MD11G1044700.v1.1

Belongs to the actin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0004753
Physical Location & Seq
Reverse (-)
3926456 .. 3929195
2740 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1044700.v1.1.491

Sequence Viewer

Length: 723 bp
ATGACGACGTTGATCAGAAAAGTGTGGGAGTTGGTGTCAAGCCGCACGGTTTCGTGTTCAGAGTCAACGTCGTCGTCTTCTTCTTCGAGCTCTCCGTCGGAAACGGCGGGTTTCGGGGCGTTCGATCGAGTGCCGACAGACCTACTGATGCAAATCGCGAGACTGGTGGGGCCCAAGGAGCCAACATTCTTGGGTGAGAGTCGCCAGTTGTCCTACATGGGTATTTATGGTCAACGAGTTCCTGGTTTTGTTATCATTGACGGGGGCTCTGGCTATTGCAAGTTTGGCTGGAGCAAATACGCATGTCCATCAGGCCGATCAGCAACCTTTTTGGTGAAACCATCTTCGCAGCCCGTGGTTTTGTCTATACCAATCTGCCATTATGATGATACTGAATCTGCTAAGGCATCGAGACGGCAGCTCAAAGAAGCCATATACAAAGTACTTTTTGACATGAATGTCCCTGCTGTCGGTGCCGTTAATCAGGCAACTTTAGCTTTGTATGCAGCAAGACGAACTTCAGGGATTGTTGTAAACATTGGTTTCCAGGTCACTTCTATTGTTCCAATTTTAAATGGTAAAGTTATGCGCAAGGTGGGTGTGGAGGTTATGGGATTGGGAGCGCTGAAGCTTACAGGGTTCCGTAGGGAGCTATTGCAGCAGAGCAATATCAATTTTGACTCACTGTACACTGTTCGTGTTGTGCCTTGGCCCGTTCGTTAG

Protein Analysis

241

Amino Acids

26.13

Weight (kDa)

9.72

Isoelectric Point (pI)

