MD10G1320900.v1.1

Senescence regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0013122
Physical Location & Seq
Forward (+)
40296682 .. 40297299
618 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1320900.v1.1.491

Sequence Viewer

Length: 618 bp
ATGGCGAAGGGCCGGAAACTGACGCAGAGCCGCAGCGAACGGTTGCTCGGGACCTACAGCGGCTACAGCCACGCCCATGACTCGCCGGAGCTCGGCGAGGACGATGTCTGGTCGATGGTCAACAGCGTCGACGAGAGGGAGGACCACGAAACCGAGAACTCGCAGGGCGAATGGAGCCCACACGCCTTCCCGGAGAGCACCGTTGGTATTCAGGCGAGGAAGGGCCGCCATATTGCCAGAGACGACCGCAACGTGGGCGGGCTGTCATTGGCCTTTGACGATTCTGGCAAGACGGCGTCGTCGCCTCGGATCGTGCACCAGTTCCGCGGACACGACAGCGTGGCGTCCCCACGTGGGCACCACATGGCCACGTCAGCTCCGGTGAACGTGCCTGACTGGAGCAAAATTCTCCGGGTCGACTCGGTGGACTCGCTGTATGAAATGGGCGACGGGTTAGATGGCAATGACTCGGAGATGATTCCCCCACACGAGTACCTGGCGCGTGAGCACACGCGCGGCGCGACGTCGGTTTTCGAAGGCGTAGGGCGAACCTTGAAGGGCCGGGACATGAGGCGGGTCAGGGATGCAGTGTGGAGCCGGACCGGGTTTGATGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Senescence_reg

Protein Analysis

206

Amino Acids

22.72

Weight (kDa)

5.86

Isoelectric Point (pI)

