Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily
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| Species | Orthologous Gene IDs |
|---|---|
| fragaria_vesca | FvH4_3g11830 |
| malus_domestica | MD10G1238400.v1.1 |
| prunus_persica | Prupe.4G105900_v2.0.a1 |
| pyrus_communis | pycom10g19940 |
| rosa_chinensis | RchiOBHm_Chr5g0019261 |
| rosa_laevigata | RLG00000032432 |
| rosa_multiflora | Rmu_co8517133.1_g000001 |
| rosa_roxburghii | Rroxscaffold_1G00058740 |
| rosa_rugosa | Rorug05G0049000 |
| rosa_samantha | Rh5AG140100 Rh5BG139100 Rh5CG150500 Rh5DG139700 |
| rosa_wichuraiana | Rw5G012460 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 1588 |
| Acc65I | GGTACC | 1 cut(s) | 1020 |
| AccB1I | GGYRCC | 3 cut(s) | 399, 1020, 2816 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 1017, 3024 |
| AccI | GTMKAC | 1 cut(s) | 2481 |
| AciI | CCGC | 3 cut(s) | 1484, 1568, 2665 |
| AclWI | GGATC | 9 cut(s) | 89, 701, 714, 927, 1117, 1835, 2884, 2897, 3079 |
| AcoI | YGGCCR | 3 cut(s) | 100, 1008, 2139 |
| AcsI | RAATTY | 6 cut(s) | 427, 680, 739, 1319, 1436, 1895 |
| AcuI | CTGAAG | 2 cut(s) | 52, 2061 |
| AfaI | GTAC | 6 cut(s) | 185, 215, 1022, 1874, 2438, 2930 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 735, 1017, 1709, 1850, 2365, 3024 |
| AflIII | ACRYGT | 1 cut(s) | 1304 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 3330 |
| AjiI | CACGTC | 2 cut(s) | 1305, 2111 |
| AjnI | CCWGG | 3 cut(s) | 97, 1066, 1983 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 230, 262, 263, 295, 2271, 2303 |
| AleI | CACNNNNGTG | 1 cut(s) | 2814 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 185, 217 |
| Alw21I | GWGCWC | 1 cut(s) | 1909 |
| Alw26I | GTCTC | 5 cut(s) | 306, 584, 1147, 2101, 2181 |
| AlwI | GGATC | 9 cut(s) | 89, 701, 714, 927, 1117, 1835, 2884, 2897, 3079 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 112 |
| Ama87I | CYCGRG | 2 cut(s) | 132, 1537 |
| AoxI | GGCC | 8 cut(s) | 100, 687, 1008, 1098, 2139, 2455, 2635, 3007 |
| ApaI | GGGCCC | 1 cut(s) | 1102 |
| ApeKI | GCWGC | 7 cut(s) | 160, 290, 1382, 1505, 1775, 2166, 2913 |
| ApoI | RAATTY | 6 cut(s) | 427, 680, 739, 1319, 1436, 1895 |
| AseI | ATTAAT | 1 cut(s) | 1365 |
| Asp700I | GAANNNNTTC | 4 cut(s) | 531, 1953, 2022, 2774 |
| Asp718I | GGTACC | 1 cut(s) | 1020 |
| AspLEI | GCGC | 1 cut(s) | 2956 |
| AspS9I | GGNCC | 7 cut(s) | 848, 1098, 1099, 1641, 2122, 2635, 3008 |
| AsuC2I | CCSGG | 3 cut(s) | 123, 133, 134 |
| AsuHPI | GGTGA | 6 cut(s) | 876, 1000, 1724, 1740, 1958, 2866 |
