Rh5AG061400

Sphingolipid delta(4)-desaturase DES1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0015507
Physical Location & Seq
Forward (+)
5213491 .. 5216031
2541 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG061400.1

Sequence Viewer

Length: 996 bp
ATGGGGAGAGGGGGAGATGAGAGAGATGGAGAAGAAGAACAAGAGGAGGGAGTGATGGCTACGGACTTCTTCTGGTCTTATACGGATGAGCCTCACGCTTCTCGGCGCCGTCAGATCCTCTCTCAGTACCCTCAGATTAAGGAGCTTTTTGGTCCCGACCCATTGGCGTTTCTCAAGATTACTGTGGTTGTCTTGCTTCAGCTCTGCACTGCTGCAGCGCTTCACGATGCAGGCTGGCTGAAGATTCTGGCAATAGCCTACTTCTTCGGCTCTTTTCTCAACCACAACCTCTTCTTGGCCATCCATGAGCTCAGCCACAATCTCGCCTTCTCAACCCCAGTCTACAACCGTTGGCTTGGTATTTTTGCTAACCTCCCAATTGGCGTTCCCATGTCTGTCACCTTCCAGAAGTATCACCTCGAGCACCATCGCTTCCAAGGAGTAGATGGCATTGACATGGACATCCCAAGCAAAGTTGAGGCTCATGTGGTGAGAAATGTGTTCACCAAATCCATATGGGTTCTACTTCAACTCTTCTTCTACGCTCTTCGGCCTGTGTTTCTGAAACCGAAACCCCCTGGTTGTTGGGAGTTCATCAATCTTTTTGTCCAGATATCCCTCGATGTCTCCATGGTTTACTTTTGTGGTTGGAAGTCTTTTGCTTATTTGATTCTCTCTACATTTGTTGGGGGTGGGATGCACCCTATGGCTGGTCATTTCATTTCTGAGCATTACGTCTTCAATCCTGATCAAGAGACATATTCTTATTATGGCCCTCTTAATCTCATGACTTGGAGTGTAGGGTACCATAACGAGCACCATGACTTTCCCAGAATTCCTGGGAGCAAGCTCCACAAGGTGAAGTCTATTGCACCAGAATACTATGAGGGTTTGGAGTCTTATAAATCATGGAGTCAGGTCATCTACATGTATATAATGGACAGAACAGTCGGGCCATTTAGCCGAATGAAGAGGAAGCCAAGCAAATCGGAATAG

Protein Analysis

331

Amino Acids

38.25

Weight (kDa)

6.6

Isoelectric Point (pI)

