MD10G1269800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008634
Physical Location & Seq
Forward (+)
36223601 .. 36224434
834 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1269800.v1.1.491

Sequence Viewer

Length: 834 bp
ATGATGAGTCGGAGAAGACAACAAGGTCAAGGTACATACAGTCTTTATTCTCAAAAGCAGCGTCACCATCCCTGGACTAACGATCGCAGTCGGCGTCATGACGAGTCATTTACAAGTGTGCCTTACTGGGAAAAGAAATTCTGCACGTCCGTCGGCTCGGTTCCGTGGGGAAAACTCGTGGAAACGAAAAAGTACATGTCTCTGTACAAGAACATCGTTGAGTGGAAGGACTCGGCTGGCGAAGAGGCATTCAACAAGGCGAAAAGCCGGTTTTGGGCACAGATGAACGGCCTAATTCCCAGTTCTGACCTATCTTTGCCGGATCCCGACGTGTATATTGATGATATAGATTGGCAGTCGTCAACCGAAAAAATTGATCCTCAACTGATTTTGGACTTAGAAAAGAGCAAGGAACCTAAACCGGACAATGATCGTGGTGATGCTGAAACCCTAGGTCATGATCGTGAGTTTGTTATTATCGGCCTTCATCCTGTCAATATAAATCAGACGACATTGCCGTGCAGCGGATGGGGTGAGGAGTTTGAAGAAGAGGATTTGAAAAATAAAGAAAATAATAATATTCCTGAAAATCCATGGGAACCTGCTTGCGTTCGTCAGAGTGAAGCTGCAGCCGCGGGACGAGGATGGAACAACACAACCAGCTGGGAACAGTGGGAGAATAATAACTATAATTACAACTCAAGATATAAGAAGTCAAGATTTCATGGTGGTTATAATGATCACAAAGTTGGAGGGTCGTGGAGGAACAATGGAGGGAGAAGGAACAGATATTCAGTTCATGTTGGTAACCAAAACCTGGCTTCTTGCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

278

Amino Acids

32.19

Weight (kDa)

6.47

Isoelectric Point (pI)

