Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0014964

Physical Location & Seq

Genomic Coordinates Forward (+)

41257173 .. 41260911

Gene Structure 3739 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1335800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 894 bp

ATGTTAGGTATTTCAAGATCAAGAATGAAGGCCCTCCTCATCTCCTCCGTCCCTCACATCTCAAAGATCACACCTTTATCTTTAGCCTCCTCCTCCTCCTCCTCCTCCAAGTCCTTTTCCTTCAGCCCTCCTACTCTTTCCCAGAACCCCAATATTCTCCTCCCAAAACCCAATATTTTTCTCCCCAAAAGCACCATTTATTCAGCTCAGAACATCCGATGTGTGCAAATGGAGGCAGAATCTCACCAACGCTCCGGTGAGACTCACGTCATCGTCGGCCCCATGTTCGCCGGAAAGACCACCACGCTTCTTCGTGAAGTTCAGTCCGAGAGAGGCGATGGCAGAAATGTTGCTATAATCAAATCGATCAAGGATACAAGATATGGATTGGATTCAATTGTGACGCATGACGGGGTCAAGTTCCCATGCTGGCCTTTAAAAGATCTATCCTCGTTCAAACAAAAGTTTAGCCCTGAGGCATACGATCAGCTAGACGTGATTGGTATCGATGAAGCTCAGTTCTTTGAAGACCTCTATGATTTCTGCTCTGAAGCTGCTGATCGTGATGGTAAAAAAGTAATAGTCGCCGGACTAGATGGTGACTATTTAAGGAGGAAATTTGGTTCTGTGTTAGATATAGTTCCCCTCGCTGATTCTGTAACCAAGTTAACTGCTCGGTGCGAACTTTGTGGGAAACGTGCTTTCTTTACACTAAGAAAGACGGAGGAGATGCAAACTGAACTGATTGGCGGGGCTGACGTTTACATGCCTGTCTGCCGGCAACACTACGTTGCTGGACAGGTAGCCATAGAAGCCGCGAGAGTTGTAAGGAGTCTCAAGTTCAGTGCGGGTCATATGCATAGTAACCTTTTCCAGTCAGTAGGAAGTAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004797 GO:0005488 GO:0005515 GO:0006139 GO:0006213 GO:0006259 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009058 GO:0009116 GO:0009117 GO:0009120 GO:0009123 GO:0009124 GO:0009157 GO:0009162 GO:0009165 GO:0009266 GO:0009409 GO:0009628 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0018130 GO:0019136 GO:0019205 GO:0019206 GO:0019438 GO:0019637 GO:0034641 GO:0034654 GO:0042802 GO:0043170 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044281 GO:0046104 GO:0046125 GO:0046483 GO:0050896 GO:0055086 GO:0071704 GO:0072527 GO:0090304 GO:0090407 GO:1901135 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901576 GO:1901657

KEGG Pathways

Metabolic & Signaling

ko00240 ko00983 ko01100 map00240 map00983 map01100

Pfam Domains

Protein Families

Protein Analysis

298

Amino Acids

32.79

Weight (kDa)

8.96

Isoelectric Point (pI)

