Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0008856

Physical Location & Seq

Genomic Coordinates Reverse (-)

20535357 .. 20539010

Gene Structure 3654 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00016570.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2133 bp

ATGAGAAAGCAATTGCAGCGCCAGGACACGGGATTTCCGAGCAGTCATCAAGGCTGCATGTGGAGCCTGATGCATATCCTTGATTACCATCGTTGGCATAGTGTCAAGAAGATACTTCCAGGCCGAAAGCACGGCAGAGGAAGAATCAGAGGTAGTGGAAGTCGAAAAGCAACTTTGAAAAACCACGACAATGGTGAAAAGAAAGAGTTGAGTGCTGAAGCTGAACCCTTGCTGGTCAAACAGAATGGTAAAGAAACGAGCTCTGCAAAGAAAAAGTCTAAGAAATGTCAACTAAAGGCATCAAAAGCTAAACAGGCAATTTCTCCAGAAAAAGTCATGGAGTCCTCACCAAGCAAGAAGCCAATAACAAGTAATCAGCTGAAAAAAGACATTTCCCACGACCAGTTTTATGATCATGTTGATCTTTTGGAGCTAATCAAGGCAAACAAGGAATTTTTCTTAAAATTCCTTCAAGATCCAGATGTTAACAGAAACCAGTTTCCGGGCCTGCACAAGTCCAATACCAAAGTAAGATTAACAAAATCAAGTTCATTTCCTGTAGCTGATTCGTCTAGAGCTAGAAGTGTTATGCCTAGCACACTAAAACACAAGCAGAATGAAATCTGGTCCTTCCCAAAGGGAGAAAAACTTCTTGCTAGTACTCGGGCGCCAAAACTAGTGACATCCGAATCTCAAGAGGACTTGCCTGAGGGATTTAGATATCAAGGGTGGAATCAATTGGTCGTGAGTCGTTTTAAAGATATAAAGCAGAAGCTAATGCATGCACCGCAGGAGAGCAAGAAGGGAAATACTCCCACTTCCACTGAAACACTCATCCCTGAAGACCCTTCCGGATGTGATGGTAAAGAAGTGTGTGAAACACCAGAGATGATCACTGGTGAAGAGAGAAGCAAGATTGATGGTTTGGATGATACTCTTGGAAAGCGAAAACGCCGTGTAAGTAGAACCTCCTCTCTAGATGAGTCACTCAATAGATATAGTCAGTTGTTTGAGAGTAGTTGCAGAAGTGAGGCCAAGTTGCATCACTCCACGAGTTTAAAGTTAAGAAGTGAAGAAAAAGTTACTTCAACTAGGAATGCTCAGAAATCCATCAGAAGAAATCTATCACTACCTGACCTTGACTCTTTGTGTTCCTTTCTAAATGGGCCACCTAGAGATGCTTTTCGCTTAGGGATTCCGGTTAAAAATGTAGTGGACCACAAGACAAGTAAGGAAAATGATAGTCATATTGGTCCCAAGTCGGCGAGCCTTAATGTAGATACAGGTAAGTCGGAAGGACTAGACGCTATTTTAGAGACAGAGTTCCAGAATAGCATAGAAAAACAAAGTGACAGAGAGGACCTTCATGAGTATGTAGTTGAGCCAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAAGCCGAACTCATGAGCAACGAGACATTGGTTTTACGATGAGTCCTGAGGGAGAGAACGCACAACCAAGTGAAAGTTGTACTCTTGAATCTTATTCTTCAGAGTTGTCAATCTCAGAAGAACTCTGCAGCAGTGCAAAGTGTGAGCAAACTGAAATCGAACAGACGAATGTGGATCATTACTCCTCACAATTTGTAGACAAAATGGATGATCCTGAACTCAACTATGTTAGAGATGTTCTTGAGCTGTCAGGTTTCATGGGAAATGAATACCTTGGAACATGGTACTCCATGGACCAGCCTTTGGATCCTTCAGTGTTCAAGGAATTGGAGGCTCGATTTCAGCATCAACTCGATTTTACTGGAAATTGGGAACACCAGCTTCTTTTTGATTTAGTGAATGAAACACTCTTTGATATCCATGAGAGATCATACACCTACTTCCCAAGGGCCTTGTCCTTCAGTGGCTCCAAACACCGTCCAATGCCGAAAGGGCAGCATCTTCTTGAAGATGTGTGGACAAGGATTAGCTCACATATGAGCTTCAGGCCAGAGCTGGTCCAGTCATTGGATGATATCTTGGCTCGAGATTTGGCGAAGGCCGATACTTGGATGAACCTTCAATGGGAAACTGAGTGTGTGGCACTCGAACTTGAGGATTTGATCTTTGAAGAACTATTAGATGAAGTTATCTGTAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3741 DUF4378

