Rh5BG438700

Domain of unknown function (DUF4378)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0008856
Physical Location & Seq
Forward (+)
70680701 .. 70684682
3982 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG438700.1

Sequence Viewer

Length: 1749 bp
ATGAGAAAGCAATTGCAGCGCCAGGACACGGGATTTCCGAGCAGTCATCAAGGCTGCATGTGGAGCCTGATGCATATCCTTGATTACCATCGTTGGCATAGTGTCAAGAAGATGCTTCCAGGCCGAAAGCACGGCAGAGGAAGAATCAGAGGTAGTGGAAGTCGAAAAGCAACTTTGAAAAACCACGACAATGGTGAAAAGAAAGAGTTGAGTGCTGAAGCTGAACCCTTGCAGGTCAAACAGAATGGTAAAGAAACGAGCTCTGCAAGGAAGAAGTCCAAGAAATCTCAACTAAAGGCATCAAAAGCTAAACAGGTATGTAAAGAAGAAAGCCACAAAAGATGGATTGTAAGCTTCTCTGGGCAACCATGGTTGTGGAGAACTAGTGAAATGCATGATGTCAAGCCTTCAGATGATTTTTTGAAGGTGCCTTATGAGTTGGTTGCATCTAATGAAAACTGTACCCTGGAACAGGCAATTTCTCCAGAAAAAGTCATGGAGTCCTCACCAAGCAAGAAGCCAATAACAAGTAATCAGCTGAAAAAAGACATTTCCCACGACCAGTTTTATGATCATGTTGATCTTTTGGAGCTAATCAAGGAAAACAAGGAATTTTTCTTTAAATTCCTTCAAGATCCAGATGTTAACAGAAACCAGTTTCCGGGCCTGCACAAGTCCAATACCAAAGTAAGATTAACAAAATCAAGTTCATTTCCTGTAGCTGATTCGTCTAGAGCTATAAGTGTTATGCCTAGCACACTAAAACACAAGCAGAATGAAATCTGGTCCTTCCCAAAGGGAGAAAAACTTCTTGCTAGTACTCGGTCGCCAAAATTAGTGACATTCGAATCTCAAGAGGACCTGCCTGAGGGATTTAGATATCAAGGGTGGAATCAATTGGTCGTGAGTCGTTTTAAAGATATAAAGCAGAAGCTAATGCATGCACCGCAGGAGAGCAAGAAGGGAAATACTCCCACTTCCACTGAAACACTCATCCCTGAAGACCCTTCTGGATGTGATGGTAATGAAATGTATGAAACACCAGAGATGATCACTGGTGAAGAGAGAAGCAAGATTGATGGTTTGGATGATACTCTTGGAAAGCGAAAACGCCGTGTAAGTAGAACCTCCTCTCTAGATGAGTCACTCAATAGATATAGTCAGTTGTTTGAGAGTAGTTGCAGAAGTGAGGCCAAGTTGCATCACTCCATGAGTTTAAAGTTAAGAAGTGAAGAAAAAGTTACTTCAACTGGGAATGCTCAGAAATCCACCAGAAGAAATCTGTCACTACCTGACCTTGACTCTTTGTGTTCCTTTCTAAATGGGCCACCTAGAGATGCTTTTCGCTTAGGGATTCCGGTTAAAAATGTAGTGGACCACAAGACAAGTAAGGAAAATGATAGTCATATTGGTCCCAAGTCGGCGAGCCTTAATGTAGATACAGGTAAGTCTGAAGGACTAGAAGCTATTTTAGAGACAGAGTTCCAGAATGGCATAGAAGAACAAAGTGACAGAGAGGACCTTCATGAGTATGTAGTTGAGCCAGAAGAAGAAGAAGAAGAAAGCCGAACTCATGAGCAACGAAACATTGGTTTTACGATGAGTCCTGAGGGAGAGAACGCACAACCAAGTGAAAGTTGTACTCTTGAATCTTATTCTTCAGAGTTGTCAATCTCAGAAGGTAATACTGTTCATACATGCCTATTGTCACTGCACATTTATTTTACATGTCTCAAGTATTTTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3741 DUF4378

