RchiOBHm_Chr5g0008091

Sphingolipid delta(4)-desaturase DES1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0015507
Physical Location & Seq
Forward (+)
5196708 .. 5198975
2268 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28909

Sequence Viewer

Length: 987 bp
ATGGGGAGAGGGGGAGATGGAGAAGAAGAACAAGAGGAGGGAGTGATGGCTACGGACTTCTTCTGGTCTTATACGGATGAGCCTCACGCTTCTCGGCGCCGTCAGATCCTCTCTCAGTACCCTCAGATTAAGGAGCTTTTTGGCCCTGACCCATTGGCGTTTCTCAAGATTACTGTGGCTGTCTTGCTTCAGCTCTGCACTGCTGCAGCGCTTCACAATGCAGGCTGGCTGAAGATTCTGGCAATAGCCTACTTCTTCGGCTCTTTTCTCAACCACAACCTCTTCTTGGCCATCCATGAGCTCAGCCACAATCTCGCCTTCTCAACCCCAGTCTACAACCGTTGGCTTGGTATCTTTGCTAACCTCCCAATTGGAGTTCCCATGTCTGTCACCTTCCAGAAGTATCACCTCGAGCACCATCGCTTCCAAGGAGTAGATGGCATTGACATGGACATCCCAAGCAAAGTTGAGGCTCATGTGGTGAGAAATGTGTTCACCAAATCCATATGGGTTCTACTTCAACTCTTCTTCTACGCTCTTCGGCCTGTGTTTCTGAAACCGAAACCCCCTGGTTGTTGGGAGTTCATCAATCTTTTTGTCCAGATATCCCTCGATGTCTCCATGGTTTACTTTTGTGGTTGGAAGTCTTTTGCTTATTTGATTCTCTCTACATTTGTTGGGGGTGGGATGCACCCTATGGCTGGTCACTTCATTTCTGAGCATTACGTCTTCAATCCTGATCAAGAGACATATTCTTATTATGGCCCTCTTAATCTCATGACTTGGAGTGTAGGGTACCATAACGAGCACCATGACTTTCCCAGAATTCCTGGGAGCAAGCTCCACAAGGTGAAGTCTATTGCACCAGAATACTATGAGGGTTTGGAGTCTTATAAATCATGGAGTCAGGTCATCTACATGTATATAATGGACAGAACAGTCGGGCCATTTAGCCGAATGAAGAGGAAGCCAAGCAAATCGGAATAG

Protein Analysis

328

Amino Acids

37.83

Weight (kDa)

6.97

Isoelectric Point (pI)

