Viewer

Length: 489 bp
ATGGGCCTTACAGGCCATGTAAAAAAGACACGTGTTAAACAGAAGGAAAAAGGAACCAAAAACCAGAGAAGGCAGAAGAGACGCAGCGACGAGCTGAAGTCGAGAATGTCGATACTGTGGGAGAAGAGCGGGACATGGAGGTGGGTAGTGAACAAGACCCGTGATTCGAAGCCCTTCTTCTTGGCCTTCGCCACCGTCTGCGGCGTCGTTCCGGGCGTTATTGGCTACTTCGTTATGCAGACCACCAACTCTGGAAACCAACAGCTCGAGGCCGAGCTCCGCCGCAACGCCCGACCTGAATCCATGAGGATGGGGCAAGTGAATAAAGAAAGATTGGCAGAATTCCTTGGGGAGTTGCAGCGGAAAGAGAACACAAATGACCGCTATGTTGCTGCACTGAAAGGAGAGACATTGACTAGAAATCCATATGTGAGAATTCAACCAATTCCAAAGCCAAGCAACACCGAAGCTGAGAAGGAACAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

163

Amino Acids

18.78

Weight (kDa)

10.43

Isoelectric Point (pI)

43.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012388)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79390
fragaria_vesca FvH4_3g13970
malus_domestica MD05G1239400.v1.1 MD10G1217600.v1.1
prunus_persica Prupe.4G126700_v2.0.a1
pyrus_communis pycom05g21570 pycom10g18350
rosa_chinensis RchiOBHm_Chr5g0023201
rosa_laevigata RLG00000032708
rosa_multiflora Rmu_sc0007300.1_g000013
rosa_roxburghii Rroxscaffold_1G00056150
rosa_rugosa Rorug05G0077700 Rorug05G0077800.1 Rorug05G0077900
rosa_samantha Rh5AG164000 Rh5BG163400 Rh5CG178700 Rh5DG165300
rosa_wichuraiana Rw5G014800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 129
AciI CCGC 6 cut(s) 129, 201, 280, 283, 361, 382
AcsI RAATTY 2 cut(s) 341, 435
AcuI CTGAAG 1 cut(s) 116
AcvI CACGTG 1 cut(s) 32
AcyI GRCGYC 1 cut(s) 204
AflIII ACRYGT 2 cut(s) 29, 31
AgsI TTSAA 1 cut(s) 440
AluBI AGCT 4 cut(s) 94, 265, 277, 470
AluI AGCT 4 cut(s) 94, 265, 277, 470
Alw21I GWGCWC 1 cut(s) 279
Alw26I GTCTC 2 cut(s) 73, 401
Ama87I CYCGRG 1 cut(s) 266
AoxI GGCC 4 cut(s) 4, 13, 183, 270
ApeKI GCWGC 3 cut(s) 84, 358, 392
ApoI RAATTY 2 cut(s) 341, 435
ArsI GACNNNNNNTTYG 2 cut(s) 148, 180
Asp700I GAANNNNTTC 1 cut(s) 173
AspS9I GGNCC 1 cut(s) 4
AsuC2I CCSGG 1 cut(s) 213
AsuII TTCGAA 1 cut(s) 167
AvaI CYCGRG 1 cut(s) 266
BanII GRGCYC 1 cut(s) 279
BbrPI CACGTG 1 cut(s) 32
Bbv12I GWGCWC 1 cut(s) 279
BbvI GCAGC 3 cut(s) 96, 370, 379
BccI CCATC 1 cut(s) 304
BcnI CCSGG 1 cut(s) 213
BcoDI GTCTC 2 cut(s) 73, 401
BfaI CTAG 1 cut(s) 417
BglI GCCNNNNNGGC 1 cut(s) 12
BisI GCNGC 5 cut(s) 85, 202, 283, 359, 393
BlsI GCNGC 5 cut(s) 86, 203, 284, 360, 394
Bme1390I CCNGG 1 cut(s) 213
BmeT110I CYCGRG 1 cut(s) 266
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 1 cut(s) 55
BmrFI CCNGG 1 cut(s) 213
Bpu14I TTCGAA 1 cut(s) 167
BpuMI CCSGG 1 cut(s) 213
BsaAI YACGTR 1 cut(s) 32
BsaHI GRCGYC 1 cut(s) 204
BsaJI CCNNGG 1 cut(s) 346
