Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0016785

Physical Location & Seq

Genomic Coordinates Forward (+)

33456623 .. 33461697

Gene Structure 5075 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1238400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3768 bp

ATGGCAGGATCGTCTGGAAGAACAAAGAGGAAGATAAGGTGGAGCAAACTGTATTCTTTTGCATGCTTCAGACCTGTCACAACTGAGAATGATCCAAGCCAGCAGCTCCTTGGCCAGCCGGGGTTTTCGCGGGTGGTTTTCTGCAACGAGCCTGAGCTGCACAAGGCCAAGCCTTACAAGTACCCCAAGAACTATGTCTCCACAACCAAGTACAATATCATGACTTTTCTTCCAAAAGCGCTTTTCGAGCAGTTCCGAAGAGTTGCCAATCTGTATTTTCTGTTGGCAGCTGTGTTGTCCGTCACTTATTTGGCTCCTTTTCAACCAATAAGTTTGATTGCTCCTTTGGTCTTTGTGGTGGGGGTTAGCATGATCAAGGAGGCAGTGGAGGACTGGCAAAGATTCTTGCAGGATTTGAATGTGAACTCAAGAACTGTAAAGGTTCACGTTGGAGACGGCGAATTTATTGAAAAACCATGGCAAGGACTGTGTGTAGGAGATGTTGTGAAGGTTAACAAGAATGAATATTTTCCTAGTGATCTCCTCTTACTATCTTCCAGCTATGAGGATGGCATATGTTATGTAGAGACCATGAACCTTGATGGAGAAACTAATCTCAAGGTTAAACGATGCTCGGAAGCCACACTTGGCCTTGTCAATGATCAATCATTTGTTTTATTCAGGGCTACCGTCCGCTGTGAGGACCCGAATCCTCATCTCTACACCTTTGTAGGGAACTTAGAGTTGAAAAATGCATCATTTCCCTTGTGTCCTGCCTCACTTCTCCTAAGAGATTCAAAGCTTCGGAACACTGATTATATTTATGGGGTTGTGATTTTTAGTGGACCAGACACAAAAGCTGTGAGGAACTCAACAAGATCACCATCTAAGCGCAGTCGGATTGAAAGAAAGATGGATCTTGTCATCTACCTTCTCTTTTCGATGCTTCTGTTGATTTCACTGGTCACTTTGATTGGTTTTGCAGAGTTTTTGAAAACGGAGATGATTAAGTGGTGGTATCTTTCTCTAGATGATAAAGATCCATTTTTTCAACCATCAAAGCCTGAGGTATCTGGTTTCCTACAGTTCATAAGGGCTCTTATTTTGTATGGTTACTTGATCCCCATTTCTCTCTATGTTTCCATTGAGGTTGTCAAAGTTTTACAAGCCATGCTCATTAACAAGGACATCAAATTGTATGATGAAGTTACATGTAAATCTGTTCAAACGCGAACATCAAATTTAAATGAAGAGCTTGGTCAGGTGGAGATGATTCTGTCTGATAAAACGGGGACTTTGACGTGTAACCAGATGGAATTCAGGAAATGCTCTATTGCTGGAATTTCGTATGGGGGTGATGTAAATGAAATTGATCGTGCAGCATCTCAACGGATGAATGTTGATGTTGAGTCATATTGTTTTAGTATTGATGAGTTTGAAACAGCAAGCCAAAGCTGCGAAATGTTTGAGTTCTCAGTTGGTGACATTAGCACTGAAAGGGCTATTCTAGGTGGACAAAGACATGAGCAGAATTATTCAAGTGCAGAAAACTCGAGAATCTCGAACGTGGAGGAAGAATCTGCCATCAAGGGTTTCAACTTTAGGGATGGCAGGCTTATGAACAAAAAGTGGATGTACAGATCCAACTTATCCGATGTAACAATGTTCTTTCGAGTCATGGCTCTATGTCACACAGGCATACCTGTTGAGGAGGATGAGCTAACTCATAAGCTAAAGTATGAGGCAGAGTCACCGGAAGAAGTATCTTTTCTAGTTGCTGCTCAAGAGTTTGGATTTCAATTTTTTCGAAGAAGTCAGTCCTTTATGTTTCTTAAGGAATTTGATCCTTCCACGGGAAAGGAGGTTGAGAGGAAGTACAAACTTTTGAACCTTTTAGAGTTCTGTAGCACTCGGAAAAGGATGTCTGTTATCATAAGCGACGAAGATGGTCAGATCATTCTACTCTGCAAAGGTGCAGACAACATTATCTTCGATAGGCTTGCAGAAAATGGAAGGGCATACCAACAGGCAACAACCCTACACCTTTCCAATTATGCAGAAGATGGATTCCGCACTCTGGCATTTGCTTACCGGAAACTTGAGGCTGCTGAGTATGAACAATGGAACTCAATATTCAAGGTGGCCAAGACAACAATAGGTCCCGAAAGGGAAGAAGTGCTAGAGAAAGCATCTGAAATGATCGAAAAGGATTTGATTTTATTAGGGGTTGCTGCTGTTGAAGACAAGCTACAAAAGGGGGTTCCAGAATGCATAGATAAGCTTGCCCAAGCCGGGATAAAAATATGGCTGCTCACCGGTGACAAGAAGGAAACTGCCATAAATATAGGATTTGCTTGCAGCTTGCTCCGGCAGGATATGAAGCAATTTCATCTGAGCTTGGGTAGAGAAACTGCAACTATTCCAACTAATAACCAACTGAAGGCGATGAAAGATGACATTTTAAACCAACTCGAAAGTTTTCACAAACTGAAGTCTGAAGAAGGCAATGATAATGCACCCTTGGCTTTGGTAGTAGATGGAAAAGCTCTTGAAATTGCTCTCAGAAGTGATGTGAAGGACCAGTTCTTACCATTGGCTGTTAATTGCGCTTCTGTCATATGCTGCCGCGTCTCTCCAAAACAGAAGGCTTTGATTACGCGATTGGTAAAGGAACATACTGGGAAAACAACCTTGGCAATTGGAGATGGGGCAAATGATGTTGGCATGATTCAAGAAGCTGATATTGGAGTAGGAATCAGTGGCATGGAAGGAATGCAGGCAGTCATGGCCAGTGACATAGCATTGCCTCAATTCCGATTTTTGGGGAGATTACTTATAGTTCATGGGCGTTGGTGTTACAAAAGGATTTCAAAAATGGTTCTCTACTTCGTGTACAAGAACATTGCGTTGGGGCTCACGCTGTTCTACTATGAGTTATCCTCAAGATTTGCAGGGGAGGTCTTCTACGACGACTGGTATATGGCCTTGTTCAATGTGATTTTGACATCTTTACCTGTTATATCATTGGGAGTCCTTGAGCAGGACGTTTCATCAGAAGTGTGCCTCCAGTTTCCAGCTCTTTATCAACAAGGACAGAAGAACATATATTTCACGTGGTACCGGATTATTGGTTGGATAGTAAACGGCGTGGTTGCTTCTCTAGTCATATTCCTTGCAAACATATACACTTTCTCCCCTAATTCATTTCAGAAGAATGGCCAAGTTGCAGACATCACACACCTTGGTGCCATGACATACACCTGCATAATCTGGACCGTCAATTGCCAGATTGCGCTGATCATCGACCGGTTCACTTGGATCCAACATCTTTTCATATGGGGTAGCATCATGATCTGGTACGTGTTCTTGTTGGTATACGGTGCATTCCCTCCTGCCTACTCTCAAAGAGGATTTCGTGTCCTAATCGAAGCTCTTGGCCCTGCACCGTTGTATTGGACTGTCACATTATTCGTTGTGGTTGTTTCGCTGCTACCCTACTTCATTCACATCGTCATCCAACGGTCATTCTATCCGTTGGACGATCATGTGATTCAAGAAATGAAGCACTGCAGGAAAGATGTTGCAGTCAAGCAAATGTGGGAGAGGGAGAGAAGCAACTCCATAAAGATGACTCAGGTTGGGGTTTCGGCTCGGGTTGACGCGAGAATTCGGGTTCTGAAAGAACAGTTGCACCACAAGAAACAGTTGATATACAGATCAGTGACAAGCAGTCCAATATTTAGATCACTGACAAACAGCCCAGCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006900 GO:0006996 GO:0008150 GO:0009987 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031984 GO:0033036 GO:0034204 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045332 GO:0048193 GO:0048194 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase Hydrolase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1256

Amino Acids

142.85

Weight (kDa)

7.43

Isoelectric Point (pI)

40.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhoLip_ATPase_N	PF16209	46 - 112	1.4e-22	Phospholipid-translocating ATPase N-terminal
Cation_ATPase	PF13246	555 - 752	2.2e-19	P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C	PF16212	928 - 1178	1.7e-84	Phospholipid-translocating P-type ATPase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0016785)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g11830
