Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0016045

Physical Location & Seq

Genomic Coordinates Reverse (-)

36783774 .. 36786407

Gene Structure 2634 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1276800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2634 bp

ATGGTGGCCACAACCGCACCGCCAAACTTGTTGTCGATGTCTCCTCTTCCTCGCCACGAAAACAAATCAAAATCTTTCAGACCCTCAAATGGTTACATCTTCCTCAAACCCTCTTCAAAAACCCTTATGTTATCTCCAAATTCGGGCCTTTCTCGTGCCCGATTTCGCGTCTCCGACTCTCTCGCCGCACCCGCCATCACCGGAGTTGCGGACAAGAACGCCAAGATTAACAAGTACTGCGAAATGGGTAATCTGAAATCCGCCATGGAAATGGTGTCCGGGGCTCAAAAATCCGAGCTTGACTTGGAGGCTTACTGCTCCGTCGTGGAGCTATGTGCTGGAATGATGTCGCTGCAAGACGGAAAAGGGGTTCACTCAGTTATCTGTGACAATGGTGTGGAAGCTGATGGGCAACTGGGTGCAAAACTTGTGTTTATGTATGTAAAATGTGGGGATTTAAGAGAAGCGAGGCGGGTTTTCGATAAGTTATCGAATGGGAAGGTGTTTGTTTGGAATCTTATGATTAATGAGTATGCGAAGGTTCGTAATTTCAGGGAAGGTGTGTACTTGTTTGGGAAGATGCAGGAGCTTGGCATCCAAGCAAACTCGTATACGTTTTCATGTGTTTTGAAGTGTTTTTCGGCACTTGGATGTGTGCGGGAAGGTGAATGGATTCATGGATATCTGTATAAATTGGGTTTCGGTTCTGATAACACGGTTGGAAATTCGCTGATGGCTTTCTATTTTAAGAATCGAAGAATTGAGAGTGCACGTAAGGTGTTCGATGAATTGTGCGATCGGGATGTTATATCATGGAATTCGATGATAAGTGCGTATGTGTCTAACGGTCTTGCAGAGAAGGGGGTTGAGATTTTCAGACAGATGGTTTCTTTAGGGATAGATGTGGATTTGGCTACCATTATCAATGTTCTAATGGCTTGTGTAGCCGGTGGGAATCTTTCGCTGGGCAGGGCGCTTCATGCGTATGCAATAAAAGCTTCCTTTGACGAGGATGTCATGTTCTGTAATAATGTTCTGGATATGTATTCGAAATGCGGGGATCTGAGTAGTGCAATCCAGGTTTTTAAGAAGATGGGTGAGAGAAGTGTTGTGTCATGGACTTCAATGATAGCAGGGCACATTCGAGAAGGGCTTTCAGATGAGGCAATCGGATTGTTCTCTGAAATGGAAAGGGACGGTGTTAATCCAGATGCTTATACAATCACGAGCATACTTCATGCATGTGCTAGTAGTGGCTCATTGAAGAAAGGCAGGGATATACACAACTACATTAAGGAACATGGCATGGACTCGAGTTTATATGTCTGTAATACTCTCATGGATATGTATGCAAAATGCGGAAGCATGGAAGATGCTCACTCAGTTTTTTCTCGTATGCCTGCGAAGGACATTGTCTCGTGGAATACCATGATAGGAGGTTACTCAAAAAATTGTCTTCCAAATGAAGCTCTTAAACTCTTTTCCGAGATGATGCAGAAATCAAAACCTGACAGCATGACAATTGCCAGTGTCCTTCCGGCTTGTGCCAGCCTAGCAGCTCTAAATAGAGGTCAAGAGATCCATGGTCATATATTGAGAAATGGATACTTTTCTGAACGGCATGTTGCCAATGCACTTGTCGACATGTATGTCAAGTGTGGGGTGCTAGTTCTTGCACGATTACTTTTTGACATGATTCCCGTAAAAGATCTGATCTCTTGGACTGTGATCATTGCTGGATATGGCATGCATGGATTTGGAAGGGAAGCTATTGCTGCCTTTAATGAGATGAGAAAAGCAGGAATAGAACCCGACAGCGTTTCCTTCATTTCGATACTCTATGCTTGCAGTCATTCGGGATTATGCGATGAAGCATGGAGATTCTTCGATACTATGAGAAATGATTACGGCATTGTTCCCAAGTTAGAGCACTATGCTTGTATGGTGGATCTCCTTTCCCGCACGGGGAATCTAACCAAGGCGTATAAATTTATCAAGACAATGCCGATTGAGCCAGATGCAACAATCTGGGGTTCCTTGCTCTGTGGTTGTAGGATCCACCATGATGTGAAACTAGCGGAGAAAGTCGCTGAACATGTTTTTGAACTAGAGCCCGAAAACACAGGGTATTATGTGCTTCTGGCGAACATATATGCAGAGGCAGAAAAGTGGGAAGAAGTGAAAATGTTGAGGGAGAGGATCACTCGGCGAGGCTTGAAAAAGAATCCAGGCTGTAGTTGGATAGAGATCAAAGGCAAAGTTAAAATCTTTGTCGCTGGAGACAGTTCACACCCACAAGCTGCGAAGATAGAGTCCCTATTGAAGAGATTACGATTGAAGATGAAGGAAGAAGGCCAGTCTCCGAAAATGCAGTATGCTTTGATTAATGCAGATGAGGCGGAGAAAGAAGTGGCCCTCTGTGGGCACAGTGAGAAGTTAGCCATAGCATTTGGGATACTGAACTTGCAACCAGGGAAAACCATACGGGTGACGAAGAATCTACGAGTATGCGGTGACTGTCATGAGATGGCCAAGTTCATCTCCAGGACATCTAGGAGGGAGATTGTTTTAAGAGACTCGAACCGCTTTCACCATATGAAGGACGGGAATTGTTCTTGCAGGGGTTTCTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0006139 GO:0006725 GO:0006807 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009451 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009965 GO:0009987 GO:0010016 GO:0010051 GO:0010087 GO:0010154 GO:0010305 GO:0010588 GO:0016070 GO:0016787 GO:0016788 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0046483 GO:0048316 GO:0048366 GO:0048367 GO:0048598 GO:0048608 GO:0048731 GO:0048825 GO:0048826 GO:0048827 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0090305 GO:0090698 GO:0097159 GO:0099402 GO:1900865 GO:1901360 GO:1901363 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

878

Amino Acids

97.63

Weight (kDa)

8.47

Isoelectric Point (pI)

39.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	168 - 213	1.9e-09	PPR repeat family
PPR_2	PF13041	267 - 307	7e-07	PPR repeat family
PPR	PF01535	270 - 300	3.2e-08	PPR repeat
PPR_2	PF13041	369 - 416	1.2e-13	PPR repeat family
PPR	PF01535	371 - 401	5.4e-08	PPR repeat
PPR_3	PF13812	392 - 450	7.8e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	469 - 516	1e-09	PPR repeat family
PPR	PF01535	472 - 500	3.3e-08	PPR repeat
PPR_2	PF13041	570 - 617	4.1e-09	PPR repeat family
E_motif	PF20431	687 - 749	6.7e-20	E motif
