MD10G1258800.v1.1

Thymidine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0014964
Physical Location & Seq
Forward (+)
35283795 .. 35287519
3725 bp
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UTR
Exon/CDS
Intron
MD10G1258800.v1.1.491

Sequence Viewer

Length: 846 bp
ATGTTAGCTATTTCGAGAATGAAGGCCTTCATCTCCTCCGCCCCTCACGTTTCCAAGACCACACCTTTCGCCCTTTTATCTTTAGCCTCCTCCTCCAAGTCCTTCTCTTTCAGCCCTCCAACCCTTTTCCAAAAACCCAATCTTCTCCTCCCGAAACCCAATATTTTTCTCCCCAAAAGATCCATTTTTTCACCTCAGAACGTCCGTTGTCTGCAAATGGAGGCGGAATCTCACCAGCGCTCCGGTGAGATTCACGTCATCGTCGGCCCCATGTTCGCCGGCAAGACCACCACGCTTCTTCGCAAAGTTCAGTCCGAGAGAGGCGACGGCAGAAATGTTGCTATAATCAAATCGATCAAGGATACAAGATATGGATTGGATTCAATTGTGACACATGACGGGGTCAAGTTGCCATGCTGGCCTTTGAAAGATTTATCATCGTTCAAACAAAAGTTTACCCCTGAGGCATATGATAAGCTAGATGTGATTGGTATCGACGAAGCTCAATTCTTTGAAGACCTGTATGATTTCTGCTCTGAAGCTGCTGATCATGATGGTAAAAAAGTGATAGTCGCCGGACTAGATGGTGACTATTTAAGGAGGAGCTTTGGTTCTGTGCTAGATATCATTCCCCTTGCTGACTCTGTAACCAAGTTAACTGCTCGATGCGAACTTTGTGGGAAACGTGCTTTCTTTACACTAAGAAAGACGGAGGAGTTGCAGACTGAACTCATTGGCGGGGCTGACGTCTACATGCCTGTCTGCCGGCAACACTACGTTGGTGGCCAAGTAGCCGTAGAAGCTGCGAGAGTTGTGCTGGAGTCTCAAGTTCAGTGCGCGTCATAG

Protein Analysis

282

Amino Acids

31.06

Weight (kDa)

8.61

Isoelectric Point (pI)

