Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0016045

Physical Location & Seq

Genomic Coordinates Reverse (-)

25356033 .. 25358672

Gene Structure 2640 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom10g23110.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2640 bp

ATGTTGATGGTGGCCACAACCGCACCGCCAAACTTGTTGTCGATGTCTCCTCTTCCTCGCCACCAAAACAAATCAAAATCTTTCAGACCCTCAAATGGTTACATCTTCCTCAAACCCTCTTCAAAAACCCCTATGTTATCTCCAAATTCGGGCCTTTCTCGTGCCCGATTTCGCGTCTCCGACTCTCTCGCCGCACCCTCCATCACCAGAGTTGCAGACAAGAACGCCAAGATTAACAAGTACTGTGAAATGGATAATCTGAAATCCGCCATGGAAATGGTGTCCGGGGCTCAAAAATTCGAGCTTGACTTGGAGGCTTACTGCTCCGTTGTGGAGCTATGTGCTGGAATGATGTCGCTTCAAGACGGAAAAAGGGTTCACTCAGTTATCTGTGACAATGGTGTGGAAGCTGATGGGCAACTGGGTGCAAAACTTGTGTTTATGTATGTAAAATGTGGGGATTTAAGAGAAGCGAGGCGGGTTTTCGATAAGTTATCGAATGGGAAGGTGTTTGTTTGGAATCTTATGATTAATGAGTATGCGAAGGTTCGTAATTTCAGGGAAGGTGTGTACTTGTTTGGGAAGATGCAGGAGCTTGGCATCCAAGCAAACTCGTATACGTTTTCATGTGTTTTGAAGTGTTTTTCGGCACTTGGATGTGTGCGGGAAGGCGAATGGATTCATGGATATCTGTATAAATTGGGTTTCGGTTCTGATAACACGGTTGGAAATTCGCTGATGGCTTTCTATTTTAAGAATCGAAGAATTGAGAGTGCACGTAAGGTGTTCGATGAATTGTACGATCGGGATGTTATATCATGGAATTCGATGATAAGTGCGTATGTGTCTAACGGTCTTGCAGAGAAGGGGGTTGAGATTTTCAGACAGATGGTTTCTTTAGGGATAGATGTGGATTTGGCTACAATTATCAATGTTCTAATGGCTTGTGTAGCCGGTGGGAATCTTTCGCTGGGCAGGGCGCTTCATGCATATGCAATAAAAGCTTCTTTTGACGAGGATGTCATGTTCTGTAATAATGTTCTAGATATGTATTCGAAATGCGGGGATCTGAGTAGTGCAATCCAGGTTTTTAAGAAGATGGGTGAGAGAAGTGTTGTGTCATGGACTTCAATGATAGCAGGGCACATTCGAGAAGGGCTTTCAGATGAGGCAATCAGATTGTTCTCTGAAATGGAAAGGGACGGTGTTAATCCAGATGCTTATACAATCACGAGCATACTTCATGCATGTGCTAGTAGTGGCTCATTGAAGAAAGGCAGGGATATACACAACTACATTAAGGAACATGGTATGGACTCGAGTTTATATGTCTGTAATACTCTCATGGATATGTATGCAAAATGCGGAAGCATGGAAGATGCTCACTCAGTTTTTTCTCGTATGCCTGCGAAGGACATTGTCTCGTGGAATACCATGATAGGAGGTTACTCAAAAAATTGTCTTCCAAATGAAGCTCTTAAACTCTTTTCTGAGATGATGCAAAAATCAAAACCTGACAGCATGACAATTGCCAGTATCCTTCCGGCTTGTGCCAGCCTAGCAGCTCTAAATAGAGGTCAAGAGATCCATGGTCATATATTGAGAAATGGATACTTTTCTGAACGGCATGTTGCCAATGCACTTGTCGACATGTATGTCAAGTGCGGGGTACTAGTTCTTGCACGATTACTTTTTGACATGATTCCCGTAAAAGATCTGATCTCTTGGACTGTGATCGTTGCTGGATATGGCATGCATGGATTTGGAAGGGAAGCTATTGCTGCCTTTAATGAGATGAGAAAAGCAGGAACAGAACCCGACAGCGTTTCCTTCATTTCAATACTCTATGCTTGCAGTCATTCGGGATTATGCGATGAAGCATGGAGATTCTTCAATACTATGAGAAATGATTACGGCATTGTTCCCAAGTTAGAGCACTATGCTTGTATGGTGGATCTCCTTTCCCGCACGGGGAATCTAACCAAGGCGTATAAGTTTATCAAGACGATGCCGATTGAGCCAGATGCAACAATCTGGGGTTCCTTGCTCTGTGGTTGTAGGATCCACCATGACGTGAAACTAGCGGAGAAAGTCGCCGAACATGTTTTTGAACTAGAGCCCGAAAACACAGGGTATTATGTACTTCTGGCGAACATATATGCAGAGGCAGAAAAGTGGGAAGAAGTGAAAAAGTTGAGGGAGAGGATCACTCGGCGAGGCTTGAAAAAGAATCCAGGCTGTAGTTGGATAGAGATCAAAGGCAAAGTTAAAATCTTTGTCGCTGGAGACAGTTCACAGCCACAAGCTGCAAAGATAGAGTCCTTATTGAAGAGATTACGATTGAAGATGAAGGAAGAAGGCCAGTCTCCGAAAATGCAGTATGCTTTGATTAATGCAGATGAGGCGGAGAAAGAGGTGGCCCTCTGTGGGCACAGTGAGAAGTTAGCCATAGCATTTGGGATACTGAACTTGCAACCAGGGAAAACCATACGGGTGACGAAGAACCTACGAGTATGCGGTGACTGTCATGAGATGGCCAAGTTCATCTCCAGGACATCTAGGAGGGAGATTGTTTTAAGAGACTCAAACCGCTTTCACCATATGAAGGAGGGGAATTGTTCTTGCAGGGGTTTCTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0006139 GO:0006725 GO:0006807 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009451 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009965 GO:0009987 GO:0010016 GO:0010051 GO:0010087 GO:0010154 GO:0010305 GO:0010588 GO:0016070 GO:0016787 GO:0016788 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0046483 GO:0048316 GO:0048366 GO:0048367 GO:0048598 GO:0048608 GO:0048731 GO:0048825 GO:0048826 GO:0048827 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0090305 GO:0090698 GO:0097159 GO:0099402 GO:1900865 GO:1901360 GO:1901363 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

880

Amino Acids

98.34

Weight (kDa)

8.71

Isoelectric Point (pI)

39.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	170 - 215	1.9e-09	PPR repeat family
PPR_2	PF13041	269 - 309	7e-07	PPR repeat family
PPR	PF01535	272 - 302	3.2e-08	PPR repeat
TPR_24	PF23276	343 - 467	9e-06	Fungal tetratrico peptide repeats
PPR_2	PF13041	371 - 418	2.4e-14	PPR repeat family
PPR	PF01535	373 - 403	4e-08	PPR repeat
PPR_3	PF13812	393 - 453	1.6e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	471 - 518	7.5e-10	PPR repeat family
PPR	PF01535	474 - 502	3.3e-08	PPR repeat
PPR_2	PF13041	572 - 619	1.9e-07	PPR repeat family
