MD10G1270600.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0014308
Physical Location & Seq
Forward (+)
36284230 .. 36287759
3530 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1270600.v1.1.491

Sequence Viewer

Length: 1101 bp
ATGATCGCGGGAACGGCGAGGAGAATGTGGTTGGTTTCCGTGGTGGTTTTGGCCGTGAGCCTGTGGTGGAGTGGCGCTAGGGCTGAACCGCAAGTGCCTTGCTACTTCATTTTTGGGGACTCTTTGGTTGACAATGGTAATAACAACCAGCTTCAGTCCTTGGCCAGAGCTGACTACTTGCCTTATGGGGTCGACTTTGGTGGACCAACCGGAAGATTTTCCAACGGAAAAACCACCGTTGATGTTGTTGCTGAGCTTCTGGGTTTTGATGATTTCATCCCTCCCTATGCAACTGCTAGAGGCCAAGACGTACTGAAAGGAGTTAACTTTGCATCTGCAGCTGCTGGAATTAGAGAAGAAACTGGACGCCAATTGGGTGATCGGATTACATTTAGTGGTCAGGTAAAGAATTACCAAAACACAGTGTCTCAAGTGGTAAACTTACTAGGTGATGAGGACCAAGCTGCAAATTATCTGAGCAAATGCATATACTCAGTTGGATTAGGCAGCAACGATTACCTTAACAACTATTTTATGCCCCAGTTTTACTCCACCGGCAACCAATTTACCCCGGAGGAATATGCTGATTCGCTCATTCACGATTACGGTCAACAGCTGACGATTTTGTACAATTATGGAGCAAGGAAGGTTGTGTTGTTTGGAATTGGTCAAATAGGTTGCAGTCCAAATGAATTGGCACAAAATAGCCCAGATGGTTCCTCATGCGTGCAAAAAATAAACTCTGCAAACCAAATTTTCAATAGCAAGCTCAAGGCACTTGCCAATGAATTCAACACTAATCTAGCAGATGCAAGATTTATCTTCGTAGACTCTTATGGCATTTTCCAGGACATAATAAGCAACCCTGCACAATATGGATTTAGAGTTACAAACGCAGGGTGCTGTGGGGTAGGGAGGAACAATGGCCAAATTACATGTCTTCCTTTCCAAACTCCATGCCAAAATAGGGATGAGTATCTATTTTGGGATGCATTTCATCCAACTGAGGCTGGCAATGCTGTTATAGCAAGGAGAGCATACAGCGCCGTTCAGGCCTCGGACGCTTACCCTGTCGATATACGCCGGCTTGCCCTTCTTTAA

Protein Analysis

367

Amino Acids

40.13

Weight (kDa)

4.95

Isoelectric Point (pI)