39.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Senescence_reg PF04520 30 - 203 4e-42 Senescence regulator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013122)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11700 AT1G61930
fragaria_vesca FvH4_3g02140
malus_domestica MD05G1345500.v1.1 MD10G1320900.v1.1
prunus_persica Prupe.4G021400_v2.0.a1
pyrus_communis pycom10g27160
rosa_chinensis RchiOBHm_Chr5g0003211
rosa_laevigata RLG00000031087
rosa_multiflora Rmu_sc0000146.1_g000038
rosa_roxburghii Rroxscaffold_1G00072790
rosa_rugosa Rorug04G0401400
rosa_samantha Rh5AG026500 Rh5BG027700 Rh5CG029300 Rh5DG026000
rosa_wichuraiana Rw0G023550 Rw5G002440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 298
AatII GACGTC 1 cut(s) 527
AccB1I GGYRCC 1 cut(s) 357
AccI GTMKAC 2 cut(s) 129, 417
AccII CGCG 5 cut(s) 327, 502, 514, 516, 521
AciI CCGC 9 cut(s) 31, 60, 226, 247, 258, 325, 327, 516, 574
AclWI GGATC 1 cut(s) 317
AcoI YGGCCR 1 cut(s) 366
AcsI RAATTY 1 cut(s) 405
AcvI CACGTG 1 cut(s) 353
AcyI GRCGYC 3 cut(s) 296, 344, 524
AfaI GTAC 1 cut(s) 494
AfiI CCNNNNNNNGG 3 cut(s) 92, 253, 354
AgsI TTSAA 1 cut(s) 556
AjiI CACGTC 1 cut(s) 372
AjnI CCWGG 1 cut(s) 495
AluBI AGCT 2 cut(s) 91, 377
AluI AGCT 2 cut(s) 91, 377
Alw21I GWGCWC 4 cut(s) 93, 200, 318, 510
Alw26I GTCTC 1 cut(s) 234
Alw44I GTGCAC 1 cut(s) 314
AlwI GGATC 1 cut(s) 317
Ama87I CYCGRG 1 cut(s) 47
AoxI GGCC 5 cut(s) 10, 223, 270, 366, 559
ApaLI GTGCAC 1 cut(s) 314
ApeKI GCWGC 1 cut(s) 33
ApoI RAATTY 1 cut(s) 405
AspLEI GCGC 3 cut(s) 502, 516, 521
AspS9I GGNCC 6 cut(s) 10, 51, 142, 223, 559, 600
AsuC2I CCSGG 4 cut(s) 191, 413, 563, 604
AsuHPI GGTGA 1 cut(s) 394
AsuII TTCGAA 1 cut(s) 534
AvaI CYCGRG 1 cut(s) 47
AvaII GGWCC 3 cut(s) 51, 142, 600
BaeGI GKGCMC 2 cut(s) 318, 360
BaeI ACNNNNGTAYC 2 cut(s) 476, 509
BalI TGGCCA 1 cut(s) 368
BanI GGYRCC 1 cut(s) 357
BanII GRGCYC 2 cut(s) 93, 179
BauI CACGAG 1 cut(s) 488
BbrPI CACGTG 1 cut(s) 353
Bbv12I GWGCWC 4 cut(s) 93, 200, 318, 510
BbvI GCAGC 1 cut(s) 45
BccI CCATC 3 cut(s) 109, 452, 605
BceAI ACGGC 1 cut(s) 309
BciT130I CCWGG 1 cut(s) 497
BcnI CCSGG 4 cut(s) 191, 413, 563, 604
BcoDI GTCTC 1 cut(s) 234
BfmI CTRYAG 2 cut(s) 55, 64
BisI GCNGC 5 cut(s) 31, 34, 61, 226, 517
BlsI GCNGC 5 cut(s) 32, 35, 62, 227, 518
Bme1390I CCNGG 5 cut(s) 191, 413, 497, 563, 604
Bme18I GGWCC 3 cut(s) 51, 142, 600
BmeT110I CYCGRG 1 cut(s) 47
BmgBI CACGTC 1 cut(s) 372
BmgT120I GGNCC 6 cut(s) 10, 51, 142, 223, 559, 600
BmiI GGNNCC 4 cut(s) 52, 176, 359, 596
BmrFI CCNGG 5 cut(s) 191, 413, 497, 563, 604
BmsI GCATC 1 cut(s) 574
BpmI CTGGAG 1 cut(s) 418
