pycom05g29220

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0005442
Physical Location & Seq
Forward (+)
29704535 .. 29706231
1697 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g29220.6

Sequence Viewer

Length: 363 bp
ATGACACCGCATCAATGGGGGTCTCCGTGCGGCCACCAATGCACGCACAAGTACGCAGCTCTTATGCAGATTCCATGGCGAGTATTTTGCAAAAAGGGGTGCGATGCGGATGGAGAGACTTGGGAAGAATCTTGGCTCTTATCGTTTCTGAATCTGAAACTTGATATGGAAGTAGACTATTGGTTTAGAGTTAGTACGCTACTAGATCGAGGCTTGGAGGAGTGCAATGAGATATGCTATAAGGATCCTGTCTTAAAGGACCAGCAATGGAGTGCTTGCATTGACCGTTCTCCGGGAGTTGACAGGTACTCAGAGGAGTGTTTTCATGCTTGTGTATCTGGTTGCGGTTTCAAGGCCGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

121

Amino Acids

13.85

Weight (kDa)

5.09

Isoelectric Point (pI)

43.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 174
AciI CCGC 5 cut(s) 8, 30, 107, 345, 357
AclWI GGATC 2 cut(s) 239, 252
AcoI YGGCCR 1 cut(s) 31
AfaI GTAC 3 cut(s) 53, 196, 308
AfiI CCNNNNNNNGG 1 cut(s) 292
AgsI TTSAA 1 cut(s) 352
AluBI AGCT 1 cut(s) 59
AluI AGCT 1 cut(s) 59
Alw26I GTCTC 2 cut(s) 27, 110
AlwI GGATC 2 cut(s) 239, 252
AoxI GGCC 2 cut(s) 31, 354
ApeKI GCWGC 1 cut(s) 56
AspS9I GGNCC 1 cut(s) 259
AsuC2I CCSGG 1 cut(s) 294
AvaII GGWCC 1 cut(s) 259
BamHI GGATCC 1 cut(s) 244
BbvI GCAGC 1 cut(s) 68
BccI CCATC 1 cut(s) 104
BcgI CGANNNNNNTGC 2 cut(s) 69, 103
BcnI CCSGG 1 cut(s) 294
BcoDI GTCTC 2 cut(s) 27, 110
BfaI CTAG 1 cut(s) 203
BisI GCNGC 3 cut(s) 31, 57, 357
BlsI GCNGC 3 cut(s) 32, 58, 358
Bme1390I CCNGG 1 cut(s) 294
Bme18I GGWCC 1 cut(s) 259
BmgT120I GGNCC 1 cut(s) 259
BmiI GGNNCC 1 cut(s) 246
BmrFI CCNGG 1 cut(s) 294
BmsI GCATC 2 cut(s) 19, 94
BpuMI CCSGG 1 cut(s) 294
BsaI GGTCTC 1 cut(s) 27
BsaJI CCNNGG 1 cut(s) 74
Bsc4I CCNNNNNNNGG 1 cut(s) 292
Bse3DI GCAATG 2 cut(s) 232, 272
BseDI CCNNGG 1 cut(s) 74
BseGI GGATG 1 cut(s) 115
BseLI CCNNNNNNNGG 1 cut(s) 292
BseMI GCAATG 2 cut(s) 232, 272
BseMII CTCAG 1 cut(s) 324
BseRI GAGGAG 2 cut(s) 233, 329
BseXI GCAGC 1 cut(s) 68
BshFI GGCC 2 cut(s) 33, 356
BsiSI CCGG 1 cut(s) 293
BslI CCNNNNNNNGG 1 cut(s) 292
BsmAI GTCTC 2 cut(s) 27, 110
BsnI GGCC 2 cut(s) 33, 356
Bso31I GGTCTC 1 cut(s) 27
Bsp143I GATC 2 cut(s) 205, 244
Bsp19I CCATGG 1 cut(s) 74
BspACI CCGC 5 cut(s) 8, 30, 107, 345, 357
BspANI GGCC 2 cut(s) 33, 356
BspCNI CTCAG 1 cut(s) 323
BspLI GGNNCC 1 cut(s) 246
BspPI GGATC 2 cut(s) 239, 252
BspTNI GGTCTC 1 cut(s) 27
BsrDI GCAATG 2 cut(s) 232, 272
BssECI CCNNGG 1 cut(s) 74
BssMI GATC 2 cut(s) 205, 244
BssT1I CCWWGG 1 cut(s) 74
Bst4CI ACNGT 1 cut(s) 287
BstC8I GCNNGC 2 cut(s) 44, 277
BstDEI CTNAG 1 cut(s) 310
BstDSI CCRYGG 1 cut(s) 74
BstF5I GGATG 1 cut(s) 115
BstKTI GATC 2 cut(s) 208, 247
BstMAI GTCTC 2 cut(s) 27, 110
BstMBI GATC 2 cut(s) 205, 244
BstMWI GCNNNNNNNGC 1 cut(s) 39
BstSCI CCNGG 1 cut(s) 292
BstV1I GCAGC 1 cut(s) 68
BstX2I RGATCY 1 cut(s) 244
BstYI RGATCY 1 cut(s) 244