24.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 35 - 345 1.7e-39 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014308)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G29670 AT1G29670 AT5G45670
fragaria_vesca FvH4_3g06610
malus_domestica MD00G1093600.v1.1 MD10G1270600.v1.1
prunus_persica Prupe.4G071100_v2.0.a1
pyrus_communis pycom05g27060 pycom10g22640
rosa_chinensis RchiOBHm_Chr5g0012531
rosa_laevigata RLG00000031922
rosa_multiflora Rmu_sc0001889.1_g000034
rosa_roxburghii Rroxscaffold_1G00064160
rosa_rugosa Rorug05G0002200
rosa_samantha Rh5AG096200 Rh5CG105300
rosa_wichuraiana Rw5G008420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 192, 828
AccII CGCG 1 cut(s) 8
AciI CCGC 2 cut(s) 8, 89
AcoI YGGCCR 3 cut(s) 51, 162, 925
AcsI RAATTY 2 cut(s) 753, 788
AcuI CTGAAG 1 cut(s) 137
AcyI GRCGYC 1 cut(s) 367
AfaI GTAC 2 cut(s) 312, 629
AfiI CCNNNNNNNGG 1 cut(s) 967
AflIII ACRYGT 1 cut(s) 935
AgsI TTSAA 2 cut(s) 760, 793
AjnI CCWGG 1 cut(s) 846
AluBI AGCT 7 cut(s) 151, 170, 256, 341, 464, 616, 769
AluI AGCT 7 cut(s) 151, 170, 256, 341, 464, 616, 769
Alw26I GTCTC 1 cut(s) 432
AlwNI CAGNNNCTG 1 cut(s) 344
AoxI GGCC 5 cut(s) 51, 162, 301, 925, 1053
ApeKI GCWGC 4 cut(s) 338, 341, 464, 507
ApoI RAATTY 2 cut(s) 753, 788
ArsI GACNNNNNNTTYG 2 cut(s) 922, 954
Asp700I GAANNNNTTC 1 cut(s) 217
AspLEI GCGC 2 cut(s) 77, 1046
AspS9I GGNCC 2 cut(s) 203, 457
AsuC2I CCSGG 1 cut(s) 572
AsuHPI GGTGA 2 cut(s) 389, 461
AvaII GGWCC 2 cut(s) 203, 457
BalI TGGCCA 2 cut(s) 164, 927
BbsI GAAGAC 1 cut(s) 932
BbvI GCAGC 4 cut(s) 328, 350, 451, 519
BccI CCATC 1 cut(s) 707
BceAI ACGGC 3 cut(s) 30, 38, 1031
BciT130I CCWGG 1 cut(s) 848
BcnI CCSGG 1 cut(s) 572
BcoDI GTCTC 1 cut(s) 432
BfaI CTAG 4 cut(s) 78, 297, 446, 803
BfmI CTRYAG 1 cut(s) 336
BfoI RGCGCY 2 cut(s) 78, 1047
BglI GCCNNNNNGGC 1 cut(s) 1052
BisI GCNGC 4 cut(s) 339, 342, 465, 508
BlpI GCTNAGC 1 cut(s) 252
BlsI GCNGC 4 cut(s) 340, 343, 466, 509
Bme1390I CCNGG 2 cut(s) 572, 848
Bme18I GGWCC 2 cut(s) 203, 457
BmgT120I GGNCC 2 cut(s) 203, 457
BmiI GGNNCC 1 cut(s) 718
BmrFI CCNGG 2 cut(s) 572, 848
BmrI ACTGGG 1 cut(s) 535
BmsI GCATC 3 cut(s) 341, 799, 979
BmuI ACTGGG 1 cut(s) 535
BpiI GAAGAC 1 cut(s) 932
Bpu1102I GCTNAGC 1 cut(s) 252
BpuEI CTTGAG 2 cut(s) 414, 755
BpuMI CCSGG 1 cut(s) 572
BsaBI GATNNNNATC 1 cut(s) 975
BsaHI GRCGYC 1 cut(s) 367
BsaJI CCNNGG 4 cut(s) 39, 159, 570, 1056
BsaWI WCCGGW 1 cut(s) 209