| AsuII | TTCGAA | 3 cut(s) | 800, 2414, 2972 |
| AvaI | CYCGRG | 2 cut(s) | 132, 1537 |
| AvaII | GGWCC | 3 cut(s) | 848, 1641, 2122 |
| BaeGI | GKGCMC | 3 cut(s) | 776, 1102, 2394 |
| BalI | TGGCCA | 2 cut(s) | 102, 1010 |
| BamHI | GGATCC | 2 cut(s) | 706, 2889 |
| BanI | GGYRCC | 3 cut(s) | 399, 1020, 2816 |
| BanII | GRGCYC | 2 cut(s) | 448, 1102 |
| BbsI | GAAGAC | 1 cut(s) | 2004 |
| Bbv12I | GWGCWC | 1 cut(s) | 1909 |
| BbvCI | CCTCAGC | 1 cut(s) | 156 |
| BbvI | GCAGC | 7 cut(s) | 147, 302, 1394, 1492, 1762, 2153, 2925 |
| BceAI | ACGGC | 2 cut(s) | 2126, 2704 |
| BciT130I | CCWGG | 3 cut(s) | 99, 1068, 1985 |
| BciVI | GTATCC | 2 cut(s) | 211, 2673 |
| BclI | TGATCA | 3 cut(s) | 664, 1042, 1714 |
| BcnI | CCSGG | 3 cut(s) | 123, 133, 134 |
| BcoDI | GTCTC | 5 cut(s) | 306, 584, 1147, 2101, 2181 |
| BfaI | CTAG | 8 cut(s) | 287, 561, 1563, 1892, 2334, 2705, 3041, 3225 |
| BfmI | CTRYAG | 3 cut(s) | 1146, 1900, 2167 |
| BfuAI | ACCTGC | 1 cut(s) | 1588 |
| BfuI | GTATCC | 2 cut(s) | 211, 2673 |
| BisI | GCNGC | 8 cut(s) | 161, 291, 1383, 1506, 1776, 2167, 2665, 2914 |
| BlpI | GCTNAGC | 1 cut(s) | 171 |
| BlsI | GCNGC | 8 cut(s) | 162, 292, 1384, 1507, 1777, 2168, 2666, 2915 |
| Bme1390I | CCNGG | 6 cut(s) | 99, 123, 133, 134, 1068, 1985 |
| Bme18I | GGWCC | 3 cut(s) | 848, 1641, 2122 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 3330 |
| BmeT110I | CYCGRG | 2 cut(s) | 132, 1537 |
| BmgBI | CACGTC | 2 cut(s) | 1305, 2111 |
| BmgT120I | GGNCC | 7 cut(s) | 848, 1098, 1099, 1641, 2122, 2635, 3008 |
| BmiI | GGNNCC | 7 cut(s) | 401, 708, 1022, 1100, 2663, 2818, 2891 |
| BmrFI | CCNGG | 6 cut(s) | 99, 123, 133, 134, 1068, 1985 |
| BmrI | ACTGGG | 1 cut(s) | 122 |
| BmsI | GCATC | 7 cut(s) | 53, 1394, 2047, 2676, 2708, 2925, 3223 |
| BmuI | ACTGGG | 1 cut(s) | 122 |
| BpiI | GAAGAC | 1 cut(s) | 2004 |
| BpmI | CTGGAG | 2 cut(s) | 1089, 1362 |
| Bpu10I | CCTNAGC | 1 cut(s) | 156 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 171 |
| Bpu14I | TTCGAA | 3 cut(s) | 800, 2414, 2972 |
| BpuEI | CTTGAG | 4 cut(s) | 290, 1814, 2594, 2600 |
| BpuMI | CCSGG | 3 cut(s) | 123, 133, 134 |
| Bsa29I | ATCGAT | 1 cut(s) | 1374 |
| BsaBI | GATNNNNATC | 5 cut(s) | 93, 711, 886, 1041, 1713 |
| BsaI | GGTCTC | 1 cut(s) | 584 |
| BsaJI | CCNNGG | 5 cut(s) | 122, 132, 2064, 2235, 2812 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 492, 522 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 735, 1017, 1709, 1850, 2365, 3024 |
| Bse1I | ACTGG | 6 cut(s) | 117, 982, 2125, 2399, 2522, 3029 |
| Bse3DI | GCAATG | 6 cut(s) | 154, 1942, 2345, 2445, 3035, 3078 |
