Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0000752

Physical Location & Seq

Genomic Coordinates Reverse (-)

12464807 .. 12470082

Gene Structure 5276 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1135900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1518 bp

ATGTTGGGTTTATTGACAATGCGCTTGTTCTGGTGGCAACAGCTGCCTCTGCTTATTCTCATCCATCTTTTGGAACCCAGCAGAACAAGCTACAAAGTTTACGCTGAAGCTCTACTAACTTTCAAGAGCGATGGTGGAAGCCCTCAACTACTCAACGGTCGAATTGGAAGATGTCCTTGTCTAACACAATGGAAAATTGTCTACTGTCATAAACTGAAGGACCCGTGTGTTGTTTCTTTACTTTTCGTTACACACTCTTCAATTCAACTACAAAAAGAGCACCATAAACCAAGAAGCAGGAGGATAGCAGCCATAGTTGATGGGGATAATGGAACTCCAACACTCACACCTGTGGAAAAAGTTCGTGGTAACCATTCAACAAAACCAAAAGTTGCAGCAATTATTGGTGGAGTTGTTGCCACTCTGCTTGTGATTATCGTAGTGATGCTCGTTTACATCTGCTTGATGCGTGTAAAAAGATTAACTAGGCAAACATCTGAGACAGTGTCATCTGCACCATCACCCCCTGTTCAGTGGGAAAGAGGGGACGCATCTCCCTACGCCATTGCTCATTCTCCTTACAGTGCACCAAACTTGAGGCAACTCACCATATTGGAATTGGAGCAAGCGACGTGCAATTTCAGTGAAACTAATATCATAAGCCAAGGTAGATTTGGTTTGGTTTACAAGGGATTGCTTCAAGATGGGACTATTGTAGCTATCAAGAGACGCCTACATGCCCCAACTCAGTATTTCTTTCATGAGGTGAAGCACGTAGCTCGTGTCAACCACATGCATATTCTCAGGCTTATTGGTTATTGTCAAGATGCCACTCAACAGTTACTTGTATATGACTATCTTCCAAATGGAAATGTTGGGAATCATCTATACGATGCTGAAGGTTTGCCTATTGGAAGGTTGGGCATAAGGCAAAGGCTATCGATTGCTTTGGGTGCAGCCAAAGGACTTGCACACCTTCGCAGTTTGGTGCCTCCTCTTCTGCACATGCATTTTAGAACAAGCAACGTTCTTCTGGACGAAAACTTTACTGCCAAGGTGTCTGATTATGGACTAACCAAATTGCTAGTGGGTCATCATGCTGGCTCATCTTCAGCCGTTGATTGCTTTGTTGACCCAGAGTTAGATTCATCAAAGAAGTTTTCAGAGAGAAGTGATGTTTACAGTTTCGGGGTCTTCTTGCTGGAATTGGTCAGTGGACGTGAAGCGAATGCAAGAAACCAGTTGAACTCAGTGGATAATTTAATATTGCAGGCAAAGGATTGCAAGGATTTAGACAGATTTGTTGACAAAACTTTAGGTGACAAGTCGAGGCAAACTGCGAAACAGATGATGGAGCTGGCATTGATGTGTGTAGATGGTAGCGAACGAAGGCCGCAGATGCAGATTGTTGTGGAGGAGCTAGAACGAATCCATCGGGGGAGAGAAAGTAGCCATTTTCACATAGAAGTTGATGAGGAGATAGGTGCTGTGACTCTTGGGAGTGAGCTTTTCAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase_Tyr

Protein Analysis

506

Amino Acids

56.55

Weight (kDa)

8.96

Isoelectric Point (pI)