DYW_deaminase	PF14432	785 - 877	5.9e-37	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016045)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G18750
fragaria_vesca	FvH4_3g36560 FvH4_3g36640
malus_domestica	MD10G1276800.v1.1
prunus_persica	Prupe.4G065400_v2.0.a1
pyrus_communis	pycom10g23110
rosa_chinensis	RchiOBHm_Chr5g0011421
rosa_laevigata	RLG00000031832
rosa_multiflora	Rmu_co8262965.1_g000001 Rmu_sc0009975.1_g000017
rosa_roxburghii	Rroxscaffold_1G00065030
rosa_rugosa	Rorug04G0456900
rosa_samantha	Rh5BG083800 Rh5CG097300
rosa_wichuraiana	Rw5G007710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	1013, 1649
AccI	GTMKAC	2 cut(s)	611, 1641
AccII	CGCG	1 cut(s)	168
AclWI	GGATC	6 cut(s)	1068, 1573, 1956, 2050, 2063, 2207
AcoI	YGGCCR	2 cut(s)	6, 2529
AcsI	RAATTY	4 cut(s)	139, 724, 817, 1988
AfaI	GTAC	2 cut(s)	236, 566
AfiI	CCNNNNNNNGG	5 cut(s)	89, 143, 1965, 2421, 2599
AflIII	ACRYGT	2 cut(s)	1644, 2095
AgsI	TTSAA	8 cut(s)	117, 631, 1125, 1264, 2105, 2218, 2323, 2338
AjnI	CCWGG	4 cut(s)	1077, 2227, 2470, 2543
AloI	GAACNNNNNNTCC	4 cut(s)	354, 386, 1004, 1036
AluBI	AGCT	9 cut(s)	298, 331, 404, 589, 998, 1469, 1559, 1769, 2300
AluI	AGCT	9 cut(s)	298, 331, 404, 589, 998, 1469, 1559, 1769, 2300
Alw21I	GWGCWC	2 cut(s)	772, 1932
Alw26I	GTCTC	6 cut(s)	45, 175, 1420, 2274, 2364, 2568
Alw44I	GTGCAC	1 cut(s)	768
AlwI	GGATC	6 cut(s)	1068, 1573, 1956, 2050, 2063, 2207
Ama87I	CYCGRG	1 cut(s)	1312
AoxI	GGCC	5 cut(s)	6, 145, 2353, 2412, 2529
ApaLI	GTGCAC	1 cut(s)	768
ApeKI	GCWGC	4 cut(s)	352, 1556, 1775, 2300
ApoI	RAATTY	4 cut(s)	139, 724, 817, 1988
ArsI	GACNNNNNNTTYG	4 cut(s)	17, 49, 2297, 2329
AseI	ATTAAT	2 cut(s)	525, 2385
Asp700I	GAANNNNTTC	1 cut(s)	672
AspLEI	GCGC	1 cut(s)	976
AspS9I	GGNCC	2 cut(s)	145, 2413
AsuC2I	CCSGG	1 cut(s)	280
AsuHPI	GGTGA	6 cut(s)	190, 677, 1109, 2500, 2525, 2582
AsuII	TTCGAA	1 cut(s)	1049
AvaI	CYCGRG	1 cut(s)	1312
BaeGI	GKGCMC	4 cut(s)	160, 772, 1140, 2427
BalI	TGGCCA	2 cut(s)	8, 2531
BamHI	GGATCC	1 cut(s)	2055
BanII	GRGCYC	2 cut(s)	286, 2115
BauI	CACGAG	3 cut(s)	153, 1225, 1417
BbsI	GAAGAC	1 cut(s)	1448
Bbv12I	GWGCWC	2 cut(s)	772, 1932
