Rmu_sc0017465.1_g000005

Sphingolipid delta(4)-desaturase DES1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0017465.1
OG0015507
Physical Location & Seq
Forward (+)
20287 .. 21921
1635 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0017465.1_g000005.1.cds

Sequence Viewer

Length: 710 bp
atggggagagggggagatgagagagatggagaagaagaacaagaggagggagtgatggctacggactttttctggtcttatacggatgagcctcacgcttctcggcgccgtcagatcctctctcagtaccctcagattaaggagctttttggtcccgacccattggcttttctcaagattactgtggttgtcttgcttcagctctgcactgctgcagcgcttcacgatgcaggctggctgaagattctggcaatagcctacttcttcggctcttttctcaaccacaacctcttcttggccatccatgagctcagccacaatctcgccttctcaaccccagtctacaaccgttggcttggtatttttgctaacctcccaattggcgttcccatgtcagtcaccttccagaagtatcacctcgagcaccatcgcttccaaggagtagatggcattgacatggacatcccaagcaaagttgaggctcatgtggtgagaaatgtgttcaccaaatccatatgggttctacttcaactcttcttctatgctcttcgacctgtgtttctgaaacctaaaccccctggttgttgggagttcatcaatctttttgtccagatatccctcgatgtcttcatggtttacttttgtggttggaagtcttttgcttatttgattctctctacatttgttgggggtgggatgcaccctatggc

Protein Analysis

237

Amino Acids

27.14

Weight (kDa)

6.18

Isoelectric Point (pI)