Bpu14I TTCGAA 1 cut(s) 534
BpuMI CCSGG 4 cut(s) 191, 413, 563, 604
BsaAI YACGTR 1 cut(s) 353
BsaHI GRCGYC 3 cut(s) 296, 344, 524
BsaJI CCNNGG 2 cut(s) 305, 325
BsaWI WCCGGW 1 cut(s) 379
BsaXI ACNNNNNCTCC 2 cut(s) 361, 391
Bsc4I CCNNNNNNNGG 3 cut(s) 92, 253, 354
Bse1I ACTGG 2 cut(s) 319, 401
Bse3DI GCAATG 1 cut(s) 469
BseBI CCWGG 1 cut(s) 497
BseDI CCNNGG 2 cut(s) 305, 325
BseGI GGATG 1 cut(s) 589
BseLI CCNNNNNNNGG 3 cut(s) 92, 253, 354
BseMI GCAATG 1 cut(s) 469
BseNI ACTGG 2 cut(s) 319, 401
BseSI GKGCMC 2 cut(s) 318, 360
BseXI GCAGC 1 cut(s) 45
Bsh1236I CGCG 5 cut(s) 327, 502, 514, 516, 521
Bsh1285I CGRYCG 1 cut(s) 247
BshFI GGCC 5 cut(s) 12, 225, 272, 368, 561
BshNI GGYRCC 1 cut(s) 357
BsiEI CGRYCG 1 cut(s) 247
BsiHKAI GWGCWC 4 cut(s) 93, 200, 318, 510
BsiHKCI CYCGRG 1 cut(s) 47
BsiSI CCGG 8 cut(s) 13, 86, 191, 380, 412, 562, 598, 603
BslFI GGGAC 3 cut(s) 64, 331, 578
BslI CCNNNNNNNGG 3 cut(s) 92, 253, 354
BsmAI GTCTC 1 cut(s) 234
BsmBI CGTCTC 1 cut(s) 234
BsmFI GGGAC 3 cut(s) 64, 331, 578
BsnI GGCC 5 cut(s) 12, 225, 272, 368, 561
BsoBI CYCGRG 1 cut(s) 47
Bsp119I TTCGAA 1 cut(s) 534
Bsp1286I GDGCHC 6 cut(s) 93, 179, 200, 318, 360, 510
Bsp143I GATC 1 cut(s) 309
BspACI CCGC 9 cut(s) 31, 60, 226, 247, 258, 325, 327, 516, 574
BspANI GGCC 5 cut(s) 12, 225, 272, 368, 561
BspFNI CGCG 5 cut(s) 327, 502, 514, 516, 521
BspLI GGNNCC 4 cut(s) 52, 176, 359, 596
BspPI GGATC 1 cut(s) 317
BspT104I TTCGAA 1 cut(s) 534
BspT107I GGYRCC 1 cut(s) 357
BsrDI GCAATG 1 cut(s) 469
BsrI ACTGG 2 cut(s) 319, 401
BssECI CCNNGG 2 cut(s) 305, 325
BssMI GATC 1 cut(s) 309
BssNI GRCGYC 3 cut(s) 296, 344, 524
BssSI CACGAG 1 cut(s) 488
Bst2BI CACGAG 1 cut(s) 488
Bst2UI CCWGG 1 cut(s) 497
Bst4CI ACNGT 2 cut(s) 42, 202
BstACI GRCGYC 3 cut(s) 296, 344, 524
BstBAI YACGTR 1 cut(s) 353
BstBI TTCGAA 1 cut(s) 534
BstC8I GCNNGC 1 cut(s) 260
BstDSI CCRYGG 1 cut(s) 325
BstF5I GGATG 1 cut(s) 589
BstFNI CGCG 5 cut(s) 327, 502, 514, 516, 521
BstHHI GCGC 3 cut(s) 502, 516, 521
BstKTI GATC 1 cut(s) 312
BstMAI GTCTC 1 cut(s) 234
BstMBI GATC 1 cut(s) 309
BstMCI CGRYCG 1 cut(s) 247
BstMWI GCNNNNNNNGC 4 cut(s) 66, 174, 255, 374
BstNI CCWGG 1 cut(s) 497
BstSCI CCNGG 5 cut(s) 189, 411, 495, 561, 602
BstSFI CTRYAG 2 cut(s) 55, 64
BstSLI GKGCMC 2 cut(s) 318, 360
BstUI CGCG 5 cut(s) 327, 502, 514, 516, 521
BstV1I GCAGC 1 cut(s) 45
BsuRI GGCC 5 cut(s) 12, 225, 272, 368, 561
BtgI CCRYGG 1 cut(s) 325
BtrI CACGTC 1 cut(s) 372
BtsCI GGATG 1 cut(s) 589
BtsI GCAGTG 1 cut(s) 594
BtsIMutI CAGTG 1 cut(s) 594
Cac8I GCNNGC 1 cut(s) 260
CfoI GCGC 3 cut(s) 502, 516, 521
Cfr13I GGNCC 6 cut(s) 10, 51, 142, 223, 559, 600
