Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0016045

Physical Location & Seq

Genomic Coordinates Forward (+)

8169682 .. 8172554

Gene Structure 2873 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G007710.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2637 bp

ATGTTGATGCTCTGCACAGCCTCACCAAACCAGTCATGGGTCTCTCCTTCTTCTTCTTACCATGACATAAAGCCAAAGCCTTTGAAGCCCTGCTTCATCTTCTTCAAACCCAATTCAACAACCTCTCTGTTATCTCTGAATTTCTCTAACTTTCGCGCCAAATTTCGTGTCTCGGCGACCACCCTCGCCGGAACCACTGACCGGAAAACTGCGGACAAGAATGTGAAGATTGGCGAACTCTGCGAATTGGGTAATCTGAAAGAGGCGGTGGAAATGGTGTGCGGGTCTGAAAGCTCCGATCTTGATTTGGTGACTTACTGCTCCGTGTTGGAGCTCTGTGCCGGAACTAAGTCTCTGCAAGATGGGAAGAGGGTCCGCTCTGTTCTTTGTGACCATGGAGTAGATGTTGATGGGTATGTGGGTGCAAAACTTGTGTTCATGTACGTGCATTGTGGGGATTTAAGTGAAGCGAGGCGGGTTTTCGATGAATTATCGAATGCGAAAGTGTTTCTATGGAACCTTTTGATCAATGAGTATGCGAAGATTCGTAATTTTAGAGAAGGTGTGTACTTATTTGGGAAGATGCAGGAGTTGGGTATCCAACCGAATTCGTATACGTTTTCTTGTATTTTGAAGTGTTTTGCTGCTCTTGGGTGTGTTAAGGAAGGTGAGTGGGTTCATGGGTATGTGTGGAAACTGGGTTTTAGCTCTGATAATACTGTGGTGAATTCTCTGATGGCTTTCTATTTCAAGAATAGAAGAGTTGAAAGTGCACGGAAGGTGTTTGATGAATTGCGTGACAGAGATGTGATATCGTGGAATTCGATGATTAGTGCGTATGTGTCGAATGGTGCTGCGGAGAAGGGAGTGGAGGTTTTCAGGCAGATGTTTTGTTCTGGGATTGATGTGGACTTGGCTACGATTATCAATGTTCTAATGGCGTGTTCGGACTGTGGAGATCTTGCATTGGGTAGGGTGGTTCATGCTTACGCGATAAAAGCTGGTTTCGACATGGATGTTATGTTGTGTAATAATGTTCTAGATATGTATTCAAAGTGTGGCGATTTGAGTGATGCAGTTCGAGTTTTTGAGAAGATGGGTCAAAGAAGTGTTGTGTCGTGGACTTCGATGATAGCAGGTTATGTTCGTGAAGGTCTTTCGGACAAGGCAATTGAGTTGTTTTGTGAAATGGAAGAGAATGGTGTTAGCCCAGATGGTTATACAATCACAAGCATACTTCATGCATGTGCTTGTAGTGGCTCGTTGGAAAAAGGCAAGGATACACACAAGTATATTAGGGAACATGGCATGGACTCGAGTTTGTTTGTCTGTAATTCGCTCATGGATATGTATGCAAAATGTGGAAGCATGGAAGACGCTGACTCAGTTTTTTCTCATATGCCTAGGAAGGACATTGTGTCATGGAATACCATGATCGGAGGTTACTCCAAAAACTGTCTTCCAAATGAAGCTCTAGAACTCTTTTCTGAGATGCAGCAGAAATTTAAACCTGACAGCATGACAATTGCTAGTGTCCTTCCCGCTTGTGCAAGCCTAGCAGCTCTAGACAGAGGTCAAGAGATCCATGGTTATTTACTGAGAAATGGATACTTCTTGGATCGATATGTTGCCAATGCACTTGTTGACATGTATGTCAAGTGCGGGGTACTAGTTCTTGCTCGGGTACTGTTTGATATGATTCCGGTGAAGGATCTGATCTCTTGGACTGTGATAATTGCTGGTTATGGGATGCATGGATTTGGAAGGGAAGCTATTGCTGCGTTTAATGAGATGAAAAAGGCTGGAATTGAACCGGACAGCATTTCCTTCATCTCTATACTTTATGCTTGCAGTCATTCTGGATTAGTTCAGGAAGGATGGAGATTCTTTGATATTATGAGAAATGATTACAAAATCGAGCCCATGTTGGAGCATTATGCTTGTATGGTAGATCTTCTTGCTCGTACTGGGAATCTAACCAAGGCATATAAATTTATTAACATGATGCCAATTGAGCCAGATGCCACAGTCTGGGGTTCCTTGCTCTGTGGTTGTAGGATTCACCATGATGTGAAACTAGCAGAGAAAGTTGCAGAGCATGTCTTTGAACTAGAGCCAGAAAACACCGGCTATTATATACTTCTAGCAAATATTTATGCAGAGGCAGAAAAGTGGGAAGAGGTGAAGAAGTTGAGGGAGAGGATTGGTCGGCGAGGCTTGAAAAAGAATCCGGGCTGTAGTTGGATAGAAATCAAGGGAAAAGTTAATATCTTTGTTGCGGGAGACACTTCACACCCAGAGGCCATGAAGATAGAGTCCCTAGTGAAGAAGTTTAGATTGAGGATGAAGGAAGATGGCTATAATCCTAAAATGCAGTACGCTTTGATTAATGCAGATGAGGTGGAAAAAGAGGTGGCTCTCTGTGGGCATAGTGAGAAGTTAGCCATAGCATTTGGGATACTGAACTTGCCACCCGGAAAAACCATACGGGTGACCAAGAATCTACGAGTCTGCGGTGACTGCCATGAGATGGCCAAGTTCATATCCAAGACATCTAGGAGGGAAATTGTTTTGAGAGATTCTAATCGTTTTCATCATATGAAGGATGGAAATTGTTCTTGTAGGGGCTTCTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003002 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0006139 GO:0006725 GO:0006807 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009451 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009965 GO:0009987 GO:0010016 GO:0010051 GO:0010087 GO:0010154 GO:0010305 GO:0010588 GO:0016070 GO:0016787 GO:0016788 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0046483 GO:0048316 GO:0048366 GO:0048367 GO:0048598 GO:0048608 GO:0048731 GO:0048825 GO:0048826 GO:0048827 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0090305 GO:0090698 GO:0097159 GO:0099402 GO:1900865 GO:1901360 GO:1901363 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

878

Amino Acids

98.52

Weight (kDa)

6.73

Isoelectric Point (pI)

34.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	169 - 215	9.6e-11	PPR repeat family
PPR_2	PF13041	268 - 308	6.1e-07	PPR repeat family
PPR	PF01535	271 - 301	1.7e-06	PPR repeat
PPR_2	PF13041	370 - 417	9e-15	PPR repeat family
PPR	PF01535	372 - 402	5.7e-10	PPR repeat
PPR_2	PF13041	470 - 517	3.8e-10	PPR repeat family
PPR	PF01535	473 - 501	1.7e-08	PPR repeat
PPR_2	PF13041	571 - 618	2e-09	PPR repeat family
PPR	PF01535	573 - 603	6.1e-06	PPR repeat
E_motif	PF20431	688 - 750	1.8e-20	E motif
DYW_deaminase	PF14432	786 - 878	2.1e-38	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016045)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G18750
