Rorug05G0364600

Domain of unknown function (DUF4378)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0008856
Physical Location & Seq
Forward (+)
48518841 .. 48521175
2335 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0364600.1

Sequence Viewer

Length: 882 bp
ATGGGGGGCTTTCTGGTCAGTATTGTGATGACTCCCTTTGTACAACTTTGGGGGAGACCATGGAATTTGCTAGACATAATCACAGCGGCTACTTTTCTAGGTCTCCATAGCCTCTGTTTGTTTGCTCCGTTTCATTTCACATGGGCCGCCTTTTGGGTGGCCGCCGCACTCTACATTCTCACCGGTTTGGGAGTCACTCTGGGTTTGCATAGACACCTTGCGCACAAGAGTTTACGGCTTCCCAAATGGCTCGAGTACTTGTTTGCTTATTTTGGGGTTCTAGCACTTCAGGGAAGTCCGATAGAATGGGTGAGCACACACCGATACCATCACCAGTTTACAGACACAGAGAGAGACCCCCACAGCCCCTTGAAGGGTTTCTGGTTTAGTCATATGGGCTGGATCCTTGATAGCACTTCTCGGTTTCAAAAATATGGAGGACTGAAGAACGTCGAGGATATGAAAAAGCAGTGGTTCTACAGATTTCTTCATCGTACATTCCTTGTACACATATTTGTTTTTGGAGCTTTGCTCTACGCCCTAGGTGGACTACCCTTCTTTATCTGGGGAATGGGAGTGAGGATGGTATATGTTTTCCACACCACTTTGCTAGTAAACTCGGTCGGTCACATCTGGGGAAAACAAGTATGGAACACCGGCGACCTGTCAAAAAACAATTGGTTGTTGGGATGGATCGCACTTGGAGAAGGGTGGCACAACAACCATCATGCATTTGAATACTCAGCTCGACAAGGTCTGGAATGGTGGCAAGTCGACTTGACTTGGTATGTAATCAGAGCTCTTCAAGAAATTGGGTTGGCAACTGACGTCAAAGTTCCCACTGAATCTCAGATGGGTCGCAAAGCCCTCAAGAAATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3741 DUF4378

Protein Analysis

293

Amino Acids

34.06

Weight (kDa)

9.54

Isoelectric Point (pI)