51.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TK	PF00265	87 - 263	4.9e-53	Thymidine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0014964)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23070
fragaria_vesca	FvH4_3g00700
malus_domestica	MD10G1258800.v1.1 MD10G1335800.v1.1
prunus_persica	Prupe.4G007900_v2.0.a1
pyrus_communis	pycom10g21530
rosa_chinensis	RchiOBHm_Chr5g0000951
rosa_laevigata	RLG00000030907
rosa_multiflora	Rmu_sc0000255.1_g000020 Rmu_sc0019885.1_g000003
rosa_rugosa	Rorug04G0388000
rosa_samantha	Rh5AG007500 Rh5BG009600 Rh5CG008200 Rh5DG008200
rosa_wichuraiana	Rw5G000780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	818
AciI	CCGC	3 cut(s)	750, 816, 848
AcsI	RAATTY	1 cut(s)	617
AcuI	CTGAAG	2 cut(s)	106, 570
AgsI	TTSAA	4 cut(s)	15, 396, 457, 527
AjiI	CACGTC	2 cut(s)	268, 496
AloI	GAACNNNNNNTCC	2 cut(s)	607, 639
AluBI	AGCT	4 cut(s)	206, 490, 515, 554
AluI	AGCT	4 cut(s)	206, 490, 515, 554
Alw26I	GTCTC	2 cut(s)	254, 839
AoxI	GGCC	3 cut(s)	30, 277, 431
ApeKI	GCWGC	1 cut(s)	554
ApoI	RAATTY	1 cut(s)	617
AspS9I	GGNCC	2 cut(s)	31, 278
AsuHPI	GGTGA	3 cut(s)	236, 269, 611
AxyI	CCTNAGG	1 cut(s)	474
BbsI	GAAGAC	1 cut(s)	534
BbvI	GCAGC	1 cut(s)	541
BccI	CCATC	3 cut(s)	332, 560, 590
BciVI	GTATCC	1 cut(s)	367
BcoDI	GTCTC	2 cut(s)	254, 839
BfaI	CTAG	2 cut(s)	491, 593
BfuI	GTATCC	1 cut(s)	367
BglII	AGATCT	1 cut(s)	442
BisI	GCNGC	2 cut(s)	555, 816
BlsI	GCNGC	2 cut(s)	556, 817
BmgBI	CACGTC	2 cut(s)	268, 496
BmgT120I	GGNCC	2 cut(s)	31, 278
BmiI	GGNNCC	1 cut(s)	280
BmsI	GCATC	1 cut(s)	720
BoxI	GACNNNNGTC	1 cut(s)	266
BpiI	GAAGAC	1 cut(s)	534
BpuEI	CTTGAG	1 cut(s)	821
Bsa29I	ATCGAT	2 cut(s)	365, 507
BsaBI	GATNNNNATC	1 cut(s)	503
BsaWI	WCCGGW	1 cut(s)	254
BsaXI	ACNNNNNCTCC	2 cut(s)	236, 266
Bse118I	RCCGGY	1 cut(s)	777
Bse1I	ACTGG	1 cut(s)	874
Bse21I	CCTNAGG	1 cut(s)	474
Bse8I	GATNNNNATC	1 cut(s)	503
BseCI	ATCGAT	2 cut(s)	365, 507
BseGI	GGATG	1 cut(s)	213
BseJI	GATNNNNATC	1 cut(s)	503
BseMII	CTCAG	3 cut(s)	221, 465, 530
BseNI	ACTGG	1 cut(s)	874
BseXI	GCAGC	1 cut(s)	541
Bsh1236I	CGCG	1 cut(s)	818
BshFI	GGCC	3 cut(s)	32, 279, 433
BshVI	ATCGAT	2 cut(s)	365, 507
BsiSI	CCGG	4 cut(s)	255, 291, 588, 778
BslFI	GGGAC	1 cut(s)	35
BsmAI	GTCTC	2 cut(s)	254, 839
BsmFI	GGGAC	1 cut(s)	35
BsnI	GGCC	3 cut(s)	32, 279, 433
Bsp143I	GATC	6 cut(s)	17, 66, 366, 442, 484, 559
BspACI	CCGC	3 cut(s)	750, 816, 848
BspANI	GGCC	3 cut(s)	32, 279, 433
BspCNI	CTCAG	3 cut(s)	220, 466, 529