E_motif	PF20431	689 - 751	1.3e-20	E motif
DYW_deaminase	PF14432	787 - 879	1.1e-36	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0016045)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G18750
fragaria_vesca	FvH4_3g36560 FvH4_3g36640
malus_domestica	MD10G1276800.v1.1
prunus_persica	Prupe.4G065400_v2.0.a1
pyrus_communis	pycom10g23110
rosa_chinensis	RchiOBHm_Chr5g0011421
rosa_laevigata	RLG00000031832
rosa_multiflora	Rmu_co8262965.1_g000001 Rmu_sc0009975.1_g000017
rosa_roxburghii	Rroxscaffold_1G00065030
rosa_rugosa	Rorug04G0456900
rosa_samantha	Rh5BG083800 Rh5CG097300
rosa_wichuraiana	Rw5G007710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	1019, 1655
AccI	GTMKAC	2 cut(s)	617, 1647
AccII	CGCG	1 cut(s)	174
AclWI	GGATC	6 cut(s)	1074, 1579, 1962, 2056, 2069, 2213
AcoI	YGGCCR	2 cut(s)	12, 2535
AcsI	RAATTY	4 cut(s)	145, 296, 730, 823
AfaI	GTAC	5 cut(s)	242, 572, 800, 1671, 2142
AfiI	CCNNNNNNNGG	5 cut(s)	95, 149, 1971, 2427, 2605
AflIII	ACRYGT	2 cut(s)	1650, 2101
AhlI	ACTAGT	1 cut(s)	1672
AjiI	CACGTC	1 cut(s)	2074
AjnI	CCWGG	4 cut(s)	1083, 2233, 2476, 2549
AloI	GAACNNNNNNTCC	4 cut(s)	360, 392, 1010, 1042
AluBI	AGCT	9 cut(s)	304, 337, 410, 595, 1004, 1475, 1565, 1775, 2306
AluI	AGCT	9 cut(s)	304, 337, 410, 595, 1004, 1475, 1565, 1775, 2306
Alw21I	GWGCWC	2 cut(s)	778, 1938
Alw26I	GTCTC	6 cut(s)	51, 181, 1426, 2280, 2370, 2574
Alw44I	GTGCAC	1 cut(s)	774
AlwI	GGATC	6 cut(s)	1074, 1579, 1962, 2056, 2069, 2213
Ama87I	CYCGRG	1 cut(s)	1318
AoxI	GGCC	5 cut(s)	12, 151, 2359, 2418, 2535
ApaLI	GTGCAC	1 cut(s)	774
ApeKI	GCWGC	3 cut(s)	1562, 1781, 2306
ApoI	RAATTY	4 cut(s)	145, 296, 730, 823
ArsI	GACNNNNNNTTYG	4 cut(s)	23, 55, 2303, 2335
AseI	ATTAAT	2 cut(s)	531, 2391
Asp700I	GAANNNNTTC	1 cut(s)	678
AspLEI	GCGC	1 cut(s)	982
AspS9I	GGNCC	2 cut(s)	151, 2419
AsuC2I	CCSGG	1 cut(s)	286
AsuHPI	GGTGA	5 cut(s)	196, 1115, 2506, 2531, 2588
AsuII	TTCGAA	1 cut(s)	1055
AvaI	CYCGRG	1 cut(s)	1318
BaeGI	GKGCMC	4 cut(s)	166, 778, 1146, 2433
BalI	TGGCCA	2 cut(s)	14, 2537
BamHI	GGATCC	1 cut(s)	2061
BanII	GRGCYC	2 cut(s)	292, 2121
BauI	CACGAG	3 cut(s)	159, 1231, 1423