fragaria_vesca	FvH4_3g36560 FvH4_3g36640
malus_domestica	MD10G1276800.v1.1
prunus_persica	Prupe.4G065400_v2.0.a1
pyrus_communis	pycom10g23110
rosa_chinensis	RchiOBHm_Chr5g0011421
rosa_laevigata	RLG00000031832
rosa_multiflora	Rmu_co8262965.1_g000001 Rmu_sc0009975.1_g000017
rosa_roxburghii	Rroxscaffold_1G00065030
rosa_rugosa	Rorug04G0456900
rosa_samantha	Rh5BG083800 Rh5CG097300
rosa_wichuraiana	Rw5G007710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1652
Acc36I	ACCTGC	1 cut(s)	1127
AccB7I	CCANNNNNTGG	2 cut(s)	2031, 2530
AccBSI	CCGCTC	1 cut(s)	378
AccI	GTMKAC	1 cut(s)	614
AccII	CGCG	2 cut(s)	156, 992
AclWI	GGATC	3 cut(s)	1576, 1626, 1719
AcoI	YGGCCR	1 cut(s)	2532
AcsI	RAATTY	7 cut(s)	139, 161, 607, 727, 820, 1502, 1991
AfaI	GTAC	6 cut(s)	443, 569, 1668, 1686, 1966, 2378
AfiI	CCNNNNNNNGG	4 cut(s)	37, 201, 2031, 2530
AflIII	ACRYGT	1 cut(s)	1647
AhdI	GACNNNNNGTC	1 cut(s)	1417
AhlI	ACTAGT	1 cut(s)	1669
AluBI	AGCT	7 cut(s)	294, 334, 708, 1001, 1472, 1562, 1772
AluI	AGCT	7 cut(s)	294, 334, 708, 1001, 1472, 1562, 1772
Alw21I	GWGCWC	2 cut(s)	336, 775
Alw26I	GTCTC	4 cut(s)	46, 175, 357, 2277
Alw44I	GTGCAC	1 cut(s)	771
AlwI	GGATC	3 cut(s)	1576, 1626, 1719
Ama87I	CYCGRG	2 cut(s)	1315, 1680
AoxI	GGCC	2 cut(s)	2301, 2532
ApaLI	GTGCAC	1 cut(s)	771
ApeKI	GCWGC	5 cut(s)	644, 854, 1495, 1559, 1778
ApoI	RAATTY	7 cut(s)	139, 161, 607, 727, 820, 1502, 1991
ArsI	GACNNNNNNTTYG	2 cut(s)	153, 185
AseI	ATTAAT	1 cut(s)	2388
Asp700I	GAANNNNTTC	1 cut(s)	2614
AspA2I	CCTAGG	1 cut(s)	1403
AspLEI	GCGC	1 cut(s)	158
AspS9I	GGNCC	1 cut(s)	373
AsuC2I	CCSGG	2 cut(s)	2232, 2475
AsuHPI	GGTGA	9 cut(s)	15, 322, 680, 736, 1717, 2054, 2194, 2503, 2528
AvaI	CYCGRG	2 cut(s)	1315, 1680
AvaII	GGWCC	1 cut(s)	373
AvrII	CCTAGG	1 cut(s)	1403
BaeGI	GKGCMC	1 cut(s)	775
BalI	TGGCCA	1 cut(s)	2534
BanII	GRGCYC	2 cut(s)	336, 1923
BbsI	GAAGAC	2 cut(s)	1380, 1451
Bbv12I	GWGCWC	2 cut(s)	336, 775
BbvI	GCAGC	5 cut(s)	631, 841, 1507, 1571, 1765
BccI	CCATC	9 cut(s)	356, 404, 730, 1090, 1208, 1872, 2348, 2524, 2600
BciVI	GTATCC	4 cut(s)	608, 1273, 1601, 2451
BclI	TGATCA	1 cut(s)	525
BcnI	CCSGG	2 cut(s)	2232, 2475