Protein Analysis

710

Amino Acids

81.25

Weight (kDa)

5.5

Isoelectric Point (pI)

60.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF3741	PF12552	120 - 161	7.9e-09	Protein of unknown function (DUF3741)
DUF4378	PF14309	550 - 706	2.2e-43	Domain of unknown function (DUF4378)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008856)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g36010 FvH4_3g36010 FvH4_3g36010
malus_domestica	MD11G1109700.v1.1
prunus_persica	Prupe.6G078200_v2.0.a1 Prupe.6G078200_v2.0.a1
pyrus_communis	pycom03g07730 pycom11g09050
rosa_chinensis	RchiOBHm_Chr5g0064421
rosa_laevigata	RLG00000035723
rosa_multiflora	Rmu_sc0001125.1_g000006
rosa_roxburghii	Rroxscaffold_1G00016570
rosa_rugosa	Rorug05G0364600 Rorug05G0364700.1 Rorug05G0364800 Rorug05G0364800 Rorug05G0364800 Rorug05G0364900
rosa_samantha	Rh5AG423200 Rh5BG438700 Rh5CG461400 Rh5DG452000
rosa_wichuraiana	Rw5G039780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	667
AccB7I	CCANNNNNTGG	2 cut(s)	1735, 1999
AccI	GTMKAC	1 cut(s)	1629
AccIII	TCCGGA	1 cut(s)	851
AciI	CCGC	1 cut(s)	788
AclWI	GGATC	5 cut(s)	470, 1614, 1637, 1733, 1746
AcsI	RAATTY	2 cut(s)	452, 464
AcuI	CTGAAG	6 cut(s)	237, 861, 1515, 1728, 1876, 1960
AcyI	GRCGYC	1 cut(s)	668
AfaI	GTAC	3 cut(s)	661, 1513, 1718
AfiI	CCNNNNNNNGG	6 cut(s)	28, 502, 1735, 1999, 2040, 2056
AgsI	TTSAA	8 cut(s)	178, 473, 1089, 1520, 1753, 1940, 2054, 2102
AhlI	ACTAGT	1 cut(s)	676
AjnI	CCWGG	2 cut(s)	21, 118
Alw21I	GWGCWC	1 cut(s)	263
Alw26I	GTCTC	2 cut(s)	1310, 1449
AlwI	GGATC	5 cut(s)	470, 1614, 1637, 1733, 1746
Ama87I	CYCGRG	2 cut(s)	663, 2016
Aor13HI	TCCGGA	1 cut(s)	851
AoxI	GGCC	7 cut(s)	121, 505, 1032, 1166, 1881, 1979, 2031
ApeKI	GCWGC	4 cut(s)	16, 54, 1560, 1927
ApoI	RAATTY	2 cut(s)	452, 464
ArsI	GACNNNNNNTTYG	2 cut(s)	260, 292
Asp700I	GAANNNNTTC	1 cut(s)	648
AspLEI	GCGC	2 cut(s)	21, 670
AspS9I	GGNCC	9 cut(s)	505, 627, 1166, 1216, 1253, 1360, 1726, 1881, 1990
AsuC2I	CCSGG	1 cut(s)	504
AsuHPI	GGTGA	3 cut(s)	206, 339, 911
AvaI	CYCGRG	2 cut(s)	663, 2016
AvaII	GGWCC	6 cut(s)	627, 1216, 1253, 1360, 1726, 1990
AxyI	CCTNAGG	2 cut(s)	708, 1479
BamHI	GGATCC	1 cut(s)	1738
BanI	GGYRCC	1 cut(s)	667
BanII	GRGCYC	1 cut(s)	263
BauI	CACGAG	1 cut(s)	1051
BbsI	GAAGAC	1 cut(s)	849
