RLG00000030907

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0014964
Physical Location & Seq
Reverse (-)
549462 .. 550694
1233 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030907

Sequence Viewer

Length: 597 bp
ATGCAGAATTCTTGGTGGGATTCTAATGATGTTGCTATACAGCACATAGAGGTAGAGATATTTTTCCAATCTAAAGTTAATGTTGCAGTGATAAAATCAAACAAGGATACAAGATATGGATTGGATTCGATTGTAACACATGATGGGGTTAAATTGCCTTGCTGGGCATTACCAAATCTATCAGTGTTCAAACAAAAGTTTGGTCTTGATGCATATGAGCAGCTAGATGTAATCGGTATTGATGAAGCTCAGTTCTTTGAAGATCTTTATGATTTCTGCCGTGAAGCTGCTGATCATGATGGCAAAAAAGTAATAGTTGCTGGACTAGATGGGGACTATTTGAGGAGGAGCTTTGGATCTGTGCTTGATATAATTCCCCTTGCTGATTCTGTAACCAAGTTAACTTCTCGATGCGAACTTTGTGGGAAACCGGCTTTCTTTACGTTACGAAAGACGGAGGAGATGCAGACGGAAGTGATTGGCGGGGCTGATGTGTACATGCCTGTGTGTCGACAGCACTATGTTAGTGGACAAGTAGCCATTCAAGCTGCACGAGTTGTCCTGGAATCTCAAGTTGAGTGTAGGTCTTGTGCGTAG

Protein Analysis

199

Amino Acids

22.21

Weight (kDa)

4.9

Isoelectric Point (pI)