BcoDI	GTCTC	4 cut(s)	46, 175, 357, 2277
BcuI	ACTAGT	1 cut(s)	1669
BfmI	CTRYAG	1 cut(s)	2236
BfuAI	ACCTGC	1 cut(s)	1127
BfuI	GTATCC	4 cut(s)	608, 1273, 1601, 2451
BglII	AGATCT	2 cut(s)	958, 1951
BisI	GCNGC	5 cut(s)	645, 855, 1496, 1560, 1779
BlnI	CCTAGG	1 cut(s)	1403
BlsI	GCNGC	5 cut(s)	646, 856, 1497, 1561, 1780
Bme1390I	CCNGG	2 cut(s)	2232, 2475
Bme18I	GGWCC	1 cut(s)	373
BmeRI	GACNNNNNGTC	1 cut(s)	1417
BmeT110I	CYCGRG	2 cut(s)	1315, 1680
BmgT120I	GGNCC	1 cut(s)	373
BmiI	GGNNCC	4 cut(s)	193, 374, 518, 2038
BmrFI	CCNGG	2 cut(s)	2232, 2475
BmrI	ACTGGG	2 cut(s)	707, 1977
BmsI	GCATC	6 cut(s)	573, 1063, 1482, 1740, 1995, 2011
BmuI	ACTGGG	2 cut(s)	707, 1977
BoxI	GACNNNNGTC	1 cut(s)	1572
BpiI	GAAGAC	2 cut(s)	1380, 1451
BpuMI	CCSGG	2 cut(s)	2232, 2475
Bsa29I	ATCGAT	1 cut(s)	1621
BsaAI	YACGTR	1 cut(s)	445
BsaBI	GATNNNNATC	2 cut(s)	2249, 2583
BsaI	GGTCTC	1 cut(s)	46
BsaJI	CCNNGG	4 cut(s)	394, 1403, 1585, 1980
BsaWI	WCCGGW	3 cut(s)	201, 1702, 1813
BsaXI	ACNNNNNCTCC	4 cut(s)	390, 420, 858, 888
Bsc4I	CCNNNNNNNGG	4 cut(s)	37, 201, 2031, 2530
Bse118I	RCCGGY	1 cut(s)	2126
Bse1I	ACTGG	3 cut(s)	31, 702, 1972
Bse8I	GATNNNNATC	2 cut(s)	2249, 2583
BseCI	ATCGAT	1 cut(s)	1621
BseDI	CCNNGG	4 cut(s)	394, 1403, 1585, 1980
BseGI	GGATG	5 cut(s)	1021, 1755, 1883, 2349, 2611
BseJI	GATNNNNATC	2 cut(s)	2249, 2583
BseLI	CCNNNNNNNGG	4 cut(s)	37, 201, 2031, 2530
BseMII	CTCAG	3 cut(s)	1398, 1479, 1589
BseNI	ACTGG	3 cut(s)	31, 702, 1972
BseSI	GKGCMC	1 cut(s)	775
BseXI	GCAGC	5 cut(s)	631, 841, 1507, 1571, 1765
Bsh1236I	CGCG	2 cut(s)	156, 992
BshFI	GGCC	2 cut(s)	2303, 2534
BshVI	ATCGAT	1 cut(s)	1621
BsiHKAI	GWGCWC	2 cut(s)	336, 775
BsiHKCI	CYCGRG	2 cut(s)	1315, 1680
BsiSI	CCGG	8 cut(s)	189, 202, 342, 1703, 1814, 2127, 2231, 2475
BslFI	GGGAC	1 cut(s)	2302
BslI	CCNNNNNNNGG	4 cut(s)	37, 201, 2031, 2530
BsmAI	GTCTC	4 cut(s)	46, 175, 357, 2277
BsmFI	GGGAC	1 cut(s)	2302
BsmI	GAATGC	1 cut(s)	502
BsnI	GGCC	2 cut(s)	2303, 2534
Bso31I	GGTCTC	1 cut(s)	46
BsoBI	CYCGRG	2 cut(s)	1315, 1680
Bsp1286I	GDGCHC	3 cut(s)	336, 775, 1923
Bsp143I	GATC	9 cut(s)	298, 525, 958, 1434, 1581, 1618, 1711, 1716, 1951
Bsp19I	CCATGG	2 cut(s)	394, 1585
BspANI	GGCC	2 cut(s)	2303, 2534