41.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	216 - 465	1.1e-24	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	218 - 474	4.7e-32	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000752)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g32300 FvH4_3g32300 FvH4_3g32300 FvH4_3g32300 FvH4_3g32300 FvH4_3g32300 FvH4_3g32300 FvH4_3g32300 FvH4_3g32300 FvH4_3g32300 FvH4_3g32300 FvH4_3g32300 FvH4_3g32300 FvH4_3g32300 FvH4_3g32300
malus_domestica	MD03G1117600.v1.1 MD11G1135900.v1.1
prunus_persica	Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1 Prupe.6G101300_v2.0.a1
pyrus_communis	pycom11g11270
rosa_chinensis	RchiOBHm_Chr5g0059091
rosa_laevigata	RLG00000035279
rosa_multiflora	Rmu_sc0002983.1_g000039
rosa_roxburghii	Rroxscaffold_1G00021320
rosa_rugosa	Rorug05G0320400 Rorug05G0320500 Rorug05G0320600 Rorug05G0320700 Rorug05G0320800 Rorug05G0320900 Rorug05G0321000 Rorug05G0321000 Rorug05G0321100
rosa_samantha	Rh5AG385800 Rh5BG397300 Rh5CG421000 Rh5DG413400
rosa_wichuraiana	Rw5G036320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	988
AccB7I	CCANNNNNTGG	1 cut(s)	70
AccI	GTMKAC	1 cut(s)	201
AciI	CCGC	1 cut(s)	1394
AclI	AACGTT	1 cut(s)	1026
AcuI	CTGAAG	4 cut(s)	126, 236, 918, 1095
AcyI	GRCGYC	1 cut(s)	730
AfiI	CCNNNNNNNGG	1 cut(s)	70
AgsI	TTSAA	7 cut(s)	124, 261, 266, 378, 701, 1246, 1513
AjiI	CACGTC	2 cut(s)	633, 1220
AleI	CACNNNNGTG	1 cut(s)	350
AluBI	AGCT	8 cut(s)	43, 90, 110, 719, 779, 1357, 1420, 1507
AluI	AGCT	8 cut(s)	43, 90, 110, 719, 779, 1357, 1420, 1507
Alw21I	GWGCWC	2 cut(s)	282, 589
Alw26I	GTCTC	2 cut(s)	494, 721
Alw44I	GTGCAC	1 cut(s)	585
AoxI	GGCC	1 cut(s)	1391
ApaLI	GTGCAC	1 cut(s)	585
ApeKI	GCWGC	4 cut(s)	43, 308, 395, 956
Asp700I	GAANNNNTTC	1 cut(s)	360
AspLEI	GCGC	1 cut(s)	24
AspS9I	GGNCC	1 cut(s)	220
AsuHPI	GGTGA	4 cut(s)	513, 598, 778, 1331
AvaII	GGWCC	1 cut(s)	220
BaeGI	GKGCMC	1 cut(s)	589
BanI	GGYRCC	1 cut(s)	988
BauI	CACGAG	1 cut(s)	780
BbsI	GAAGAC	1 cut(s)	1186
Bbv12I	GWGCWC	2 cut(s)	282, 589
BbvI	GCAGC	4 cut(s)	30, 320, 407, 968
BccI	CCATC	8 cut(s)	72, 125, 314, 526, 698, 1345, 1370, 1440
BceAI	ACGGC	1 cut(s)	1100
BcgI	CGANNNNNNTGC	2 cut(s)	761, 795
BcoDI	GTCTC	2 cut(s)	494, 721
BfaI	CTAG	3 cut(s)	486, 1085, 1421
BisI	GCNGC	5 cut(s)	44, 309, 396, 957, 1394
BlsI	GCNGC	5 cut(s)	45, 310, 397, 958, 1395
Bme18I	GGWCC	1 cut(s)	220
BmgBI	CACGTC	2 cut(s)	633, 1220
BmgT120I	GGNCC	1 cut(s)	220
BmiI	GGNNCC	3 cut(s)	75, 222, 990
