MD10G1295500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0005442
Physical Location & Seq
Forward (+)
38383918 .. 38386247
2330 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1295500.v1.1.491

Sequence Viewer

Length: 408 bp
ATGATACCGCATCGATGGGGGTCTCCGTGCGGCAACCAATGCACGCACAAGTACGCAGCGCTTATGCAGATTCCATGGAGAGTATTTTGCAAAAAAGGGTGCGATGCGGATGGAGAAACTTGGGAAGAATGCTTGGAGGAGTGCAATGAGATATGCTATAAGGATCCTGTCTTAAAGGACCAACAATGGAGTGCTTGCATTGACCGTTCTCCCGGAGTTGACAGGTACTCAGAGGAGTGTTTTCATGCTTGCGTATCTGGTTGCGGTTTCAAGTTTGATAAAACTCCAGACATAGCTGATAAAGTTCGTCCAAACAGGCCGGCTAAGCCTCCCCCTGTTGAAAAGCCCACCGCACCACCTGCTGAATCCACGGCAACAGCCGAAGACATACTTGGCACTTCTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

136

Amino Acids

15.01

Weight (kDa)

5.18

Isoelectric Point (pI)

62.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 367
Acc36I ACCTGC 1 cut(s) 367
AciI CCGC 5 cut(s) 8, 30, 107, 264, 351
AclWI GGATC 2 cut(s) 158, 171
AfaI GTAC 2 cut(s) 53, 227
AfeI AGCGCT 1 cut(s) 60
AgsI TTSAA 2 cut(s) 271, 341
AjuI GAANNNNNNNTTGG 2 cut(s) 375, 407
AluBI AGCT 1 cut(s) 296
AluI AGCT 1 cut(s) 296
Alw26I GTCTC 1 cut(s) 27
AlwI GGATC 2 cut(s) 158, 171
Aor51HI AGCGCT 1 cut(s) 60
AoxI GGCC 1 cut(s) 317
ApeKI GCWGC 1 cut(s) 56
AspLEI GCGC 1 cut(s) 61
AspS9I GGNCC 1 cut(s) 178
AsuC2I CCSGG 1 cut(s) 213
AvaII GGWCC 1 cut(s) 178
BamHI GGATCC 1 cut(s) 163
BbsI GAAGAC 1 cut(s) 390
BbvI GCAGC 1 cut(s) 68
BccI CCATC 2 cut(s) 9, 104
BceAI ACGGC 1 cut(s) 387
BcnI CCSGG 1 cut(s) 213
BcoDI GTCTC 1 cut(s) 27
BfoI RGCGCY 1 cut(s) 62
BfuAI ACCTGC 1 cut(s) 367
BisI GCNGC 2 cut(s) 31, 57
BlpI GCTNAGC 1 cut(s) 324
BlsI GCNGC 2 cut(s) 32, 58
Bme1390I CCNGG 1 cut(s) 213
Bme18I GGWCC 1 cut(s) 178
BmgT120I GGNCC 1 cut(s) 178
BmiI GGNNCC 1 cut(s) 165
BmrFI CCNGG 1 cut(s) 213
BmsI GCATC 2 cut(s) 19, 94
BpiI GAAGAC 1 cut(s) 390
BpmI CTGGAG 1 cut(s) 270
Bpu1102I GCTNAGC 1 cut(s) 324
BpuMI CCSGG 1 cut(s) 213
Bsa29I ATCGAT 1 cut(s) 13
BsaI GGTCTC 1 cut(s) 27
BsaJI CCNNGG 2 cut(s) 74, 369
Bse118I RCCGGY 1 cut(s) 319
Bse3DI GCAATG 1 cut(s) 151
BseCI ATCGAT 1 cut(s) 13
BseDI CCNNGG 2 cut(s) 74, 369
BseGI GGATG 1 cut(s) 115
BseMI GCAATG 1 cut(s) 151
BseMII CTCAG 1 cut(s) 243
BseRI GAGGAG 2 cut(s) 152, 248