46.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TK PF00265 82 - 259 2.4e-54 Thymidine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014964)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23070
fragaria_vesca FvH4_3g00700
malus_domestica MD10G1258800.v1.1 MD10G1335800.v1.1
prunus_persica Prupe.4G007900_v2.0.a1
pyrus_communis pycom10g21530
rosa_chinensis RchiOBHm_Chr5g0000951
rosa_laevigata RLG00000030907
rosa_multiflora Rmu_sc0000255.1_g000020 Rmu_sc0019885.1_g000003
rosa_rugosa Rorug04G0388000
rosa_samantha Rh5AG007500 Rh5BG009600 Rh5CG008200 Rh5DG008200
rosa_wichuraiana Rw5G000780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 750
AccI GTMKAC 1 cut(s) 750
AccII CGCG 1 cut(s) 839
AciI CCGC 3 cut(s) 39, 224, 738
AclWI GGATC 1 cut(s) 174
AcoI YGGCCR 1 cut(s) 784
AcuI CTGAAG 1 cut(s) 558
AcyI GRCGYC 1 cut(s) 747
AfeI AGCGCT 1 cut(s) 239
AgsI TTSAA 4 cut(s) 384, 427, 445, 515
AjiI CACGTC 1 cut(s) 256
AloI GAACNNNNNNTCC 2 cut(s) 595, 627
AluBI AGCT 6 cut(s) 8, 478, 503, 542, 606, 803
AluI AGCT 6 cut(s) 8, 478, 503, 542, 606, 803
Alw26I GTCTC 1 cut(s) 828
AlwI GGATC 1 cut(s) 174
Aor51HI AGCGCT 1 cut(s) 239
AoxI GGCC 4 cut(s) 24, 265, 419, 784
ApeKI GCWGC 2 cut(s) 542, 803
Asp700I GAANNNNTTC 1 cut(s) 26
AspLEI GCGC 2 cut(s) 240, 839
AspS9I GGNCC 1 cut(s) 266
AsuHPI GGTGA 4 cut(s) 183, 224, 257, 599
AxyI CCTNAGG 1 cut(s) 462
BalI TGGCCA 1 cut(s) 786
BbsI GAAGAC 1 cut(s) 522
BbvI GCAGC 2 cut(s) 529, 790
BccI CCATC 2 cut(s) 548, 578
BceAI ACGGC 2 cut(s) 343, 779
BcgI CGANNNNNNTGC 2 cut(s) 796, 830
BciVI GTATCC 1 cut(s) 355
BclI TGATCA 1 cut(s) 547
BcoDI GTCTC 1 cut(s) 828
BfaI CTAG 3 cut(s) 479, 581, 620
BfoI RGCGCY 1 cut(s) 241
BfuI GTATCC 1 cut(s) 355
BglI GCCNNNNNGGC 1 cut(s) 418
BisI GCNGC 2 cut(s) 543, 804
BlsI GCNGC 2 cut(s) 544, 805
BmgBI CACGTC 1 cut(s) 256
BmgT120I GGNCC 1 cut(s) 266
BmiI GGNNCC 1 cut(s) 268
BmsI GCATC 1 cut(s) 656
BpiI GAAGAC 1 cut(s) 522
BpmI CTGGAG 1 cut(s) 839
BpuEI CTTGAG 1 cut(s) 810
Bsa29I ATCGAT 1 cut(s) 353
BsaBI GATNNNNATC 1 cut(s) 491
BsaHI GRCGYC 1 cut(s) 747
BsaWI WCCGGW 1 cut(s) 242
BsaXI ACNNNNNCTCC 4 cut(s) 224, 254, 595, 625
Bse118I RCCGGY 2 cut(s) 278, 765
Bse21I CCTNAGG 1 cut(s) 462
Bse8I GATNNNNATC 1 cut(s) 491
BseCI ATCGAT 1 cut(s) 353
BseJI GATNNNNATC 1 cut(s) 491
BseMII CTCAG 2 cut(s) 209, 453
BseRI GAGGAG 6 cut(s) 25, 79, 82, 137, 616, 728
BseXI GCAGC 2 cut(s) 529, 790
Bsh1236I CGCG 1 cut(s) 839
BshFI GGCC 4 cut(s) 26, 267, 421, 786
BshVI ATCGAT 1 cut(s) 353
BsiSI CCGG 4 cut(s) 243, 279, 576, 766
BsmAI GTCTC 1 cut(s) 828
BsnI GGCC 4 cut(s) 26, 267, 421, 786
Bsp143I GATC 3 cut(s) 179, 354, 547
BspACI CCGC 3 cut(s) 39, 224, 738
BspANI GGCC 4 cut(s) 26, 267, 421, 786
BspCNI CTCAG 2 cut(s) 208, 454