BspCNI	CTCAG	3 cut(s)	1397, 1480, 1590
BspDI	ATCGAT	1 cut(s)	1621
BspFNI	CGCG	2 cut(s)	156, 992
BspLI	GGNNCC	4 cut(s)	193, 374, 518, 2038
BspMI	ACCTGC	1 cut(s)	1127
BspPI	GGATC	3 cut(s)	1576, 1626, 1719
BspTNI	GGTCTC	1 cut(s)	46
BsrBI	CCGCTC	1 cut(s)	378
BsrFI	RCCGGY	1 cut(s)	2126
BsrI	ACTGG	3 cut(s)	31, 702, 1972
BssAI	RCCGGY	1 cut(s)	2126
BssECI	CCNNGG	4 cut(s)	394, 1403, 1585, 1980
BssMI	GATC	9 cut(s)	298, 525, 958, 1434, 1581, 1618, 1711, 1716, 1951
BssNAI	GTATAC	1 cut(s)	615
BssT1I	CCWWGG	4 cut(s)	394, 1403, 1585, 1980
Bst1107I	GTATAC	1 cut(s)	615
Bst4CI	ACNGT	6 cut(s)	721, 953, 1457, 1689, 1729, 2029
Bst6I	CTCTTC	4 cut(s)	362, 754, 1188, 2172
BstBAI	YACGTR	1 cut(s)	445
BstC8I	GCNNGC	2 cut(s)	1552, 1849
BstDEI	CTNAG	4 cut(s)	348, 1384, 1488, 1598
BstDSI	CCRYGG	2 cut(s)	394, 1585
BstEII	GGTNACC	1 cut(s)	2491
BstF5I	GGATG	5 cut(s)	1021, 1755, 1883, 2349, 2611
BstFNI	CGCG	2 cut(s)	156, 992
BstHHI	GCGC	1 cut(s)	158
BstKTI	GATC	9 cut(s)	301, 528, 961, 1437, 1584, 1621, 1714, 1719, 1954
BstMAI	GTCTC	4 cut(s)	46, 175, 357, 2277
BstMBI	GATC	9 cut(s)	298, 525, 958, 1434, 1581, 1618, 1711, 1716, 1951
BstMWI	GCNNNNNNNGC	7 cut(s)	85, 240, 998, 1556, 1778, 2014, 2520
BstNSI	RCATGY	3 cut(s)	1248, 1651, 2102
BstPAI	GACNNNNGTC	1 cut(s)	1572
BstPI	GGTNACC	1 cut(s)	2491
BstSCI	CCNGG	2 cut(s)	2230, 2473
BstSFI	CTRYAG	1 cut(s)	2236
BstSLI	GKGCMC	1 cut(s)	775
BstUI	CGCG	2 cut(s)	156, 992
BstV1I	GCAGC	5 cut(s)	631, 841, 1507, 1571, 1765
BstV2I	GAAGAC	2 cut(s)	1380, 1451
BstX2I	RGATCY	4 cut(s)	958, 1581, 1711, 1951
BstYI	RGATCY	4 cut(s)	958, 1581, 1711, 1951
BstZ17I	GTATAC	1 cut(s)	615
Bsu15I	ATCGAT	1 cut(s)	1621
BsuI	GTATCC	4 cut(s)	608, 1273, 1601, 2451
BsuRI	GGCC	2 cut(s)	2303, 2534
BsuTUI	ATCGAT	1 cut(s)	1621
BtgI	CCRYGG	2 cut(s)	394, 1585
BtsCI	GGATG	5 cut(s)	1021, 1755, 1883, 2349, 2611
BtsIMutI	CAGTG	1 cut(s)	195
BveI	ACCTGC	1 cut(s)	1127
Cac8I	GCNNGC	2 cut(s)	1552, 1849
CfoI	GCGC	1 cut(s)	158
Cfr10I	RCCGGY	1 cut(s)	2126
Cfr13I	GGNCC	1 cut(s)	373
ClaI	ATCGAT	1 cut(s)	1621
CseI	GACGC	1 cut(s)	1385
Csp6I	GTAC	6 cut(s)	442, 568, 1667, 1685, 1965, 2377
CviQI	GTAC	6 cut(s)	442, 568, 1667, 1685, 1965, 2377
DdeI	CTNAG	4 cut(s)	348, 1384, 1488, 1598
