Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0009010

Physical Location & Seq

Genomic Coordinates Forward (+)

33170611 .. 33172145

Gene Structure 1535 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1235500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 972 bp

ATGGCAGTTGAAGCTCAGCACCACCACCATGCAGTTCTAATCCCATCGCAACTAATCCCCAACAGAGATTTCATCAAAACGAACCAAGGAAATCCAAATATCTACAGTACCCAGATGGATTCCGGCCTTCTTTTGTCGACAACAATGCCGGAAAATCTCCTTCCGATGTATCAGTCTCCTTTCTGTGATGCAAATAAGGCTTCCATGAACAAGGACGCGGCAGACAGTGGCCTCACATACAACAACATCTCCGCTCCTCGGAAGCGGCCGAGAGACCAATTAAACAATGACTTCGACAATTTCTCGGTCCCTCACAAGAACAAAATCTCCTCTTTTTTCGACCAAGACATTGCTTTTCAGATCCAACAACAGCAATCTGAGATCGACCGGTTCATTGCCCAACATTCGGAAAAAGTGAGATTCGAACTCGAGGAGCAAAGAAAGCAGCAATCGAGAATGTTAGTGTCGGCTATCAAGGAGAATATCGTGAAGAAACTGAAAGAGAAAGATGAGGAGATTCAGAGGGTGGGAAAGCTCAATTGGGTTCTCCAGGAACGGGTCAAGAGCCTATTCGTCGAGAATCAGATATGGAGGGACATGGCACAGACAAACGAAGCCGCAGCCAATTCCCTACGCACCAATTTAGAGCAAGTCCTGGCGCACGTTAGCGACGACCACCACGCTGGCGGCAGCGGTGCCACGGCTGCCGCTGTGGCGGATGACGCCCAATCGTGCTGCGGGAGCAACGACAACGGGCGGGATATTGATGCTGTGGACAGCGGAAATGTGGCTGTTCGCGGTGGTTGTGGTGGTGGAAGTAGGATGTGTAAGAGGTGTGGGGTGAGAGAGTCGAGGGTGTTGTTGCTGCCATGCAGGCATTTGTGTTTGTGTACAATGTGTGGGTCCACTGTGCTCAATTGCCCCGTATGCAACTCTGTCATGAATGCCAGTGTGCATGTTAATTTCTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009719 GO:0009725 GO:0009739 GO:0009892 GO:0009987 GO:0010033 GO:0010466 GO:0010605 GO:0010941 GO:0010951 GO:0016567 GO:0016740 GO:0019222 GO:0019538 GO:0019787 GO:0030162 GO:0031323 GO:0031324 GO:0032268 GO:0032269 GO:0032446 GO:0033993 GO:0036211 GO:0042221 GO:0042742 GO:0043067 GO:0043069 GO:0043086 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045861 GO:0048519 GO:0048523 GO:0050789 GO:0050790 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0051704 GO:0051707 GO:0052547 GO:0052548 GO:0060255 GO:0060548 GO:0065007 GO:0065009 GO:0070647 GO:0071704 GO:0080090 GO:0098542 GO:0140096 GO:1901564 GO:1901700 GO:2000116 GO:2000117

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-C3HC4_3

Protein Analysis

324

Amino Acids

35.87

Weight (kDa)

6.59

Isoelectric Point (pI)