44.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TK PF00265 19 - 174 1.4e-43 Thymidine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014964)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23070
fragaria_vesca FvH4_3g00700
malus_domestica MD10G1258800.v1.1 MD10G1335800.v1.1
prunus_persica Prupe.4G007900_v2.0.a1
pyrus_communis pycom10g21530
rosa_chinensis RchiOBHm_Chr5g0000951
rosa_laevigata RLG00000030907
rosa_multiflora Rmu_sc0000255.1_g000020 Rmu_sc0019885.1_g000003
rosa_rugosa Rorug04G0388000
rosa_samantha Rh5AG007500 Rh5BG009600 Rh5CG008200 Rh5DG008200
rosa_wichuraiana Rw5G000780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 511
AciI CCGC 1 cut(s) 483
AclWI GGATC 1 cut(s) 364
AcsI RAATTY 1 cut(s) 7
AfaI GTAC 1 cut(s) 497
AgsI TTSAA 3 cut(s) 190, 260, 545
AjnI CCWGG 1 cut(s) 561
AluBI AGCT 5 cut(s) 223, 248, 287, 351, 548
AluI AGCT 5 cut(s) 223, 248, 287, 351, 548
AlwI GGATC 1 cut(s) 364
ApeKI GCWGC 3 cut(s) 220, 287, 548
ApoI RAATTY 1 cut(s) 7
ArsI GACNNNNNNTTYG 2 cut(s) 187, 219
BauI CACGAG 1 cut(s) 552
BbvI GCAGC 3 cut(s) 232, 274, 535
BccI CCATC 3 cut(s) 137, 293, 323
BceAI ACGGC 1 cut(s) 264
BciT130I CCWGG 1 cut(s) 563
BciVI GTATCC 1 cut(s) 100
BclI TGATCA 1 cut(s) 292
BfaI CTAG 2 cut(s) 224, 326
BfuI GTATCC 1 cut(s) 100
BglII AGATCT 1 cut(s) 262
BisI GCNGC 3 cut(s) 221, 288, 549
BlsI GCNGC 3 cut(s) 222, 289, 550
Bme1390I CCNGG 1 cut(s) 563
BmrFI CCNGG 1 cut(s) 563
BmsI GCATC 3 cut(s) 199, 401, 453
BpuEI CTTGAG 1 cut(s) 555
Bse118I RCCGGY 1 cut(s) 430
BseBI CCWGG 1 cut(s) 563
BseMII CTCAG 1 cut(s) 263
BseRI GAGGAG 3 cut(s) 358, 361, 473
BseXI GCAGC 3 cut(s) 232, 274, 535
BseYI CCCAGC 1 cut(s) 162
BsgI GTGCAG 1 cut(s) 534
BsiSI CCGG 1 cut(s) 431
BslFI GGGAC 1 cut(s) 347
BsmFI GGGAC 1 cut(s) 347
Bsp1407I TGTACA 1 cut(s) 495
Bsp143I GATC 3 cut(s) 262, 292, 356
BspACI CCGC 1 cut(s) 483
BspCNI CTCAG 1 cut(s) 262
BspHI TCATGA 1 cut(s) 295
BspPI GGATC 1 cut(s) 364
BsrFI RCCGGY 1 cut(s) 430
BsrGI TGTACA 1 cut(s) 495
BssAI RCCGGY 1 cut(s) 430
BssMI GATC 3 cut(s) 262, 292, 356
BssSI CACGAG 1 cut(s) 552
Bst2BI CACGAG 1 cut(s) 552
Bst2UI CCWGG 1 cut(s) 563
BstAUI TGTACA 1 cut(s) 495
BstDEI CTNAG 1 cut(s) 249
BstKTI GATC 3 cut(s) 265, 295, 359
BstMBI GATC 3 cut(s) 262, 292, 356
BstMWI GCNNNNNNNGC 1 cut(s) 545
BstNI CCWGG 1 cut(s) 563
BstNSI RCATGY 1 cut(s) 502
BstSCI CCNGG 1 cut(s) 561
BstV1I GCAGC 3 cut(s) 232, 274, 535
BstX2I RGATCY 2 cut(s) 262, 356
BstYI RGATCY 2 cut(s) 262, 356
BsuI GTATCC 1 cut(s) 100
BtsI GCAGTG 1 cut(s) 93
BtsIMutI CAGTG 2 cut(s) 93, 189
CciI TCATGA 1 cut(s) 295
Cfr10I RCCGGY 1 cut(s) 430
Csp6I GTAC 1 cut(s) 496
CviAII CATG 3 cut(s) 140, 296, 499
CviJI RGCY 8 cut(s) 223, 248, 287, 351, 434, 488, 539, 548
CviKI_1 RGCY 8 cut(s) 223, 248, 287, 351, 434, 488, 539, 548
CviQI GTAC 1 cut(s) 496
DdeI CTNAG 1 cut(s) 249
DpnI GATC 3 cut(s) 264, 294, 358
DpnII GATC 3 cut(s) 262, 292, 356
EcoRI GAATTC 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 561
EcoT22I ATGCAT 1 cut(s) 214
FaeI CATG 3 cut(s) 143, 299, 502