| Bse8I | GATNNNNATC | 5 cut(s) | 93, 711, 886, 1041, 1713 |
| BseBI | CCWGG | 3 cut(s) | 99, 1068, 1985 |
| BseCI | ATCGAT | 1 cut(s) | 1374 |
| BseDI | CCNNGG | 5 cut(s) | 122, 132, 2064, 2235, 2812 |
| BseJI | GATNNNNATC | 5 cut(s) | 93, 711, 886, 1041, 1713 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 735, 1017, 1709, 1850, 2365, 3024 |
| BseMI | GCAATG | 6 cut(s) | 154, 1942, 2345, 2445, 3035, 3078 |
| BseMII | CTCAG | 5 cut(s) | 170, 651, 2096, 2508, 3207 |
| BseNI | ACTGG | 6 cut(s) | 117, 982, 2125, 2399, 2522, 3029 |
| BseSI | GKGCMC | 3 cut(s) | 776, 1102, 2394 |
| BseXI | GCAGC | 7 cut(s) | 147, 302, 1394, 1492, 1762, 2153, 2925 |
| BsgI | GTGCAG | 5 cut(s) | 146, 1401, 1563, 1761, 2997 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1378 |
| BshFI | GGCC | 8 cut(s) | 102, 689, 1010, 1100, 2141, 2457, 2637, 3009 |
| BshNI | GGYRCC | 3 cut(s) | 399, 1020, 2816 |
| BshVI | ATCGAT | 1 cut(s) | 1374 |
| BsiEI | CGRYCG | 1 cut(s) | 1378 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1909 |
| BsiHKCI | CYCGRG | 2 cut(s) | 132, 1537 |
| BsiSI | CCGG | 3 cut(s) | 122, 133, 2618 |
| BslFI | GGGAC | 2 cut(s) | 1309, 3288 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 735, 1017, 1709, 1850, 2365, 3024 |
| BsmAI | GTCTC | 5 cut(s) | 306, 584, 1147, 2101, 2181 |
| BsmBI | CGTCTC | 1 cut(s) | 2101 |
| BsmFI | GGGAC | 2 cut(s) | 1309, 3288 |
| BsmI | GAATGC | 1 cut(s) | 2322 |
| BsnI | GGCC | 8 cut(s) | 102, 689, 1010, 1100, 2141, 2457, 2637, 3009 |
| Bso31I | GGTCTC | 1 cut(s) | 584 |
| BsoBI | CYCGRG | 2 cut(s) | 132, 1537 |
| Bsp119I | TTCGAA | 3 cut(s) | 800, 2414, 2972 |
| Bsp120I | GGGCCC | 1 cut(s) | 1098 |
| Bsp1286I | GDGCHC | 5 cut(s) | 448, 776, 1102, 1909, 2394 |
| Bsp1407I | TGTACA | 1 cut(s) | 2436 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 171 |
| BspACI | CCGC | 3 cut(s) | 1484, 1568, 2665 |
| BspANI | GGCC | 8 cut(s) | 102, 689, 1010, 1100, 2141, 2457, 2637, 3009 |
| BspCNI | CTCAG | 5 cut(s) | 169, 650, 2097, 2507, 3206 |
| BspDI | ATCGAT | 1 cut(s) | 1374 |
| BspLI | GGNNCC | 7 cut(s) | 401, 708, 1022, 1100, 2663, 2818, 2891 |
| BspMAI | CTGCAG | 1 cut(s) | 2171 |
| BspMI | ACCTGC | 1 cut(s) | 1588 |
| BspPI | GGATC | 9 cut(s) | 89, 701, 714, 927, 1117, 1835, 2884, 2897, 3079 |
| BspQI | GCTCTTC | 1 cut(s) | 3063 |
| BspT104I | TTCGAA | 3 cut(s) | 800, 2414, 2972 |
| BspT107I | GGYRCC | 3 cut(s) | 399, 1020, 2816 |
| BspTNI | GGTCTC | 1 cut(s) | 584 |
| BsrDI | GCAATG | 6 cut(s) | 154, 1942, 2345, 2445, 3035, 3078 |
