Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0015053

Physical Location & Seq

Genomic Coordinates Forward (+)

40593593 .. 40595830

Gene Structure 2238 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1325500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2238 bp

ATGAACAAGCTGAACAGCTCTTTCTTAAACCCCCTAATACCACCACAGCAAGCCACCGCGACTGCCACCACCAAAACTTTCAACGCCGTCATCAACCGCCTCTCGTCCCAAGGCTCTCACCAAGAAGTCCTTGCCACGTACTCCTCCATGCTCAAAACCAACACACCTCCAGACTCCTACACCTTGCCTAATCTTCTCAAAGCTTGCACCTCCTTAAACCTCTTTCTGTGTGGCCTCTCGCTCCACCAATGCATTGTCGTTAATGGGTTATCTTTGGATGCCTACATTGCATCTTCTCTGATCAACTTTTACGCCAAATTTGGACACGTCCAAAATGCCCGCAAGGTTTTCGACGTAATGCCCCACAGAAATGTTGTTCCTTGGACTTCCATTATCGGATGTTATTCGCTTGCCGGAAATGTTGGAGTTGCGTTTGAGATGTTTAGGGAGATGCGGCGCGAAGGAGTTCAGCCCAGTTCGGTTACTCTGTTAAGCTTGACCTCCGGAGTTTCGGAGCTGCCTTATTTGCAGTGTTTGCATGGTTGTGCAGTATTATATGGTTTTGAGTCTGACATTACTTTGGTGAATTCAATGTTGAATGTGTATGGTAAATGTGGAAGAGTTGAAGATGCTAGGCACTTGTTTGAGTATATGAATGCAAGAGACATAGTTTCATGGAATTCCTTGATTTCAAGCTATTCCCAGGCTGGAAATATCGGAGAAGTATTCCGGCTTTTGTATACGATGAGGGTTGAAGGAATGGAGCCTGATAAACAGACGTATGCATCTGCTGTATCTGTGGCTGCCATGCAGAGTAGTTTGAAATTGGGAAAGTCAGTGCACGGACAGATTTTAAGAACTGGATATGAATTGGATTCCCATGTCGAAACAGCGCTCATAGTTATGTACTTGAAATGCAGGAACATCGACACTGCATTTCATATCTTCGAAGGGACAAGGAATAAGGATGTGGTTCTGTGGACGGCCATGATGTCAGGGCTTGTGCAAAATGATTATGCGGACAGCGCACTAATCGTTCTCTCTCAAATGTTAGAATCAAAAACAGTGCCGTCTAGTGCGACCGTAGCTAGTGCTCTTGCAGCTTGTGCGCAACTGGGTTCTTTTGATATGGGAACCTCGGTTCATGGTTATGTGCTGAGGCAAGGAATGAGGCTAGATATCCCTGCCCAGAACTCCCTTGTAACAATGTATGCAAAGTGCGCGCGTTTGGAGCAGAGTCGTGCTGTTTTCGAAAGAATGGGCAAAAGAGATTTGGTTTCTTGGAATGCCATGATTGCTGGATATGCTCAAAACGGCCATTTACGCGAGGCTCTGCTTCTCTTCTCCGAGATGAGGGCAACCCTTCAGAAGCCCGATTCTTTAACTGTGGTCTCACTTCTGCAAGCTTGTGCTTCCATTGGGGCACTCCATCAAGGAAAGTGGATTCACAACTTTACAATTAGAAGTTGCCTTAGACCATGCATTTTGATAGACACAGCTCTGGTTGACATGTACTCAAAATGTGGTGACTTAGATAGAGCTCACAGGTGCTTCGTTGAGATGTCAGACCCAGATTTGGTCTCATGGAGCACGATTATTTCCGGTTATGGTTGCCATGGAAAAGCAGAGACCGCGTTGAGGATGTATTCAGAGTTTCTGCTCACTGGAATTAAACCAAACCATGTCGTCTTCCTGTCAATTCTTTCTGCTTGTAGTCACAATGGGCTTGTTGACACAGGCTTGAGCATATACCAGTCAATGATAGCAGACTTTGGCATTGCCCCGAGCCTTGAACACCGCGCTTGCATTGTTGATCTTCTCAGTCGAGCTGGCCGGGTGGAAGAGGCATATGACTTCTACAAGAGGGTTTTTCAAGAGCCGGCAGTCGATGTCTTGGGCATACTTCTTGACGCTTGTCGAACGAAGGGTAATGAGGAACTGGGGAACATTATTGCCGGAGAAATTCTGATGTTGCGACCTGGGGATGCTGGAAACTACGTGCAATTGGCACATAGCTACGCTTCGATGAACAGATGGGATGGCGTTGGCGAGGCATGGAGCCAAATGAGGTATCTTGGCTTGAAGAAACTCCCAGGCTGGAGTTGTATTGAGTTGCACGGGACGATCACTACATTCTACACAGATCACAATACAAATCCTCAGTTTGATGATATCGCGTCGATGTTGAAAATGTTAAGCGAGGGAATGAAGAAACCGAGCACTAACAACGAGAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF1068 PPR PPR_2