47.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015507)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G04930
fragaria_vesca FvH4_3g04930
malus_domestica MD10G1295900.v1.1
prunus_persica Prupe.4G044600_v2.0.a1
pyrus_communis pycom10g24850
rosa_chinensis RchiOBHm_Chr5g0008091
rosa_laevigata RLG00000031535
rosa_multiflora Rmu_sc0017465.1_g000005 Rmu_sc0039673.1_g000001
rosa_roxburghii Rroxscaffold_1G00068390
rosa_rugosa Rorug04G0433300
rosa_samantha Rh5AG061400 Rh5BG058200 Rh5CG068300 Rh5DG057500
rosa_wichuraiana Rw5G005920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 105
AccI GTMKAC 1 cut(s) 342
AclWI GGATC 1 cut(s) 109
AcoI YGGCCR 1 cut(s) 297
AcuI CTGAAG 2 cut(s) 182, 260
AcyI GRCGYC 1 cut(s) 106
AfaI GTAC 1 cut(s) 128
AfeI AGCGCT 1 cut(s) 219
AfiI CCNNNNNNNGG 1 cut(s) 295
AgsI TTSAA 1 cut(s) 530
AjnI CCWGG 1 cut(s) 577
AluBI AGCT 3 cut(s) 145, 202, 310
AluI AGCT 3 cut(s) 145, 202, 310
Alw21I GWGCWC 2 cut(s) 312, 426
AlwI GGATC 1 cut(s) 109
Ama87I CYCGRG 1 cut(s) 419
Aor51HI AGCGCT 1 cut(s) 219
AoxI GGCC 1 cut(s) 297
ApeKI GCWGC 2 cut(s) 212, 215
AspLEI GCGC 2 cut(s) 108, 220
AspS9I GGNCC 1 cut(s) 152
AsuHPI GGTGA 4 cut(s) 391, 407, 496, 502
AvaI CYCGRG 1 cut(s) 419
AvaII GGWCC 1 cut(s) 152
BalI TGGCCA 1 cut(s) 299
BanI GGYRCC 1 cut(s) 105
BanII GRGCYC 1 cut(s) 312
BbsI GAAGAC 1 cut(s) 619
Bbv12I GWGCWC 2 cut(s) 312, 426
BbvI GCAGC 2 cut(s) 199, 227
BccI CCATC 5 cut(s) 20, 49, 308, 435, 440
BceAI ACGGC 1 cut(s) 93
BciT130I CCWGG 1 cut(s) 579
BfmI CTRYAG 1 cut(s) 213
BfoI RGCGCY 2 cut(s) 109, 221
BisI GCNGC 2 cut(s) 213, 216
BlpI GCTNAGC 1 cut(s) 311
BlsI GCNGC 2 cut(s) 214, 217
Bme1390I CCNGG 1 cut(s) 579
Bme18I GGWCC 1 cut(s) 152
BmeT110I CYCGRG 1 cut(s) 419
BmgT120I GGNCC 1 cut(s) 152
BmiI GGNNCC 2 cut(s) 107, 154
BmrFI CCNGG 1 cut(s) 579
BmrI ACTGGG 1 cut(s) 332
BmsI GCATC 2 cut(s) 217, 687
BmuI ACTGGG 1 cut(s) 332
BpiI GAAGAC 1 cut(s) 619
Bpu1102I GCTNAGC 1 cut(s) 311
BpuEI CTTGAG 1 cut(s) 158
BsaHI GRCGYC 1 cut(s) 106
BsaJI CCNNGG 2 cut(s) 436, 577
Bsc4I CCNNNNNNNGG 1 cut(s) 295
Bse1I ACTGG 1 cut(s) 338
BseBI CCWGG 1 cut(s) 579
BseDI CCNNGG 2 cut(s) 436, 577
BseGI GGATG 4 cut(s) 91, 300, 462, 702
BseLI CCNNNNNNNGG 1 cut(s) 295
BseMII CTCAG 3 cut(s) 137, 146, 325
BseNI ACTGG 1 cut(s) 338
BseRI GAGGAG 1 cut(s) 59
BseXI GCAGC 2 cut(s) 199, 227
BsgI GTGCAG 1 cut(s) 190
BshFI GGCC 1 cut(s) 299
BshNI GGYRCC 1 cut(s) 105
BsiHKAI GWGCWC 2 cut(s) 312, 426
BsiHKCI CYCGRG 1 cut(s) 419
BslFI GGGAC 1 cut(s) 138
BslI CCNNNNNNNGG 1 cut(s) 295
BsmFI GGGAC 1 cut(s) 138
BsnI GGCC 1 cut(s) 299
BsoBI CYCGRG 1 cut(s) 419
Bsp1286I GDGCHC 2 cut(s) 312, 426
Bsp143I GATC 1 cut(s) 114
Bsp1720I GCTNAGC 1 cut(s) 311
BspANI GGCC 1 cut(s) 299