BseDI CCNNGG 1 cut(s) 346
BseGI GGATG 1 cut(s) 315
BseMII CTCAG 1 cut(s) 462
BseXI GCAGC 3 cut(s) 96, 370, 379
BsgI GTGCAG 1 cut(s) 378
BshFI GGCC 4 cut(s) 6, 15, 185, 272
BsiHKAI GWGCWC 1 cut(s) 279
BsiHKCI CYCGRG 1 cut(s) 266
BsiSI CCGG 1 cut(s) 212
BslFI GGGAC 1 cut(s) 145
BsmAI GTCTC 2 cut(s) 73, 401
BsmBI CGTCTC 1 cut(s) 73
BsmFI GGGAC 1 cut(s) 145
BsnI GGCC 4 cut(s) 6, 15, 185, 272
BsoBI CYCGRG 1 cut(s) 266
Bsp119I TTCGAA 1 cut(s) 167
Bsp1286I GDGCHC 1 cut(s) 279
BspACI CCGC 6 cut(s) 129, 201, 280, 283, 361, 382
BspANI GGCC 4 cut(s) 6, 15, 185, 272
BspCNI CTCAG 1 cut(s) 463
BspLI GGNNCC 1 cut(s) 55
BspQI GCTCTTC 1 cut(s) 119
BspT104I TTCGAA 1 cut(s) 167
BsrBI CCGCTC 1 cut(s) 129
BssECI CCNNGG 1 cut(s) 346
BssNI GRCGYC 1 cut(s) 204
BssT1I CCWWGG 1 cut(s) 346
Bst4CI ACNGT 2 cut(s) 117, 196
Bst6I CTCTTC 2 cut(s) 71, 119
BstACI GRCGYC 1 cut(s) 204
BstBAI YACGTR 1 cut(s) 32
BstBI TTCGAA 1 cut(s) 167
BstDEI CTNAG 1 cut(s) 471
BstF5I GGATG 1 cut(s) 315
BstMAI GTCTC 2 cut(s) 73, 401
BstMWI GCNNNNNNNGC 2 cut(s) 12, 222
BstSCI CCNGG 1 cut(s) 211
BstV1I GCAGC 3 cut(s) 96, 370, 379
BstXI CCANNNNNNTGG 1 cut(s) 310
BsuRI GGCC 4 cut(s) 6, 15, 185, 272
BtsCI GGATG 1 cut(s) 315
BtsIMutI CAGTG 1 cut(s) 395
Cfr13I GGNCC 1 cut(s) 4
CseI GACGC 2 cut(s) 90, 193
CviAII CATG 3 cut(s) 17, 135, 304
DdeI CTNAG 1 cut(s) 471
Eam1104I CTCTTC 2 cut(s) 71, 119
EarI CTCTTC 2 cut(s) 71, 119
EciI GGCGGA 1 cut(s) 269
Ecl136II GAGCTC 1 cut(s) 277
Eco130I CCWWGG 1 cut(s) 346
Eco24I GRGCYC 1 cut(s) 279
Eco53kI GAGCTC 1 cut(s) 277
Eco57I CTGAAG 1 cut(s) 116
Eco72I CACGTG 1 cut(s) 32
Eco88I CYCGRG 1 cut(s) 266
EcoICRI GAGCTC 1 cut(s) 277
EcoRI GAATTC 2 cut(s) 341, 435
EcoT14I CCWWGG 1 cut(s) 346
EcoT38I GRGCYC 1 cut(s) 279
ErhI CCWWGG 1 cut(s) 346
Esp3I CGTCTC 1 cut(s) 73
FaeI CATG 3 cut(s) 20, 138, 307
FaiI YATR 7 cut(s) 18, 136, 236, 305, 387, 427, 429
FalI AAGNNNNNCTT 2 cut(s) 161, 193
FaqI GGGAC 1 cut(s) 145
FatI CATG 3 cut(s) 16, 134, 303
FauI CCCGC 1 cut(s) 122
FauNDI CATATG 1 cut(s) 427
Fnu4HI GCNGC 5 cut(s) 85, 202, 283, 359, 393
FokI GGATG 1 cut(s) 322
FriOI GRGCYC 1 cut(s) 279
Fsp4HI GCNGC 5 cut(s) 85, 202, 283, 359, 393
FspBI CTAG 1 cut(s) 417
GluI GCNGC 5 cut(s) 85, 202, 283, 359, 393
HaeIII GGCC 4 cut(s) 6, 15, 185, 272
HapII CCGG 1 cut(s) 212
HgaI GACGC 2 cut(s) 90, 193
Hin1I GRCGYC 1 cut(s) 204
Hin1II CATG 3 cut(s) 20, 138, 307
HinfI GANTC 2 cut(s) 164, 299
HpaII CCGG 1 cut(s) 212
Hpy166II GTNNAC 1 cut(s) 151
Hpy188III TCNNGA 2 cut(s) 102, 252
Hpy8I GTNNAC 1 cut(s) 151