54.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 735
AasI GACNNNNNNGTC 1 cut(s) 24
Acc36I ACCTGC 1 cut(s) 610
AccB7I CCANNNNNTGG 1 cut(s) 817
AccII CGCG 1 cut(s) 635
AciI CCGC 3 cut(s) 525, 633, 635
AclWI GGATC 3 cut(s) 317, 330, 371
AcsI RAATTY 1 cut(s) 137
AcyI GRCGYC 1 cut(s) 94
AfaI GTAC 3 cut(s) 34, 194, 206
AfiI CCNNNNNNNGG 3 cut(s) 274, 524, 817
AflIII ACRYGT 2 cut(s) 195, 330
AgsI TTSAA 3 cut(s) 253, 545, 559
AjiI CACGTC 2 cut(s) 147, 331
AjnI CCWGG 2 cut(s) 71, 816
AluBI AGCT 2 cut(s) 626, 663
AluI AGCT 2 cut(s) 626, 663
Alw26I GTCTC 1 cut(s) 204
AlwI GGATC 3 cut(s) 317, 330, 371
AoxI GGCC 2 cut(s) 289, 481
ApeKI GCWGC 4 cut(s) 58, 522, 626, 629
ApoI RAATTY 1 cut(s) 137
ArsI GACNNNNNNTTYG 2 cut(s) 46, 78
AspA2I CCTAGG 1 cut(s) 451
AsuHPI GGTGA 3 cut(s) 56, 449, 545
AvrII CCTAGG 1 cut(s) 451
BaeGI GKGCMC 1 cut(s) 280
BamHI GGATCC 1 cut(s) 322
BauI CACGAG 1 cut(s) 176
BbsI GAAGAC 1 cut(s) 22
BbvI GCAGC 4 cut(s) 70, 534, 613, 641
BccI CCATC 3 cut(s) 75, 522, 639
BceAI ACGGC 2 cut(s) 304, 502
BcgI CGANNNNNNTGC 2 cut(s) 133, 167
BciT130I CCWGG 2 cut(s) 73, 818
BclI TGATCA 1 cut(s) 739
BcoDI GTCTC 1 cut(s) 204
BfaI CTAG 1 cut(s) 452
BfmI CTRYAG 1 cut(s) 627
BfuAI ACCTGC 1 cut(s) 610
BisI GCNGC 5 cut(s) 59, 523, 627, 630, 633
BlnI CCTAGG 1 cut(s) 451
BlsI GCNGC 5 cut(s) 60, 524, 628, 631, 634
Bme1390I CCNGG 2 cut(s) 73, 818
BmgBI CACGTC 2 cut(s) 147, 331
BmiI GGNNCC 4 cut(s) 162, 324, 414, 600
BmrFI CCNGG 2 cut(s) 73, 818
BmrI ACTGGG 2 cut(s) 136, 294
BmsI GCATC 1 cut(s) 430
BmuI ACTGGG 2 cut(s) 136, 294
BpiI GAAGAC 1 cut(s) 22
BpuEI CTTGAG 1 cut(s) 685
BsaHI GRCGYC 1 cut(s) 94
BsaJI CCNNGG 5 cut(s) 71, 164, 451, 593, 633
BsaWI WCCGGW 1 cut(s) 421
Bsc4I CCNNNNNNNGG 3 cut(s) 274, 524, 817
Bse118I RCCGGY 1 cut(s) 267
Bse1I ACTGG 2 cut(s) 131, 300
Bse3DI GCAATG 1 cut(s) 512
BseBI CCWGG 2 cut(s) 73, 818
BseDI CCNNGG 5 cut(s) 71, 164, 451, 593, 633
BseGI GGATG 4 cut(s) 67, 487, 533, 650
BseLI CCNNNNNNNGG 3 cut(s) 274, 524, 817
BseMI GCAATG 1 cut(s) 512
BseNI ACTGG 2 cut(s) 131, 300
BseRI GAGGAG 1 cut(s) 551
BseSI GKGCMC 1 cut(s) 280
BseXI GCAGC 4 cut(s) 70, 534, 613, 641
BseYI CCCAGC 1 cut(s) 663
BsgI GTGCAG 2 cut(s) 127, 541
Bsh1236I CGCG 1 cut(s) 635
Bsh1285I CGRYCG 1 cut(s) 85
BshFI GGCC 2 cut(s) 291, 483
BsiEI CGRYCG 1 cut(s) 85