45.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipid_DES PF08557 20 - 55 6.1e-20 Sphingolipid Delta4-desaturase (DES)
FA_desaturase PF00487 78 - 296 4.6e-23 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015507)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G04930
fragaria_vesca FvH4_3g04930
malus_domestica MD10G1295900.v1.1
prunus_persica Prupe.4G044600_v2.0.a1
pyrus_communis pycom10g24850
rosa_chinensis RchiOBHm_Chr5g0008091
rosa_laevigata RLG00000031535
rosa_multiflora Rmu_sc0017465.1_g000005 Rmu_sc0039673.1_g000001
rosa_roxburghii Rroxscaffold_1G00068390
rosa_rugosa Rorug04G0433300
rosa_samantha Rh5AG061400 Rh5BG058200 Rh5CG068300 Rh5DG057500
rosa_wichuraiana Rw5G005920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 903
AasI GACNNNNNNGTC 1 cut(s) 947
Acc65I GGTACC 1 cut(s) 804
AccB1I GGYRCC 2 cut(s) 105, 804
AccI GTMKAC 1 cut(s) 342
AclWI GGATC 1 cut(s) 109
AcoI YGGCCR 1 cut(s) 297
AcsI RAATTY 1 cut(s) 834
AcuI CTGAAG 2 cut(s) 182, 260
AcyI GRCGYC 1 cut(s) 106
AdeI CACNNNGTG 1 cut(s) 859
AfaI GTAC 2 cut(s) 128, 806
AfeI AGCGCT 1 cut(s) 219
AfiI CCNNNNNNNGG 2 cut(s) 295, 710
AflIII ACRYGT 1 cut(s) 927
AgsI TTSAA 2 cut(s) 530, 742
AjnI CCWGG 2 cut(s) 577, 838
AluBI AGCT 4 cut(s) 145, 202, 310, 850
AluI AGCT 4 cut(s) 145, 202, 310, 850
Alw21I GWGCWC 3 cut(s) 312, 426, 819
Alw26I GTCTC 2 cut(s) 631, 749
AlwI GGATC 1 cut(s) 109
Ama87I CYCGRG 1 cut(s) 419
Aor51HI AGCGCT 1 cut(s) 219
AoxI GGCC 4 cut(s) 297, 551, 772, 953
ApeKI GCWGC 2 cut(s) 212, 215
ApoI RAATTY 1 cut(s) 834
Asp718I GGTACC 1 cut(s) 804
AspLEI GCGC 2 cut(s) 108, 220
AspS9I GGNCC 3 cut(s) 152, 773, 953
AsuHPI GGTGA 5 cut(s) 391, 407, 496, 502, 871
AvaI CYCGRG 1 cut(s) 419
AvaII GGWCC 1 cut(s) 152
BalI TGGCCA 1 cut(s) 299
BanI GGYRCC 2 cut(s) 105, 804
BanII GRGCYC 1 cut(s) 312
BbsI GAAGAC 1 cut(s) 730
Bbv12I GWGCWC 3 cut(s) 312, 426, 819
BbvI GCAGC 2 cut(s) 199, 227
BccI CCATC 5 cut(s) 20, 49, 308, 435, 440
BceAI ACGGC 1 cut(s) 93
BciT130I CCWGG 2 cut(s) 579, 840
BclI TGATCA 1 cut(s) 748
BcoDI GTCTC 2 cut(s) 631, 749
BfmI CTRYAG 1 cut(s) 213
BfoI RGCGCY 2 cut(s) 109, 221
BisI GCNGC 2 cut(s) 213, 216
BlpI GCTNAGC 1 cut(s) 311
BlsI GCNGC 2 cut(s) 214, 217
Bme1390I CCNGG 2 cut(s) 579, 840
Bme18I GGWCC 1 cut(s) 152
BmeT110I CYCGRG 1 cut(s) 419
BmgT120I GGNCC 3 cut(s) 152, 773, 953
BmiI GGNNCC 3 cut(s) 107, 154, 806
BmrFI CCNGG 2 cut(s) 579, 840
BmrI ACTGGG 1 cut(s) 332
BmsI GCATC 2 cut(s) 217, 687
BmuI ACTGGG 1 cut(s) 332
BpiI GAAGAC 1 cut(s) 730
Bpu1102I GCTNAGC 1 cut(s) 311
