Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0016785

Physical Location & Seq

Genomic Coordinates Reverse (-)

14626999 .. 14632321

Gene Structure 5323 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G012460.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3720 bp

ATGTCAGGGTCATCTGGAAGAAGAAAGAGGAAGATAAGATGGAGCAAGCTGTATTCCTATGGATGCTTCAGACCACCCCACACTCTTGATTCTGATCCTGGCCAACAGCTACTGGGTCAACCGGGGTTTTCCCGGGTGGTTTTCTGCAATGAACCTCAGCTGCACAAGGCTAAGCCTTACAAGTACCCCAAGAACTATGTATCCACAACCAAGTACAACATTGTCACTTTTCTTCCCAAAGCTCTTTTCGAGCAGTTTCGCAGAGTTGCCAATCTTTATTTCCTTCTAGCAGCAGTTTTGTCTCTCAAGTCTTTCGCTCCCTTTAATCCAATAAGTTTGATTGCTCCTTTGGTGTTTGTTGTGGGAGTTAGCATGATTAAAGAGGCTGTCGAGGATTGGCACCGATTCTTGCAGGATTTGAATGTGAATTCAAGAACTGTTAGGGCTCATGTTGGAGGTGGCTTATTTGTTGAAAAGACATGGCAAGGACTTTGTGTTGGAGATGTTGTTAAAGTTAACAAGAATGAATATTTTCCAAGTGATCTTCTCTTGCTATCTTCTAGCTTTGAGGATGGCATATGTTATGTCGAGACCATGAACCTCGATGGAGAAACTAATCTGAAAGTTAAGAGAAGCTCAGAAGCCACACTTGACTTGGCAAATGATCAAGCATTTAGTGAATTCAAGGCCACAATTCGCTGTGAGGATCCAAATCCTCATCTTTACACCTTTGTTGGGAATTTAGAGTTGAACTGTGTAACACATCCCTTGTGCCCTGCCACACTGCTTCTAAGAGATTCGAAACTTAGAAATACTGATTACATTTATGGGGTTGTGATCTTTAGTGGACCTGACACAAAAGCAGTGAGGAACTCAACAAGATCACCATCCAAGCGTAGTCGGATTGAAAGAAAGATGGATCTTGTCATTTACCTTCTCTTTTCAATGCTTCTTCTCATTTCACTCATCACTTCAACTGGTTTCACGGTGATTCTGAAATCACAAATGGCCAAGTGGTGGTACCTTTCTCTGCAAGATGATGATCAACTATTTAAACCATTAAAGCCTGGAGTATCTGGTTTCCTACAATTTGTAAGGGCCCTTATTTTGTATGGCTACTTGATCCCCATTTCTCTCTATGTCTCTATAGAGGTTGTCAAAGTTTTGCAAGCCATGCTCATTAACAAGGATATAGAGTTGTATGATGAAGTTACATGCAAGTCAGTTCAAACCAGAACATCCAACTTGAATGAAGAACTTGGTCAAGTAGAGATGATTCTGACGGATAAAACGGGGACTTTGACGTGTAATCAGATGGAATTTAGGAAATGCTCTATTGCTGGAGCTTCATATGGGGGTAACATTAATAATATCGATCGTGCAGCATCTAAAAGGATGAATGTTGAGGTTGAGTCGTATCGTTTTAGTATGGATGAATTTGAAACACAATGCCAAAGCTTTGAAATGTTTGACTTTTCAGTGGCGGATGGTAGCACTGAAAAAGCTGCTCTTGCTGGACAGAAGCACATGCAGAACTCGAGTGCAGACTGTTCCAGAATCTCTAGTGCGGAGGGAGAACCTGCCATCAAAGGTTTCAACTTTTGGGATGATAGGCTTCTGAACAGAAAGTGGGTTTATAGGTCCAACTTATTTGATGTAACAATGTTCTTTAGAGTCATGGCTCTATGTCACACAGGCATACCCGTTGATGGTGATCAGCCTCATAAGCTAAAGTATGAGGCAGAGTCACCAGAAGAAGTATCTTTTCTAATTGCTGCACAAGAGTTTGGATTCCAATTTTTTCAAAGAAGTCAGTCCGTTATGTATCTCAAGGAGTTTGATCCGTCCTCTGGAAAGATGGTTGAGAGGAAGTACAAACTTTTAAACCTTCTAGAATTCTGTAGTGCTCGAAAGAGGATGTCAGTTATTGTAAGCAATGAAGATGGTGAAATCTTTCTTCTCTGCAAAGGGGCAGATAACATTATCTTTGATAGACTTGCAGACAATGGTAGGTCTTACCAAGAGGCTACAACTCTACACCTTTCAGATTATGCAGAAGATGGATTTCGAACTCTAGCATTTGCTTATCGAAAACTTGATGCTGTCGAGTATGAAAAATGGAACTCAATATTTACCGAGGCTAAGACCACAATAGGTCCTGAAAGAGAAGAAGTACTAATAGAAGCATCTGAAATGATTGAAAAGGATTTAATTCTATTAGGGGTTGCTGCTATTGAAGACAAGTTACAAAAAGGGGTTCCAGAATGCATAGATAAACTTGCACAAGCAGGAATGAAAATATGGCTGCTCACTGGTGACAAGAAAGAAACTGCAATAAATATTGGATTTTCTTGCAGCTTACTTCGGGAGGATATGAAACAGTTTCATCTAATTTTGGGCAAAGAAAGTGCAGCTAGGAACCGACTGAAGGAGATGAAGGAAGACATTTTAAACCAACTTGAAGGCTTTCAGAAAGTGAAGTCTGAAGAAAGCAACAAAGATGCACCCTTGGCTTTGATAGTAGATGGAAAAGCTCTTGAAATTGCTCTGAGAAGTGATGTGAAGGACCAGTTCTTACCGTTGGCCGTTGATTGTGCTTCTGTTATATGCTGCAGGGTGTCTCCAAAACAAAAGGCTCTGATTACTCGATTGGTAAAGGAACATACGGGCAAGACAACCTTGGCTATTGGAGATGGGGCAAATGATGTTGGCATGATTCAAGAAGCTGATATTGGAGTAGGAATCAGTGGCATGGAAGGAATGCAGGCAGTCATGGCTAGTGACATCTCATTGCCTCAATTCCGATTTTTGGGGAGATTACTTATTGTTCATGGGCACTGGTGTTACAAGAGGATTTCGAAAATGATTCTCTACTTCGTGTACAAAAACATTGCATTTGGCCTCACACTGTTCTACTATGAAGTATACACAAGTTTCTCAGGGGAGGTCTTATATGATGACTGGTATATGGCACTATTCAATGTAATTTTGACATCTTTGCCTGTTATATCATTGGGAGTCCTTGAGCAAGATGTTTCATCCGAAGTGTGCCTCAAGTTTCCGGCTCTCTACCAACAGGGCCAAAAGAACATATACTTCACTTGGAGCCGCATCATCGGTTGGATACTCAACGGCATTGTAGCATCTCTAGTCATCTTCCTTGCAAACATATGCACACTTTCTACCTTCAACAAAGATGGTAGTTTTGCAGAGAAAGCCTTCGCCAAAGACGGTAATGTTGCAGACATCACACACCTCGGTGCCATGACATACACATGCATAATCTGGACAGTCAATTGCCAGATATCCCTCATCATCACCCACTTCACTTGGATCCAACATCTCTTCATATGGGGCAGCATCATCATCTGGTACATCTTCCTGTTCATCTACGGTGCGCTACCTCCTGACATTTCGAAAAGAGGCTTTCGTGTTCTTGTAGAAGCTCTTGGCCCTGCACCCATTTACTGGCTTGCCACATTGCTAGTTGTGGTTGTTGCTCTTCTTCCTTATTTCATCCACATTGCGATCCAACGGTTGTTTTATCCTTTGGATGACCATGTGATTCAAGAAATGAAGTATTGCAGAAAAGATATTGGGAAAAATAATCTAATTTGGGAGAGGGAACAGAACAACTCAGTGATGATGACCCAGATAGGATTTTCGGCTAGAGTTGATGCAAGGATTCGGATTATGAAGGAAAACTTGAACCAGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006900 GO:0006996 GO:0008150 GO:0009987 GO:0010876 GO:0012505 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031984 GO:0033036 GO:0034204 GO:0042623 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045332 GO:0048193 GO:0048194 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase Hydrolase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1239

Amino Acids

140.56

Weight (kDa)

7.01

Isoelectric Point (pI)