Bbv12I	GWGCWC	1 cut(s)	263
BbvI	GCAGC	4 cut(s)	28, 41, 1572, 1939
BccI	CCATC	4 cut(s)	96, 854, 914, 1118
BceAI	ACGGC	2 cut(s)	148, 939
BciT130I	CCWGG	2 cut(s)	23, 120
BclI	TGATCA	2 cut(s)	412, 891
BcnI	CCSGG	1 cut(s)	504
BcoDI	GTCTC	2 cut(s)	1310, 1449
BcuI	ACTAGT	1 cut(s)	676
BfaI	CTAG	9 cut(s)	573, 579, 594, 657, 677, 977, 1092, 1173, 1301
BfmI	CTRYAG	3 cut(s)	558, 1558, 2125
BfoI	RGCGCY	2 cut(s)	22, 671
BglI	GCCNNNNNGGC	1 cut(s)	1924
BisI	GCNGC	4 cut(s)	17, 55, 1561, 1928
BlsI	GCNGC	4 cut(s)	18, 56, 1562, 1929
BmcAI	AGTACT	1 cut(s)	661
Bme1390I	CCNGG	3 cut(s)	23, 120, 504
Bme18I	GGWCC	6 cut(s)	627, 1216, 1253, 1360, 1726, 1990
BmeT110I	CYCGRG	2 cut(s)	663, 2016
BmgT120I	GGNCC	9 cut(s)	505, 627, 1166, 1216, 1253, 1360, 1726, 1881, 1990
BmiI	GGNNCC	5 cut(s)	65, 669, 1255, 1740, 1900
BmrFI	CCNGG	3 cut(s)	23, 120, 504
BmsI	GCATC	6 cut(s)	60, 308, 1051, 1168, 1786, 1939
BpiI	GAAGAC	1 cut(s)	849
BpmI	CTGGAG	1 cut(s)	309
Bpu10I	CCTNAGC	1 cut(s)	1189
BpuEI	CTTGAG	3 cut(s)	678, 1694, 2105
BpuMI	CCSGG	1 cut(s)	504
BsaBI	GATNNNNATC	2 cut(s)	74, 87
BsaHI	GRCGYC	1 cut(s)	668
BsaJI	CCNNGG	3 cut(s)	1705, 1722, 1877
BsaWI	WCCGGW	2 cut(s)	851, 1198
Bsc4I	CCNNNNNNNGG	6 cut(s)	28, 502, 1735, 1999, 2040, 2056
Bse1I	ACTGG	5 cut(s)	403, 496, 901, 1798, 1993
Bse21I	CCTNAGG	2 cut(s)	708, 1479
Bse8I	GATNNNNATC	2 cut(s)	74, 87
BseAI	TCCGGA	1 cut(s)	851
BseBI	CCWGG	2 cut(s)	23, 120
BseDI	CCNNGG	3 cut(s)	1705, 1722, 1877
BseGI	GGATG	7 cut(s)	683, 834, 860, 934, 1645, 2008, 2049
BseJI	GATNNNNATC	2 cut(s)	74, 87
BseLI	CCNNNNNNNGG	6 cut(s)	28, 502, 1735, 1999, 2040, 2056
BseMII	CTCAG	5 cut(s)	699, 1115, 1470, 1560, 2055
BseNI	ACTGG	5 cut(s)	403, 496, 901, 1798, 1993
BseRI	GAGGAG	2 cut(s)	961, 1606
BseXI	GCAGC	4 cut(s)	28, 41, 1572, 1939
BsgI	GTGCAG	1 cut(s)	494
BshFI	GGCC	7 cut(s)	123, 507, 1034, 1168, 1883, 1981, 2033
BshNI	GGYRCC	1 cut(s)	667
BsiHKAI	GWGCWC	1 cut(s)	263
BsiHKCI	CYCGRG	2 cut(s)	663, 2016
BsiSI	CCGG	3 cut(s)	503, 852, 1199
BslFI	GGGAC	1 cut(s)	1239
BslI	CCNNNNNNNGG	6 cut(s)	28, 502, 1735, 1999, 2040, 2056
BsmAI	GTCTC	2 cut(s)	1310, 1449
BsmFI	GGGAC	1 cut(s)	1239
BsmI	GAATGC	1 cut(s)	1102
BsnI	GGCC	7 cut(s)	123, 507, 1034, 1168, 1883, 1981, 2033
BsoBI	CYCGRG	2 cut(s)	663, 2016
Bsp1286I	GDGCHC	1 cut(s)	263
