Rh5BG058200

Sphingolipid delta(4)-desaturase DES1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0015507
Physical Location & Seq
Forward (+)
4769436 .. 4773856
4421 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG058200.1

Sequence Viewer

Length: 825 bp
ATGTGGATTACTGTGGTTGTGTTGCTTCAGCTCTGCACTGCTGCAGCGCTTCACAATGCAGGCTGGCTGAAGATTCTGGCAATAGCCTACTTCTTCGGCTCTTTTCTCAACCACAACCTCTTCTTGGCCATCCATGAGCTCAGCCACAATCTCGCCTTCTCAACCCCAGTCTACAACCGTTGGCTTGGTATCTTTGCTAACCTCCCAATTGGTGTTCCCATGTCTGTCACCTTCCAGAAGTATCACCTCGAGCACCATCGCTTCCAAGGAGTAGATGGCGTTGACATGGACATCCCAAGCAAAGTTGAGGCTTATGTGGTGAGAAATGTGTTCACCAAATCCATATGGGTTCTACTTCAACTCTTCTTCTATGCTCTTCGGCCTGTGTTTCTGAAACCAAAACCCCCTGGTTGTTGGGAGTTCATCAACCTTTTTGTCCAGATATCCCTCGATGTCTCCATGGTTTACTTCTGTGGTTGGAAGTCTTTTGCTTATTTGATCCTCTCTACATTTGTTGGGGGTGGGATGCACCCTATGGCTGGTCACTTCATTTCCGAGCATTATGTCTTCAATCCCGATCAAGAGACATATTCTTATTATGGCCCTCTTAATCTCATGACTTGGAGCGTAGGGTACCATAACGAACACCACGACTTTCCCAGAATTCCTGGGAGCAAGCTCCACAAGGTGAAGTCCATTGCACCAGAATACTATGAGGGTTTGGAGTCTTATAAATCATGGAGTCAGGTCATCTACATGTATATAATGGACAGAACAGTTGGGCCATTTAGCCGAATGAAGAGGAAGCCAAGCAAATCGGAATAG

Protein Analysis

274

Amino Acids

31.74

Weight (kDa)

8.81

Isoelectric Point (pI)