BbsI	GAAGAC	1 cut(s)	1454
Bbv12I	GWGCWC	2 cut(s)	778, 1938
BbvI	GCAGC	3 cut(s)	1574, 1768, 2293
BccI	CCATC	6 cut(s)	209, 407, 733, 883, 1093, 2527
BceAI	ACGGC	2 cut(s)	1640, 1930
BciT130I	CCWGG	4 cut(s)	1085, 2235, 2478, 2551
BciVI	GTATCC	3 cut(s)	1547, 1604, 2454
BcnI	CCSGG	1 cut(s)	286
BcoDI	GTCTC	6 cut(s)	51, 181, 1426, 2280, 2370, 2574
BcuI	ACTAGT	1 cut(s)	1672
BfaI	CTAG	7 cut(s)	1043, 1254, 1559, 1673, 2081, 2114, 2559
BfmI	CTRYAG	1 cut(s)	2239
BfoI	RGCGCY	1 cut(s)	983
BfuI	GTATCC	3 cut(s)	1547, 1604, 2454
BglII	AGATCT	1 cut(s)	1714
BisI	GCNGC	4 cut(s)	192, 1563, 1782, 2307
BlsI	GCNGC	4 cut(s)	193, 1564, 1783, 2308
BmcAI	AGTACT	1 cut(s)	242
Bme1390I	CCNGG	5 cut(s)	286, 1085, 2235, 2478, 2551
BmeT110I	CYCGRG	1 cut(s)	1318
BmgBI	CACGTC	1 cut(s)	2074
BmgT120I	GGNCC	2 cut(s)	151, 2419
BmiI	GGNNCC	2 cut(s)	2041, 2063
BmrFI	CCNGG	5 cut(s)	286, 1085, 2235, 2478, 2551
BmrI	ACTGGG	1 cut(s)	431
BmsI	GCATC	7 cut(s)	576, 609, 1207, 1369, 1488, 1998, 2014
BmuI	ACTGGG	1 cut(s)	431
BpiI	GAAGAC	1 cut(s)	1454
BplI	GAGNNNNNCTC	2 cut(s)	2194, 2226
BpmI	CTGGAG	2 cut(s)	2304, 2533
Bpu14I	TTCGAA	1 cut(s)	1055
BpuMI	CCSGG	1 cut(s)	286
BsaAI	YACGTR	1 cut(s)	779
BsaBI	GATNNNNATC	1 cut(s)	2252
BsaJI	CCNNGG	5 cut(s)	270, 285, 1588, 1983, 2477
Bsc4I	CCNNNNNNNGG	5 cut(s)	95, 149, 1971, 2427, 2605
Bse118I	RCCGGY	1 cut(s)	953
Bse1I	ACTGG	3 cut(s)	426, 1533, 2362
Bse8I	GATNNNNATC	1 cut(s)	2252
BseBI	CCWGG	4 cut(s)	1085, 2235, 2478, 2551
BseDI	CCNNGG	5 cut(s)	270, 285, 1588, 1983, 2477
BseGI	GGATG	4 cut(s)	600, 662, 814, 1024
BseJI	GATNNNNATC	1 cut(s)	2252
BseLI	CCNNNNNNNGG	5 cut(s)	95, 149, 1971, 2427, 2605
BseMII	CTCAG	4 cut(s)	396, 1061, 1401, 1482
BseNI	ACTGG	3 cut(s)	426, 1533, 2362
BseRI	GAGGAG	1 cut(s)	39
BseSI	GKGCMC	4 cut(s)	166, 778, 1146, 2433
BseXI	GCAGC	3 cut(s)	1574, 1768, 2293
BseYI	CCCAGC	1 cut(s)	970
Bsh1236I	CGCG	1 cut(s)	174
Bsh1285I	CGRYCG	1 cut(s)	805
BshFI	GGCC	5 cut(s)	14, 153, 2361, 2420, 2537
BsiEI	CGRYCG	1 cut(s)	805
BsiHKAI	GWGCWC	2 cut(s)	778, 1938
BsiHKCI	CYCGRG	1 cut(s)	1318
BsiSI	CCGG	3 cut(s)	285, 954, 1544
BslFI	GGGAC	1 cut(s)	1214
