Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0010684

Physical Location & Seq

Genomic Coordinates Forward (+)

12606216 .. 12612571

Gene Structure 6356 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1136800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2439 bp

ATGGCCATGGCGGTGGCTCACCACAGAGAGAGCAGCAGTGGGAGCAGTATAAACAAGCACCTCGATGCCGGAAAATATGTACGTTACACATCCGAGCAAGTTGAAGCTCTTGAGAGAGTCTACGCCGAGTGCCCGAAGCCGAGCTCTCTGCGCAGGCAACAGTTGATCCGGGAGTGCTCCATTTTATCAAACATTGAGCCCAGACAGATCAAGGTCTGGTTCCAGAACCGCAGGTGTAGGGAGAAGCAGAGAAAAGAGTCCTCAAGACTGCAGACTGTAAACAGAAAATTATCGGCAATGAACAAACTGCTGATGGAGGAAAATGATCGATTGCAGAAACAGGTCTCTCAGCTTGTTTGTGAAAATGGTTATATGCGCCAGCAATTGCATAGTGCATCAGCTGCAACTACTGATGCAAGCGGCGACTCTGTGGTGACAACTCCTCAGCATTCTCTCAGAGATGCTAATAACCCTGCAGGACTCCTATCCGTAGCGGAGGAGACATTGGCAGAGTTCCTATCCAAGGCTACCGGAACTGCTGTCGATTGGGTCCAGATGCCTGGGATGAAGCCTGGTCCGGATTCAGTTGGGATATTTGCCATTTCACAAAGTTGCAGTGGAGTGGCAGCAAGAGCCTGCGGCCTCGTAAGTTTAGAGCCTACGAAGATTGCAGAGATCCTTAAAGATCGTCCATCTTGGTTCCGGGACTGTCGGAGCCTTGAAGTTTTTACAATGTTTCCTGCTGGAAATGGAGGAACTATAGAACTTATATATACACAGACATATGCTCCAACTACATTGGCTCCTGCAAGGGATTTTTGGACCCTAAGATACACTGCAACTTTAGACAATGGCAGTTTTGTGGTGTGTGAAAGATCTCTTTCCGGTTCTGGCGCTGGCCCAAATCCTGCTTCTGCTTCACAGTTTGTTAGAGGCGCGATGCTTCCTAGTGGTTATCTGATTCGACCATGTGAGGGTGGGGGATCAATCGTTCATATTGTTGACCACCTGAATCTTGAGGCATGGAGTGTGCCAGAGGTGCTGAGACCGCTTTATGAATCGTCTAAAGTGGTAGCACAGAGAATGACTATTGCTGCGTTGCGCTATATCAGACAAATAGCCGAAGAGACAAGCGGTGAAGTGGTGTACAGTTTGGGTAGGCAGCCAGCTGTTCTGCGAACTTTTAGCCAAAGATTGATCAGAGGCTTCAATGATGCTGTCAATGGATTCAATGATGATGGATGGTCATTGGTCAACTGTGATGGTGATGGTGCTGACGATGTTATAATTGCAGTCAATTCAACTAAGAATTTAACTTCTACTTCGAACCATGCCAATTCCCTTGCATTACTTGGAGGTGTTCTCTGTGCAAAGGCTTCCATGTTACTACAAAATGTGCCCCCTGCTGTTCTTGTTCGTTTCTTGAGGGAGCACCGTTCAGAGTGGGCAGATTTCAATGTTGATGCCTACTCCGCCACATCTATGAAAGCTGGTGCATATGCTTATCCTGGGATGAGGCCAACAAGGTTTACCGGGGGACAAATTATCATGCCACTTGGCCACACAATTGAACAAGAAGAGTTGCTTGAAGTTGTTCGACTTGAAGGCCATTCTCTAACTCAAGAGGATGCTTTTGCATCGAGGGACATTCATCTCTTGCAGATATGTAGTGGAGTTGATGAGAATGCTGTCGGAGCCTGCTCTGAGCTTGTTTTTGCTCCAATTGATGAGATGTTTCCAGATGATGCGCCATTGCTTCCCTCTGGTTTCCGTATTATCCCACTGGATTCAAAAACAGGTGATTCAAAGGATACTTTGAATACACATCGAACATTAGATTTGACATCCAGTCTTGAAGTGGGCTCAACAACGAGCAATGCAGCTGGAGAGCTTACTACATTTCACAATACTCGATCAGTATTAACGATCGCCTTCCAGTTTCCATTTGATAATAGTCTACAAGAGAATGTCGCAAACATGGCACGGCAGTATGTTCGAAGCGTAATTTCATCTGTGCAGAGAGTTGCGATGGCTATATCTCCATCTGGATTGAGTCCGTCCGTAGGACCAAAACTATCTCCCGGCTCCCCTGAAGCTCAAACACTGGCTCACTGGATCTGCCAGATTATCATGTTGGGGTCAATGCCGGTTTTCATCTTTGCAAACCAGGCAGGACTTGACATGCTGGAGACAACATTAGTTGCCCTTCAGGACATCTCATTGGACAAGATTTTCGATGAGTCCGGACGCAAGACGTTGTGTGCAGACTTTGCTAAGCTAATGAACCAGGGGTTTGCATACTTGCCGGCTGGCATCTGCATGTCGACGATGGGACGCCACGTATCGTACGAGCAAGCGATTGCATGGAAAGTAGTTGCAGCAGAAGAGAACTCTGTCCATTGTCTTGCCTTCTCCTTCGTAAACTGGTCTTTTGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001067 GO:0003002 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0007389 GO:0008150 GO:0009653 GO:0009798 GO:0009799 GO:0009855 GO:0009888 GO:0009889 GO:0009933 GO:0009943 GO:0009944 GO:0009955 GO:0009956 GO:0009987 GO:0010014 GO:0010051 GO:0010087 GO:0010089 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0048507 GO:0048532 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065001 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Homeobox MEKHLA START

Protein Analysis

813

Amino Acids

88.65

Weight (kDa)

5.85

Isoelectric Point (pI)

45.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Homeodomain	PF00046	25 - 83	1e-15	Homeodomain
START	PF01852	164 - 371	2.1e-47	START domain
MEKHLA	PF08670	714 - 811	4.9e-29	MEKHLA domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010684)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G60690
fragaria_vesca	FvH4_3g32190 FvH4_3g32190 FvH4_3g32190 FvH4_3g32190
malus_domestica	MD03G1118500.v1.1 MD11G1136800.v1.1
prunus_persica	Prupe.6G102300_v2.0.a1
pyrus_communis	pycom11g11330
rosa_chinensis	RchiOBHm_Chr5g0058801
rosa_laevigata	RLG00000035251
rosa_multiflora	Rmu_sc0005911.1_g000011 Rmu_sc0041020.1_g000002
rosa_roxburghii	Rroxscaffold_1G00021600
rosa_rugosa	Rorug05G0319200
rosa_samantha	Rh5AG384000 Rh5BG395900 Rh5CG419800 Rh5DG411600
rosa_wichuraiana	Rw5G036180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1286
AarI	CACCTGC	1 cut(s)	222
Acc16I	TGCGCA	1 cut(s)	152
Acc36I	ACCTGC	1 cut(s)	222
AccI	GTMKAC	3 cut(s)	120, 1957, 2324
AccII	CGCG	1 cut(s)	938
AccIII	TCCGGA	2 cut(s)	577, 2243
AciI	CCGC	8 cut(s)	11, 229, 420, 494, 639, 1049, 1134, 1473
AclWI	GGATC	4 cut(s)	160, 670, 991, 2123