BseXI GCAGC 1 cut(s) 68
BshFI GGCC 1 cut(s) 319
BshVI ATCGAT 1 cut(s) 13
BsiSI CCGG 2 cut(s) 213, 320
BsmAI GTCTC 1 cut(s) 27
BsmI GAATGC 1 cut(s) 134
BsnI GGCC 1 cut(s) 319
Bso31I GGTCTC 1 cut(s) 27
Bsp143I GATC 1 cut(s) 163
Bsp1720I GCTNAGC 1 cut(s) 324
Bsp19I CCATGG 1 cut(s) 74
BspACI CCGC 5 cut(s) 8, 30, 107, 264, 351
BspANI GGCC 1 cut(s) 319
BspCNI CTCAG 1 cut(s) 242
BspDI ATCGAT 1 cut(s) 13
BspLI GGNNCC 1 cut(s) 165
BspMI ACCTGC 1 cut(s) 367
BspPI GGATC 2 cut(s) 158, 171
BspTNI GGTCTC 1 cut(s) 27
BsrDI GCAATG 1 cut(s) 151
BsrFI RCCGGY 1 cut(s) 319
BssAI RCCGGY 1 cut(s) 319
BssECI CCNNGG 2 cut(s) 74, 369
BssMI GATC 1 cut(s) 163
BssT1I CCWWGG 1 cut(s) 74
Bst4CI ACNGT 1 cut(s) 206
BstAPI GCANNNNNTGC 2 cut(s) 39, 359
BstC8I GCNNGC 4 cut(s) 44, 196, 250, 321
BstDEI CTNAG 2 cut(s) 229, 324
BstDSI CCRYGG 2 cut(s) 74, 369
BstF5I GGATG 1 cut(s) 115
BstH2I RGCGCY 1 cut(s) 62
BstHHI GCGC 1 cut(s) 61
BstKTI GATC 1 cut(s) 166
BstMAI GTCTC 1 cut(s) 27
BstMBI GATC 1 cut(s) 163
BstMWI GCNNNNNNNGC 3 cut(s) 39, 325, 359
BstSCI CCNGG 1 cut(s) 211
BstV1I GCAGC 1 cut(s) 68
BstV2I GAAGAC 1 cut(s) 390
BstX2I RGATCY 1 cut(s) 163
BstYI RGATCY 1 cut(s) 163
Bsu15I ATCGAT 1 cut(s) 13
BsuRI GGCC 1 cut(s) 319
BsuTUI ATCGAT 1 cut(s) 13
BtgI CCRYGG 2 cut(s) 74, 369
BtgZI GCGATG 1 cut(s) 117
BtsCI GGATG 1 cut(s) 115
BveI ACCTGC 1 cut(s) 367
Cac8I GCNNGC 4 cut(s) 44, 196, 250, 321
CfoI GCGC 1 cut(s) 61
Cfr10I RCCGGY 1 cut(s) 319
Cfr13I GGNCC 1 cut(s) 178
ClaI ATCGAT 1 cut(s) 13
Csp6I GTAC 2 cut(s) 52, 226
CviAII CATG 2 cut(s) 75, 245
CviJI RGCY 6 cut(s) 296, 319, 323, 328, 346, 380
CviKI_1 RGCY 6 cut(s) 296, 319, 323, 328, 346, 380
CviQI GTAC 2 cut(s) 52, 226
DdeI CTNAG 2 cut(s) 229, 324
DpnI GATC 1 cut(s) 165
DpnII GATC 1 cut(s) 163
Eco130I CCWWGG 1 cut(s) 74
Eco31I GGTCTC 1 cut(s) 27
Eco47I GGWCC 1 cut(s) 178
Eco47III AGCGCT 1 cut(s) 60
EcoT14I CCWWGG 1 cut(s) 74
ErhI CCWWGG 1 cut(s) 74
FaeI CATG 2 cut(s) 78, 248
FaiI YATR 8 cut(s) 65, 76, 154, 159, 246, 293, 389, 406
FalI AAGNNNNNCTT 2 cut(s) 375, 407
FatI CATG 2 cut(s) 74, 244
Fnu4HI GCNGC 2 cut(s) 31, 57
FokI GGATG 1 cut(s) 122
Fsp4HI GCNGC 2 cut(s) 31, 57
GlaI GCGC 1 cut(s) 60
GluI GCNGC 2 cut(s) 31, 57
