MD10G1282600.v1.1

Conserved hypothetical protein (Lin0512_fam)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0007945
Physical Location & Seq
Reverse (-)
37320872 .. 37323466
2595 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1282600.v1.1.491

Sequence Viewer

Length: 591 bp
ATGACGGCCTGCTTCTTTCTAAATCTGTCACTCTCTTCTTCTTCATCTACTGCGTTTGCATCTCACAGAAACTACCAATCACCAGCTTCCTTCCCTCCACTTCTCACTCTAATCCCAACCCAATTCTCCCATCTTCGGACCCCTCGATCCTCTCGCCAAATCAGAGCCGCATCAGCCGCCATGGAAGCCACCCAGCAGAGCGTGACCACCGCATCAGCGTCGCCCATGAAGCTCTTGTTCGTGGAGATGGGCGTCGGCTACGACCAACACGGGCAGGACGTTACGTCTGCGGCAATGCGGGCCTGCAGAGATGCCATCACTTCCAATTCAATCCCTGCGTTTCGAAGAGGGTCAATTCCTGGTGTGACATTTGAGCAGATGAAGCTAGAGATCAAGCTGGGAGTGCCTCGTGTACTCCAACCATCGCTGGATGTCGAGAAGGTCAAATCAGTCTTTCCTTATGGAGAAATTGTGAAAGTTGAAGTTGTGGATGGTGGACTGATATGCTCGAGTGGCGTGTTGGTGGAAGAAATGGGAGATAAGACTGACGACTGTTACATAGTGAATGCAGCAGTTTACATTGGTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lin0512_fam

Protein Analysis

197

Amino Acids

21.16

Weight (kDa)

6.82

Isoelectric Point (pI)