BslI	CCNNNNNNNGG	5 cut(s)	95, 149, 1971, 2427, 2605
BsmAI	GTCTC	6 cut(s)	51, 181, 1426, 2280, 2370, 2574
BsmBI	CGTCTC	1 cut(s)	181
BsmFI	GGGAC	1 cut(s)	1214
BsnI	GGCC	5 cut(s)	14, 153, 2361, 2420, 2537
BsoBI	CYCGRG	1 cut(s)	1318
Bsp119I	TTCGAA	1 cut(s)	1055
Bsp1286I	GDGCHC	7 cut(s)	166, 292, 778, 1146, 1938, 2121, 2433
Bsp19I	CCATGG	2 cut(s)	270, 1588
BspANI	GGCC	5 cut(s)	14, 153, 2361, 2420, 2537
BspCNI	CTCAG	4 cut(s)	395, 1062, 1400, 1483
BspFNI	CGCG	1 cut(s)	174
BspHI	TCATGA	1 cut(s)	2527
BspLI	GGNNCC	2 cut(s)	2041, 2063
BspPI	GGATC	6 cut(s)	1074, 1579, 1962, 2056, 2069, 2213
BspT104I	TTCGAA	1 cut(s)	1055
BsrFI	RCCGGY	1 cut(s)	953
BsrI	ACTGG	3 cut(s)	426, 1533, 2362
BssAI	RCCGGY	1 cut(s)	953
BssECI	CCNNGG	5 cut(s)	270, 285, 1588, 1983, 2477
BssNAI	GTATAC	1 cut(s)	618
BssSI	CACGAG	3 cut(s)	159, 1231, 1423
BssT1I	CCWWGG	3 cut(s)	270, 1588, 1983
Bst1107I	GTATAC	1 cut(s)	618
Bst2BI	CACGAG	3 cut(s)	159, 1231, 1423
Bst2UI	CCWGG	4 cut(s)	1085, 2235, 2478, 2551
Bst4CI	ACNGT	8 cut(s)	245, 724, 854, 1205, 1732, 2291, 2435, 2525
Bst6I	CTCTTC	3 cut(s)	57, 124, 2324
BstBAI	YACGTR	1 cut(s)	779
BstBI	TTCGAA	1 cut(s)	1055
BstC8I	GCNNGC	4 cut(s)	1407, 1555, 1754, 1852
BstDEI	CTNAG	4 cut(s)	382, 1070, 1387, 1491
BstDSI	CCRYGG	2 cut(s)	270, 1588
BstF5I	GGATG	4 cut(s)	600, 662, 814, 1024
BstFNI	CGCG	1 cut(s)	174
BstH2I	RGCGCY	1 cut(s)	983
BstHHI	GCGC	1 cut(s)	982
BstMAI	GTCTC	6 cut(s)	51, 181, 1426, 2280, 2370, 2574
BstMCI	CGRYCG	1 cut(s)	805
BstMWI	GCNNNNNNNGC	8 cut(s)	20, 950, 986, 1001, 1559, 1781, 2017, 2402
BstNI	CCWGG	4 cut(s)	1085, 2235, 2478, 2551
BstNSI	RCATGY	5 cut(s)	1251, 1631, 1654, 1756, 2105
BstSCI	CCNGG	5 cut(s)	284, 1083, 2233, 2476, 2549
BstSFI	CTRYAG	1 cut(s)	2239
BstSLI	GKGCMC	4 cut(s)	166, 778, 1146, 2433
BstUI	CGCG	1 cut(s)	174
BstV1I	GCAGC	3 cut(s)	1574, 1768, 2293
BstV2I	GAAGAC	1 cut(s)	1454
BstX2I	RGATCY	5 cut(s)	1066, 1584, 1714, 1954, 2061
BstXI	CCANNNNNNTGG	1 cut(s)	277
BstYI	RGATCY	5 cut(s)	1066, 1584, 1714, 1954, 2061
BstZ17I	GTATAC	1 cut(s)	618
BsuI	GTATCC	3 cut(s)	1547, 1604, 2454
BsuRI	GGCC	5 cut(s)	14, 153, 2361, 2420, 2537
BtgI	CCRYGG	2 cut(s)	270, 1588