46.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-C3HC4_3	PF13920	274 - 314	7.6e-09	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009010)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G19700 AT5G45100 AT5G45100
fragaria_vesca	FvH4_3g12030 FvH4_3g12030
malus_domestica	MD05G1255400.v1.1 MD10G1235500.v1.1
prunus_persica	Prupe.4G108500_v2.0.a1
pyrus_communis	pycom05g23380 pycom10g19700 pycom10g19710
rosa_chinensis	RchiOBHm_Chr5g0019721
rosa_laevigata	RLG00000032464
rosa_multiflora	Rmu_ssc0000062.1_g000019
rosa_roxburghii	Rroxscaffold_1G00058390
rosa_rugosa	Rorug05G0053500
rosa_samantha	Rh5AG143800 Rh5BG142700 Rh5CG154100 Rh5CG154200 Rh5DG142800
rosa_wichuraiana	Rw5G012730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	695
AccBSI	CCGCTC	1 cut(s)	254
AccI	GTMKAC	1 cut(s)	137
AccII	CGCG	2 cut(s)	218, 798
AclWI	GGATC	1 cut(s)	355
AcoI	YGGCCR	1 cut(s)	266
AcyI	GRCGYC	1 cut(s)	723
AfaI	GTAC	2 cut(s)	109, 892
AfiI	CCNNNNNNNGG	3 cut(s)	258, 406, 556
AgeI	ACCGGT	1 cut(s)	387
AgsI	TTSAA	1 cut(s)	11
AjnI	CCWGG	2 cut(s)	549, 654
AjuI	GAANNNNNNNTTGG	4 cut(s)	53, 85, 523, 555
AloI	GAACNNNNNNTCC	2 cut(s)	311, 343
AluBI	AGCT	2 cut(s)	14, 535
AluI	AGCT	2 cut(s)	14, 535
Alw21I	GWGCWC	1 cut(s)	915
Alw26I	GTCTC	2 cut(s)	180, 267
AlwI	GGATC	1 cut(s)	355
Ama87I	CYCGRG	1 cut(s)	428
AoxI	GGCC	3 cut(s)	124, 229, 266
ApeKI	GCWGC	6 cut(s)	445, 620, 690, 704, 735, 865
AsiGI	ACCGGT	1 cut(s)	387
AspLEI	GCGC	1 cut(s)	661
AspS9I	GGNCC	2 cut(s)	307, 903
AsuHPI	GGTGA	1 cut(s)	853
AsuII	TTCGAA	1 cut(s)	423
AvaI	CYCGRG	1 cut(s)	428
AvaII	GGWCC	2 cut(s)	307, 903
BanI	GGYRCC	1 cut(s)	695
Bbv12I	GWGCWC	1 cut(s)	915
BbvI	GCAGC	6 cut(s)	457, 632, 691, 702, 722, 852
BccI	CCATC	2 cut(s)	52, 109
BceAI	ACGGC	1 cut(s)	717
BcgI	CGANNNNNNTGC	2 cut(s)	127, 161
BciT130I	CCWGG	2 cut(s)	551, 656
BcoDI	GTCTC	2 cut(s)	180, 267
BfmI	CTRYAG	1 cut(s)	103
BglI	GCCNNNNNGGC	2 cut(s)	713, 874
BlpI	GCTNAGC	1 cut(s)	15
Bme1390I	CCNGG	2 cut(s)	551, 656
Bme18I	GGWCC	2 cut(s)	307, 903
BmeT110I	CYCGRG	1 cut(s)	428
BmgT120I	GGNCC	2 cut(s)	307, 903
BmiI	GGNNCC	3 cut(s)	309, 697, 904
BmrFI	CCNGG	2 cut(s)	551, 656
BmsI	GCATC	2 cut(s)	178, 757
BpmI	CTGGAG	1 cut(s)	533
Bpu1102I	GCTNAGC	1 cut(s)	15
Bpu14I	TTCGAA	1 cut(s)	423
BsaHI	GRCGYC	1 cut(s)	723
BsaI	GGTCTC	1 cut(s)	267
BsaJI	CCNNGG	3 cut(s)	85, 257, 699
BsaWI	WCCGGW	1 cut(s)	387
BsaXI	ACNNNNNCTCC	4 cut(s)	233, 263, 311, 341
Bsc4I	CCNNNNNNNGG	3 cut(s)	258, 406, 556
Bse118I	RCCGGY	1 cut(s)	387
Bse1I	ACTGG	1 cut(s)	948