AcoI	YGGCCR	2 cut(s)	3, 1558
AcsI	RAATTY	1 cut(s)	1309
AcuI	CTGAAG	2 cut(s)	2112, 2192
AcyI	GRCGYC	1 cut(s)	2335
AfaI	GTAC	3 cut(s)	81, 1148, 2348
AfiI	CCNNNNNNNGG	3 cut(s)	523, 974, 2063
AhdI	GACNNNNNGTC	1 cut(s)	1848
AjnI	CCWGG	5 cut(s)	559, 571, 1507, 2166, 2286
AjuI	GAANNNNNNNTTGG	2 cut(s)	895, 927
AloI	GAACNNNNNNTCC	2 cut(s)	975, 1007
Alw21I	GWGCWC	3 cut(s)	146, 179, 1434
Alw26I	GTCTC	5 cut(s)	349, 494, 1039, 1121, 2183
AlwI	GGATC	4 cut(s)	160, 670, 991, 2123
Aor13HI	TCCGGA	2 cut(s)	577, 2243
AoxI	GGCC	6 cut(s)	3, 640, 898, 1517, 1558, 1606
ApeKI	GCWGC	7 cut(s)	33, 401, 626, 1094, 1162, 1880, 2378
ApoI	RAATTY	1 cut(s)	1309
ArsI	GACNNNNNNTTYG	2 cut(s)	2216, 2248
Asp700I	GAANNNNTTC	1 cut(s)	1593
AspLEI	GCGC	6 cut(s)	153, 378, 896, 938, 1104, 1750
AspS9I	GGNCC	5 cut(s)	550, 575, 822, 899, 2066
AsuC2I	CCSGG	4 cut(s)	170, 704, 1534, 2082
AsuHPI	GGTGA	5 cut(s)	11, 445, 1148, 1277, 1811
AsuII	TTCGAA	2 cut(s)	1325, 1996
AvaII	GGWCC	4 cut(s)	550, 575, 822, 2066
BaeGI	GKGCMC	2 cut(s)	134, 1401
BalI	TGGCCA	2 cut(s)	5, 1560
BanII	GRGCYC	3 cut(s)	146, 201, 1865
Bbv12I	GWGCWC	3 cut(s)	146, 179, 1434
BbvCI	CCTCAGC	1 cut(s)	444
BbvI	GCAGC	7 cut(s)	45, 388, 638, 1081, 1174, 1892, 2390
BccI	CCATC	9 cut(s)	307, 700, 1232, 1236, 1256, 1262, 2023, 2050, 2323
BceAI	ACGGC	1 cut(s)	2000
BcgI	CGANNNNNNTGC	4 cut(s)	130, 164, 1976, 2010
BciT130I	CCWGG	5 cut(s)	561, 573, 1509, 2168, 2288
BciVI	GTATCC	1 cut(s)	1804
BclI	TGATCA	1 cut(s)	1197
BcnI	CCSGG	4 cut(s)	170, 704, 1534, 2082
BcoDI	GTCTC	5 cut(s)	349, 494, 1039, 1121, 2183
BfaI	CTAG	1 cut(s)	948
BfmI	CTRYAG	3 cut(s)	269, 474, 759
BfoI	RGCGCY	1 cut(s)	897
BfuAI	ACCTGC	1 cut(s)	222
BfuI	GTATCC	1 cut(s)	1804
BglII	AGATCT	1 cut(s)	875
BisI	GCNGC	9 cut(s)	34, 402, 421, 627, 640, 1095, 1163, 1881, 2379
BlpI	GCTNAGC	1 cut(s)	2274
BlsI	GCNGC	9 cut(s)	35, 403, 422, 628, 641, 1096, 1164, 1882, 2380
Bme1390I	CCNGG	9 cut(s)	170, 561, 573, 704, 1509, 1534, 2082, 2168, 2288
Bme18I	GGWCC	4 cut(s)	550, 575, 822, 2066
BmeRI	GACNNNNNGTC	1 cut(s)	1848
BmgT120I	GGNCC	5 cut(s)	550, 575, 822, 899, 2066
BmiI	GGNNCC	8 cut(s)	221, 551, 701, 716, 804, 824, 1696, 2086
BmrFI	CCNGG	9 cut(s)	170, 561, 573, 704, 1509, 1534, 2082, 2168, 2288
BplI	GAGNNNNNCTC	2 cut(s)	1347, 1379