malus_domestica	MD10G1238400.v1.1
prunus_persica	Prupe.4G105900_v2.0.a1
pyrus_communis	pycom10g19940
rosa_chinensis	RchiOBHm_Chr5g0019261
rosa_laevigata	RLG00000032432
rosa_multiflora	Rmu_co8517133.1_g000001
rosa_roxburghii	Rroxscaffold_1G00058740
rosa_rugosa	Rorug05G0049000
rosa_samantha	Rh5AG140100 Rh5BG139100 Rh5CG150500 Rh5DG139700
rosa_wichuraiana	Rw5G012460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	3269
Acc36I	ACCTGC	1 cut(s)	3269
Acc65I	GGTACC	1 cut(s)	3117
AccB1I	GGYRCC	2 cut(s)	3117, 3245
AccI	GTMKAC	1 cut(s)	3375
AccII	CGCG	5 cut(s)	130, 1231, 2628, 2659, 3662
AciI	CCGC	4 cut(s)	130, 694, 2071, 2626
AclWI	GGATC	9 cut(s)	16, 86, 924, 1034, 1114, 1635, 1838, 3313, 3326
AcoI	YGGCCR	4 cut(s)	112, 2142, 2787, 3217
AcsI	RAATTY	6 cut(s)	461, 1240, 1316, 1341, 1838, 3666
AcuI	CTGAAG	4 cut(s)	52, 2459, 2510, 2517
AcvI	CACGTG	1 cut(s)	3114
AfaI	GTAC	7 cut(s)	182, 212, 1637, 1877, 2894, 3119, 3359
AfeI	AGCGCT	1 cut(s)	240
AfiI	CCNNNNNNNGG	9 cut(s)	482, 700, 732, 1853, 2077, 2292, 2440, 2821, 3040
AflII	CTTAAG	1 cut(s)	1832
AflIII	ACRYGT	3 cut(s)	1211, 1301, 3360
AgeI	ACCGGT	2 cut(s)	2315, 3306
AhdI	GACNNNNNGTC	1 cut(s)	3729
AjiI	CACGTC	1 cut(s)	1302
AjuI	GAANNNNNNNTTGG	8 cut(s)	630, 662, 2675, 2707, 2727, 2759, 2891, 2923
AleI	CACNNNNGTG	1 cut(s)	3243
AloI	GAACNNNNNNTCC	2 cut(s)	182, 214
Alw26I	GTCTC	4 cut(s)	202, 447, 581, 2635
AlwI	GGATC	9 cut(s)	16, 86, 924, 1034, 1114, 1635, 1838, 3313, 3326
Ama87I	CYCGRG	2 cut(s)	1552, 3651
Aor51HI	AGCGCT	1 cut(s)	240
AoxI	GGCC	8 cut(s)	112, 165, 649, 2142, 2787, 2982, 3217, 3436
ApoI	RAATTY	6 cut(s)	461, 1240, 1316, 1341, 1838, 3666
AsiGI	ACCGGT	2 cut(s)	2315, 3306
Asp700I	GAANNNNTTC	2 cut(s)	528, 2478
Asp718I	GGTACC	1 cut(s)	3117
AspLEI	GCGC	4 cut(s)	241, 894, 2609, 3295
AspS9I	GGNCC	6 cut(s)	703, 845, 2159, 2578, 3273, 3437
AsuC2I	CCSGG	2 cut(s)	120, 2293
AsuHPI	GGTGA	6 cut(s)	873, 1367, 1493, 1743, 2305, 2330
AsuII	TTCGAA	1 cut(s)	1807
AvaI	CYCGRG	2 cut(s)	1552, 3651
AvaII	GGWCC	5 cut(s)	703, 845, 2159, 2578, 3273
AxyI	CCTNAGG	1 cut(s)	1065
BalI	TGGCCA	4 cut(s)	114, 2144, 2789, 3219
BamHI	GGATCC	1 cut(s)	3318
BanI	GGYRCC	2 cut(s)	3117, 3245
BanII	GRGCYC	2 cut(s)	1099, 2916
BarI	GAAGNNNNNNTAC	2 cut(s)	2232, 2264
BbrPI	CACGTG	1 cut(s)	3114
BbsI	GAAGAC	2 cut(s)	2247, 2953