Protein Analysis

582

Amino Acids

66.28

Weight (kDa)

7.65

Isoelectric Point (pI)

64.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3741 PF12552 173 - 214 7.8e-07 Protein of unknown function (DUF3741)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 223, 870
AccB1I GGYRCC 1 cut(s) 427
AciI CCGC 1 cut(s) 947
AclWI GGATC 1 cut(s) 629
AcsI RAATTY 2 cut(s) 611, 623
AcuI CTGAAG 5 cut(s) 237, 393, 1020, 1473, 1644
AfaI GTAC 3 cut(s) 463, 820, 1642
AfiI CCNNNNNNNGG 4 cut(s) 28, 472, 661, 868
AflIII ACRYGT 1 cut(s) 1727
AgsI TTSAA 5 cut(s) 178, 424, 632, 1248, 1649
AhlI ACTAGT 1 cut(s) 383
AjnI CCWGG 3 cut(s) 21, 118, 465
Alw21I GWGCWC 1 cut(s) 263
Alw26I GTCTC 2 cut(s) 1469, 1736
AlwI GGATC 1 cut(s) 629
AoxI GGCC 4 cut(s) 121, 664, 1191, 1325
ApeKI GCWGC 2 cut(s) 16, 54
ApoI RAATTY 2 cut(s) 611, 623
Asp700I GAANNNNTTC 1 cut(s) 807
AspLEI GCGC 1 cut(s) 21
AspS9I GGNCC 7 cut(s) 664, 786, 859, 1325, 1375, 1412, 1519
AsuC2I CCSGG 1 cut(s) 663
AsuHPI GGTGA 3 cut(s) 206, 498, 1070
AsuII TTCGAA 1 cut(s) 846
AvaII GGWCC 5 cut(s) 786, 859, 1375, 1412, 1519
AxyI CCTNAGG 2 cut(s) 867, 1608
BanI GGYRCC 1 cut(s) 427
BanII GRGCYC 1 cut(s) 263
BbsI GAAGAC 1 cut(s) 1008
Bbv12I GWGCWC 1 cut(s) 263
BbvI GCAGC 2 cut(s) 28, 41
BccI CCATC 4 cut(s) 96, 336, 1013, 1073
BceAI ACGGC 2 cut(s) 148, 1098
BciT130I CCWGG 3 cut(s) 23, 120, 467
BclI TGATCA 2 cut(s) 571, 1050
BcnI CCSGG 1 cut(s) 663
BcoDI GTCTC 2 cut(s) 1469, 1736
BcuI ACTAGT 1 cut(s) 383
BfaI CTAG 7 cut(s) 384, 732, 753, 816, 1136, 1332, 1460
BfmI CTRYAG 1 cut(s) 717
BfoI RGCGCY 1 cut(s) 22
BfuAI ACCTGC 2 cut(s) 223, 870
BisI GCNGC 2 cut(s) 17, 55
BlsI GCNGC 2 cut(s) 18, 56
BmcAI AGTACT 1 cut(s) 820
Bme1390I CCNGG 4 cut(s) 23, 120, 467, 663
Bme18I GGWCC 5 cut(s) 786, 859, 1375, 1412, 1519
BmgT120I GGNCC 7 cut(s) 664, 786, 859, 1325, 1375, 1412, 1519
BmiI GGNNCC 3 cut(s) 65, 429, 1414
BmrFI CCNGG 4 cut(s) 23, 120, 467, 663
BmrI ACTGGG 1 cut(s) 1260
BmsI GCATC 6 cut(s) 60, 102, 308, 455, 1210, 1327
BmuI ACTGGG 1 cut(s) 1260
BpiI GAAGAC 1 cut(s) 1008
BpmI CTGGAG 1 cut(s) 468
Bpu10I CCTNAGC 1 cut(s) 1348
Bpu14I TTCGAA 1 cut(s) 846
BpuEI CTTGAG 2 cut(s) 837, 1718