BpmI	CTGGAG	2 cut(s)	1905, 2207
Bpu10I	CCTNAGC	1 cut(s)	444
Bpu1102I	GCTNAGC	1 cut(s)	2274
Bpu14I	TTCGAA	2 cut(s)	1325, 1996
BpuEI	CTTGAG	5 cut(s)	131, 247, 1037, 1444, 1605
BpuMI	CCSGG	4 cut(s)	170, 704, 1534, 2082
Bsa29I	ATCGAT	1 cut(s)	328
BsaAI	YACGTR	1 cut(s)	2341
BsaHI	GRCGYC	1 cut(s)	2335
BsaI	GGTCTC	2 cut(s)	349, 1039
BsaJI	CCNNGG	6 cut(s)	6, 522, 560, 1508, 1533, 2287
BsaWI	WCCGGW	4 cut(s)	530, 577, 884, 2243
BsaXI	ACNNNNNCTCC	4 cut(s)	772, 787, 802, 817
Bsc4I	CCNNNNNNNGG	3 cut(s)	523, 974, 2063
Bse118I	RCCGGY	2 cut(s)	2146, 2305
Bse1I	ACTGG	6 cut(s)	1788, 1848, 1936, 2109, 2117, 2429
Bse3DI	GCAATG	3 cut(s)	303, 1751, 1882
BseAI	TCCGGA	2 cut(s)	577, 2243
BseBI	CCWGG	5 cut(s)	561, 573, 1509, 2168, 2288
BseCI	ATCGAT	1 cut(s)	328
BseDI	CCNNGG	6 cut(s)	6, 522, 560, 1508, 1533, 2287
BseGI	GGATG	6 cut(s)	89, 570, 1247, 1518, 1633, 1844
BseLI	CCNNNNNNNGG	3 cut(s)	523, 974, 2063
BseMI	GCAATG	3 cut(s)	303, 1751, 1882
BseMII	CTCAG	5 cut(s)	362, 458, 469, 1034, 1695
BseNI	ACTGG	6 cut(s)	1788, 1848, 1936, 2109, 2117, 2429
BseRI	GAGGAG	2 cut(s)	432, 512
BseSI	GKGCMC	2 cut(s)	134, 1401
BseXI	GCAGC	7 cut(s)	45, 388, 638, 1081, 1174, 1892, 2390
BsgI	GTGCAG	2 cut(s)	2036, 2283
Bsh1236I	CGCG	1 cut(s)	938
Bsh1285I	CGRYCG	1 cut(s)	1929
BshFI	GGCC	6 cut(s)	5, 642, 900, 1519, 1560, 1608
BshVI	ATCGAT	1 cut(s)	328
BsiEI	CGRYCG	1 cut(s)	1929
BsiHKAI	GWGCWC	3 cut(s)	146, 179, 1434
BsiWI	CGTACG	1 cut(s)	2346
BslFI	GGGAC	4 cut(s)	719, 1551, 1658, 2346
BslI	CCNNNNNNNGG	3 cut(s)	523, 974, 2063
BsmAI	GTCTC	5 cut(s)	349, 494, 1039, 1121, 2183
BsmFI	GGGAC	4 cut(s)	719, 1551, 1658, 2346
BsmI	GAATGC	2 cut(s)	448, 1690
BsnI	GGCC	6 cut(s)	5, 642, 900, 1519, 1560, 1608
Bso31I	GGTCTC	2 cut(s)	349, 1039
Bsp119I	TTCGAA	2 cut(s)	1325, 1996
Bsp1286I	GDGCHC	7 cut(s)	134, 146, 179, 201, 1401, 1434, 1865
Bsp13I	TCCGGA	2 cut(s)	577, 2243
Bsp1407I	TGTACA	1 cut(s)	1146
Bsp1720I	GCTNAGC	1 cut(s)	2274
Bsp19I	CCATGG	1 cut(s)	6
BspACI	CCGC	8 cut(s)	11, 229, 420, 494, 639, 1049, 1134, 1473
BspANI	GGCC	6 cut(s)	5, 642, 900, 1519, 1560, 1608
BspCNI	CTCAG	5 cut(s)	361, 457, 468, 1035, 1696
BspDI	ATCGAT	1 cut(s)	328
BspEI	TCCGGA	2 cut(s)	577, 2243
BspFNI	CGCG	1 cut(s)	938
BspLI	GGNNCC	8 cut(s)	221, 551, 701, 716, 804, 824, 1696, 2086