Bsp13I	TCCGGA	1 cut(s)	851
Bsp143I	GATC	9 cut(s)	412, 421, 475, 891, 1606, 1642, 1738, 1859, 2094
Bsp19I	CCATGG	1 cut(s)	1722
BspACI	CCGC	1 cut(s)	788
BspANI	GGCC	7 cut(s)	123, 507, 1034, 1168, 1883, 1981, 2033
BspCNI	CTCAG	5 cut(s)	700, 1114, 1471, 1559, 2056
BspEI	TCCGGA	1 cut(s)	851
BspHI	TCATGA	2 cut(s)	1366, 1444
BspLI	GGNNCC	5 cut(s)	65, 669, 1255, 1740, 1900
BspMAI	CTGCAG	1 cut(s)	1562
BspPI	GGATC	5 cut(s)	470, 1614, 1637, 1733, 1746
BspT107I	GGYRCC	1 cut(s)	667
BsrI	ACTGG	5 cut(s)	403, 496, 901, 1798, 1993
BssECI	CCNNGG	3 cut(s)	1705, 1722, 1877
BssMI	GATC	9 cut(s)	412, 421, 475, 891, 1606, 1642, 1738, 1859, 2094
BssNI	GRCGYC	1 cut(s)	668
BssSI	CACGAG	1 cut(s)	1051
BssT1I	CCWWGG	3 cut(s)	1705, 1722, 1877
Bst2BI	CACGAG	1 cut(s)	1051
Bst2UI	CCWGG	2 cut(s)	23, 120
Bst4CI	ACNGT	1 cut(s)	1910
Bst6I	CTCTTC	1 cut(s)	897
BstACI	GRCGYC	1 cut(s)	668
BstC8I	GCNNGC	3 cut(s)	509, 783, 1267
BstDEI	CTNAG	7 cut(s)	279, 708, 1101, 1189, 1479, 1546, 2064
BstDSI	CCRYGG	1 cut(s)	1722
BstF5I	GGATG	7 cut(s)	683, 834, 860, 934, 1645, 2008, 2049
BstH2I	RGCGCY	2 cut(s)	22, 671
BstHHI	GCGC	2 cut(s)	21, 670
BstKTI	GATC	9 cut(s)	415, 424, 478, 894, 1609, 1645, 1741, 1862, 2097
BstMAI	GTCTC	2 cut(s)	1310, 1449
BstMBI	GATC	9 cut(s)	412, 421, 475, 891, 1606, 1642, 1738, 1859, 2094
BstMWI	GCNNNNNNNGC	6 cut(s)	16, 63, 305, 314, 787, 1924
BstNI	CCWGG	2 cut(s)	23, 120
BstNSI	RCATGY	2 cut(s)	61, 785
BstSCI	CCNGG	3 cut(s)	21, 118, 502
BstSFI	CTRYAG	3 cut(s)	558, 1558, 2125
BstV1I	GCAGC	4 cut(s)	28, 41, 1572, 1939
BstV2I	GAAGAC	1 cut(s)	849
BstX2I	RGATCY	2 cut(s)	475, 1738
BstXI	CCANNNNNNTGG	1 cut(s)	191
BstYI	RGATCY	2 cut(s)	475, 1738
Bsu36I	CCTNAGG	2 cut(s)	708, 1479
BsuRI	GGCC	7 cut(s)	123, 507, 1034, 1168, 1883, 1981, 2033
BtgI	CCRYGG	1 cut(s)	1722
BtsCI	GGATG	7 cut(s)	683, 834, 860, 934, 1645, 2008, 2049
BtsI	GCAGTG	1 cut(s)	1570
BtsIMutI	CAGTG	5 cut(s)	822, 894, 1570, 1752, 1900
Cac8I	GCNNGC	3 cut(s)	509, 783, 1267
CciI	TCATGA	2 cut(s)	1366, 1444
CfoI	GCGC	2 cut(s)	21, 670
Cfr13I	GGNCC	9 cut(s)	505, 627, 1166, 1216, 1253, 1360, 1726, 1881, 1990
CseI	GACGC	1 cut(s)	1313
Csp6I	GTAC	3 cut(s)	660, 1512, 1717
CviQI	GTAC	3 cut(s)	660, 1512, 1717
DdeI	CTNAG	7 cut(s)	279, 708, 1101, 1189, 1479, 1546, 2064