43.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lin0512_fam PF09585 79 - 193 7e-38 Conserved hypothetical protein (Lin0512_fam)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 168, 177, 210, 290, 298
AclWI GGATC 1 cut(s) 141
AcyI GRCGYC 1 cut(s) 252
AfaI GTAC 1 cut(s) 414
AfiI CCNNNNNNNGG 1 cut(s) 135
AgsI TTSAA 2 cut(s) 330, 482
AhdI GACNNNNNGTC 1 cut(s) 283
AjnI CCWGG 1 cut(s) 358
AluBI AGCT 4 cut(s) 86, 232, 385, 397
AluI AGCT 4 cut(s) 86, 232, 385, 397
AlwI GGATC 1 cut(s) 141
Ama87I CYCGRG 1 cut(s) 508
AoxI GGCC 2 cut(s) 6, 300
ApeKI GCWGC 1 cut(s) 569
AspS9I GGNCC 2 cut(s) 138, 300
AsuHPI GGTGA 1 cut(s) 72
AsuII TTCGAA 1 cut(s) 343
AvaI CYCGRG 1 cut(s) 508
AvaII GGWCC 1 cut(s) 138
BauI CACGAG 1 cut(s) 408
BbvI GCAGC 1 cut(s) 581
BccI CCATC 5 cut(s) 138, 241, 323, 430, 485
BceAI ACGGC 1 cut(s) 21
BcgI CGANNNNNNTGC 2 cut(s) 201, 235
BciT130I CCWGG 1 cut(s) 360
BfaI CTAG 2 cut(s) 386, 589
BfmI CTRYAG 1 cut(s) 304
BisI GCNGC 4 cut(s) 168, 177, 291, 570
BlsI GCNGC 4 cut(s) 169, 178, 292, 571
Bme1390I CCNGG 1 cut(s) 360
Bme18I GGWCC 1 cut(s) 138
BmeRI GACNNNNNGTC 1 cut(s) 283
BmeT110I CYCGRG 1 cut(s) 508
BmgT120I GGNCC 2 cut(s) 138, 300
BmiI GGNNCC 1 cut(s) 140
BmrFI CCNGG 1 cut(s) 360
BmsI GCATC 4 cut(s) 68, 179, 221, 301
Bpu14I TTCGAA 1 cut(s) 343
BsaHI GRCGYC 1 cut(s) 252
BsaJI CCNNGG 1 cut(s) 180
BsaXI ACNNNNNCTCC 2 cut(s) 456, 486
Bsc4I CCNNNNNNNGG 1 cut(s) 135
Bse3DI GCAATG 1 cut(s) 300
BseBI CCWGG 1 cut(s) 360
BseDI CCNNGG 1 cut(s) 180
BseGI GGATG 2 cut(s) 436, 496
BseLI CCNNNNNNNGG 1 cut(s) 135
BseMI GCAATG 1 cut(s) 300
BseXI GCAGC 1 cut(s) 581
BseYI CCCAGC 2 cut(s) 192, 397
BshFI GGCC 2 cut(s) 8, 302
BsiHKCI CYCGRG 1 cut(s) 508
BslI CCNNNNNNNGG 1 cut(s) 135
BsmI GAATGC 1 cut(s) 571
BsnI GGCC 2 cut(s) 8, 302
BsoBI CYCGRG 1 cut(s) 508
Bsp119I TTCGAA 1 cut(s) 343
Bsp143I GATC 2 cut(s) 146, 390
Bsp19I CCATGG 1 cut(s) 180
BspACI CCGC 5 cut(s) 168, 177, 210, 290, 298
BspANI GGCC 2 cut(s) 8, 302
BspLI GGNNCC 1 cut(s) 140
BspMAI CTGCAG 1 cut(s) 308
BspPI GGATC 1 cut(s) 141
BspT104I TTCGAA 1 cut(s) 343
BsrDI GCAATG 1 cut(s) 300
BssECI CCNNGG 1 cut(s) 180
BssMI GATC 2 cut(s) 146, 390
BssNI GRCGYC 1 cut(s) 252
BssSI CACGAG 1 cut(s) 408
BssT1I CCWWGG 1 cut(s) 180
Bst2BI CACGAG 1 cut(s) 408
Bst2UI CCWGG 1 cut(s) 360
Bst4CI ACNGT 1 cut(s) 554
Bst6I CTCTTC 2 cut(s) 40, 340
BstACI GRCGYC 1 cut(s) 252
BstBI TTCGAA 1 cut(s) 343
BstC8I GCNNGC 3 cut(s) 10, 300, 304
BstDSI CCRYGG 1 cut(s) 180
BstF5I GGATG 2 cut(s) 436, 496
BstKTI GATC 2 cut(s) 149, 393
BstMBI GATC 2 cut(s) 146, 390
BstMWI GCNNNNNNNGC 8 cut(s) 173, 176, 185, 229, 299, 382, 403, 513
BstNI CCWGG 1 cut(s) 360
BstSCI CCNGG 1 cut(s) 358
BstSFI CTRYAG 1 cut(s) 304
BstV1I GCAGC 1 cut(s) 581
BsuRI GGCC 2 cut(s) 8, 302
BtgI CCRYGG 1 cut(s) 180
BtgZI GCGATG 1 cut(s) 408
BtsCI GGATG 2 cut(s) 436, 496
Cac8I GCNNGC 3 cut(s) 10, 300, 304
Cfr13I GGNCC 2 cut(s) 138, 300
CseI GACGC 2 cut(s) 207, 241
Csp6I GTAC 1 cut(s) 413
CviAII CATG 2 cut(s) 181, 226
CviQI GTAC 1 cut(s) 413
DpnI GATC 2 cut(s) 148, 392
DpnII GATC 2 cut(s) 146, 390
DriI GACNNNNNGTC 1 cut(s) 283
Eam1104I CTCTTC 2 cut(s) 40, 340
Eam1105I GACNNNNNGTC 1 cut(s) 283
EarI CTCTTC 2 cut(s) 40, 340