37.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhoLip_ATPase_N	PF16209	47 - 113	1.2e-22	Phospholipid-translocating ATPase N-terminal
Cation_ATPase	PF13246	555 - 751	6.7e-17	P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C	PF16212	924 - 1183	4.2e-85	Phospholipid-translocating P-type ATPase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0016785)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g11830
malus_domestica	MD10G1238400.v1.1
prunus_persica	Prupe.4G105900_v2.0.a1
pyrus_communis	pycom10g19940
rosa_chinensis	RchiOBHm_Chr5g0019261
rosa_laevigata	RLG00000032432
rosa_multiflora	Rmu_co8517133.1_g000001
rosa_roxburghii	Rroxscaffold_1G00058740
rosa_rugosa	Rorug05G0049000
rosa_samantha	Rh5AG140100 Rh5BG139100 Rh5CG150500 Rh5DG139700
rosa_wichuraiana	Rw5G012460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1588
Acc65I	GGTACC	1 cut(s)	1020
AccB1I	GGYRCC	3 cut(s)	399, 1020, 3260
AccB7I	CCANNNNNTGG	2 cut(s)	1017, 3468
AccI	GTMKAC	1 cut(s)	2925
AciI	CCGC	3 cut(s)	1484, 1568, 3109
AclWI	GGATC	9 cut(s)	89, 701, 714, 927, 1117, 1835, 3328, 3341, 3523
AcoI	YGGCCR	3 cut(s)	100, 1008, 2583
AcsI	RAATTY	6 cut(s)	427, 680, 739, 1319, 1436, 1895
AcuI	CTGAAG	3 cut(s)	52, 2447, 2505
AfaI	GTAC	7 cut(s)	185, 215, 1022, 1874, 2175, 2882, 3374
AfiI	CCNNNNNNNGG	7 cut(s)	735, 1017, 1709, 1850, 2428, 2809, 3468
AflIII	ACRYGT	1 cut(s)	1304
AjiI	CACGTC	1 cut(s)	1305
AjnI	CCWGG	2 cut(s)	97, 1066
AjuI	GAANNNNNNNTTGG	6 cut(s)	230, 262, 263, 295, 2715, 2747
AleI	CACNNNNGTG	1 cut(s)	3258
AloI	GAACNNNNNNTCC	2 cut(s)	185, 217
Alw21I	GWGCWC	1 cut(s)	1909
Alw26I	GTCTC	4 cut(s)	306, 584, 1147, 2625
AlwI	GGATC	9 cut(s)	89, 701, 714, 927, 1117, 1835, 3328, 3341, 3523
AlwNI	CAGNNNCTG	1 cut(s)	112
Ama87I	CYCGRG	2 cut(s)	132, 1537
AoxI	GGCC	8 cut(s)	100, 687, 1008, 1098, 2583, 2899, 3079, 3451
ApaI	GGGCCC	1 cut(s)	1102
ApoI	RAATTY	6 cut(s)	427, 680, 739, 1319, 1436, 1895
AseI	ATTAAT	1 cut(s)	1365
Asp700I	GAANNNNTTC	4 cut(s)	531, 1953, 2466, 3218
Asp718I	GGTACC	1 cut(s)	1020
AspLEI	GCGC	1 cut(s)	3400
AspS9I	GGNCC	8 cut(s)	848, 1098, 1099, 1641, 2156, 2566, 3079, 3452
AsuC2I	CCSGG	3 cut(s)	123, 133, 134
AsuHPI	GGTGA	7 cut(s)	876, 1000, 1724, 1740, 1958, 2327, 3310
AsuII	TTCGAA	4 cut(s)	800, 2068, 2858, 3416