DinI	GGCGCC	1 cut(s)	669
DpnI	GATC	9 cut(s)	414, 423, 477, 893, 1608, 1644, 1740, 1861, 2096
DpnII	GATC	9 cut(s)	412, 421, 475, 891, 1606, 1642, 1738, 1859, 2094
DraI	TTTAAA	2 cut(s)	757, 1059
Eam1104I	CTCTTC	1 cut(s)	897
EarI	CTCTTC	1 cut(s)	897
Ecl136II	GAGCTC	1 cut(s)	261
Eco130I	CCWWGG	3 cut(s)	1705, 1722, 1877
Eco24I	GRGCYC	1 cut(s)	263
Eco32I	GATATC	3 cut(s)	722, 1849, 2008
Eco47I	GGWCC	6 cut(s)	627, 1216, 1253, 1360, 1726, 1990
Eco53kI	GAGCTC	1 cut(s)	261
Eco57I	CTGAAG	6 cut(s)	237, 861, 1515, 1728, 1876, 1960
Eco81I	CCTNAGG	2 cut(s)	708, 1479
Eco88I	CYCGRG	2 cut(s)	663, 2016
EcoICRI	GAGCTC	1 cut(s)	261
EcoO109I	RGGNCCY	2 cut(s)	1360, 1881
EcoRII	CCWGG	2 cut(s)	21, 118
EcoRV	GATATC	3 cut(s)	722, 1849, 2008
EcoT14I	CCWWGG	3 cut(s)	1705, 1722, 1877
EcoT22I	ATGCAT	2 cut(s)	75, 783
EcoT38I	GRGCYC	1 cut(s)	263
EgeI	GGCGCC	1 cut(s)	669
EheI	GGCGCC	1 cut(s)	669
ErhI	CCWWGG	3 cut(s)	1705, 1722, 1877
FaqI	GGGAC	1 cut(s)	1239
FauNDI	CATATG	1 cut(s)	1968
FbaI	TGATCA	2 cut(s)	412, 891
FblI	GTMKAC	1 cut(s)	1629
Fnu4HI	GCNGC	4 cut(s)	17, 55, 1561, 1928
FokI	GGATG	7 cut(s)	670, 821, 867, 941, 1652, 2015, 2056
FriOI	GRGCYC	1 cut(s)	263
Fsp4HI	GCNGC	4 cut(s)	17, 55, 1561, 1928
FspBI	CTAG	9 cut(s)	573, 579, 594, 657, 677, 977, 1092, 1173, 1301
GlaI	GCGC	2 cut(s)	20, 669
GluI	GCNGC	4 cut(s)	17, 55, 1561, 1928
GsuI	CTGGAG	1 cut(s)	309
HaeII	RGCGCY	2 cut(s)	22, 671
HaeIII	GGCC	7 cut(s)	123, 507, 1034, 1168, 1883, 1981, 2033
HapII	CCGG	3 cut(s)	503, 852, 1199
HgaI	GACGC	1 cut(s)	1313
HhaI	GCGC	2 cut(s)	21, 670
Hin1I	GRCGYC	1 cut(s)	668
Hin6I	GCGC	2 cut(s)	19, 668
HinP1I	GCGC	2 cut(s)	19, 668
HincII	GTYRAC	2 cut(s)	290, 487
HindII	GTYRAC	2 cut(s)	290, 487
HpaI	GTTAAC	1 cut(s)	487
HpaII	CCGG	3 cut(s)	503, 852, 1199
HphI	GGTGA	3 cut(s)	206, 339, 911
Hpy166II	GTNNAC	5 cut(s)	290, 487, 1216, 1630, 1950
Hpy188I	TCNGA	8 cut(s)	39, 149, 688, 1104, 1115, 1294, 1534, 1549
Hpy8I	GTNNAC	5 cut(s)	290, 487, 1216, 1630, 1950
HpyCH4III	ACNGT	1 cut(s)	1910
HpyF10VI	GCNNNNNNNGC	6 cut(s)	16, 63, 305, 314, 787, 1924
HpyF3I	CTNAG	7 cut(s)	279, 708, 1101, 1189, 1479, 1546, 2064
Hsp92I	GRCGYC	1 cut(s)	668
HspAI	GCGC	2 cut(s)	19, 668
KasI	GGCGCC	1 cut(s)	667
Kpn2I	TCCGGA	1 cut(s)	851
Ksp22I	TGATCA	2 cut(s)	412, 891
KspAI	GTTAAC	1 cut(s)	487