BspMAI	CTGCAG	2 cut(s)	273, 478
BspMI	ACCTGC	1 cut(s)	222
BspPI	GGATC	4 cut(s)	160, 670, 991, 2123
BspT104I	TTCGAA	2 cut(s)	1325, 1996
BspTNI	GGTCTC	2 cut(s)	349, 1039
BsrDI	GCAATG	3 cut(s)	303, 1751, 1882
BsrFI	RCCGGY	2 cut(s)	2146, 2305
BsrGI	TGTACA	1 cut(s)	1146
BsrI	ACTGG	6 cut(s)	1788, 1848, 1936, 2109, 2117, 2429
BssAI	RCCGGY	2 cut(s)	2146, 2305
BssECI	CCNNGG	6 cut(s)	6, 522, 560, 1508, 1533, 2287
BssNI	GRCGYC	1 cut(s)	2335
BssT1I	CCWWGG	2 cut(s)	6, 522
Bst2UI	CCWGG	5 cut(s)	561, 573, 1509, 2168, 2288
Bst4CI	ACNGT	7 cut(s)	162, 277, 710, 924, 1151, 1259, 1436
Bst6I	CTCTTC	3 cut(s)	1119, 1572, 2379
BstACI	GRCGYC	1 cut(s)	2335
BstAPI	GCANNNNNTGC	2 cut(s)	401, 2270
BstAUI	TGTACA	1 cut(s)	1146
BstBAI	YACGTR	1 cut(s)	2341
BstBI	TTCGAA	2 cut(s)	1325, 1996
BstDEI	CTNAG	8 cut(s)	348, 444, 455, 827, 1043, 1305, 1704, 2274
BstDSI	CCRYGG	1 cut(s)	6
BstENI	CCTNNNNNAGG	1 cut(s)	521
BstF5I	GGATG	6 cut(s)	89, 570, 1247, 1518, 1633, 1844
BstFNI	CGCG	1 cut(s)	938
BstH2I	RGCGCY	1 cut(s)	897
BstHHI	GCGC	6 cut(s)	153, 378, 896, 938, 1104, 1750
BstMAI	GTCTC	5 cut(s)	349, 494, 1039, 1121, 2183
BstMCI	CGRYCG	1 cut(s)	1929
BstNI	CCWGG	5 cut(s)	561, 573, 1509, 2168, 2288
BstNSI	RCATGY	2 cut(s)	2185, 2323
BstSCI	CCNGG	9 cut(s)	168, 559, 571, 702, 1507, 1532, 2080, 2166, 2286
BstSFI	CTRYAG	3 cut(s)	269, 474, 759
BstSLI	GKGCMC	2 cut(s)	134, 1401
BstUI	CGCG	1 cut(s)	938
BstV1I	GCAGC	7 cut(s)	45, 388, 638, 1081, 1174, 1892, 2390
BstX2I	RGATCY	3 cut(s)	675, 875, 2115
BstXI	CCANNNNNNTGG	2 cut(s)	13, 560
BstYI	RGATCY	3 cut(s)	675, 875, 2115
Bsu15I	ATCGAT	1 cut(s)	328
BsuI	GTATCC	1 cut(s)	1804
BsuRI	GGCC	6 cut(s)	5, 642, 900, 1519, 1560, 1608
BsuTUI	ATCGAT	1 cut(s)	328
BtgI	CCRYGG	1 cut(s)	6
BtgZI	GCGATG	2 cut(s)	953, 2042
BtsCI	GGATG	6 cut(s)	89, 570, 1247, 1518, 1633, 1844
BtsI	GCAGTG	3 cut(s)	43, 622, 834
BtsIMutI	CAGTG	6 cut(s)	43, 622, 834, 1781, 2102, 2110
BveI	ACCTGC	1 cut(s)	222
CfoI	GCGC	6 cut(s)	153, 378, 896, 938, 1104, 1750
Cfr10I	RCCGGY	2 cut(s)	2146, 2305
Cfr13I	GGNCC	5 cut(s)	550, 575, 822, 899, 2066
ClaI	ATCGAT	1 cut(s)	328
CseI	GACGC	2 cut(s)	2256, 2343
Csp6I	GTAC	3 cut(s)	80, 1147, 2347
CviQI	GTAC	3 cut(s)	80, 1147, 2347
DdeI	CTNAG	8 cut(s)	348, 444, 455, 827, 1043, 1305, 1704, 2274