Protein Analysis

746

Amino Acids

82.15

Weight (kDa)

6.6

Isoelectric Point (pI)

40.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	24 - 69	1.5e-06	PPR repeat family
PPR_2	PF13041	123 - 168	3.5e-08	PPR repeat family
PPR	PF01535	127 - 156	8.2e-06	PPR repeat
PPR_2	PF13041	221 - 262	5.6e-09	PPR repeat family
PPR	PF01535	224 - 253	7.5e-06	PPR repeat
PPR_1	PF12854	422 - 452	6.1e-06	PPR repeat
PPR_2	PF13041	424 - 471	3.3e-11	PPR repeat family
PPR	PF01535	426 - 452	3.6e-08	PPR repeat
PPR_2	PF13041	524 - 572	8.5e-07	PPR repeat family
E_motif	PF20431	644 - 704	7.9e-07	E motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015053)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G04370
fragaria_vesca	FvH4_3g01610
malus_domestica	MD10G1325500.v1.1
prunus_persica	Prupe.4G016600_v2.0.a1
pyrus_communis	pycom10g27520
rosa_chinensis	RchiOBHm_Chr5g0002401
rosa_laevigata	RLG00000031029
rosa_multiflora	Rmu_co8285719.1_g000001 Rmu_sc0001717.1_g000003
rosa_roxburghii	Rroxscaffold_1G00073450
rosa_rugosa	Rorug04G0396000
rosa_samantha	Rh5AG018800 Rh5BG021600 Rh5CG020700 Rh5DG019800
rosa_wichuraiana	Rw5G001760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1110
AccI	GTMKAC	1 cut(s)	740
AccII	CGCG	8 cut(s)	59, 459, 1223, 1225, 1326, 1634, 1800, 2177
AccIII	TCCGGA	1 cut(s)	503
AciI	CCGC	7 cut(s)	57, 97, 340, 454, 1019, 1632, 1798
AcoI	YGGCCR	3 cut(s)	984, 1315, 1831
AcsI	RAATTY	4 cut(s)	317, 586, 679, 1962
AcuI	CTGAAG	1 cut(s)	1349
AfaI	GTAC	3 cut(s)	140, 908, 1514
AfeI	AGCGCT	1 cut(s)	894
AfiI	CCNNNNNNNGG	3 cut(s)	1353, 1576, 1638
AflIII	ACRYGT	2 cut(s)	325, 1509
AjiI	CACGTC	1 cut(s)	328
AjnI	CCWGG	3 cut(s)	702, 1978, 2092
AloI	GAACNNNNNNTCC	2 cut(s)	1125, 1157
Alw21I	GWGCWC	5 cut(s)	843, 1096, 1543, 1592, 2222
Alw26I	GTCTC	4 cut(s)	657, 1396, 1585, 1622
Alw44I	GTGCAC	1 cut(s)	839
Ama87I	CYCGRG	1 cut(s)	1783
Aor13HI	TCCGGA	1 cut(s)	503
Aor51HI	AGCGCT	1 cut(s)	894
AoxI	GGCC	4 cut(s)	232, 984, 1315, 1831
ApaLI	GTGCAC	1 cut(s)	839
ApeKI	GCWGC	3 cut(s)	517, 803, 1100
ApoI	RAATTY	4 cut(s)	317, 586, 679, 1962
Asp700I	GAANNNNTTC	1 cut(s)	465
AspLEI	GCGC	7 cut(s)	459, 895, 1028, 1111, 1223, 1225, 1802
AsuC2I	CCSGG	1 cut(s)	1835
AsuHPI	GGTGA	3 cut(s)	110, 595, 1538
AsuII	TTCGAA	2 cut(s)	948, 1251