Kzo9I	GATC	9 cut(s)	412, 421, 475, 891, 1606, 1642, 1738, 1859, 2094
LmnI	GCTCC	3 cut(s)	63, 430, 1904
Lsp1109I	GCAGC	4 cut(s)	28, 41, 1572, 1939
LweI	GCATC	6 cut(s)	60, 308, 1051, 1168, 1786, 1939
MaeI	CTAG	9 cut(s)	573, 579, 594, 657, 677, 977, 1092, 1173, 1301
MaeIII	GTNAC	4 cut(s)	679, 984, 1081, 1349
MalI	GATC	9 cut(s)	414, 423, 477, 893, 1608, 1644, 1740, 1861, 2096
MboI	GATC	9 cut(s)	412, 421, 475, 891, 1606, 1642, 1738, 1859, 2094
MfeI	CAATTG	2 cut(s)	11, 737
MflI	RGATCY	2 cut(s)	475, 1738
MhlI	GDGCHC	1 cut(s)	263
MluCI	AATT	8 cut(s)	11, 318, 452, 464, 737, 1622, 1757, 1798
Mly113I	GGCGCC	1 cut(s)	668
MlyI	GAGTC	5 cut(s)	350, 757, 992, 1136, 1483
MmeI	TCCRAC	1 cut(s)	1272
Mph1103I	ATGCAT	2 cut(s)	75, 783
MroI	TCCGGA	1 cut(s)	851
MroXI	GAANNNNTTC	1 cut(s)	648
MseI	TTAA	8 cut(s)	461, 486, 536, 756, 1058, 1064, 1203, 1272
MslI	CAYNNNNRTG	2 cut(s)	189, 1371
MspA1I	CMGCKG	1 cut(s)	379
MspI	CCGG	3 cut(s)	503, 852, 1199
MspR9I	CCNGG	3 cut(s)	23, 120, 504
MunI	CAATTG	2 cut(s)	11, 737
Mva1269I	GAATGC	1 cut(s)	1102
MvaI	CCWGG	2 cut(s)	23, 120
MwoI	GCNNNNNNNGC	6 cut(s)	16, 63, 305, 314, 787, 1924
NarI	GGCGCC	1 cut(s)	668
NciI	CCSGG	1 cut(s)	504
NcoI	CCATGG	1 cut(s)	1722
NdeI	CATATG	1 cut(s)	1968
NdeII	GATC	9 cut(s)	412, 421, 475, 891, 1606, 1642, 1738, 1859, 2094
NlaIV	GGNNCC	5 cut(s)	65, 669, 1255, 1740, 1900
NmuCI	GTSAC	3 cut(s)	679, 984, 1349
NsiI	ATGCAT	2 cut(s)	75, 783
NspI	RCATGY	2 cut(s)	61, 785
PaeI	GCATGC	1 cut(s)	785
PaeR7I	CTCGAG	1 cut(s)	2016
PagI	TCATGA	2 cut(s)	1366, 1444
PcsI	WCGNNNNNNNCGW	1 cut(s)	35
PctI	GAATGC	1 cut(s)	1102
PdmI	GAANNNNTTC	1 cut(s)	648
PfeI	GAWTC	6 cut(s)	144, 566, 689, 733, 1195, 1520
PflMI	CCANNNNNTGG	2 cut(s)	1735, 1999
PkrI	GCNGC	4 cut(s)	18, 56, 1562, 1929
PleI	GAGTC	5 cut(s)	349, 756, 991, 1136, 1482
PluTI	GGCGCC	1 cut(s)	671
PpsI	GAGTC	5 cut(s)	349, 756, 991, 1136, 1482
PpuMI	RGGWCCY	1 cut(s)	1360
Psp124BI	GAGCTC	1 cut(s)	263
Psp5II	RGGWCCY	1 cut(s)	1360
Psp6I	CCWGG	2 cut(s)	21, 118
PspGI	CCWGG	2 cut(s)	21, 118
PspN4I	GGNNCC	5 cut(s)	65, 669, 1255, 1740, 1900
PspPI	GGNCC	9 cut(s)	505, 627, 1166, 1216, 1253, 1360, 1726, 1881, 1990
PspPPI	RGGWCCY	1 cut(s)	1360
PstI	CTGCAG	1 cut(s)	1562
PsuI	RGATCY	2 cut(s)	475, 1738
PvuII	CAGCTG	1 cut(s)	379