41.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase PF00487 21 - 239 5.9e-24 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015507)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G04930
fragaria_vesca FvH4_3g04930
malus_domestica MD10G1295900.v1.1
prunus_persica Prupe.4G044600_v2.0.a1
pyrus_communis pycom10g24850
rosa_chinensis RchiOBHm_Chr5g0008091
rosa_laevigata RLG00000031535
rosa_multiflora Rmu_sc0017465.1_g000005 Rmu_sc0039673.1_g000001
rosa_roxburghii Rroxscaffold_1G00068390
rosa_rugosa Rorug04G0433300
rosa_samantha Rh5AG061400 Rh5BG058200 Rh5CG068300 Rh5DG057500
rosa_wichuraiana Rw5G005920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 732
Acc65I GGTACC 1 cut(s) 633
AccB1I GGYRCC 1 cut(s) 633
AccI GTMKAC 1 cut(s) 171
AclWI GGATC 1 cut(s) 493
AcoI YGGCCR 1 cut(s) 126
AcsI RAATTY 1 cut(s) 663
AcuI CTGAAG 2 cut(s) 11, 89
AdeI CACNNNGTG 1 cut(s) 688
AfaI GTAC 1 cut(s) 635
AfeI AGCGCT 1 cut(s) 48
AfiI CCNNNNNNNGG 2 cut(s) 124, 539
AflIII ACRYGT 1 cut(s) 756
AgsI TTSAA 2 cut(s) 359, 571
AjnI CCWGG 2 cut(s) 406, 667
AluBI AGCT 3 cut(s) 31, 139, 679
AluI AGCT 3 cut(s) 31, 139, 679
Alw21I GWGCWC 2 cut(s) 141, 255
Alw26I GTCTC 2 cut(s) 460, 578
AlwI GGATC 1 cut(s) 493
Ama87I CYCGRG 1 cut(s) 248
Aor51HI AGCGCT 1 cut(s) 48
AoxI GGCC 4 cut(s) 126, 380, 601, 782
ApeKI GCWGC 2 cut(s) 41, 44
ApoI RAATTY 1 cut(s) 663
Asp718I GGTACC 1 cut(s) 633
AspLEI GCGC 1 cut(s) 49
AspS9I GGNCC 2 cut(s) 602, 782
AsuHPI GGTGA 5 cut(s) 220, 236, 325, 331, 700
AvaI CYCGRG 1 cut(s) 248
BalI TGGCCA 1 cut(s) 128
BanI GGYRCC 1 cut(s) 633
BanII GRGCYC 1 cut(s) 141
BbsI GAAGAC 1 cut(s) 559
Bbv12I GWGCWC 2 cut(s) 141, 255
BbvI GCAGC 2 cut(s) 28, 56
BccI CCATC 3 cut(s) 137, 264, 269
BciT130I CCWGG 2 cut(s) 408, 669
BcoDI GTCTC 2 cut(s) 460, 578
BfmI CTRYAG 1 cut(s) 42
BfoI RGCGCY 1 cut(s) 50
BisI GCNGC 2 cut(s) 42, 45
BlpI GCTNAGC 1 cut(s) 140
BlsI GCNGC 2 cut(s) 43, 46
Bme1390I CCNGG 2 cut(s) 408, 669
BmeT110I CYCGRG 1 cut(s) 248
BmgT120I GGNCC 2 cut(s) 602, 782
BmiI GGNNCC 1 cut(s) 635
BmrFI CCNGG 2 cut(s) 408, 669
BmrI ACTGGG 1 cut(s) 161
BmsI GCATC 1 cut(s) 516
BmuI ACTGGG 1 cut(s) 161
BpiI GAAGAC 1 cut(s) 559
Bpu1102I GCTNAGC 1 cut(s) 140
BsaJI CCNNGG 4 cut(s) 265, 406, 459, 668
Bsc4I CCNNNNNNNGG 2 cut(s) 124, 539
Bse1I ACTGG 1 cut(s) 167
Bse3DI GCAATG 1 cut(s) 696
BseBI CCWGG 2 cut(s) 408, 669
BseDI CCNNGG 4 cut(s) 265, 406, 459, 668
BseGI GGATG 3 cut(s) 129, 291, 531
BseLI CCNNNNNNNGG 2 cut(s) 124, 539
BseMI GCAATG 1 cut(s) 696
BseMII CTCAG 1 cut(s) 154
BseNI ACTGG 1 cut(s) 167
BseXI GCAGC 2 cut(s) 28, 56
BsgI GTGCAG 1 cut(s) 19
BshFI GGCC 4 cut(s) 128, 382, 603, 784
BshNI GGYRCC 1 cut(s) 633
BsiHKAI GWGCWC 2 cut(s) 141, 255
BsiHKCI CYCGRG 1 cut(s) 248
BslI CCNNNNNNNGG 2 cut(s) 124, 539
BsmAI GTCTC 2 cut(s) 460, 578
BsnI GGCC 4 cut(s) 128, 382, 603, 784
BsoBI CYCGRG 1 cut(s) 248
Bsp1286I GDGCHC 2 cut(s) 141, 255
