Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0008856

Physical Location & Seq

Genomic Coordinates Reverse (-)

9679551 .. 9683510

Gene Structure 3960 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1109700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2388 bp

ATGGGAAAGCAGTTGGAACGCCTGGACTCGAGTGTTGGGGGCAACCGTCCAGGCTGCATGTGGAGCCTATTGAACATCCTTGATTACCATCATTGGAGTAATGTCAAGAAGATTCTGCCCCACAGAAAGCGGGCCGGAGGAAAGCGAGTCAGACGCAATTATGGAAGTCGAAAAGCAACTTCGGAGAACCGTGAAATTGGTAAAAGGCCAGAGTTGGTAGTTGCGGAAGCTGAACCTCTGTTGGTCAAACAGCAGAGTACAGAAACTAGCACAGCAAAGAAAAGGTCTTGTAAATCTCGCAAGAAAGCATCAAGTGCAAAAGACAAACCAAGGGAAGAAAGCCCCAAAAGACGGATTTTAAGCTGCCCTGTACAGTCATGGTTGCGAAGGACTAGTGTCGTACATGATGCAGAGCCTTCGGAAAATTGTTTGGATACAACAAGAAACGGCAGTATCACTAGTCCTAGAGGATTGCAGGATTCATCGCACTTGAAGATGTCGAAGAATACAATGAAACGGGAACTTCCTCTCAAGGACGTTTCCAGTGACACAGTTAATGATCATGTTGACATTTTGGAGTTATTCAAAGTAAACAAGGAGTTTTTCTTAAAAATCCTACAAGATCCAGAAATTAGTACAAACCAGTTTCCAGGCCTGAAAAATTCCAAGAACAAAGTCAGATTAACAAAATCAAGGTCATTCCCCTTCACTGATTCCTCTCGAGCGAGAAATAGGAGGCCTAGCACACTCAAACACAAGCAGAATGAAGTTTGGTCCTTCCCAAAAGGAGAAAAATTGCTTGCCACTACTCAGACACCGAAGTCAATGACATCCGAAACTCAGGAAGACGATTCTATGAAGTCCAAGCATTACATGGCGGGTAATAACAGTGTAGGCAGTACCATGCAACAAGAAACAAGCTTTTCTTCTTTGGGATTGCCTGAGGAAGTTAGTCACCAAGGATGGAATCAATTGGTCATAAATCGTTTCAGACATATAACGCAGAAGCTAATGCATGCGCTACAAGAGAGCAAGAAGGAAAATTATGGCAATTCCATTGAGTCTCTTTTCTGGAAAGACCCTTCTGGACGTGATGGCAAAGAAGTGCTTGAAACGCTGGAGTTAACTACGACACAAGAGGGAAACAAGGTTCATGGTTTAGATGATAATCTTGGGCGGCAAAGACGTCATCGTGTAAGAAGAACCTCCTCTCTAGATGAGTCGCTTAGTAGATATACTCAGTTGTATGAGAGCAGCTACAGCAGTGATCAGAGAAACTTGCATCACTCGAAGAGCTTAAAGTTGAAAAGTGAAGAAAAAGTTCCTTCAACTGGGAATGCTCAGAAAACCACCAGAAGGAATCTGTCACTGCCTGATCTTGACTCTTTTTCTTCCATCTTAAACGGGGCATTGGTTAAAGATGCTTTTCGTTTAGGGATGCCGGTTACAAAAGCTGCGGAACAAAATACCGATAAGTCTGAACAACTCGATGCTATTTTAGAAAGTGACGACAGTGCACATATTGCTGACATAAAGGACAGTGATGAGCATGCAGTCGTGCCATTAGTAGGAGAAAGCACAGCTCACCAGGAGCAAGAGATGGTTATTGCGATGAGTCGTGAAAGCGAGCAAGCACAGCCGATGGAAAGTTCTAATCTTGATCCCCCAGACTATATAACCAGCAATGCAAGGTTGTCAATCTCAGAAGGTTCAGAGTTAAATCCCGGAAGTTCCCATGCGAATGAGCCGAATTCTTCTCTTGACCTACAAGGCAGGCACATCATAGATTCGTTACCGGAATCCTCTATAAGCACTGAAATTGAACATAAGAGTTCAATCACTCACTCCTCGCGCTTTGTACTGGACAAAGTGGATGATCCTGATTCCGAATTCAGTTACGTGAGATATGTTCTTGAGCTCTCAGGCTTCATTGGTCACGAGCAGCTCGGAGCATGGTACTCACTAGACCAGCCTCTGGATCCCACGATGTTCATGGAATTGGAGGATTGCTTTCAGCATAAACCCGAATCCTACGAAGATCAAGTTACATCTGGCGATAGTGATCACCGGCTTCTATTTGATTTAGTTAATGAGACCCTCCTAGAGGTCTATGAGAGGTCATACACCTACTTCCCAAGGGCCTTGTCCTTCAGTGGCAGCATTCGTCCAATGCCGAAGGGGCAACGTCTTCTTGACGATGTCTGGACAAGAGTTAGCTCGTACCTGAGCTTGAGGCCGGAGATGGATCAATCACTGGATGATGTTGTGGCTCGAGATTTGGCGAAGGGCGATCGTTGGATGAACCTTCAATGGGAAACTGAGTGTGTGGCACTTGAACTCGAGGATTTGATCTTTGATGGACTATTAGATGAAGTAATTTGTGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3741 DUF4378

Protein Analysis

796

Amino Acids

90.16

Weight (kDa)

6.13

Isoelectric Point (pI)

61.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF3741	PF12552	180 - 209	1.9e-06	Protein of unknown function (DUF3741)
DUF4378	PF14309	630 - 791	2e-41	Domain of unknown function (DUF4378)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008856)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g36010 FvH4_3g36010 FvH4_3g36010
malus_domestica	MD11G1109700.v1.1
prunus_persica	Prupe.6G078200_v2.0.a1 Prupe.6G078200_v2.0.a1
pyrus_communis	pycom03g07730 pycom11g09050
rosa_chinensis	RchiOBHm_Chr5g0064421
rosa_laevigata	RLG00000035723
rosa_multiflora	Rmu_sc0001125.1_g000006
rosa_roxburghii	Rroxscaffold_1G00016570
rosa_rugosa	Rorug05G0364600 Rorug05G0364700.1 Rorug05G0364800 Rorug05G0364800 Rorug05G0364800 Rorug05G0364900
rosa_samantha	Rh5AG423200 Rh5BG438700 Rh5CG461400 Rh5DG452000
rosa_wichuraiana	Rw5G039780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	820
AatII	GACGTC	1 cut(s)	1189
AccB7I	CCANNNNNTGG	1 cut(s)	1975
AccII	CGCG	1 cut(s)	1852
AciI	CCGC	5 cut(s)	130, 224, 878, 1177, 1457
AclWI	GGATC	6 cut(s)	617, 1655, 1871, 1973, 1986, 2253
AcsI	RAATTY	3 cut(s)	661, 1750, 1889
AcuI	CTGAAG	1 cut(s)	2134
AcyI	GRCGYC	1 cut(s)	1186
AfaI	GTAC	8 cut(s)	259, 372, 402, 637, 901, 1860, 1958, 2222
