pycom05g21570

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0012388
Physical Location & Seq
Forward (+)
24159078 .. 24161717
2640 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g21570.3

Sequence Viewer

Length: 603 bp
ATGATGATCTTACGTAAGGCATTGTCATTGGGCCCAAACCAATGGGCCCTACAAGGTAAAGGTTCAGACACGTGTGACACCTCAGTCCAGCTAAAGAGACCACGTGCTAAGTCTTTAGTCCACGGAAGAAAGACATGTGTGAAGTCCAATAAGAGCCCAATTCAACCTGAACAGAAGAAAAACGAACCGAAAACCAGAGAAGGCAAAAGAGACGGACCGACGAGCTTCAGGTCGAGAATGCCGATGCTGTGGAGGTGGATAGTGAACAAGACCCGTGACTCGACGCCCTTCTTCTTGGCCTTCGCCACCGTCTGCGGCGTCGTTCCGGGCGTTATTGGTTACTTCGTTATGCAGACGACCAACTCCGGAAACCAACAGCTGGAGGCCGAGCTCCGCCGCAACGCCCGACCCGAATCAATGAGGATGGGGCAAGTGAATAAAGAAAGATTGGCAGAATTCCTTGGGGAGTTGCAGCGAAAGGAGAACACAAACGATCGCTATGTTGCTGCACTGAAAGGAGAGACATTGACTAGGAATCCATATGTGAGAATTCAACCAATTCCGAAGCCAAGCAACACTGAATCTGGGAAAGAACAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

201

Amino Acids

22.73

Weight (kDa)

10.37

Isoelectric Point (pI)