BceAI	ACGGC	2 cut(s)	472, 3160
BcgI	CGANNNNNNTGC	4 cut(s)	1533, 1567, 1982, 2016
BclI	TGATCA	3 cut(s)	372, 661, 3297
BcnI	CCSGG	2 cut(s)	120, 2293
BcoDI	GTCTC	4 cut(s)	202, 447, 581, 2635
BfaI	CTAG	7 cut(s)	534, 1028, 1508, 1772, 2180, 3161, 3766
BfmI	CTRYAG	3 cut(s)	1082, 1903, 3568
BfoI	RGCGCY	1 cut(s)	242
BfrI	CTTAAG	1 cut(s)	1832
BfuAI	ACCTGC	1 cut(s)	3269
Bme1390I	CCNGG	2 cut(s)	120, 2293
Bme18I	GGWCC	5 cut(s)	703, 845, 2159, 2578, 3273
BmeRI	GACNNNNNGTC	1 cut(s)	3729
BmeT110I	CYCGRG	2 cut(s)	1552, 3651
BmgBI	CACGTC	1 cut(s)	1302
BmgT120I	GGNCC	6 cut(s)	703, 845, 2159, 2578, 3273, 3437
BmiI	GGNNCC	7 cut(s)	315, 705, 2161, 2262, 3119, 3247, 3320
BmrFI	CCNGG	2 cut(s)	120, 2293
BmrI	ACTGGG	1 cut(s)	2688
BmsI	GCATC	6 cut(s)	620, 764, 933, 1391, 2198, 3354
BmuI	ACTGGG	1 cut(s)	2688
BpiI	GAAGAC	2 cut(s)	2247, 2953
BplI	GAGNNNNNCTC	2 cut(s)	2924, 2956
BpmI	CTGGAG	1 cut(s)	3050
Bpu10I	CCTNAGC	1 cut(s)	153
Bpu14I	TTCGAA	1 cut(s)	1807
BpuEI	CTTGAG	6 cut(s)	412, 602, 1767, 2120, 2926, 3056
BpuMI	CCSGG	2 cut(s)	120, 2293
BsaAI	YACGTR	2 cut(s)	3114, 3361
BsaBI	GATNNNNATC	2 cut(s)	883, 1038
BsaI	GGTCTC	1 cut(s)	581
BsaJI	CCNNGG	7 cut(s)	109, 119, 476, 1851, 2520, 2691, 3241
BsaWI	WCCGGW	5 cut(s)	1753, 2091, 2315, 3120, 3306
BsaXI	ACNNNNNCTCC	6 cut(s)	182, 212, 489, 519, 3176, 3206
Bsc4I	CCNNNNNNNGG	9 cut(s)	482, 700, 732, 1853, 2077, 2292, 2440, 2821, 3040
Bse118I	RCCGGY	2 cut(s)	2315, 3306
Bse1I	ACTGG	7 cut(s)	398, 966, 2581, 2683, 2790, 2978, 3067
Bse21I	CCTNAGG	1 cut(s)	1065
Bse3DI	GCAATG	3 cut(s)	2512, 2801, 2901
Bse8I	GATNNNNATC	2 cut(s)	883, 1038
BseDI	CCNNGG	7 cut(s)	109, 119, 476, 1851, 2520, 2691, 3241
BseGI	GGATG	7 cut(s)	574, 1398, 1612, 1638, 1720, 1926, 3513
BseJI	GATNNNNATC	2 cut(s)	883, 1038
BseLI	CCNNNNNNNGG	9 cut(s)	482, 700, 732, 1853, 2077, 2292, 2440, 2821, 3040
BseMI	GCAATG	3 cut(s)	2512, 2801, 2901
BseMII	CTCAG	8 cut(s)	75, 144, 1056, 1488, 2100, 2384, 2575, 3647
BseNI	ACTGG	7 cut(s)	398, 966, 2581, 2683, 2790, 2978, 3067
BseRI	GAGGAG	2 cut(s)	533, 1724
BseYI	CCCAGC	1 cut(s)	3760
BsgI	GTGCAG	5 cut(s)	143, 1398, 1563, 1995, 3426
Bsh1236I	CGCG	5 cut(s)	130, 1231, 2628, 2659, 3662
Bsh1285I	CGRYCG	1 cut(s)	3307
BshFI	GGCC	8 cut(s)	114, 167, 651, 2144, 2789, 2984, 3219, 3438
BshNI	GGYRCC	2 cut(s)	3117, 3245
BshTI	ACCGGT	2 cut(s)	2315, 3306
BsiEI	CGRYCG	1 cut(s)	3307
BsiHKCI	CYCGRG	2 cut(s)	1552, 3651
BsiSI	CCGG	8 cut(s)	119, 1754, 2092, 2292, 2316, 2368, 3121, 3307