46.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipid_DES PF08557 17 - 52 6e-20 Sphingolipid Delta4-desaturase (DES)
FA_desaturase PF00487 75 - 293 5.1e-23 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0015507)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G04930
fragaria_vesca FvH4_3g04930
malus_domestica MD10G1295900.v1.1
prunus_persica Prupe.4G044600_v2.0.a1
pyrus_communis pycom10g24850
rosa_chinensis RchiOBHm_Chr5g0008091
rosa_laevigata RLG00000031535
rosa_multiflora Rmu_sc0017465.1_g000005 Rmu_sc0039673.1_g000001
rosa_roxburghii Rroxscaffold_1G00068390
rosa_rugosa Rorug04G0433300
rosa_samantha Rh5AG061400 Rh5BG058200 Rh5CG068300 Rh5DG057500
rosa_wichuraiana Rw5G005920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 894
AasI GACNNNNNNGTC 1 cut(s) 938
Acc65I GGTACC 1 cut(s) 795
AccB1I GGYRCC 2 cut(s) 96, 795
AccI GTMKAC 1 cut(s) 333
AclWI GGATC 1 cut(s) 100
AcoI YGGCCR 1 cut(s) 288
AcsI RAATTY 1 cut(s) 825
AcuI CTGAAG 2 cut(s) 173, 251
AcyI GRCGYC 1 cut(s) 97
AdeI CACNNNGTG 1 cut(s) 850
AfaI GTAC 2 cut(s) 119, 797
AfeI AGCGCT 1 cut(s) 210
AfiI CCNNNNNNNGG 2 cut(s) 286, 701
AflIII ACRYGT 1 cut(s) 918
AgsI TTSAA 2 cut(s) 521, 733
AjnI CCWGG 2 cut(s) 568, 829
AluBI AGCT 4 cut(s) 136, 193, 301, 841
AluI AGCT 4 cut(s) 136, 193, 301, 841
Alw21I GWGCWC 3 cut(s) 303, 417, 810
Alw26I GTCTC 2 cut(s) 622, 740
AlwI GGATC 1 cut(s) 100
Ama87I CYCGRG 1 cut(s) 410
Aor51HI AGCGCT 1 cut(s) 210
AoxI GGCC 5 cut(s) 142, 288, 542, 763, 944
ApeKI GCWGC 2 cut(s) 203, 206
ApoI RAATTY 1 cut(s) 825
Asp718I GGTACC 1 cut(s) 795
AspLEI GCGC 2 cut(s) 99, 211
AspS9I GGNCC 3 cut(s) 143, 764, 944
AsuHPI GGTGA 5 cut(s) 382, 398, 487, 493, 862
AvaI CYCGRG 1 cut(s) 410
BalI TGGCCA 1 cut(s) 290
BanI GGYRCC 2 cut(s) 96, 795
BanII GRGCYC 1 cut(s) 303
BbsI GAAGAC 1 cut(s) 721
Bbv12I GWGCWC 3 cut(s) 303, 417, 810
BbvI GCAGC 2 cut(s) 190, 218
BccI CCATC 5 cut(s) 11, 40, 299, 426, 431
BceAI ACGGC 1 cut(s) 84
BciT130I CCWGG 2 cut(s) 570, 831
BclI TGATCA 1 cut(s) 739
BcoDI GTCTC 2 cut(s) 622, 740
BfmI CTRYAG 1 cut(s) 204
BfoI RGCGCY 2 cut(s) 100, 212
BisI GCNGC 2 cut(s) 204, 207
BlpI GCTNAGC 1 cut(s) 302
BlsI GCNGC 2 cut(s) 205, 208
Bme1390I CCNGG 2 cut(s) 570, 831
BmeT110I CYCGRG 1 cut(s) 410
BmgT120I GGNCC 3 cut(s) 143, 764, 944
BmiI GGNNCC 2 cut(s) 98, 797
BmrFI CCNGG 2 cut(s) 570, 831
BmrI ACTGGG 1 cut(s) 323
BmsI GCATC 1 cut(s) 678
BmuI ACTGGG 1 cut(s) 323
BpiI GAAGAC 1 cut(s) 721
Bpu1102I GCTNAGC 1 cut(s) 302