BspDI ATCGAT 1 cut(s) 353
BspFNI CGCG 1 cut(s) 839
BspHI TCATGA 1 cut(s) 550
BspLI GGNNCC 1 cut(s) 268
BspPI GGATC 1 cut(s) 174
BsrFI RCCGGY 2 cut(s) 278, 765
BssAI RCCGGY 2 cut(s) 278, 765
BssMI GATC 3 cut(s) 179, 354, 547
BssNI GRCGYC 1 cut(s) 747
BstACI GRCGYC 1 cut(s) 747
BstC8I GCNNGC 3 cut(s) 280, 419, 767
BstDEI CTNAG 3 cut(s) 195, 462, 701
BstFNI CGCG 1 cut(s) 839
BstH2I RGCGCY 1 cut(s) 241
BstHHI GCGC 2 cut(s) 240, 839
BstKTI GATC 3 cut(s) 182, 357, 550
BstMAI GTCTC 1 cut(s) 828
BstMBI GATC 3 cut(s) 179, 354, 547
BstMWI GCNNNNNNNGC 2 cut(s) 418, 800
BstNSI RCATGY 1 cut(s) 757
BstUI CGCG 1 cut(s) 839
BstV1I GCAGC 2 cut(s) 529, 790
BstV2I GAAGAC 1 cut(s) 522
BstX2I RGATCY 1 cut(s) 179
BstYI RGATCY 1 cut(s) 179
Bsu15I ATCGAT 1 cut(s) 353
Bsu36I CCTNAGG 1 cut(s) 462
BsuI GTATCC 1 cut(s) 355
BsuRI GGCC 4 cut(s) 26, 267, 421, 786
BsuTUI ATCGAT 1 cut(s) 353
BtrI CACGTC 1 cut(s) 256
BtsIMutI CAGTG 1 cut(s) 839
Cac8I GCNNGC 3 cut(s) 280, 419, 767
CciI TCATGA 1 cut(s) 550
CfoI GCGC 2 cut(s) 240, 839
Cfr10I RCCGGY 2 cut(s) 278, 765
Cfr13I GGNCC 1 cut(s) 266
ClaI ATCGAT 1 cut(s) 353
CseI GACGC 1 cut(s) 828
CviAII CATG 5 cut(s) 271, 395, 414, 551, 754
DdeI CTNAG 3 cut(s) 195, 462, 701
DpnI GATC 3 cut(s) 181, 356, 549
DpnII GATC 3 cut(s) 179, 354, 547
EaeI YGGCCR 1 cut(s) 784
EciI GGCGGA 2 cut(s) 28, 239
Eco147I AGGCCT 1 cut(s) 26
Eco32I GATATC 1 cut(s) 625
Eco47III AGCGCT 1 cut(s) 239
Eco57I CTGAAG 1 cut(s) 558
Eco81I CCTNAGG 1 cut(s) 462
EcoRV GATATC 1 cut(s) 625
FaeI CATG 5 cut(s) 274, 398, 417, 554, 757
FatI CATG 5 cut(s) 270, 394, 413, 550, 753
FauI CCCGC 1 cut(s) 731
FauNDI CATATG 1 cut(s) 469
FbaI TGATCA 1 cut(s) 547
FblI GTMKAC 1 cut(s) 750
Fnu4HI GCNGC 2 cut(s) 543, 804
Fsp4HI GCNGC 2 cut(s) 543, 804
FspBI CTAG 3 cut(s) 479, 581, 620
GlaI GCGC 2 cut(s) 239, 838
GluI GCNGC 2 cut(s) 543, 804
GsuI CTGGAG 1 cut(s) 839
HaeII RGCGCY 1 cut(s) 241
HaeIII GGCC 4 cut(s) 26, 267, 421, 786
HapII CCGG 4 cut(s) 243, 279, 576, 766
HgaI GACGC 1 cut(s) 828
HhaI GCGC 2 cut(s) 240, 839
Hin1I GRCGYC 1 cut(s) 747
Hin1II CATG 5 cut(s) 274, 398, 417, 554, 757
Hin6I GCGC 2 cut(s) 238, 837
HinP1I GCGC 2 cut(s) 238, 837
HincII GTYRAC 1 cut(s) 657
HindII GTYRAC 1 cut(s) 657
HinfI GANTC 5 cut(s) 227, 250, 380, 641, 821
HpaI GTTAAC 1 cut(s) 657
HpaII CCGG 4 cut(s) 243, 279, 576, 766
HphI GGTGA 4 cut(s) 183, 224, 257, 599
Hpy166II GTNNAC 3 cut(s) 456, 657, 751
Hpy188I TCNGA 3 cut(s) 198, 316, 538
Hpy188III TCNNGA 3 cut(s) 15, 151, 551
Hpy8I GTNNAC 3 cut(s) 456, 657, 751
Hpy99I CGWCG 3 cut(s) 266, 329, 500
HpyAV CCTTC 3 cut(s) 16, 37, 112
HpyCH4IV ACGT 6 cut(s) 48, 201, 255, 685, 747, 777
HpyCH4V TGCA 2 cut(s) 214, 721
HpyF10VI GCNNNNNNNGC 2 cut(s) 418, 800
HpyF3I CTNAG 3 cut(s) 195, 462, 701
