Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0016785

Physical Location & Seq

Genomic Coordinates Reverse (-)

14309869 .. 14315097

Gene Structure 5229 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG139100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3786 bp

ATGTCAGGGTCATCTGGAAGAAGAAAGAGGAAGATAAGATGGAGCAAGCTGTATTCCTATGGATGCTTCAGACCACCCCACACTCTTGATTCTGATCCTGGCCAACAGCTACTGGGTCAACCGGGGTTTTCACGGGTTGTTTTCTGCAATGAACCTCAGCTGCACAAGGCTAAGCCTTACAAGTACCCCAATAACTATGTATCCACAACCAAGTACAACATTGTCACTTTTCTTCCCAAAGCTCTTTTCGAGCAGTTTCGCAGAGTTGCCAATCTTTATTTTCTTCTAGCAGCAGTTTTGTCTCTCAATTCTTTCGCTCCCTTTAATCCAATAAGTTTGATTGCTCCTTTGGTGTTTGTTGTGGGAGTTAGCATGATTAAAGAGGCTGTCGAAGATTGGCACCAATTCTTGCAGGATTTGAATGTGAATTCAAGAACTGTAAGGGCTCATGTTGGAGGTGGCTTATTTGTTGAAAAGACATGGCAAGGGCTTTGTGTTGGAGATGTTGTTAAAGTTAACAAGAATGAATATTTTCCAAGTGATCTTCTCTTGCTATCTTCTAGCTTTGAGGATGGTATATGTTATGTCGAGACCATGAACCTCGATGGAGAAACTAATCTGAAAGTTAAGAGAAGCTCAGAAGCCACACTTGACTTGGCAAATGATCAAGCATTTAGTGAATTCAAGGCCACAATTCGCTGTGAGGATCCAAATCCTCATCTTTACACCTTTGTTGGGAATTTAGAGTTGAACAGTGTAACACATCCCTTGTGCCCTGCCACACTGCTTCTAAGAGATTCGAAACTTAGAAATACTGATTACATTTATGGGGTTGTGATCTTTAGTGGACCTGACACAAAAGCAGTGAGGAACTCAACAAGATCACCATCCAAGCGTAGTCGGATTGAAAGAAAGATGGACCTTGTCATTTACCTTCTCTTTTCAATGCTTCTTCTTATTTCACTCATCACTTCGACTGGTTTCGCGGTGTTACTGAAATCACAAATGGTCAAATGGTGGTACCTTTTTCTGCAAGATGATGATCCACTCTTCAAACCATCAGCTCCTGAAGTATCTGGTTTCCAACAATTTGTAAGGGCCCTTATTTTGTATGGCTACTTGATCCCCATTTCTCTCTATGTCTCTATAGAGGTTGTCAAGGTTTTGCAAGCCATGCTCATTAACAAGGATATAGAGTTGTATGATGAAGTTACATGCAAGTCAGTTCAAACCAGAACATCCAACTTGAATGAAGAACTTGGTCAAGTAGAGATGATTCTGACGGATAAAACGGGGACTTTGACGTGTAATCAGATGGAATTTAGGAAATGCTCTATTGCTGGAGCTTCATATGGGGGTAACATTAATAATATCGATCGTGCAGCATCTAAAAGGATGAATGTTGAGGTTGAGTCGTATCGTTTTAGTATGGATGAATTTGAAACACAATGCCAAAGCTTTGAAATGTTTGACTTTTCAGTGGCGGATGTTAGCACTGAAAAAGCTGCTCTTGCTGGACAGAAGCACATGCAGAACTCGAGTGCAGACTGTTCCAGAATCTCTAGTGCGGAGGGAGAACCTGCCATCAAAGGTTTCAACTTTAGGGATGATAGGCTTATGAACAGAAAGTGGGTTTATAGGTCCAACTTATTTGATGTAACAATGTTCTTTAGAGTCATGGCTCTATGTCACACAGGCATACCTGTTGATGGTGATCAGGCTCATAAGCTAAAGTATGAGGCAGAGTCACCAGAAGAAGTATCTTTTCTAATTGCTGCACAAGAGTTTGGATTTCAATTTTTTCAAAGAAGTCAGTCCGTCATGTATCTCAAGGAGTTTGATCCGTCCTCTGGAAAGGTGGTTGAGAGGAAGTACAAACTTTTAAACCTTCTAGAATTCTGTAGTGCTCGAAAGAGGATGTCAGTTATTGTAAGCAATGAAGATGGTGAAATCTTTCTTCTCTGCAAAGGGGCAGATAACATTATCTTTGATAGACTTGCAGACAATGGTAGGTCTTACCAACAGGCTACAACTCTACACCTTTCAGATTATGCAGAAGATGGATTTCGAACTCTAGCATTTGCTTATCGAAAACTTGATGCTGTCGAGTATGAAAAATGGAACTCAATATTTACTGAGGCTAAGACCACAATAGGTCCTGAAAGAGAAGAAGTACTAATAGAAGCATCTGAAATGATTGAAAAGGATTTAATTCTATTAGGGGTTGCTGCTGTTGAAGACAAGTTACAAAAAGGGGTTCCAGAATGCATAGATAAACTTGCACAATCAGGAATGAAAATATGGCTGCTTACTGGTGACAAGAAAGAAACTGCAATAAATATTGGATTTTCTTGCAGCTTACTTCGGGAGGATATGAAACAGTTTCATCTAATTTTGGGCAAAGAAAGTGCAGCTAGTAACCGACTGAAGGAGATGAAGGAAGACATTTTAAACCAACTTGAAGGCTTTCAGAAAGTGAAGTCTGAAGAAAGCAACAAAGATGCACCCTTGGCTTTGATAGTAGATGGAAAAGCTCTTGAAATTGCTCTGAGAAGTGATGTGAAGGACCAGTTCTTACCGTTGGCCGTTGATTGTTCTTCTGTTATATGCTGCAGGGTGTCTCCAAAACAAAAGGCTCTGATTACTCGATTGGTAAAGGAACATACGGGCAAGACAACCTTGGCCATTGGAGATGGGGCAAATGATGTTGGCATGATTCAAGAAGCTGATATTGGAGTAGGAATCAGTGGCATGGAAGGAATGCAGGCAGTCATGGCCAGTGACATCTCATTGCCTCAATTCCGATTTTTGGGGAGATTACTTATTGTTCATGGGCACTGGTGTTACAAGAGGATTTCGAAAATGATTCTCTACTTCGTGTACAAAAACATTGCATTTGGCCTCACACTGTTCTACTATGAAGTATACACAAGTTTCTCAGGGGAGGTCTTATATGATGACTGGTATATGGCACTATTCAATGTAATTTTGACATCTTTGCCTGTTATATCATTGGGAGTCCTTGAGCAAGATGTTTCATCCGAAGTGTGCCTCAAGTTTCCGGCTCTCTACCAACAGGGCCAAAAGAACATATACTTCACTTGGAGCCGGATCATCGGTTGGATACTCAACGGCATTGTAGCATCTCTAGTCATCTTCCTTGCAAACATATGCACACTTTCTACCTTCAACAAAGATGGTAGTTTTGCAGAGAAAGCCTTCGCCAAAGATGGTAATGTTGCAGACATCACACACCTCGGTGCCATGACATACACATGCATAATCTGGACAGTTAATTGCCAGATATCCCTCATCATCACCCACTTCACTTGGATCCAACATCTCTTCATATGGGGCAGCATCATCATCTGGTACATCTTCCTGTTCATCTACGGTGCGCTACCTCCTGACATTTCGAAAAGAGGCTTTCGTGTTCTTGCAGAAGCTCTTGGCCCTGCACCCATTTACTGGCTTGTCACATTGCTAGTTGTGGTTGTTGCTCTTCTTCCTTATTTCATCCACATTGCGATCCAACGGTCGTTTTATCCTTTGGATGACCATGTGATTCAAGAAATGAAGTATTGCAGGAAAGATATTGGGAAAAATAATCTAATTTGGGAGAGGGAACAGAACAACTCAGTGATGATGACCCAGATAGGATTTTCGGCTAGAGTTGATGCAAGGATTCGGATTATGAAGGAAAACTTGAACCAGAAGGGACAGTTGATATATAGATCAGTAACAAGCAGTCCAATATTTAGGTCACTGACAGGCAGTCCAATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006900 GO:0006996 GO:0008150 GO:0009987 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031984 GO:0033036 GO:0034204 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045332 GO:0048193 GO:0048194 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase Hydrolase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1261