Bsc4I CCNNNNNNNGG 1 cut(s) 967
Bse118I RCCGGY 2 cut(s) 554, 1083
Bse1I ACTGG 2 cut(s) 367, 541
Bse3DI GCAATG 1 cut(s) 1021
Bse8I GATNNNNATC 1 cut(s) 975
BseBI CCWGG 1 cut(s) 848
BseDI CCNNGG 4 cut(s) 39, 159, 570, 1056
BseGI GGATG 4 cut(s) 276, 976, 994, 997
BseJI GATNNNNATC 1 cut(s) 975
BseLI CCNNNNNNNGG 1 cut(s) 967
BseMI GCAATG 1 cut(s) 1021
BseMII CTCAG 4 cut(s) 243, 467, 507, 996
BseNI ACTGG 2 cut(s) 367, 541
BseRI GAGGAG 1 cut(s) 34
BseXI GCAGC 4 cut(s) 328, 350, 451, 519
BsgI GTGCAG 1 cut(s) 852
Bsh1236I CGCG 1 cut(s) 8
BshFI GGCC 5 cut(s) 53, 164, 303, 927, 1055
BsiSI CCGG 4 cut(s) 210, 555, 572, 1084
BslFI GGGAC 1 cut(s) 131
BslI CCNNNNNNNGG 1 cut(s) 967
BsmAI GTCTC 1 cut(s) 432
BsmFI GGGAC 1 cut(s) 131
BsnI GGCC 5 cut(s) 53, 164, 303, 927, 1055
Bsp1407I TGTACA 1 cut(s) 627
Bsp143I GATC 2 cut(s) 3, 379
Bsp1720I GCTNAGC 1 cut(s) 252
BspACI CCGC 2 cut(s) 8, 89
BspANI GGCC 5 cut(s) 53, 164, 303, 927, 1055
BspCNI CTCAG 4 cut(s) 244, 468, 506, 997
BspFNI CGCG 1 cut(s) 8
BspLI GGNNCC 1 cut(s) 718
BspMAI CTGCAG 1 cut(s) 340
BsrDI GCAATG 1 cut(s) 1021
BsrFI RCCGGY 2 cut(s) 554, 1083
BsrGI TGTACA 1 cut(s) 627
BsrI ACTGG 2 cut(s) 367, 541
BssAI RCCGGY 2 cut(s) 554, 1083
BssECI CCNNGG 4 cut(s) 39, 159, 570, 1056
BssMI GATC 2 cut(s) 3, 379
BssNI GRCGYC 1 cut(s) 367
BssT1I CCWWGG 1 cut(s) 159
Bst2UI CCWGG 1 cut(s) 848
Bst4CI ACNGT 3 cut(s) 238, 424, 608
BstACI GRCGYC 1 cut(s) 367
BstAUI TGTACA 1 cut(s) 627
BstC8I GCNNGC 5 cut(s) 728, 767, 1012, 1085, 1089
BstDEI CTNAG 4 cut(s) 252, 476, 493, 1005
BstDSI CCRYGG 1 cut(s) 39
BstF5I GGATG 4 cut(s) 276, 976, 994, 997
BstFNI CGCG 1 cut(s) 8
BstH2I RGCGCY 2 cut(s) 78, 1047
BstHHI GCGC 2 cut(s) 77, 1046
BstKTI GATC 2 cut(s) 6, 382
BstMAI GTCTC 1 cut(s) 432
BstMBI GATC 2 cut(s) 3, 379
BstMWI GCNNNNNNNGC 8 cut(s) 14, 338, 1016, 1025, 1034, 1043, 1052, 1061
BstNI CCWGG 1 cut(s) 848
BstNSI RCATGY 1 cut(s) 939
BstSCI CCNGG 2 cut(s) 570, 846
BstSFI CTRYAG 1 cut(s) 336
BstUI CGCG 1 cut(s) 8
BstV1I GCAGC 4 cut(s) 328, 350, 451, 519
BstV2I GAAGAC 1 cut(s) 932
BsuRI GGCC 5 cut(s) 53, 164, 303, 927, 1055
BtgI CCRYGG 1 cut(s) 39
BtsCI GGATG 4 cut(s) 276, 976, 994, 997
BtsIMutI CAGTG 1 cut(s) 429
Cac8I GCNNGC 5 cut(s) 728, 767, 1012, 1085, 1089
CaiI CAGNNNCTG 1 cut(s) 344
CfoI GCGC 2 cut(s) 77, 1046
Cfr10I RCCGGY 2 cut(s) 554, 1083
Cfr13I GGNCC 2 cut(s) 203, 457
CseI GACGC 2 cut(s) 375, 1070
Csp6I GTAC 2 cut(s) 311, 628