AvaI	CYCGRG	1 cut(s)	1783
BaeGI	GKGCMC	2 cut(s)	843, 1426
BanII	GRGCYC	1 cut(s)	1543
BbsI	GAAGAC	1 cut(s)	1681
Bbv12I	GWGCWC	5 cut(s)	843, 1096, 1543, 1592, 2222
BbvCI	CCTCAGC	1 cut(s)	1157
BbvI	GCAGC	3 cut(s)	504, 790, 1112
BccI	CCATC	3 cut(s)	1437, 2028, 2033
BceAI	ACGGC	4 cut(s)	71, 999, 1054, 1330
BcgI	CGANNNNNNTGC	4 cut(s)	331, 365, 907, 941
BciT130I	CCWGG	3 cut(s)	704, 1980, 2094
BclI	TGATCA	1 cut(s)	300
BcnI	CCSGG	1 cut(s)	1835
BcoDI	GTCTC	4 cut(s)	657, 1396, 1585, 1622
BfaI	CTAG	4 cut(s)	633, 1074, 1089, 1175
BfoI	RGCGCY	1 cut(s)	896
BisI	GCNGC	4 cut(s)	455, 518, 804, 1101
BlsI	GCNGC	4 cut(s)	456, 519, 805, 1102
Bme1390I	CCNGG	4 cut(s)	704, 1835, 1980, 2094
BmeT110I	CYCGRG	1 cut(s)	1783
BmgBI	CACGTC	1 cut(s)	328
BmiI	GGNNCC	3 cut(s)	765, 1135, 2060
BmrFI	CCNGG	4 cut(s)	704, 1835, 1980, 2094
BmrI	ACTGGG	3 cut(s)	468, 1124, 1949
BmsI	GCATC	6 cut(s)	268, 299, 441, 619, 794, 1975
BmuI	ACTGGG	3 cut(s)	468, 1124, 1949
BoxI	GACNNNNGTC	1 cut(s)	1914
BpiI	GAAGAC	1 cut(s)	1681
BpmI	CTGGAG	2 cut(s)	153, 2119
Bpu10I	CCTNAGC	1 cut(s)	1157
Bpu14I	TTCGAA	2 cut(s)	948, 1251
BpuEI	CTTGAG	1 cut(s)	1762
BpuMI	CCSGG	1 cut(s)	1835
BsaAI	YACGTR	2 cut(s)	138, 1999
BsaI	GGTCTC	3 cut(s)	1396, 1585, 1622
BsaJI	CCNNGG	7 cut(s)	109, 380, 702, 1137, 1615, 1979, 2092
BsaWI	WCCGGW	2 cut(s)	503, 1601
Bsc4I	CCNNNNNNNGG	3 cut(s)	1353, 1576, 1638
Bse118I	RCCGGY	1 cut(s)	1879
Bse1I	ACTGG	6 cut(s)	474, 865, 1119, 1669, 1753, 1944
Bse3DI	GCAATG	2 cut(s)	285, 1776
BseAI	TCCGGA	1 cut(s)	503
BseBI	CCWGG	3 cut(s)	704, 1980, 2094
BseDI	CCNNGG	7 cut(s)	109, 380, 702, 1137, 1615, 1979, 2092
BseGI	GGATG	6 cut(s)	283, 404, 973, 1647, 1990, 2044
BseLI	CCNNNNNNNGG	3 cut(s)	1353, 1576, 1638
BseMI	GCAATG	2 cut(s)	285, 1776
BseMII	CTCAG	3 cut(s)	1148, 1834, 2174
BseNI	ACTGG	6 cut(s)	474, 865, 1119, 1669, 1753, 1944
BsePI	GCGCGC	1 cut(s)	1221
BseRI	GAGGAG	1 cut(s)	133
BseSI	GKGCMC	2 cut(s)	843, 1426
BseXI	GCAGC	3 cut(s)	504, 790, 1112
BsgI	GTGCAG	1 cut(s)	567
Bsh1236I	CGCG	8 cut(s)	59, 459, 1223, 1225, 1326, 1634, 1800, 2177
Bsh1285I	CGRYCG	1 cut(s)	1083
BshFI	GGCC	4 cut(s)	234, 986, 1317, 1833
BsiEI	CGRYCG	1 cut(s)	1083
BsiHKAI	GWGCWC	5 cut(s)	843, 1096, 1543, 1592, 2222
BsiHKCI	CYCGRG	1 cut(s)	1783
BsiSI	CCGG	7 cut(s)	414, 504, 730, 1602, 1834, 1880, 1956