Amino Acids

142.79

Weight (kDa)

6.71

Isoelectric Point (pI)

38.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhoLip_ATPase_N	PF16209	47 - 113	1.8e-22	Phospholipid-translocating ATPase N-terminal
Cation_ATPase	PF13246	555 - 751	1.1e-16	P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C	PF16212	924 - 1183	1.8e-85	Phospholipid-translocating P-type ATPase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0016785)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g11830
malus_domestica	MD10G1238400.v1.1
prunus_persica	Prupe.4G105900_v2.0.a1
pyrus_communis	pycom10g19940
rosa_chinensis	RchiOBHm_Chr5g0019261
rosa_laevigata	RLG00000032432
rosa_multiflora	Rmu_co8517133.1_g000001
rosa_roxburghii	Rroxscaffold_1G00058740
rosa_rugosa	Rorug05G0049000
rosa_samantha	Rh5AG140100 Rh5BG139100 Rh5CG150500 Rh5DG139700
rosa_wichuraiana	Rw5G012460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1588
Acc65I	GGTACC	1 cut(s)	1020
AccB1I	GGYRCC	3 cut(s)	399, 1020, 3260
AccB7I	CCANNNNNTGG	1 cut(s)	3468
AccI	GTMKAC	1 cut(s)	2925
AccII	CGCG	1 cut(s)	986
AciI	CCGC	3 cut(s)	986, 1484, 1568
AcoI	YGGCCR	4 cut(s)	100, 2583, 2682, 2775
AcsI	RAATTY	6 cut(s)	427, 680, 739, 1319, 1436, 1895
AcuI	CTGAAG	4 cut(s)	52, 1089, 2447, 2505
AfaI	GTAC	7 cut(s)	185, 215, 1022, 1874, 2175, 2882, 3374
AfiI	CCNNNNNNNGG	6 cut(s)	735, 1709, 1850, 2428, 2809, 3468
AflIII	ACRYGT	1 cut(s)	1304
AhdI	GACNNNNNGTC	1 cut(s)	3774
AjiI	CACGTC	1 cut(s)	1305
AjnI	CCWGG	1 cut(s)	97
AjuI	GAANNNNNNNTTGG	4 cut(s)	230, 262, 2715, 2747
AleI	CACNNNNGTG	1 cut(s)	3258
Alw21I	GWGCWC	1 cut(s)	1909
Alw26I	GTCTC	4 cut(s)	306, 584, 1147, 2625
AlwNI	CAGNNNCTG	2 cut(s)	112, 1067
Ama87I	CYCGRG	1 cut(s)	1537
AoxI	GGCC	9 cut(s)	100, 687, 1098, 2583, 2682, 2775, 2899, 3079, 3451
ApaI	GGGCCC	1 cut(s)	1102
ApoI	RAATTY	6 cut(s)	427, 680, 739, 1319, 1436, 1895
AseI	ATTAAT	1 cut(s)	1365
Asp700I	GAANNNNTTC	4 cut(s)	531, 1953, 2466, 3218
Asp718I	GGTACC	1 cut(s)	1020
AspLEI	GCGC	1 cut(s)	3400
AspS9I	GGNCC	9 cut(s)	848, 919, 1098, 1099, 1641, 2156, 2566, 3079, 3452
AsuC2I	CCSGG	1 cut(s)	123
AsuHPI	GGTGA	6 cut(s)	876, 1724, 1740, 1958, 2327, 3310
AsuII	TTCGAA	4 cut(s)	800, 2068, 2858, 3416
AvaI	CYCGRG	1 cut(s)	1537
AvaII	GGWCC	5 cut(s)	848, 919, 1641, 2156, 2566