BpuEI CTTGAG 1 cut(s) 158
BsaHI GRCGYC 1 cut(s) 106
BsaJI CCNNGG 4 cut(s) 436, 577, 630, 839
Bsc4I CCNNNNNNNGG 2 cut(s) 295, 710
Bse1I ACTGG 1 cut(s) 338
BseBI CCWGG 2 cut(s) 579, 840
BseDI CCNNGG 4 cut(s) 436, 577, 630, 839
BseGI GGATG 4 cut(s) 91, 300, 462, 702
BseLI CCNNNNNNNGG 2 cut(s) 295, 710
BseMII CTCAG 4 cut(s) 137, 146, 325, 717
BseNI ACTGG 1 cut(s) 338
BseRI GAGGAG 1 cut(s) 59
BseXI GCAGC 2 cut(s) 199, 227
BsgI GTGCAG 1 cut(s) 190
BshFI GGCC 4 cut(s) 299, 553, 774, 955
BshNI GGYRCC 2 cut(s) 105, 804
BsiHKAI GWGCWC 3 cut(s) 312, 426, 819
BsiHKCI CYCGRG 1 cut(s) 419
BslFI GGGAC 1 cut(s) 138
BslI CCNNNNNNNGG 2 cut(s) 295, 710
BsmAI GTCTC 2 cut(s) 631, 749
BsmFI GGGAC 1 cut(s) 138
BsnI GGCC 4 cut(s) 299, 553, 774, 955
BsoBI CYCGRG 1 cut(s) 419
Bsp1286I GDGCHC 3 cut(s) 312, 426, 819
Bsp143I GATC 2 cut(s) 114, 748
Bsp1720I GCTNAGC 1 cut(s) 311
Bsp19I CCATGG 1 cut(s) 630
BspANI GGCC 4 cut(s) 299, 553, 774, 955
BspCNI CTCAG 4 cut(s) 136, 145, 324, 718
BspHI TCATGA 1 cut(s) 786
BspLI GGNNCC 3 cut(s) 107, 154, 806
BspMAI CTGCAG 1 cut(s) 217
BspPI GGATC 1 cut(s) 109
BspQI GCTCTTC 1 cut(s) 552
BspT107I GGYRCC 2 cut(s) 105, 804
BsrI ACTGG 1 cut(s) 338
BssECI CCNNGG 4 cut(s) 436, 577, 630, 839
BssMI GATC 2 cut(s) 114, 748
BssNI GRCGYC 1 cut(s) 106
BssT1I CCWWGG 2 cut(s) 436, 630
Bst2UI CCWGG 2 cut(s) 579, 840
Bst4CI ACNGT 3 cut(s) 184, 350, 949
Bst6I CTCTTC 4 cut(s) 296, 539, 552, 965
BstACI GRCGYC 1 cut(s) 106
BstC8I GCNNGC 3 cut(s) 232, 236, 848
BstDEI CTNAG 4 cut(s) 123, 132, 311, 726
BstDSI CCRYGG 1 cut(s) 630
BstF5I GGATG 4 cut(s) 91, 300, 462, 702
BstH2I RGCGCY 2 cut(s) 109, 221
BstHHI GCGC 2 cut(s) 108, 220
BstKTI GATC 2 cut(s) 117, 751
BstMAI GTCTC 2 cut(s) 631, 749
BstMBI GATC 2 cut(s) 114, 748
BstNI CCWGG 2 cut(s) 579, 840
BstNSI RCATGY 1 cut(s) 931
BstSCI CCNGG 2 cut(s) 577, 838
BstSFI CTRYAG 1 cut(s) 213
BstV1I GCAGC 2 cut(s) 199, 227
BstV2I GAAGAC 1 cut(s) 730
BstX2I RGATCY 1 cut(s) 114
BstYI RGATCY 1 cut(s) 114
BsuRI GGCC 4 cut(s) 299, 553, 774, 955
BtgI CCRYGG 1 cut(s) 630
BtgZI GCGATG 1 cut(s) 413
BtsCI GGATG 4 cut(s) 91, 300, 462, 702
BtsI GCAGTG 1 cut(s) 207
BtsIMutI CAGTG 1 cut(s) 207
Cac8I GCNNGC 3 cut(s) 232, 236, 848
CciI TCATGA 1 cut(s) 786
CfoI GCGC 2 cut(s) 108, 220
Cfr13I GGNCC 3 cut(s) 152, 773, 953
Csp6I GTAC 2 cut(s) 127, 805
CviAII CATG 9 cut(s) 305, 391, 457, 485, 631, 787, 821, 909, 928
CviQI GTAC 2 cut(s) 127, 805
DdeI CTNAG 4 cut(s) 123, 132, 311, 726
DinI GGCGCC 1 cut(s) 107
DpnI GATC 2 cut(s) 116, 750