BspCNI CTCAG 3 cut(s) 136, 145, 324
BspLI GGNNCC 2 cut(s) 107, 154
BspMAI CTGCAG 1 cut(s) 217
BspPI GGATC 1 cut(s) 109
BspQI GCTCTTC 1 cut(s) 552
BspT107I GGYRCC 1 cut(s) 105
BsrI ACTGG 1 cut(s) 338
BssECI CCNNGG 2 cut(s) 436, 577
BssMI GATC 1 cut(s) 114
BssNI GRCGYC 1 cut(s) 106
BssT1I CCWWGG 1 cut(s) 436
Bst2UI CCWGG 1 cut(s) 579
Bst4CI ACNGT 2 cut(s) 184, 350
Bst6I CTCTTC 3 cut(s) 296, 539, 552
BstACI GRCGYC 1 cut(s) 106
BstC8I GCNNGC 2 cut(s) 232, 236
BstDEI CTNAG 3 cut(s) 123, 132, 311
BstF5I GGATG 4 cut(s) 91, 300, 462, 702
BstH2I RGCGCY 2 cut(s) 109, 221
BstHHI GCGC 2 cut(s) 108, 220
BstKTI GATC 1 cut(s) 117
BstMBI GATC 1 cut(s) 114
BstNI CCWGG 1 cut(s) 579
BstSCI CCNGG 1 cut(s) 577
BstSFI CTRYAG 1 cut(s) 213
BstV1I GCAGC 2 cut(s) 199, 227
BstV2I GAAGAC 1 cut(s) 619
BstX2I RGATCY 1 cut(s) 114
BstYI RGATCY 1 cut(s) 114
BsuRI GGCC 1 cut(s) 299
BtgZI GCGATG 1 cut(s) 413
BtsCI GGATG 4 cut(s) 91, 300, 462, 702
BtsI GCAGTG 1 cut(s) 207
BtsIMutI CAGTG 1 cut(s) 207
Cac8I GCNNGC 2 cut(s) 232, 236
CfoI GCGC 2 cut(s) 108, 220
Cfr13I GGNCC 1 cut(s) 152
Csp6I GTAC 1 cut(s) 127
CviAII CATG 5 cut(s) 305, 391, 457, 485, 631
CviQI GTAC 1 cut(s) 127
DdeI CTNAG 3 cut(s) 123, 132, 311
DinI GGCGCC 1 cut(s) 107
DpnI GATC 1 cut(s) 116
DpnII GATC 1 cut(s) 114
EaeI YGGCCR 1 cut(s) 297
Eam1104I CTCTTC 3 cut(s) 296, 539, 552
EarI CTCTTC 3 cut(s) 296, 539, 552
Ecl136II GAGCTC 1 cut(s) 310
Eco130I CCWWGG 1 cut(s) 436
Eco24I GRGCYC 1 cut(s) 312
Eco32I GATATC 1 cut(s) 615
Eco47I GGWCC 1 cut(s) 152
Eco47III AGCGCT 1 cut(s) 219
Eco53kI GAGCTC 1 cut(s) 310
Eco57I CTGAAG 2 cut(s) 182, 260
Eco88I CYCGRG 1 cut(s) 419
EcoICRI GAGCTC 1 cut(s) 310
EcoRII CCWGG 1 cut(s) 577
EcoRV GATATC 1 cut(s) 615
EcoT14I CCWWGG 1 cut(s) 436
EcoT38I GRGCYC 1 cut(s) 312
EgeI GGCGCC 1 cut(s) 107
EheI GGCGCC 1 cut(s) 107
ErhI CCWWGG 1 cut(s) 436
FaeI CATG 5 cut(s) 308, 394, 460, 488, 634
FaqI GGGAC 1 cut(s) 138
FatI CATG 5 cut(s) 304, 390, 456, 484, 630
FauNDI CATATG 1 cut(s) 515
FblI GTMKAC 1 cut(s) 342
Fnu4HI GCNGC 2 cut(s) 213, 216
FokI GGATG 3 cut(s) 98, 287, 449
FriOI GRGCYC 1 cut(s) 312
Fsp4HI GCNGC 2 cut(s) 213, 216
GlaI GCGC 2 cut(s) 107, 219
GluI GCNGC 2 cut(s) 213, 216
HaeII RGCGCY 2 cut(s) 109, 221
HaeIII GGCC 1 cut(s) 299
HhaI GCGC 2 cut(s) 108, 220
Hin1I GRCGYC 1 cut(s) 106
Hin1II CATG 5 cut(s) 308, 394, 460, 488, 634
Hin6I GCGC 2 cut(s) 106, 218
HinP1I GCGC 2 cut(s) 106, 218
HinfI GANTC 2 cut(s) 244, 670
HphI GGTGA 4 cut(s) 391, 407, 496, 502
Hpy166II GTNNAC 3 cut(s) 343, 504, 637
Hpy188I TCNGA 3 cut(s) 114, 135, 564
Hpy188III TCNNGA 5 cut(s) 155, 175, 224, 406, 610
Hpy8I GTNNAC 3 cut(s) 343, 504, 637
HpyAV CCTTC 2 cut(s) 337, 412
HpyCH4III ACNGT 2 cut(s) 184, 350