34.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase PF00487 47 - 267 1.5e-20 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 831
Acc16I TGCGCA 1 cut(s) 222
AccI GTMKAC 1 cut(s) 774
AciI CCGC 4 cut(s) 86, 147, 162, 165
AclWI GGATC 3 cut(s) 397, 410, 701
AcoI YGGCCR 1 cut(s) 159
AcsI RAATTY 1 cut(s) 64
AcuI CTGAAG 2 cut(s) 272, 464
AcyI GRCGYC 1 cut(s) 828
AfaI GTAC 4 cut(s) 42, 257, 496, 507
AfiI CCNNNNNNNGG 3 cut(s) 153, 373, 374
AgeI ACCGGT 1 cut(s) 182
AgsI TTSAA 4 cut(s) 373, 428, 737, 806
AluBI AGCT 3 cut(s) 527, 746, 800
AluI AGCT 3 cut(s) 527, 746, 800
Alw21I GWGCWC 2 cut(s) 317, 802
Alw26I GTCTC 3 cut(s) 49, 107, 348
AlwI GGATC 3 cut(s) 397, 410, 701
Ama87I CYCGRG 1 cut(s) 251
AoxI GGCC 2 cut(s) 144, 159
ApoI RAATTY 1 cut(s) 64
AsiGI ACCGGT 1 cut(s) 182
Asp700I GAANNNNTTC 1 cut(s) 377
AspA2I CCTAGG 1 cut(s) 541
AspLEI GCGC 1 cut(s) 223
AspS9I GGNCC 1 cut(s) 144
AsuHPI GGTGA 3 cut(s) 172, 322, 323
AvaI CYCGRG 1 cut(s) 251
AvrII CCTAGG 1 cut(s) 541
BamHI GGATCC 1 cut(s) 402
BanII GRGCYC 1 cut(s) 802
Bbv12I GWGCWC 2 cut(s) 317, 802
BccI CCATC 5 cut(s) 336, 577, 684, 732, 847
BceAI ACGGC 1 cut(s) 251
BcoDI GTCTC 3 cut(s) 49, 107, 348
BfaI CTAG 5 cut(s) 71, 98, 281, 542, 611
BfmI CTRYAG 1 cut(s) 478
BisI GCNGC 4 cut(s) 87, 147, 162, 165
BlnI CCTAGG 1 cut(s) 541
BlsI GCNGC 4 cut(s) 88, 148, 163, 166
BmcAI AGTACT 1 cut(s) 257
BmeT110I CYCGRG 1 cut(s) 251
BmgT120I GGNCC 1 cut(s) 144
BmiI GGNNCC 1 cut(s) 404
BplI GAGNNNNNCTC 2 cut(s) 516, 548
BpuEI CTTGAG 1 cut(s) 854
BsaHI GRCGYC 1 cut(s) 828
BsaI GGTCTC 3 cut(s) 49, 107, 348
BsaJI CCNNGG 2 cut(s) 59, 541
BsaWI WCCGGW 1 cut(s) 182
Bsc4I CCNNNNNNNGG 3 cut(s) 153, 373, 374
Bse118I RCCGGY 2 cut(s) 182, 656
Bse1I ACTGG 1 cut(s) 334
BseDI CCNNGG 2 cut(s) 59, 541
BseGI GGATG 2 cut(s) 588, 695
BseLI CCNNNNNNNGG 3 cut(s) 153, 373, 374
BseMII CTCAG 2 cut(s) 756, 863
BseNI ACTGG 1 cut(s) 334
Bsh1285I CGRYCG 1 cut(s) 624
BshFI GGCC 2 cut(s) 146, 161
BshTI ACCGGT 1 cut(s) 182
BsiEI CGRYCG 1 cut(s) 624
BsiHKAI GWGCWC 2 cut(s) 317, 802
BsiHKCI CYCGRG 1 cut(s) 251
BsiSI CCGG 2 cut(s) 183, 657
BslI CCNNNNNNNGG 3 cut(s) 153, 373, 374
BsmAI GTCTC 3 cut(s) 49, 107, 348
BsnI GGCC 2 cut(s) 146, 161
Bso31I GGTCTC 3 cut(s) 49, 107, 348
BsoBI CYCGRG 1 cut(s) 251
Bsp1286I GDGCHC 2 cut(s) 317, 802
Bsp1407I TGTACA 2 cut(s) 40, 505
Bsp143I GATC 2 cut(s) 402, 693
Bsp19I CCATGG 1 cut(s) 59
BspACI CCGC 4 cut(s) 86, 147, 162, 165
BspANI GGCC 2 cut(s) 146, 161
BspCNI CTCAG 2 cut(s) 755, 862
BspLI GGNNCC 1 cut(s) 404
BspPI GGATC 3 cut(s) 397, 410, 701
BspQI GCTCTTC 1 cut(s) 807
BspTNI GGTCTC 3 cut(s) 49, 107, 348
BsrFI RCCGGY 2 cut(s) 182, 656
BsrGI TGTACA 2 cut(s) 40, 505