BaeGI	GKGCMC	3 cut(s)	776, 1102, 2838
BalI	TGGCCA	3 cut(s)	102, 2684, 2777
BamHI	GGATCC	2 cut(s)	706, 3333
BanI	GGYRCC	3 cut(s)	399, 1020, 3260
BanII	GRGCYC	2 cut(s)	448, 1102
BarI	GAAGNNNNNNTAC	2 cut(s)	2229, 2261
BbsI	GAAGAC	2 cut(s)	2244, 2448
Bbv12I	GWGCWC	1 cut(s)	1909
BbvCI	CCTCAGC	1 cut(s)	156
BceAI	ACGGC	2 cut(s)	2570, 3148
BciT130I	CCWGG	1 cut(s)	99
BciVI	GTATCC	2 cut(s)	211, 3117
BclI	TGATCA	2 cut(s)	664, 1714
BcnI	CCSGG	1 cut(s)	123
BcoDI	GTCTC	4 cut(s)	306, 584, 1147, 2625
BfaI	CTAG	9 cut(s)	287, 561, 1563, 1892, 2075, 2415, 3149, 3485, 3669
BfmI	CTRYAG	3 cut(s)	1146, 1900, 2611
BfuAI	ACCTGC	1 cut(s)	1588
BfuI	GTATCC	2 cut(s)	211, 3117
BlpI	GCTNAGC	1 cut(s)	171
BmcAI	AGTACT	1 cut(s)	2175
Bme1390I	CCNGG	2 cut(s)	99, 123
Bme18I	GGWCC	5 cut(s)	848, 919, 1641, 2156, 2566
BmeRI	GACNNNNNGTC	1 cut(s)	3774
BmeT110I	CYCGRG	1 cut(s)	1537
BmgBI	CACGTC	1 cut(s)	1305
BmgT120I	GGNCC	9 cut(s)	848, 919, 1098, 1099, 1641, 2156, 2566, 3079, 3452
BmiI	GGNNCC	8 cut(s)	401, 708, 1022, 1100, 2259, 3107, 3262, 3335
BmrFI	CCNGG	2 cut(s)	99, 123
BmrI	ACTGGG	1 cut(s)	122
BmsI	GCATC	8 cut(s)	53, 1394, 2089, 2195, 2491, 3152, 3369, 3667
BmuI	ACTGGG	1 cut(s)	122
BpiI	GAAGAC	2 cut(s)	2244, 2448
BpmI	CTGGAG	1 cut(s)	1362
Bpu10I	CCTNAGC	1 cut(s)	156
Bpu1102I	GCTNAGC	1 cut(s)	171
Bpu14I	TTCGAA	4 cut(s)	800, 2068, 2858, 3416
BpuEI	CTTGAG	3 cut(s)	1814, 3038, 3044
BpuMI	CCSGG	1 cut(s)	123
Bsa29I	ATCGAT	1 cut(s)	1374
BsaBI	GATNNNNATC	5 cut(s)	93, 711, 886, 1041, 1713
BsaI	GGTCTC	1 cut(s)	584
BsaJI	CCNNGG	4 cut(s)	122, 2508, 2679, 3256
BsaXI	ACNNNNNCTCC	2 cut(s)	492, 522
Bsc4I	CCNNNNNNNGG	6 cut(s)	735, 1709, 1850, 2428, 2809, 3468
Bse1I	ACTGG	8 cut(s)	117, 982, 2317, 2569, 2778, 2843, 2966, 3473
Bse3DI	GCAATG	6 cut(s)	154, 1942, 2789, 2889, 3479, 3522
Bse8I	GATNNNNATC	5 cut(s)	93, 711, 886, 1041, 1713
BseBI	CCWGG	1 cut(s)	99
BseCI	ATCGAT	1 cut(s)	1374
BseDI	CCNNGG	4 cut(s)	122, 2508, 2679, 3256
BseJI	GATNNNNATC	5 cut(s)	93, 711, 886, 1041, 1713
BseLI	CCNNNNNNNGG	6 cut(s)	735, 1709, 1850, 2428, 2809, 3468
BseMI	GCAATG	6 cut(s)	154, 1942, 2789, 2889, 3479, 3522
BseMII	CTCAG	6 cut(s)	170, 651, 2127, 2540, 2952, 3651
BseNI	ACTGG	8 cut(s)	117, 982, 2317, 2569, 2778, 2843, 2966, 3473