DpnI	GATC	9 cut(s)	300, 527, 960, 1436, 1583, 1620, 1713, 1718, 1953
DpnII	GATC	9 cut(s)	298, 525, 958, 1434, 1581, 1618, 1711, 1716, 1951
DraI	TTTAAA	1 cut(s)	1507
DrdI	GACNNNNNNGTC	1 cut(s)	1652
DriI	GACNNNNNGTC	1 cut(s)	1417
DseDI	GACNNNNNNGTC	1 cut(s)	1652
EaeI	YGGCCR	1 cut(s)	2532
Eam1104I	CTCTTC	4 cut(s)	362, 754, 1188, 2172
Eam1105I	GACNNNNNGTC	1 cut(s)	1417
EarI	CTCTTC	4 cut(s)	362, 754, 1188, 2172
Ecl136II	GAGCTC	1 cut(s)	334
Eco130I	CCWWGG	4 cut(s)	394, 1403, 1585, 1980
Eco24I	GRGCYC	2 cut(s)	336, 1923
Eco31I	GGTCTC	1 cut(s)	46
Eco32I	GATATC	1 cut(s)	813
Eco47I	GGWCC	1 cut(s)	373
Eco53kI	GAGCTC	1 cut(s)	334
Eco88I	CYCGRG	2 cut(s)	1315, 1680
Eco91I	GGTNACC	1 cut(s)	2491
EcoICRI	GAGCTC	1 cut(s)	334
EcoO65I	GGTNACC	1 cut(s)	2491
EcoRI	GAATTC	3 cut(s)	607, 727, 820
EcoRV	GATATC	1 cut(s)	813
EcoT14I	CCWWGG	4 cut(s)	394, 1403, 1585, 1980
EcoT22I	ATGCAT	2 cut(s)	1246, 1755
EcoT38I	GRGCYC	2 cut(s)	336, 1923
ErhI	CCWWGG	4 cut(s)	394, 1403, 1585, 1980
FalI	AAGNNNNNCTT	2 cut(s)	77, 109
FaqI	GGGAC	1 cut(s)	2302
FauI	CCCGC	5 cut(s)	275, 468, 1549, 1655, 2272
FauNDI	CATATG	2 cut(s)	1398, 2598
FbaI	TGATCA	1 cut(s)	525
FblI	GTMKAC	1 cut(s)	614
Fnu4HI	GCNGC	5 cut(s)	645, 855, 1496, 1560, 1779
FokI	GGATG	5 cut(s)	1028, 1762, 1890, 2356, 2618
FriOI	GRGCYC	2 cut(s)	336, 1923
Fsp4HI	GCNGC	5 cut(s)	645, 855, 1496, 1560, 1779
GlaI	GCGC	1 cut(s)	157
GluI	GCNGC	5 cut(s)	645, 855, 1496, 1560, 1779
HaeIII	GGCC	2 cut(s)	2303, 2534
HapII	CCGG	8 cut(s)	189, 202, 342, 1703, 1814, 2127, 2231, 2475
HgaI	GACGC	1 cut(s)	1385
HhaI	GCGC	1 cut(s)	158
Hin6I	GCGC	1 cut(s)	156
HinP1I	GCGC	1 cut(s)	156
HincII	GTYRAC	1 cut(s)	1645
HindII	GTYRAC	1 cut(s)	1645
HpaII	CCGG	8 cut(s)	189, 202, 342, 1703, 1814, 2127, 2231, 2475
HphI	GGTGA	9 cut(s)	15, 322, 680, 736, 1717, 2054, 2194, 2503, 2528
Hpy166II	GTNNAC	6 cut(s)	568, 615, 773, 910, 1122, 1645
Hpy188III	TCNNGA	9 cut(s)	302, 751, 1040, 1148, 1475, 1565, 1577, 1860, 1871
Hpy8I	GTNNAC	6 cut(s)	568, 615, 773, 910, 1122, 1645
HpyCH4III	ACNGT	6 cut(s)	721, 953, 1457, 1689, 1729, 2029
HpyCH4IV	ACGT	2 cut(s)	444, 617
HpyF10VI	GCNNNNNNNGC	7 cut(s)	85, 240, 998, 1556, 1778, 2014, 2520
HpyF3I	CTNAG	4 cut(s)	348, 1384, 1488, 1598