AvaI	CYCGRG	2 cut(s)	132, 1537
AvaII	GGWCC	4 cut(s)	848, 1641, 2156, 2566
BaeGI	GKGCMC	3 cut(s)	776, 1102, 2838
BalI	TGGCCA	2 cut(s)	102, 1010
BamHI	GGATCC	2 cut(s)	706, 3333
BanI	GGYRCC	3 cut(s)	399, 1020, 3260
BanII	GRGCYC	2 cut(s)	448, 1102
BarI	GAAGNNNNNNTAC	2 cut(s)	2229, 2261
BbsI	GAAGAC	2 cut(s)	2244, 2448
Bbv12I	GWGCWC	1 cut(s)	1909
BbvCI	CCTCAGC	1 cut(s)	156
BceAI	ACGGC	2 cut(s)	2570, 3148
BciT130I	CCWGG	2 cut(s)	99, 1068
BciVI	GTATCC	2 cut(s)	211, 3117
BclI	TGATCA	3 cut(s)	664, 1042, 1714
BcnI	CCSGG	3 cut(s)	123, 133, 134
BcoDI	GTCTC	4 cut(s)	306, 584, 1147, 2625
BfmI	CTRYAG	3 cut(s)	1146, 1900, 2611
BfuAI	ACCTGC	1 cut(s)	1588
BfuI	GTATCC	2 cut(s)	211, 3117
BlpI	GCTNAGC	1 cut(s)	171
BmcAI	AGTACT	1 cut(s)	2175
Bme1390I	CCNGG	5 cut(s)	99, 123, 133, 134, 1068
Bme18I	GGWCC	4 cut(s)	848, 1641, 2156, 2566
BmeT110I	CYCGRG	2 cut(s)	132, 1537
BmgBI	CACGTC	1 cut(s)	1305
BmgT120I	GGNCC	8 cut(s)	848, 1098, 1099, 1641, 2156, 2566, 3079, 3452
BmiI	GGNNCC	9 cut(s)	401, 708, 1022, 1100, 2259, 2420, 3107, 3262, 3335
BmrFI	CCNGG	5 cut(s)	99, 123, 133, 134, 1068
BmrI	ACTGGG	1 cut(s)	122
BmsI	GCATC	9 cut(s)	53, 1394, 2089, 2195, 2491, 3120, 3152, 3369, 3667
BmuI	ACTGGG	1 cut(s)	122
BpiI	GAAGAC	2 cut(s)	2244, 2448
BpmI	CTGGAG	2 cut(s)	1089, 1362
Bpu10I	CCTNAGC	1 cut(s)	156
Bpu1102I	GCTNAGC	1 cut(s)	171
Bpu14I	TTCGAA	4 cut(s)	800, 2068, 2858, 3416
BpuEI	CTTGAG	4 cut(s)	290, 1814, 3038, 3044
BpuMI	CCSGG	3 cut(s)	123, 133, 134
Bsa29I	ATCGAT	1 cut(s)	1374
BsaBI	GATNNNNATC	5 cut(s)	93, 711, 886, 1041, 1713
BsaI	GGTCTC	1 cut(s)	584
BsaJI	CCNNGG	6 cut(s)	122, 132, 2136, 2508, 2679, 3256
BsaXI	ACNNNNNCTCC	2 cut(s)	492, 522
Bsc4I	CCNNNNNNNGG	7 cut(s)	735, 1017, 1709, 1850, 2428, 2809, 3468
Bse1I	ACTGG	7 cut(s)	117, 982, 2317, 2569, 2843, 2966, 3473
Bse3DI	GCAATG	6 cut(s)	154, 1942, 2789, 2889, 3479, 3522
Bse8I	GATNNNNATC	5 cut(s)	93, 711, 886, 1041, 1713
BseBI	CCWGG	2 cut(s)	99, 1068
BseCI	ATCGAT	1 cut(s)	1374
BseDI	CCNNGG	6 cut(s)	122, 132, 2136, 2508, 2679, 3256
BseJI	GATNNNNATC	5 cut(s)	93, 711, 886, 1041, 1713
BseLI	CCNNNNNNNGG	7 cut(s)	735, 1017, 1709, 1850, 2428, 2809, 3468
BseMI	GCAATG	6 cut(s)	154, 1942, 2789, 2889, 3479, 3522
BseMII	CTCAG	5 cut(s)	170, 651, 2540, 2952, 3651
BseNI	ACTGG	7 cut(s)	117, 982, 2317, 2569, 2843, 2966, 3473