BspDI	ATCGAT	2 cut(s)	365, 507
BspFNI	CGCG	1 cut(s)	818
BspLI	GGNNCC	1 cut(s)	280
BsrFI	RCCGGY	1 cut(s)	777
BsrI	ACTGG	1 cut(s)	874
BssAI	RCCGGY	1 cut(s)	777
BssMI	GATC	6 cut(s)	17, 66, 366, 442, 484, 559
BstC8I	GCNNGC	2 cut(s)	431, 779
BstDEI	CTNAG	4 cut(s)	207, 474, 516, 713
BstF5I	GGATG	1 cut(s)	213
BstFNI	CGCG	1 cut(s)	818
BstKTI	GATC	6 cut(s)	20, 69, 369, 445, 487, 562
BstMAI	GTCTC	2 cut(s)	254, 839
BstMBI	GATC	6 cut(s)	17, 66, 366, 442, 484, 559
BstMWI	GCNNNNNNNGC	1 cut(s)	812
BstNSI	RCATGY	1 cut(s)	769
BstPAI	GACNNNNGTC	1 cut(s)	266
BstUI	CGCG	1 cut(s)	818
BstV1I	GCAGC	1 cut(s)	541
BstV2I	GAAGAC	1 cut(s)	534
BstX2I	RGATCY	1 cut(s)	442
BstYI	RGATCY	1 cut(s)	442
Bsu15I	ATCGAT	2 cut(s)	365, 507
Bsu36I	CCTNAGG	1 cut(s)	474
BsuI	GTATCC	1 cut(s)	367
BsuRI	GGCC	3 cut(s)	32, 279, 433
BsuTUI	ATCGAT	2 cut(s)	365, 507
BtgZI	GCGATG	1 cut(s)	351
BtrI	CACGTC	2 cut(s)	268, 496
BtsCI	GGATG	1 cut(s)	213
BtsIMutI	CAGTG	1 cut(s)	850
Cac8I	GCNNGC	2 cut(s)	431, 779
Cfr10I	RCCGGY	1 cut(s)	777
Cfr13I	GGNCC	2 cut(s)	31, 278
ClaI	ATCGAT	2 cut(s)	365, 507
CseI	GACGC	1 cut(s)	412
CviAII	CATG	4 cut(s)	283, 407, 426, 766
DdeI	CTNAG	4 cut(s)	207, 474, 516, 713
DpnI	GATC	6 cut(s)	19, 68, 368, 444, 486, 561
DpnII	GATC	6 cut(s)	17, 66, 366, 442, 484, 559
DraI	TTTAAA	1 cut(s)	438
Eco57I	CTGAAG	2 cut(s)	106, 570
Eco81I	CCTNAGG	1 cut(s)	474
EcoO109I	RGGNCCY	1 cut(s)	31
EcoT22I	ATGCAT	1 cut(s)	861
FaeI	CATG	4 cut(s)	286, 410, 429, 769
FaqI	GGGAC	1 cut(s)	35
FatI	CATG	4 cut(s)	282, 406, 425, 765
FauI	CCCGC	2 cut(s)	743, 841
FauNDI	CATATG	1 cut(s)	855
Fnu4HI	GCNGC	2 cut(s)	555, 816
FokI	GGATG	1 cut(s)	200
Fsp4HI	GCNGC	2 cut(s)	555, 816
FspBI	CTAG	2 cut(s)	491, 593
GluI	GCNGC	2 cut(s)	555, 816
HaeIII	GGCC	3 cut(s)	32, 279, 433
HapII	CCGG	4 cut(s)	255, 291, 588, 778
HgaI	GACGC	1 cut(s)	412
Hin1II	CATG	4 cut(s)	286, 410, 429, 769
HincII	GTYRAC	1 cut(s)	669
HindII	GTYRAC	1 cut(s)	669
HinfI	GANTC	5 cut(s)	239, 262, 392, 653, 832
HpaI	GTTAAC	1 cut(s)	669
HpaII	CCGG	4 cut(s)	255, 291, 588, 778
HphI	GGTGA	3 cut(s)	236, 269, 611
Hpy166II	GTNNAC	2 cut(s)	669, 763
Hpy188I	TCNGA	4 cut(s)	210, 218, 328, 550
Hpy188III	TCNNGA	4 cut(s)	15, 21, 314, 563
Hpy8I	GTNNAC	2 cut(s)	669, 763
Hpy99I	CGWCG	1 cut(s)	278
HpyAV	CCTTC	2 cut(s)	22, 130
HpyCH4IV	ACGT	5 cut(s)	267, 495, 697, 759, 789
HpyCH4V	TGCA	3 cut(s)	226, 733, 859
HpyF10VI	GCNNNNNNNGC	1 cut(s)	812
HpyF3I	CTNAG	4 cut(s)	207, 474, 516, 713