40.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012388)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79390
fragaria_vesca FvH4_3g13970
malus_domestica MD05G1239400.v1.1 MD10G1217600.v1.1
prunus_persica Prupe.4G126700_v2.0.a1
pyrus_communis pycom05g21570 pycom10g18350
rosa_chinensis RchiOBHm_Chr5g0023201
rosa_laevigata RLG00000032708
rosa_multiflora Rmu_sc0007300.1_g000013
rosa_roxburghii Rroxscaffold_1G00056150
rosa_rugosa Rorug05G0077700 Rorug05G0077800.1 Rorug05G0077900
rosa_samantha Rh5AG164000 Rh5BG163400 Rh5CG178700 Rh5DG165300
rosa_wichuraiana Rw5G014800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 83
AccB7I CCANNNNNTGG 1 cut(s) 379
AccIII TCCGGA 1 cut(s) 365
AciI CCGC 3 cut(s) 315, 394, 397
AcsI RAATTY 2 cut(s) 455, 549
AcuI CTGAAG 1 cut(s) 211
AcvI CACGTG 2 cut(s) 72, 104
AcyI GRCGYC 2 cut(s) 284, 318
AfiI CCNNNNNNNGG 1 cut(s) 379
AflIII ACRYGT 3 cut(s) 69, 71, 134
AgsI TTSAA 2 cut(s) 164, 554
AluBI AGCT 4 cut(s) 91, 225, 379, 391
AluI AGCT 4 cut(s) 91, 225, 379, 391
Alw21I GWGCWC 1 cut(s) 393
Alw26I GTCTC 3 cut(s) 91, 204, 515
Aor13HI TCCGGA 1 cut(s) 365
AoxI GGCC 4 cut(s) 31, 45, 297, 384
ApaI GGGCCC 2 cut(s) 35, 49
ApeKI GCWGC 2 cut(s) 472, 506
ApoI RAATTY 2 cut(s) 455, 549
AspS9I GGNCC 5 cut(s) 31, 32, 45, 46, 215
AsuC2I CCSGG 1 cut(s) 327
AvaII GGWCC 1 cut(s) 215
BaeGI GKGCMC 2 cut(s) 35, 49
BanII GRGCYC 4 cut(s) 35, 49, 158, 393
BbrPI CACGTG 2 cut(s) 72, 104
Bbv12I GWGCWC 1 cut(s) 393
BbvI GCAGC 2 cut(s) 484, 493
BccI CCATC 1 cut(s) 418
BcnI CCSGG 1 cut(s) 327
BcoDI GTCTC 3 cut(s) 91, 204, 515
BfaI CTAG 1 cut(s) 531
BisI GCNGC 4 cut(s) 316, 397, 473, 507
BlsI GCNGC 4 cut(s) 317, 398, 474, 508
Bme1390I CCNGG 1 cut(s) 327
Bme18I GGWCC 1 cut(s) 215
BmgT120I GGNCC 5 cut(s) 31, 32, 45, 46, 215
BmiI GGNNCC 2 cut(s) 33, 47
BmrFI CCNGG 1 cut(s) 327
BmsI GCATC 1 cut(s) 234
BpmI CTGGAG 1 cut(s) 401
BpuMI CCSGG 1 cut(s) 327
BsaAI YACGTR 3 cut(s) 14, 72, 104
BsaHI GRCGYC 2 cut(s) 284, 318
BsaI GGTCTC 1 cut(s) 91
BsaJI CCNNGG 2 cut(s) 121, 460
BsaWI WCCGGW 1 cut(s) 365
Bsc4I CCNNNNNNNGG 1 cut(s) 379
BseAI TCCGGA 1 cut(s) 365
BseDI CCNNGG 2 cut(s) 121, 460
BseGI GGATG 1 cut(s) 429
BseLI CCNNNNNNNGG 1 cut(s) 379
BseMII CTCAG 1 cut(s) 96
BseSI GKGCMC 2 cut(s) 35, 49
BseXI GCAGC 2 cut(s) 484, 493
BsgI GTGCAG 1 cut(s) 492
Bsh1285I CGRYCG 1 cut(s) 496
BshFI GGCC 4 cut(s) 33, 47, 299, 386
BsiEI CGRYCG 1 cut(s) 496
BsiHKAI GWGCWC 1 cut(s) 393
BsiSI CCGG 2 cut(s) 326, 366
BslI CCNNNNNNNGG 1 cut(s) 379
BsmAI GTCTC 3 cut(s) 91, 204, 515
BsmBI CGTCTC 1 cut(s) 204
BsmI GAATGC 1 cut(s) 243
BsnI GGCC 4 cut(s) 33, 47, 299, 386
Bso31I GGTCTC 1 cut(s) 91
Bsp120I GGGCCC 2 cut(s) 31, 45