Eco130I CCWWGG 1 cut(s) 180
Eco47I GGWCC 1 cut(s) 138
Eco88I CYCGRG 1 cut(s) 508
EcoRII CCWGG 1 cut(s) 358
EcoT14I CCWWGG 1 cut(s) 180
ErhI CCWWGG 1 cut(s) 180
FaeI CATG 2 cut(s) 184, 229
FaiI YATR 5 cut(s) 182, 227, 462, 505, 560
FatI CATG 2 cut(s) 180, 225
FauI CCCGC 1 cut(s) 291
Fnu4HI GCNGC 4 cut(s) 168, 177, 291, 570
FokI GGATG 2 cut(s) 443, 503
Fsp4HI GCNGC 4 cut(s) 168, 177, 291, 570
FspBI CTAG 2 cut(s) 386, 589
GluI GCNGC 4 cut(s) 168, 177, 291, 570
GsaI CCCAGC 2 cut(s) 196, 401
HaeIII GGCC 2 cut(s) 8, 302
HgaI GACGC 2 cut(s) 207, 241
Hin1I GRCGYC 1 cut(s) 252
Hin1II CATG 2 cut(s) 184, 229
HphI GGTGA 1 cut(s) 72
Hpy166II GTNNAC 3 cut(s) 413, 497, 577
Hpy188I TCNGA 2 cut(s) 138, 164
Hpy188III TCNNGA 1 cut(s) 436
Hpy8I GTNNAC 3 cut(s) 413, 497, 577
Hpy99I CGWCG 2 cut(s) 223, 257
HpyAV CCTTC 2 cut(s) 100, 433
HpyCH4III ACNGT 1 cut(s) 554
HpyCH4IV ACGT 2 cut(s) 279, 284
HpyCH4V TGCA 3 cut(s) 59, 306, 569
HpyF10VI GCNNNNNNNGC 8 cut(s) 173, 176, 185, 229, 299, 382, 403, 513
HpySE526I ACGT 2 cut(s) 279, 284
Hsp92I GRCGYC 1 cut(s) 252
Hsp92II CATG 2 cut(s) 184, 229
Kzo9I GATC 2 cut(s) 146, 390
Lsp1109I GCAGC 1 cut(s) 581
LweI GCATC 4 cut(s) 68, 179, 221, 301
MaeI CTAG 2 cut(s) 386, 589
MaeII ACGT 2 cut(s) 279, 284
MaeIII GTNAC 6 cut(s) 27, 202, 280, 364, 554, 584
MalI GATC 2 cut(s) 148, 392
MboI GATC 2 cut(s) 146, 390
MboII GAAGA 6 cut(s) 27, 30, 33, 125, 357, 539
MluCI AATT 4 cut(s) 122, 325, 354, 468
MmeI TCCRAC 1 cut(s) 442
MnlI CCTC 5 cut(s) 105, 153, 160, 341, 417
MspR9I CCNGG 1 cut(s) 360
Mva1269I GAATGC 1 cut(s) 571
MvaI CCWGG 1 cut(s) 360
MwoI GCNNNNNNNGC 8 cut(s) 173, 176, 185, 229, 299, 382, 403, 513
NcoI CCATGG 1 cut(s) 180
NdeII GATC 2 cut(s) 146, 390
NlaIII CATG 2 cut(s) 184, 229
NlaIV GGNNCC 1 cut(s) 140
NmuCI GTSAC 3 cut(s) 27, 202, 364
NspV TTCGAA 1 cut(s) 343
PaeR7I CTCGAG 1 cut(s) 508
PcsI WCGNNNNNNNCGW 2 cut(s) 142, 276
PctI GAATGC 1 cut(s) 571
PkrI GCNGC 4 cut(s) 169, 178, 292, 571
Psp6I CCWGG 1 cut(s) 358
PspFI CCCAGC 2 cut(s) 192, 397
PspGI CCWGG 1 cut(s) 358
PspN4I GGNNCC 1 cut(s) 140
PspPI GGNCC 2 cut(s) 138, 300
PspXI VCTCGAGB 1 cut(s) 508
PstI CTGCAG 1 cut(s) 308
RsaI GTAC 1 cut(s) 414
RsaNI GTAC 1 cut(s) 413
SatI GCNGC 4 cut(s) 168, 177, 291, 570
Sau3AI GATC 2 cut(s) 146, 390
Sau96I GGNCC 2 cut(s) 138, 300
ScrFI CCNGG 1 cut(s) 360
SetI ASST 7 cut(s) 88, 234, 282, 287, 387, 399, 444
SfaNI GCATC 4 cut(s) 68, 179, 221, 301
SfcI CTRYAG 1 cut(s) 304
Sfr274I CTCGAG 1 cut(s) 508
SfuI TTCGAA 1 cut(s) 343
SinI GGWCC 1 cut(s) 138
SlaI CTCGAG 1 cut(s) 508
SmlI CTYRAG 1 cut(s) 508
SmoI CTYRAG 1 cut(s) 508
Sse9I AATT 4 cut(s) 122, 325, 354, 468
SsiI CCGC 5 cut(s) 168, 177, 210, 290, 298
SspMI CTAG 2 cut(s) 386, 589
StyD4I CCNGG 1 cut(s) 358
StyI CCWWGG 1 cut(s) 180
TaaI ACNGT 1 cut(s) 554
TaiI ACGT 2 cut(s) 282, 287
TaqI TCGA 4 cut(s) 145, 343, 435, 509
TasI AATT 4 cut(s) 122, 325, 354, 468
TatI WGTACW 1 cut(s) 412
TauI GCSGC 3 cut(s) 170, 179, 293
TseFI GTSAC 3 cut(s) 27, 202, 364
TseI GCWGC 1 cut(s) 569
Tsp45I GTSAC 3 cut(s) 27, 202, 364
TspDTI ATGAA 3 cut(s) 33, 242, 395
VpaK11BI GGWCC 1 cut(s) 138
XhoI CTCGAG 1 cut(s) 508
XspI CTAG 2 cut(s) 386, 589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.