BsiSI CCGG 3 cut(s) 268, 320, 422
BslFI GGGAC 1 cut(s) 651
BslI CCNNNNNNNGG 3 cut(s) 274, 524, 817
BsmAI GTCTC 1 cut(s) 204
BsmFI GGGAC 1 cut(s) 651
BsmI GAATGC 1 cut(s) 248
BsnI GGCC 2 cut(s) 291, 483
Bsp1286I GDGCHC 1 cut(s) 280
Bsp1407I TGTACA 1 cut(s) 204
Bsp143I GATC 6 cut(s) 82, 322, 376, 430, 460, 739
Bsp19I CCATGG 1 cut(s) 593
BspACI CCGC 3 cut(s) 525, 633, 635
BspANI GGCC 2 cut(s) 291, 483
BspFNI CGCG 1 cut(s) 635
BspHI TCATGA 2 cut(s) 97, 457
BspLI GGNNCC 4 cut(s) 162, 324, 414, 600
BspMAI CTGCAG 1 cut(s) 631
BspMI ACCTGC 1 cut(s) 610
BspPI GGATC 3 cut(s) 317, 330, 371
BsrDI GCAATG 1 cut(s) 512
BsrFI RCCGGY 1 cut(s) 267
BsrGI TGTACA 1 cut(s) 204
BsrI ACTGG 2 cut(s) 131, 300
BssAI RCCGGY 1 cut(s) 267
BssECI CCNNGG 5 cut(s) 71, 164, 451, 593, 633
BssMI GATC 6 cut(s) 82, 322, 376, 430, 460, 739
BssNI GRCGYC 1 cut(s) 94
BssSI CACGAG 1 cut(s) 176
BssT1I CCWWGG 2 cut(s) 451, 593
Bst2BI CACGAG 1 cut(s) 176
Bst2UI CCWGG 2 cut(s) 73, 818
Bst4CI ACNGT 2 cut(s) 41, 672
Bst6I CTCTTC 2 cut(s) 237, 543
BstACI GRCGYC 1 cut(s) 94
BstAUI TGTACA 1 cut(s) 204
BstC8I GCNNGC 2 cut(s) 238, 607
BstDEI CTNAG 1 cut(s) 397
BstDSI CCRYGG 3 cut(s) 164, 593, 633
BstEII GGTNACC 1 cut(s) 806
BstF5I GGATG 4 cut(s) 67, 487, 533, 650
BstFNI CGCG 1 cut(s) 635
BstKTI GATC 6 cut(s) 85, 325, 379, 433, 463, 742
BstMAI GTCTC 1 cut(s) 204
BstMBI GATC 6 cut(s) 82, 322, 376, 430, 460, 739
BstMCI CGRYCG 1 cut(s) 85
BstMWI GCNNNNNNNGC 1 cut(s) 632
BstNI CCWGG 2 cut(s) 73, 818
BstNSI RCATGY 1 cut(s) 199
BstPI GGTNACC 1 cut(s) 806
BstSCI CCNGG 2 cut(s) 71, 816
BstSFI CTRYAG 1 cut(s) 627
BstSLI GKGCMC 1 cut(s) 280
BstUI CGCG 1 cut(s) 635
BstV1I GCAGC 4 cut(s) 70, 534, 613, 641
BstV2I GAAGAC 1 cut(s) 22
BstX2I RGATCY 1 cut(s) 322
BstYI RGATCY 1 cut(s) 322
BsuRI GGCC 2 cut(s) 291, 483
BtgI CCRYGG 3 cut(s) 164, 593, 633
BtrI CACGTC 2 cut(s) 147, 331
BtsCI GGATG 4 cut(s) 67, 487, 533, 650
BtsIMutI CAGTG 1 cut(s) 677
BveI ACCTGC 1 cut(s) 610
Cac8I GCNNGC 2 cut(s) 238, 607
CciI TCATGA 2 cut(s) 97, 457
Cfr10I RCCGGY 1 cut(s) 267
Cfr42I CCGCGG 1 cut(s) 636
CseI GACGC 2 cut(s) 50, 83
Csp6I GTAC 3 cut(s) 33, 193, 205
CspCI CAANNNNNGTGG 2 cut(s) 415, 450
CviAII CATG 6 cut(s) 98, 196, 458, 594, 725, 800
CviJI RGCY 9 cut(s) 156, 236, 267, 291, 483, 626, 632, 663, 821
CviKI_1 RGCY 9 cut(s) 156, 236, 267, 291, 483, 626, 632, 663, 821