BseSI	GKGCMC	3 cut(s)	776, 1102, 2838
BsgI	GTGCAG	6 cut(s)	146, 1401, 1563, 1761, 2430, 3441
Bsh1285I	CGRYCG	1 cut(s)	1378
BshFI	GGCC	8 cut(s)	102, 689, 1010, 1100, 2585, 2901, 3081, 3453
BshNI	GGYRCC	3 cut(s)	399, 1020, 3260
BshVI	ATCGAT	1 cut(s)	1374
BsiEI	CGRYCG	1 cut(s)	1378
BsiHKAI	GWGCWC	1 cut(s)	1909
BsiHKCI	CYCGRG	2 cut(s)	132, 1537
BsiSI	CCGG	3 cut(s)	122, 133, 3062
BslFI	GGGAC	1 cut(s)	1309
BslI	CCNNNNNNNGG	7 cut(s)	735, 1017, 1709, 1850, 2428, 2809, 3468
BsmAI	GTCTC	4 cut(s)	306, 584, 1147, 2625
BsmFI	GGGAC	1 cut(s)	1309
BsmI	GAATGC	2 cut(s)	2270, 2766
BsnI	GGCC	8 cut(s)	102, 689, 1010, 1100, 2585, 2901, 3081, 3453
Bso31I	GGTCTC	1 cut(s)	584
BsoBI	CYCGRG	2 cut(s)	132, 1537
Bsp119I	TTCGAA	4 cut(s)	800, 2068, 2858, 3416
Bsp120I	GGGCCC	1 cut(s)	1098
Bsp1286I	GDGCHC	5 cut(s)	448, 776, 1102, 1909, 2838
Bsp1407I	TGTACA	1 cut(s)	2880
Bsp1720I	GCTNAGC	1 cut(s)	171
BspACI	CCGC	3 cut(s)	1484, 1568, 3109
BspANI	GGCC	8 cut(s)	102, 689, 1010, 1100, 2585, 2901, 3081, 3453
BspCNI	CTCAG	5 cut(s)	169, 650, 2541, 2951, 3650
BspDI	ATCGAT	1 cut(s)	1374
BspLI	GGNNCC	9 cut(s)	401, 708, 1022, 1100, 2259, 2420, 3107, 3262, 3335
BspMAI	CTGCAG	1 cut(s)	2615
BspMI	ACCTGC	1 cut(s)	1588
BspPI	GGATC	9 cut(s)	89, 701, 714, 927, 1117, 1835, 3328, 3341, 3523
BspQI	GCTCTTC	1 cut(s)	3507
BspT104I	TTCGAA	4 cut(s)	800, 2068, 2858, 3416
BspT107I	GGYRCC	3 cut(s)	399, 1020, 3260
BspTNI	GGTCTC	1 cut(s)	584
BsrDI	GCAATG	6 cut(s)	154, 1942, 2789, 2889, 3479, 3522
BsrGI	TGTACA	1 cut(s)	2880
BsrI	ACTGG	7 cut(s)	117, 982, 2317, 2569, 2843, 2966, 3473
BssECI	CCNNGG	6 cut(s)	122, 132, 2136, 2508, 2679, 3256
BssNAI	GTATAC	1 cut(s)	2926
BssT1I	CCWWGG	2 cut(s)	2508, 2679
Bst1107I	GTATAC	1 cut(s)	2926
Bst2UI	CCWGG	2 cut(s)	99, 1068
Bst6I	CTCTTC	2 cut(s)	3350, 3507
BstAPI	GCANNNNNTGC	1 cut(s)	1174
BstAUI	TGTACA	1 cut(s)	2880
BstBI	TTCGAA	4 cut(s)	800, 2068, 2858, 3416
BstC8I	GCNNGC	4 cut(s)	47, 1170, 2766, 3474
BstDEI	CTNAG	9 cut(s)	156, 171, 637, 791, 806, 2142, 2549, 2938, 3637
BstHHI	GCGC	1 cut(s)	3400
BstMAI	GTCTC	4 cut(s)	306, 584, 1147, 2625
BstMCI	CGRYCG	1 cut(s)	1378
BstMWI	GCNNNNNNNGC	6 cut(s)	1174, 1511, 1726, 2510, 2774, 3215
BstNI	CCWGG	2 cut(s)	99, 1068
BstNSI	RCATGY	3 cut(s)	1218, 1531, 3279