BpuMI CCSGG 1 cut(s) 663
BsaBI GATNNNNATC 2 cut(s) 74, 87
BsaJI CCNNGG 2 cut(s) 368, 465
BsaWI WCCGGW 1 cut(s) 1357
BsaXI ACNNNNNCTCC 2 cut(s) 370, 400
Bsc4I CCNNNNNNNGG 4 cut(s) 28, 472, 661, 868
Bse1I ACTGG 4 cut(s) 562, 655, 1060, 1255
Bse21I CCTNAGG 2 cut(s) 867, 1608
Bse8I GATNNNNATC 2 cut(s) 74, 87
BseBI CCWGG 3 cut(s) 23, 120, 467
BseDI CCNNGG 2 cut(s) 368, 465
BseGI GGATG 3 cut(s) 993, 1019, 1093
BseJI GATNNNNATC 2 cut(s) 74, 87
BseLI CCNNNNNNNGG 4 cut(s) 28, 472, 661, 868
BseMII CTCAG 4 cut(s) 858, 1274, 1599, 1689
BseNI ACTGG 4 cut(s) 562, 655, 1060, 1255
BseRI GAGGAG 1 cut(s) 1120
BseXI GCAGC 2 cut(s) 28, 41
BsgI GTGCAG 2 cut(s) 653, 1697
Bsh1285I CGRYCG 1 cut(s) 827
BshFI GGCC 4 cut(s) 123, 666, 1193, 1327
BshNI GGYRCC 1 cut(s) 427
BsiEI CGRYCG 1 cut(s) 827
BsiHKAI GWGCWC 1 cut(s) 263
BsiSI CCGG 2 cut(s) 662, 1358
BslFI GGGAC 1 cut(s) 1398
BslI CCNNNNNNNGG 4 cut(s) 28, 472, 661, 868
BsmAI GTCTC 2 cut(s) 1469, 1736
BsmFI GGGAC 1 cut(s) 1398
BsmI GAATGC 1 cut(s) 1261
BsnI GGCC 4 cut(s) 123, 666, 1193, 1327
Bsp119I TTCGAA 1 cut(s) 846
Bsp1286I GDGCHC 1 cut(s) 263
Bsp143I GATC 4 cut(s) 571, 580, 634, 1050
Bsp19I CCATGG 1 cut(s) 368
BspACI CCGC 1 cut(s) 947
BspANI GGCC 4 cut(s) 123, 666, 1193, 1327
BspCNI CTCAG 4 cut(s) 859, 1273, 1600, 1688
BspHI TCATGA 2 cut(s) 1525, 1573
BspLI GGNNCC 3 cut(s) 65, 429, 1414
BspMI ACCTGC 2 cut(s) 223, 870
BspPI GGATC 1 cut(s) 629
BspT104I TTCGAA 1 cut(s) 846
BspT107I GGYRCC 1 cut(s) 427
BsrI ACTGG 4 cut(s) 562, 655, 1060, 1255
BssECI CCNNGG 2 cut(s) 368, 465
BssMI GATC 4 cut(s) 571, 580, 634, 1050
BssT1I CCWWGG 1 cut(s) 368
Bst2UI CCWGG 3 cut(s) 23, 120, 467
Bst4CI ACNGT 2 cut(s) 461, 1690
Bst6I CTCTTC 1 cut(s) 1056
BstBI TTCGAA 1 cut(s) 846
BstC8I GCNNGC 3 cut(s) 668, 942, 1426
BstDEI CTNAG 5 cut(s) 867, 1260, 1348, 1608, 1675
BstDSI CCRYGG 1 cut(s) 368
BstENI CCTNNNNNAGG 2 cut(s) 470, 866
BstF5I GGATG 3 cut(s) 993, 1019, 1093
BstH2I RGCGCY 1 cut(s) 22
BstHHI GCGC 1 cut(s) 21
BstKTI GATC 4 cut(s) 574, 583, 637, 1053
BstMAI GTCTC 2 cut(s) 1469, 1736
BstMBI GATC 4 cut(s) 571, 580, 634, 1050
BstMCI CGRYCG 1 cut(s) 827
BstMWI GCNNNNNNNGC 4 cut(s) 16, 63, 305, 946