CviAII CATG 3 cut(s) 723, 936, 957
CviQI GTAC 2 cut(s) 311, 628
DdeI CTNAG 4 cut(s) 252, 476, 493, 1005
DpnI GATC 2 cut(s) 5, 381
DpnII GATC 2 cut(s) 3, 379
EaeI YGGCCR 3 cut(s) 51, 162, 925
Eco130I CCWWGG 1 cut(s) 159
Eco147I AGGCCT 1 cut(s) 1055
Eco47I GGWCC 2 cut(s) 203, 457
Eco57I CTGAAG 1 cut(s) 137
EcoRI GAATTC 1 cut(s) 788
EcoRII CCWGG 1 cut(s) 846
EcoT14I CCWWGG 1 cut(s) 159
EcoT22I ATGCAT 2 cut(s) 488, 994
ErhI CCWWGG 1 cut(s) 159
FaeI CATG 3 cut(s) 726, 939, 960
FaqI GGGAC 1 cut(s) 131
FatI CATG 3 cut(s) 722, 935, 956
FblI GTMKAC 2 cut(s) 192, 828
Fnu4HI GCNGC 4 cut(s) 339, 342, 465, 508
FokI GGATG 4 cut(s) 263, 983, 984, 1001
Fsp4HI GCNGC 4 cut(s) 339, 342, 465, 508
FspBI CTAG 4 cut(s) 78, 297, 446, 803
GlaI GCGC 2 cut(s) 76, 1045
GluI GCNGC 4 cut(s) 339, 342, 465, 508
HaeII RGCGCY 2 cut(s) 78, 1047
HaeIII GGCC 5 cut(s) 53, 164, 303, 927, 1055
HapII CCGG 4 cut(s) 210, 555, 572, 1084
HgaI GACGC 2 cut(s) 375, 1070
HhaI GCGC 2 cut(s) 77, 1046
Hin1I GRCGYC 1 cut(s) 367
Hin1II CATG 3 cut(s) 726, 939, 960
Hin6I GCGC 2 cut(s) 75, 1044
HinP1I GCGC 2 cut(s) 75, 1044
HincII GTYRAC 4 cut(s) 130, 193, 325, 611
HindII GTYRAC 4 cut(s) 130, 193, 325, 611
HinfI GANTC 3 cut(s) 119, 587, 830
HpaI GTTAAC 1 cut(s) 325
HpaII CCGG 4 cut(s) 210, 555, 572, 1084
HphI GGTGA 2 cut(s) 389, 461
Hpy166II GTNNAC 7 cut(s) 130, 193, 203, 325, 439, 611, 829
Hpy188I TCNGA 3 cut(s) 384, 477, 1060
Hpy188III TCNNGA 1 cut(s) 599
Hpy8I GTNNAC 7 cut(s) 130, 193, 203, 325, 439, 611, 829
HpyAV CCTTC 1 cut(s) 640
HpyCH4III ACNGT 3 cut(s) 238, 424, 608
HpyCH4IV ACGT 1 cut(s) 309
HpyF10VI GCNNNNNNNGC 8 cut(s) 14, 338, 1016, 1025, 1034, 1043, 1052, 1061
HpyF3I CTNAG 4 cut(s) 252, 476, 493, 1005
HpySE526I ACGT 1 cut(s) 309
Hsp92I GRCGYC 1 cut(s) 367
Hsp92II CATG 3 cut(s) 726, 939, 960
HspAI GCGC 2 cut(s) 75, 1044
KroI GCCGGC 1 cut(s) 1083
KroNI GCCGGC 1 cut(s) 1085
KspAI GTTAAC 1 cut(s) 325
Kzo9I GATC 2 cut(s) 3, 379
LmnI GCTCC 1 cut(s) 638
Lsp1109I GCAGC 4 cut(s) 328, 350, 451, 519
LweI GCATC 3 cut(s) 341, 799, 979
MaeI CTAG 4 cut(s) 78, 297, 446, 803
MaeII ACGT 1 cut(s) 309
MaeIII GTNAC 1 cut(s) 886
MalI GATC 2 cut(s) 5, 381
MboI GATC 2 cut(s) 3, 379
MboII GAAGA 4 cut(s) 225, 368, 814, 932
MfeI CAATTG 1 cut(s) 371
MlsI TGGCCA 2 cut(s) 164, 927
MluNI TGGCCA 2 cut(s) 164, 927
MlyI GAGTC 2 cut(s) 113, 824
MmeI TCCRAC 3 cut(s) 246, 478, 1025
MnlI CCTC 9 cut(s) 12, 291, 293, 448, 568, 730, 909, 1000, 1066