AfiI	CCNNNNNNNGG	7 cut(s)	351, 1331, 1356, 1568, 1975, 2104, 2311
AhlI	ACTAGT	2 cut(s)	392, 458
AjiI	CACGTC	1 cut(s)	1091
AjnI	CCWGG	4 cut(s)	21, 49, 649, 1587
AloI	GAACNNNNNNTCC	2 cut(s)	1134, 1166
Alw21I	GWGCWC	2 cut(s)	1519, 1920
Alw26I	GTCTC	2 cut(s)	1068, 2087
Alw44I	GTGCAC	1 cut(s)	1515
AlwI	GGATC	6 cut(s)	617, 1655, 1871, 1973, 1986, 2253
AlwNI	CAGNNNCTG	1 cut(s)	1975
Ama87I	CYCGRG	4 cut(s)	28, 720, 2271, 2339
AoxI	GGCC	6 cut(s)	132, 206, 652, 737, 2139, 2234
ApaLI	GTGCAC	1 cut(s)	1515
ApeKI	GCWGC	6 cut(s)	54, 363, 1254, 1454, 1942, 2157
ApoI	RAATTY	3 cut(s)	661, 1750, 1889
ArsI	GACNNNNNNTTYG	4 cut(s)	269, 301, 680, 712
Asp700I	GAANNNNTTC	1 cut(s)	1320
AspLEI	GCGC	2 cut(s)	1021, 1854
AspS9I	GGNCC	3 cut(s)	132, 774, 2139
AsuC2I	CCSGG	1 cut(s)	1725
AsuHPI	GGTGA	3 cut(s)	947, 1577, 2057
AvaI	CYCGRG	4 cut(s)	28, 720, 2271, 2339
AvaII	GGWCC	1 cut(s)	774
AxyI	CCTNAGG	1 cut(s)	942
BaeGI	GKGCMC	1 cut(s)	1519
BaeI	ACNNNNGTAYC	2 cut(s)	436, 469
BamHI	GGATCC	1 cut(s)	1978
BanII	GRGCYC	1 cut(s)	1920
BauI	CACGAG	1 cut(s)	1937
BbsI	GAAGAC	2 cut(s)	852, 2180
Bbv12I	GWGCWC	2 cut(s)	1519, 1920
BbvI	GCAGC	6 cut(s)	41, 350, 1266, 1441, 1954, 2169
BccI	CCATC	8 cut(s)	96, 957, 1088, 1403, 1594, 1636, 2236, 2351
BceAI	ACGGC	1 cut(s)	463
BcgI	CGANNNNNNTGC	2 cut(s)	399, 433
BciT130I	CCWGG	4 cut(s)	23, 51, 651, 1589
BciVI	GTATCC	1 cut(s)	427
BclI	TGATCA	3 cut(s)	559, 1267, 2062
BcnI	CCSGG	1 cut(s)	1725
BcoDI	GTCTC	2 cut(s)	1068, 2087
BcuI	ACTAGT	2 cut(s)	392, 458
BfaI	CTAG	8 cut(s)	267, 393, 459, 465, 741, 1214, 1964, 2102
BfmI	CTRYAG	1 cut(s)	1258
BfuI	GTATCC	1 cut(s)	427
BglI	GCCNNNNNGGC	1 cut(s)	2179
BisI	GCNGC	7 cut(s)	55, 364, 1178, 1255, 1455, 1943, 2158
BlsI	GCNGC	7 cut(s)	56, 365, 1179, 1256, 1456, 1944, 2159
Bme1390I	CCNGG	5 cut(s)	23, 51, 651, 1589, 1725
Bme18I	GGWCC	1 cut(s)	774
BmeT110I	CYCGRG	4 cut(s)	28, 720, 2271, 2339
BmgBI	CACGTC	1 cut(s)	1091
BmgT120I	GGNCC	3 cut(s)	132, 774, 2139
BmiI	GGNNCC	2 cut(s)	65, 1980
BmrFI	CCNGG	5 cut(s)	23, 51, 651, 1589, 1725
BmrI	ACTGGG	1 cut(s)	1341
BmsI	GCATC	6 cut(s)	317, 397, 1291, 1411, 1428, 1480