Bse3DI	GCAATG	2 cut(s)	348, 393
BseBI	CCWGG	2 cut(s)	551, 656
BseDI	CCNNGG	3 cut(s)	85, 257, 699
BseGI	GGATG	2 cut(s)	724, 828
BseLI	CCNNNNNNNGG	3 cut(s)	258, 406, 556
BseMI	GCAATG	2 cut(s)	348, 393
BseMII	CTCAG	2 cut(s)	29, 369
BseNI	ACTGG	1 cut(s)	948
BseRI	GAGGAG	4 cut(s)	246, 319, 446, 527
BseX3I	CGGCCG	1 cut(s)	266
BseXI	GCAGC	6 cut(s)	457, 632, 691, 702, 722, 852
Bsh1236I	CGCG	2 cut(s)	218, 798
Bsh1285I	CGRYCG	2 cut(s)	269, 388
BshFI	GGCC	3 cut(s)	126, 231, 268
BshNI	GGYRCC	1 cut(s)	695
BshTI	ACCGGT	1 cut(s)	387
BsiEI	CGRYCG	2 cut(s)	269, 388
BsiHKAI	GWGCWC	1 cut(s)	915
BsiHKCI	CYCGRG	1 cut(s)	428
BsiSI	CCGG	3 cut(s)	123, 149, 388
BslFI	GGGAC	2 cut(s)	293, 608
BslI	CCNNNNNNNGG	3 cut(s)	258, 406, 556
BsmAI	GTCTC	2 cut(s)	180, 267
BsmFI	GGGAC	2 cut(s)	293, 608
BsmI	GAATGC	1 cut(s)	949
BsnI	GGCC	3 cut(s)	126, 231, 268
Bso31I	GGTCTC	1 cut(s)	267
BsoBI	CYCGRG	1 cut(s)	428
Bsp119I	TTCGAA	1 cut(s)	423
Bsp1286I	GDGCHC	1 cut(s)	915
Bsp1407I	TGTACA	1 cut(s)	890
Bsp143I	GATC	2 cut(s)	360, 381
Bsp1720I	GCTNAGC	1 cut(s)	15
BspANI	GGCC	3 cut(s)	126, 231, 268
BspCNI	CTCAG	2 cut(s)	28, 370
BspFNI	CGCG	2 cut(s)	218, 798
BspHI	TCATGA	1 cut(s)	939
BspLI	GGNNCC	3 cut(s)	309, 697, 904
BspPI	GGATC	1 cut(s)	355
BspT104I	TTCGAA	1 cut(s)	423
BspT107I	GGYRCC	1 cut(s)	695
BspTNI	GGTCTC	1 cut(s)	267
BsrBI	CCGCTC	1 cut(s)	254
BsrDI	GCAATG	2 cut(s)	348, 393
BsrFI	RCCGGY	1 cut(s)	387
BsrGI	TGTACA	1 cut(s)	890
BsrI	ACTGG	1 cut(s)	948
BssAI	RCCGGY	1 cut(s)	387
BssECI	CCNNGG	3 cut(s)	85, 257, 699
BssMI	GATC	2 cut(s)	360, 381
BssNI	GRCGYC	1 cut(s)	723
BssT1I	CCWWGG	1 cut(s)	85
Bst2UI	CCWGG	2 cut(s)	551, 656
Bst4CI	ACNGT	3 cut(s)	107, 227, 910
BstACI	GRCGYC	1 cut(s)	723
BstAUI	TGTACA	1 cut(s)	890
BstBI	TTCGAA	1 cut(s)	423
BstC8I	GCNNGC	2 cut(s)	685, 875
BstDEI	CTNAG	3 cut(s)	15, 378, 969
BstDSI	CCRYGG	1 cut(s)	699
BstF5I	GGATG	2 cut(s)	724, 828
BstFNI	CGCG	2 cut(s)	218, 798
BstHHI	GCGC	1 cut(s)	661
BstKTI	GATC	2 cut(s)	363, 384
BstMAI	GTCTC	2 cut(s)	180, 267
BstMBI	GATC	2 cut(s)	360, 381
BstMCI	CGRYCG	2 cut(s)	269, 388
BstMWI	GCNNNNNNNGC	9 cut(s)	11, 197, 442, 704, 713, 722, 741, 874, 927
BstNI	CCWGG	2 cut(s)	551, 656
BstNSI	RCATGY	1 cut(s)	959
BstSCI	CCNGG	2 cut(s)	549, 654
BstSFI	CTRYAG	1 cut(s)	103
BstUI	CGCG	2 cut(s)	218, 798
BstV1I	GCAGC	6 cut(s)	457, 632, 691, 702, 722, 852
BstX2I	RGATCY	1 cut(s)	360
BstXI	CCANNNNNNTGG	1 cut(s)	683
BstYI	RGATCY	1 cut(s)	360
BstZI	CGGCCG	1 cut(s)	266
BsuRI	GGCC	3 cut(s)	126, 231, 268