BpuEI CTTGAG 1 cut(s) 149
BsaHI GRCGYC 1 cut(s) 97
BsaJI CCNNGG 4 cut(s) 427, 568, 621, 830
Bsc4I CCNNNNNNNGG 2 cut(s) 286, 701
Bse1I ACTGG 1 cut(s) 329
BseBI CCWGG 2 cut(s) 570, 831
BseDI CCNNGG 4 cut(s) 427, 568, 621, 830
BseGI GGATG 4 cut(s) 82, 291, 453, 693
BseLI CCNNNNNNNGG 2 cut(s) 286, 701
BseMII CTCAG 4 cut(s) 128, 137, 316, 708
BseNI ACTGG 1 cut(s) 329
BseRI GAGGAG 1 cut(s) 50
BseXI GCAGC 2 cut(s) 190, 218
BsgI GTGCAG 1 cut(s) 181
BshFI GGCC 5 cut(s) 144, 290, 544, 765, 946
BshNI GGYRCC 2 cut(s) 96, 795
BsiHKAI GWGCWC 3 cut(s) 303, 417, 810
BsiHKCI CYCGRG 1 cut(s) 410
BslI CCNNNNNNNGG 2 cut(s) 286, 701
BsmAI GTCTC 2 cut(s) 622, 740
BsnI GGCC 5 cut(s) 144, 290, 544, 765, 946
BsoBI CYCGRG 1 cut(s) 410
Bsp1286I GDGCHC 3 cut(s) 303, 417, 810
Bsp143I GATC 2 cut(s) 105, 739
Bsp1720I GCTNAGC 1 cut(s) 302
Bsp19I CCATGG 1 cut(s) 621
BspANI GGCC 5 cut(s) 144, 290, 544, 765, 946
BspCNI CTCAG 4 cut(s) 127, 136, 315, 709
BspHI TCATGA 1 cut(s) 777
BspLI GGNNCC 2 cut(s) 98, 797
BspMAI CTGCAG 1 cut(s) 208
BspPI GGATC 1 cut(s) 100
BspQI GCTCTTC 1 cut(s) 543
BspT107I GGYRCC 2 cut(s) 96, 795
BsrI ACTGG 1 cut(s) 329
BssECI CCNNGG 4 cut(s) 427, 568, 621, 830
BssMI GATC 2 cut(s) 105, 739
BssNI GRCGYC 1 cut(s) 97
BssT1I CCWWGG 2 cut(s) 427, 621
Bst2UI CCWGG 2 cut(s) 570, 831
Bst4CI ACNGT 3 cut(s) 175, 341, 940
Bst6I CTCTTC 4 cut(s) 287, 530, 543, 956
BstACI GRCGYC 1 cut(s) 97
BstC8I GCNNGC 3 cut(s) 223, 227, 839
BstDEI CTNAG 4 cut(s) 114, 123, 302, 717
BstDSI CCRYGG 1 cut(s) 621
BstF5I GGATG 4 cut(s) 82, 291, 453, 693
BstH2I RGCGCY 2 cut(s) 100, 212
BstHHI GCGC 2 cut(s) 99, 211
BstKTI GATC 2 cut(s) 108, 742
BstMAI GTCTC 2 cut(s) 622, 740
BstMBI GATC 2 cut(s) 105, 739
BstNI CCWGG 2 cut(s) 570, 831
BstNSI RCATGY 1 cut(s) 922
BstSCI CCNGG 2 cut(s) 568, 829
BstSFI CTRYAG 1 cut(s) 204
BstV1I GCAGC 2 cut(s) 190, 218
BstV2I GAAGAC 1 cut(s) 721
BstX2I RGATCY 1 cut(s) 105
BstYI RGATCY 1 cut(s) 105
BsuRI GGCC 5 cut(s) 144, 290, 544, 765, 946
BtgI CCRYGG 1 cut(s) 621
BtgZI GCGATG 1 cut(s) 404
BtsCI GGATG 4 cut(s) 82, 291, 453, 693
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 1 cut(s) 198
Cac8I GCNNGC 3 cut(s) 223, 227, 839
CciI TCATGA 1 cut(s) 777
CfoI GCGC 2 cut(s) 99, 211
Cfr13I GGNCC 3 cut(s) 143, 764, 944
Csp6I GTAC 2 cut(s) 118, 796
CviAII CATG 9 cut(s) 296, 382, 448, 476, 622, 778, 812, 900, 919
CviQI GTAC 2 cut(s) 118, 796
DdeI CTNAG 4 cut(s) 114, 123, 302, 717
DinI GGCGCC 1 cut(s) 98
DpnI GATC 2 cut(s) 107, 741