DpnII GATC 2 cut(s) 114, 748
DraIII CACNNNGTG 1 cut(s) 859
DrdI GACNNNNNNGTC 1 cut(s) 947
DseDI GACNNNNNNGTC 1 cut(s) 947
EaeI YGGCCR 1 cut(s) 297
Eam1104I CTCTTC 4 cut(s) 296, 539, 552, 965
EarI CTCTTC 4 cut(s) 296, 539, 552, 965
Ecl136II GAGCTC 1 cut(s) 310
Eco130I CCWWGG 2 cut(s) 436, 630
Eco24I GRGCYC 1 cut(s) 312
Eco32I GATATC 1 cut(s) 615
Eco47I GGWCC 1 cut(s) 152
Eco47III AGCGCT 1 cut(s) 219
Eco53kI GAGCTC 1 cut(s) 310
Eco57I CTGAAG 2 cut(s) 182, 260
Eco88I CYCGRG 1 cut(s) 419
EcoICRI GAGCTC 1 cut(s) 310
EcoRI GAATTC 1 cut(s) 834
EcoRII CCWGG 2 cut(s) 577, 838
EcoRV GATATC 1 cut(s) 615
EcoT14I CCWWGG 2 cut(s) 436, 630
EcoT38I GRGCYC 1 cut(s) 312
EgeI GGCGCC 1 cut(s) 107
EheI GGCGCC 1 cut(s) 107
ErhI CCWWGG 2 cut(s) 436, 630
FaeI CATG 9 cut(s) 308, 394, 460, 488, 634, 790, 824, 912, 931
FaqI GGGAC 1 cut(s) 138
FatI CATG 9 cut(s) 304, 390, 456, 484, 630, 786, 820, 908, 927
FauNDI CATATG 1 cut(s) 515
FbaI TGATCA 1 cut(s) 748
FblI GTMKAC 1 cut(s) 342
Fnu4HI GCNGC 2 cut(s) 213, 216
FokI GGATG 4 cut(s) 98, 287, 449, 709
FriOI GRGCYC 1 cut(s) 312
Fsp4HI GCNGC 2 cut(s) 213, 216
GlaI GCGC 2 cut(s) 107, 219
GluI GCNGC 2 cut(s) 213, 216
HaeII RGCGCY 2 cut(s) 109, 221
HaeIII GGCC 4 cut(s) 299, 553, 774, 955
HhaI GCGC 2 cut(s) 108, 220
Hin1I GRCGYC 1 cut(s) 106
Hin1II CATG 9 cut(s) 308, 394, 460, 488, 634, 790, 824, 912, 931
Hin6I GCGC 2 cut(s) 106, 218
HinP1I GCGC 2 cut(s) 106, 218
HinfI GANTC 4 cut(s) 244, 670, 896, 913
HphI GGTGA 5 cut(s) 391, 407, 496, 502, 871
Hpy166II GTNNAC 3 cut(s) 343, 504, 637
Hpy188I TCNGA 5 cut(s) 114, 135, 564, 727, 991
Hpy188III TCNNGA 8 cut(s) 155, 175, 224, 406, 610, 746, 752, 787
Hpy8I GTNNAC 3 cut(s) 343, 504, 637
HpyAV CCTTC 2 cut(s) 337, 412
HpyCH4III ACNGT 3 cut(s) 184, 350, 949
HpyCH4IV ACGT 1 cut(s) 735
HpyCH4V TGCA 5 cut(s) 207, 215, 230, 700, 872
HpyF3I CTNAG 4 cut(s) 123, 132, 311, 726
HpySE526I ACGT 1 cut(s) 735
Hsp92I GRCGYC 1 cut(s) 106
Hsp92II CATG 9 cut(s) 308, 394, 460, 488, 634, 790, 824, 912, 931
HspAI GCGC 2 cut(s) 106, 218
KasI GGCGCC 1 cut(s) 105
KpnI GGTACC 1 cut(s) 808
Ksp22I TGATCA 1 cut(s) 748
Kzo9I GATC 2 cut(s) 114, 748
LguI GCTCTTC 1 cut(s) 552
LmnI GCTCC 3 cut(s) 142, 843, 855
Lsp1109I GCAGC 2 cut(s) 199, 227
LweI GCATC 2 cut(s) 217, 687
MaeII ACGT 1 cut(s) 735
MaeIII GTNAC 1 cut(s) 397
MalI GATC 2 cut(s) 116, 750
MboI GATC 2 cut(s) 114, 748
MfeI CAATTG 1 cut(s) 378
MflI RGATCY 1 cut(s) 114
MhlI GDGCHC 3 cut(s) 312, 426, 819
MlsI TGGCCA 1 cut(s) 299
MluCI AATT 2 cut(s) 378, 834