BslFI	GGGAC	2 cut(s)	1306, 2145
BslI	CCNNNNNNNGG	9 cut(s)	482, 700, 732, 1853, 2077, 2292, 2440, 2821, 3040
BsmAI	GTCTC	4 cut(s)	202, 447, 581, 2635
BsmBI	CGTCTC	2 cut(s)	447, 2635
BsmFI	GGGAC	2 cut(s)	1306, 2145
BsmI	GAATGC	3 cut(s)	2273, 2778, 3383
BsnI	GGCC	8 cut(s)	114, 167, 651, 2144, 2789, 2984, 3219, 3438
Bso31I	GGTCTC	1 cut(s)	581
BsoBI	CYCGRG	2 cut(s)	1552, 3651
Bsp119I	TTCGAA	1 cut(s)	1807
Bsp1286I	GDGCHC	2 cut(s)	1099, 2916
Bsp1407I	TGTACA	2 cut(s)	1635, 2892
Bsp19I	CCATGG	1 cut(s)	476
BspACI	CCGC	4 cut(s)	130, 694, 2071, 2626
BspANI	GGCC	8 cut(s)	114, 167, 651, 2144, 2789, 2984, 3219, 3438
BspCNI	CTCAG	8 cut(s)	76, 145, 1057, 1487, 2101, 2385, 2574, 3646
BspFNI	CGCG	5 cut(s)	130, 1231, 2628, 2659, 3662
BspHI	TCATGA	2 cut(s)	219, 3348
BspLI	GGNNCC	7 cut(s)	315, 705, 2161, 2262, 3119, 3247, 3320
BspMAI	CTGCAG	1 cut(s)	3572
BspMI	ACCTGC	1 cut(s)	3269
BspPI	GGATC	9 cut(s)	16, 86, 924, 1034, 1114, 1635, 1838, 3313, 3326
BspQI	GCTCTTC	1 cut(s)	1245
BspT104I	TTCGAA	1 cut(s)	1807
BspT107I	GGYRCC	2 cut(s)	3117, 3245
BspTI	CTTAAG	1 cut(s)	1832
BspTNI	GGTCTC	1 cut(s)	581
BsrDI	GCAATG	3 cut(s)	2512, 2801, 2901
BsrFI	RCCGGY	2 cut(s)	2315, 3306
BsrGI	TGTACA	2 cut(s)	1635, 2892
BsrI	ACTGG	7 cut(s)	398, 966, 2581, 2683, 2790, 2978, 3067
BssAI	RCCGGY	2 cut(s)	2315, 3306
BssECI	CCNNGG	7 cut(s)	109, 119, 476, 1851, 2520, 2691, 3241
BssNAI	GTATAC	1 cut(s)	3376
BssT1I	CCWWGG	5 cut(s)	109, 476, 2520, 2691, 3241
Bst1107I	GTATAC	1 cut(s)	3376
Bst6I	CTCTTC	2 cut(s)	253, 1245
BstAFI	CTTAAG	1 cut(s)	1832
BstAUI	TGTACA	2 cut(s)	1635, 2892
BstBAI	YACGTR	2 cut(s)	3114, 3361
BstBI	TTCGAA	1 cut(s)	1807
BstDSI	CCRYGG	2 cut(s)	476, 1851
BstENI	CCTNNNNNAGG	1 cut(s)	3038
BstF5I	GGATG	7 cut(s)	574, 1398, 1612, 1638, 1720, 1926, 3513
BstFNI	CGCG	5 cut(s)	130, 1231, 2628, 2659, 3662
BstH2I	RGCGCY	1 cut(s)	242
BstHHI	GCGC	4 cut(s)	241, 894, 2609, 3295
BstMAI	GTCTC	4 cut(s)	202, 447, 581, 2635
BstMCI	CGRYCG	1 cut(s)	3307
BstMWI	GCNNNNNNNGC	5 cut(s)	157, 247, 1455, 2522, 2786
BstNSI	RCATGY	2 cut(s)	66, 1215
BstSCI	CCNGG	2 cut(s)	118, 2291
BstSFI	CTRYAG	3 cut(s)	1082, 1903, 3568
BstUI	CGCG	5 cut(s)	130, 1231, 2628, 2659, 3662
BstV2I	GAAGAC	2 cut(s)	2247, 2953
BstX2I	RGATCY	4 cut(s)	916, 1039, 1640, 3318
BstYI	RGATCY	4 cut(s)	916, 1039, 1640, 3318
BstZ17I	GTATAC	1 cut(s)	3376
Bsu36I	CCTNAGG	1 cut(s)	1065
BsuRI	GGCC	8 cut(s)	114, 167, 651, 2144, 2789, 2984, 3219, 3438