Bsp143I GATC 2 cut(s) 498, 577
Bsp1720I GCTNAGC 1 cut(s) 140
Bsp19I CCATGG 1 cut(s) 459
BspANI GGCC 4 cut(s) 128, 382, 603, 784
BspCNI CTCAG 1 cut(s) 153
BspHI TCATGA 1 cut(s) 615
BspLI GGNNCC 1 cut(s) 635
BspMAI CTGCAG 1 cut(s) 46
BspPI GGATC 1 cut(s) 493
BspQI GCTCTTC 1 cut(s) 381
BspT107I GGYRCC 1 cut(s) 633
BsrDI GCAATG 1 cut(s) 696
BsrI ACTGG 1 cut(s) 167
BssECI CCNNGG 4 cut(s) 265, 406, 459, 668
BssMI GATC 2 cut(s) 498, 577
BssT1I CCWWGG 2 cut(s) 265, 459
Bst2UI CCWGG 2 cut(s) 408, 669
Bst4CI ACNGT 3 cut(s) 13, 179, 778
Bst6I CTCTTC 4 cut(s) 125, 368, 381, 794
BstC8I GCNNGC 3 cut(s) 61, 65, 677
BstDEI CTNAG 1 cut(s) 140
BstDSI CCRYGG 1 cut(s) 459
BstF5I GGATG 3 cut(s) 129, 291, 531
BstH2I RGCGCY 1 cut(s) 50
BstHHI GCGC 1 cut(s) 49
BstKTI GATC 2 cut(s) 501, 580
BstMAI GTCTC 2 cut(s) 460, 578
BstMBI GATC 2 cut(s) 498, 577
BstNI CCWGG 2 cut(s) 408, 669
BstNSI RCATGY 1 cut(s) 760
BstSCI CCNGG 2 cut(s) 406, 667
BstSFI CTRYAG 1 cut(s) 42
BstV1I GCAGC 2 cut(s) 28, 56
BstV2I GAAGAC 1 cut(s) 559
BsuRI GGCC 4 cut(s) 128, 382, 603, 784
BtgI CCRYGG 1 cut(s) 459
BtgZI GCGATG 1 cut(s) 242
BtsCI GGATG 3 cut(s) 129, 291, 531
BtsI GCAGTG 1 cut(s) 36
BtsIMutI CAGTG 1 cut(s) 36
Cac8I GCNNGC 3 cut(s) 61, 65, 677
CciI TCATGA 1 cut(s) 615
CfoI GCGC 1 cut(s) 49
Cfr13I GGNCC 2 cut(s) 602, 782
Csp6I GTAC 1 cut(s) 634
CviAII CATG 7 cut(s) 134, 220, 286, 460, 616, 738, 757
CviQI GTAC 1 cut(s) 634
DdeI CTNAG 1 cut(s) 140
DpnI GATC 2 cut(s) 500, 579
DpnII GATC 2 cut(s) 498, 577
DraIII CACNNNGTG 1 cut(s) 688
EaeI YGGCCR 1 cut(s) 126
Eam1104I CTCTTC 4 cut(s) 125, 368, 381, 794
EarI CTCTTC 4 cut(s) 125, 368, 381, 794
Ecl136II GAGCTC 1 cut(s) 139
Eco130I CCWWGG 2 cut(s) 265, 459
Eco24I GRGCYC 1 cut(s) 141
Eco32I GATATC 1 cut(s) 444
Eco47III AGCGCT 1 cut(s) 48
Eco53kI GAGCTC 1 cut(s) 139
Eco57I CTGAAG 2 cut(s) 11, 89
Eco88I CYCGRG 1 cut(s) 248
EcoICRI GAGCTC 1 cut(s) 139
EcoRI GAATTC 1 cut(s) 663
EcoRII CCWGG 2 cut(s) 406, 667
EcoRV GATATC 1 cut(s) 444
EcoT14I CCWWGG 2 cut(s) 265, 459
EcoT38I GRGCYC 1 cut(s) 141
ErhI CCWWGG 2 cut(s) 265, 459
FaeI CATG 7 cut(s) 137, 223, 289, 463, 619, 741, 760
FatI CATG 7 cut(s) 133, 219, 285, 459, 615, 737, 756
FauNDI CATATG 1 cut(s) 344
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 2 cut(s) 42, 45
FokI GGATG 3 cut(s) 116, 278, 538
FriOI GRGCYC 1 cut(s) 141
Fsp4HI GCNGC 2 cut(s) 42, 45
GlaI GCGC 1 cut(s) 48
GluI GCNGC 2 cut(s) 42, 45
HaeII RGCGCY 1 cut(s) 50
HaeIII GGCC 4 cut(s) 128, 382, 603, 784
HhaI GCGC 1 cut(s) 49
Hin1II CATG 7 cut(s) 137, 223, 289, 463, 619, 741, 760
Hin6I GCGC 1 cut(s) 47
HinP1I GCGC 1 cut(s) 47
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HinfI GANTC 3 cut(s) 73, 725, 742
HphI GGTGA 5 cut(s) 220, 236, 325, 331, 700
Hpy166II GTNNAC 4 cut(s) 172, 283, 333, 466
Hpy188I TCNGA 3 cut(s) 393, 556, 820
Hpy188III TCNNGA 5 cut(s) 235, 439, 575, 581, 616