BbvI	GCAGC	4 cut(s)	339, 1568, 1762, 2287
BccI	CCATC	6 cut(s)	203, 401, 727, 877, 1087, 2521
BceAI	ACGGC	2 cut(s)	1634, 1924
BciT130I	CCWGG	4 cut(s)	1079, 2229, 2472, 2545
BciVI	GTATCC	2 cut(s)	1598, 2448
BclI	TGATCA	1 cut(s)	1728
BcnI	CCSGG	1 cut(s)	280
BcoDI	GTCTC	6 cut(s)	45, 175, 1420, 2274, 2364, 2568
BfaI	CTAG	6 cut(s)	1248, 1553, 1667, 2075, 2108, 2553
BfmI	CTRYAG	1 cut(s)	2233
BfoI	RGCGCY	1 cut(s)	977
BfuI	GTATCC	2 cut(s)	1598, 2448
BglII	AGATCT	1 cut(s)	1708
BisI	GCNGC	5 cut(s)	186, 353, 1557, 1776, 2301
BlsI	GCNGC	5 cut(s)	187, 354, 1558, 1777, 2302
BmcAI	AGTACT	1 cut(s)	236
Bme1390I	CCNGG	5 cut(s)	280, 1079, 2229, 2472, 2545
BmeT110I	CYCGRG	1 cut(s)	1312
BmgT120I	GGNCC	2 cut(s)	145, 2413
BmiI	GGNNCC	2 cut(s)	2035, 2057
BmrFI	CCNGG	5 cut(s)	280, 1079, 2229, 2472, 2545
BmrI	ACTGGG	1 cut(s)	425
BmsI	GCATC	6 cut(s)	570, 603, 1201, 1363, 1482, 2008
BmuI	ACTGGG	1 cut(s)	425
BpiI	GAAGAC	1 cut(s)	1448
BplI	GAGNNNNNCTC	2 cut(s)	2188, 2220
BpmI	CTGGAG	2 cut(s)	2298, 2527
Bpu14I	TTCGAA	1 cut(s)	1049
BpuMI	CCSGG	1 cut(s)	280
BsaAI	YACGTR	1 cut(s)	773
BsaBI	GATNNNNATC	1 cut(s)	2246
BsaJI	CCNNGG	5 cut(s)	264, 279, 1582, 1977, 2471
BsaWI	WCCGGW	1 cut(s)	200
Bsc4I	CCNNNNNNNGG	5 cut(s)	89, 143, 1965, 2421, 2599
Bse118I	RCCGGY	1 cut(s)	947
Bse1I	ACTGG	3 cut(s)	420, 1527, 2356
Bse3DI	GCAATG	1 cut(s)	1731
Bse8I	GATNNNNATC	1 cut(s)	2246
BseBI	CCWGG	4 cut(s)	1079, 2229, 2472, 2545
BseDI	CCNNGG	5 cut(s)	264, 279, 1582, 1977, 2471
BseGI	GGATG	4 cut(s)	594, 656, 808, 1018
BseJI	GATNNNNATC	1 cut(s)	2246
BseLI	CCNNNNNNNGG	5 cut(s)	89, 143, 1965, 2421, 2599
BseMI	GCAATG	1 cut(s)	1731
BseMII	CTCAG	3 cut(s)	390, 1055, 1395
BseNI	ACTGG	3 cut(s)	420, 1527, 2356
BseRI	GAGGAG	1 cut(s)	33
BseSI	GKGCMC	4 cut(s)	160, 772, 1140, 2427
BseXI	GCAGC	4 cut(s)	339, 1568, 1762, 2287
BseYI	CCCAGC	1 cut(s)	964
Bsh1236I	CGCG	1 cut(s)	168
Bsh1285I	CGRYCG	1 cut(s)	799
BshFI	GGCC	5 cut(s)	8, 147, 2355, 2414, 2531
BsiEI	CGRYCG	1 cut(s)	799
BsiHKAI	GWGCWC	2 cut(s)	772, 1932
BsiHKCI	CYCGRG	1 cut(s)	1312
BsiSI	CCGG	4 cut(s)	201, 279, 948, 1538
BslFI	GGGAC	2 cut(s)	1208, 2299
BslI	CCNNNNNNNGG	5 cut(s)	89, 143, 1965, 2421, 2599