Cfr42I CCGCGG 1 cut(s) 328
CpoI CGGWCCG 1 cut(s) 600
CseI GACGC 4 cut(s) 31, 115, 285, 333
Csp6I GTAC 1 cut(s) 493
CspI CGGWCCG 1 cut(s) 600
CviAII CATG 3 cut(s) 77, 364, 568
CviQI GTAC 1 cut(s) 493
DpnI GATC 1 cut(s) 311
DpnII GATC 1 cut(s) 309
DrdI GACNNNNNNGTC 1 cut(s) 298
DseDI GACNNNNNNGTC 1 cut(s) 298
EaeI YGGCCR 1 cut(s) 366
Ecl136II GAGCTC 1 cut(s) 91
Eco24I GRGCYC 2 cut(s) 93, 179
Eco47I GGWCC 3 cut(s) 51, 142, 600
Eco53kI GAGCTC 1 cut(s) 91
Eco72I CACGTG 1 cut(s) 353
Eco88I CYCGRG 1 cut(s) 47
EcoICRI GAGCTC 1 cut(s) 91
EcoO109I RGGNCCY 1 cut(s) 51
EcoRII CCWGG 1 cut(s) 495
EcoT38I GRGCYC 2 cut(s) 93, 179
Esp3I CGTCTC 1 cut(s) 234
FaeI CATG 3 cut(s) 80, 367, 571
FaiI YATR 5 cut(s) 78, 231, 365, 438, 569
FaqI GGGAC 3 cut(s) 64, 331, 578
FatI CATG 3 cut(s) 76, 363, 567
FauI CCCGC 2 cut(s) 251, 567
FblI GTMKAC 2 cut(s) 129, 417
Fnu4HI GCNGC 5 cut(s) 31, 34, 61, 226, 517
FokI GGATG 1 cut(s) 596
FriOI GRGCYC 2 cut(s) 93, 179
Fsp4HI GCNGC 5 cut(s) 31, 34, 61, 226, 517
GlaI GCGC 3 cut(s) 501, 515, 520
GluI GCNGC 5 cut(s) 31, 34, 61, 226, 517
GsuI CTGGAG 1 cut(s) 418
HaeIII GGCC 5 cut(s) 12, 225, 272, 368, 561
HapII CCGG 8 cut(s) 13, 86, 191, 380, 412, 562, 598, 603
HgaI GACGC 4 cut(s) 31, 115, 285, 333
HhaI GCGC 3 cut(s) 502, 516, 521
Hin1I GRCGYC 3 cut(s) 296, 344, 524
Hin1II CATG 3 cut(s) 80, 367, 571
Hin6I GCGC 3 cut(s) 500, 514, 519
HinP1I GCGC 3 cut(s) 500, 514, 519
HincII GTYRAC 3 cut(s) 121, 130, 418
HindII GTYRAC 3 cut(s) 121, 130, 418
HinfI GANTC 6 cut(s) 80, 281, 419, 428, 467, 478
HpaII CCGG 8 cut(s) 13, 86, 191, 380, 412, 562, 598, 603
HphI GGTGA 1 cut(s) 394
Hpy166II GTNNAC 6 cut(s) 121, 130, 316, 385, 418, 427
Hpy188I TCNGA 2 cut(s) 309, 472
Hpy188III TCNNGA 1 cut(s) 49
Hpy8I GTNNAC 6 cut(s) 121, 130, 316, 385, 418, 427
Hpy99I CGWCG 7 cut(s) 131, 134, 301, 304, 452, 526, 529
HpyAV CCTTC 4 cut(s) 196, 214, 530, 550
HpyCH4III ACNGT 2 cut(s) 42, 202
HpyCH4IV ACGT 5 cut(s) 252, 352, 371, 387, 524
HpyCH4V TGCA 2 cut(s) 316, 587
HpyF10VI GCNNNNNNNGC 4 cut(s) 66, 174, 255, 374
HpySE526I ACGT 5 cut(s) 252, 352, 371, 387, 524
Hsp92I GRCGYC 3 cut(s) 296, 344, 524
Hsp92II CATG 3 cut(s) 80, 367, 571
HspAI GCGC 3 cut(s) 500, 514, 519
KspI CCGCGG 1 cut(s) 328
Kzo9I GATC 1 cut(s) 309
LmnI GCTCC 5 cut(s) 88, 174, 382, 399, 594
Lsp1109I GCAGC 1 cut(s) 45
LweI GCATC 1 cut(s) 574
MaeII ACGT 5 cut(s) 252, 352, 371, 387, 524
MalI GATC 1 cut(s) 311
MboI GATC 1 cut(s) 309
MhlI GDGCHC 6 cut(s) 93, 179, 200, 318, 360, 510
MlsI TGGCCA 1 cut(s) 368
MluCI AATT 1 cut(s) 405
MluNI TGGCCA 1 cut(s) 368
MlyI GAGTC 4 cut(s) 74, 413, 422, 461