Hpy8I GTNNAC 4 cut(s) 172, 283, 333, 466
HpyAV CCTTC 2 cut(s) 166, 241
HpyCH4III ACNGT 3 cut(s) 13, 179, 778
HpyCH4V TGCA 5 cut(s) 36, 44, 59, 529, 701
HpyF3I CTNAG 1 cut(s) 140
Hsp92II CATG 7 cut(s) 137, 223, 289, 463, 619, 741, 760
HspAI GCGC 1 cut(s) 47
KpnI GGTACC 1 cut(s) 637
Kzo9I GATC 2 cut(s) 498, 577
LguI GCTCTTC 1 cut(s) 381
LmnI GCTCC 3 cut(s) 624, 672, 684
Lsp1109I GCAGC 2 cut(s) 28, 56
LweI GCATC 1 cut(s) 516
MaeIII GTNAC 2 cut(s) 226, 542
MalI GATC 2 cut(s) 500, 579
MboI GATC 2 cut(s) 498, 577
MboII GAAGA 8 cut(s) 82, 85, 112, 355, 358, 368, 559, 811
MfeI CAATTG 1 cut(s) 207
MhlI GDGCHC 2 cut(s) 141, 255
MlsI TGGCCA 1 cut(s) 128
MluCI AATT 2 cut(s) 207, 663
MluNI TGGCCA 1 cut(s) 128
MlyI GAGTC 2 cut(s) 734, 751
MmeI TCCRAC 1 cut(s) 458
MnlI CCTC 9 cut(s) 128, 212, 257, 301, 458, 512, 615, 709, 795
Mox20I TGGCCA 1 cut(s) 128
MscI TGGCCA 1 cut(s) 128
MseI TTAA 1 cut(s) 609
MslI CAYNNNNRTG 1 cut(s) 755
Msp20I TGGCCA 1 cut(s) 128
MspR9I CCNGG 2 cut(s) 408, 669
MunI CAATTG 1 cut(s) 207
MvaI CCWGG 2 cut(s) 408, 669
NcoI CCATGG 1 cut(s) 459
NdeI CATATG 1 cut(s) 344
NdeII GATC 2 cut(s) 498, 577
NlaIII CATG 7 cut(s) 137, 223, 289, 463, 619, 741, 760
NlaIV GGNNCC 1 cut(s) 635
NmuCI GTSAC 2 cut(s) 226, 542
NspI RCATGY 1 cut(s) 760
PaeR7I CTCGAG 1 cut(s) 248
PagI TCATGA 1 cut(s) 615
PciI ACATGT 1 cut(s) 756
PciSI GCTCTTC 1 cut(s) 381
PcsI WCGNNNNNNNCGW 1 cut(s) 648
PfeI GAWTC 1 cut(s) 73
PkrI GCNGC 2 cut(s) 43, 46
PleI GAGTC 2 cut(s) 733, 750
PpsI GAGTC 2 cut(s) 733, 750
PscI ACATGT 1 cut(s) 756
PsiI TTATAA 1 cut(s) 732
Psp124BI GAGCTC 1 cut(s) 141
Psp6I CCWGG 2 cut(s) 406, 667
PspGI CCWGG 2 cut(s) 406, 667
PspN4I GGNNCC 1 cut(s) 635
PspPI GGNCC 2 cut(s) 602, 782
PspXI VCTCGAGB 1 cut(s) 248
PstI CTGCAG 1 cut(s) 46
RsaI GTAC 1 cut(s) 635
RsaNI GTAC 1 cut(s) 634
RseI CAYNNNNRTG 1 cut(s) 755
SacI GAGCTC 1 cut(s) 141
SapI GCTCTTC 1 cut(s) 381
SaqAI TTAA 1 cut(s) 609
SatI GCNGC 2 cut(s) 42, 45
Sau3AI GATC 2 cut(s) 498, 577
Sau96I GGNCC 2 cut(s) 602, 782
SchI GAGTC 2 cut(s) 734, 751
ScrFI CCNGG 2 cut(s) 408, 669
SduI GDGCHC 2 cut(s) 141, 255
SfaNI GCATC 1 cut(s) 516
SfcI CTRYAG 1 cut(s) 42
Sfr274I CTCGAG 1 cut(s) 248
SlaI CTCGAG 1 cut(s) 248
SmiMI CAYNNNNRTG 1 cut(s) 755
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
Sse9I AATT 2 cut(s) 207, 663
SstI GAGCTC 1 cut(s) 141
StyD4I CCNGG 2 cut(s) 406, 667
StyI CCWWGG 2 cut(s) 265, 459
TaaI ACNGT 3 cut(s) 13, 179, 778
TaqI TCGA 2 cut(s) 249, 450
TasI AATT 2 cut(s) 207, 663
TfiI GAWTC 1 cut(s) 73
Tru1I TTAA 1 cut(s) 609
Tru9I TTAA 1 cut(s) 609
TscAI CASTG 1 cut(s) 43
TseFI GTSAC 2 cut(s) 226, 542
TseI GCWGC 2 cut(s) 41, 44
Tsp45I GTSAC 2 cut(s) 226, 542
TspDTI ATGAA 3 cut(s) 412, 538, 812
TspRI CASTG 1 cut(s) 43
XapI RAATTY 1 cut(s) 663
XceI RCATGY 1 cut(s) 760
XcmI CCANNNNNNNNNTGG 1 cut(s) 272
XhoI CTCGAG 1 cut(s) 248
XmiI GTMKAC 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.