Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0015507

Physical Location & Seq

Genomic Coordinates Reverse (-)

38396113 .. 38398134

Gene Structure 2022 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1295900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 987 bp

ATGGGAAGAGGGGGTGAGAGAGAGGCAGCAGAAGAGGAGGGAGCGATGGCTACAGACTTCTTCTGGTCCTACACGGATGAGCCTCACGCTTCCAGGCGCCGTCAGATCCTCTCTCAGTACCCTCAGATTAAGGAGCTCTTTGGTCCCGACCCCTGGGCTTTCCTCAAGATTACGGTGGTTGTGTTACTTCAGCTCGGGACTGCTACAGCCCTCCACAATGCAGGCTGGCTGAAGATACTAGCGATAGCCTACTTTTTTGGCTCGTTTCTCAACCACAACCTCTTCTTGGCCATCCATGAGCTCAGTCACAATCTCGCCTTCTCGACCCCAGTCTACAACCGTTGGCTCGGTATTTTTGCTAACCTCCCGATTGGTGTTCCCATGTCCGTCACCTTTCAAAAATATCACCTTGAGCACCACCGCTTCCAGGGAGTAGACGGCATTGATATGGACATCCCAAGCAAGGTTGAGGCTCATGTGGTGACAAATGTGGTCGCGAAATCCATTTGGGTTCTCCTTCAACTCTTCTTCTATGCCCTTCGTCCTGTGTTTCTCAAACCGAAACCCCCTGGTTACTGGGAGTTCCTCAACCTCTTCATTCAGGTAGCCCTCGATGTTGCAATGGTCTACTTTTGCGGCTGGAAATCTTTTGCTTACTTGATCCTCTCTACATTTGTCGGCGGTGGGATGCACCCAATGGCGGGTCACTTCATTTCGGAGCACTATGTCTTCAACCCTGATCAAGAGACGTATTCTTACTATGGCCCTCTCAATCTCCTAGCTTGGCACGTAGGCTACCACAACGAGCACCATGATTTCCCCAGAATTCCCGGGAGCAAGCTTCATAAGGTGAAGGACATTGCACCGGAATACTACGAGGGTTTGGAGTCGTATAAATCTTGGAGCCAAGTGATCTACATGTACATAGCGGATCGAACAGTTGGACCGTTCAGTCGAATGAAGAGAAAGGCAACAAAATCTGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0006629 GO:0006643 GO:0006665 GO:0006672 GO:0006807 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009987 GO:0016491 GO:0016705 GO:0030148 GO:0034641 GO:0042284 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044271 GO:0046467 GO:0046513 GO:0055114 GO:0071704 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00600 ko01100 ko04071 map00600 map01100 map04071

Pfam Domains

Protein Families

FA_desaturase Lipid_DES

Protein Analysis

329

Amino Acids

37.73

Weight (kDa)

7.0

Isoelectric Point (pI)