BmuI	ACTGGG	1 cut(s)	1341
BoxI	GACNNNNGTC	1 cut(s)	395
BpiI	GAAGAC	2 cut(s)	852, 2180
BpmI	CTGGAG	1 cut(s)	1139
Bpu10I	CCTNAGC	1 cut(s)	2225
BpuEI	CTTGAG	3 cut(s)	515, 1934, 2251
BpuMI	CCSGG	1 cut(s)	1725
BsaAI	YACGTR	1 cut(s)	1900
BsaBI	GATNNNNATC	3 cut(s)	87, 1167, 2061
BsaHI	GRCGYC	1 cut(s)	1186
BsaI	GGTCTC	1 cut(s)	2087
BsaJI	CCNNGG	3 cut(s)	329, 958, 2135
BsaWI	WCCGGW	1 cut(s)	1795
Bsc4I	CCNNNNNNNGG	7 cut(s)	351, 1331, 1356, 1568, 1975, 2104, 2311
Bse118I	RCCGGY	2 cut(s)	1441, 2067
Bse1I	ACTGG	5 cut(s)	543, 643, 1336, 1866, 2259
Bse21I	CCTNAGG	1 cut(s)	942
Bse3DI	GCAATG	1 cut(s)	1690
Bse8I	GATNNNNATC	3 cut(s)	87, 1167, 2061
BseBI	CCWGG	4 cut(s)	23, 51, 651, 1589
BseDI	CCNNGG	3 cut(s)	329, 958, 2135
BseGI	GGATG	7 cut(s)	75, 830, 968, 1443, 1879, 2263, 2304
BseJI	GATNNNNATC	3 cut(s)	87, 1167, 2061
BseLI	CCNNNNNNNGG	7 cut(s)	351, 1331, 1356, 1568, 1975, 2104, 2311
BseMI	GCAATG	1 cut(s)	1690
BseMII	CTCAG	9 cut(s)	824, 854, 933, 1253, 1355, 1716, 1935, 2216, 2310
BseNI	ACTGG	5 cut(s)	543, 643, 1336, 1866, 2259
BseRI	GAGGAG	2 cut(s)	1198, 1837
BseSI	GKGCMC	1 cut(s)	1519
BseXI	GCAGC	6 cut(s)	41, 350, 1266, 1441, 1954, 2169
Bsh1236I	CGCG	1 cut(s)	1852
Bsh1285I	CGRYCG	1 cut(s)	2293
BshFI	GGCC	6 cut(s)	134, 208, 654, 739, 2141, 2236
BsiEI	CGRYCG	1 cut(s)	2293
BsiHKAI	GWGCWC	2 cut(s)	1519, 1920
BsiHKCI	CYCGRG	4 cut(s)	28, 720, 2271, 2339
BsiSI	CCGG	6 cut(s)	135, 1442, 1725, 1796, 2068, 2237
BslI	CCNNNNNNNGG	7 cut(s)	351, 1331, 1356, 1568, 1975, 2104, 2311
BsmAI	GTCTC	2 cut(s)	1068, 2087
BsmI	GAATGC	2 cut(s)	1342, 2160
BsnI	GGCC	6 cut(s)	134, 208, 654, 739, 2141, 2236
Bso31I	GGTCTC	1 cut(s)	2087
BsoBI	CYCGRG	4 cut(s)	28, 720, 2271, 2339
Bsp1286I	GDGCHC	2 cut(s)	1519, 1920
Bsp1407I	TGTACA	1 cut(s)	370
BspACI	CCGC	5 cut(s)	130, 224, 878, 1177, 1457
BspANI	GGCC	6 cut(s)	134, 208, 654, 739, 2141, 2236
BspCNI	CTCAG	9 cut(s)	823, 853, 934, 1252, 1354, 1715, 1934, 2217, 2311
BspFNI	CGCG	1 cut(s)	1852
BspLI	GGNNCC	2 cut(s)	65, 1980
BspPI	GGATC	6 cut(s)	617, 1655, 1871, 1973, 1986, 2253