BseSI	GKGCMC	3 cut(s)	776, 1102, 2838
BsgI	GTGCAG	6 cut(s)	146, 1401, 1563, 1761, 2430, 3441
Bsh1236I	CGCG	1 cut(s)	986
Bsh1285I	CGRYCG	2 cut(s)	1378, 3539
BshFI	GGCC	9 cut(s)	102, 689, 1100, 2585, 2684, 2777, 2901, 3081, 3453
BshNI	GGYRCC	3 cut(s)	399, 1020, 3260
BshVI	ATCGAT	1 cut(s)	1374
BsiEI	CGRYCG	2 cut(s)	1378, 3539
BsiHKAI	GWGCWC	1 cut(s)	1909
BsiHKCI	CYCGRG	1 cut(s)	1537
BsiSI	CCGG	3 cut(s)	122, 3062, 3109
BslFI	GGGAC	2 cut(s)	1309, 3732
BslI	CCNNNNNNNGG	6 cut(s)	735, 1709, 1850, 2428, 2809, 3468
BsmAI	GTCTC	4 cut(s)	306, 584, 1147, 2625
BsmFI	GGGAC	2 cut(s)	1309, 3732
BsmI	GAATGC	2 cut(s)	2270, 2766
BsnI	GGCC	9 cut(s)	102, 689, 1100, 2585, 2684, 2777, 2901, 3081, 3453
Bso31I	GGTCTC	1 cut(s)	584
BsoBI	CYCGRG	1 cut(s)	1537
Bsp119I	TTCGAA	4 cut(s)	800, 2068, 2858, 3416
Bsp120I	GGGCCC	1 cut(s)	1098
Bsp1286I	GDGCHC	5 cut(s)	448, 776, 1102, 1909, 2838
Bsp1407I	TGTACA	1 cut(s)	2880
Bsp1720I	GCTNAGC	1 cut(s)	171
BspACI	CCGC	3 cut(s)	986, 1484, 1568
BspANI	GGCC	9 cut(s)	102, 689, 1100, 2585, 2684, 2777, 2901, 3081, 3453
BspCNI	CTCAG	6 cut(s)	169, 650, 2128, 2541, 2951, 3650
BspDI	ATCGAT	1 cut(s)	1374
BspFNI	CGCG	1 cut(s)	986
BspLI	GGNNCC	8 cut(s)	401, 708, 1022, 1100, 2259, 3107, 3262, 3335
BspMAI	CTGCAG	1 cut(s)	2615
BspMI	ACCTGC	1 cut(s)	1588
BspQI	GCTCTTC	1 cut(s)	3507
BspT104I	TTCGAA	4 cut(s)	800, 2068, 2858, 3416
BspT107I	GGYRCC	3 cut(s)	399, 1020, 3260
BspTNI	GGTCTC	1 cut(s)	584
BsrDI	GCAATG	6 cut(s)	154, 1942, 2789, 2889, 3479, 3522
BsrGI	TGTACA	1 cut(s)	2880
BsrI	ACTGG	8 cut(s)	117, 982, 2317, 2569, 2778, 2843, 2966, 3473
BssECI	CCNNGG	4 cut(s)	122, 2508, 2679, 3256
BssNAI	GTATAC	1 cut(s)	2926
BssT1I	CCWWGG	2 cut(s)	2508, 2679
Bst1107I	GTATAC	1 cut(s)	2926
Bst2UI	CCWGG	1 cut(s)	99
Bst6I	CTCTTC	3 cut(s)	1055, 3350, 3507
BstAPI	GCANNNNNTGC	1 cut(s)	1174
BstAUI	TGTACA	1 cut(s)	2880
BstBI	TTCGAA	4 cut(s)	800, 2068, 2858, 3416
BstC8I	GCNNGC	3 cut(s)	47, 1170, 2766
BstFNI	CGCG	1 cut(s)	986
BstHHI	GCGC	1 cut(s)	3400
BstMAI	GTCTC	4 cut(s)	306, 584, 1147, 2625
BstMCI	CGRYCG	2 cut(s)	1378, 3539
BstMWI	GCNNNNNNNGC	5 cut(s)	1174, 1511, 2510, 2774, 3215
BstNI	CCWGG	1 cut(s)	99
BstNSI	RCATGY	3 cut(s)	1218, 1531, 3279
BstSCI	CCNGG	2 cut(s)	97, 121
BstSFI	CTRYAG	3 cut(s)	1146, 1900, 2611