BsmAI	GTCTC	6 cut(s)	45, 175, 1420, 2274, 2364, 2568
BsmBI	CGTCTC	1 cut(s)	175
BsmFI	GGGAC	2 cut(s)	1208, 2299
BsnI	GGCC	5 cut(s)	8, 147, 2355, 2414, 2531
BsoBI	CYCGRG	1 cut(s)	1312
Bsp119I	TTCGAA	1 cut(s)	1049
Bsp1286I	GDGCHC	7 cut(s)	160, 286, 772, 1140, 1932, 2115, 2427
Bsp19I	CCATGG	2 cut(s)	264, 1582
BspANI	GGCC	5 cut(s)	8, 147, 2355, 2414, 2531
BspCNI	CTCAG	3 cut(s)	389, 1056, 1394
BspFNI	CGCG	1 cut(s)	168
BspHI	TCATGA	1 cut(s)	2521
BspLI	GGNNCC	2 cut(s)	2035, 2057
BspPI	GGATC	6 cut(s)	1068, 1573, 1956, 2050, 2063, 2207
BspT104I	TTCGAA	1 cut(s)	1049
BsrDI	GCAATG	1 cut(s)	1731
BsrFI	RCCGGY	1 cut(s)	947
BsrI	ACTGG	3 cut(s)	420, 1527, 2356
BssAI	RCCGGY	1 cut(s)	947
BssECI	CCNNGG	5 cut(s)	264, 279, 1582, 1977, 2471
BssNAI	GTATAC	1 cut(s)	612
BssSI	CACGAG	3 cut(s)	153, 1225, 1417
BssT1I	CCWWGG	3 cut(s)	264, 1582, 1977
Bst1107I	GTATAC	1 cut(s)	612
Bst2BI	CACGAG	3 cut(s)	153, 1225, 1417
Bst2UI	CCWGG	4 cut(s)	1079, 2229, 2472, 2545
Bst4CI	ACNGT	7 cut(s)	718, 848, 1199, 1726, 2285, 2429, 2519
Bst6I	CTCTTC	3 cut(s)	51, 118, 2318
BstBAI	YACGTR	1 cut(s)	773
BstBI	TTCGAA	1 cut(s)	1049
BstC8I	GCNNGC	4 cut(s)	1401, 1549, 1748, 1846
BstDEI	CTNAG	3 cut(s)	376, 1064, 1381
BstDSI	CCRYGG	2 cut(s)	264, 1582
BstF5I	GGATG	4 cut(s)	594, 656, 808, 1018
BstFNI	CGCG	1 cut(s)	168
BstH2I	RGCGCY	1 cut(s)	977
BstHHI	GCGC	1 cut(s)	976
BstMAI	GTCTC	6 cut(s)	45, 175, 1420, 2274, 2364, 2568
BstMCI	CGRYCG	1 cut(s)	799
BstMWI	GCNNNNNNNGC	9 cut(s)	14, 191, 944, 980, 995, 1553, 1775, 2011, 2396
BstNI	CCWGG	4 cut(s)	1079, 2229, 2472, 2545
BstNSI	RCATGY	5 cut(s)	1245, 1625, 1648, 1750, 2099
BstSCI	CCNGG	5 cut(s)	278, 1077, 2227, 2470, 2543
BstSFI	CTRYAG	1 cut(s)	2233
BstSLI	GKGCMC	4 cut(s)	160, 772, 1140, 2427
BstUI	CGCG	1 cut(s)	168
BstV1I	GCAGC	4 cut(s)	339, 1568, 1762, 2287
BstV2I	GAAGAC	1 cut(s)	1448
BstX2I	RGATCY	5 cut(s)	1060, 1578, 1708, 1948, 2055
BstXI	CCANNNNNNTGG	1 cut(s)	271
BstYI	RGATCY	5 cut(s)	1060, 1578, 1708, 1948, 2055
BstZ17I	GTATAC	1 cut(s)	612
BsuI	GTATCC	2 cut(s)	1598, 2448
BsuRI	GGCC	5 cut(s)	8, 147, 2355, 2414, 2531
BtgI	CCRYGG	2 cut(s)	264, 1582