HpySE526I ACGT 6 cut(s) 48, 201, 255, 685, 747, 777
Hsp92I GRCGYC 1 cut(s) 747
Hsp92II CATG 5 cut(s) 274, 398, 417, 554, 757
HspAI GCGC 2 cut(s) 238, 837
KroI GCCGGC 2 cut(s) 278, 765
KroNI GCCGGC 2 cut(s) 280, 767
Ksp22I TGATCA 1 cut(s) 547
KspAI GTTAAC 1 cut(s) 657
Kzo9I GATC 3 cut(s) 179, 354, 547
LmnI GCTCC 2 cut(s) 245, 603
Lsp1109I GCAGC 2 cut(s) 529, 790
LweI GCATC 1 cut(s) 656
MaeI CTAG 3 cut(s) 479, 581, 620
MaeII ACGT 6 cut(s) 48, 201, 255, 685, 747, 777
MaeIII GTNAC 3 cut(s) 388, 587, 646
MalI GATC 3 cut(s) 181, 356, 549
MboI GATC 3 cut(s) 179, 354, 547
MboII GAAGA 3 cut(s) 134, 290, 527
MfeI CAATTG 1 cut(s) 384
MflI RGATCY 1 cut(s) 179
MlsI TGGCCA 1 cut(s) 786
MluCI AATT 2 cut(s) 384, 506
MluNI TGGCCA 1 cut(s) 786
MlyI GAGTC 2 cut(s) 635, 830
MmeI TCCRAC 1 cut(s) 143
Mox20I TGGCCA 1 cut(s) 786
MroNI GCCGGC 2 cut(s) 278, 765
MroXI GAANNNNTTC 1 cut(s) 26
MscI TGGCCA 1 cut(s) 786
MseI TTAA 2 cut(s) 596, 656
Msp20I TGGCCA 1 cut(s) 786
MspI CCGG 4 cut(s) 243, 279, 576, 766
MunI CAATTG 1 cut(s) 384
MvnI CGCG 1 cut(s) 839
MwoI GCNNNNNNNGC 2 cut(s) 418, 800
NaeI GCCGGC 2 cut(s) 280, 767
NdeI CATATG 1 cut(s) 469
NdeII GATC 3 cut(s) 179, 354, 547
NgoMIV GCCGGC 2 cut(s) 278, 765
NlaIII CATG 5 cut(s) 274, 398, 417, 554, 757
NlaIV GGNNCC 1 cut(s) 268
NmuCI GTSAC 2 cut(s) 388, 587
NspI RCATGY 1 cut(s) 757
PagI TCATGA 1 cut(s) 550
PceI AGGCCT 1 cut(s) 26
PdiI GCCGGC 2 cut(s) 280, 767
PdmI GAANNNNTTC 1 cut(s) 26
PfeI GAWTC 3 cut(s) 227, 250, 380
PflFI GACNNNGTC 1 cut(s) 401
PkrI GCNGC 2 cut(s) 544, 805
PleI GAGTC 2 cut(s) 635, 829
PpsI GAGTC 2 cut(s) 635, 829
PspN4I GGNNCC 1 cut(s) 268
PspPI GGNCC 1 cut(s) 266
PsuI RGATCY 1 cut(s) 179
PsyI GACNNNGTC 1 cut(s) 401
SaqAI TTAA 2 cut(s) 596, 656
SatI GCNGC 2 cut(s) 543, 804
Sau3AI GATC 3 cut(s) 179, 354, 547
Sau96I GGNCC 1 cut(s) 266
SchI GAGTC 2 cut(s) 635, 830
SfaNI GCATC 1 cut(s) 656
SmlI CTYRAG 1 cut(s) 825
SmoI CTYRAG 1 cut(s) 825
Sse9I AATT 2 cut(s) 384, 506
SseBI AGGCCT 1 cut(s) 26
SsiI CCGC 3 cut(s) 39, 224, 738
SspI AATATT 1 cut(s) 163
SspMI CTAG 3 cut(s) 479, 581, 620
StuI AGGCCT 1 cut(s) 26
TaiI ACGT 6 cut(s) 51, 204, 258, 688, 750, 780
TaqI TCGA 4 cut(s) 14, 353, 495, 664
TasI AATT 2 cut(s) 384, 506
TfiI GAWTC 3 cut(s) 227, 250, 380
Tru1I TTAA 2 cut(s) 596, 656
Tru9I TTAA 2 cut(s) 596, 656
TscAI CASTG 1 cut(s) 839
TseFI GTSAC 2 cut(s) 388, 587
TseI GCWGC 2 cut(s) 542, 803
Tsp45I GTSAC 2 cut(s) 388, 587
TspDTI ATGAA 2 cut(s) 19, 35
TspGWI ACGGA 2 cut(s) 194, 725
TspRI CASTG 1 cut(s) 839
Tth111I GACNNNGTC 1 cut(s) 401
XceI RCATGY 1 cut(s) 757
XmiI GTMKAC 1 cut(s) 750
XmnI GAANNNNTTC 1 cut(s) 26
XspI CTAG 3 cut(s) 479, 581, 620
ZraI GACGTC 1 cut(s) 748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.