BstSLI	GKGCMC	3 cut(s)	776, 1102, 2838
BstUI	CGCG	1 cut(s)	986
BstV2I	GAAGAC	2 cut(s)	2244, 2448
BstX2I	RGATCY	2 cut(s)	706, 3333
BstYI	RGATCY	2 cut(s)	706, 3333
BstZ17I	GTATAC	1 cut(s)	2926
Bsu15I	ATCGAT	1 cut(s)	1374
BsuI	GTATCC	2 cut(s)	211, 3117
BsuRI	GGCC	9 cut(s)	102, 689, 1100, 2585, 2684, 2777, 2901, 3081, 3453
BsuTUI	ATCGAT	1 cut(s)	1374
BtrI	CACGTC	1 cut(s)	1305
BtsI	GCAGTG	2 cut(s)	782, 870
BveI	ACCTGC	1 cut(s)	1588
Cac8I	GCNNGC	3 cut(s)	47, 1170, 2766
CaiI	CAGNNNCTG	2 cut(s)	112, 1067
CfoI	GCGC	1 cut(s)	3400
Cfr13I	GGNCC	9 cut(s)	848, 919, 1098, 1099, 1641, 2156, 2566, 3079, 3452
ClaI	ATCGAT	1 cut(s)	1374
Csp6I	GTAC	7 cut(s)	184, 214, 1021, 1873, 2174, 2881, 3373
CspCI	CAANNNNNGTGG	2 cut(s)	3311, 3346
CviQI	GTAC	7 cut(s)	184, 214, 1021, 1873, 2174, 2881, 3373
DraI	TTTAAA	2 cut(s)	1884, 2451
DriI	GACNNNNNGTC	1 cut(s)	3774
EaeI	YGGCCR	4 cut(s)	100, 2583, 2682, 2775
Eam1104I	CTCTTC	3 cut(s)	1055, 3350, 3507
Eam1105I	GACNNNNNGTC	1 cut(s)	3774
EarI	CTCTTC	3 cut(s)	1055, 3350, 3507
EciI	GGCGGA	1 cut(s)	1499
Eco130I	CCWWGG	2 cut(s)	2508, 2679
Eco24I	GRGCYC	2 cut(s)	448, 1102
Eco31I	GGTCTC	1 cut(s)	584
Eco32I	GATATC	1 cut(s)	3306
Eco47I	GGWCC	5 cut(s)	848, 919, 1641, 2156, 2566
Eco57I	CTGAAG	4 cut(s)	52, 1089, 2447, 2505
Eco88I	CYCGRG	1 cut(s)	1537
EcoO109I	RGGNCCY	3 cut(s)	1098, 1099, 2156
EcoRI	GAATTC	3 cut(s)	427, 680, 1895
EcoRII	CCWGG	1 cut(s)	97
EcoRV	GATATC	1 cut(s)	3306
EcoT14I	CCWWGG	2 cut(s)	2508, 2679
EcoT22I	ATGCAT	2 cut(s)	2270, 3281
EcoT38I	GRGCYC	2 cut(s)	448, 1102
ErhI	CCWWGG	2 cut(s)	2508, 2679
FalI	AAGNNNNNCTT	8 cut(s)	633, 665, 1494, 1526, 2663, 2695, 3689, 3721
FaqI	GGGAC	2 cut(s)	1309, 3732
FauNDI	CATATG	3 cut(s)	1351, 3170, 3350
FbaI	TGATCA	2 cut(s)	664, 1714
FblI	GTMKAC	1 cut(s)	2925
FriOI	GRGCYC	2 cut(s)	448, 1102
FspBI	CTAG	9 cut(s)	287, 561, 1563, 1892, 2075, 2415, 3149, 3485, 3669
GlaI	GCGC	1 cut(s)	3399
GsuI	CTGGAG	1 cut(s)	1362
HaeIII	GGCC	9 cut(s)	102, 689, 1100, 2585, 2684, 2777, 2901, 3081, 3453
HapII	CCGG	3 cut(s)	122, 3062, 3109
HhaI	GCGC	1 cut(s)	3400
Hin6I	GCGC	1 cut(s)	3398
HinP1I	GCGC	1 cut(s)	3398
HincII	GTYRAC	2 cut(s)	119, 517
HindII	GTYRAC	2 cut(s)	119, 517
HindIII	AAGCTT	1 cut(s)	1456
HpaI	GTTAAC	1 cut(s)	517
HpaII	CCGG	3 cut(s)	122, 3062, 3109