BtgZI	GCGATG	1 cut(s)	1881
BtsCI	GGATG	4 cut(s)	594, 656, 808, 1018
BtsIMutI	CAGTG	2 cut(s)	1534, 2434
Cac8I	GCNNGC	4 cut(s)	1401, 1549, 1748, 1846
CciI	TCATGA	1 cut(s)	2521
CfoI	GCGC	1 cut(s)	976
Cfr10I	RCCGGY	1 cut(s)	947
Cfr13I	GGNCC	2 cut(s)	145, 2413
CseI	GACGC	1 cut(s)	157
Csp6I	GTAC	2 cut(s)	235, 565
CviQI	GTAC	2 cut(s)	235, 565
DdeI	CTNAG	3 cut(s)	376, 1064, 1381
DrdI	GACNNNNNNGTC	2 cut(s)	1013, 1649
DseDI	GACNNNNNNGTC	2 cut(s)	1013, 1649
EaeI	YGGCCR	2 cut(s)	6, 2529
Eam1104I	CTCTTC	3 cut(s)	51, 118, 2318
EarI	CTCTTC	3 cut(s)	51, 118, 2318
EciI	GGCGGA	2 cut(s)	250, 2414
Eco130I	CCWWGG	3 cut(s)	264, 1582, 1977
Eco24I	GRGCYC	2 cut(s)	286, 2115
Eco32I	GATATC	1 cut(s)	683
Eco88I	CYCGRG	1 cut(s)	1312
EcoRI	GAATTC	1 cut(s)	817
EcoRII	CCWGG	4 cut(s)	1077, 2227, 2470, 2543
EcoRV	GATATC	1 cut(s)	683
EcoT14I	CCWWGG	3 cut(s)	264, 1582, 1977
EcoT22I	ATGCAT	2 cut(s)	1243, 1752
EcoT38I	GRGCYC	2 cut(s)	286, 2115
ErhI	CCWWGG	3 cut(s)	264, 1582, 1977
Esp3I	CGTCTC	1 cut(s)	175
FaqI	GGGAC	2 cut(s)	1208, 2299
FauI	CCCGC	5 cut(s)	199, 465, 651, 1049, 1967
FauNDI	CATATG	1 cut(s)	2595
FbaI	TGATCA	1 cut(s)	1728
FblI	GTMKAC	2 cut(s)	611, 1641
Fnu4HI	GCNGC	5 cut(s)	186, 353, 1557, 1776, 2301
FokI	GGATG	4 cut(s)	581, 663, 815, 1025
FriOI	GRGCYC	2 cut(s)	286, 2115
Fsp4HI	GCNGC	5 cut(s)	186, 353, 1557, 1776, 2301
FspBI	CTAG	6 cut(s)	1248, 1553, 1667, 2075, 2108, 2553
GlaI	GCGC	1 cut(s)	975
GluI	GCNGC	5 cut(s)	186, 353, 1557, 1776, 2301
GsaI	CCCAGC	1 cut(s)	968
GsuI	CTGGAG	2 cut(s)	2298, 2527
HaeII	RGCGCY	1 cut(s)	977
HaeIII	GGCC	5 cut(s)	8, 147, 2355, 2414, 2531
HapII	CCGG	4 cut(s)	201, 279, 948, 1538
HgaI	GACGC	1 cut(s)	157
HhaI	GCGC	1 cut(s)	976
Hin6I	GCGC	1 cut(s)	974
HinP1I	GCGC	1 cut(s)	974
HincII	GTYRAC	1 cut(s)	1642
HindII	GTYRAC	1 cut(s)	1642
HindIII	AAGCTT	1 cut(s)	996
HpaII	CCGG	4 cut(s)	201, 279, 948, 1538
HphI	GGTGA	6 cut(s)	190, 677, 1109, 2500, 2525, 2582
Hpy166II	GTNNAC	6 cut(s)	373, 565, 612, 770, 1642, 2288
Hpy188III	TCNNGA	9 cut(s)	800, 1037, 1145, 1208, 1225, 1574, 1857, 1996, 2522
Hpy8I	GTNNAC	6 cut(s)	373, 565, 612, 770, 1642, 2288
Hpy99I	CGWCG	1 cut(s)	326