Bsp1286I GDGCHC 4 cut(s) 35, 49, 158, 393
Bsp13I TCCGGA 1 cut(s) 365
Bsp143I GATC 2 cut(s) 6, 493
BspACI CCGC 3 cut(s) 315, 394, 397
BspANI GGCC 4 cut(s) 33, 47, 299, 386
BspCNI CTCAG 1 cut(s) 95
BspEI TCCGGA 1 cut(s) 365
BspLI GGNNCC 2 cut(s) 33, 47
BspTNI GGTCTC 1 cut(s) 91
BssECI CCNNGG 2 cut(s) 121, 460
BssMI GATC 2 cut(s) 6, 493
BssNI GRCGYC 2 cut(s) 284, 318
BssT1I CCWWGG 1 cut(s) 460
Bst4CI ACNGT 1 cut(s) 310
BstACI GRCGYC 2 cut(s) 284, 318
BstBAI YACGTR 3 cut(s) 14, 72, 104
BstDEI CTNAG 2 cut(s) 82, 108
BstDSI CCRYGG 1 cut(s) 121
BstF5I GGATG 1 cut(s) 429
BstKTI GATC 2 cut(s) 9, 496
BstMAI GTCTC 3 cut(s) 91, 204, 515
BstMBI GATC 2 cut(s) 6, 493
BstMCI CGRYCG 1 cut(s) 496
BstNSI RCATGY 1 cut(s) 138
BstSCI CCNGG 1 cut(s) 325
BstSLI GKGCMC 2 cut(s) 35, 49
BstSNI TACGTA 1 cut(s) 14
BstV1I GCAGC 2 cut(s) 484, 493
BstXI CCANNNNNNTGG 1 cut(s) 42
BsuRI GGCC 4 cut(s) 33, 47, 299, 386
BtgI CCRYGG 1 cut(s) 121
BtsCI GGATG 1 cut(s) 429
BtsIMutI CAGTG 2 cut(s) 509, 576
Cfr13I GGNCC 5 cut(s) 31, 32, 45, 46, 215
CpoI CGGWCCG 1 cut(s) 215
CseI GACGC 2 cut(s) 292, 307
CspI CGGWCCG 1 cut(s) 215
CviAII CATG 1 cut(s) 135
DdeI CTNAG 2 cut(s) 82, 108
DpnI GATC 2 cut(s) 8, 495
DpnII GATC 2 cut(s) 6, 493
DrdI GACNNNNNNGTC 1 cut(s) 83
DseDI GACNNNNNNGTC 1 cut(s) 83
EciI GGCGGA 1 cut(s) 383
Ecl136II GAGCTC 1 cut(s) 391
Eco105I TACGTA 1 cut(s) 14
Eco130I CCWWGG 1 cut(s) 460
Eco24I GRGCYC 4 cut(s) 35, 49, 158, 393
Eco31I GGTCTC 1 cut(s) 91
Eco47I GGWCC 1 cut(s) 215
Eco53kI GAGCTC 1 cut(s) 391
Eco57I CTGAAG 1 cut(s) 211
Eco72I CACGTG 2 cut(s) 72, 104
EcoICRI GAGCTC 1 cut(s) 391
EcoO109I RGGNCCY 1 cut(s) 46
EcoRI GAATTC 2 cut(s) 455, 549
EcoT14I CCWWGG 1 cut(s) 460
EcoT38I GRGCYC 4 cut(s) 35, 49, 158, 393
ErhI CCWWGG 1 cut(s) 460
Esp3I CGTCTC 1 cut(s) 204
FaeI CATG 1 cut(s) 138
FaiI YATR 5 cut(s) 136, 350, 501, 541, 543
FatI CATG 1 cut(s) 134
FauNDI CATATG 1 cut(s) 541
Fnu4HI GCNGC 4 cut(s) 316, 397, 473, 507
FokI GGATG 1 cut(s) 436
FriOI GRGCYC 4 cut(s) 35, 49, 158, 393
Fsp4HI GCNGC 4 cut(s) 316, 397, 473, 507
FspBI CTAG 1 cut(s) 531
GluI GCNGC 4 cut(s) 316, 397, 473, 507
GsuI CTGGAG 1 cut(s) 401
HaeIII GGCC 4 cut(s) 33, 47, 299, 386
HapII CCGG 2 cut(s) 326, 366
HgaI GACGC 2 cut(s) 292, 307
Hin1I GRCGYC 2 cut(s) 284, 318
Hin1II CATG 1 cut(s) 138
HinfI GANTC 4 cut(s) 278, 413, 535, 581
HpaII CCGG 2 cut(s) 326, 366
Hpy166II GTNNAC 2 cut(s) 121, 265
Hpy188I TCNGA 2 cut(s) 67, 564
Hpy188III TCNNGA 2 cut(s) 234, 366
Hpy8I GTNNAC 2 cut(s) 121, 265
Hpy99I CGWCG 3 cut(s) 223, 286, 323
HpyAV CCTTC 3 cut(s) 194, 298, 310
HpyCH4III ACNGT 1 cut(s) 310
HpyCH4IV ACGT 3 cut(s) 13, 71, 103
HpyCH4V TGCA 3 cut(s) 352, 472, 509
HpyF3I CTNAG 2 cut(s) 82, 108