40.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lipid_DES	PF08557	17 - 52	3.3e-20	Sphingolipid Delta4-desaturase (DES)
FA_desaturase	PF00487	75 - 293	3.4e-23	Fatty acid desaturase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015507)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G04930
fragaria_vesca	FvH4_3g04930
malus_domestica	MD10G1295900.v1.1
prunus_persica	Prupe.4G044600_v2.0.a1
pyrus_communis	pycom10g24850
rosa_chinensis	RchiOBHm_Chr5g0008091
rosa_laevigata	RLG00000031535
rosa_multiflora	Rmu_sc0017465.1_g000005 Rmu_sc0039673.1_g000001
rosa_roxburghii	Rroxscaffold_1G00068390
rosa_rugosa	Rorug04G0433300
rosa_samantha	Rh5AG061400 Rh5BG058200 Rh5CG068300 Rh5DG057500
rosa_wichuraiana	Rw5G005920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	951
AccB1I	GGYRCC	1 cut(s)	96
AccI	GTMKAC	3 cut(s)	333, 435, 627
AccII	CGCG	1 cut(s)	497
AciI	CCGC	5 cut(s)	421, 636, 681, 701, 929
AclWI	GGATC	3 cut(s)	100, 655, 939
AcoI	YGGCCR	1 cut(s)	288
AcsI	RAATTY	1 cut(s)	825
AcuI	CTGAAG	2 cut(s)	173, 251
AcyI	GRCGYC	1 cut(s)	97
AfaI	GTAC	2 cut(s)	119, 923
AfiI	CCNNNNNNNGG	6 cut(s)	153, 286, 427, 463, 700, 701
AflIII	ACRYGT	1 cut(s)	918
AgsI	TTSAA	3 cut(s)	398, 521, 733
AjnI	CCWGG	4 cut(s)	92, 152, 426, 568
AluBI	AGCT	5 cut(s)	136, 193, 301, 782, 841
AluI	AGCT	5 cut(s)	136, 193, 301, 782, 841
Alw21I	GWGCWC	5 cut(s)	138, 303, 417, 723, 810
Alw26I	GTCTC	1 cut(s)	740
AlwI	GGATC	3 cut(s)	100, 655, 939
Ama87I	CYCGRG	2 cut(s)	194, 830
AoxI	GGCC	2 cut(s)	288, 763
ApeKI	GCWGC	1 cut(s)	26
ApoI	RAATTY	1 cut(s)	825
AspLEI	GCGC	1 cut(s)	99
AspS9I	GGNCC	4 cut(s)	66, 143, 764, 944
AsuC2I	CCSGG	2 cut(s)	831, 832
AsuHPI	GGTGA	5 cut(s)	26, 382, 398, 493, 862
AvaI	CYCGRG	2 cut(s)	194, 830
AvaII	GGWCC	3 cut(s)	66, 143, 944
BalI	TGGCCA	1 cut(s)	290
BanI	GGYRCC	1 cut(s)	96
BanII	GRGCYC	2 cut(s)	138, 303
BbsI	GAAGAC	1 cut(s)	721
Bbv12I	GWGCWC	5 cut(s)	138, 303, 417, 723, 810
BbvI	GCAGC	1 cut(s)	38
BccI	CCATC	2 cut(s)	40, 299
BceAI	ACGGC	2 cut(s)	84, 454
BciT130I	CCWGG	4 cut(s)	94, 154, 428, 570
BclI	TGATCA	1 cut(s)	739
BcnI	CCSGG	2 cut(s)	831, 832
BcoDI	GTCTC	1 cut(s)	740