BslFI	GGGAC	3 cut(s)	91, 967, 2134
BslI	CCNNNNNNNGG	3 cut(s)	1353, 1576, 1638
BsmAI	GTCTC	4 cut(s)	657, 1396, 1585, 1622
BsmFI	GGGAC	3 cut(s)	91, 967, 2134
BsmI	GAATGC	2 cut(s)	661, 1291
BsnI	GGCC	4 cut(s)	234, 986, 1317, 1833
Bso31I	GGTCTC	3 cut(s)	1396, 1585, 1622
BsoBI	CYCGRG	1 cut(s)	1783
Bsp119I	TTCGAA	2 cut(s)	948, 1251
Bsp1286I	GDGCHC	6 cut(s)	843, 1096, 1426, 1543, 1592, 2222
Bsp13I	TCCGGA	1 cut(s)	503
Bsp143I	GATC	4 cut(s)	300, 1813, 2124, 2143
Bsp19I	CCATGG	1 cut(s)	1615
BspACI	CCGC	7 cut(s)	57, 97, 340, 454, 1019, 1632, 1798
BspANI	GGCC	4 cut(s)	234, 986, 1317, 1833
BspCNI	CTCAG	3 cut(s)	1149, 1833, 2173
BspEI	TCCGGA	1 cut(s)	503
BspFNI	CGCG	8 cut(s)	59, 459, 1223, 1225, 1326, 1634, 1800, 2177
BspLI	GGNNCC	3 cut(s)	765, 1135, 2060
BspT104I	TTCGAA	2 cut(s)	948, 1251
BspTNI	GGTCTC	3 cut(s)	1396, 1585, 1622
BsrDI	GCAATG	2 cut(s)	285, 1776
BsrFI	RCCGGY	1 cut(s)	1879
BsrI	ACTGG	6 cut(s)	474, 865, 1119, 1669, 1753, 1944
BssAI	RCCGGY	1 cut(s)	1879
BssECI	CCNNGG	7 cut(s)	109, 380, 702, 1137, 1615, 1979, 2092
BssHII	GCGCGC	1 cut(s)	1221
BssMI	GATC	4 cut(s)	300, 1813, 2124, 2143
BssNAI	GTATAC	1 cut(s)	741
BssT1I	CCWWGG	3 cut(s)	109, 380, 1615
Bst1107I	GTATAC	1 cut(s)	741
Bst2UI	CCWGG	3 cut(s)	704, 1980, 2094
Bst4CI	ACNGT	3 cut(s)	1066, 1084, 1387
Bst6I	CTCTTC	3 cut(s)	613, 1346, 1836
BstAPI	GCANNNNNTGC	2 cut(s)	535, 1106
BstBAI	YACGTR	2 cut(s)	138, 1999
BstBI	TTCGAA	2 cut(s)	948, 1251
BstC8I	GCNNGC	9 cut(s)	51, 205, 340, 411, 1223, 1404, 1804, 1831, 1881
BstDEI	CTNAG	5 cut(s)	1157, 1472, 1531, 1820, 2160
BstDSI	CCRYGG	1 cut(s)	1615
BstF5I	GGATG	6 cut(s)	283, 404, 973, 1647, 1990, 2044
BstFNI	CGCG	8 cut(s)	59, 459, 1223, 1225, 1326, 1634, 1800, 2177
BstH2I	RGCGCY	1 cut(s)	896
BstHHI	GCGC	7 cut(s)	459, 895, 1028, 1111, 1223, 1225, 1802
BstKTI	GATC	4 cut(s)	303, 1816, 2127, 2146
BstMAI	GTCTC	4 cut(s)	657, 1396, 1585, 1622
BstMBI	GATC	4 cut(s)	300, 1813, 2124, 2143
BstMCI	CGRYCG	1 cut(s)	1083
BstNI	CCWGG	3 cut(s)	704, 1980, 2094
BstNSI	RCATGY	1 cut(s)	1513
BstPAI	GACNNNNGTC	1 cut(s)	1914
BstSCI	CCNGG	4 cut(s)	702, 1833, 1978, 2092
BstSLI	GKGCMC	2 cut(s)	843, 1426
BstUI	CGCG	8 cut(s)	59, 459, 1223, 1225, 1326, 1634, 1800, 2177
BstV1I	GCAGC	3 cut(s)	504, 790, 1112