BmsI	GCATC	6 cut(s)	435, 456, 560, 817, 883, 1389
BpiI	GAAGAC	1 cut(s)	1186
BpuEI	CTTGAG	1 cut(s)	616
Bsa29I	ATCGAT	1 cut(s)	941
BsaAI	YACGTR	1 cut(s)	775
BsaHI	GRCGYC	1 cut(s)	730
BsaJI	CCNNGG	2 cut(s)	664, 1053
BsaXI	ACNNNNNCTCC	2 cut(s)	1432, 1462
Bsc4I	CCNNNNNNNGG	1 cut(s)	70
Bse1I	ACTGG	1 cut(s)	1240
Bse3DI	GCAATG	1 cut(s)	564
BseCI	ATCGAT	1 cut(s)	941
BseDI	CCNNGG	2 cut(s)	664, 1053
BseGI	GGATG	1 cut(s)	60
BseLI	CCNNNNNNNGG	1 cut(s)	70
BseMI	GCAATG	1 cut(s)	564
BseMII	CTCAG	4 cut(s)	489, 761, 817, 1263
BseNI	ACTGG	1 cut(s)	1240
BseRI	GAGGAG	3 cut(s)	984, 1430, 1490
BseSI	GKGCMC	1 cut(s)	589
BseXI	GCAGC	4 cut(s)	30, 320, 407, 968
BseYI	CCCAGC	1 cut(s)	77
BsgI	GTGCAG	3 cut(s)	498, 975, 986
Bsh1285I	CGRYCG	1 cut(s)	160
BshFI	GGCC	1 cut(s)	1393
BshNI	GGYRCC	1 cut(s)	988
BshVI	ATCGAT	1 cut(s)	941
BsiEI	CGRYCG	1 cut(s)	160
BsiHKAI	GWGCWC	2 cut(s)	282, 589
BslFI	GGGAC	2 cut(s)	560, 721
BslI	CCNNNNNNNGG	1 cut(s)	70
BsmAI	GTCTC	2 cut(s)	494, 721
BsmBI	CGTCTC	1 cut(s)	721
BsmFI	GGGAC	2 cut(s)	560, 721
BsmI	GAATGC	1 cut(s)	1234
BsnI	GGCC	1 cut(s)	1393
Bsp1286I	GDGCHC	2 cut(s)	282, 589
BspACI	CCGC	1 cut(s)	1394
BspANI	GGCC	1 cut(s)	1393
BspCNI	CTCAG	4 cut(s)	490, 760, 816, 1262
BspDI	ATCGAT	1 cut(s)	941
BspHI	TCATGA	1 cut(s)	760
BspLI	GGNNCC	3 cut(s)	75, 222, 990
BspT107I	GGYRCC	1 cut(s)	988
BsrDI	GCAATG	1 cut(s)	564
BsrI	ACTGG	1 cut(s)	1240
BssECI	CCNNGG	2 cut(s)	664, 1053
BssNI	GRCGYC	1 cut(s)	730
BssSI	CACGAG	1 cut(s)	780
BssT1I	CCWWGG	2 cut(s)	664, 1053
Bst2BI	CACGAG	1 cut(s)	780
Bst4CI	ACNGT	6 cut(s)	158, 206, 505, 584, 840, 1184
Bst6I	CTCTTC	2 cut(s)	262, 1002
BstACI	GRCGYC	1 cut(s)	730
BstAPI	GCANNNNNTGC	1 cut(s)	43
BstBAI	YACGTR	1 cut(s)	775
BstC8I	GCNNGC	4 cut(s)	627, 1102, 1272, 1359
BstDEI	CTNAG	4 cut(s)	498, 747, 803, 1249
BstEII	GGTNACC	1 cut(s)	368
BstF5I	GGATG	1 cut(s)	60
BstHHI	GCGC	1 cut(s)	24
BstMAI	GTCTC	2 cut(s)	494, 721
BstMCI	CGRYCG	1 cut(s)	160
BstMWI	GCNNNNNNNGC	5 cut(s)	43, 49, 87, 953, 1399
BstNSI	RCATGY	3 cut(s)	740, 796, 1009
BstPI	GGTNACC	1 cut(s)	368
BstSLI	GKGCMC	1 cut(s)	589
BstV1I	GCAGC	4 cut(s)	30, 320, 407, 968
BstV2I	GAAGAC	1 cut(s)	1186
Bsu15I	ATCGAT	1 cut(s)	941
BsuRI	GGCC	1 cut(s)	1393
BsuTUI	ATCGAT	1 cut(s)	941
BtgZI	GCGATG	1 cut(s)	144
BtrI	CACGTC	2 cut(s)	633, 1220
BtsCI	GGATG	1 cut(s)	60
BtsIMutI	CAGTG	6 cut(s)	510, 539, 589, 649, 1219, 1257
Cac8I	GCNNGC	4 cut(s)	627, 1102, 1272, 1359
CciI	TCATGA	1 cut(s)	760
CfoI	GCGC	1 cut(s)	24