BfaI	CTAG	2 cut(s)	239, 779
BfmI	CTRYAG	2 cut(s)	51, 204
BfoI	RGCGCY	1 cut(s)	100
BisI	GCNGC	2 cut(s)	27, 637
BlsI	GCNGC	2 cut(s)	28, 638
Bme1390I	CCNGG	6 cut(s)	94, 154, 428, 570, 831, 832
Bme18I	GGWCC	3 cut(s)	66, 143, 944
BmeT110I	CYCGRG	2 cut(s)	194, 830
BmgT120I	GGNCC	4 cut(s)	66, 143, 764, 944
BmiI	GGNNCC	3 cut(s)	98, 145, 905
BmrFI	CCNGG	6 cut(s)	94, 154, 428, 570, 831, 832
BmrI	ACTGGG	2 cut(s)	323, 586
BmsI	GCATC	1 cut(s)	678
BmuI	ACTGGG	2 cut(s)	323, 586
BoxI	GACNNNNGTC	1 cut(s)	329
BpiI	GAAGAC	1 cut(s)	721
BpuEI	CTTGAG	2 cut(s)	149, 431
BpuMI	CCSGG	2 cut(s)	831, 832
BsaAI	YACGTR	1 cut(s)	790
BsaHI	GRCGYC	1 cut(s)	97
BsaJI	CCNNGG	5 cut(s)	152, 153, 427, 568, 830
BsaWI	WCCGGW	1 cut(s)	865
Bsc4I	CCNNNNNNNGG	6 cut(s)	153, 286, 427, 463, 700, 701
Bse1I	ACTGG	2 cut(s)	329, 581
Bse3DI	GCAATG	2 cut(s)	627, 858
BseBI	CCWGG	4 cut(s)	94, 154, 428, 570
BseDI	CCNNGG	5 cut(s)	152, 153, 427, 568, 830
BseGI	GGATG	4 cut(s)	82, 291, 453, 693
BseLI	CCNNNNNNNGG	6 cut(s)	153, 286, 427, 463, 700, 701
BseMI	GCAATG	2 cut(s)	627, 858
BseMII	CTCAG	3 cut(s)	128, 137, 316
BseNI	ACTGG	2 cut(s)	329, 581
BseRI	GAGGAG	1 cut(s)	50
BseXI	GCAGC	1 cut(s)	38
Bsh1236I	CGCG	1 cut(s)	497
BshFI	GGCC	2 cut(s)	290, 765
BshNI	GGYRCC	1 cut(s)	96
BsiHKAI	GWGCWC	5 cut(s)	138, 303, 417, 723, 810
BsiHKCI	CYCGRG	2 cut(s)	194, 830
BsiSI	CCGG	2 cut(s)	831, 866
BslFI	GGGAC	2 cut(s)	129, 211
BslI	CCNNNNNNNGG	6 cut(s)	153, 286, 427, 463, 700, 701
BsmAI	GTCTC	1 cut(s)	740
BsmBI	CGTCTC	1 cut(s)	740
BsmFI	GGGAC	2 cut(s)	129, 211
BsnI	GGCC	2 cut(s)	290, 765
BsoBI	CYCGRG	2 cut(s)	194, 830
Bsp1286I	GDGCHC	5 cut(s)	138, 303, 417, 723, 810
Bsp1407I	TGTACA	1 cut(s)	921
Bsp143I	GATC	5 cut(s)	105, 660, 739, 912, 931
Bsp68I	TCGCGA	1 cut(s)	497
BspACI	CCGC	5 cut(s)	421, 636, 681, 701, 929
BspANI	GGCC	2 cut(s)	290, 765
BspCNI	CTCAG	3 cut(s)	127, 136, 315
BspFNI	CGCG	1 cut(s)	497
BspLI	GGNNCC	3 cut(s)	98, 145, 905
BspPI	GGATC	3 cut(s)	100, 655, 939
BspT107I	GGYRCC	1 cut(s)	96
BsrDI	GCAATG	2 cut(s)	627, 858
BsrGI	TGTACA	1 cut(s)	921
BsrI	ACTGG	2 cut(s)	329, 581
BssECI	CCNNGG	5 cut(s)	152, 153, 427, 568, 830
BssMI	GATC	5 cut(s)	105, 660, 739, 912, 931