BsuRI GGCC 2 cut(s) 33, 356
BtgI CCRYGG 1 cut(s) 74
BtgZI GCGATG 1 cut(s) 117
BtsCI GGATG 1 cut(s) 115
Cac8I GCNNGC 2 cut(s) 44, 277
Cfr13I GGNCC 1 cut(s) 259
Csp6I GTAC 3 cut(s) 52, 195, 307
CviAII CATG 2 cut(s) 75, 326
CviJI RGCY 5 cut(s) 33, 59, 136, 213, 356
CviKI_1 RGCY 5 cut(s) 33, 59, 136, 213, 356
CviQI GTAC 3 cut(s) 52, 195, 307
DdeI CTNAG 1 cut(s) 310
DpnI GATC 2 cut(s) 207, 246
DpnII GATC 2 cut(s) 205, 244
EaeI YGGCCR 1 cut(s) 31
Eco130I CCWWGG 1 cut(s) 74
Eco31I GGTCTC 1 cut(s) 27
Eco47I GGWCC 1 cut(s) 259
EcoT14I CCWWGG 1 cut(s) 74
ErhI CCWWGG 1 cut(s) 74
FaeI CATG 2 cut(s) 78, 329
FaiI YATR 6 cut(s) 65, 76, 167, 235, 240, 327
FatI CATG 2 cut(s) 74, 325
FblI GTMKAC 1 cut(s) 174
Fnu4HI GCNGC 3 cut(s) 31, 57, 357
FokI GGATG 1 cut(s) 122
Fsp4HI GCNGC 3 cut(s) 31, 57, 357
FspBI CTAG 1 cut(s) 203
GluI GCNGC 3 cut(s) 31, 57, 357
HaeIII GGCC 2 cut(s) 33, 356
HapII CCGG 1 cut(s) 293
Hin1II CATG 2 cut(s) 78, 329
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HinfI GANTC 3 cut(s) 70, 128, 151
HpaII CCGG 1 cut(s) 293
Hpy166II GTNNAC 2 cut(s) 175, 301
Hpy188I TCNGA 3 cut(s) 150, 156, 313
Hpy8I GTNNAC 2 cut(s) 175, 301
HpyCH4III ACNGT 1 cut(s) 287
HpyCH4V TGCA 5 cut(s) 42, 67, 90, 225, 279
HpyF10VI GCNNNNNNNGC 1 cut(s) 39
HpyF3I CTNAG 1 cut(s) 310
Hsp92II CATG 2 cut(s) 78, 329
Kzo9I GATC 2 cut(s) 205, 244
LpnPI CCDG 5 cut(s) 261, 275, 289, 306, 324
Lsp1109I GCAGC 1 cut(s) 68
LweI GCATC 2 cut(s) 19, 94
MaeI CTAG 1 cut(s) 203
MalI GATC 2 cut(s) 207, 246
MboI GATC 2 cut(s) 205, 244
MboII GAAGA 1 cut(s) 137
MflI RGATCY 1 cut(s) 244
MnlI CCTC 3 cut(s) 203, 211, 307
MseI TTAA 1 cut(s) 254
MslI CAYNNNNRTG 1 cut(s) 330
MspI CCGG 1 cut(s) 293
MspR9I CCNGG 1 cut(s) 294
MwoI GCNNNNNNNGC 1 cut(s) 39
NciI CCSGG 1 cut(s) 294
NcoI CCATGG 1 cut(s) 74
NdeII GATC 2 cut(s) 205, 244
NlaIII CATG 2 cut(s) 78, 329
NlaIV GGNNCC 1 cut(s) 246
PfeI GAWTC 3 cut(s) 70, 128, 151
PfoI TCCNGGA 1 cut(s) 292
PkrI GCNGC 3 cut(s) 32, 58, 358
PspN4I GGNNCC 1 cut(s) 246
PspPI GGNCC 1 cut(s) 259
PsuI RGATCY 1 cut(s) 244
RsaI GTAC 3 cut(s) 53, 196, 308
RsaNI GTAC 3 cut(s) 52, 195, 307
RseI CAYNNNNRTG 1 cut(s) 330
SaqAI TTAA 1 cut(s) 254
SatI GCNGC 3 cut(s) 31, 57, 357
Sau3AI GATC 2 cut(s) 205, 244
Sau96I GGNCC 1 cut(s) 259
ScrFI CCNGG 1 cut(s) 294
SetI ASST 2 cut(s) 61, 308
SfaNI GCATC 2 cut(s) 19, 94
SinI GGWCC 1 cut(s) 259
SmiMI CAYNNNNRTG 1 cut(s) 330
SsiI CCGC 5 cut(s) 8, 30, 107, 345, 357
SspMI CTAG 1 cut(s) 203
StyD4I CCNGG 1 cut(s) 292
StyI CCWWGG 1 cut(s) 74
TaaI ACNGT 1 cut(s) 287
TaqI TCGA 1 cut(s) 208
TauI GCSGC 2 cut(s) 33, 359
TfiI GAWTC 3 cut(s) 70, 128, 151
Tru1I TTAA 1 cut(s) 254
Tru9I TTAA 1 cut(s) 254
TseI GCWGC 1 cut(s) 56
TspDTI ATGAA 1 cut(s) 314
TspGWI ACGGA 1 cut(s) 15
VpaK11BI GGWCC 1 cut(s) 259
XmiI GTMKAC 1 cut(s) 174
XspI CTAG 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.