HphI	GGTGA	6 cut(s)	876, 1724, 1740, 1958, 2327, 3310
Hpy166II	GTNNAC	5 cut(s)	119, 517, 848, 2881, 2926
Hpy8I	GTNNAC	5 cut(s)	119, 517, 848, 2881, 2926
HpyCH4IV	ACGT	1 cut(s)	1304
HpyF10VI	GCNNNNNNNGC	5 cut(s)	1174, 1511, 2510, 2774, 3215
HpySE526I	ACGT	1 cut(s)	1304
HspAI	GCGC	1 cut(s)	3398
KpnI	GGTACC	1 cut(s)	1024
Ksp22I	TGATCA	2 cut(s)	664, 1714
KspAI	GTTAAC	1 cut(s)	517
LguI	GCTCTTC	1 cut(s)	3507
LmnI	GCTCC	6 cut(s)	42, 322, 349, 1069, 1343, 3105
LweI	GCATC	8 cut(s)	53, 1394, 2089, 2195, 2491, 3152, 3369, 3667
MaeI	CTAG	9 cut(s)	287, 561, 1563, 1892, 2075, 2415, 3149, 3485, 3669
MaeII	ACGT	1 cut(s)	1304
MflI	RGATCY	2 cut(s)	706, 3333
MhlI	GDGCHC	5 cut(s)	448, 776, 1102, 1909, 2838
MlsI	TGGCCA	3 cut(s)	102, 2684, 2777
MluNI	TGGCCA	3 cut(s)	102, 2684, 2777
MlyI	GAGTC	4 cut(s)	1421, 1683, 1754, 3027
MmeI	TCCRAC	9 cut(s)	433, 478, 881, 1108, 1266, 1668, 3101, 3361, 3556
Mox20I	TGGCCA	3 cut(s)	102, 2684, 2777
Mph1103I	ATGCAT	2 cut(s)	2270, 3281
MroXI	GAANNNNTTC	4 cut(s)	531, 1953, 2466, 3218
MscI	TGGCCA	3 cut(s)	102, 2684, 2777
MslI	CAYNNNNRTG	2 cut(s)	3258, 3274
Msp20I	TGGCCA	3 cut(s)	102, 2684, 2777
MspA1I	CMGCKG	1 cut(s)	160
MspI	CCGG	3 cut(s)	122, 3062, 3109
MspR9I	CCNGG	2 cut(s)	99, 123
Mva1269I	GAATGC	2 cut(s)	2270, 2766
MvaI	CCWGG	1 cut(s)	99
MvnI	CGCG	1 cut(s)	986
MwoI	GCNNNNNNNGC	5 cut(s)	1174, 1511, 2510, 2774, 3215
NciI	CCSGG	1 cut(s)	123
NdeI	CATATG	3 cut(s)	1351, 3170, 3350
NlaIV	GGNNCC	8 cut(s)	401, 708, 1022, 1100, 2259, 3107, 3262, 3335
NmuCI	GTSAC	7 cut(s)	223, 1688, 1746, 2315, 2780, 3475, 3762
NsiI	ATGCAT	2 cut(s)	2270, 3281
NspI	RCATGY	3 cut(s)	1218, 1531, 3279
NspV	TTCGAA	4 cut(s)	800, 2068, 2858, 3416
OliI	CACNNNNGTG	1 cut(s)	3258
PaeR7I	CTCGAG	1 cut(s)	1537
PciSI	GCTCTTC	1 cut(s)	3507
PctI	GAATGC	2 cut(s)	2270, 2766
PdmI	GAANNNNTTC	4 cut(s)	531, 1953, 2466, 3218
PfeI	GAWTC	9 cut(s)	89, 797, 1276, 1557, 2716, 2742, 2866, 3565, 3685
PflFI	GACNNNGTC	1 cut(s)	923
PflMI	CCANNNNNTGG	1 cut(s)	3468
Ple19I	CGATCG	1 cut(s)	1378
PleI	GAGTC	4 cut(s)	1420, 1682, 1753, 3026
PpsI	GAGTC	4 cut(s)	1420, 1682, 1753, 3026
PpuMI	RGGWCCY	1 cut(s)	2156
PshBI	ATTAAT	1 cut(s)	1365
Psp5II	RGGWCCY	1 cut(s)	2156
Psp6I	CCWGG	1 cut(s)	97
PspGI	CCWGG	1 cut(s)	97
PspN4I	GGNNCC	8 cut(s)	401, 708, 1022, 1100, 2259, 3107, 3262, 3335