| BsrGI | TGTACA | 1 cut(s) | 2436 |
| BsrI | ACTGG | 6 cut(s) | 117, 982, 2125, 2399, 2522, 3029 |
| BssECI | CCNNGG | 5 cut(s) | 122, 132, 2064, 2235, 2812 |
| BssNAI | GTATAC | 1 cut(s) | 2482 |
| BssT1I | CCWWGG | 2 cut(s) | 2064, 2235 |
| Bst1107I | GTATAC | 1 cut(s) | 2482 |
| Bst2UI | CCWGG | 3 cut(s) | 99, 1068, 1985 |
| Bst6I | CTCTTC | 2 cut(s) | 2906, 3063 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1174 |
| BstAUI | TGTACA | 1 cut(s) | 2436 |
| BstBI | TTCGAA | 3 cut(s) | 800, 2414, 2972 |
| BstC8I | GCNNGC | 3 cut(s) | 47, 1170, 2322 |
| BstDEI | CTNAG | 8 cut(s) | 156, 171, 637, 791, 806, 2105, 2494, 3193 |
| BstHHI | GCGC | 1 cut(s) | 2956 |
| BstMAI | GTCTC | 5 cut(s) | 306, 584, 1147, 2101, 2181 |
| BstMCI | CGRYCG | 1 cut(s) | 1378 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 1174, 1511, 1726, 2066, 2330, 2771 |
| BstNI | CCWGG | 3 cut(s) | 99, 1068, 1985 |
| BstNSI | RCATGY | 3 cut(s) | 1218, 1531, 2835 |
| BstSCI | CCNGG | 6 cut(s) | 97, 121, 131, 132, 1066, 1983 |
| BstSFI | CTRYAG | 3 cut(s) | 1146, 1900, 2167 |
| BstSLI | GKGCMC | 3 cut(s) | 776, 1102, 2394 |
| BstV1I | GCAGC | 7 cut(s) | 147, 302, 1394, 1492, 1762, 2153, 2925 |
| BstV2I | GAAGAC | 1 cut(s) | 2004 |
| BstX2I | RGATCY | 3 cut(s) | 706, 919, 2889 |
| BstYI | RGATCY | 3 cut(s) | 706, 919, 2889 |
| BstZ17I | GTATAC | 1 cut(s) | 2482 |
| Bsu15I | ATCGAT | 1 cut(s) | 1374 |
| BsuI | GTATCC | 2 cut(s) | 211, 2673 |
| BsuRI | GGCC | 8 cut(s) | 102, 689, 1010, 1100, 2141, 2457, 2637, 3009 |
| BsuTUI | ATCGAT | 1 cut(s) | 1374 |
| BtrI | CACGTC | 2 cut(s) | 1305, 2111 |
| BtsI | GCAGTG | 2 cut(s) | 782, 870 |
| BtsIMutI | CAGTG | 9 cut(s) | 782, 870, 1485, 1494, 2308, 2392, 2462, 3201, 3320 |
| BveI | ACCTGC | 1 cut(s) | 1588 |
| Cac8I | GCNNGC | 3 cut(s) | 47, 1170, 2322 |
| CaiI | CAGNNNCTG | 1 cut(s) | 112 |
| CfoI | GCGC | 1 cut(s) | 2956 |
| Cfr13I | GGNCC | 7 cut(s) | 848, 1098, 1099, 1641, 2122, 2635, 3008 |
| Cfr9I | CCCGGG | 1 cut(s) | 132 |
| ClaI | ATCGAT | 1 cut(s) | 1374 |
| Csp6I | GTAC | 6 cut(s) | 184, 214, 1021, 1873, 2437, 2929 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 2867, 2902 |
| CviQI | GTAC | 6 cut(s) | 184, 214, 1021, 1873, 2437, 2929 |
| DdeI | CTNAG | 8 cut(s) | 156, 171, 637, 791, 806, 2105, 2494, 3193 |
| DraI | TTTAAA | 3 cut(s) | 1054, 1884, 2007 |
| DriI | GACNNNNNGTC | 1 cut(s) | 3330 |
| EaeI | YGGCCR | 3 cut(s) | 100, 1008, 2139 |
| Eam1104I | CTCTTC | 2 cut(s) | 2906, 3063 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 3330 |
| EarI | CTCTTC | 2 cut(s) | 2906, 3063 |