DpnII GATC 2 cut(s) 105, 739
DraIII CACNNNGTG 1 cut(s) 850
DrdI GACNNNNNNGTC 1 cut(s) 938
DseDI GACNNNNNNGTC 1 cut(s) 938
EaeI YGGCCR 1 cut(s) 288
Eam1104I CTCTTC 4 cut(s) 287, 530, 543, 956
EarI CTCTTC 4 cut(s) 287, 530, 543, 956
Ecl136II GAGCTC 1 cut(s) 301
Eco130I CCWWGG 2 cut(s) 427, 621
Eco24I GRGCYC 1 cut(s) 303
Eco32I GATATC 1 cut(s) 606
Eco47III AGCGCT 1 cut(s) 210
Eco53kI GAGCTC 1 cut(s) 301
Eco57I CTGAAG 2 cut(s) 173, 251
Eco88I CYCGRG 1 cut(s) 410
EcoICRI GAGCTC 1 cut(s) 301
EcoRI GAATTC 1 cut(s) 825
EcoRII CCWGG 2 cut(s) 568, 829
EcoRV GATATC 1 cut(s) 606
EcoT14I CCWWGG 2 cut(s) 427, 621
EcoT38I GRGCYC 1 cut(s) 303
EgeI GGCGCC 1 cut(s) 98
EheI GGCGCC 1 cut(s) 98
ErhI CCWWGG 2 cut(s) 427, 621
FaeI CATG 9 cut(s) 299, 385, 451, 479, 625, 781, 815, 903, 922
FatI CATG 9 cut(s) 295, 381, 447, 475, 621, 777, 811, 899, 918
FauNDI CATATG 1 cut(s) 506
FbaI TGATCA 1 cut(s) 739
FblI GTMKAC 1 cut(s) 333
Fnu4HI GCNGC 2 cut(s) 204, 207
FokI GGATG 4 cut(s) 89, 278, 440, 700
FriOI GRGCYC 1 cut(s) 303
Fsp4HI GCNGC 2 cut(s) 204, 207
GlaI GCGC 2 cut(s) 98, 210
GluI GCNGC 2 cut(s) 204, 207
HaeII RGCGCY 2 cut(s) 100, 212
HaeIII GGCC 5 cut(s) 144, 290, 544, 765, 946
HhaI GCGC 2 cut(s) 99, 211
Hin1I GRCGYC 1 cut(s) 97
Hin1II CATG 9 cut(s) 299, 385, 451, 479, 625, 781, 815, 903, 922
Hin6I GCGC 2 cut(s) 97, 209
HinP1I GCGC 2 cut(s) 97, 209
HinfI GANTC 4 cut(s) 235, 661, 887, 904
HphI GGTGA 5 cut(s) 382, 398, 487, 493, 862
Hpy166II GTNNAC 3 cut(s) 334, 495, 628
Hpy188I TCNGA 5 cut(s) 105, 126, 555, 718, 982
Hpy188III TCNNGA 6 cut(s) 166, 397, 601, 737, 743, 778
Hpy8I GTNNAC 3 cut(s) 334, 495, 628
HpyAV CCTTC 2 cut(s) 328, 403
HpyCH4III ACNGT 3 cut(s) 175, 341, 940
HpyCH4IV ACGT 1 cut(s) 726
HpyCH4V TGCA 5 cut(s) 198, 206, 221, 691, 863
HpyF3I CTNAG 4 cut(s) 114, 123, 302, 717
HpySE526I ACGT 1 cut(s) 726
Hsp92I GRCGYC 1 cut(s) 97
Hsp92II CATG 9 cut(s) 299, 385, 451, 479, 625, 781, 815, 903, 922
HspAI GCGC 2 cut(s) 97, 209
KasI GGCGCC 1 cut(s) 96
KpnI GGTACC 1 cut(s) 799
Ksp22I TGATCA 1 cut(s) 739
Kzo9I GATC 2 cut(s) 105, 739
LguI GCTCTTC 1 cut(s) 543
LmnI GCTCC 3 cut(s) 133, 834, 846
Lsp1109I GCAGC 2 cut(s) 190, 218
LweI GCATC 1 cut(s) 678
MaeII ACGT 1 cut(s) 726
MaeIII GTNAC 2 cut(s) 388, 704
MalI GATC 2 cut(s) 107, 741
MboI GATC 2 cut(s) 105, 739
MfeI CAATTG 1 cut(s) 369
MflI RGATCY 1 cut(s) 105
MhlI GDGCHC 3 cut(s) 303, 417, 810
MlsI TGGCCA 1 cut(s) 290
MluCI AATT 2 cut(s) 369, 825
MluNI TGGCCA 1 cut(s) 290