60.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Actin PF00022 137 - 232 6.9e-13 Actin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 458
Acc16I TGCGCA 1 cut(s) 590
AccB1I GGYRCC 1 cut(s) 473
AccII CGCG 1 cut(s) 158
AciI CCGC 2 cut(s) 43, 107
AcuI CTGAAG 2 cut(s) 504, 647
AfaI GTAC 2 cut(s) 444, 689
AfeI AGCGCT 1 cut(s) 624
AfiI CCNNNNNNNGG 1 cut(s) 470
AjnI CCWGG 2 cut(s) 241, 546
AluBI AGCT 5 cut(s) 90, 421, 497, 631, 652
AluI AGCT 5 cut(s) 90, 421, 497, 631, 652
Alw21I GWGCWC 1 cut(s) 92
Alw26I GTCTC 2 cut(s) 154, 406
Aor51HI AGCGCT 1 cut(s) 624
AoxI GGCC 3 cut(s) 170, 313, 710
ApaI GGGCCC 1 cut(s) 174
ApeKI GCWGC 4 cut(s) 349, 418, 506, 658
AspLEI GCGC 2 cut(s) 591, 625
AspS9I GGNCC 3 cut(s) 170, 171, 711
AsuHPI GGTGA 2 cut(s) 206, 346
BaeGI GKGCMC 1 cut(s) 174
BanI GGYRCC 1 cut(s) 473
BanII GRGCYC 3 cut(s) 92, 174, 269
BbsI GAAGAC 1 cut(s) 69
Bbv12I GWGCWC 1 cut(s) 92
BbvI GCAGC 4 cut(s) 361, 430, 518, 670
BccI CCATC 2 cut(s) 316, 349
BceAI ACGGC 3 cut(s) 120, 431, 461
BciT130I CCWGG 2 cut(s) 243, 548
BclI TGATCA 1 cut(s) 12
BcoDI GTCTC 2 cut(s) 154, 406
BfoI RGCGCY 1 cut(s) 626
BisI GCNGC 5 cut(s) 43, 350, 419, 507, 659
BlsI GCNGC 5 cut(s) 44, 351, 420, 508, 660
BmcAI AGTACT 1 cut(s) 444
Bme1390I CCNGG 2 cut(s) 243, 548
BmgT120I GGNCC 3 cut(s) 170, 171, 711
BmiI GGNNCC 5 cut(s) 171, 172, 180, 475, 641
BmrFI CCNGG 2 cut(s) 243, 548
BmsI GCATC 2 cut(s) 138, 416
BpiI GAAGAC 1 cut(s) 69
BpmI CTGGAG 1 cut(s) 310
Bpu10I CCTNAGC 1 cut(s) 402
BsaBI GATNNNNATC 1 cut(s) 152
BsaJI CCNNGG 3 cut(s) 174, 354, 707
BsaXI ACNNNNNCTCC 2 cut(s) 20, 50
Bsc4I CCNNNNNNNGG 1 cut(s) 470
Bse1I ACTGG 2 cut(s) 168, 205
Bse8I GATNNNNATC 1 cut(s) 152
BseBI CCWGG 2 cut(s) 243, 548
BseDI CCNNGG 3 cut(s) 174, 354, 707
BseJI GATNNNNATC 1 cut(s) 152
BseLI CCNNNNNNNGG 1 cut(s) 470
BseNI ACTGG 2 cut(s) 168, 205
BseSI GKGCMC 1 cut(s) 174
BseXI GCAGC 4 cut(s) 361, 430, 518, 670
Bsh1236I CGCG 1 cut(s) 158
Bsh1285I CGRYCG 1 cut(s) 127
BshFI GGCC 3 cut(s) 172, 315, 712
BshNI GGYRCC 1 cut(s) 473
BsiEI CGRYCG 1 cut(s) 127
BsiHKAI GWGCWC 1 cut(s) 92
BslFI GGGAC 1 cut(s) 446
BslI CCNNNNNNNGG 1 cut(s) 470
BsmAI GTCTC 2 cut(s) 154, 406
BsmBI CGTCTC 1 cut(s) 406
BsmFI GGGAC 1 cut(s) 446
BsnI GGCC 3 cut(s) 172, 315, 712
Bsp120I GGGCCC 1 cut(s) 170
Bsp1286I GDGCHC 3 cut(s) 92, 174, 269
Bsp1407I TGTACA 1 cut(s) 687
Bsp143I GATC 3 cut(s) 12, 124, 317
Bsp68I TCGCGA 1 cut(s) 158
BspACI CCGC 2 cut(s) 43, 107
BspANI GGCC 3 cut(s) 172, 315, 712
BspFNI CGCG 1 cut(s) 158
BspLI GGNNCC 5 cut(s) 171, 172, 180, 475, 641
BspT107I GGYRCC 1 cut(s) 473
BsrGI TGTACA 1 cut(s) 687
BsrI ACTGG 2 cut(s) 168, 205