BsrI ACTGG 1 cut(s) 334
BssAI RCCGGY 2 cut(s) 182, 656
BssECI CCNNGG 2 cut(s) 59, 541
BssMI GATC 2 cut(s) 402, 693
BssNI GRCGYC 1 cut(s) 828
BssT1I CCWWGG 2 cut(s) 59, 541
Bst6I CTCTTC 1 cut(s) 807
BstACI GRCGYC 1 cut(s) 828
BstAUI TGTACA 2 cut(s) 40, 505
BstDEI CTNAG 2 cut(s) 742, 849
BstDSI CCRYGG 1 cut(s) 59
BstF5I GGATG 2 cut(s) 588, 695
BstHHI GCGC 1 cut(s) 223
BstKTI GATC 2 cut(s) 405, 696
BstMAI GTCTC 3 cut(s) 49, 107, 348
BstMBI GATC 2 cut(s) 402, 693
BstMCI CGRYCG 1 cut(s) 624
BstSFI CTRYAG 1 cut(s) 478
BstX2I RGATCY 1 cut(s) 402
BstYI RGATCY 1 cut(s) 402
BsuRI GGCC 2 cut(s) 146, 161
BtgI CCRYGG 1 cut(s) 59
BtsCI GGATG 2 cut(s) 588, 695
BtsI GCAGTG 1 cut(s) 476
BtsIMutI CAGTG 2 cut(s) 476, 840
CfoI GCGC 1 cut(s) 223
Cfr10I RCCGGY 2 cut(s) 182, 656
Cfr13I GGNCC 1 cut(s) 144
Csp6I GTAC 4 cut(s) 41, 256, 495, 506
CspAI ACCGGT 1 cut(s) 182
CviAII CATG 3 cut(s) 60, 141, 728
CviQI GTAC 4 cut(s) 41, 256, 495, 506
DdeI CTNAG 2 cut(s) 742, 849
DpnI GATC 2 cut(s) 404, 695
DpnII GATC 2 cut(s) 402, 693
EaeI YGGCCR 1 cut(s) 159
Eam1104I CTCTTC 1 cut(s) 807
EarI CTCTTC 1 cut(s) 807
Ecl136II GAGCTC 1 cut(s) 800
Eco130I CCWWGG 2 cut(s) 59, 541
Eco24I GRGCYC 1 cut(s) 802
Eco31I GGTCTC 3 cut(s) 49, 107, 348
Eco53kI GAGCTC 1 cut(s) 800
Eco57I CTGAAG 2 cut(s) 272, 464
Eco88I CYCGRG 1 cut(s) 251
EcoICRI GAGCTC 1 cut(s) 800
EcoT14I CCWWGG 2 cut(s) 59, 541
EcoT22I ATGCAT 1 cut(s) 733
EcoT38I GRGCYC 1 cut(s) 802
ErhI CCWWGG 2 cut(s) 59, 541
FaeI CATG 3 cut(s) 63, 144, 731
FatI CATG 3 cut(s) 59, 140, 727
FauNDI CATATG 1 cut(s) 393
FblI GTMKAC 1 cut(s) 774
Fnu4HI GCNGC 4 cut(s) 87, 147, 162, 165
FokI GGATG 2 cut(s) 595, 702
FriOI GRGCYC 1 cut(s) 802
Fsp4HI GCNGC 4 cut(s) 87, 147, 162, 165
FspBI CTAG 5 cut(s) 71, 98, 281, 542, 611
FspI TGCGCA 1 cut(s) 222
GlaI GCGC 1 cut(s) 222
GluI GCNGC 4 cut(s) 87, 147, 162, 165
HaeIII GGCC 2 cut(s) 146, 161
HapII CCGG 2 cut(s) 183, 657
HhaI GCGC 1 cut(s) 223
Hin1I GRCGYC 1 cut(s) 828
Hin1II CATG 3 cut(s) 63, 144, 731
Hin6I GCGC 1 cut(s) 221
HinP1I GCGC 1 cut(s) 221
HincII GTYRAC 1 cut(s) 775
HindII GTYRAC 1 cut(s) 775
HinfI GANTC 3 cut(s) 31, 192, 845
HpaII CCGG 2 cut(s) 183, 657
HphI GGTGA 3 cut(s) 172, 322, 323
Hpy166II GTNNAC 6 cut(s) 233, 339, 508, 548, 616, 775
Hpy188I TCNGA 3 cut(s) 300, 797, 852
Hpy188III TCNNGA 3 cut(s) 758, 806, 871
Hpy8I GTNNAC 6 cut(s) 233, 339, 508, 548, 616, 775
Hpy99I CGWCG 1 cut(s) 455
HpyAV CCTTC 3 cut(s) 367, 565, 701
HpyCH4IV ACGT 2 cut(s) 450, 828
HpyCH4V TGCA 2 cut(s) 208, 731
HpyF3I CTNAG 2 cut(s) 742, 849
HpySE526I ACGT 2 cut(s) 450, 828
Hsp92I GRCGYC 1 cut(s) 828
Hsp92II CATG 3 cut(s) 63, 144, 731
HspAI GCGC 1 cut(s) 221