Hpy99I CGWCG 2 cut(s) 92, 209
HpyAV CCTTC 5 cut(s) 37, 63, 184, 196, 469
HpyCH4III ACNGT 2 cut(s) 117, 196
HpyCH4IV ACGT 1 cut(s) 31
HpyCH4V TGCA 3 cut(s) 238, 358, 395
HpyF10VI GCNNNNNNNGC 2 cut(s) 12, 222
HpyF3I CTNAG 1 cut(s) 471
HpySE526I ACGT 1 cut(s) 31
Hsp92I GRCGYC 1 cut(s) 204
Hsp92II CATG 3 cut(s) 20, 138, 307
LguI GCTCTTC 1 cut(s) 119
LmnI GCTCC 1 cut(s) 282
LpnPI CCDG 4 cut(s) 77, 225, 237, 309
Lsp1109I GCAGC 3 cut(s) 96, 370, 379
MaeI CTAG 1 cut(s) 417
MaeII ACGT 1 cut(s) 31
MbiI CCGCTC 1 cut(s) 129
MboII GAAGA 3 cut(s) 88, 136, 169
MhlI GDGCHC 1 cut(s) 279
MluCI AATT 3 cut(s) 341, 435, 444
MnlI CCTC 3 cut(s) 132, 262, 300
MroXI GAANNNNTTC 1 cut(s) 173
MseI TTAA 1 cut(s) 36
MslI CAYNNNNRTG 2 cut(s) 139, 308
MspA1I CMGCKG 1 cut(s) 361
MspI CCGG 1 cut(s) 212
MspR9I CCNGG 1 cut(s) 213
MwoI GCNNNNNNNGC 2 cut(s) 12, 222
NciI CCSGG 1 cut(s) 213
NdeI CATATG 1 cut(s) 427
NlaIII CATG 3 cut(s) 20, 138, 307
NlaIV GGNNCC 1 cut(s) 55
NmeAIII GCCGAG 1 cut(s) 298
NspV TTCGAA 1 cut(s) 167
PaeR7I CTCGAG 1 cut(s) 266
PciSI GCTCTTC 1 cut(s) 119
PcsI WCGNNNNNNNCGW 2 cut(s) 107, 213
PdmI GAANNNNTTC 1 cut(s) 173
PfeI GAWTC 2 cut(s) 164, 299
PkrI GCNGC 5 cut(s) 86, 203, 284, 360, 394
PmaCI CACGTG 1 cut(s) 32
PmlI CACGTG 1 cut(s) 32
Ppu21I YACGTR 1 cut(s) 32
Psp124BI GAGCTC 1 cut(s) 279
PspCI CACGTG 1 cut(s) 32
PspN4I GGNNCC 1 cut(s) 55
PspPI GGNCC 1 cut(s) 4
PspXI VCTCGAGB 1 cut(s) 266
RseI CAYNNNNRTG 2 cut(s) 139, 308
SacI GAGCTC 1 cut(s) 279
SapI GCTCTTC 1 cut(s) 119
SaqAI TTAA 1 cut(s) 36
SatI GCNGC 5 cut(s) 85, 202, 283, 359, 393
Sau96I GGNCC 1 cut(s) 4
ScrFI CCNGG 1 cut(s) 213
SduI GDGCHC 1 cut(s) 279
SetI ASST 7 cut(s) 34, 96, 143, 267, 279, 298, 472
SfiI GGCCNNNNNGGCC 1 cut(s) 12
Sfr274I CTCGAG 1 cut(s) 266
SfuI TTCGAA 1 cut(s) 167
SlaI CTCGAG 1 cut(s) 266
SmiMI CAYNNNNRTG 2 cut(s) 139, 308
SmlI CTYRAG 1 cut(s) 266
SmoI CTYRAG 1 cut(s) 266
Sse9I AATT 3 cut(s) 341, 435, 444
SsiI CCGC 6 cut(s) 129, 201, 280, 283, 361, 382
SspMI CTAG 1 cut(s) 417
SstI GAGCTC 1 cut(s) 279
StyD4I CCNGG 1 cut(s) 211
StyI CCWWGG 1 cut(s) 346
TaaI ACNGT 2 cut(s) 117, 196
TaiI ACGT 1 cut(s) 34
TaqI TCGA 4 cut(s) 101, 110, 167, 267
TasI AATT 3 cut(s) 341, 435, 444
TauI GCSGC 2 cut(s) 204, 285
TfiI GAWTC 2 cut(s) 164, 299
Tru1I TTAA 1 cut(s) 36
Tru9I TTAA 1 cut(s) 36
TscAI CASTG 1 cut(s) 402
TseI GCWGC 3 cut(s) 84, 358, 392
TspRI CASTG 1 cut(s) 402
XapI RAATTY 2 cut(s) 341, 435
XhoI CTCGAG 1 cut(s) 266
XmnI GAANNNNTTC 1 cut(s) 173
XspI CTAG 1 cut(s) 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.