BstSCI	CCNGG	5 cut(s)	97, 121, 131, 132, 1066
BstSFI	CTRYAG	3 cut(s)	1146, 1900, 2611
BstSLI	GKGCMC	3 cut(s)	776, 1102, 2838
BstV2I	GAAGAC	2 cut(s)	2244, 2448
BstX2I	RGATCY	3 cut(s)	706, 919, 3333
BstYI	RGATCY	3 cut(s)	706, 919, 3333
BstZ17I	GTATAC	1 cut(s)	2926
Bsu15I	ATCGAT	1 cut(s)	1374
BsuI	GTATCC	2 cut(s)	211, 3117
BsuRI	GGCC	8 cut(s)	102, 689, 1010, 1100, 2585, 2901, 3081, 3453
BsuTUI	ATCGAT	1 cut(s)	1374
BtrI	CACGTC	1 cut(s)	1305
BtsI	GCAGTG	2 cut(s)	782, 870
BtsIMutI	CAGTG	9 cut(s)	782, 870, 1485, 1494, 2310, 2752, 2836, 2906, 3645
BveI	ACCTGC	1 cut(s)	1588
Cac8I	GCNNGC	4 cut(s)	47, 1170, 2766, 3474
CaiI	CAGNNNCTG	1 cut(s)	112
CfoI	GCGC	1 cut(s)	3400
Cfr13I	GGNCC	8 cut(s)	848, 1098, 1099, 1641, 2156, 2566, 3079, 3452
Cfr9I	CCCGGG	1 cut(s)	132
ClaI	ATCGAT	1 cut(s)	1374
Csp6I	GTAC	7 cut(s)	184, 214, 1021, 1873, 2174, 2881, 3373
CspCI	CAANNNNNGTGG	2 cut(s)	3311, 3346
CviQI	GTAC	7 cut(s)	184, 214, 1021, 1873, 2174, 2881, 3373
DdeI	CTNAG	9 cut(s)	156, 171, 637, 791, 806, 2142, 2549, 2938, 3637
DraI	TTTAAA	3 cut(s)	1054, 1884, 2451
EaeI	YGGCCR	3 cut(s)	100, 1008, 2583
Eam1104I	CTCTTC	2 cut(s)	3350, 3507
EarI	CTCTTC	2 cut(s)	3350, 3507
EciI	GGCGGA	1 cut(s)	1499
Eco130I	CCWWGG	2 cut(s)	2508, 2679
Eco24I	GRGCYC	2 cut(s)	448, 1102
Eco31I	GGTCTC	1 cut(s)	584
Eco32I	GATATC	1 cut(s)	3306
Eco47I	GGWCC	4 cut(s)	848, 1641, 2156, 2566
Eco57I	CTGAAG	3 cut(s)	52, 2447, 2505
Eco88I	CYCGRG	2 cut(s)	132, 1537
EcoO109I	RGGNCCY	3 cut(s)	1098, 1099, 2156
EcoRI	GAATTC	3 cut(s)	427, 680, 1895
EcoRII	CCWGG	2 cut(s)	97, 1066
EcoRV	GATATC	1 cut(s)	3306
EcoT14I	CCWWGG	2 cut(s)	2508, 2679
EcoT22I	ATGCAT	2 cut(s)	2270, 3281
EcoT38I	GRGCYC	2 cut(s)	448, 1102
ErhI	CCWWGG	2 cut(s)	2508, 2679
FalI	AAGNNNNNCTT	7 cut(s)	633, 665, 1494, 1526, 2663, 2695, 3689
FaqI	GGGAC	1 cut(s)	1309
FauNDI	CATATG	4 cut(s)	578, 1351, 3170, 3350
FbaI	TGATCA	3 cut(s)	664, 1042, 1714
FblI	GTMKAC	1 cut(s)	2925
FriOI	GRGCYC	2 cut(s)	448, 1102
GlaI	GCGC	1 cut(s)	3399
GsuI	CTGGAG	2 cut(s)	1089, 1362
HaeIII	GGCC	8 cut(s)	102, 689, 1010, 1100, 2585, 2901, 3081, 3453
HapII	CCGG	3 cut(s)	122, 133, 3062
HhaI	GCGC	1 cut(s)	3400
Hin6I	GCGC	1 cut(s)	3398
HinP1I	GCGC	1 cut(s)	3398
HincII	GTYRAC	2 cut(s)	119, 517
HindII	GTYRAC	2 cut(s)	119, 517
HindIII	AAGCTT	1 cut(s)	1456