BtgI	CCRYGG	2 cut(s)	476, 1851
BtgZI	GCGATG	1 cut(s)	2459
BtrI	CACGTC	1 cut(s)	1302
BtsCI	GGATG	7 cut(s)	574, 1398, 1612, 1638, 1720, 1926, 3513
BtsI	GCAGTG	2 cut(s)	390, 3565
BtsIMutI	CAGTG	9 cut(s)	390, 810, 959, 1491, 2764, 2797, 3565, 3726, 3746
BveI	ACCTGC	1 cut(s)	3269
CciI	TCATGA	2 cut(s)	219, 3348
CfoI	GCGC	4 cut(s)	241, 894, 2609, 3295
Cfr10I	RCCGGY	2 cut(s)	2315, 3306
Cfr13I	GGNCC	6 cut(s)	703, 845, 2159, 2578, 3273, 3437
CseI	GACGC	2 cut(s)	2617, 3668
Csp6I	GTAC	7 cut(s)	181, 211, 1636, 1876, 2893, 3118, 3358
CspAI	ACCGGT	2 cut(s)	2315, 3306
CviQI	GTAC	7 cut(s)	181, 211, 1636, 1876, 2893, 3118, 3358
DraI	TTTAAA	2 cut(s)	1245, 2463
DriI	GACNNNNNGTC	1 cut(s)	3729
EaeI	YGGCCR	4 cut(s)	112, 2142, 2787, 3217
Eam1104I	CTCTTC	2 cut(s)	253, 1245
Eam1105I	GACNNNNNGTC	1 cut(s)	3729
EarI	CTCTTC	2 cut(s)	253, 1245
Eco130I	CCWWGG	5 cut(s)	109, 476, 2520, 2691, 3241
Eco24I	GRGCYC	2 cut(s)	1099, 2916
Eco31I	GGTCTC	1 cut(s)	581
Eco47I	GGWCC	5 cut(s)	703, 845, 2159, 2578, 3273
Eco47III	AGCGCT	1 cut(s)	240
Eco57I	CTGAAG	4 cut(s)	52, 2459, 2510, 2517
Eco72I	CACGTG	1 cut(s)	3114
Eco81I	CCTNAGG	1 cut(s)	1065
Eco88I	CYCGRG	2 cut(s)	1552, 3651
EcoNI	CCTNNNNNAGG	1 cut(s)	3038
EcoO109I	RGGNCCY	2 cut(s)	703, 2159
EcoRI	GAATTC	2 cut(s)	1316, 3666
EcoT14I	CCWWGG	5 cut(s)	109, 476, 2520, 2691, 3241
EcoT22I	ATGCAT	2 cut(s)	757, 2273
EcoT38I	GRGCYC	2 cut(s)	1099, 2916
ErhI	CCWWGG	5 cut(s)	109, 476, 2520, 2691, 3241
Esp3I	CGTCTC	2 cut(s)	447, 2635
FalI	AAGNNNNNCTT	2 cut(s)	630, 662
FaqI	GGGAC	2 cut(s)	1306, 2145
FauI	CCCGC	1 cut(s)	123
FauNDI	CATATG	3 cut(s)	575, 2618, 3335
FbaI	TGATCA	3 cut(s)	372, 661, 3297
FblI	GTMKAC	1 cut(s)	3375
FokI	GGATG	7 cut(s)	581, 1405, 1619, 1645, 1727, 1933, 3500
FriOI	GRGCYC	2 cut(s)	1099, 2916
FspBI	CTAG	7 cut(s)	534, 1028, 1508, 1772, 2180, 3161, 3766
GlaI	GCGC	4 cut(s)	240, 893, 2608, 3294
GsaI	CCCAGC	1 cut(s)	3764
GsuI	CTGGAG	1 cut(s)	3050
HaeII	RGCGCY	1 cut(s)	242
HaeIII	GGCC	8 cut(s)	114, 167, 651, 2144, 2789, 2984, 3219, 3438
HapII	CCGG	8 cut(s)	119, 1754, 2092, 2292, 2316, 2368, 3121, 3307
HgaI	GACGC	2 cut(s)	2617, 3668
HhaI	GCGC	4 cut(s)	241, 894, 2609, 3295
Hin6I	GCGC	4 cut(s)	239, 892, 2607, 3293
HinP1I	GCGC	4 cut(s)	239, 892, 2607, 3293
HincII	GTYRAC	2 cut(s)	514, 3658
HindII	GTYRAC	2 cut(s)	514, 3658
HindIII	AAGCTT	2 cut(s)	800, 2279
HpaI	GTTAAC	1 cut(s)	514
HpaII	CCGG	8 cut(s)	119, 1754, 2092, 2292, 2316, 2368, 3121, 3307