RsaI	GTAC	3 cut(s)	661, 1513, 1718
RsaNI	GTAC	3 cut(s)	660, 1512, 1717
RseI	CAYNNNNRTG	2 cut(s)	189, 1371
SacI	GAGCTC	1 cut(s)	263
SaqAI	TTAA	8 cut(s)	461, 486, 536, 756, 1058, 1064, 1203, 1272
SatI	GCNGC	4 cut(s)	17, 55, 1561, 1928
Sau3AI	GATC	9 cut(s)	412, 421, 475, 891, 1606, 1642, 1738, 1859, 2094
Sau96I	GGNCC	9 cut(s)	505, 627, 1166, 1216, 1253, 1360, 1726, 1881, 1990
ScaI	AGTACT	1 cut(s)	661
SchI	GAGTC	5 cut(s)	350, 757, 992, 1136, 1483
ScrFI	CCNGG	3 cut(s)	23, 120, 504
SduI	GDGCHC	1 cut(s)	263
SfaNI	GCATC	6 cut(s)	60, 308, 1051, 1168, 1786, 1939
SfcI	CTRYAG	3 cut(s)	558, 1558, 2125
SfoI	GGCGCC	1 cut(s)	669
Sfr274I	CTCGAG	1 cut(s)	2016
SinI	GGWCC	6 cut(s)	627, 1216, 1253, 1360, 1726, 1990
SlaI	CTCGAG	1 cut(s)	2016
SmiMI	CAYNNNNRTG	2 cut(s)	189, 1371
SmlI	CTYRAG	4 cut(s)	693, 1673, 2016, 2084
SmoI	CTYRAG	4 cut(s)	693, 1673, 2016, 2084
SpeI	ACTAGT	1 cut(s)	676
SphI	GCATGC	1 cut(s)	785
Sse9I	AATT	8 cut(s)	11, 318, 452, 464, 737, 1622, 1757, 1798
SsiI	CCGC	1 cut(s)	788
SspDI	GGCGCC	1 cut(s)	667
SspMI	CTAG	9 cut(s)	573, 579, 594, 657, 677, 977, 1092, 1173, 1301
SstI	GAGCTC	1 cut(s)	263
StyD4I	CCNGG	3 cut(s)	21, 118, 502
StyI	CCWWGG	3 cut(s)	1705, 1722, 1877
TaaI	ACNGT	1 cut(s)	1910
TaqI	TCGA	6 cut(s)	163, 1590, 1768, 1785, 2017, 2079
TasI	AATT	8 cut(s)	11, 318, 452, 464, 737, 1622, 1757, 1798
TatI	WGTACW	2 cut(s)	659, 1511
TfiI	GAWTC	6 cut(s)	144, 566, 689, 733, 1195, 1520
Tru1I	TTAA	8 cut(s)	461, 486, 536, 756, 1058, 1064, 1203, 1272
Tru9I	TTAA	8 cut(s)	461, 486, 536, 756, 1058, 1064, 1203, 1272
TscAI	CASTG	5 cut(s)	829, 901, 1570, 1752, 1900
TseFI	GTSAC	3 cut(s)	679, 984, 1349
TseI	GCWGC	4 cut(s)	16, 54, 1560, 1927
Tsp45I	GTSAC	3 cut(s)	679, 984, 1349
TspDTI	ATGAA	8 cut(s)	540, 633, 1355, 1678, 1713, 1848, 2060, 2130
TspRI	CASTG	5 cut(s)	829, 901, 1570, 1752, 1900
Van91I	CCANNNNNTGG	2 cut(s)	1735, 1999
VpaK11BI	GGWCC	6 cut(s)	627, 1216, 1253, 1360, 1726, 1990
XapI	RAATTY	2 cut(s)	452, 464
XbaI	TCTAGA	2 cut(s)	572, 976
XceI	RCATGY	2 cut(s)	61, 785
XhoI	CTCGAG	1 cut(s)	2016
XmiI	GTMKAC	1 cut(s)	1629
XmnI	GAANNNNTTC	1 cut(s)	648
XspI	CTAG	9 cut(s)	573, 579, 594, 657, 677, 977, 1092, 1173, 1301
ZrmI	AGTACT	1 cut(s)	661
Zsp2I	ATGCAT	2 cut(s)	75, 783

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.