Mly113I GGCGCC 1 cut(s) 97
MlyI GAGTC 2 cut(s) 896, 913
MmeI TCCRAC 1 cut(s) 620
Mox20I TGGCCA 1 cut(s) 290
MscI TGGCCA 1 cut(s) 290
MseI TTAA 2 cut(s) 129, 771
MslI CAYNNNNRTG 2 cut(s) 446, 917
Msp20I TGGCCA 1 cut(s) 290
MspR9I CCNGG 2 cut(s) 570, 831
MunI CAATTG 1 cut(s) 369
MvaI CCWGG 2 cut(s) 570, 831
NarI GGCGCC 1 cut(s) 97
NcoI CCATGG 1 cut(s) 621
NdeI CATATG 1 cut(s) 506
NdeII GATC 2 cut(s) 105, 739
NlaIII CATG 9 cut(s) 299, 385, 451, 479, 625, 781, 815, 903, 922
NlaIV GGNNCC 2 cut(s) 98, 797
NmeAIII GCCGAG 1 cut(s) 73
NmuCI GTSAC 2 cut(s) 388, 704
NspI RCATGY 1 cut(s) 922
PaeR7I CTCGAG 1 cut(s) 410
PagI TCATGA 1 cut(s) 777
PciI ACATGT 1 cut(s) 918
PciSI GCTCTTC 1 cut(s) 543
PfeI GAWTC 2 cut(s) 235, 661
PkrI GCNGC 2 cut(s) 205, 208
PleI GAGTC 2 cut(s) 895, 912
PluTI GGCGCC 1 cut(s) 100
PpsI GAGTC 2 cut(s) 895, 912
PscI ACATGT 1 cut(s) 918
PsiI TTATAA 1 cut(s) 894
Psp124BI GAGCTC 1 cut(s) 303
Psp6I CCWGG 2 cut(s) 568, 829
PspGI CCWGG 2 cut(s) 568, 829
PspN4I GGNNCC 2 cut(s) 98, 797
PspPI GGNCC 3 cut(s) 143, 764, 944
PspXI VCTCGAGB 1 cut(s) 410
PstI CTGCAG 1 cut(s) 208
PsuI RGATCY 1 cut(s) 105
RsaI GTAC 2 cut(s) 119, 797
RsaNI GTAC 2 cut(s) 118, 796
RseI CAYNNNNRTG 2 cut(s) 446, 917
SacI GAGCTC 1 cut(s) 303
SapI GCTCTTC 1 cut(s) 543
SaqAI TTAA 2 cut(s) 129, 771
SatI GCNGC 2 cut(s) 204, 207
Sau3AI GATC 2 cut(s) 105, 739
Sau96I GGNCC 3 cut(s) 143, 764, 944
SchI GAGTC 2 cut(s) 896, 913
ScrFI CCNGG 2 cut(s) 570, 831
SduI GDGCHC 3 cut(s) 303, 417, 810
SfaNI GCATC 1 cut(s) 678
SfcI CTRYAG 1 cut(s) 204
SfoI GGCGCC 1 cut(s) 98
Sfr274I CTCGAG 1 cut(s) 410
SlaI CTCGAG 1 cut(s) 410
SmiMI CAYNNNNRTG 2 cut(s) 446, 917
SmlI CTYRAG 2 cut(s) 164, 410
SmoI CTYRAG 2 cut(s) 164, 410
Sse9I AATT 2 cut(s) 369, 825
SspDI GGCGCC 1 cut(s) 96
SstI GAGCTC 1 cut(s) 303
StyD4I CCNGG 2 cut(s) 568, 829
StyI CCWWGG 2 cut(s) 427, 621
TaaI ACNGT 3 cut(s) 175, 341, 940
TaiI ACGT 1 cut(s) 729
TaqI TCGA 2 cut(s) 411, 612
TasI AATT 2 cut(s) 369, 825
TfiI GAWTC 2 cut(s) 235, 661
Tru1I TTAA 2 cut(s) 129, 771
Tru9I TTAA 2 cut(s) 129, 771
TscAI CASTG 1 cut(s) 205
TseFI GTSAC 2 cut(s) 388, 704
TseI GCWGC 2 cut(s) 203, 206
Tsp45I GTSAC 2 cut(s) 388, 704
TspDTI ATGAA 3 cut(s) 574, 700, 974
TspGWI ACGGA 2 cut(s) 68, 89
TspRI CASTG 1 cut(s) 205
XapI RAATTY 1 cut(s) 825
XceI RCATGY 1 cut(s) 922
XcmI CCANNNNNNNNNTGG 1 cut(s) 434
XhoI CTCGAG 1 cut(s) 410
XmiI GTMKAC 1 cut(s) 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.