Cfr13I	GGNCC	1 cut(s)	220
ClaI	ATCGAT	1 cut(s)	941
CseI	GACGC	2 cut(s)	557, 738
CspCI	CAANNNNNGTGG	2 cut(s)	409, 444
CviAII	CATG	5 cut(s)	737, 761, 793, 1006, 1097
DdeI	CTNAG	4 cut(s)	498, 747, 803, 1249
Eam1104I	CTCTTC	2 cut(s)	262, 1002
EarI	CTCTTC	2 cut(s)	262, 1002
Eco130I	CCWWGG	2 cut(s)	664, 1053
Eco47I	GGWCC	1 cut(s)	220
Eco57I	CTGAAG	4 cut(s)	126, 236, 918, 1095
Eco91I	GGTNACC	1 cut(s)	368
EcoO109I	RGGNCCY	1 cut(s)	220
EcoO65I	GGTNACC	1 cut(s)	368
EcoT14I	CCWWGG	2 cut(s)	664, 1053
EcoT22I	ATGCAT	2 cut(s)	798, 1011
ErhI	CCWWGG	2 cut(s)	664, 1053
Esp3I	CGTCTC	1 cut(s)	721
FaeI	CATG	5 cut(s)	740, 764, 796, 1009, 1100
FalI	AAGNNNNNCTT	2 cut(s)	160, 192
FaqI	GGGAC	2 cut(s)	560, 721
FatI	CATG	5 cut(s)	736, 760, 792, 1005, 1096
FblI	GTMKAC	1 cut(s)	201
Fnu4HI	GCNGC	5 cut(s)	44, 309, 396, 957, 1394
FokI	GGATG	1 cut(s)	47
Fsp4HI	GCNGC	5 cut(s)	44, 309, 396, 957, 1394
FspBI	CTAG	3 cut(s)	486, 1085, 1421
GlaI	GCGC	1 cut(s)	23
GluI	GCNGC	5 cut(s)	44, 309, 396, 957, 1394
GsaI	CCCAGC	1 cut(s)	81
HaeIII	GGCC	1 cut(s)	1393
HgaI	GACGC	2 cut(s)	557, 738
HhaI	GCGC	1 cut(s)	24
Hin1I	GRCGYC	1 cut(s)	730
Hin1II	CATG	5 cut(s)	740, 764, 796, 1009, 1100
Hin6I	GCGC	1 cut(s)	22
HinP1I	GCGC	1 cut(s)	22
HincII	GTYRAC	3 cut(s)	787, 1132, 1306
HindII	GTYRAC	3 cut(s)	787, 1132, 1306
HinfI	GANTC	4 cut(s)	880, 1145, 1428, 1492
HphI	GGTGA	4 cut(s)	513, 598, 778, 1331
Hpy188I	TCNGA	3 cut(s)	499, 1063, 1165
Hpy188III	TCNNGA	6 cut(s)	124, 701, 724, 761, 824, 1034
Hpy99I	CGWCG	1 cut(s)	634
HpyAV	CCTTC	5 cut(s)	211, 893, 909, 986, 1383
HpyCH4III	ACNGT	6 cut(s)	158, 206, 505, 584, 840, 1184
HpyCH4IV	ACGT	4 cut(s)	632, 774, 1026, 1219
HpyF10VI	GCNNNNNNNGC	5 cut(s)	43, 49, 87, 953, 1399
HpyF3I	CTNAG	4 cut(s)	498, 747, 803, 1249
HpySE526I	ACGT	4 cut(s)	632, 774, 1026, 1219
Hsp92I	GRCGYC	1 cut(s)	730
Hsp92II	CATG	5 cut(s)	740, 764, 796, 1009, 1100
HspAI	GCGC	1 cut(s)	22
LmnI	GCTCC	3 cut(s)	622, 1354, 1417
Lsp1109I	GCAGC	4 cut(s)	30, 320, 407, 968
LweI	GCATC	6 cut(s)	435, 456, 560, 817, 883, 1389
MaeI	CTAG	3 cut(s)	486, 1085, 1421
MaeII	ACGT	4 cut(s)	632, 774, 1026, 1219
MaeIII	GTNAC	5 cut(s)	247, 368, 840, 1319, 1489
MboII	GAAGA	7 cut(s)	180, 249, 851, 989, 1022, 1101, 1186
MhlI	GDGCHC	2 cut(s)	282, 589
MluCI	AATT	9 cut(s)	162, 195, 261, 399, 617, 637, 1079, 1205, 1258
MlyI	GAGTC	1 cut(s)	1486
MmeI	TCCRAC	1 cut(s)	362
Mph1103I	ATGCAT	2 cut(s)	798, 1011
MroXI	GAANNNNTTC	1 cut(s)	360