HpySE526I	ACGT	2 cut(s)	444, 617
HspAI	GCGC	1 cut(s)	156
Ksp22I	TGATCA	1 cut(s)	525
Kzo9I	GATC	9 cut(s)	298, 525, 958, 1434, 1581, 1618, 1711, 1716, 1951
LmnI	GCTCC	4 cut(s)	299, 326, 331, 1930
Lsp1109I	GCAGC	5 cut(s)	631, 841, 1507, 1571, 1765
LweI	GCATC	6 cut(s)	573, 1063, 1482, 1740, 1995, 2011
MaeII	ACGT	2 cut(s)	444, 617
MaeIII	GTNAC	6 cut(s)	310, 389, 797, 1442, 2491, 2516
MalI	GATC	9 cut(s)	300, 527, 960, 1436, 1583, 1620, 1713, 1718, 1953
MbiI	CCGCTC	1 cut(s)	378
MboI	GATC	9 cut(s)	298, 525, 958, 1434, 1581, 1618, 1711, 1716, 1951
MfeI	CAATTG	3 cut(s)	1170, 1524, 2010
MflI	RGATCY	4 cut(s)	958, 1581, 1711, 1951
MhlI	GDGCHC	3 cut(s)	336, 775, 1923
MlsI	TGGCCA	1 cut(s)	2534
MluNI	TGGCCA	1 cut(s)	2534
MlyI	GAGTC	4 cut(s)	1307, 1376, 2324, 2517
MmeI	TCCRAC	5 cut(s)	309, 625, 1245, 1908, 2222
Mox20I	TGGCCA	1 cut(s)	2534
Mph1103I	ATGCAT	2 cut(s)	1246, 1755
MroXI	GAANNNNTTC	1 cut(s)	2614
MscI	TGGCCA	1 cut(s)	2534
MseI	TTAA	7 cut(s)	461, 660, 1506, 1785, 1998, 2265, 2388
MslI	CAYNNNNRTG	6 cut(s)	443, 684, 1245, 1346, 2067, 2489
Msp20I	TGGCCA	1 cut(s)	2534
MspI	CCGG	8 cut(s)	189, 202, 342, 1703, 1814, 2127, 2231, 2475
MspR9I	CCNGG	2 cut(s)	2232, 2475
MunI	CAATTG	3 cut(s)	1170, 1524, 2010
Mva1269I	GAATGC	1 cut(s)	502
MvnI	CGCG	2 cut(s)	156, 992
MwoI	GCNNNNNNNGC	7 cut(s)	85, 240, 998, 1556, 1778, 2014, 2520
NciI	CCSGG	2 cut(s)	2232, 2475
NcoI	CCATGG	2 cut(s)	394, 1585
NdeI	CATATG	2 cut(s)	1398, 2598
NdeII	GATC	9 cut(s)	298, 525, 958, 1434, 1581, 1618, 1711, 1716, 1951
NlaIV	GGNNCC	4 cut(s)	193, 374, 518, 2038
NmeAIII	GCCGAG	1 cut(s)	152
NmuCI	GTSAC	5 cut(s)	310, 389, 797, 2491, 2516
NsiI	ATGCAT	2 cut(s)	1246, 1755
NspI	RCATGY	3 cut(s)	1248, 1651, 2102
PaeR7I	CTCGAG	1 cut(s)	1315
PciI	ACATGT	1 cut(s)	1647
PcsI	WCGNNNNNNNCGW	2 cut(s)	821, 1124
PctI	GAATGC	1 cut(s)	502
PdmI	GAANNNNTTC	1 cut(s)	2614
PfeI	GAWTC	8 cut(s)	544, 1699, 1884, 1972, 2059, 2227, 2500, 2579
PflMI	CCANNNNNTGG	2 cut(s)	2031, 2530
PkrI	GCNGC	5 cut(s)	646, 856, 1497, 1561, 1780
PleI	GAGTC	4 cut(s)	1307, 1376, 2323, 2516
PpsI	GAGTC	4 cut(s)	1307, 1376, 2323, 2516
Ppu21I	YACGTR	1 cut(s)	445
PscI	ACATGT	1 cut(s)	1647
PshAI	GACNNNNGTC	1 cut(s)	1572