BstNI CCWGG 3 cut(s) 23, 120, 467
BstNSI RCATGY 4 cut(s) 61, 944, 1701, 1731
BstSCI CCNGG 4 cut(s) 21, 118, 465, 661
BstSFI CTRYAG 1 cut(s) 717
BstV1I GCAGC 2 cut(s) 28, 41
BstV2I GAAGAC 1 cut(s) 1008
BstX2I RGATCY 1 cut(s) 634
BstXI CCANNNNNNTGG 2 cut(s) 191, 375
BstYI RGATCY 1 cut(s) 634
Bsu36I CCTNAGG 2 cut(s) 867, 1608
BsuRI GGCC 4 cut(s) 123, 666, 1193, 1327
BtgI CCRYGG 1 cut(s) 368
BtsCI GGATG 3 cut(s) 993, 1019, 1093
BtsI GCAGTG 1 cut(s) 1709
BtsIMutI CAGTG 3 cut(s) 981, 1053, 1709
BveI ACCTGC 2 cut(s) 223, 870
Cac8I GCNNGC 3 cut(s) 668, 942, 1426
CciI TCATGA 2 cut(s) 1525, 1573
CfoI GCGC 1 cut(s) 21
Cfr13I GGNCC 7 cut(s) 664, 786, 859, 1325, 1375, 1412, 1519
Csp6I GTAC 3 cut(s) 462, 819, 1641
CviQI GTAC 3 cut(s) 462, 819, 1641
DdeI CTNAG 5 cut(s) 867, 1260, 1348, 1608, 1675
DpnI GATC 4 cut(s) 573, 582, 636, 1052
DpnII GATC 4 cut(s) 571, 580, 634, 1050
DraI TTTAAA 3 cut(s) 622, 916, 1218
Eam1104I CTCTTC 1 cut(s) 1056
EarI CTCTTC 1 cut(s) 1056
Ecl136II GAGCTC 1 cut(s) 261
Eco130I CCWWGG 1 cut(s) 368
Eco24I GRGCYC 1 cut(s) 263
Eco32I GATATC 1 cut(s) 881
Eco47I GGWCC 5 cut(s) 786, 859, 1375, 1412, 1519
Eco53kI GAGCTC 1 cut(s) 261
Eco57I CTGAAG 5 cut(s) 237, 393, 1020, 1473, 1644
Eco81I CCTNAGG 2 cut(s) 867, 1608
EcoICRI GAGCTC 1 cut(s) 261
EcoNI CCTNNNNNAGG 2 cut(s) 470, 866
EcoO109I RGGNCCY 2 cut(s) 859, 1519
EcoRII CCWGG 3 cut(s) 21, 118, 465
EcoRV GATATC 1 cut(s) 881
EcoT14I CCWWGG 1 cut(s) 368
EcoT22I ATGCAT 3 cut(s) 75, 396, 942
EcoT38I GRGCYC 1 cut(s) 263
ErhI CCWWGG 1 cut(s) 368
FaqI GGGAC 1 cut(s) 1398
FbaI TGATCA 2 cut(s) 571, 1050
Fnu4HI GCNGC 2 cut(s) 17, 55
FokI GGATG 3 cut(s) 980, 1026, 1100
FriOI GRGCYC 1 cut(s) 263
Fsp4HI GCNGC 2 cut(s) 17, 55
FspBI CTAG 7 cut(s) 384, 732, 753, 816, 1136, 1332, 1460
GlaI GCGC 1 cut(s) 20
GluI GCNGC 2 cut(s) 17, 55
GsuI CTGGAG 1 cut(s) 468
HaeII RGCGCY 1 cut(s) 22
HaeIII GGCC 4 cut(s) 123, 666, 1193, 1327
HapII CCGG 2 cut(s) 662, 1358
HhaI GCGC 1 cut(s) 21
Hin6I GCGC 1 cut(s) 19
HinP1I GCGC 1 cut(s) 19
HincII GTYRAC 1 cut(s) 646
HindII GTYRAC 1 cut(s) 646
HindIII AAGCTT 1 cut(s) 352
HpaI GTTAAC 1 cut(s) 646
HpaII CCGG 2 cut(s) 662, 1358
HphI GGTGA 3 cut(s) 206, 498, 1070