BtgI	CCRYGG	1 cut(s)	699
BtgZI	GCGATG	1 cut(s)	30
BtsCI	GGATG	2 cut(s)	724, 828
BtsIMutI	CAGTG	3 cut(s)	232, 906, 955
Cac8I	GCNNGC	2 cut(s)	685, 875
CciI	TCATGA	1 cut(s)	939
CfoI	GCGC	1 cut(s)	661
Cfr10I	RCCGGY	1 cut(s)	387
Cfr13I	GGNCC	2 cut(s)	307, 903
CseI	GACGC	2 cut(s)	224, 731
Csp6I	GTAC	2 cut(s)	108, 891
CspAI	ACCGGT	1 cut(s)	387
CviAII	CATG	6 cut(s)	29, 205, 598, 870, 940, 956
CviQI	GTAC	2 cut(s)	108, 891
DdeI	CTNAG	3 cut(s)	15, 378, 969
DpnI	GATC	2 cut(s)	362, 383
DpnII	GATC	2 cut(s)	360, 381
EaeI	YGGCCR	1 cut(s)	266
EagI	CGGCCG	1 cut(s)	266
EciI	GGCGGA	1 cut(s)	731
EclXI	CGGCCG	1 cut(s)	266
Eco130I	CCWWGG	1 cut(s)	85
Eco31I	GGTCTC	1 cut(s)	267
Eco47I	GGWCC	2 cut(s)	307, 903
Eco52I	CGGCCG	1 cut(s)	266
Eco88I	CYCGRG	1 cut(s)	428
EcoRII	CCWGG	2 cut(s)	549, 654
EcoT14I	CCWWGG	1 cut(s)	85
ErhI	CCWWGG	1 cut(s)	85
FaeI	CATG	6 cut(s)	32, 208, 601, 873, 943, 959
FaiI	YATR	9 cut(s)	30, 206, 238, 589, 599, 871, 928, 941, 957
FaqI	GGGAC	2 cut(s)	293, 608
FatI	CATG	6 cut(s)	28, 204, 597, 869, 939, 955
FauI	CCCGC	2 cut(s)	731, 750
FblI	GTMKAC	1 cut(s)	137
FokI	GGATG	2 cut(s)	731, 835
GlaI	GCGC	1 cut(s)	660
GsuI	CTGGAG	1 cut(s)	533
HaeIII	GGCC	3 cut(s)	126, 231, 268
HapII	CCGG	3 cut(s)	123, 149, 388
HgaI	GACGC	2 cut(s)	224, 731
HhaI	GCGC	1 cut(s)	661
Hin1I	GRCGYC	1 cut(s)	723
Hin1II	CATG	6 cut(s)	32, 208, 601, 873, 943, 959
Hin6I	GCGC	1 cut(s)	659
HinP1I	GCGC	1 cut(s)	659
HincII	GTYRAC	1 cut(s)	138
HindII	GTYRAC	1 cut(s)	138
HinfI	GANTC	5 cut(s)	119, 420, 517, 580, 848
HpaII	CCGG	3 cut(s)	123, 149, 388
HphI	GGTGA	1 cut(s)	853
Hpy166II	GTNNAC	4 cut(s)	138, 775, 891, 906
Hpy188I	TCNGA	7 cut(s)	165, 261, 360, 379, 409, 522, 585
Hpy188III	TCNNGA	5 cut(s)	453, 487, 562, 577, 940
Hpy8I	GTNNAC	4 cut(s)	138, 775, 891, 906
Hpy99I	CGWCG	2 cut(s)	578, 674
HpyAV	CCTTC	2 cut(s)	137, 170
HpyCH4III	ACNGT	3 cut(s)	107, 227, 910
HpyCH4IV	ACGT	1 cut(s)	663
HpyCH4V	TGCA	5 cut(s)	32, 191, 873, 930, 955
HpyF10VI	GCNNNNNNNGC	9 cut(s)	11, 197, 442, 704, 713, 722, 741, 874, 927
HpyF3I	CTNAG	3 cut(s)	15, 378, 969
HpySE526I	ACGT	1 cut(s)	663
Hsp92I	GRCGYC	1 cut(s)	723
Hsp92II	CATG	6 cut(s)	32, 208, 601, 873, 943, 959
HspAI	GCGC	1 cut(s)	659
Kzo9I	GATC	2 cut(s)	360, 381
LmnI	GCTCC	3 cut(s)	259, 433, 741
Lsp1109I	GCAGC	6 cut(s)	457, 632, 691, 702, 722, 852
LweI	GCATC	2 cut(s)	178, 757
MaeII	ACGT	1 cut(s)	663
MalI	GATC	2 cut(s)	362, 383
MbiI	CCGCTC	1 cut(s)	254
MboI	GATC	2 cut(s)	360, 381
MboII	GAAGA	1 cut(s)	502
MfeI	CAATTG	2 cut(s)	538, 916