BssNI	GRCGYC	1 cut(s)	97
Bst2UI	CCWGG	4 cut(s)	94, 154, 428, 570
Bst4CI	ACNGT	4 cut(s)	175, 341, 940, 948
Bst6I	CTCTTC	5 cut(s)	27, 287, 530, 599, 956
BstACI	GRCGYC	1 cut(s)	97
BstAUI	TGTACA	1 cut(s)	921
BstBAI	YACGTR	1 cut(s)	790
BstC8I	GCNNGC	3 cut(s)	223, 227, 839
BstDEI	CTNAG	3 cut(s)	114, 123, 302
BstF5I	GGATG	4 cut(s)	82, 291, 453, 693
BstFNI	CGCG	1 cut(s)	497
BstH2I	RGCGCY	1 cut(s)	100
BstHHI	GCGC	1 cut(s)	99
BstKTI	GATC	5 cut(s)	108, 663, 742, 915, 934
BstMAI	GTCTC	1 cut(s)	740
BstMBI	GATC	5 cut(s)	105, 660, 739, 912, 931
BstNI	CCWGG	4 cut(s)	94, 154, 428, 570
BstNSI	RCATGY	1 cut(s)	922
BstPAI	GACNNNNGTC	1 cut(s)	329
BstSCI	CCNGG	6 cut(s)	92, 152, 426, 568, 829, 830
BstSFI	CTRYAG	2 cut(s)	51, 204
BstUI	CGCG	1 cut(s)	497
BstV1I	GCAGC	1 cut(s)	38
BstV2I	GAAGAC	1 cut(s)	721
BstX2I	RGATCY	1 cut(s)	105
BstYI	RGATCY	1 cut(s)	105
BsuRI	GGCC	2 cut(s)	290, 765
BtgZI	GCGATG	1 cut(s)	59
BtsCI	GGATG	4 cut(s)	82, 291, 453, 693
BtuMI	TCGCGA	1 cut(s)	497
Cac8I	GCNNGC	3 cut(s)	223, 227, 839
CfoI	GCGC	1 cut(s)	99
Cfr13I	GGNCC	4 cut(s)	66, 143, 764, 944
Cfr9I	CCCGGG	1 cut(s)	830
Csp6I	GTAC	2 cut(s)	118, 922
CviAII	CATG	5 cut(s)	296, 382, 476, 812, 919
CviQI	GTAC	2 cut(s)	118, 922
DdeI	CTNAG	3 cut(s)	114, 123, 302
DinI	GGCGCC	1 cut(s)	98
DpnI	GATC	5 cut(s)	107, 662, 741, 914, 933
DpnII	GATC	5 cut(s)	105, 660, 739, 912, 931
DrdI	GACNNNNNNGTC	1 cut(s)	951
DseDI	GACNNNNNNGTC	1 cut(s)	951
EaeI	YGGCCR	1 cut(s)	288
Eam1104I	CTCTTC	5 cut(s)	27, 287, 530, 599, 956
EarI	CTCTTC	5 cut(s)	27, 287, 530, 599, 956
Ecl136II	GAGCTC	2 cut(s)	136, 301
Eco24I	GRGCYC	2 cut(s)	138, 303
Eco47I	GGWCC	3 cut(s)	66, 143, 944
Eco53kI	GAGCTC	2 cut(s)	136, 301
Eco57I	CTGAAG	2 cut(s)	173, 251
Eco88I	CYCGRG	2 cut(s)	194, 830
EcoICRI	GAGCTC	2 cut(s)	136, 301
EcoRI	GAATTC	1 cut(s)	825
EcoRII	CCWGG	4 cut(s)	92, 152, 426, 568
EcoT38I	GRGCYC	2 cut(s)	138, 303
EgeI	GGCGCC	1 cut(s)	98
EheI	GGCGCC	1 cut(s)	98
Esp3I	CGTCTC	1 cut(s)	740
FaeI	CATG	5 cut(s)	299, 385, 479, 815, 922
FalI	AAGNNNNNCTT	2 cut(s)	122, 154
FaqI	GGGAC	2 cut(s)	129, 211
FatI	CATG	5 cut(s)	295, 381, 475, 811, 918