BtgZI	GCGATG	1 cut(s)	1887
BtrI	CACGTC	1 cut(s)	2074
BtsCI	GGATG	4 cut(s)	600, 662, 814, 1024
BtsIMutI	CAGTG	1 cut(s)	2440
Cac8I	GCNNGC	4 cut(s)	1407, 1555, 1754, 1852
CciI	TCATGA	1 cut(s)	2527
CfoI	GCGC	1 cut(s)	982
Cfr10I	RCCGGY	1 cut(s)	953
Cfr13I	GGNCC	2 cut(s)	151, 2419
CseI	GACGC	1 cut(s)	163
Csp6I	GTAC	5 cut(s)	241, 571, 799, 1670, 2141
CviQI	GTAC	5 cut(s)	241, 571, 799, 1670, 2141
DdeI	CTNAG	4 cut(s)	382, 1070, 1387, 1491
DrdI	GACNNNNNNGTC	2 cut(s)	1019, 1655
DseDI	GACNNNNNNGTC	2 cut(s)	1019, 1655
EaeI	YGGCCR	2 cut(s)	12, 2535
Eam1104I	CTCTTC	3 cut(s)	57, 124, 2324
EarI	CTCTTC	3 cut(s)	57, 124, 2324
EciI	GGCGGA	2 cut(s)	256, 2420
Eco130I	CCWWGG	3 cut(s)	270, 1588, 1983
Eco24I	GRGCYC	2 cut(s)	292, 2121
Eco32I	GATATC	1 cut(s)	689
Eco88I	CYCGRG	1 cut(s)	1318
EcoRI	GAATTC	1 cut(s)	823
EcoRII	CCWGG	4 cut(s)	1083, 2233, 2476, 2549
EcoRV	GATATC	1 cut(s)	689
EcoT14I	CCWWGG	3 cut(s)	270, 1588, 1983
EcoT22I	ATGCAT	3 cut(s)	991, 1249, 1758
EcoT38I	GRGCYC	2 cut(s)	292, 2121
ErhI	CCWWGG	3 cut(s)	270, 1588, 1983
Esp3I	CGTCTC	1 cut(s)	181
FaqI	GGGAC	1 cut(s)	1214
FauI	CCCGC	5 cut(s)	471, 657, 1055, 1658, 1973
FauNDI	CATATG	2 cut(s)	991, 2601
FblI	GTMKAC	2 cut(s)	617, 1647
Fnu4HI	GCNGC	4 cut(s)	192, 1563, 1782, 2307
FokI	GGATG	4 cut(s)	587, 669, 821, 1031
FriOI	GRGCYC	2 cut(s)	292, 2121
Fsp4HI	GCNGC	4 cut(s)	192, 1563, 1782, 2307
FspBI	CTAG	7 cut(s)	1043, 1254, 1559, 1673, 2081, 2114, 2559
GlaI	GCGC	1 cut(s)	981
GluI	GCNGC	4 cut(s)	192, 1563, 1782, 2307
GsaI	CCCAGC	1 cut(s)	974
GsuI	CTGGAG	2 cut(s)	2304, 2533
HaeII	RGCGCY	1 cut(s)	983
HaeIII	GGCC	5 cut(s)	14, 153, 2361, 2420, 2537
HapII	CCGG	3 cut(s)	285, 954, 1544
HgaI	GACGC	1 cut(s)	163
HhaI	GCGC	1 cut(s)	982
Hin6I	GCGC	1 cut(s)	980
HinP1I	GCGC	1 cut(s)	980
HincII	GTYRAC	1 cut(s)	1648
HindII	GTYRAC	1 cut(s)	1648
HindIII	AAGCTT	1 cut(s)	1002
HpaII	CCGG	3 cut(s)	285, 954, 1544
HphI	GGTGA	5 cut(s)	196, 1115, 2506, 2531, 2588
Hpy166II	GTNNAC	6 cut(s)	379, 571, 618, 776, 1648, 2294
Hpy8I	GTNNAC	6 cut(s)	379, 571, 618, 776, 1648, 2294
HpyCH4III	ACNGT	8 cut(s)	245, 724, 854, 1205, 1732, 2291, 2435, 2525