| EciI | GGCGGA | 1 cut(s) | 1499 |
| Eco130I | CCWWGG | 2 cut(s) | 2064, 2235 |
| Eco24I | GRGCYC | 2 cut(s) | 448, 1102 |
| Eco31I | GGTCTC | 1 cut(s) | 584 |
| Eco32I | GATATC | 1 cut(s) | 2862 |
| Eco47I | GGWCC | 3 cut(s) | 848, 1641, 2122 |
| Eco57I | CTGAAG | 2 cut(s) | 52, 2061 |
| Eco88I | CYCGRG | 2 cut(s) | 132, 1537 |
| EcoO109I | RGGNCCY | 2 cut(s) | 1098, 1099 |
| EcoRI | GAATTC | 3 cut(s) | 427, 680, 1895 |
| EcoRII | CCWGG | 3 cut(s) | 97, 1066, 1983 |
| EcoRV | GATATC | 1 cut(s) | 2862 |
| EcoT14I | CCWWGG | 2 cut(s) | 2064, 2235 |
| EcoT22I | ATGCAT | 1 cut(s) | 2837 |
| EcoT38I | GRGCYC | 2 cut(s) | 448, 1102 |
| ErhI | CCWWGG | 2 cut(s) | 2064, 2235 |
| Esp3I | CGTCTC | 1 cut(s) | 2101 |
| FalI | AAGNNNNNCTT | 8 cut(s) | 633, 665, 1494, 1526, 2219, 2251, 3245, 3277 |
| FaqI | GGGAC | 2 cut(s) | 1309, 3288 |
| FauNDI | CATATG | 4 cut(s) | 578, 1351, 2726, 2906 |
| FbaI | TGATCA | 3 cut(s) | 664, 1042, 1714 |
| FblI | GTMKAC | 1 cut(s) | 2481 |
| Fnu4HI | GCNGC | 8 cut(s) | 161, 291, 1383, 1506, 1776, 2167, 2665, 2914 |
| FriOI | GRGCYC | 2 cut(s) | 448, 1102 |
| Fsp4HI | GCNGC | 8 cut(s) | 161, 291, 1383, 1506, 1776, 2167, 2665, 2914 |
| FspBI | CTAG | 8 cut(s) | 287, 561, 1563, 1892, 2334, 2705, 3041, 3225 |
| GlaI | GCGC | 1 cut(s) | 2955 |
| GluI | GCNGC | 8 cut(s) | 161, 291, 1383, 1506, 1776, 2167, 2665, 2914 |
| GsuI | CTGGAG | 2 cut(s) | 1089, 1362 |
| HaeIII | GGCC | 8 cut(s) | 102, 689, 1010, 1100, 2141, 2457, 2637, 3009 |
| HapII | CCGG | 3 cut(s) | 122, 133, 2618 |
| HhaI | GCGC | 1 cut(s) | 2956 |
| Hin6I | GCGC | 1 cut(s) | 2954 |
| HinP1I | GCGC | 1 cut(s) | 2954 |
| HincII | GTYRAC | 2 cut(s) | 119, 517 |
| HindII | GTYRAC | 2 cut(s) | 119, 517 |
| HindIII | AAGCTT | 1 cut(s) | 1456 |
| HpaI | GTTAAC | 1 cut(s) | 517 |
| HpaII | CCGG | 3 cut(s) | 122, 133, 2618 |
| HphI | GGTGA | 6 cut(s) | 876, 1000, 1724, 1740, 1958, 2866 |
| Hpy166II | GTNNAC | 5 cut(s) | 119, 517, 848, 2437, 2482 |
| Hpy8I | GTNNAC | 5 cut(s) | 119, 517, 848, 2437, 2482 |
| HpyCH4IV | ACGT | 2 cut(s) | 1304, 2110 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 1174, 1511, 1726, 2066, 2330, 2771 |
| HpyF3I | CTNAG | 8 cut(s) | 156, 171, 637, 791, 806, 2105, 2494, 3193 |
| HpySE526I | ACGT | 2 cut(s) | 1304, 2110 |
| HspAI | GCGC | 1 cut(s) | 2954 |
| KpnI | GGTACC | 1 cut(s) | 1024 |
| Ksp22I | TGATCA | 3 cut(s) | 664, 1042, 1714 |
| KspAI | GTTAAC | 1 cut(s) | 517 |
| LguI | GCTCTTC | 1 cut(s) | 3063 |
| LmnI | GCTCC | 5 cut(s) | 42, 322, 349, 1343, 2661 |
| Lsp1109I | GCAGC | 7 cut(s) | 147, 302, 1394, 1492, 1762, 2153, 2925 |