MseI	TTAA	2 cut(s)	482, 1262
MslI	CAYNNNNRTG	2 cut(s)	350, 1366
MspA1I	CMGCKG	1 cut(s)	43
Mva1269I	GAATGC	1 cut(s)	1234
MwoI	GCNNNNNNNGC	5 cut(s)	43, 49, 87, 953, 1399
NlaIII	CATG	5 cut(s)	740, 764, 796, 1009, 1100
NlaIV	GGNNCC	3 cut(s)	75, 222, 990
NmuCI	GTSAC	2 cut(s)	1319, 1489
NsiI	ATGCAT	2 cut(s)	798, 1011
NspI	RCATGY	3 cut(s)	740, 796, 1009
OliI	CACNNNNGTG	1 cut(s)	350
PagI	TCATGA	1 cut(s)	760
PctI	GAATGC	1 cut(s)	1234
PdmI	GAANNNNTTC	1 cut(s)	360
PfeI	GAWTC	3 cut(s)	880, 1145, 1428
PflFI	GACNNNGTC	1 cut(s)	505
PflMI	CCANNNNNTGG	1 cut(s)	70
PkrI	GCNGC	5 cut(s)	45, 310, 397, 958, 1395
PleI	GAGTC	1 cut(s)	1486
PpsI	GAGTC	1 cut(s)	1486
Ppu21I	YACGTR	1 cut(s)	775
PpuMI	RGGWCCY	1 cut(s)	220
Psp1406I	AACGTT	1 cut(s)	1026
Psp5II	RGGWCCY	1 cut(s)	220
PspEI	GGTNACC	1 cut(s)	368
PspFI	CCCAGC	1 cut(s)	77
PspN4I	GGNNCC	3 cut(s)	75, 222, 990
PspPI	GGNCC	1 cut(s)	220
PspPPI	RGGWCCY	1 cut(s)	220
PsyI	GACNNNGTC	1 cut(s)	505
PvuII	CAGCTG	1 cut(s)	43
RseI	CAYNNNNRTG	2 cut(s)	350, 1366
SaqAI	TTAA	2 cut(s)	482, 1262
SatI	GCNGC	5 cut(s)	44, 309, 396, 957, 1394
Sau96I	GGNCC	1 cut(s)	220
SchI	GAGTC	1 cut(s)	1486
SduI	GDGCHC	2 cut(s)	282, 589
SfaNI	GCATC	6 cut(s)	435, 456, 560, 817, 883, 1389
SinI	GGWCC	1 cut(s)	220
SmiMI	CAYNNNNRTG	2 cut(s)	350, 1366
SmlI	CTYRAG	1 cut(s)	595
SmoI	CTYRAG	1 cut(s)	595
Sse9I	AATT	9 cut(s)	162, 195, 261, 399, 617, 637, 1079, 1205, 1258
SsiI	CCGC	1 cut(s)	1394
SspI	AATATT	1 cut(s)	1266
SspMI	CTAG	3 cut(s)	486, 1085, 1421
StyI	CCWWGG	2 cut(s)	664, 1053
TaaI	ACNGT	6 cut(s)	158, 206, 505, 584, 840, 1184
TaiI	ACGT	4 cut(s)	635, 777, 1029, 1222
TaqI	TCGA	3 cut(s)	160, 941, 1328
TasI	AATT	9 cut(s)	162, 195, 261, 399, 617, 637, 1079, 1205, 1258
TauI	GCSGC	1 cut(s)	1396
TfiI	GAWTC	3 cut(s)	880, 1145, 1428
Tru1I	TTAA	2 cut(s)	482, 1262
Tru9I	TTAA	2 cut(s)	482, 1262
TscAI	CASTG	6 cut(s)	510, 539, 589, 649, 1219, 1257
TseFI	GTSAC	2 cut(s)	1319, 1489
TseI	GCWGC	4 cut(s)	43, 308, 395, 956
Tsp45I	GTSAC	2 cut(s)	1319, 1489
TspDTI	ATGAA	2 cut(s)	749, 1137
TspRI	CASTG	6 cut(s)	510, 539, 589, 649, 1219, 1257
Tth111I	GACNNNGTC	1 cut(s)	505
Van91I	CCANNNNNTGG	1 cut(s)	70
VneI	GTGCAC	1 cut(s)	585
VpaK11BI	GGWCC	1 cut(s)	220
XceI	RCATGY	3 cut(s)	740, 796, 1009
XcmI	CCANNNNNNNNNTGG	3 cut(s)	616, 671, 1084
XmiI	GTMKAC	1 cut(s)	201
XmnI	GAANNNNTTC	1 cut(s)	360
XspI	CTAG	3 cut(s)	486, 1085, 1421
Zsp2I	ATGCAT	2 cut(s)	798, 1011

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.