BspQI	GCTCTTC	1 cut(s)	1286
BspTNI	GGTCTC	1 cut(s)	2087
BsrDI	GCAATG	1 cut(s)	1690
BsrFI	RCCGGY	2 cut(s)	1441, 2067
BsrGI	TGTACA	1 cut(s)	370
BsrI	ACTGG	5 cut(s)	543, 643, 1336, 1866, 2259
BssAI	RCCGGY	2 cut(s)	1441, 2067
BssECI	CCNNGG	3 cut(s)	329, 958, 2135
BssNI	GRCGYC	1 cut(s)	1186
BssSI	CACGAG	1 cut(s)	1937
BssT1I	CCWWGG	3 cut(s)	329, 958, 2135
Bst2BI	CACGAG	1 cut(s)	1937
Bst2UI	CCWGG	4 cut(s)	23, 51, 651, 1589
Bst4CI	ACNGT	7 cut(s)	47, 191, 375, 553, 890, 1514, 1541
Bst6I	CTCTTC	1 cut(s)	1286
BstACI	GRCGYC	1 cut(s)	1186
BstAPI	GCANNNNNTGC	2 cut(s)	314, 1523
BstAUI	TGTACA	1 cut(s)	370
BstBAI	YACGTR	1 cut(s)	1900
BstC8I	GCNNGC	7 cut(s)	132, 801, 1017, 1551, 1628, 1632, 1775
BstENI	CCTNNNNNAGG	1 cut(s)	2102
BstF5I	GGATG	7 cut(s)	75, 830, 968, 1443, 1879, 2263, 2304
BstFNI	CGCG	1 cut(s)	1852
BstHHI	GCGC	2 cut(s)	1021, 1854
BstMAI	GTCTC	2 cut(s)	1068, 2087
BstMCI	CGRYCG	1 cut(s)	2293
BstMWI	GCNNNNNNNGC	7 cut(s)	63, 314, 1114, 1260, 1523, 1636, 2179
BstNI	CCWGG	4 cut(s)	23, 51, 651, 1589
BstNSI	RCATGY	3 cut(s)	61, 1019, 1553
BstPAI	GACNNNNGTC	1 cut(s)	395
BstSCI	CCNGG	5 cut(s)	21, 49, 649, 1587, 1723
BstSFI	CTRYAG	1 cut(s)	1258
BstSLI	GKGCMC	1 cut(s)	1519
BstUI	CGCG	1 cut(s)	1852
BstV1I	GCAGC	6 cut(s)	41, 350, 1266, 1441, 1954, 2169
BstV2I	GAAGAC	2 cut(s)	852, 2180
BstX2I	RGATCY	2 cut(s)	622, 1978
BstYI	RGATCY	2 cut(s)	622, 1978
Bsu36I	CCTNAGG	1 cut(s)	942
BsuI	GTATCC	1 cut(s)	427
BsuRI	GGCC	6 cut(s)	134, 208, 654, 739, 2141, 2236
BtgZI	GCGATG	2 cut(s)	468, 1625
BtrI	CACGTC	1 cut(s)	1091
BtsCI	GGATG	7 cut(s)	75, 830, 968, 1443, 1879, 2263, 2304
BtsI	GCAGTG	2 cut(s)	1270, 1367
Cac8I	GCNNGC	7 cut(s)	132, 801, 1017, 1551, 1628, 1632, 1775
CaiI	CAGNNNCTG	1 cut(s)	1975
CfoI	GCGC	2 cut(s)	1021, 1854
Cfr10I	RCCGGY	2 cut(s)	1441, 2067
Cfr13I	GGNCC	3 cut(s)	132, 774, 2139
CseI	GACGC	1 cut(s)	162
Csp6I	GTAC	8 cut(s)	258, 371, 401, 636, 900, 1859, 1957, 2221
CviQI	GTAC	8 cut(s)	258, 371, 401, 636, 900, 1859, 1957, 2221
DrdI	GACNNNNNNGTC	1 cut(s)	820
DseDI	GACNNNNNNGTC	1 cut(s)	820
Eam1104I	CTCTTC	1 cut(s)	1286