HpyCH4V TGCA 4 cut(s) 207, 215, 230, 700
HpyF3I CTNAG 3 cut(s) 123, 132, 311
Hsp92I GRCGYC 1 cut(s) 106
Hsp92II CATG 5 cut(s) 308, 394, 460, 488, 634
HspAI GCGC 2 cut(s) 106, 218
KasI GGCGCC 1 cut(s) 105
Kzo9I GATC 1 cut(s) 114
LguI GCTCTTC 1 cut(s) 552
LmnI GCTCC 1 cut(s) 142
Lsp1109I GCAGC 2 cut(s) 199, 227
LweI GCATC 2 cut(s) 217, 687
MaeIII GTNAC 1 cut(s) 397
MalI GATC 1 cut(s) 116
MboI GATC 1 cut(s) 114
MboII GAAGA 9 cut(s) 44, 47, 253, 256, 283, 526, 529, 539, 619
MfeI CAATTG 1 cut(s) 378
MflI RGATCY 1 cut(s) 114
MhlI GDGCHC 2 cut(s) 312, 426
MlsI TGGCCA 1 cut(s) 299
MluCI AATT 1 cut(s) 378
MluNI TGGCCA 1 cut(s) 299
Mly113I GGCGCC 1 cut(s) 106
MmeI TCCRAC 1 cut(s) 629
Mox20I TGGCCA 1 cut(s) 299
MscI TGGCCA 1 cut(s) 299
MseI TTAA 1 cut(s) 138
MslI CAYNNNNRTG 1 cut(s) 455
Msp20I TGGCCA 1 cut(s) 299
MspR9I CCNGG 1 cut(s) 579
MunI CAATTG 1 cut(s) 378
MvaI CCWGG 1 cut(s) 579
NarI GGCGCC 1 cut(s) 106
NdeI CATATG 1 cut(s) 515
NdeII GATC 1 cut(s) 114
NlaIII CATG 5 cut(s) 308, 394, 460, 488, 634
NlaIV GGNNCC 2 cut(s) 107, 154
NmeAIII GCCGAG 1 cut(s) 82
NmuCI GTSAC 1 cut(s) 397
PaeR7I CTCGAG 1 cut(s) 419
PciSI GCTCTTC 1 cut(s) 552
PfeI GAWTC 2 cut(s) 244, 670
PkrI GCNGC 2 cut(s) 214, 217
PluTI GGCGCC 1 cut(s) 109
Psp124BI GAGCTC 1 cut(s) 312
Psp6I CCWGG 1 cut(s) 577
PspGI CCWGG 1 cut(s) 577
PspN4I GGNNCC 2 cut(s) 107, 154
PspPI GGNCC 1 cut(s) 152
PspXI VCTCGAGB 1 cut(s) 419
PstI CTGCAG 1 cut(s) 217
PsuI RGATCY 1 cut(s) 114
RsaI GTAC 1 cut(s) 128
RsaNI GTAC 1 cut(s) 127
RseI CAYNNNNRTG 1 cut(s) 455
SacI GAGCTC 1 cut(s) 312
SapI GCTCTTC 1 cut(s) 552
SaqAI TTAA 1 cut(s) 138
SatI GCNGC 2 cut(s) 213, 216
Sau3AI GATC 1 cut(s) 114
Sau96I GGNCC 1 cut(s) 152
ScrFI CCNGG 1 cut(s) 579
SduI GDGCHC 2 cut(s) 312, 426
SetI ASST 9 cut(s) 147, 204, 291, 312, 375, 404, 420, 556, 571
SfaNI GCATC 2 cut(s) 217, 687
SfcI CTRYAG 1 cut(s) 213
SfoI GGCGCC 1 cut(s) 107
Sfr274I CTCGAG 1 cut(s) 419
SinI GGWCC 1 cut(s) 152
SlaI CTCGAG 1 cut(s) 419
SmiMI CAYNNNNRTG 1 cut(s) 455
SmlI CTYRAG 2 cut(s) 173, 419
SmoI CTYRAG 2 cut(s) 173, 419
Sse9I AATT 1 cut(s) 378
SspDI GGCGCC 1 cut(s) 105
SstI GAGCTC 1 cut(s) 312
StyD4I CCNGG 1 cut(s) 577
StyI CCWWGG 1 cut(s) 436
TaaI ACNGT 2 cut(s) 184, 350
TaqI TCGA 3 cut(s) 420, 550, 621
TasI AATT 1 cut(s) 378
TfiI GAWTC 2 cut(s) 244, 670
Tru1I TTAA 1 cut(s) 138
Tru9I TTAA 1 cut(s) 138
TscAI CASTG 1 cut(s) 214
TseFI GTSAC 1 cut(s) 397
TseI GCWGC 2 cut(s) 212, 215
Tsp45I GTSAC 1 cut(s) 397
TspDTI ATGAA 2 cut(s) 583, 619
TspGWI ACGGA 2 cut(s) 77, 98
TspRI CASTG 1 cut(s) 214
VpaK11BI GGWCC 1 cut(s) 152
XcmI CCANNNNNNNNNTGG 1 cut(s) 443
XhoI CTCGAG 1 cut(s) 419
XmiI GTMKAC 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.