| LweI | GCATC | 7 cut(s) | 53, 1394, 2047, 2676, 2708, 2925, 3223 |
| MaeI | CTAG | 8 cut(s) | 287, 561, 1563, 1892, 2334, 2705, 3041, 3225 |
| MaeII | ACGT | 2 cut(s) | 1304, 2110 |
| MfeI | CAATTG | 1 cut(s) | 2851 |
| MflI | RGATCY | 3 cut(s) | 706, 919, 2889 |
| MhlI | GDGCHC | 5 cut(s) | 448, 776, 1102, 1909, 2394 |
| MlsI | TGGCCA | 2 cut(s) | 102, 1010 |
| MluNI | TGGCCA | 2 cut(s) | 102, 1010 |
| MlyI | GAGTC | 4 cut(s) | 1421, 1683, 1754, 2583 |
| MmeI | TCCRAC | 8 cut(s) | 433, 478, 881, 1266, 1668, 2657, 2917, 3112 |
| Mox20I | TGGCCA | 2 cut(s) | 102, 1010 |
| Mph1103I | ATGCAT | 1 cut(s) | 2837 |
| MroXI | GAANNNNTTC | 4 cut(s) | 531, 1953, 2022, 2774 |
| MscI | TGGCCA | 2 cut(s) | 102, 1010 |
| MslI | CAYNNNNRTG | 2 cut(s) | 2814, 2830 |
| Msp20I | TGGCCA | 2 cut(s) | 102, 1010 |
| MspA1I | CMGCKG | 1 cut(s) | 160 |
| MspI | CCGG | 3 cut(s) | 122, 133, 2618 |
| MspR9I | CCNGG | 6 cut(s) | 99, 123, 133, 134, 1068, 1985 |
| MunI | CAATTG | 1 cut(s) | 2851 |
| Mva1269I | GAATGC | 1 cut(s) | 2322 |
| MvaI | CCWGG | 3 cut(s) | 99, 1068, 1985 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 1174, 1511, 1726, 2066, 2330, 2771 |
| NciI | CCSGG | 3 cut(s) | 123, 133, 134 |
| NdeI | CATATG | 4 cut(s) | 578, 1351, 2726, 2906 |
| NlaIV | GGNNCC | 7 cut(s) | 401, 708, 1022, 1100, 2663, 2818, 2891 |
| NmuCI | GTSAC | 6 cut(s) | 223, 1688, 1746, 2336, 3031, 3318 |
| NsiI | ATGCAT | 1 cut(s) | 2837 |
| NspI | RCATGY | 3 cut(s) | 1218, 1531, 2835 |
| NspV | TTCGAA | 3 cut(s) | 800, 2414, 2972 |
| OliI | CACNNNNGTG | 1 cut(s) | 2814 |
| PaeR7I | CTCGAG | 1 cut(s) | 1537 |
| PciSI | GCTCTTC | 1 cut(s) | 3063 |
| PctI | GAATGC | 1 cut(s) | 2322 |
| PdmI | GAANNNNTTC | 4 cut(s) | 531, 1953, 2022, 2774 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 1017, 3024 |
| PfoI | TCCNGGA | 1 cut(s) | 1983 |
| PkrI | GCNGC | 8 cut(s) | 162, 292, 1384, 1507, 1777, 2168, 2666, 2915 |
| Ple19I | CGATCG | 1 cut(s) | 1378 |
| PleI | GAGTC | 4 cut(s) | 1420, 1682, 1753, 2582 |
| PpsI | GAGTC | 4 cut(s) | 1420, 1682, 1753, 2582 |
| PshBI | ATTAAT | 1 cut(s) | 1365 |
| Psp6I | CCWGG | 3 cut(s) | 97, 1066, 1983 |
| PspGI | CCWGG | 3 cut(s) | 97, 1066, 1983 |
| PspN4I | GGNNCC | 7 cut(s) | 401, 708, 1022, 1100, 2663, 2818, 2891 |
| PspOMI | GGGCCC | 1 cut(s) | 1098 |
| PspPI | GGNCC | 7 cut(s) | 848, 1098, 1099, 1641, 2122, 2635, 3008 |
| PspXI | VCTCGAGB | 1 cut(s) | 1537 |
| PstI | CTGCAG | 1 cut(s) | 2171 |
| PstNI | CAGNNNCTG | 1 cut(s) | 112 |
| PsuI | RGATCY | 3 cut(s) | 706, 919, 2889 |