BssECI CCNNGG 3 cut(s) 174, 354, 707
BssMI GATC 3 cut(s) 12, 124, 317
BssT1I CCWWGG 2 cut(s) 174, 707
Bst2UI CCWGG 2 cut(s) 243, 548
Bst4CI ACNGT 3 cut(s) 49, 687, 694
BstAUI TGTACA 1 cut(s) 687
BstDEI CTNAG 1 cut(s) 402
BstDSI CCRYGG 1 cut(s) 354
BstFNI CGCG 1 cut(s) 158
BstH2I RGCGCY 1 cut(s) 626
BstHHI GCGC 2 cut(s) 591, 625
BstKTI GATC 3 cut(s) 15, 127, 320
BstMAI GTCTC 2 cut(s) 154, 406
BstMBI GATC 3 cut(s) 12, 124, 317
BstMCI CGRYCG 1 cut(s) 127
BstMWI GCNNNNNNNGC 6 cut(s) 178, 285, 473, 494, 503, 658
BstNI CCWGG 2 cut(s) 243, 548
BstNSI RCATGY 1 cut(s) 306
BstSCI CCNGG 2 cut(s) 241, 546
BstSLI GKGCMC 1 cut(s) 174
BstUI CGCG 1 cut(s) 158
BstV1I GCAGC 4 cut(s) 361, 430, 518, 670
BstV2I GAAGAC 1 cut(s) 69
BsuRI GGCC 3 cut(s) 172, 315, 712
BtgI CCRYGG 1 cut(s) 354
BtsIMutI CAGTG 2 cut(s) 683, 690
BtuMI TCGCGA 1 cut(s) 158
CfoI GCGC 2 cut(s) 591, 625
Cfr13I GGNCC 3 cut(s) 170, 171, 711
Csp6I GTAC 2 cut(s) 443, 688
CviAII CATG 3 cut(s) 217, 303, 454
CviQI GTAC 2 cut(s) 443, 688
DdeI CTNAG 1 cut(s) 402
DpnI GATC 3 cut(s) 14, 126, 319
DpnII GATC 3 cut(s) 12, 124, 317
DraI TTTAAA 1 cut(s) 573
DrdI GACNNNNNNGTC 1 cut(s) 458
DseDI GACNNNNNNGTC 1 cut(s) 458
Ecl136II GAGCTC 1 cut(s) 90
Eco130I CCWWGG 2 cut(s) 174, 707
Eco24I GRGCYC 3 cut(s) 92, 174, 269
Eco47III AGCGCT 1 cut(s) 624
Eco53kI GAGCTC 1 cut(s) 90
Eco57I CTGAAG 2 cut(s) 504, 647
EcoICRI GAGCTC 1 cut(s) 90
EcoO109I RGGNCCY 1 cut(s) 170
EcoRII CCWGG 2 cut(s) 241, 546
EcoT14I CCWWGG 2 cut(s) 174, 707
EcoT38I GRGCYC 3 cut(s) 92, 174, 269
ErhI CCWWGG 2 cut(s) 174, 707
Esp3I CGTCTC 1 cut(s) 406
FaeI CATG 3 cut(s) 220, 306, 457
FalI AAGNNNNNCTT 2 cut(s) 502, 534
FaqI GGGAC 1 cut(s) 446
FatI CATG 3 cut(s) 216, 302, 453
FauI CCCGC 1 cut(s) 100
FbaI TGATCA 1 cut(s) 12
Fnu4HI GCNGC 5 cut(s) 43, 350, 419, 507, 659
FriOI GRGCYC 3 cut(s) 92, 174, 269
Fsp4HI GCNGC 5 cut(s) 43, 350, 419, 507, 659
FspI TGCGCA 1 cut(s) 590
GlaI GCGC 2 cut(s) 590, 624
GluI GCNGC 5 cut(s) 43, 350, 419, 507, 659
GsuI CTGGAG 1 cut(s) 310
HaeII RGCGCY 1 cut(s) 626
HaeIII GGCC 3 cut(s) 172, 315, 712
HhaI GCGC 2 cut(s) 591, 625
Hin1II CATG 3 cut(s) 220, 306, 457
Hin6I GCGC 2 cut(s) 589, 623
HinP1I GCGC 2 cut(s) 589, 623
HincII GTYRAC 2 cut(s) 66, 233
HindII GTYRAC 2 cut(s) 66, 233
HindIII AAGCTT 1 cut(s) 629
HinfI GANTC 4 cut(s) 62, 199, 395, 680
HphI GGTGA 2 cut(s) 206, 346
Hpy166II GTNNAC 4 cut(s) 66, 233, 535, 690
Hpy188I TCNGA 3 cut(s) 17, 61, 100
Hpy188III TCNNGA 2 cut(s) 157, 411
Hpy8I GTNNAC 4 cut(s) 66, 233, 535, 690
Hpy99I CGWCG 4 cut(s) 10, 73, 76, 100
HpyCH4III ACNGT 3 cut(s) 49, 687, 694
HpyCH4IV ACGT 2 cut(s) 8, 68
HpyCH4V TGCA 4 cut(s) 151, 279, 506, 658