CviQI GTAC 3 cut(s) 33, 193, 205
DdeI CTNAG 1 cut(s) 397
DpnI GATC 6 cut(s) 84, 324, 378, 432, 462, 741
DpnII GATC 6 cut(s) 82, 322, 376, 430, 460, 739
DrdI GACNNNNNNGTC 1 cut(s) 24
DseDI GACNNNNNNGTC 1 cut(s) 24
Eam1104I CTCTTC 2 cut(s) 237, 543
EarI CTCTTC 2 cut(s) 237, 543
Eco130I CCWWGG 2 cut(s) 451, 593
Eco91I GGTNACC 1 cut(s) 806
EcoO65I GGTNACC 1 cut(s) 806
EcoRII CCWGG 2 cut(s) 71, 816
EcoT14I CCWWGG 2 cut(s) 451, 593
ErhI CCWWGG 2 cut(s) 451, 593
FaeI CATG 6 cut(s) 101, 199, 461, 597, 728, 803
FalI AAGNNNNNCTT 2 cut(s) 106, 138
FaqI GGGAC 1 cut(s) 651
FatI CATG 6 cut(s) 97, 195, 457, 593, 724, 799
FauI CCCGC 1 cut(s) 628
FbaI TGATCA 1 cut(s) 739
Fnu4HI GCNGC 5 cut(s) 59, 523, 627, 630, 633
FokI GGATG 4 cut(s) 54, 474, 540, 657
Fsp4HI GCNGC 5 cut(s) 59, 523, 627, 630, 633
FspBI CTAG 1 cut(s) 452
GluI GCNGC 5 cut(s) 59, 523, 627, 630, 633
GsaI CCCAGC 1 cut(s) 667
HaeIII GGCC 2 cut(s) 291, 483
HapII CCGG 3 cut(s) 268, 320, 422
HgaI GACGC 2 cut(s) 50, 83
Hin1I GRCGYC 1 cut(s) 94
Hin1II CATG 6 cut(s) 101, 199, 461, 597, 728, 803
HincII GTYRAC 1 cut(s) 363
HindII GTYRAC 1 cut(s) 363
HinfI GANTC 3 cut(s) 7, 104, 230
HpaII CCGG 3 cut(s) 268, 320, 422
HphI GGTGA 3 cut(s) 56, 449, 545
Hpy166II GTNNAC 1 cut(s) 363
Hpy188I TCNGA 4 cut(s) 12, 307, 507, 618
Hpy188III TCNNGA 7 cut(s) 98, 326, 458, 464, 584, 702, 717
Hpy8I GTNNAC 1 cut(s) 363
Hpy99I CGWCG 2 cut(s) 155, 332
HpyAV CCTTC 3 cut(s) 220, 494, 774
HpyCH4III ACNGT 2 cut(s) 41, 672
HpyCH4IV ACGT 2 cut(s) 146, 330
HpyCH4V TGCA 3 cut(s) 144, 522, 629
HpyF10VI GCNNNNNNNGC 1 cut(s) 632
HpyF3I CTNAG 1 cut(s) 397
HpySE526I ACGT 2 cut(s) 146, 330
Hsp92I GRCGYC 1 cut(s) 94
Hsp92II CATG 6 cut(s) 101, 199, 461, 597, 728, 803
Ksp22I TGATCA 1 cut(s) 739
KspI CCGCGG 1 cut(s) 636
Kzo9I GATC 6 cut(s) 82, 322, 376, 430, 460, 739
Lsp1109I GCAGC 4 cut(s) 70, 534, 613, 641
LweI GCATC 1 cut(s) 430
MaeI CTAG 1 cut(s) 452
MaeII ACGT 2 cut(s) 146, 330
MaeIII GTNAC 2 cut(s) 62, 806
MalI GATC 6 cut(s) 84, 324, 378, 432, 462, 741
MboI GATC 6 cut(s) 82, 322, 376, 430, 460, 739
MboII GAAGA 4 cut(s) 27, 254, 557, 560
MflI RGATCY 1 cut(s) 322
MhlI GDGCHC 1 cut(s) 280
MluCI AATT 4 cut(s) 137, 294, 372, 691
MlyI GAGTC 3 cut(s) 16, 113, 224
MmeI TCCRAC 1 cut(s) 730
MnlI CCTC 8 cut(s) 238, 390, 529, 544, 635, 746, 756, 767