Hpy166II GTNNAC 2 cut(s) 646, 1375
Hpy188I TCNGA 7 cut(s) 39, 149, 412, 1263, 1453, 1663, 1678
Hpy8I GTNNAC 2 cut(s) 646, 1375
HpyAV CCTTC 9 cut(s) 417, 418, 638, 799, 955, 1017, 1448, 1532, 1673
HpyCH4III ACNGT 2 cut(s) 461, 1690
HpyF10VI GCNNNNNNNGC 4 cut(s) 16, 63, 305, 946
HpyF3I CTNAG 5 cut(s) 867, 1260, 1348, 1608, 1675
HspAI GCGC 1 cut(s) 19
Ksp22I TGATCA 2 cut(s) 571, 1050
KspAI GTTAAC 1 cut(s) 646
Kzo9I GATC 4 cut(s) 571, 580, 634, 1050
LmnI GCTCC 2 cut(s) 63, 589
Lsp1109I GCAGC 2 cut(s) 28, 41
LweI GCATC 6 cut(s) 60, 102, 308, 455, 1210, 1327
MaeI CTAG 7 cut(s) 384, 732, 753, 816, 1136, 1332, 1460
MaeIII GTNAC 6 cut(s) 838, 1143, 1240, 1284, 1508, 1707
MalI GATC 4 cut(s) 573, 582, 636, 1052
MboI GATC 4 cut(s) 571, 580, 634, 1050
MfeI CAATTG 2 cut(s) 11, 896
MflI RGATCY 1 cut(s) 634
MhlI GDGCHC 1 cut(s) 263
MluCI AATT 6 cut(s) 11, 477, 611, 623, 833, 896
MlyI GAGTC 5 cut(s) 509, 916, 1151, 1295, 1612
Mph1103I ATGCAT 3 cut(s) 75, 396, 942
MroXI GAANNNNTTC 1 cut(s) 807
MseI TTAA 8 cut(s) 621, 645, 695, 915, 1217, 1223, 1362, 1431
MslI CAYNNNNRTG 3 cut(s) 189, 373, 1530
MspA1I CMGCKG 1 cut(s) 538
MspI CCGG 2 cut(s) 662, 1358
MspR9I CCNGG 4 cut(s) 23, 120, 467, 663
MunI CAATTG 2 cut(s) 11, 896
Mva1269I GAATGC 1 cut(s) 1261
MvaI CCWGG 3 cut(s) 23, 120, 467
MwoI GCNNNNNNNGC 4 cut(s) 16, 63, 305, 946
NciI CCSGG 1 cut(s) 663
NcoI CCATGG 1 cut(s) 368
NdeII GATC 4 cut(s) 571, 580, 634, 1050
NlaIV GGNNCC 3 cut(s) 65, 429, 1414
NmuCI GTSAC 5 cut(s) 838, 1143, 1284, 1508, 1707
NsiI ATGCAT 3 cut(s) 75, 396, 942
NspI RCATGY 4 cut(s) 61, 944, 1701, 1731
NspV TTCGAA 1 cut(s) 846
PaeI GCATGC 1 cut(s) 944
PagI TCATGA 2 cut(s) 1525, 1573
PciI ACATGT 1 cut(s) 1727
PcsI WCGNNNNNNNCGW 1 cut(s) 35
PctI GAATGC 1 cut(s) 1261
PdmI GAANNNNTTC 1 cut(s) 807
PfeI GAWTC 6 cut(s) 144, 725, 848, 892, 1354, 1649
PkrI GCNGC 2 cut(s) 18, 56
PleI GAGTC 5 cut(s) 508, 915, 1150, 1295, 1611
PpsI GAGTC 5 cut(s) 508, 915, 1150, 1295, 1611
PpuMI RGGWCCY 2 cut(s) 859, 1519
PscI ACATGT 1 cut(s) 1727
Psp124BI GAGCTC 1 cut(s) 263
Psp5II RGGWCCY 2 cut(s) 859, 1519
Psp6I CCWGG 3 cut(s) 21, 118, 465
PspGI CCWGG 3 cut(s) 21, 118, 465