HpyCH4III	ACNGT	7 cut(s)	718, 848, 1199, 1726, 2285, 2429, 2519
HpyCH4IV	ACGT	2 cut(s)	614, 772
HpyF10VI	GCNNNNNNNGC	9 cut(s)	14, 191, 944, 980, 995, 1553, 1775, 2011, 2396
HpyF3I	CTNAG	3 cut(s)	376, 1064, 1381
HpySE526I	ACGT	2 cut(s)	614, 772
HspAI	GCGC	1 cut(s)	974
Ksp22I	TGATCA	1 cut(s)	1728
LmnI	GCTCC	3 cut(s)	323, 328, 586
Lsp1109I	GCAGC	4 cut(s)	339, 1568, 1762, 2287
LweI	GCATC	6 cut(s)	570, 603, 1201, 1363, 1482, 2008
MaeI	CTAG	6 cut(s)	1248, 1553, 1667, 2075, 2108, 2553
MaeII	ACGT	2 cut(s)	614, 772
MaeIII	GTNAC	5 cut(s)	92, 386, 1439, 2488, 2513
MfeI	CAATTG	1 cut(s)	1521
MflI	RGATCY	5 cut(s)	1060, 1578, 1708, 1948, 2055
MhlI	GDGCHC	7 cut(s)	160, 286, 772, 1140, 1932, 2115, 2427
MlsI	TGGCCA	2 cut(s)	8, 2531
MluNI	TGGCCA	2 cut(s)	8, 2531
MlyI	GAGTC	4 cut(s)	170, 1304, 2321, 2570
MmeI	TCCRAC	3 cut(s)	198, 700, 2219
Mox20I	TGGCCA	2 cut(s)	8, 2531
Mph1103I	ATGCAT	2 cut(s)	1243, 1752
MroXI	GAANNNNTTC	1 cut(s)	672
MscI	TGGCCA	2 cut(s)	8, 2531
MslI	CAYNNNNRTG	7 cut(s)	269, 649, 984, 1242, 1343, 2064, 2486
Msp20I	TGGCCA	2 cut(s)	8, 2531
MspI	CCGG	4 cut(s)	201, 279, 948, 1538
MspR9I	CCNGG	5 cut(s)	280, 1079, 2229, 2472, 2545
MunI	CAATTG	1 cut(s)	1521
MvaI	CCWGG	4 cut(s)	1079, 2229, 2472, 2545
MvnI	CGCG	1 cut(s)	168
MwoI	GCNNNNNNNGC	9 cut(s)	14, 191, 944, 980, 995, 1553, 1775, 2011, 2396
NciI	CCSGG	1 cut(s)	280
NcoI	CCATGG	2 cut(s)	264, 1582
NdeI	CATATG	1 cut(s)	2595
NlaIV	GGNNCC	2 cut(s)	2035, 2057
NmeAIII	GCCGAG	1 cut(s)	2185
NmuCI	GTSAC	3 cut(s)	386, 2488, 2513
NsiI	ATGCAT	2 cut(s)	1243, 1752
NspI	RCATGY	5 cut(s)	1245, 1625, 1648, 1750, 2099
NspV	TTCGAA	1 cut(s)	1049
PaeI	GCATGC	1 cut(s)	1750
PaeR7I	CTCGAG	1 cut(s)	1312
PagI	TCATGA	1 cut(s)	2521
PciI	ACATGT	2 cut(s)	1644, 2095
PdmI	GAANNNNTTC	1 cut(s)	672
PfeI	GAWTC	9 cut(s)	514, 673, 751, 955, 1696, 1881, 1969, 2224, 2497
PflFI	GACNNNGTC	1 cut(s)	1412
PfoI	TCCNGGA	1 cut(s)	2543
PkrI	GCNGC	5 cut(s)	187, 354, 1558, 1777, 2302
Ple19I	CGATCG	1 cut(s)	799
PleI	GAGTC	4 cut(s)	170, 1304, 2320, 2570
PpsI	GAGTC	4 cut(s)	170, 1304, 2320, 2570
Ppu21I	YACGTR	1 cut(s)	773
PscI	ACATGT	2 cut(s)	1644, 2095