HpaI	GTTAAC	1 cut(s)	517
HpaII	CCGG	3 cut(s)	122, 133, 3062
HphI	GGTGA	7 cut(s)	876, 1000, 1724, 1740, 1958, 2327, 3310
Hpy166II	GTNNAC	5 cut(s)	119, 517, 848, 2881, 2926
Hpy8I	GTNNAC	5 cut(s)	119, 517, 848, 2881, 2926
HpyCH4IV	ACGT	1 cut(s)	1304
HpyF10VI	GCNNNNNNNGC	6 cut(s)	1174, 1511, 1726, 2510, 2774, 3215
HpyF3I	CTNAG	9 cut(s)	156, 171, 637, 791, 806, 2142, 2549, 2938, 3637
HpySE526I	ACGT	1 cut(s)	1304
HspAI	GCGC	1 cut(s)	3398
KpnI	GGTACC	1 cut(s)	1024
Ksp22I	TGATCA	3 cut(s)	664, 1042, 1714
KspAI	GTTAAC	1 cut(s)	517
LguI	GCTCTTC	1 cut(s)	3507
LmnI	GCTCC	5 cut(s)	42, 322, 349, 1343, 3105
LweI	GCATC	9 cut(s)	53, 1394, 2089, 2195, 2491, 3120, 3152, 3369, 3667
MaeII	ACGT	1 cut(s)	1304
MfeI	CAATTG	1 cut(s)	3295
MflI	RGATCY	3 cut(s)	706, 919, 3333
MhlI	GDGCHC	5 cut(s)	448, 776, 1102, 1909, 2838
MlsI	TGGCCA	2 cut(s)	102, 1010
MluNI	TGGCCA	2 cut(s)	102, 1010
MlyI	GAGTC	4 cut(s)	1421, 1683, 1754, 3027
MmeI	TCCRAC	8 cut(s)	433, 478, 881, 1266, 1668, 3101, 3361, 3556
Mox20I	TGGCCA	2 cut(s)	102, 1010
Mph1103I	ATGCAT	2 cut(s)	2270, 3281
MroXI	GAANNNNTTC	4 cut(s)	531, 1953, 2466, 3218
MscI	TGGCCA	2 cut(s)	102, 1010
MslI	CAYNNNNRTG	2 cut(s)	3258, 3274
Msp20I	TGGCCA	2 cut(s)	102, 1010
MspA1I	CMGCKG	1 cut(s)	160
MspI	CCGG	3 cut(s)	122, 133, 3062
MspR9I	CCNGG	5 cut(s)	99, 123, 133, 134, 1068
MunI	CAATTG	1 cut(s)	3295
Mva1269I	GAATGC	2 cut(s)	2270, 2766
MvaI	CCWGG	2 cut(s)	99, 1068
MwoI	GCNNNNNNNGC	6 cut(s)	1174, 1511, 1726, 2510, 2774, 3215
NciI	CCSGG	3 cut(s)	123, 133, 134
NdeI	CATATG	4 cut(s)	578, 1351, 3170, 3350
NlaIV	GGNNCC	9 cut(s)	401, 708, 1022, 1100, 2259, 2420, 3107, 3262, 3335
NmuCI	GTSAC	5 cut(s)	223, 1688, 1746, 2315, 2780
NsiI	ATGCAT	2 cut(s)	2270, 3281
NspI	RCATGY	3 cut(s)	1218, 1531, 3279
NspV	TTCGAA	4 cut(s)	800, 2068, 2858, 3416
OliI	CACNNNNGTG	1 cut(s)	3258
PaeR7I	CTCGAG	1 cut(s)	1537
PciSI	GCTCTTC	1 cut(s)	3507
PctI	GAATGC	2 cut(s)	2270, 2766
PdmI	GAANNNNTTC	4 cut(s)	531, 1953, 2466, 3218
PflMI	CCANNNNNTGG	2 cut(s)	1017, 3468
Ple19I	CGATCG	1 cut(s)	1378
PleI	GAGTC	4 cut(s)	1420, 1682, 1753, 3026
PpsI	GAGTC	4 cut(s)	1420, 1682, 1753, 3026
PpuMI	RGGWCCY	1 cut(s)	2156
PshBI	ATTAAT	1 cut(s)	1365
Psp5II	RGGWCCY	1 cut(s)	2156
Psp6I	CCWGG	2 cut(s)	97, 1066
PspGI	CCWGG	2 cut(s)	97, 1066