| PvuI | CGATCG | 1 cut(s) | 1378 |
| PvuII | CAGCTG | 1 cut(s) | 160 |
| RsaI | GTAC | 6 cut(s) | 185, 215, 1022, 1874, 2438, 2930 |
| RsaNI | GTAC | 6 cut(s) | 184, 214, 1021, 1873, 2437, 2929 |
| RseI | CAYNNNNRTG | 2 cut(s) | 2814, 2830 |
| SapI | GCTCTTC | 1 cut(s) | 3063 |
| SatI | GCNGC | 8 cut(s) | 161, 291, 1383, 1506, 1776, 2167, 2665, 2914 |
| Sau96I | GGNCC | 7 cut(s) | 848, 1098, 1099, 1641, 2122, 2635, 3008 |
| SchI | GAGTC | 4 cut(s) | 1421, 1683, 1754, 2583 |
| ScrFI | CCNGG | 6 cut(s) | 99, 123, 133, 134, 1068, 1985 |
| SduI | GDGCHC | 5 cut(s) | 448, 776, 1102, 1909, 2394 |
| SfaNI | GCATC | 7 cut(s) | 53, 1394, 2047, 2676, 2708, 2925, 3223 |
| SfcI | CTRYAG | 3 cut(s) | 1146, 1900, 2167 |
| Sfr274I | CTCGAG | 1 cut(s) | 1537 |
| SfuI | TTCGAA | 3 cut(s) | 800, 2414, 2972 |
| SinI | GGWCC | 3 cut(s) | 848, 1641, 2122 |
| SlaI | CTCGAG | 1 cut(s) | 1537 |
| SmaI | CCCGGG | 1 cut(s) | 134 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 2814, 2830 |
| SmlI | CTYRAG | 5 cut(s) | 305, 1537, 1829, 2579, 2609 |
| SmoI | CTYRAG | 5 cut(s) | 305, 1537, 1829, 2579, 2609 |
| SsiI | CCGC | 3 cut(s) | 1484, 1568, 2665 |
| SspI | AATATT | 2 cut(s) | 530, 3312 |
| SspMI | CTAG | 8 cut(s) | 287, 561, 1563, 1892, 2334, 2705, 3041, 3225 |
| StyD4I | CCNGG | 6 cut(s) | 97, 121, 131, 132, 1066, 1983 |
| StyI | CCWWGG | 2 cut(s) | 2064, 2235 |
| TaiI | ACGT | 2 cut(s) | 1307, 2113 |
| TatI | WGTACW | 3 cut(s) | 213, 1872, 2436 |
| TauI | GCSGC | 1 cut(s) | 2667 |
| TscAI | CASTG | 9 cut(s) | 789, 870, 1485, 1501, 2308, 2399, 2469, 3201, 3327 |
| TseFI | GTSAC | 6 cut(s) | 223, 1688, 1746, 2336, 3031, 3318 |
| TseI | GCWGC | 7 cut(s) | 160, 290, 1382, 1505, 1775, 2166, 2913 |
| Tsp45I | GTSAC | 6 cut(s) | 223, 1688, 1746, 2336, 3031, 3318 |
| TspGWI | ACGGA | 3 cut(s) | 1298, 1807, 1833 |
| TspMI | CCCGGG | 1 cut(s) | 132 |
| TspRI | CASTG | 9 cut(s) | 789, 870, 1485, 1501, 2308, 2399, 2469, 3201, 3327 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 1017, 3024 |
| VpaK11BI | GGWCC | 3 cut(s) | 848, 1641, 2122 |
| VspI | ATTAAT | 1 cut(s) | 1365 |
| XapI | RAATTY | 6 cut(s) | 427, 680, 739, 1319, 1436, 1895 |
| XbaI | TCTAGA | 1 cut(s) | 1891 |
| XceI | RCATGY | 3 cut(s) | 1218, 1531, 2835 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 652 |
| XhoI | CTCGAG | 1 cut(s) | 1537 |
| XmaI | CCCGGG | 1 cut(s) | 132 |
| XmiI | GTMKAC | 1 cut(s) | 2481 |
| XmnI | GAANNNNTTC | 4 cut(s) | 531, 1953, 2022, 2774 |
| XspI | CTAG | 8 cut(s) | 287, 561, 1563, 1892, 2334, 2705, 3041, 3225 |
| Zsp2I | ATGCAT | 1 cut(s) | 2837 |