FauI	CCCGC	1 cut(s)	694
FbaI	TGATCA	1 cut(s)	739
FblI	GTMKAC	3 cut(s)	333, 435, 627
Fnu4HI	GCNGC	2 cut(s)	27, 637
FokI	GGATG	4 cut(s)	89, 278, 440, 700
FriOI	GRGCYC	2 cut(s)	138, 303
Fsp4HI	GCNGC	2 cut(s)	27, 637
FspBI	CTAG	2 cut(s)	239, 779
GlaI	GCGC	1 cut(s)	98
GluI	GCNGC	2 cut(s)	27, 637
HaeII	RGCGCY	1 cut(s)	100
HaeIII	GGCC	2 cut(s)	290, 765
HapII	CCGG	2 cut(s)	831, 866
HhaI	GCGC	1 cut(s)	99
Hin1I	GRCGYC	1 cut(s)	97
Hin1II	CATG	5 cut(s)	299, 385, 479, 815, 922
Hin6I	GCGC	1 cut(s)	97
HinP1I	GCGC	1 cut(s)	97
HindIII	AAGCTT	1 cut(s)	839
HinfI	GANTC	1 cut(s)	887
HpaII	CCGG	2 cut(s)	831, 866
HphI	GGTGA	5 cut(s)	26, 382, 398, 493, 862
Hpy166II	GTNNAC	3 cut(s)	334, 436, 628
Hpy188I	TCNGA	4 cut(s)	105, 126, 718, 982
Hpy188III	TCNNGA	7 cut(s)	146, 166, 196, 322, 367, 496, 743
Hpy8I	GTNNAC	3 cut(s)	334, 436, 628
HpyAV	CCTTC	4 cut(s)	328, 527, 548, 847
HpyCH4III	ACNGT	4 cut(s)	175, 341, 940, 948
HpyCH4IV	ACGT	2 cut(s)	749, 789
HpyCH4V	TGCA	4 cut(s)	221, 620, 691, 863
HpyF3I	CTNAG	3 cut(s)	114, 123, 302
HpySE526I	ACGT	2 cut(s)	749, 789
Hsp92I	GRCGYC	1 cut(s)	97
Hsp92II	CATG	5 cut(s)	299, 385, 479, 815, 922
HspAI	GCGC	1 cut(s)	97
KasI	GGCGCC	1 cut(s)	96
Ksp22I	TGATCA	1 cut(s)	739
Kzo9I	GATC	5 cut(s)	105, 660, 739, 912, 931
LmnI	GCTCC	5 cut(s)	41, 133, 718, 834, 903
Lsp1109I	GCAGC	1 cut(s)	38
LweI	GCATC	1 cut(s)	678
MaeI	CTAG	2 cut(s)	239, 779
MaeII	ACGT	2 cut(s)	749, 789
MaeIII	GTNAC	6 cut(s)	183, 305, 388, 481, 572, 704
MalI	GATC	5 cut(s)	107, 662, 741, 914, 933
MboI	GATC	5 cut(s)	105, 660, 739, 912, 931
MflI	RGATCY	1 cut(s)	105
MhlI	GDGCHC	5 cut(s)	138, 303, 417, 723, 810
MlsI	TGGCCA	1 cut(s)	290
MluCI	AATT	1 cut(s)	825
MluNI	TGGCCA	1 cut(s)	290
Mly113I	GGCGCC	1 cut(s)	97
MlyI	GAGTC	1 cut(s)	896
MmeI	TCCRAC	1 cut(s)	922
Mox20I	TGGCCA	1 cut(s)	290
MscI	TGGCCA	1 cut(s)	290
MseI	TTAA	1 cut(s)	129
MslI	CAYNNNNRTG	1 cut(s)	446
Msp20I	TGGCCA	1 cut(s)	290
MspI	CCGG	2 cut(s)	831, 866
MspR9I	CCNGG	6 cut(s)	94, 154, 428, 570, 831, 832
MvaI	CCWGG	4 cut(s)	94, 154, 428, 570
MvnI	CGCG	1 cut(s)	497
NarI	GGCGCC	1 cut(s)	97
NciI	CCSGG	2 cut(s)	831, 832
NdeII	GATC	5 cut(s)	105, 660, 739, 912, 931