MflI	RGATCY	1 cut(s)	360
MhlI	GDGCHC	1 cut(s)	915
MluCI	AATT	7 cut(s)	278, 298, 538, 625, 640, 916, 961
MlyI	GAGTC	1 cut(s)	857
MmeI	TCCRAC	1 cut(s)	388
MseI	TTAA	2 cut(s)	281, 960
MslI	CAYNNNNRTG	1 cut(s)	27
MspA1I	CMGCKG	3 cut(s)	693, 710, 780
MspI	CCGG	3 cut(s)	123, 149, 388
MspR9I	CCNGG	2 cut(s)	551, 656
MunI	CAATTG	2 cut(s)	538, 916
Mva1269I	GAATGC	1 cut(s)	949
MvaI	CCWGG	2 cut(s)	551, 656
MvnI	CGCG	2 cut(s)	218, 798
MwoI	GCNNNNNNNGC	9 cut(s)	11, 197, 442, 704, 713, 722, 741, 874, 927
NdeII	GATC	2 cut(s)	360, 381
NlaIII	CATG	6 cut(s)	32, 208, 601, 873, 943, 959
NlaIV	GGNNCC	3 cut(s)	309, 697, 904
NmeAIII	GCCGAG	1 cut(s)	294
NspI	RCATGY	1 cut(s)	959
NspV	TTCGAA	1 cut(s)	423
PaeR7I	CTCGAG	1 cut(s)	428
PagI	TCATGA	1 cut(s)	939
PcsI	WCGNNNNNNNCGW	1 cut(s)	669
PctI	GAATGC	1 cut(s)	949
PfeI	GAWTC	4 cut(s)	119, 420, 517, 580
PfoI	TCCNGGA	1 cut(s)	549
PinAI	ACCGGT	1 cut(s)	387
PleI	GAGTC	1 cut(s)	856
PpsI	GAGTC	1 cut(s)	856
Psp6I	CCWGG	2 cut(s)	549, 654
PspGI	CCWGG	2 cut(s)	549, 654
PspN4I	GGNNCC	3 cut(s)	309, 697, 904
PspPI	GGNCC	2 cut(s)	307, 903
PspXI	VCTCGAGB	1 cut(s)	428
PsuI	RGATCY	1 cut(s)	360
RsaI	GTAC	2 cut(s)	109, 892
RsaNI	GTAC	2 cut(s)	108, 891
RseI	CAYNNNNRTG	1 cut(s)	27
SalI	GTCGAC	1 cut(s)	136
SaqAI	TTAA	2 cut(s)	281, 960
Sau3AI	GATC	2 cut(s)	360, 381
Sau96I	GGNCC	2 cut(s)	307, 903
SchI	GAGTC	1 cut(s)	857
ScrFI	CCNGG	2 cut(s)	551, 656
SduI	GDGCHC	1 cut(s)	915
SetI	ASST	4 cut(s)	16, 537, 666, 836
SfaNI	GCATC	2 cut(s)	178, 757
SfcI	CTRYAG	1 cut(s)	103
Sfr274I	CTCGAG	1 cut(s)	428
SfuI	TTCGAA	1 cut(s)	423
SinI	GGWCC	2 cut(s)	307, 903
SlaI	CTCGAG	1 cut(s)	428
SmiMI	CAYNNNNRTG	1 cut(s)	27
SmlI	CTYRAG	1 cut(s)	428
SmoI	CTYRAG	1 cut(s)	428
Sse9I	AATT	7 cut(s)	278, 298, 538, 625, 640, 916, 961
StyD4I	CCNGG	2 cut(s)	549, 654
StyI	CCWWGG	1 cut(s)	85
TaaI	ACNGT	3 cut(s)	107, 227, 910
TaiI	ACGT	1 cut(s)	666
TaqI	TCGA	9 cut(s)	137, 294, 339, 384, 423, 429, 452, 576, 851
TaqII	GACCGA	1 cut(s)	295
TasI	AATT	7 cut(s)	278, 298, 538, 625, 640, 916, 961
TatI	WGTACW	1 cut(s)	890
TauI	GCSGC	5 cut(s)	221, 268, 620, 690, 710
TfiI	GAWTC	4 cut(s)	119, 420, 517, 580
Tru1I	TTAA	2 cut(s)	281, 960
Tru9I	TTAA	2 cut(s)	281, 960
TscAI	CASTG	3 cut(s)	232, 913, 955
TseI	GCWGC	6 cut(s)	445, 620, 690, 704, 735, 865
TspDTI	ATGAA	4 cut(s)	61, 221, 382, 956
TspRI	CASTG	3 cut(s)	232, 913, 955
VpaK11BI	GGWCC	2 cut(s)	307, 903
XceI	RCATGY	1 cut(s)	959
XhoI	CTCGAG	1 cut(s)	428
XmiI	GTMKAC	1 cut(s)	137

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.