BstV2I	GAAGAC	1 cut(s)	1681
BstZ17I	GTATAC	1 cut(s)	741
BsuRI	GGCC	4 cut(s)	234, 986, 1317, 1833
BtgI	CCRYGG	1 cut(s)	1615
BtrI	CACGTC	1 cut(s)	328
BtsCI	GGATG	6 cut(s)	283, 404, 973, 1647, 1990, 2044
BtsI	GCAGTG	2 cut(s)	536, 930
BtsIMutI	CAGTG	5 cut(s)	536, 843, 930, 1071, 1662
Cac8I	GCNNGC	9 cut(s)	51, 205, 340, 411, 1223, 1404, 1804, 1831, 1881
CfoI	GCGC	7 cut(s)	459, 895, 1028, 1111, 1223, 1225, 1802
Cfr10I	RCCGGY	1 cut(s)	1879
CseI	GACGC	2 cut(s)	1919, 2166
Csp6I	GTAC	3 cut(s)	139, 907, 1513
CspCI	CAANNNNNGTGG	2 cut(s)	1421, 1456
CviQI	GTAC	3 cut(s)	139, 907, 1513
DdeI	CTNAG	5 cut(s)	1157, 1472, 1531, 1820, 2160
DpnI	GATC	4 cut(s)	302, 1815, 2126, 2145
DpnII	GATC	4 cut(s)	300, 1813, 2124, 2143
EaeI	YGGCCR	3 cut(s)	984, 1315, 1831
Eam1104I	CTCTTC	3 cut(s)	613, 1346, 1836
EarI	CTCTTC	3 cut(s)	613, 1346, 1836
Ecl136II	GAGCTC	1 cut(s)	1541
Eco130I	CCWWGG	3 cut(s)	109, 380, 1615
Eco24I	GRGCYC	1 cut(s)	1543
Eco31I	GGTCTC	3 cut(s)	1396, 1585, 1622
Eco32I	GATATC	2 cut(s)	1180, 2173
Eco47III	AGCGCT	1 cut(s)	894
Eco53kI	GAGCTC	1 cut(s)	1541
Eco57I	CTGAAG	1 cut(s)	1349
Eco88I	CYCGRG	1 cut(s)	1783
EcoICRI	GAGCTC	1 cut(s)	1541
EcoRI	GAATTC	2 cut(s)	586, 679
EcoRII	CCWGG	3 cut(s)	702, 1978, 2092
EcoRV	GATATC	2 cut(s)	1180, 2173
EcoT14I	CCWWGG	3 cut(s)	109, 380, 1615
EcoT22I	ATGCAT	3 cut(s)	254, 787, 1484
EcoT38I	GRGCYC	1 cut(s)	1543
ErhI	CCWWGG	3 cut(s)	109, 380, 1615
FalI	AAGNNNNNCTT	2 cut(s)	114, 146
FaqI	GGGAC	3 cut(s)	91, 967, 2134
FauI	CCCGC	1 cut(s)	347
FauNDI	CATATG	1 cut(s)	1849
FbaI	TGATCA	1 cut(s)	300
FblI	GTMKAC	1 cut(s)	740
Fnu4HI	GCNGC	4 cut(s)	455, 518, 804, 1101
FokI	GGATG	6 cut(s)	290, 411, 980, 1654, 1997, 2051
FriOI	GRGCYC	1 cut(s)	1543
Fsp4HI	GCNGC	4 cut(s)	455, 518, 804, 1101
FspBI	CTAG	4 cut(s)	633, 1074, 1089, 1175
FspI	TGCGCA	1 cut(s)	1110
GlaI	GCGC	7 cut(s)	458, 894, 1027, 1110, 1222, 1224, 1801
GluI	GCNGC	4 cut(s)	455, 518, 804, 1101
GsuI	CTGGAG	2 cut(s)	153, 2119
HaeII	RGCGCY	1 cut(s)	896
HaeIII	GGCC	4 cut(s)	234, 986, 1317, 1833
HapII	CCGG	7 cut(s)	414, 504, 730, 1602, 1834, 1880, 1956
HgaI	GACGC	2 cut(s)	1919, 2166
HhaI	GCGC	7 cut(s)	459, 895, 1028, 1111, 1223, 1225, 1802
Hin6I	GCGC	7 cut(s)	457, 893, 1026, 1109, 1221, 1223, 1800
HinP1I	GCGC	7 cut(s)	457, 893, 1026, 1109, 1221, 1223, 1800