HphI	GGTGA	6 cut(s)	873, 1367, 1493, 1743, 2305, 2330
Hpy99I	CGWCG	2 cut(s)	1943, 2972
HpyCH4IV	ACGT	6 cut(s)	447, 1301, 1566, 3045, 3113, 3360
HpyF10VI	GCNNNNNNNGC	5 cut(s)	157, 247, 1455, 2522, 2786
HpySE526I	ACGT	6 cut(s)	447, 1301, 1566, 3045, 3113, 3360
HspAI	GCGC	4 cut(s)	239, 892, 2607, 3293
KpnI	GGTACC	1 cut(s)	3121
Ksp22I	TGATCA	3 cut(s)	372, 661, 3297
KspAI	GTTAAC	1 cut(s)	514
LguI	GCTCTTC	1 cut(s)	1245
LmnI	GCTCC	5 cut(s)	42, 111, 319, 346, 2370
LweI	GCATC	6 cut(s)	620, 764, 933, 1391, 2198, 3354
MaeI	CTAG	7 cut(s)	534, 1028, 1508, 1772, 2180, 3161, 3766
MaeII	ACGT	6 cut(s)	447, 1301, 1566, 3045, 3113, 3360
MfeI	CAATTG	2 cut(s)	2697, 3280
MflI	RGATCY	4 cut(s)	916, 1039, 1640, 3318
MhlI	GDGCHC	2 cut(s)	1099, 2916
MlsI	TGGCCA	4 cut(s)	114, 2144, 2789, 3219
MluNI	TGGCCA	4 cut(s)	114, 2144, 2789, 3219
MlyI	GAGTC	5 cut(s)	1418, 1683, 1757, 3039, 3625
MmeI	TCCRAC	8 cut(s)	430, 878, 1668, 2447, 3113, 3346, 3516, 3541
Mox20I	TGGCCA	4 cut(s)	114, 2144, 2789, 3219
Mph1103I	ATGCAT	2 cut(s)	757, 2273
MroXI	GAANNNNTTC	2 cut(s)	528, 2478
MscI	TGGCCA	4 cut(s)	114, 2144, 2789, 3219
MseI	TTAA	8 cut(s)	513, 624, 1008, 1179, 1244, 1833, 2462, 2601
MslI	CAYNNNNRTG	2 cut(s)	3243, 3626
Msp20I	TGGCCA	4 cut(s)	114, 2144, 2789, 3219
MspA1I	CMGCKG	2 cut(s)	290, 696
MspCI	CTTAAG	1 cut(s)	1832
MspI	CCGG	8 cut(s)	119, 1754, 2092, 2292, 2316, 2368, 3121, 3307
MspR9I	CCNGG	2 cut(s)	120, 2293
MunI	CAATTG	2 cut(s)	2697, 3280
Mva1269I	GAATGC	3 cut(s)	2273, 2778, 3383
MvnI	CGCG	5 cut(s)	130, 1231, 2628, 2659, 3662
MwoI	GCNNNNNNNGC	5 cut(s)	157, 247, 1455, 2522, 2786
NciI	CCSGG	2 cut(s)	120, 2293
NcoI	CCATGG	1 cut(s)	476
NdeI	CATATG	3 cut(s)	575, 2618, 3335
NlaIV	GGNNCC	7 cut(s)	315, 705, 2161, 2262, 3119, 3247, 3320
NsiI	ATGCAT	2 cut(s)	757, 2273
NspI	RCATGY	2 cut(s)	66, 1215
NspV	TTCGAA	1 cut(s)	1807
OliI	CACNNNNGTG	1 cut(s)	3243
PaeI	GCATGC	1 cut(s)	66
PaeR7I	CTCGAG	1 cut(s)	1552
PagI	TCATGA	2 cut(s)	219, 3348
PaqCI	CACCTGC	1 cut(s)	3269
PciI	ACATGT	1 cut(s)	1211
PciSI	GCTCTTC	1 cut(s)	1245
PcsI	WCGNNNNNNNCGW	1 cut(s)	1559
PctI	GAATGC	3 cut(s)	2273, 2778, 3383
PdmI	GAANNNNTTC	2 cut(s)	528, 2478
PinAI	ACCGGT	2 cut(s)	2315, 3306
PleI	GAGTC	5 cut(s)	1417, 1682, 1756, 3038, 3625
PmaCI	CACGTG	1 cut(s)	3114
PmlI	CACGTG	1 cut(s)	3114
PpsI	GAGTC	5 cut(s)	1417, 1682, 1756, 3038, 3625
Ppu21I	YACGTR	2 cut(s)	3114, 3361
PpuMI	RGGWCCY	2 cut(s)	703, 2159
PscI	ACATGT	1 cut(s)	1211