HpyF10VI GCNNNNNNNGC 6 cut(s) 178, 285, 473, 494, 503, 658
HpyF3I CTNAG 1 cut(s) 402
HpySE526I ACGT 2 cut(s) 8, 68
Hsp92II CATG 3 cut(s) 220, 306, 457
HspAI GCGC 2 cut(s) 589, 623
Ksp22I TGATCA 1 cut(s) 12
Kzo9I GATC 3 cut(s) 12, 124, 317
LmnI GCTCC 4 cut(s) 178, 291, 620, 649
Lsp1109I GCAGC 4 cut(s) 361, 430, 518, 670
LweI GCATC 2 cut(s) 138, 416
MaeII ACGT 2 cut(s) 8, 68
MaeIII GTNAC 1 cut(s) 550
MalI GATC 3 cut(s) 14, 126, 319
MboI GATC 3 cut(s) 12, 124, 317
MboII GAAGA 4 cut(s) 69, 72, 75, 336
MhlI GDGCHC 3 cut(s) 92, 174, 269
MluCI AATT 2 cut(s) 567, 673
MlyI GAGTC 3 cut(s) 71, 208, 674
MmeI TCCRAC 1 cut(s) 78
MnlI CCTC 1 cut(s) 598
MseI TTAA 2 cut(s) 480, 572
MslI CAYNNNNRTG 1 cut(s) 384
MspR9I CCNGG 2 cut(s) 243, 548
MvaI CCWGG 2 cut(s) 243, 548
MvnI CGCG 1 cut(s) 158
MwoI GCNNNNNNNGC 6 cut(s) 178, 285, 473, 494, 503, 658
NdeII GATC 3 cut(s) 12, 124, 317
NlaIII CATG 3 cut(s) 220, 306, 457
NlaIV GGNNCC 5 cut(s) 171, 172, 180, 475, 641
NmuCI GTSAC 1 cut(s) 550
NruI TCGCGA 1 cut(s) 158
NsbI TGCGCA 1 cut(s) 590
NspI RCATGY 1 cut(s) 306
PcsI WCGNNNNNNNCGW 2 cut(s) 92, 120
PfeI GAWTC 1 cut(s) 395
PkrI GCNGC 5 cut(s) 44, 351, 420, 508, 660
Ple19I CGATCG 1 cut(s) 127
PleI GAGTC 3 cut(s) 70, 207, 674
PpsI GAGTC 3 cut(s) 70, 207, 674
Psp124BI GAGCTC 1 cut(s) 92
Psp6I CCWGG 2 cut(s) 241, 546
PspGI CCWGG 2 cut(s) 241, 546
PspN4I GGNNCC 5 cut(s) 171, 172, 180, 475, 641
PspOMI GGGCCC 1 cut(s) 170
PspPI GGNCC 3 cut(s) 170, 171, 711
PvuI CGATCG 1 cut(s) 127
RruI TCGCGA 1 cut(s) 158
RsaI GTAC 2 cut(s) 444, 689
RsaNI GTAC 2 cut(s) 443, 688
RseI CAYNNNNRTG 1 cut(s) 384
SacI GAGCTC 1 cut(s) 92
SaqAI TTAA 2 cut(s) 480, 572
SatI GCNGC 5 cut(s) 43, 350, 419, 507, 659
Sau3AI GATC 3 cut(s) 12, 124, 317
Sau96I GGNCC 3 cut(s) 170, 171, 711
ScaI AGTACT 1 cut(s) 444
SchI GAGTC 3 cut(s) 71, 208, 674
ScrFI CCNGG 2 cut(s) 243, 548
SduI GDGCHC 3 cut(s) 92, 174, 269
SfaNI GCATC 2 cut(s) 138, 416
SmiMI CAYNNNNRTG 1 cut(s) 384
Sse9I AATT 2 cut(s) 567, 673
SsiI CCGC 2 cut(s) 43, 107
SstI GAGCTC 1 cut(s) 92
StyD4I CCNGG 2 cut(s) 241, 546
StyI CCWWGG 2 cut(s) 174, 707
TaaI ACNGT 3 cut(s) 49, 687, 694
TaiI ACGT 2 cut(s) 11, 71
TaqI TCGA 4 cut(s) 86, 123, 127, 410
TasI AATT 2 cut(s) 567, 673
TatI WGTACW 2 cut(s) 442, 687
TauI GCSGC 1 cut(s) 45
TfiI GAWTC 1 cut(s) 395
Tru1I TTAA 2 cut(s) 480, 572
Tru9I TTAA 2 cut(s) 480, 572
TscAI CASTG 2 cut(s) 690, 697
TseFI GTSAC 1 cut(s) 550
TseI GCWGC 4 cut(s) 349, 418, 506, 658
Tsp45I GTSAC 1 cut(s) 550
TspDTI ATGAA 1 cut(s) 470
TspGWI ACGGA 2 cut(s) 84, 632
TspRI CASTG 2 cut(s) 690, 697
XceI RCATGY 1 cut(s) 306
ZrmI AGTACT 1 cut(s) 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.