HincII	GTYRAC	2 cut(s)	1507, 1732
HindII	GTYRAC	2 cut(s)	1507, 1732
HindIII	AAGCTT	3 cut(s)	201, 493, 1404
HinfI	GANTC	7 cut(s)	173, 566, 875, 1055, 1237, 1376, 1444
HpaII	CCGG	7 cut(s)	414, 504, 730, 1602, 1834, 1880, 1956
HphI	GGTGA	3 cut(s)	110, 595, 1538
Hpy166II	GTNNAC	5 cut(s)	741, 841, 981, 1507, 1732
Hpy188III	TCNNGA	4 cut(s)	170, 504, 1874, 1907
Hpy8I	GTNNAC	5 cut(s)	741, 841, 981, 1507, 1732
Hpy99I	CGWCG	2 cut(s)	356, 2182
HpyAV	CCTTC	5 cut(s)	455, 749, 944, 1373, 1918
HpyCH4III	ACNGT	3 cut(s)	1066, 1084, 1387
HpyCH4IV	ACGT	5 cut(s)	137, 327, 354, 779, 1998
HpyF3I	CTNAG	5 cut(s)	1157, 1472, 1531, 1820, 2160
HpySE526I	ACGT	5 cut(s)	137, 327, 354, 779, 1998
HspAI	GCGC	7 cut(s)	457, 893, 1026, 1109, 1221, 1223, 1800
Kpn2I	TCCGGA	1 cut(s)	503
KroI	GCCGGC	1 cut(s)	1879
KroNI	GCCGGC	1 cut(s)	1881
Ksp22I	TGATCA	1 cut(s)	300
Kzo9I	GATC	4 cut(s)	300, 1813, 2124, 2143
LmnI	GCTCC	6 cut(s)	246, 514, 763, 1231, 1587, 2058
Lsp1109I	GCAGC	3 cut(s)	504, 790, 1112
LweI	GCATC	6 cut(s)	268, 299, 441, 619, 794, 1975
MaeI	CTAG	4 cut(s)	633, 1074, 1089, 1175
MaeII	ACGT	5 cut(s)	137, 327, 354, 779, 1998
MaeIII	GTNAC	4 cut(s)	481, 1201, 1526, 1715
MalI	GATC	4 cut(s)	302, 1815, 2126, 2145
MboI	GATC	4 cut(s)	300, 1813, 2124, 2143
MfeI	CAATTG	1 cut(s)	2003
MhlI	GDGCHC	6 cut(s)	843, 1096, 1426, 1543, 1592, 2222
MlyI	GAGTC	3 cut(s)	167, 575, 1246
MmeI	TCCRAC	1 cut(s)	403
Mph1103I	ATGCAT	3 cut(s)	254, 787, 1484
MroI	TCCGGA	1 cut(s)	503
MroNI	GCCGGC	1 cut(s)	1879
MroXI	GAANNNNTTC	1 cut(s)	465
MseI	TTAA	8 cut(s)	26, 215, 261, 491, 854, 1382, 1671, 2195
MslI	CAYNNNNRTG	4 cut(s)	369, 543, 902, 1149
MspI	CCGG	7 cut(s)	414, 504, 730, 1602, 1834, 1880, 1956
MspR9I	CCNGG	4 cut(s)	704, 1835, 1980, 2094
MunI	CAATTG	1 cut(s)	2003
Mva1269I	GAATGC	2 cut(s)	661, 1291
MvaI	CCWGG	3 cut(s)	704, 1980, 2094
MvnI	CGCG	8 cut(s)	59, 459, 1223, 1225, 1326, 1634, 1800, 2177
NaeI	GCCGGC	1 cut(s)	1881
NciI	CCSGG	1 cut(s)	1835
NcoI	CCATGG	1 cut(s)	1615
NdeI	CATATG	1 cut(s)	1849
NdeII	GATC	4 cut(s)	300, 1813, 2124, 2143
NgoMIV	GCCGGC	1 cut(s)	1879
NlaIV	GGNNCC	3 cut(s)	765, 1135, 2060
NmuCI	GTSAC	2 cut(s)	1526, 1715
NsbI	TGCGCA	1 cut(s)	1110
NsiI	ATGCAT	3 cut(s)	254, 787, 1484
NspI	RCATGY	1 cut(s)	1513