Kzo9I GATC 2 cut(s) 402, 693
LguI GCTCTTC 1 cut(s) 807
LmnI GCTCC 2 cut(s) 130, 524
MaeI CTAG 5 cut(s) 71, 98, 281, 542, 611
MaeII ACGT 2 cut(s) 450, 828
MaeIII GTNAC 2 cut(s) 193, 626
MalI GATC 2 cut(s) 404, 695
MboI GATC 2 cut(s) 402, 693
MboII GAAGA 3 cut(s) 457, 479, 794
MfeI CAATTG 1 cut(s) 676
MflI RGATCY 1 cut(s) 402
MhlI GDGCHC 2 cut(s) 317, 802
MluCI AATT 3 cut(s) 64, 676, 810
MlyI GAGTC 2 cut(s) 25, 201
MnlI CCTC 5 cut(s) 122, 431, 448, 573, 878
Mph1103I ATGCAT 1 cut(s) 733
MroXI GAANNNNTTC 1 cut(s) 377
MspA1I CMGCKG 1 cut(s) 86
MspI CCGG 2 cut(s) 183, 657
MunI CAATTG 1 cut(s) 676
NcoI CCATGG 1 cut(s) 59
NdeI CATATG 1 cut(s) 393
NdeII GATC 2 cut(s) 402, 693
NlaIII CATG 3 cut(s) 63, 144, 731
NlaIV GGNNCC 1 cut(s) 404
NmuCI GTSAC 2 cut(s) 193, 626
NsbI TGCGCA 1 cut(s) 222
NsiI ATGCAT 1 cut(s) 733
PaeR7I CTCGAG 1 cut(s) 251
PciSI GCTCTTC 1 cut(s) 807
PdmI GAANNNNTTC 1 cut(s) 377
PfeI GAWTC 1 cut(s) 845
PflFI GACNNNGTC 1 cut(s) 753
PinAI ACCGGT 1 cut(s) 182
PkrI GCNGC 4 cut(s) 88, 148, 163, 166
PleI GAGTC 2 cut(s) 25, 200
PpsI GAGTC 2 cut(s) 25, 200
Psp124BI GAGCTC 1 cut(s) 802
PspN4I GGNNCC 1 cut(s) 404
PspPI GGNCC 1 cut(s) 144
PspXI VCTCGAGB 1 cut(s) 251
PsuI RGATCY 1 cut(s) 402
PsyI GACNNNGTC 1 cut(s) 753
RsaI GTAC 4 cut(s) 42, 257, 496, 507
RsaNI GTAC 4 cut(s) 41, 256, 495, 506
SacI GAGCTC 1 cut(s) 802
SalI GTCGAC 1 cut(s) 773
SapI GCTCTTC 1 cut(s) 807
SatI GCNGC 4 cut(s) 87, 147, 162, 165
Sau3AI GATC 2 cut(s) 402, 693
Sau96I GGNCC 1 cut(s) 144
ScaI AGTACT 1 cut(s) 257
SchI GAGTC 2 cut(s) 25, 201
SduI GDGCHC 2 cut(s) 317, 802
SfcI CTRYAG 1 cut(s) 478
Sfr274I CTCGAG 1 cut(s) 251
SgrAI CRCCGGYG 1 cut(s) 656
SlaI CTCGAG 1 cut(s) 251
SmlI CTYRAG 2 cut(s) 251, 869
SmoI CTYRAG 2 cut(s) 251, 869
Sse9I AATT 3 cut(s) 64, 676, 810
SsiI CCGC 4 cut(s) 86, 147, 162, 165
SspI AATATT 1 cut(s) 878
SspMI CTAG 5 cut(s) 71, 98, 281, 542, 611
SstI GAGCTC 1 cut(s) 802
StyI CCWWGG 2 cut(s) 59, 541
TaiI ACGT 2 cut(s) 453, 831
TaqI TCGA 4 cut(s) 252, 453, 748, 774
TaqII GACCGA 2 cut(s) 610, 614
TasI AATT 3 cut(s) 64, 676, 810
TatI WGTACW 3 cut(s) 40, 255, 505
TauI GCSGC 4 cut(s) 89, 149, 164, 167
TfiI GAWTC 1 cut(s) 845
TscAI CASTG 2 cut(s) 476, 847
TseFI GTSAC 2 cut(s) 193, 626
Tsp45I GTSAC 2 cut(s) 193, 626
TspDTI ATGAA 3 cut(s) 122, 476, 479
TspGWI ACGGA 1 cut(s) 117
TspRI CASTG 2 cut(s) 476, 847
Tth111I GACNNNGTC 1 cut(s) 753
XapI RAATTY 1 cut(s) 64
XhoI CTCGAG 1 cut(s) 251
XmaJI CCTAGG 1 cut(s) 541
XmiI GTMKAC 1 cut(s) 774
XmnI GAANNNNTTC 1 cut(s) 377
XspI CTAG 5 cut(s) 71, 98, 281, 542, 611
ZraI GACGTC 1 cut(s) 829
ZrmI AGTACT 1 cut(s) 257
Zsp2I ATGCAT 1 cut(s) 733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.