Psp5II	RGGWCCY	2 cut(s)	703, 2159
PspCI	CACGTG	1 cut(s)	3114
PspFI	CCCAGC	1 cut(s)	3760
PspN4I	GGNNCC	7 cut(s)	315, 705, 2161, 2262, 3119, 3247, 3320
PspPI	GGNCC	6 cut(s)	703, 845, 2159, 2578, 3273, 3437
PspPPI	RGGWCCY	2 cut(s)	703, 2159
PstI	CTGCAG	1 cut(s)	3572
PsuI	RGATCY	4 cut(s)	916, 1039, 1640, 3318
PvuII	CAGCTG	1 cut(s)	290
RsaI	GTAC	7 cut(s)	182, 212, 1637, 1877, 2894, 3119, 3359
RsaNI	GTAC	7 cut(s)	181, 211, 1636, 1876, 2893, 3118, 3358
RseI	CAYNNNNRTG	2 cut(s)	3243, 3626
SapI	GCTCTTC	1 cut(s)	1245
SaqAI	TTAA	8 cut(s)	513, 624, 1008, 1179, 1244, 1833, 2462, 2601
Sau96I	GGNCC	6 cut(s)	703, 845, 2159, 2578, 3273, 3437
SchI	GAGTC	5 cut(s)	1418, 1683, 1757, 3039, 3625
ScrFI	CCNGG	2 cut(s)	120, 2293
SduI	GDGCHC	2 cut(s)	1099, 2916
SfaNI	GCATC	6 cut(s)	620, 764, 933, 1391, 2198, 3354
SfcI	CTRYAG	3 cut(s)	1082, 1903, 3568
Sfr274I	CTCGAG	1 cut(s)	1552
SfuI	TTCGAA	1 cut(s)	1807
SgrAI	CRCCGGYG	1 cut(s)	2315
SinI	GGWCC	5 cut(s)	703, 845, 2159, 2578, 3273
SlaI	CTCGAG	1 cut(s)	1552
SmiI	ATTTAAAT	1 cut(s)	1245
SmiMI	CAYNNNNRTG	2 cut(s)	3243, 3626
SmlI	CTYRAG	8 cut(s)	427, 617, 1552, 1782, 1832, 2099, 2941, 3035
SmoI	CTYRAG	8 cut(s)	427, 617, 1552, 1782, 1832, 2099, 2941, 3035
SphI	GCATGC	1 cut(s)	66
SsiI	CCGC	4 cut(s)	130, 694, 2071, 2626
SspI	AATATT	3 cut(s)	527, 2133, 3738
SspMI	CTAG	7 cut(s)	534, 1028, 1508, 1772, 2180, 3161, 3766
StyD4I	CCNGG	2 cut(s)	118, 2291
StyI	CCWWGG	5 cut(s)	109, 476, 2520, 2691, 3241
SwaI	ATTTAAAT	1 cut(s)	1245
TaiI	ACGT	6 cut(s)	450, 1304, 1569, 3048, 3116, 3363
TatI	WGTACW	4 cut(s)	210, 1635, 1875, 2892
TauI	GCSGC	1 cut(s)	2628
Tru1I	TTAA	8 cut(s)	513, 624, 1008, 1179, 1244, 1833, 2462, 2601
Tru9I	TTAA	8 cut(s)	513, 624, 1008, 1179, 1244, 1833, 2462, 2601
TscAI	CASTG	9 cut(s)	390, 817, 966, 1498, 2764, 2797, 3572, 3726, 3753
TspGWI	ACGGA	4 cut(s)	289, 1013, 1405, 3522
TspRI	CASTG	9 cut(s)	390, 817, 966, 1498, 2764, 2797, 3572, 3726, 3753
Vha464I	CTTAAG	1 cut(s)	1832
VpaK11BI	GGWCC	5 cut(s)	703, 845, 2159, 2578, 3273
XagI	CCTNNNNNAGG	1 cut(s)	3038
XapI	RAATTY	6 cut(s)	461, 1240, 1316, 1341, 1838, 3666
XbaI	TCTAGA	1 cut(s)	1027
XceI	RCATGY	2 cut(s)	66, 1215
XcmI	CCANNNNNNNNNTGG	1 cut(s)	107
XhoI	CTCGAG	1 cut(s)	1552
XmiI	GTMKAC	1 cut(s)	3375
XmnI	GAANNNNTTC	2 cut(s)	528, 2478
XspI	CTAG	7 cut(s)	534, 1028, 1508, 1772, 2180, 3161, 3766
Zsp2I	ATGCAT	2 cut(s)	757, 2273

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.