DriI	GACNNNNNGTC	1 cut(s)	1848
EaeI	YGGCCR	2 cut(s)	3, 1558
Eam1104I	CTCTTC	3 cut(s)	1119, 1572, 2379
Eam1105I	GACNNNNNGTC	1 cut(s)	1848
EarI	CTCTTC	3 cut(s)	1119, 1572, 2379
EciI	GGCGGA	1 cut(s)	1462
Ecl136II	GAGCTC	1 cut(s)	144
Eco130I	CCWWGG	2 cut(s)	6, 522
Eco24I	GRGCYC	3 cut(s)	146, 201, 1865
Eco31I	GGTCTC	2 cut(s)	349, 1039
Eco47I	GGWCC	4 cut(s)	550, 575, 822, 2066
Eco53kI	GAGCTC	1 cut(s)	144
Eco57I	CTGAAG	2 cut(s)	2112, 2192
EcoICRI	GAGCTC	1 cut(s)	144
EcoNI	CCTNNNNNAGG	1 cut(s)	521
EcoRII	CCWGG	5 cut(s)	559, 571, 1507, 2166, 2286
EcoT14I	CCWWGG	2 cut(s)	6, 522
EcoT38I	GRGCYC	3 cut(s)	146, 201, 1865
ErhI	CCWWGG	2 cut(s)	6, 522
FalI	AAGNNNNNCTT	2 cut(s)	1569, 1601
FaqI	GGGAC	4 cut(s)	719, 1551, 1658, 2346
FauNDI	CATATG	2 cut(s)	784, 1498
FbaI	TGATCA	1 cut(s)	1197
FblI	GTMKAC	3 cut(s)	120, 1957, 2324
Fnu4HI	GCNGC	9 cut(s)	34, 402, 421, 627, 640, 1095, 1163, 1881, 2379
FokI	GGATG	6 cut(s)	76, 577, 1254, 1525, 1640, 1831
FriOI	GRGCYC	3 cut(s)	146, 201, 1865
Fsp4HI	GCNGC	9 cut(s)	34, 402, 421, 627, 640, 1095, 1163, 1881, 2379
FspBI	CTAG	1 cut(s)	948
FspI	TGCGCA	1 cut(s)	152
GlaI	GCGC	6 cut(s)	152, 377, 895, 937, 1103, 1749
GluI	GCNGC	9 cut(s)	34, 402, 421, 627, 640, 1095, 1163, 1881, 2379
GsuI	CTGGAG	2 cut(s)	1905, 2207
HaeII	RGCGCY	1 cut(s)	897
HaeIII	GGCC	6 cut(s)	5, 642, 900, 1519, 1560, 1608
HgaI	GACGC	2 cut(s)	2256, 2343
HhaI	GCGC	6 cut(s)	153, 378, 896, 938, 1104, 1750
Hin1I	GRCGYC	1 cut(s)	2335
Hin6I	GCGC	6 cut(s)	151, 376, 894, 936, 1102, 1748
HinP1I	GCGC	6 cut(s)	151, 376, 894, 936, 1102, 1748
HincII	GTYRAC	3 cut(s)	1003, 1255, 2325
HindII	GTYRAC	3 cut(s)	1003, 1255, 2325
HphI	GGTGA	5 cut(s)	11, 445, 1148, 1277, 1811
Hpy166II	GTNNAC	9 cut(s)	121, 280, 1003, 1147, 1255, 1530, 1958, 2325, 2422
Hpy188I	TCNGA	9 cut(s)	94, 458, 714, 960, 1112, 1202, 1441, 1694, 1705
Hpy8I	GTNNAC	9 cut(s)	121, 280, 1003, 1147, 1255, 1530, 1958, 2325, 2422
Hpy99I	CGWCG	1 cut(s)	2329
HpyAV	CCTTC	5 cut(s)	1598, 1942, 2216, 2419, 2425
HpyCH4III	ACNGT	7 cut(s)	162, 277, 710, 924, 1151, 1259, 1436
HpyCH4IV	ACGT	3 cut(s)	82, 2255, 2340
HpyF3I	CTNAG	8 cut(s)	348, 444, 455, 827, 1043, 1305, 1704, 2274
HpySE526I	ACGT	3 cut(s)	82, 2255, 2340
Hsp92I	GRCGYC	1 cut(s)	2335