PspN4I GGNNCC 3 cut(s) 65, 429, 1414
PspPI GGNCC 7 cut(s) 664, 786, 859, 1325, 1375, 1412, 1519
PspPPI RGGWCCY 2 cut(s) 859, 1519
PsuI RGATCY 1 cut(s) 634
PvuII CAGCTG 1 cut(s) 538
RsaI GTAC 3 cut(s) 463, 820, 1642
RsaNI GTAC 3 cut(s) 462, 819, 1641
RseI CAYNNNNRTG 3 cut(s) 189, 373, 1530
SacI GAGCTC 1 cut(s) 263
SaqAI TTAA 8 cut(s) 621, 645, 695, 915, 1217, 1223, 1362, 1431
SatI GCNGC 2 cut(s) 17, 55
Sau3AI GATC 4 cut(s) 571, 580, 634, 1050
Sau96I GGNCC 7 cut(s) 664, 786, 859, 1325, 1375, 1412, 1519
ScaI AGTACT 1 cut(s) 820
SchI GAGTC 5 cut(s) 509, 916, 1151, 1295, 1612
ScrFI CCNGG 4 cut(s) 23, 120, 467, 663
SduI GDGCHC 1 cut(s) 263
SfaNI GCATC 6 cut(s) 60, 102, 308, 455, 1210, 1327
SfcI CTRYAG 1 cut(s) 717
SfuI TTCGAA 1 cut(s) 846
SinI GGWCC 5 cut(s) 786, 859, 1375, 1412, 1519
SmiMI CAYNNNNRTG 3 cut(s) 189, 373, 1530
SmlI CTYRAG 2 cut(s) 852, 1733
SmoI CTYRAG 2 cut(s) 852, 1733
SpeI ACTAGT 1 cut(s) 383
SphI GCATGC 1 cut(s) 944
Sse9I AATT 6 cut(s) 11, 477, 611, 623, 833, 896
SsiI CCGC 1 cut(s) 947
SspMI CTAG 7 cut(s) 384, 732, 753, 816, 1136, 1332, 1460
SstI GAGCTC 1 cut(s) 263
StyD4I CCNGG 4 cut(s) 21, 118, 465, 661
StyI CCWWGG 1 cut(s) 368
TaaI ACNGT 2 cut(s) 461, 1690
TaqI TCGA 2 cut(s) 163, 846
TaqII GACCGA 1 cut(s) 813
TasI AATT 6 cut(s) 11, 477, 611, 623, 833, 896
TatI WGTACW 2 cut(s) 818, 1640
TfiI GAWTC 6 cut(s) 144, 725, 848, 892, 1354, 1649
Tru1I TTAA 8 cut(s) 621, 645, 695, 915, 1217, 1223, 1362, 1431
Tru9I TTAA 8 cut(s) 621, 645, 695, 915, 1217, 1223, 1362, 1431
TscAI CASTG 3 cut(s) 988, 1060, 1716
TseFI GTSAC 5 cut(s) 838, 1143, 1284, 1508, 1707
TseI GCWGC 2 cut(s) 16, 54
Tsp45I GTSAC 5 cut(s) 838, 1143, 1284, 1508, 1707
TspDTI ATGAA 7 cut(s) 468, 699, 792, 1041, 1050, 1514, 1682
TspRI CASTG 3 cut(s) 988, 1060, 1716
VpaK11BI GGWCC 5 cut(s) 786, 859, 1375, 1412, 1519
XagI CCTNNNNNAGG 2 cut(s) 470, 866
XapI RAATTY 2 cut(s) 611, 623
XbaI TCTAGA 2 cut(s) 731, 1135
XceI RCATGY 4 cut(s) 61, 944, 1701, 1731
XmnI GAANNNNTTC 1 cut(s) 807
XspI CTAG 7 cut(s) 384, 732, 753, 816, 1136, 1332, 1460
ZrmI AGTACT 1 cut(s) 820
Zsp2I ATGCAT 3 cut(s) 75, 396, 942
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.