PspOMI	GGGCCC	1 cut(s)	1098
PspPI	GGNCC	9 cut(s)	848, 919, 1098, 1099, 1641, 2156, 2566, 3079, 3452
PspPPI	RGGWCCY	1 cut(s)	2156
PspXI	VCTCGAGB	1 cut(s)	1537
PstI	CTGCAG	1 cut(s)	2615
PstNI	CAGNNNCTG	2 cut(s)	112, 1067
PsuI	RGATCY	2 cut(s)	706, 3333
PsyI	GACNNNGTC	1 cut(s)	923
PvuI	CGATCG	1 cut(s)	1378
PvuII	CAGCTG	1 cut(s)	160
RsaI	GTAC	7 cut(s)	185, 215, 1022, 1874, 2175, 2882, 3374
RsaNI	GTAC	7 cut(s)	184, 214, 1021, 1873, 2174, 2881, 3373
RseI	CAYNNNNRTG	2 cut(s)	3258, 3274
SapI	GCTCTTC	1 cut(s)	3507
Sau96I	GGNCC	9 cut(s)	848, 919, 1098, 1099, 1641, 2156, 2566, 3079, 3452
ScaI	AGTACT	1 cut(s)	2175
SchI	GAGTC	4 cut(s)	1421, 1683, 1754, 3027
ScrFI	CCNGG	2 cut(s)	99, 123
SduI	GDGCHC	5 cut(s)	448, 776, 1102, 1909, 2838
SfaNI	GCATC	8 cut(s)	53, 1394, 2089, 2195, 2491, 3152, 3369, 3667
SfcI	CTRYAG	3 cut(s)	1146, 1900, 2611
Sfr274I	CTCGAG	1 cut(s)	1537
SfuI	TTCGAA	4 cut(s)	800, 2068, 2858, 3416
SinI	GGWCC	5 cut(s)	848, 919, 1641, 2156, 2566
SlaI	CTCGAG	1 cut(s)	1537
SmiMI	CAYNNNNRTG	2 cut(s)	3258, 3274
SmlI	CTYRAG	4 cut(s)	1537, 1829, 3023, 3053
SmoI	CTYRAG	4 cut(s)	1537, 1829, 3023, 3053
SsiI	CCGC	3 cut(s)	986, 1484, 1568
SspI	AATATT	4 cut(s)	530, 2130, 2341, 3756
SspMI	CTAG	9 cut(s)	287, 561, 1563, 1892, 2075, 2415, 3149, 3485, 3669
StyD4I	CCNGG	2 cut(s)	97, 121
StyI	CCWWGG	2 cut(s)	2508, 2679
TaiI	ACGT	1 cut(s)	1307
TatI	WGTACW	4 cut(s)	213, 1872, 2173, 2880
TfiI	GAWTC	9 cut(s)	89, 797, 1276, 1557, 2716, 2742, 2866, 3565, 3685
TseFI	GTSAC	7 cut(s)	223, 1688, 1746, 2315, 2780, 3475, 3762
Tsp45I	GTSAC	7 cut(s)	223, 1688, 1746, 2315, 2780, 3475, 3762
TspGWI	ACGGA	3 cut(s)	1298, 1807, 1833
Tth111I	GACNNNGTC	1 cut(s)	923
Van91I	CCANNNNNTGG	1 cut(s)	3468
VpaK11BI	GGWCC	5 cut(s)	848, 919, 1641, 2156, 2566
VspI	ATTAAT	1 cut(s)	1365
XapI	RAATTY	6 cut(s)	427, 680, 739, 1319, 1436, 1895
XbaI	TCTAGA	1 cut(s)	1891
XceI	RCATGY	3 cut(s)	1218, 1531, 3279
XcmI	CCANNNNNNNNNTGG	1 cut(s)	652
XhoI	CTCGAG	1 cut(s)	1537
XmiI	GTMKAC	1 cut(s)	2925
XmnI	GAANNNNTTC	4 cut(s)	531, 1953, 2466, 3218
XspI	CTAG	9 cut(s)	287, 561, 1563, 1892, 2075, 2415, 3149, 3485, 3669
ZrmI	AGTACT	1 cut(s)	2175
Zsp2I	ATGCAT	2 cut(s)	2270, 3281

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.