HpySE526I	ACGT	5 cut(s)	267, 495, 697, 759, 789
Hsp92II	CATG	4 cut(s)	286, 410, 429, 769
KroI	GCCGGC	1 cut(s)	777
KroNI	GCCGGC	1 cut(s)	779
KspAI	GTTAAC	1 cut(s)	669
Kzo9I	GATC	6 cut(s)	17, 66, 366, 442, 484, 559
LmnI	GCTCC	1 cut(s)	257
Lsp1109I	GCAGC	1 cut(s)	541
LweI	GCATC	1 cut(s)	720
MaeI	CTAG	2 cut(s)	491, 593
MaeII	ACGT	5 cut(s)	267, 495, 697, 759, 789
MaeIII	GTNAC	4 cut(s)	400, 599, 658, 863
MalI	GATC	6 cut(s)	19, 68, 368, 444, 486, 561
MboI	GATC	6 cut(s)	17, 66, 366, 442, 484, 559
MboII	GAAGA	2 cut(s)	302, 539
MfeI	CAATTG	1 cut(s)	396
MflI	RGATCY	1 cut(s)	442
MluCI	AATT	2 cut(s)	396, 617
MlyI	GAGTC	2 cut(s)	256, 841
Mph1103I	ATGCAT	1 cut(s)	861
MroNI	GCCGGC	1 cut(s)	777
MseI	TTAA	3 cut(s)	437, 608, 668
MspI	CCGG	4 cut(s)	255, 291, 588, 778
MunI	CAATTG	1 cut(s)	396
MvnI	CGCG	1 cut(s)	818
MwoI	GCNNNNNNNGC	1 cut(s)	812
NaeI	GCCGGC	1 cut(s)	779
NdeI	CATATG	1 cut(s)	855
NdeII	GATC	6 cut(s)	17, 66, 366, 442, 484, 559
NgoMIV	GCCGGC	1 cut(s)	777
NlaIII	CATG	4 cut(s)	286, 410, 429, 769
NlaIV	GGNNCC	1 cut(s)	280
NmuCI	GTSAC	2 cut(s)	400, 599
NsiI	ATGCAT	1 cut(s)	861
NspI	RCATGY	1 cut(s)	769
PdiI	GCCGGC	1 cut(s)	779
PfeI	GAWTC	3 cut(s)	239, 392, 653
PflFI	GACNNNGTC	1 cut(s)	413
PkrI	GCNGC	2 cut(s)	556, 817
PleI	GAGTC	2 cut(s)	256, 840
PpsI	GAGTC	2 cut(s)	256, 840
PshAI	GACNNNNGTC	1 cut(s)	266
PspN4I	GGNNCC	1 cut(s)	280
PspPI	GGNCC	2 cut(s)	31, 278
PsuI	RGATCY	1 cut(s)	442
PsyI	GACNNNGTC	1 cut(s)	413
SaqAI	TTAA	3 cut(s)	437, 608, 668
SatI	GCNGC	2 cut(s)	555, 816
Sau3AI	GATC	6 cut(s)	17, 66, 366, 442, 484, 559
Sau96I	GGNCC	2 cut(s)	31, 278
SchI	GAGTC	2 cut(s)	256, 841
SfaNI	GCATC	1 cut(s)	720
SmlI	CTYRAG	1 cut(s)	836
SmoI	CTYRAG	1 cut(s)	836
Sse9I	AATT	2 cut(s)	396, 617
SsiI	CCGC	3 cut(s)	750, 816, 848
SspI	AATATT	2 cut(s)	154, 175
SspMI	CTAG	2 cut(s)	491, 593
TaiI	ACGT	5 cut(s)	270, 498, 700, 762, 792
TaqI	TCGA	2 cut(s)	365, 507
TasI	AATT	2 cut(s)	396, 617
TauI	GCSGC	1 cut(s)	818
TfiI	GAWTC	3 cut(s)	239, 392, 653
Tru1I	TTAA	3 cut(s)	437, 608, 668
Tru9I	TTAA	3 cut(s)	437, 608, 668
TscAI	CASTG	1 cut(s)	850
TseFI	GTSAC	2 cut(s)	400, 599
TseI	GCWGC	1 cut(s)	554
Tsp45I	GTSAC	2 cut(s)	400, 599
TspDTI	ATGAA	2 cut(s)	41, 525
TspGWI	ACGGA	2 cut(s)	37, 737
TspRI	CASTG	1 cut(s)	850
Tth111I	GACNNNGTC	1 cut(s)	413
XapI	RAATTY	1 cut(s)	617
XceI	RCATGY	1 cut(s)	769
XspI	CTAG	2 cut(s)	491, 593
Zsp2I	ATGCAT	1 cut(s)	861

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.