NspV	TTCGAA	2 cut(s)	948, 1251
PauI	GCGCGC	1 cut(s)	1221
PciI	ACATGT	1 cut(s)	1509
PctI	GAATGC	2 cut(s)	661, 1291
PdiI	GCCGGC	1 cut(s)	1881
PdmI	GAANNNNTTC	1 cut(s)	465
PfeI	GAWTC	4 cut(s)	875, 1055, 1376, 1444
PkrI	GCNGC	4 cut(s)	456, 519, 805, 1102
PleI	GAGTC	3 cut(s)	167, 574, 1245
PpsI	GAGTC	3 cut(s)	167, 574, 1245
Ppu21I	YACGTR	2 cut(s)	138, 1999
PscI	ACATGT	1 cut(s)	1509
PshAI	GACNNNNGTC	1 cut(s)	1914
Psp124BI	GAGCTC	1 cut(s)	1543
Psp6I	CCWGG	3 cut(s)	702, 1978, 2092
PspGI	CCWGG	3 cut(s)	702, 1978, 2092
PspN4I	GGNNCC	3 cut(s)	765, 1135, 2060
PteI	GCGCGC	1 cut(s)	1221
RsaI	GTAC	3 cut(s)	140, 908, 1514
RsaNI	GTAC	3 cut(s)	139, 907, 1513
RseI	CAYNNNNRTG	4 cut(s)	369, 543, 902, 1149
SacI	GAGCTC	1 cut(s)	1543
SaqAI	TTAA	8 cut(s)	26, 215, 261, 491, 854, 1382, 1671, 2195
SatI	GCNGC	4 cut(s)	455, 518, 804, 1101
Sau3AI	GATC	4 cut(s)	300, 1813, 2124, 2143
SchI	GAGTC	3 cut(s)	167, 575, 1246
ScrFI	CCNGG	4 cut(s)	704, 1835, 1980, 2094
SduI	GDGCHC	6 cut(s)	843, 1096, 1426, 1543, 1592, 2222
SfaNI	GCATC	6 cut(s)	268, 299, 441, 619, 794, 1975
SfuI	TTCGAA	2 cut(s)	948, 1251
SmiMI	CAYNNNNRTG	4 cut(s)	369, 543, 902, 1149
SmlI	CTYRAG	1 cut(s)	1741
SmoI	CTYRAG	1 cut(s)	1741
SsiI	CCGC	7 cut(s)	57, 97, 340, 454, 1019, 1632, 1798
SspMI	CTAG	4 cut(s)	633, 1074, 1089, 1175
SstI	GAGCTC	1 cut(s)	1543
StyD4I	CCNGG	4 cut(s)	702, 1833, 1978, 2092
StyI	CCWWGG	3 cut(s)	109, 380, 1615
TaaI	ACNGT	3 cut(s)	1066, 1084, 1387
TaiI	ACGT	5 cut(s)	140, 330, 357, 782, 2001
TatI	WGTACW	2 cut(s)	906, 1512
TauI	GCSGC	1 cut(s)	457
TfiI	GAWTC	4 cut(s)	875, 1055, 1376, 1444
Tru1I	TTAA	8 cut(s)	26, 215, 261, 491, 854, 1382, 1671, 2195
Tru9I	TTAA	8 cut(s)	26, 215, 261, 491, 854, 1382, 1671, 2195
TscAI	CASTG	5 cut(s)	536, 843, 937, 1071, 1669
TseFI	GTSAC	2 cut(s)	1526, 1715
TseI	GCWGC	3 cut(s)	517, 803, 1100
Tsp45I	GTSAC	2 cut(s)	1526, 1715
TspDTI	ATGAA	8 cut(s)	17, 663, 668, 882, 929, 1133, 2042, 2222
TspGWI	ACGGA	1 cut(s)	858
TspRI	CASTG	5 cut(s)	536, 843, 937, 1071, 1669
VneI	GTGCAC	1 cut(s)	839
XapI	RAATTY	4 cut(s)	317, 586, 679, 1962
XceI	RCATGY	1 cut(s)	1513
XmiI	GTMKAC	1 cut(s)	740
XmnI	GAANNNNTTC	1 cut(s)	465
XspI	CTAG	4 cut(s)	633, 1074, 1089, 1175
Zsp2I	ATGCAT	3 cut(s)	254, 787, 1484

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.