HspAI	GCGC	6 cut(s)	151, 376, 894, 936, 1102, 1748
Kpn2I	TCCGGA	2 cut(s)	577, 2243
KroI	GCCGGC	1 cut(s)	2305
KroNI	GCCGGC	1 cut(s)	2307
Ksp22I	TGATCA	1 cut(s)	1197
LmnI	GCTCC	9 cut(s)	42, 182, 714, 793, 808, 1429, 1694, 1723, 2090
Lsp1109I	GCAGC	7 cut(s)	45, 388, 638, 1081, 1174, 1892, 2390
MaeI	CTAG	1 cut(s)	948
MaeII	ACGT	3 cut(s)	82, 2255, 2340
MaeIII	GTNAC	3 cut(s)	83, 433, 1383
MboII	GAAGA	4 cut(s)	676, 1136, 1589, 2396
MfeI	CAATTG	3 cut(s)	383, 1566, 1722
MflI	RGATCY	3 cut(s)	675, 875, 2115
MhlI	GDGCHC	7 cut(s)	134, 146, 179, 201, 1401, 1434, 1865
MlsI	TGGCCA	2 cut(s)	5, 1560
MluNI	TGGCCA	2 cut(s)	5, 1560
MlyI	GAGTC	6 cut(s)	126, 266, 419, 474, 2062, 2249
MmeI	TCCRAC	3 cut(s)	692, 815, 1672
Mox20I	TGGCCA	2 cut(s)	5, 1560
MroI	TCCGGA	2 cut(s)	577, 2243
MroNI	GCCGGC	1 cut(s)	2305
MroXI	GAANNNNTTC	1 cut(s)	1593
MscI	TGGCCA	2 cut(s)	5, 1560
MseI	TTAA	3 cut(s)	681, 1313, 1922
MslI	CAYNNNNRTG	4 cut(s)	11, 63, 1481, 2318
Msp20I	TGGCCA	2 cut(s)	5, 1560
MspA1I	CMGCKG	3 cut(s)	401, 1169, 1883
MspR9I	CCNGG	9 cut(s)	170, 561, 573, 704, 1509, 1534, 2082, 2168, 2288
MunI	CAATTG	3 cut(s)	383, 1566, 1722
Mva1269I	GAATGC	2 cut(s)	448, 1690
MvaI	CCWGG	5 cut(s)	561, 573, 1509, 2168, 2288
MvnI	CGCG	1 cut(s)	938
NaeI	GCCGGC	1 cut(s)	2307
NciI	CCSGG	4 cut(s)	170, 704, 1534, 2082
NcoI	CCATGG	1 cut(s)	6
NdeI	CATATG	2 cut(s)	784, 1498
NgoMIV	GCCGGC	1 cut(s)	2305
NlaIV	GGNNCC	8 cut(s)	221, 551, 701, 716, 804, 824, 1696, 2086
NmeAIII	GCCGAG	2 cut(s)	151, 165
NmuCI	GTSAC	1 cut(s)	433
NsbI	TGCGCA	1 cut(s)	152
NspI	RCATGY	2 cut(s)	2185, 2323
NspV	TTCGAA	2 cut(s)	1325, 1996
PaqCI	CACCTGC	1 cut(s)	222
PcsI	WCGNNNNNNNCGW	1 cut(s)	2346
PctI	GAATGC	2 cut(s)	448, 1690
PdiI	GCCGGC	1 cut(s)	2307
PdmI	GAANNNNTTC	1 cut(s)	1593
PfeI	GAWTC	7 cut(s)	581, 961, 1012, 1058, 1227, 1787, 1802
Pfl23II	CGTACG	1 cut(s)	2346
PfoI	TCCNGGA	2 cut(s)	168, 702
PkrI	GCNGC	9 cut(s)	35, 403, 422, 628, 641, 1096, 1164, 1882, 2380
Ple19I	CGATCG	1 cut(s)	1929
PleI	GAGTC	6 cut(s)	125, 265, 419, 474, 2061, 2248
PpsI	GAGTC	6 cut(s)	125, 265, 419, 474, 2061, 2248
Ppu21I	YACGTR	1 cut(s)	2341
PsiI	TTATAA	1 cut(s)	1286
Psp124BI	GAGCTC	1 cut(s)	146
Psp6I	CCWGG	5 cut(s)	559, 571, 1507, 2166, 2286
PspGI	CCWGG	5 cut(s)	559, 571, 1507, 2166, 2286
PspLI	CGTACG	1 cut(s)	2346