MluNI TGGCCA 1 cut(s) 299
Mly113I GGCGCC 1 cut(s) 106
MlyI GAGTC 2 cut(s) 905, 922
MmeI TCCRAC 1 cut(s) 629
Mox20I TGGCCA 1 cut(s) 299
MscI TGGCCA 1 cut(s) 299
MseI TTAA 2 cut(s) 138, 780
MslI CAYNNNNRTG 2 cut(s) 455, 926
Msp20I TGGCCA 1 cut(s) 299
MspR9I CCNGG 2 cut(s) 579, 840
MunI CAATTG 1 cut(s) 378
MvaI CCWGG 2 cut(s) 579, 840
NarI GGCGCC 1 cut(s) 106
NcoI CCATGG 1 cut(s) 630
NdeI CATATG 1 cut(s) 515
NdeII GATC 2 cut(s) 114, 748
NlaIII CATG 9 cut(s) 308, 394, 460, 488, 634, 790, 824, 912, 931
NlaIV GGNNCC 3 cut(s) 107, 154, 806
NmeAIII GCCGAG 1 cut(s) 82
NmuCI GTSAC 1 cut(s) 397
NspI RCATGY 1 cut(s) 931
PaeR7I CTCGAG 1 cut(s) 419
PagI TCATGA 1 cut(s) 786
PciI ACATGT 1 cut(s) 927
PciSI GCTCTTC 1 cut(s) 552
PfeI GAWTC 2 cut(s) 244, 670
PkrI GCNGC 2 cut(s) 214, 217
PleI GAGTC 2 cut(s) 904, 921
PluTI GGCGCC 1 cut(s) 109
PpsI GAGTC 2 cut(s) 904, 921
PscI ACATGT 1 cut(s) 927
PsiI TTATAA 1 cut(s) 903
Psp124BI GAGCTC 1 cut(s) 312
Psp6I CCWGG 2 cut(s) 577, 838
PspGI CCWGG 2 cut(s) 577, 838
PspN4I GGNNCC 3 cut(s) 107, 154, 806
PspPI GGNCC 3 cut(s) 152, 773, 953
PspXI VCTCGAGB 1 cut(s) 419
PstI CTGCAG 1 cut(s) 217
PsuI RGATCY 1 cut(s) 114
RsaI GTAC 2 cut(s) 128, 806
RsaNI GTAC 2 cut(s) 127, 805
RseI CAYNNNNRTG 2 cut(s) 455, 926
SacI GAGCTC 1 cut(s) 312
SapI GCTCTTC 1 cut(s) 552
SaqAI TTAA 2 cut(s) 138, 780
SatI GCNGC 2 cut(s) 213, 216
Sau3AI GATC 2 cut(s) 114, 748
Sau96I GGNCC 3 cut(s) 152, 773, 953
SchI GAGTC 2 cut(s) 905, 922
ScrFI CCNGG 2 cut(s) 579, 840
SduI GDGCHC 3 cut(s) 312, 426, 819
SfaNI GCATC 2 cut(s) 217, 687
SfcI CTRYAG 1 cut(s) 213
SfoI GGCGCC 1 cut(s) 107
Sfr274I CTCGAG 1 cut(s) 419
SinI GGWCC 1 cut(s) 152
SlaI CTCGAG 1 cut(s) 419
SmiMI CAYNNNNRTG 2 cut(s) 455, 926
SmlI CTYRAG 2 cut(s) 173, 419
SmoI CTYRAG 2 cut(s) 173, 419
Sse9I AATT 2 cut(s) 378, 834
SspDI GGCGCC 1 cut(s) 105
SstI GAGCTC 1 cut(s) 312
StyD4I CCNGG 2 cut(s) 577, 838
StyI CCWWGG 2 cut(s) 436, 630
TaaI ACNGT 3 cut(s) 184, 350, 949
TaiI ACGT 1 cut(s) 738
TaqI TCGA 2 cut(s) 420, 621
TasI AATT 2 cut(s) 378, 834
TfiI GAWTC 2 cut(s) 244, 670
Tru1I TTAA 2 cut(s) 138, 780
Tru9I TTAA 2 cut(s) 138, 780
TscAI CASTG 1 cut(s) 214
TseFI GTSAC 1 cut(s) 397
TseI GCWGC 2 cut(s) 212, 215
Tsp45I GTSAC 1 cut(s) 397
TspDTI ATGAA 3 cut(s) 583, 709, 983
TspGWI ACGGA 2 cut(s) 77, 98
TspRI CASTG 1 cut(s) 214
VpaK11BI GGWCC 1 cut(s) 152
XapI RAATTY 1 cut(s) 834
XceI RCATGY 1 cut(s) 931
XcmI CCANNNNNNNNNTGG 1 cut(s) 443
XhoI CTCGAG 1 cut(s) 419
XmiI GTMKAC 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.