PshBI	ATTAAT	2 cut(s)	525, 2385
Psp6I	CCWGG	4 cut(s)	1077, 2227, 2470, 2543
PspFI	CCCAGC	1 cut(s)	964
PspGI	CCWGG	4 cut(s)	1077, 2227, 2470, 2543
PspN4I	GGNNCC	2 cut(s)	2035, 2057
PspPI	GGNCC	2 cut(s)	145, 2413
PspXI	VCTCGAGB	1 cut(s)	1312
PsuI	RGATCY	5 cut(s)	1060, 1578, 1708, 1948, 2055
PsyI	GACNNNGTC	1 cut(s)	1412
PvuI	CGATCG	1 cut(s)	799
RsaI	GTAC	2 cut(s)	236, 566
RsaNI	GTAC	2 cut(s)	235, 565
RseI	CAYNNNNRTG	7 cut(s)	269, 649, 984, 1242, 1343, 2064, 2486
SalI	GTCGAC	1 cut(s)	1640
SatI	GCNGC	5 cut(s)	186, 353, 1557, 1776, 2301
Sau96I	GGNCC	2 cut(s)	145, 2413
ScaI	AGTACT	1 cut(s)	236
SchI	GAGTC	4 cut(s)	170, 1304, 2321, 2570
ScrFI	CCNGG	5 cut(s)	280, 1079, 2229, 2472, 2545
SduI	GDGCHC	7 cut(s)	160, 286, 772, 1140, 1932, 2115, 2427
SfaNI	GCATC	6 cut(s)	570, 603, 1201, 1363, 1482, 2008
SfcI	CTRYAG	1 cut(s)	2233
Sfr274I	CTCGAG	1 cut(s)	1312
SfuI	TTCGAA	1 cut(s)	1049
SlaI	CTCGAG	1 cut(s)	1312
SmiMI	CAYNNNNRTG	7 cut(s)	269, 649, 984, 1242, 1343, 2064, 2486
SmlI	CTYRAG	1 cut(s)	1312
SmoI	CTYRAG	1 cut(s)	1312
SphI	GCATGC	1 cut(s)	1750
SspMI	CTAG	6 cut(s)	1248, 1553, 1667, 2075, 2108, 2553
StyD4I	CCNGG	5 cut(s)	278, 1077, 2227, 2470, 2543
StyI	CCWWGG	3 cut(s)	264, 1582, 1977
TaaI	ACNGT	7 cut(s)	718, 848, 1199, 1726, 2285, 2429, 2519
TaiI	ACGT	2 cut(s)	617, 775
TatI	WGTACW	2 cut(s)	234, 564
TauI	GCSGC	1 cut(s)	188
TfiI	GAWTC	9 cut(s)	514, 673, 751, 955, 1696, 1881, 1969, 2224, 2497
TscAI	CASTG	2 cut(s)	1534, 2434
TseFI	GTSAC	3 cut(s)	386, 2488, 2513
TseI	GCWGC	4 cut(s)	352, 1556, 1775, 2300
Tsp45I	GTSAC	3 cut(s)	386, 2488, 2513
TspGWI	ACGGA	2 cut(s)	310, 375
TspRI	CASTG	2 cut(s)	1534, 2434
Tth111I	GACNNNGTC	1 cut(s)	1412
VneI	GTGCAC	1 cut(s)	768
VspI	ATTAAT	2 cut(s)	525, 2385
XapI	RAATTY	4 cut(s)	139, 724, 817, 1988
XceI	RCATGY	5 cut(s)	1245, 1625, 1648, 1750, 2099
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2235
XhoI	CTCGAG	1 cut(s)	1312
XmiI	GTMKAC	2 cut(s)	611, 1641
XmnI	GAANNNNTTC	1 cut(s)	672
XspI	CTAG	6 cut(s)	1248, 1553, 1667, 2075, 2108, 2553
ZrmI	AGTACT	1 cut(s)	236
Zsp2I	ATGCAT	2 cut(s)	1243, 1752

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.