FaqI GGGAC 1 cut(s) 347
FatI CATG 3 cut(s) 139, 295, 498
FauI CCCGC 1 cut(s) 476
FauNDI CATATG 1 cut(s) 214
FbaI TGATCA 1 cut(s) 292
FblI GTMKAC 1 cut(s) 511
Fnu4HI GCNGC 3 cut(s) 221, 288, 549
Fsp4HI GCNGC 3 cut(s) 221, 288, 549
FspBI CTAG 2 cut(s) 224, 326
GluI GCNGC 3 cut(s) 221, 288, 549
GsaI CCCAGC 1 cut(s) 166
HapII CCGG 1 cut(s) 431
Hin1II CATG 3 cut(s) 143, 299, 502
HincII GTYRAC 2 cut(s) 402, 512
HindII GTYRAC 2 cut(s) 402, 512
HinfI GANTC 4 cut(s) 20, 125, 386, 566
HpaI GTTAAC 1 cut(s) 402
HpaII CCGG 1 cut(s) 431
Hpy166II GTNNAC 4 cut(s) 402, 496, 512, 530
Hpy188III TCNNGA 3 cut(s) 206, 296, 408
Hpy8I GTNNAC 4 cut(s) 402, 496, 512, 530
HpyCH4IV ACGT 1 cut(s) 443
HpyCH4V TGCA 5 cut(s) 4, 86, 212, 466, 551
HpyF10VI GCNNNNNNNGC 1 cut(s) 545
HpyF3I CTNAG 1 cut(s) 249
HpySE526I ACGT 1 cut(s) 443
Hsp92II CATG 3 cut(s) 143, 299, 502
Ksp22I TGATCA 1 cut(s) 292
KspAI GTTAAC 1 cut(s) 402
Kzo9I GATC 3 cut(s) 262, 292, 356
LmnI GCTCC 1 cut(s) 348
LpnPI CCDG 6 cut(s) 148, 306, 444, 516, 548, 575
Lsp1109I GCAGC 3 cut(s) 232, 274, 535
LweI GCATC 3 cut(s) 199, 401, 453
MaeI CTAG 2 cut(s) 224, 326
MaeII ACGT 1 cut(s) 443
MaeIII GTNAC 3 cut(s) 133, 391, 444
MalI GATC 3 cut(s) 264, 294, 358
MboI GATC 3 cut(s) 262, 292, 356
MboII GAAGA 1 cut(s) 272
MflI RGATCY 2 cut(s) 262, 356
MluCI AATT 3 cut(s) 7, 152, 372
MnlI CCTC 4 cut(s) 43, 336, 339, 451
Mph1103I ATGCAT 1 cut(s) 214
MseI TTAA 3 cut(s) 78, 150, 401
MslI CAYNNNNRTG 1 cut(s) 503
MspI CCGG 1 cut(s) 431
MspR9I CCNGG 1 cut(s) 563
MvaI CCWGG 1 cut(s) 563
MwoI GCNNNNNNNGC 1 cut(s) 545
NdeI CATATG 1 cut(s) 214
NdeII GATC 3 cut(s) 262, 292, 356
NlaIII CATG 3 cut(s) 143, 299, 502
NsiI ATGCAT 1 cut(s) 214
NspI RCATGY 1 cut(s) 502
PagI TCATGA 1 cut(s) 295
PfeI GAWTC 4 cut(s) 20, 125, 386, 566
PfoI TCCNGGA 1 cut(s) 561
PkrI GCNGC 3 cut(s) 222, 289, 550
Psp6I CCWGG 1 cut(s) 561
PspFI CCCAGC 1 cut(s) 162
PspGI CCWGG 1 cut(s) 561
PsuI RGATCY 2 cut(s) 262, 356
RsaI GTAC 1 cut(s) 497
RsaNI GTAC 1 cut(s) 496
RseI CAYNNNNRTG 1 cut(s) 503
SalI GTCGAC 1 cut(s) 510
SaqAI TTAA 3 cut(s) 78, 150, 401
SatI GCNGC 3 cut(s) 221, 288, 549
Sau3AI GATC 3 cut(s) 262, 292, 356
ScrFI CCNGG 1 cut(s) 563
SetI ASST 8 cut(s) 54, 225, 250, 289, 353, 446, 550, 587
SfaNI GCATC 3 cut(s) 199, 401, 453
SmiMI CAYNNNNRTG 1 cut(s) 503
SmlI CTYRAG 1 cut(s) 570
SmoI CTYRAG 1 cut(s) 570
Sse9I AATT 3 cut(s) 7, 152, 372
SsiI CCGC 1 cut(s) 483
SspMI CTAG 2 cut(s) 224, 326
StyD4I CCNGG 1 cut(s) 561
TaiI ACGT 1 cut(s) 446
TaqI TCGA 3 cut(s) 128, 409, 511
TasI AATT 3 cut(s) 7, 152, 372
TatI WGTACW 1 cut(s) 495
TfiI GAWTC 4 cut(s) 20, 125, 386, 566
Tru1I TTAA 3 cut(s) 78, 150, 401
Tru9I TTAA 3 cut(s) 78, 150, 401
TscAI CASTG 2 cut(s) 93, 189
TseI GCWGC 3 cut(s) 220, 287, 548
TspDTI ATGAA 1 cut(s) 258
TspGWI ACGGA 2 cut(s) 470, 485
TspRI CASTG 2 cut(s) 93, 189
XapI RAATTY 1 cut(s) 7
XceI RCATGY 1 cut(s) 502
XmiI GTMKAC 1 cut(s) 511
XspI CTAG 2 cut(s) 224, 326
Zsp2I ATGCAT 1 cut(s) 214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.