EarI	CTCTTC	1 cut(s)	1286
Ecl136II	GAGCTC	1 cut(s)	1918
Eco130I	CCWWGG	3 cut(s)	329, 958, 2135
Eco147I	AGGCCT	2 cut(s)	654, 739
Eco24I	GRGCYC	1 cut(s)	1920
Eco31I	GGTCTC	1 cut(s)	2087
Eco47I	GGWCC	1 cut(s)	774
Eco53kI	GAGCTC	1 cut(s)	1918
Eco57I	CTGAAG	1 cut(s)	2134
Eco81I	CCTNAGG	1 cut(s)	942
Eco88I	CYCGRG	4 cut(s)	28, 720, 2271, 2339
EcoICRI	GAGCTC	1 cut(s)	1918
EcoNI	CCTNNNNNAGG	1 cut(s)	2102
EcoO109I	RGGNCCY	1 cut(s)	2139
EcoRI	GAATTC	2 cut(s)	1750, 1889
EcoRII	CCWGG	4 cut(s)	21, 49, 649, 1587
EcoT14I	CCWWGG	3 cut(s)	329, 958, 2135
EcoT22I	ATGCAT	1 cut(s)	1017
EcoT38I	GRGCYC	1 cut(s)	1920
ErhI	CCWWGG	3 cut(s)	329, 958, 2135
FalI	AAGNNNNNCTT	4 cut(s)	910, 942, 1092, 1124
FauI	CCCGC	2 cut(s)	123, 871
FbaI	TGATCA	3 cut(s)	559, 1267, 2062
Fnu4HI	GCNGC	7 cut(s)	55, 364, 1178, 1255, 1455, 1943, 2158
FokI	GGATG	7 cut(s)	62, 817, 975, 1450, 1886, 2270, 2311
FriOI	GRGCYC	1 cut(s)	1920
Fsp4HI	GCNGC	7 cut(s)	55, 364, 1178, 1255, 1455, 1943, 2158
FspBI	CTAG	8 cut(s)	267, 393, 459, 465, 741, 1214, 1964, 2102
GlaI	GCGC	2 cut(s)	1020, 1853
GluI	GCNGC	7 cut(s)	55, 364, 1178, 1255, 1455, 1943, 2158
GsuI	CTGGAG	1 cut(s)	1139
HaeIII	GGCC	6 cut(s)	134, 208, 654, 739, 2141, 2236
HapII	CCGG	6 cut(s)	135, 1442, 1725, 1796, 2068, 2237
HgaI	GACGC	1 cut(s)	162
HhaI	GCGC	2 cut(s)	1021, 1854
Hin1I	GRCGYC	1 cut(s)	1186
Hin6I	GCGC	2 cut(s)	1019, 1852
HinP1I	GCGC	2 cut(s)	1019, 1852
HincII	GTYRAC	2 cut(s)	568, 1125
HindII	GTYRAC	2 cut(s)	568, 1125
HindIII	AAGCTT	1 cut(s)	919
HpaI	GTTAAC	1 cut(s)	1125
HpaII	CCGG	6 cut(s)	135, 1442, 1725, 1796, 2068, 2237
HphI	GGTGA	3 cut(s)	947, 1577, 2057
Hpy166II	GTNNAC	4 cut(s)	568, 592, 1125, 1517
Hpy8I	GTNNAC	4 cut(s)	568, 592, 1125, 1517
HpyCH4III	ACNGT	7 cut(s)	47, 191, 375, 553, 890, 1514, 1541
HpyCH4IV	ACGT	5 cut(s)	537, 1090, 1186, 1899, 2185
HpyF10VI	GCNNNNNNNGC	7 cut(s)	63, 314, 1114, 1260, 1523, 1636, 2179
HpySE526I	ACGT	5 cut(s)	537, 1090, 1186, 1899, 2185
Hsp92I	GRCGYC	1 cut(s)	1186
HspAI	GCGC	2 cut(s)	1019, 1852
Ksp22I	TGATCA	3 cut(s)	559, 1267, 2062