PspN4I	GGNNCC	8 cut(s)	221, 551, 701, 716, 804, 824, 1696, 2086
PspPI	GGNCC	5 cut(s)	550, 575, 822, 899, 2066
PstI	CTGCAG	2 cut(s)	273, 478
PsuI	RGATCY	3 cut(s)	675, 875, 2115
PvuI	CGATCG	1 cut(s)	1929
PvuII	CAGCTG	3 cut(s)	401, 1169, 1883
RsaI	GTAC	3 cut(s)	81, 1148, 2348
RsaNI	GTAC	3 cut(s)	80, 1147, 2347
RseI	CAYNNNNRTG	4 cut(s)	11, 63, 1481, 2318
SacI	GAGCTC	1 cut(s)	146
SalI	GTCGAC	1 cut(s)	2323
SaqAI	TTAA	3 cut(s)	681, 1313, 1922
SatI	GCNGC	9 cut(s)	34, 402, 421, 627, 640, 1095, 1163, 1881, 2379
Sau96I	GGNCC	5 cut(s)	550, 575, 822, 899, 2066
SbfI	CCTGCAGG	1 cut(s)	478
SchI	GAGTC	6 cut(s)	126, 266, 419, 474, 2062, 2249
ScrFI	CCNGG	9 cut(s)	170, 561, 573, 704, 1509, 1534, 2082, 2168, 2288
SdaI	CCTGCAGG	1 cut(s)	478
SduI	GDGCHC	7 cut(s)	134, 146, 179, 201, 1401, 1434, 1865
SfcI	CTRYAG	3 cut(s)	269, 474, 759
SfuI	TTCGAA	2 cut(s)	1325, 1996
SinI	GGWCC	4 cut(s)	550, 575, 822, 2066
SmiMI	CAYNNNNRTG	4 cut(s)	11, 63, 1481, 2318
SmlI	CTYRAG	5 cut(s)	110, 262, 1016, 1423, 1620
SmoI	CTYRAG	5 cut(s)	110, 262, 1016, 1423, 1620
Sse8387I	CCTGCAGG	1 cut(s)	478
SsiI	CCGC	8 cut(s)	11, 229, 420, 494, 639, 1049, 1134, 1473
SspMI	CTAG	1 cut(s)	948
SstI	GAGCTC	1 cut(s)	146
StyD4I	CCNGG	9 cut(s)	168, 559, 571, 702, 1507, 1532, 2080, 2166, 2286
StyI	CCWWGG	2 cut(s)	6, 522
TaaI	ACNGT	7 cut(s)	162, 277, 710, 924, 1151, 1259, 1436
TaiI	ACGT	3 cut(s)	85, 2258, 2343
TatI	WGTACW	1 cut(s)	1146
TauI	GCSGC	2 cut(s)	423, 642
TfiI	GAWTC	7 cut(s)	581, 961, 1012, 1058, 1227, 1787, 1802
Tru1I	TTAA	3 cut(s)	681, 1313, 1922
Tru9I	TTAA	3 cut(s)	681, 1313, 1922
TscAI	CASTG	6 cut(s)	43, 622, 841, 1788, 2109, 2117
TseFI	GTSAC	1 cut(s)	433
TseI	GCWGC	7 cut(s)	33, 401, 626, 1094, 1162, 1880, 2378
Tsp45I	GTSAC	1 cut(s)	433
TspDTI	ATGAA	9 cut(s)	314, 581, 983, 1071, 1499, 1640, 1998, 2143, 2297
TspGWI	ACGGA	4 cut(s)	478, 1760, 2046, 2050
TspRI	CASTG	6 cut(s)	43, 622, 841, 1788, 2109, 2117
VpaK11BI	GGWCC	4 cut(s)	550, 575, 822, 2066
XagI	CCTNNNNNAGG	1 cut(s)	521
XapI	RAATTY	1 cut(s)	1309
XceI	RCATGY	2 cut(s)	2185, 2323
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1855
XmiI	GTMKAC	3 cut(s)	120, 1957, 2324
XmnI	GAANNNNTTC	1 cut(s)	1593
XspI	CTAG	1 cut(s)	948

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.