Mox20I TGGCCA 2 cut(s) 164, 927
Mph1103I ATGCAT 2 cut(s) 488, 994
MroNI GCCGGC 1 cut(s) 1083
MroXI GAANNNNTTC 1 cut(s) 217
MscI TGGCCA 2 cut(s) 164, 927
MseI TTAA 3 cut(s) 324, 522, 1099
Msp20I TGGCCA 2 cut(s) 164, 927
MspA1I CMGCKG 2 cut(s) 341, 616
MspI CCGG 4 cut(s) 210, 555, 572, 1084
MspR9I CCNGG 2 cut(s) 572, 848
MunI CAATTG 1 cut(s) 371
MvaI CCWGG 1 cut(s) 848
MvnI CGCG 1 cut(s) 8
MwoI GCNNNNNNNGC 8 cut(s) 14, 338, 1016, 1025, 1034, 1043, 1052, 1061
NaeI GCCGGC 1 cut(s) 1085
NciI CCSGG 1 cut(s) 572
NdeII GATC 2 cut(s) 3, 379
NgoMIV GCCGGC 1 cut(s) 1083
NlaIII CATG 3 cut(s) 726, 939, 960
NlaIV GGNNCC 1 cut(s) 718
NsiI ATGCAT 2 cut(s) 488, 994
NspI RCATGY 1 cut(s) 939
PceI AGGCCT 1 cut(s) 1055
PciI ACATGT 1 cut(s) 935
PdiI GCCGGC 1 cut(s) 1085
PdmI GAANNNNTTC 1 cut(s) 217
PfeI GAWTC 1 cut(s) 587
PfoI TCCNGGA 1 cut(s) 846
PkrI GCNGC 4 cut(s) 340, 343, 466, 509
PleI GAGTC 2 cut(s) 113, 824
PpsI GAGTC 2 cut(s) 113, 824
PscI ACATGT 1 cut(s) 935
Psp6I CCWGG 1 cut(s) 846
PspGI CCWGG 1 cut(s) 846
PspN4I GGNNCC 1 cut(s) 718
PspPI GGNCC 2 cut(s) 203, 457
PstI CTGCAG 1 cut(s) 340
PstNI CAGNNNCTG 1 cut(s) 344
PvuII CAGCTG 2 cut(s) 341, 616
RsaI GTAC 2 cut(s) 312, 629
RsaNI GTAC 2 cut(s) 311, 628
SalI GTCGAC 1 cut(s) 191
SaqAI TTAA 3 cut(s) 324, 522, 1099
SatI GCNGC 4 cut(s) 339, 342, 465, 508
Sau3AI GATC 2 cut(s) 3, 379
Sau96I GGNCC 2 cut(s) 203, 457
SchI GAGTC 2 cut(s) 113, 824
ScrFI CCNGG 2 cut(s) 572, 848
SfaNI GCATC 3 cut(s) 341, 799, 979
SfcI CTRYAG 1 cut(s) 336
SinI GGWCC 2 cut(s) 203, 457
SmlI CTYRAG 2 cut(s) 429, 770
SmoI CTYRAG 2 cut(s) 429, 770
SseBI AGGCCT 1 cut(s) 1055
SsiI CCGC 2 cut(s) 8, 89
SspMI CTAG 4 cut(s) 78, 297, 446, 803
StuI AGGCCT 1 cut(s) 1055
StyD4I CCNGG 2 cut(s) 570, 846
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 3 cut(s) 238, 424, 608
TaiI ACGT 1 cut(s) 312
TaqI TCGA 2 cut(s) 192, 1074
TatI WGTACW 1 cut(s) 627
TfiI GAWTC 1 cut(s) 587
Tru1I TTAA 3 cut(s) 324, 522, 1099
Tru9I TTAA 3 cut(s) 324, 522, 1099
TscAI CASTG 1 cut(s) 429
TseI GCWGC 4 cut(s) 338, 341, 464, 507
TspDTI ATGAA 5 cut(s) 97, 265, 705, 801, 986
TspGWI ACGGA 2 cut(s) 28, 240
TspRI CASTG 1 cut(s) 429
VpaK11BI GGWCC 2 cut(s) 203, 457
XapI RAATTY 2 cut(s) 753, 788
XceI RCATGY 1 cut(s) 939
XmiI GTMKAC 2 cut(s) 192, 828
XmnI GAANNNNTTC 1 cut(s) 217
XspI CTAG 4 cut(s) 78, 297, 446, 803
Zsp2I ATGCAT 2 cut(s) 488, 994
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.