HpyCH4IV	ACGT	3 cut(s)	620, 778, 2073
HpyF10VI	GCNNNNNNNGC	8 cut(s)	20, 950, 986, 1001, 1559, 1781, 2017, 2402
HpyF3I	CTNAG	4 cut(s)	382, 1070, 1387, 1491
HpySE526I	ACGT	3 cut(s)	620, 778, 2073
HspAI	GCGC	1 cut(s)	980
LmnI	GCTCC	3 cut(s)	329, 334, 592
Lsp1109I	GCAGC	3 cut(s)	1574, 1768, 2293
LweI	GCATC	7 cut(s)	576, 609, 1207, 1369, 1488, 1998, 2014
MaeI	CTAG	7 cut(s)	1043, 1254, 1559, 1673, 2081, 2114, 2559
MaeII	ACGT	3 cut(s)	620, 778, 2073
MaeIII	GTNAC	5 cut(s)	98, 392, 1445, 2494, 2519
MfeI	CAATTG	1 cut(s)	1527
MflI	RGATCY	5 cut(s)	1066, 1584, 1714, 1954, 2061
MhlI	GDGCHC	7 cut(s)	166, 292, 778, 1146, 1938, 2121, 2433
MlsI	TGGCCA	2 cut(s)	14, 2537
MluNI	TGGCCA	2 cut(s)	14, 2537
MlyI	GAGTC	4 cut(s)	176, 1310, 2327, 2576
MmeI	TCCRAC	3 cut(s)	204, 706, 2225
Mox20I	TGGCCA	2 cut(s)	14, 2537
Mph1103I	ATGCAT	3 cut(s)	991, 1249, 1758
MroXI	GAANNNNTTC	1 cut(s)	678
MscI	TGGCCA	2 cut(s)	14, 2537
MslI	CAYNNNNRTG	6 cut(s)	275, 655, 990, 1248, 1349, 2492
Msp20I	TGGCCA	2 cut(s)	14, 2537
MspI	CCGG	3 cut(s)	285, 954, 1544
MspR9I	CCNGG	5 cut(s)	286, 1085, 2235, 2478, 2551
MunI	CAATTG	1 cut(s)	1527
MvaI	CCWGG	4 cut(s)	1085, 2235, 2478, 2551
MvnI	CGCG	1 cut(s)	174
MwoI	GCNNNNNNNGC	8 cut(s)	20, 950, 986, 1001, 1559, 1781, 2017, 2402
NciI	CCSGG	1 cut(s)	286
NcoI	CCATGG	2 cut(s)	270, 1588
NdeI	CATATG	2 cut(s)	991, 2601
NlaIV	GGNNCC	2 cut(s)	2041, 2063
NmeAIII	GCCGAG	1 cut(s)	2191
NmuCI	GTSAC	3 cut(s)	392, 2494, 2519
NsiI	ATGCAT	3 cut(s)	991, 1249, 1758
NspI	RCATGY	5 cut(s)	1251, 1631, 1654, 1756, 2105
NspV	TTCGAA	1 cut(s)	1055
PaeI	GCATGC	1 cut(s)	1756
PaeR7I	CTCGAG	1 cut(s)	1318
PagI	TCATGA	1 cut(s)	2527
PciI	ACATGT	2 cut(s)	1650, 2101
PdmI	GAANNNNTTC	1 cut(s)	678
PfeI	GAWTC	8 cut(s)	520, 679, 757, 961, 1702, 1887, 1975, 2230
PflFI	GACNNNGTC	1 cut(s)	1418
PfoI	TCCNGGA	1 cut(s)	2549
PkrI	GCNGC	4 cut(s)	193, 1564, 1783, 2308
Ple19I	CGATCG	1 cut(s)	805
PleI	GAGTC	4 cut(s)	176, 1310, 2326, 2576
PpsI	GAGTC	4 cut(s)	176, 1310, 2326, 2576
Ppu21I	YACGTR	1 cut(s)	779
PscI	ACATGT	2 cut(s)	1650, 2101
PshBI	ATTAAT	2 cut(s)	531, 2391
Psp6I	CCWGG	4 cut(s)	1083, 2233, 2476, 2549
PspFI	CCCAGC	1 cut(s)	970