GsuI CTGGAG 1 cut(s) 270
HaeII RGCGCY 1 cut(s) 62
HaeIII GGCC 1 cut(s) 319
HapII CCGG 2 cut(s) 213, 320
HhaI GCGC 1 cut(s) 61
Hin1II CATG 2 cut(s) 78, 248
Hin6I GCGC 1 cut(s) 59
HinP1I GCGC 1 cut(s) 59
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HinfI GANTC 2 cut(s) 70, 365
HpaII CCGG 2 cut(s) 213, 320
Hpy166II GTNNAC 1 cut(s) 220
Hpy188I TCNGA 1 cut(s) 232
Hpy188III TCNNGA 1 cut(s) 287
Hpy8I GTNNAC 1 cut(s) 220
HpyCH4III ACNGT 1 cut(s) 206
HpyCH4V TGCA 6 cut(s) 42, 67, 90, 144, 198, 404
HpyF10VI GCNNNNNNNGC 3 cut(s) 39, 325, 359
HpyF3I CTNAG 2 cut(s) 229, 324
Hsp92II CATG 2 cut(s) 78, 248
HspAI GCGC 1 cut(s) 59
KroI GCCGGC 1 cut(s) 319
KroNI GCCGGC 1 cut(s) 321
Kzo9I GATC 1 cut(s) 163
LpnPI CCDG 9 cut(s) 180, 208, 226, 243, 300, 301, 333, 348, 372
Lsp1109I GCAGC 1 cut(s) 68
LweI GCATC 2 cut(s) 19, 94
MalI GATC 1 cut(s) 165
MboI GATC 1 cut(s) 163
MboII GAAGA 2 cut(s) 137, 395
MflI RGATCY 1 cut(s) 163
MnlI CCTC 3 cut(s) 130, 226, 339
MroNI GCCGGC 1 cut(s) 319
MseI TTAA 1 cut(s) 173
MspI CCGG 2 cut(s) 213, 320
MspR9I CCNGG 1 cut(s) 213
Mva1269I GAATGC 1 cut(s) 134
MwoI GCNNNNNNNGC 3 cut(s) 39, 325, 359
NaeI GCCGGC 1 cut(s) 321
NciI CCSGG 1 cut(s) 213
NcoI CCATGG 1 cut(s) 74
NdeII GATC 1 cut(s) 163
NgoMIV GCCGGC 1 cut(s) 319
NlaIII CATG 2 cut(s) 78, 248
NlaIV GGNNCC 1 cut(s) 165
PaqCI CACCTGC 1 cut(s) 367
PctI GAATGC 1 cut(s) 134
PdiI GCCGGC 1 cut(s) 321
PfeI GAWTC 2 cut(s) 70, 365
PfoI TCCNGGA 1 cut(s) 211
PkrI GCNGC 2 cut(s) 32, 58
PspN4I GGNNCC 1 cut(s) 165
PspPI GGNCC 1 cut(s) 178
PsuI RGATCY 1 cut(s) 163
RsaI GTAC 2 cut(s) 53, 227
RsaNI GTAC 2 cut(s) 52, 226
SaqAI TTAA 1 cut(s) 173
SatI GCNGC 2 cut(s) 31, 57
Sau3AI GATC 1 cut(s) 163
Sau96I GGNCC 1 cut(s) 178
ScrFI CCNGG 1 cut(s) 213
SetI ASST 3 cut(s) 227, 298, 361
SfaNI GCATC 2 cut(s) 19, 94
SinI GGWCC 1 cut(s) 178
SsiI CCGC 5 cut(s) 8, 30, 107, 264, 351
StyD4I CCNGG 1 cut(s) 211
StyI CCWWGG 1 cut(s) 74
TaaI ACNGT 1 cut(s) 206
TaqI TCGA 1 cut(s) 13
TauI GCSGC 1 cut(s) 33
TfiI GAWTC 2 cut(s) 70, 365
Tru1I TTAA 1 cut(s) 173
Tru9I TTAA 1 cut(s) 173
TseI GCWGC 1 cut(s) 56
TspDTI ATGAA 1 cut(s) 233
TspGWI ACGGA 1 cut(s) 15
VpaK11BI GGWCC 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.