KspAI	GTTAAC	1 cut(s)	1125
LguI	GCTCTTC	1 cut(s)	1286
LmnI	GCTCC	3 cut(s)	63, 1591, 1949
Lsp1109I	GCAGC	6 cut(s)	41, 350, 1266, 1441, 1954, 2169
LweI	GCATC	6 cut(s)	317, 397, 1291, 1411, 1428, 1480
MaeI	CTAG	8 cut(s)	267, 393, 459, 465, 741, 1214, 1964, 2102
MaeII	ACGT	5 cut(s)	537, 1090, 1186, 1899, 2185
MaeIII	GTNAC	9 cut(s)	545, 953, 1365, 1444, 1505, 1791, 1895, 1934, 2044
MfeI	CAATTG	1 cut(s)	971
MflI	RGATCY	2 cut(s)	622, 1978
MhlI	GDGCHC	2 cut(s)	1519, 1920
MlyI	GAGTC	6 cut(s)	20, 156, 1070, 1229, 1376, 1624
MmeI	TCCRAC	1 cut(s)	2276
Mph1103I	ATGCAT	1 cut(s)	1017
MroXI	GAANNNNTTC	1 cut(s)	1320
MslI	CAYNNNNRTG	1 cut(s)	1740
MspI	CCGG	6 cut(s)	135, 1442, 1725, 1796, 2068, 2237
MspR9I	CCNGG	5 cut(s)	23, 51, 651, 1589, 1725
MunI	CAATTG	1 cut(s)	971
Mva1269I	GAATGC	2 cut(s)	1342, 2160
MvaI	CCWGG	4 cut(s)	23, 51, 651, 1589
MvnI	CGCG	1 cut(s)	1852
MwoI	GCNNNNNNNGC	7 cut(s)	63, 314, 1114, 1260, 1523, 1636, 2179
NciI	CCSGG	1 cut(s)	1725
NlaIV	GGNNCC	2 cut(s)	65, 1980
NmuCI	GTSAC	5 cut(s)	545, 953, 1365, 1505, 1934
NsiI	ATGCAT	1 cut(s)	1017
NspI	RCATGY	3 cut(s)	61, 1019, 1553
PaeI	GCATGC	2 cut(s)	1019, 1553
PaeR7I	CTCGAG	4 cut(s)	28, 720, 2271, 2339
PceI	AGGCCT	2 cut(s)	654, 739
PciSI	GCTCTTC	1 cut(s)	1286
PctI	GAATGC	2 cut(s)	1342, 2160
PdmI	GAANNNNTTC	1 cut(s)	1320
PflFI	GACNNNGTC	1 cut(s)	2198
PflMI	CCANNNNNTGG	1 cut(s)	1975
PfoI	TCCNGGA	1 cut(s)	1723
PkrI	GCNGC	7 cut(s)	56, 365, 1179, 1256, 1456, 1944, 2159
Ple19I	CGATCG	1 cut(s)	2293
PleI	GAGTC	6 cut(s)	20, 155, 1069, 1228, 1376, 1623
PpsI	GAGTC	6 cut(s)	20, 155, 1069, 1228, 1376, 1623
Ppu21I	YACGTR	1 cut(s)	1900
PshAI	GACNNNNGTC	1 cut(s)	395
Psp124BI	GAGCTC	1 cut(s)	1920
Psp6I	CCWGG	4 cut(s)	21, 49, 649, 1587
PspGI	CCWGG	4 cut(s)	21, 49, 649, 1587
PspN4I	GGNNCC	2 cut(s)	65, 1980
PspPI	GGNCC	3 cut(s)	132, 774, 2139
PspXI	VCTCGAGB	2 cut(s)	28, 2339
PstNI	CAGNNNCTG	1 cut(s)	1975
PsuI	RGATCY	2 cut(s)	622, 1978
PsyI	GACNNNGTC	1 cut(s)	2198
PvuI	CGATCG	1 cut(s)	2293
RsaI	GTAC	8 cut(s)	259, 372, 402, 637, 901, 1860, 1958, 2222