PspN4I	GGNNCC	9 cut(s)	401, 708, 1022, 1100, 2259, 2420, 3107, 3262, 3335
PspOMI	GGGCCC	1 cut(s)	1098
PspPI	GGNCC	8 cut(s)	848, 1098, 1099, 1641, 2156, 2566, 3079, 3452
PspPPI	RGGWCCY	1 cut(s)	2156
PspXI	VCTCGAGB	1 cut(s)	1537
PstI	CTGCAG	1 cut(s)	2615
PstNI	CAGNNNCTG	1 cut(s)	112
PsuI	RGATCY	3 cut(s)	706, 919, 3333
PvuI	CGATCG	1 cut(s)	1378
PvuII	CAGCTG	1 cut(s)	160
RsaI	GTAC	7 cut(s)	185, 215, 1022, 1874, 2175, 2882, 3374
RsaNI	GTAC	7 cut(s)	184, 214, 1021, 1873, 2174, 2881, 3373
RseI	CAYNNNNRTG	2 cut(s)	3258, 3274
SapI	GCTCTTC	1 cut(s)	3507
Sau96I	GGNCC	8 cut(s)	848, 1098, 1099, 1641, 2156, 2566, 3079, 3452
ScaI	AGTACT	1 cut(s)	2175
SchI	GAGTC	4 cut(s)	1421, 1683, 1754, 3027
ScrFI	CCNGG	5 cut(s)	99, 123, 133, 134, 1068
SduI	GDGCHC	5 cut(s)	448, 776, 1102, 1909, 2838
SfaNI	GCATC	9 cut(s)	53, 1394, 2089, 2195, 2491, 3120, 3152, 3369, 3667
SfcI	CTRYAG	3 cut(s)	1146, 1900, 2611
Sfr274I	CTCGAG	1 cut(s)	1537
SfuI	TTCGAA	4 cut(s)	800, 2068, 2858, 3416
SinI	GGWCC	4 cut(s)	848, 1641, 2156, 2566
SlaI	CTCGAG	1 cut(s)	1537
SmaI	CCCGGG	1 cut(s)	134
SmiMI	CAYNNNNRTG	2 cut(s)	3258, 3274
SmlI	CTYRAG	5 cut(s)	305, 1537, 1829, 3023, 3053
SmoI	CTYRAG	5 cut(s)	305, 1537, 1829, 3023, 3053
SsiI	CCGC	3 cut(s)	1484, 1568, 3109
SspI	AATATT	3 cut(s)	530, 2130, 2341
StyD4I	CCNGG	5 cut(s)	97, 121, 131, 132, 1066
StyI	CCWWGG	2 cut(s)	2508, 2679
TaiI	ACGT	1 cut(s)	1307
TatI	WGTACW	4 cut(s)	213, 1872, 2173, 2880
TauI	GCSGC	1 cut(s)	3111
TscAI	CASTG	9 cut(s)	789, 870, 1485, 1501, 2317, 2752, 2843, 2913, 3645
TseFI	GTSAC	5 cut(s)	223, 1688, 1746, 2315, 2780
Tsp45I	GTSAC	5 cut(s)	223, 1688, 1746, 2315, 2780
TspGWI	ACGGA	3 cut(s)	1298, 1807, 1833
TspMI	CCCGGG	1 cut(s)	132
TspRI	CASTG	9 cut(s)	789, 870, 1485, 1501, 2317, 2752, 2843, 2913, 3645
Van91I	CCANNNNNTGG	2 cut(s)	1017, 3468
VpaK11BI	GGWCC	4 cut(s)	848, 1641, 2156, 2566
VspI	ATTAAT	1 cut(s)	1365
XapI	RAATTY	6 cut(s)	427, 680, 739, 1319, 1436, 1895
XbaI	TCTAGA	1 cut(s)	1891
XceI	RCATGY	3 cut(s)	1218, 1531, 3279
XcmI	CCANNNNNNNNNTGG	1 cut(s)	652
XhoI	CTCGAG	1 cut(s)	1537
XmaI	CCCGGG	1 cut(s)	132
XmiI	GTMKAC	1 cut(s)	2925
XmnI	GAANNNNTTC	4 cut(s)	531, 1953, 2466, 3218
ZrmI	AGTACT	1 cut(s)	2175
Zsp2I	ATGCAT	2 cut(s)	2270, 3281

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.