MnlI CCTC 6 cut(s) 91, 129, 133, 210, 315, 564
Mox20I TGGCCA 1 cut(s) 368
MscI TGGCCA 1 cut(s) 368
MslI CAYNNNNRTG 1 cut(s) 75
Msp20I TGGCCA 1 cut(s) 368
MspA1I CMGCKG 2 cut(s) 60, 327
MspI CCGG 8 cut(s) 13, 86, 191, 380, 412, 562, 598, 603
MspR9I CCNGG 5 cut(s) 191, 413, 497, 563, 604
MteI GCGCNGCGC 1 cut(s) 517
MvaI CCWGG 1 cut(s) 497
MvnI CGCG 5 cut(s) 327, 502, 514, 516, 521
MwoI GCNNNNNNNGC 4 cut(s) 66, 174, 255, 374
NciI CCSGG 4 cut(s) 191, 413, 563, 604
NdeII GATC 1 cut(s) 309
NlaIII CATG 3 cut(s) 80, 367, 571
NlaIV GGNNCC 4 cut(s) 52, 176, 359, 596
NmeAIII GCCGAG 1 cut(s) 72
NspV TTCGAA 1 cut(s) 534
PcsI WCGNNNNNNNCGW 3 cut(s) 99, 249, 518
PfeI GAWTC 2 cut(s) 281, 478
PflFI GACNNNGTC 2 cut(s) 104, 295
PfoI TCCNGGA 1 cut(s) 189
PkrI GCNGC 5 cut(s) 32, 35, 62, 227, 518
PleI GAGTC 4 cut(s) 74, 413, 422, 461
PmaCI CACGTG 1 cut(s) 353
PmlI CACGTG 1 cut(s) 353
PpsI GAGTC 4 cut(s) 74, 413, 422, 461
Ppu21I YACGTR 1 cut(s) 353
PpuMI RGGWCCY 1 cut(s) 51
Psp124BI GAGCTC 1 cut(s) 93
Psp5II RGGWCCY 1 cut(s) 51
Psp6I CCWGG 1 cut(s) 495
PspCI CACGTG 1 cut(s) 353
PspGI CCWGG 1 cut(s) 495
PspN4I GGNNCC 4 cut(s) 52, 176, 359, 596
PspPI GGNCC 6 cut(s) 10, 51, 142, 223, 559, 600
PspPPI RGGWCCY 1 cut(s) 51
PsyI GACNNNGTC 2 cut(s) 104, 295
RsaI GTAC 1 cut(s) 494
RsaNI GTAC 1 cut(s) 493
RseI CAYNNNNRTG 1 cut(s) 75
Rsr2I CGGWCCG 1 cut(s) 600
RsrII CGGWCCG 1 cut(s) 600
SacI GAGCTC 1 cut(s) 93
SacII CCGCGG 1 cut(s) 328
SalI GTCGAC 2 cut(s) 128, 416
SatI GCNGC 5 cut(s) 31, 34, 61, 226, 517
Sau3AI GATC 1 cut(s) 309
Sau96I GGNCC 6 cut(s) 10, 51, 142, 223, 559, 600
SchI GAGTC 4 cut(s) 74, 413, 422, 461
ScrFI CCNGG 5 cut(s) 191, 413, 497, 563, 604
SduI GDGCHC 6 cut(s) 93, 179, 200, 318, 360, 510
SfaNI GCATC 1 cut(s) 574
SfcI CTRYAG 2 cut(s) 55, 64
Sfr303I CCGCGG 1 cut(s) 328
SfuI TTCGAA 1 cut(s) 534
SgrBI CCGCGG 1 cut(s) 328
SgrDI CGTCGACG 1 cut(s) 128
SinI GGWCC 3 cut(s) 51, 142, 600
SmiMI CAYNNNNRTG 1 cut(s) 75
Sse9I AATT 1 cut(s) 405
SsiI CCGC 9 cut(s) 31, 60, 226, 247, 258, 325, 327, 516, 574
SstI GAGCTC 1 cut(s) 93
StyD4I CCNGG 5 cut(s) 189, 411, 495, 561, 602
TaaI ACNGT 2 cut(s) 42, 202
TaiI ACGT 5 cut(s) 255, 355, 374, 390, 527
TaqI TCGA 4 cut(s) 113, 129, 417, 534
TasI AATT 1 cut(s) 405
TauI GCSGC 4 cut(s) 33, 63, 228, 519
TfiI GAWTC 2 cut(s) 281, 478
TscAI CASTG 1 cut(s) 594
TseI GCWGC 1 cut(s) 33
TspDTI ATGAA 1 cut(s) 453
TspRI CASTG 1 cut(s) 594
Tth111I GACNNNGTC 2 cut(s) 104, 295
VneI GTGCAC 1 cut(s) 314
VpaK11BI GGWCC 3 cut(s) 51, 142, 600
XapI RAATTY 1 cut(s) 405
XmiI GTMKAC 2 cut(s) 129, 417
ZraI GACGTC 1 cut(s) 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.