MslI CAYNNNNRTG 2 cut(s) 462, 517
MspA1I CMGCKG 3 cut(s) 525, 635, 663
MspI CCGG 3 cut(s) 268, 320, 422
MspR9I CCNGG 2 cut(s) 73, 818
Mva1269I GAATGC 1 cut(s) 248
MvaI CCWGG 2 cut(s) 73, 818
MvnI CGCG 1 cut(s) 635
MwoI GCNNNNNNNGC 1 cut(s) 632
NcoI CCATGG 1 cut(s) 593
NdeII GATC 6 cut(s) 82, 322, 376, 430, 460, 739
NlaIII CATG 6 cut(s) 101, 199, 461, 597, 728, 803
NlaIV GGNNCC 4 cut(s) 162, 324, 414, 600
NmeAIII GCCGAG 1 cut(s) 212
NmuCI GTSAC 1 cut(s) 62
NspI RCATGY 1 cut(s) 199
PagI TCATGA 2 cut(s) 97, 457
PciI ACATGT 1 cut(s) 195
PcsI WCGNNNNNNNCGW 2 cut(s) 91, 515
PctI GAATGC 1 cut(s) 248
PflMI CCANNNNNTGG 1 cut(s) 817
PkrI GCNGC 5 cut(s) 60, 524, 628, 631, 634
Ple19I CGATCG 1 cut(s) 85
PleI GAGTC 3 cut(s) 15, 112, 224
PpsI GAGTC 3 cut(s) 15, 112, 224
PscI ACATGT 1 cut(s) 195
PsiI TTATAA 1 cut(s) 735
Psp6I CCWGG 2 cut(s) 71, 816
PspEI GGTNACC 1 cut(s) 806
PspFI CCCAGC 1 cut(s) 663
PspGI CCWGG 2 cut(s) 71, 816
PspN4I GGNNCC 4 cut(s) 162, 324, 414, 600
PstI CTGCAG 1 cut(s) 631
PsuI RGATCY 1 cut(s) 322
PvuI CGATCG 1 cut(s) 85
PvuII CAGCTG 1 cut(s) 663
RsaI GTAC 3 cut(s) 34, 194, 206
RsaNI GTAC 3 cut(s) 33, 193, 205
RseI CAYNNNNRTG 2 cut(s) 462, 517
SacII CCGCGG 1 cut(s) 636
SatI GCNGC 5 cut(s) 59, 523, 627, 630, 633
Sau3AI GATC 6 cut(s) 82, 322, 376, 430, 460, 739
SchI GAGTC 3 cut(s) 16, 113, 224
ScrFI CCNGG 2 cut(s) 73, 818
SduI GDGCHC 1 cut(s) 280
SfaNI GCATC 1 cut(s) 430
SfcI CTRYAG 1 cut(s) 627
Sfr303I CCGCGG 1 cut(s) 636
SgrBI CCGCGG 1 cut(s) 636
SmiMI CAYNNNNRTG 2 cut(s) 462, 517
SmlI CTYRAG 1 cut(s) 700
SmoI CTYRAG 1 cut(s) 700
Sse9I AATT 4 cut(s) 137, 294, 372, 691
SsiI CCGC 3 cut(s) 525, 633, 635
SspI AATATT 1 cut(s) 580
SspMI CTAG 1 cut(s) 452
StyD4I CCNGG 2 cut(s) 71, 816
StyI CCWWGG 2 cut(s) 451, 593
TaaI ACNGT 2 cut(s) 41, 672
TaiI ACGT 2 cut(s) 149, 333
TasI AATT 4 cut(s) 137, 294, 372, 691
TatI WGTACW 2 cut(s) 192, 204
TauI GCSGC 1 cut(s) 635
TscAI CASTG 1 cut(s) 677
TseFI GTSAC 1 cut(s) 62
TseI GCWGC 4 cut(s) 58, 522, 626, 629
Tsp45I GTSAC 1 cut(s) 62
TspDTI ATGAA 4 cut(s) 299, 476, 713, 788
TspGWI ACGGA 2 cut(s) 139, 153
TspRI CASTG 1 cut(s) 677
Van91I CCANNNNNTGG 1 cut(s) 817
XapI RAATTY 1 cut(s) 137
XceI RCATGY 1 cut(s) 199
XmaJI CCTAGG 1 cut(s) 451
XspI CTAG 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.