HpySE526I ACGT 3 cut(s) 13, 71, 103
Hsp92I GRCGYC 2 cut(s) 284, 318
Hsp92II CATG 1 cut(s) 138
Kpn2I TCCGGA 1 cut(s) 365
Kzo9I GATC 2 cut(s) 6, 493
LmnI GCTCC 1 cut(s) 396
LpnPI CCDG 8 cut(s) 101, 180, 208, 214, 339, 365, 379, 570
Lsp1109I GCAGC 2 cut(s) 484, 493
LweI GCATC 1 cut(s) 234
MaeI CTAG 1 cut(s) 531
MaeII ACGT 3 cut(s) 13, 71, 103
MaeIII GTNAC 3 cut(s) 74, 275, 338
MalI GATC 2 cut(s) 8, 495
MboI GATC 2 cut(s) 6, 493
MboII GAAGA 3 cut(s) 138, 187, 283
MhlI GDGCHC 4 cut(s) 35, 49, 158, 393
MluCI AATT 4 cut(s) 159, 455, 549, 558
MlyI GAGTC 1 cut(s) 272
MnlI CCTC 4 cut(s) 91, 246, 376, 414
MroI TCCGGA 1 cut(s) 365
MspA1I CMGCKG 1 cut(s) 379
MspI CCGG 2 cut(s) 326, 366
MspR9I CCNGG 1 cut(s) 327
Mva1269I GAATGC 1 cut(s) 243
NciI CCSGG 1 cut(s) 327
NdeI CATATG 1 cut(s) 541
NdeII GATC 2 cut(s) 6, 493
NlaIII CATG 1 cut(s) 138
NlaIV GGNNCC 2 cut(s) 33, 47
NmeAIII GCCGAG 1 cut(s) 412
NmuCI GTSAC 2 cut(s) 74, 275
NspI RCATGY 1 cut(s) 138
PciI ACATGT 1 cut(s) 134
PcsI WCGNNNNNNNCGW 3 cut(s) 239, 327, 408
PctI GAATGC 1 cut(s) 243
PfeI GAWTC 3 cut(s) 413, 535, 581
PflMI CCANNNNNTGG 1 cut(s) 379
PkrI GCNGC 4 cut(s) 317, 398, 474, 508
Ple19I CGATCG 1 cut(s) 496
PleI GAGTC 1 cut(s) 272
PmaCI CACGTG 2 cut(s) 72, 104
PmlI CACGTG 2 cut(s) 72, 104
PpsI GAGTC 1 cut(s) 272
Ppu21I YACGTR 3 cut(s) 14, 72, 104
PscI ACATGT 1 cut(s) 134
Psp124BI GAGCTC 1 cut(s) 393
PspCI CACGTG 2 cut(s) 72, 104
PspN4I GGNNCC 2 cut(s) 33, 47
PspOMI GGGCCC 2 cut(s) 31, 45
PspPI GGNCC 5 cut(s) 31, 32, 45, 46, 215
PvuI CGATCG 1 cut(s) 496
PvuII CAGCTG 1 cut(s) 379
Rsr2I CGGWCCG 1 cut(s) 215
RsrII CGGWCCG 1 cut(s) 215
SacI GAGCTC 1 cut(s) 393
SatI GCNGC 4 cut(s) 316, 397, 473, 507
Sau3AI GATC 2 cut(s) 6, 493
Sau96I GGNCC 5 cut(s) 31, 32, 45, 46, 215
SchI GAGTC 1 cut(s) 272
ScrFI CCNGG 1 cut(s) 327
SduI GDGCHC 4 cut(s) 35, 49, 158, 393
SfaNI GCATC 1 cut(s) 234
SinI GGWCC 1 cut(s) 215
SnaBI TACGTA 1 cut(s) 14
Sse9I AATT 4 cut(s) 159, 455, 549, 558
SsiI CCGC 3 cut(s) 315, 394, 397
SspMI CTAG 1 cut(s) 531
SstI GAGCTC 1 cut(s) 393
StyD4I CCNGG 1 cut(s) 325
StyI CCWWGG 1 cut(s) 460
TaaI ACNGT 1 cut(s) 310
TaiI ACGT 3 cut(s) 16, 74, 106
TaqI TCGA 2 cut(s) 233, 281
TaqII GACCGA 1 cut(s) 232
TasI AATT 4 cut(s) 159, 455, 549, 558
TauI GCSGC 2 cut(s) 318, 399
TfiI GAWTC 3 cut(s) 413, 535, 581
TscAI CASTG 2 cut(s) 516, 583
TseFI GTSAC 2 cut(s) 74, 275
TseI GCWGC 2 cut(s) 472, 506
Tsp45I GTSAC 2 cut(s) 74, 275
TspGWI ACGGA 2 cut(s) 138, 228
TspRI CASTG 2 cut(s) 516, 583
Van91I CCANNNNNTGG 1 cut(s) 379
VpaK11BI GGWCC 1 cut(s) 215
XapI RAATTY 2 cut(s) 455, 549
XceI RCATGY 1 cut(s) 138
XspI CTAG 1 cut(s) 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.