NlaIII	CATG	5 cut(s)	299, 385, 479, 815, 922
NlaIV	GGNNCC	3 cut(s)	98, 145, 905
NmuCI	GTSAC	4 cut(s)	305, 388, 481, 704
NruI	TCGCGA	1 cut(s)	497
NspI	RCATGY	1 cut(s)	922
PasI	CCCWGGG	1 cut(s)	153
PciI	ACATGT	1 cut(s)	918
PkrI	GCNGC	2 cut(s)	28, 638
PleI	GAGTC	1 cut(s)	895
PluTI	GGCGCC	1 cut(s)	100
PpsI	GAGTC	1 cut(s)	895
Ppu21I	YACGTR	1 cut(s)	790
PscI	ACATGT	1 cut(s)	918
PshAI	GACNNNNGTC	1 cut(s)	329
Psp124BI	GAGCTC	2 cut(s)	138, 303
Psp6I	CCWGG	4 cut(s)	92, 152, 426, 568
PspGI	CCWGG	4 cut(s)	92, 152, 426, 568
PspN4I	GGNNCC	3 cut(s)	98, 145, 905
PspPI	GGNCC	4 cut(s)	66, 143, 764, 944
PsuI	RGATCY	1 cut(s)	105
RruI	TCGCGA	1 cut(s)	497
RsaI	GTAC	2 cut(s)	119, 923
RsaNI	GTAC	2 cut(s)	118, 922
RseI	CAYNNNNRTG	1 cut(s)	446
SacI	GAGCTC	2 cut(s)	138, 303
SaqAI	TTAA	1 cut(s)	129
SatI	GCNGC	2 cut(s)	27, 637
Sau3AI	GATC	5 cut(s)	105, 660, 739, 912, 931
Sau96I	GGNCC	4 cut(s)	66, 143, 764, 944
SchI	GAGTC	1 cut(s)	896
ScrFI	CCNGG	6 cut(s)	94, 154, 428, 570, 831, 832
SduI	GDGCHC	5 cut(s)	138, 303, 417, 723, 810
SfaNI	GCATC	1 cut(s)	678
SfcI	CTRYAG	2 cut(s)	51, 204
SfoI	GGCGCC	1 cut(s)	98
SinI	GGWCC	3 cut(s)	66, 143, 944
SmaI	CCCGGG	1 cut(s)	832
SmiMI	CAYNNNNRTG	1 cut(s)	446
SmlI	CTYRAG	2 cut(s)	164, 410
SmoI	CTYRAG	2 cut(s)	164, 410
Sse9I	AATT	1 cut(s)	825
SsiI	CCGC	5 cut(s)	421, 636, 681, 701, 929
SspDI	GGCGCC	1 cut(s)	96
SspMI	CTAG	2 cut(s)	239, 779
SstI	GAGCTC	2 cut(s)	138, 303
StyD4I	CCNGG	6 cut(s)	92, 152, 426, 568, 829, 830
TaaI	ACNGT	4 cut(s)	175, 341, 940, 948
TaiI	ACGT	2 cut(s)	752, 792
TaqI	TCGA	4 cut(s)	323, 612, 934, 955
TasI	AATT	1 cut(s)	825
TatI	WGTACW	1 cut(s)	921
TauI	GCSGC	1 cut(s)	639
Tru1I	TTAA	1 cut(s)	129
Tru9I	TTAA	1 cut(s)	129
TseFI	GTSAC	4 cut(s)	305, 388, 481, 704
TseI	GCWGC	1 cut(s)	26
Tsp45I	GTSAC	4 cut(s)	305, 388, 481, 704
TspDTI	ATGAA	4 cut(s)	586, 700, 833, 974
TspGWI	ACGGA	2 cut(s)	89, 376
TspMI	CCCGGG	1 cut(s)	830
VpaK11BI	GGWCC	3 cut(s)	66, 143, 944
XapI	RAATTY	1 cut(s)	825
XceI	RCATGY	1 cut(s)	922
XmaI	CCCGGG	1 cut(s)	830
XmiI	GTMKAC	3 cut(s)	333, 435, 627
XspI	CTAG	2 cut(s)	239, 779

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.