PspGI	CCWGG	4 cut(s)	1083, 2233, 2476, 2549
PspN4I	GGNNCC	2 cut(s)	2041, 2063
PspPI	GGNCC	2 cut(s)	151, 2419
PspXI	VCTCGAGB	1 cut(s)	1318
PsuI	RGATCY	5 cut(s)	1066, 1584, 1714, 1954, 2061
PsyI	GACNNNGTC	1 cut(s)	1418
PvuI	CGATCG	1 cut(s)	805
RsaI	GTAC	5 cut(s)	242, 572, 800, 1671, 2142
RsaNI	GTAC	5 cut(s)	241, 571, 799, 1670, 2141
RseI	CAYNNNNRTG	6 cut(s)	275, 655, 990, 1248, 1349, 2492
SalI	GTCGAC	1 cut(s)	1646
SatI	GCNGC	4 cut(s)	192, 1563, 1782, 2307
Sau96I	GGNCC	2 cut(s)	151, 2419
ScaI	AGTACT	1 cut(s)	242
SchI	GAGTC	4 cut(s)	176, 1310, 2327, 2576
ScrFI	CCNGG	5 cut(s)	286, 1085, 2235, 2478, 2551
SduI	GDGCHC	7 cut(s)	166, 292, 778, 1146, 1938, 2121, 2433
SfaNI	GCATC	7 cut(s)	576, 609, 1207, 1369, 1488, 1998, 2014
SfcI	CTRYAG	1 cut(s)	2239
Sfr274I	CTCGAG	1 cut(s)	1318
SfuI	TTCGAA	1 cut(s)	1055
SlaI	CTCGAG	1 cut(s)	1318
SmiMI	CAYNNNNRTG	6 cut(s)	275, 655, 990, 1248, 1349, 2492
SmlI	CTYRAG	1 cut(s)	1318
SmoI	CTYRAG	1 cut(s)	1318
SpeI	ACTAGT	1 cut(s)	1672
SphI	GCATGC	1 cut(s)	1756
SspMI	CTAG	7 cut(s)	1043, 1254, 1559, 1673, 2081, 2114, 2559
StyD4I	CCNGG	5 cut(s)	284, 1083, 2233, 2476, 2549
StyI	CCWWGG	3 cut(s)	270, 1588, 1983
TaaI	ACNGT	8 cut(s)	245, 724, 854, 1205, 1732, 2291, 2435, 2525
TaiI	ACGT	3 cut(s)	623, 781, 2076
TatI	WGTACW	3 cut(s)	240, 570, 2140
TauI	GCSGC	1 cut(s)	194
TfiI	GAWTC	8 cut(s)	520, 679, 757, 961, 1702, 1887, 1975, 2230
TscAI	CASTG	1 cut(s)	2440
TseFI	GTSAC	3 cut(s)	392, 2494, 2519
TseI	GCWGC	3 cut(s)	1562, 1781, 2306
Tsp45I	GTSAC	3 cut(s)	392, 2494, 2519
TspGWI	ACGGA	2 cut(s)	316, 381
TspRI	CASTG	1 cut(s)	2440
Tth111I	GACNNNGTC	1 cut(s)	1418
VneI	GTGCAC	1 cut(s)	774
VspI	ATTAAT	2 cut(s)	531, 2391
XapI	RAATTY	4 cut(s)	145, 296, 730, 823
XbaI	TCTAGA	1 cut(s)	1042
XceI	RCATGY	5 cut(s)	1251, 1631, 1654, 1756, 2105
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2241
XhoI	CTCGAG	1 cut(s)	1318
XmiI	GTMKAC	2 cut(s)	617, 1647
XmnI	GAANNNNTTC	1 cut(s)	678
XspI	CTAG	7 cut(s)	1043, 1254, 1559, 1673, 2081, 2114, 2559
ZrmI	AGTACT	1 cut(s)	242
Zsp2I	ATGCAT	3 cut(s)	991, 1249, 1758

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.