RsaNI	GTAC	8 cut(s)	258, 371, 401, 636, 900, 1859, 1957, 2221
RseI	CAYNNNNRTG	1 cut(s)	1740
SacI	GAGCTC	1 cut(s)	1920
SapI	GCTCTTC	1 cut(s)	1286
SatI	GCNGC	7 cut(s)	55, 364, 1178, 1255, 1455, 1943, 2158
Sau96I	GGNCC	3 cut(s)	132, 774, 2139
SchI	GAGTC	6 cut(s)	20, 156, 1070, 1229, 1376, 1624
ScrFI	CCNGG	5 cut(s)	23, 51, 651, 1589, 1725
SduI	GDGCHC	2 cut(s)	1519, 1920
SfaNI	GCATC	6 cut(s)	317, 397, 1291, 1411, 1428, 1480
SfcI	CTRYAG	1 cut(s)	1258
Sfr274I	CTCGAG	4 cut(s)	28, 720, 2271, 2339
SinI	GGWCC	1 cut(s)	774
SlaI	CTCGAG	4 cut(s)	28, 720, 2271, 2339
SmiMI	CAYNNNNRTG	1 cut(s)	1740
SmlI	CTYRAG	7 cut(s)	28, 530, 720, 1913, 2230, 2271, 2339
SmoI	CTYRAG	7 cut(s)	28, 530, 720, 1913, 2230, 2271, 2339
SpeI	ACTAGT	2 cut(s)	392, 458
SphI	GCATGC	2 cut(s)	1019, 1553
SseBI	AGGCCT	2 cut(s)	654, 739
SsiI	CCGC	5 cut(s)	130, 224, 878, 1177, 1457
SspMI	CTAG	8 cut(s)	267, 393, 459, 465, 741, 1214, 1964, 2102
SstI	GAGCTC	1 cut(s)	1920
StuI	AGGCCT	2 cut(s)	654, 739
StyD4I	CCNGG	5 cut(s)	21, 49, 649, 1587, 1723
StyI	CCWWGG	3 cut(s)	329, 958, 2135
TaaI	ACNGT	7 cut(s)	47, 191, 375, 553, 890, 1514, 1541
TaiI	ACGT	5 cut(s)	540, 1093, 1189, 1902, 2188
TaqI	TCGA	8 cut(s)	29, 169, 500, 721, 1289, 1488, 2272, 2340
TatI	WGTACW	4 cut(s)	257, 370, 635, 1858
TauI	GCSGC	1 cut(s)	1180
TseFI	GTSAC	5 cut(s)	545, 953, 1365, 1505, 1934
TseI	GCWGC	6 cut(s)	54, 363, 1254, 1454, 1942, 2157
Tsp45I	GTSAC	5 cut(s)	545, 953, 1365, 1505, 1934
TspDTI	ATGAA	9 cut(s)	471, 527, 780, 872, 1142, 1918, 1981, 2315, 2385
TspGWI	ACGGA	1 cut(s)	367
Tth111I	GACNNNGTC	1 cut(s)	2198
Van91I	CCANNNNNTGG	1 cut(s)	1975
VneI	GTGCAC	1 cut(s)	1515
VpaK11BI	GGWCC	1 cut(s)	774
XagI	CCTNNNNNAGG	1 cut(s)	2102
XapI	RAATTY	3 cut(s)	661, 1750, 1889
XbaI	TCTAGA	1 cut(s)	1213
XceI	RCATGY	3 cut(s)	61, 1019, 1553
XcmI	CCANNNNNNNNNTGG	3 cut(s)	57, 871, 1990
XhoI	CTCGAG	4 cut(s)	28, 720, 2271, 2339
XmnI	GAANNNNTTC	1 cut(s)	1320
XspI	CTAG	8 cut(s)	267, 393, 459, 465, 741, 1214, 1964, 2102
ZraI	GACGTC	1 cut(s)	1187
Zsp2I	ATGCAT	1 cut(s)	1017

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.