PshBI	ATTAAT	1 cut(s)	2388
Psp124BI	GAGCTC	1 cut(s)	336
PspEI	GGTNACC	1 cut(s)	2491
PspN4I	GGNNCC	4 cut(s)	193, 374, 518, 2038
PspPI	GGNCC	1 cut(s)	373
PspXI	VCTCGAGB	1 cut(s)	1315
PsuI	RGATCY	4 cut(s)	958, 1581, 1711, 1951
RsaI	GTAC	6 cut(s)	443, 569, 1668, 1686, 1966, 2378
RsaNI	GTAC	6 cut(s)	442, 568, 1667, 1685, 1965, 2377
RseI	CAYNNNNRTG	6 cut(s)	443, 684, 1245, 1346, 2067, 2489
SacI	GAGCTC	1 cut(s)	336
SaqAI	TTAA	7 cut(s)	461, 660, 1506, 1785, 1998, 2265, 2388
SatI	GCNGC	5 cut(s)	645, 855, 1496, 1560, 1779
Sau3AI	GATC	9 cut(s)	298, 525, 958, 1434, 1581, 1618, 1711, 1716, 1951
Sau96I	GGNCC	1 cut(s)	373
SchI	GAGTC	4 cut(s)	1307, 1376, 2324, 2517
ScrFI	CCNGG	2 cut(s)	2232, 2475
SduI	GDGCHC	3 cut(s)	336, 775, 1923
SfaNI	GCATC	6 cut(s)	573, 1063, 1482, 1740, 1995, 2011
SfcI	CTRYAG	1 cut(s)	2236
Sfr274I	CTCGAG	1 cut(s)	1315
SinI	GGWCC	1 cut(s)	373
SlaI	CTCGAG	1 cut(s)	1315
SmiMI	CAYNNNNRTG	6 cut(s)	443, 684, 1245, 1346, 2067, 2489
SmlI	CTYRAG	1 cut(s)	1315
SmoI	CTYRAG	1 cut(s)	1315
SpeI	ACTAGT	1 cut(s)	1669
SspI	AATATT	1 cut(s)	2152
SstI	GAGCTC	1 cut(s)	336
StyD4I	CCNGG	2 cut(s)	2230, 2473
StyI	CCWWGG	4 cut(s)	394, 1403, 1585, 1980
TaaI	ACNGT	6 cut(s)	721, 953, 1457, 1689, 1729, 2029
TaiI	ACGT	2 cut(s)	447, 620
TatI	WGTACW	1 cut(s)	567
TfiI	GAWTC	8 cut(s)	544, 1699, 1884, 1972, 2059, 2227, 2500, 2579
Tru1I	TTAA	7 cut(s)	461, 660, 1506, 1785, 1998, 2265, 2388
Tru9I	TTAA	7 cut(s)	461, 660, 1506, 1785, 1998, 2265, 2388
TscAI	CASTG	1 cut(s)	202
TseFI	GTSAC	5 cut(s)	310, 389, 797, 2491, 2516
TseI	GCWGC	5 cut(s)	644, 854, 1495, 1559, 1778
Tsp45I	GTSAC	5 cut(s)	310, 389, 797, 2491, 2516
TspGWI	ACGGA	2 cut(s)	313, 790
TspRI	CASTG	1 cut(s)	202
Van91I	CCANNNNNTGG	2 cut(s)	2031, 2530
VneI	GTGCAC	1 cut(s)	771
VpaK11BI	GGWCC	1 cut(s)	373
VspI	ATTAAT	1 cut(s)	2388
XapI	RAATTY	7 cut(s)	139, 161, 607, 727, 820, 1502, 1991
XbaI	TCTAGA	3 cut(s)	1039, 1474, 1564
XceI	RCATGY	3 cut(s)	1248, 1651, 2102
XcmI	CCANNNNNNNNNTGG	1 cut(s)	33
XhoI	CTCGAG	1 cut(s)	1315
XmaJI	CCTAGG	1 cut(s)	1403
XmiI	GTMKAC	1 cut(s)	614
XmnI	GAANNNNTTC	1 cut(s)	2614
Zsp2I	ATGCAT	2 cut(s)	1246, 1755

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.